pycom09g00380

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013199
Physical Location & Seq
Reverse (-)
318903 .. 320649
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g00380.1

Sequence Viewer

Length: 1395 bp
ATGCCTGAATTTACTCATCTGTGCTCCTCCAACAAGCTGTTTCCTTCGCCAAGCAGAAGCCATGGAAGATTGCTGAATCATGAGGAAACCCATCTCAAATCACAAGCTGTGTCTGCTGTAACGAATTCGAAATCTATGGGAAATGGGGTTTCGAGTGTAGATGATAGGTGCCTGAAGCAAGTGTCTCCGCTTAGAGGTGGCGGGTCGAGGAACACGAGTCCAGTGGGTTGTGTAAGATCAAGGAATACAAGTCCTTCTAGGCAAAAGGTGGTTAAGACAAAGCCTCGAGGACTAGATGAAGAAACGGCTTCTACATTTGGGAAACCAGTTCAACCTGATGTTCACATGGAGGATAATATATGGGCAATGTTACCCGAGGATTTGTTGAATGAGATTTTAGCTAGAATTCCGCCGTTCATGATTTTTAGGCTCCGCTGTGTATGTAAACGGTGGAATACAATTCTACAAGATAGAAGCTTTCTTAAGTTTCACTCACAAGTGCCGTCTCATGGGCCTTGTCTTCTCACATTCTGGAAGAACTCTCAGACCCCACAATGCTCAGTCTTCAGCTTGCCATTAAAAACGTGGTACAGGATTCCATTCACATTTTTGCCACAGTGGGCGTTTTGGTTAGTTGGTTCTTCGGCTGGCTTAGTTTGCTTTTCCGGGCTTGATGGCTTAAATTTTAGAGCCTTGGTTTGTAACCCTCTTACACAAACTTGGAGGACATTACCAAGTATGCATTATAATCAGCAAAGGCAGCTAATAATGGTTGTTGATAGGAAGGACCGGTCGTTTAAAGTTATAGCTACTAGTGATATTTATGGTGACAAATCACTGCCCACTGAAGTATATGATTCGAAGCTCAATACATGGTCAATTCACCAGATAATGCCTGCAGTTAATCTTTGCTCGTCAAAGATGGCATATTGTGACTCCAGATTATATTTGGAAACCCTTTCTCCACTTGGCTTGATGATGTATCGACTGGACACAGGGTACTGGGAACACATTCCAGCTAAGTTCCCGCGGTCTCTATTGGATGGTTATTTGGTTGCTGGCACCCAGAAGCGTCTGTTTCTAGTTGGAAGGATTGGTCTTTATAGTACTCTTCAGAGTATGAGAATCTGGGAACTGGATCATGCTAAATTTGTATGGGTGGAGATTAGCAGGATGCCTCCAAAATATTTCCGATCTCTGTTAAGGTTATCAGCTGAGAGGTTCGAGTGCTCTGGACAGGATAACTTGATTTGCTTCACATCTTGGAACCAAGGGAAGGGTCTTCTTTACGACGTTGATAAGAAGGTGTGGTCATGGATAGCAGGCTGTGCTCTGCAGTCATACAACAGCCAGGTTTGTTTCTATGAGCCGAGATTTGATGCTTCCATATACTAA

Protein Analysis

465

Amino Acids

53.15

Weight (kDa)

9.32

Isoelectric Point (pI)

53.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 121 - 159 1e-06 F-box-like
F-box PF00646 122 - 162 2.1e-09 F-box domain
Beta-prop_KIB1-4 PF03478 189 - 420 3.3e-07 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 205 - 318 8.8e-06 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15710 AT5G15710
fragaria_vesca FvH4_6g46160
malus_domestica MD09G1076600.v1.1
prunus_persica Prupe.3G247500_v2.0.a1 Prupe.3G247500_v2.0.a1
pyrus_communis pycom09g00380 pycom17g06740
rosa_chinensis RchiOBHm_Chr2g0164611
rosa_laevigata RLG00000021469
rosa_multiflora Rmu_ssc0000076.1_g000006
rosa_roxburghii Rroxscaffold_2G00086460
rosa_rugosa Rorug02G0509400
rosa_samantha Rh2AG577000 Rh2BG588100 Rh2CG558900 Rh2DG598700
rosa_wichuraiana Rw2G047910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AbsI CCTCGAGG 1 cut(s) 285
AccB1I GGYRCC 2 cut(s) 168, 1061
AccII CGCG 1 cut(s) 1030
AciI CCGC 6 cut(s) 188, 201, 410, 433, 1028, 1030
AclWI GGATC 1 cut(s) 1146
AcsI RAATTY 5 cut(s) 8, 124, 405, 682, 1148
AcuI CTGAAG 4 cut(s) 194, 550, 867, 1097
AfaI GTAC 3 cut(s) 590, 1001, 1108
AfiI CCNNNNNNNGG 3 cut(s) 194, 509, 1276
AflII CTTAAG 1 cut(s) 482
AgeI ACCGGT 1 cut(s) 789
AgsI TTSAA 2 cut(s) 332, 388
AhlI ACTAGT 1 cut(s) 812
AjnI CCWGG 1 cut(s) 1350
Alw21I GWGCWC 3 cut(s) 26, 1232, 1333
Alw26I GTCTC 3 cut(s) 189, 510, 1038
AlwI GGATC 1 cut(s) 1146
Ama87I CYCGRG 2 cut(s) 285, 374
AoxI GGCC 1 cut(s) 512
ApeKI GCWGC 1 cut(s) 760
ApoI RAATTY 5 cut(s) 8, 124, 405, 682, 1148
AsiGI ACCGGT 1 cut(s) 789
Asp700I GAANNNNTTC 1 cut(s) 1011
AspS9I GGNCC 2 cut(s) 512, 787
AsuC2I CCSGG 1 cut(s) 667
AsuHPI GGTGA 2 cut(s) 839, 875
AsuII TTCGAA 2 cut(s) 128, 860
AvaI CYCGRG 2 cut(s) 285, 374
AvaII GGWCC 1 cut(s) 787
BanI GGYRCC 2 cut(s) 168, 1061
BauI CACGAG 1 cut(s) 214
BbsI GAAGAC 3 cut(s) 512, 556, 1274
Bbv12I GWGCWC 3 cut(s) 26, 1232, 1333
BbvI GCAGC 1 cut(s) 772
BccI CCATC 4 cut(s) 99, 668, 916, 1037
BceAI ACGGC 3 cut(s) 321, 397, 487
BciT130I CCWGG 1 cut(s) 1352
BcnI CCSGG 1 cut(s) 667
BcoDI GTCTC 3 cut(s) 189, 510, 1038
BcuI ACTAGT 1 cut(s) 812
BfaI CTAG 5 cut(s) 258, 293, 402, 813, 1082
BfmI CTRYAG 2 cut(s) 897, 1334
BfrI CTTAAG 1 cut(s) 482
BisI GCNGC 1 cut(s) 761
BlsI GCNGC 1 cut(s) 762
BmcAI AGTACT 1 cut(s) 1108
Bme1390I CCNGG 2 cut(s) 667, 1352
Bme18I GGWCC 1 cut(s) 787
BmeT110I CYCGRG 2 cut(s) 285, 374
BmgT120I GGNCC 2 cut(s) 512, 787
BmiI GGNNCC 4 cut(s) 170, 431, 1063, 1268
BmrFI CCNGG 2 cut(s) 667, 1352
BmrI ACTGGG 1 cut(s) 1012
BmsI GCATC 2 cut(s) 1164, 1369
BmuI ACTGGG 1 cut(s) 1012
BpiI GAAGAC 3 cut(s) 512, 556, 1274
BpmI CTGGAG 1 cut(s) 922
Bpu14I TTCGAA 2 cut(s) 128, 860
BpuMI CCSGG 1 cut(s) 667
BsaI GGTCTC 1 cut(s) 1038
BsaJI CCNNGG 5 cut(s) 61, 375, 693, 1028, 1270
BsaWI WCCGGW 1 cut(s) 789
BsaXI ACNNNNNCTCC 2 cut(s) 946, 976
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 509, 1276
Bse118I RCCGGY 1 cut(s) 789
Bse1I ACTGG 5 cut(s) 221, 326, 993, 1007, 1140
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 1352
BseDI CCNNGG 5 cut(s) 61, 375, 693, 1028, 1270
BseGI GGATG 2 cut(s) 1048, 1179
BseLI CCNNNNNNNGG 3 cut(s) 194, 509, 1276
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 3 cut(s) 557, 573, 1206
BseNI ACTGG 5 cut(s) 221, 326, 993, 1007, 1140
BseRI GAGGAG 1 cut(s) 16
BseXI GCAGC 1 cut(s) 772
Bsh1236I CGCG 1 cut(s) 1030
Bsh1285I CGRYCG 1 cut(s) 794
BshFI GGCC 1 cut(s) 514
BshNI GGYRCC 2 cut(s) 168, 1061
BshTI ACCGGT 1 cut(s) 789
BsiEI CGRYCG 1 cut(s) 794
BsiHKAI GWGCWC 3 cut(s) 26, 1232, 1333
BsiHKCI CYCGRG 2 cut(s) 285, 374
BsiSI CCGG 2 cut(s) 666, 790
BslI CCNNNNNNNGG 3 cut(s) 194, 509, 1276
BsmAI GTCTC 3 cut(s) 189, 510, 1038
BsmBI CGTCTC 1 cut(s) 510
BsnI GGCC 1 cut(s) 514
Bso31I GGTCTC 1 cut(s) 1038
BsoBI CYCGRG 2 cut(s) 285, 374
Bsp119I TTCGAA 2 cut(s) 128, 860
Bsp1286I GDGCHC 3 cut(s) 26, 1232, 1333
Bsp143I GATC 3 cut(s) 236, 1138, 1193
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 6 cut(s) 188, 201, 410, 433, 1028, 1030
BspANI GGCC 1 cut(s) 514
BspCNI CTCAG 3 cut(s) 556, 572, 1207
BspFNI CGCG 1 cut(s) 1030
BspHI TCATGA 2 cut(s) 79, 417
BspLI GGNNCC 4 cut(s) 170, 431, 1063, 1268
BspMAI CTGCAG 2 cut(s) 901, 1338
BspPI GGATC 1 cut(s) 1146
BspT104I TTCGAA 2 cut(s) 128, 860
BspT107I GGYRCC 2 cut(s) 168, 1061
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 1038
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 789
BsrI ACTGG 5 cut(s) 221, 326, 993, 1007, 1140
BssAI RCCGGY 1 cut(s) 789
BssECI CCNNGG 5 cut(s) 61, 375, 693, 1028, 1270
BssMI GATC 3 cut(s) 236, 1138, 1193
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 3 cut(s) 61, 693, 1270
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 1352
Bst4CI ACNGT 2 cut(s) 450, 618
Bst6I CTCTTC 1 cut(s) 1116
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 1328
BstBI TTCGAA 2 cut(s) 128, 860
BstC8I GCNNGC 5 cut(s) 572, 649, 897, 1060, 1324
BstDEI CTNAG 6 cut(s) 191, 543, 559, 652, 1020, 1215
BstDSI CCRYGG 2 cut(s) 61, 1028
BstF5I GGATG 2 cut(s) 1048, 1179
BstFNI CGCG 1 cut(s) 1030
BstKTI GATC 3 cut(s) 239, 1141, 1196
BstMAI GTCTC 3 cut(s) 189, 510, 1038
BstMBI GATC 3 cut(s) 236, 1138, 1193
BstMCI CGRYCG 1 cut(s) 794
BstMWI GCNNNNNNNGC 4 cut(s) 113, 657, 760, 1328
BstNI CCWGG 1 cut(s) 1352
BstSCI CCNGG 2 cut(s) 665, 1350
BstSFI CTRYAG 2 cut(s) 897, 1334
BstUI CGCG 1 cut(s) 1030
BstV1I GCAGC 1 cut(s) 772
BstV2I GAAGAC 3 cut(s) 512, 556, 1274
BsuRI GGCC 1 cut(s) 514
BtgI CCRYGG 2 cut(s) 61, 1028
BtsCI GGATG 2 cut(s) 1048, 1179
BtsI GCAGTG 1 cut(s) 836
BtsIMutI CAGTG 4 cut(s) 228, 623, 836, 843
Cac8I GCNNGC 5 cut(s) 572, 649, 897, 1060, 1324
CciI TCATGA 2 cut(s) 79, 417
Cfr10I RCCGGY 1 cut(s) 789
Cfr13I GGNCC 2 cut(s) 512, 787
Cfr42I CCGCGG 1 cut(s) 1031
CseI GACGC 1 cut(s) 1061
Csp6I GTAC 3 cut(s) 589, 1000, 1107
CspAI ACCGGT 1 cut(s) 789
CspCI CAANNNNNGTGG 2 cut(s) 954, 989
CviAII CATG 8 cut(s) 62, 80, 346, 418, 509, 873, 1142, 1314
CviQI GTAC 3 cut(s) 589, 1000, 1107
DdeI CTNAG 6 cut(s) 191, 543, 559, 652, 1020, 1215
DpnI GATC 3 cut(s) 238, 1140, 1195
DpnII GATC 3 cut(s) 236, 1138, 1193
DraI TTTAAA 1 cut(s) 799
Eam1104I CTCTTC 1 cut(s) 1116
EarI CTCTTC 1 cut(s) 1116
EciI GGCGGA 1 cut(s) 399
Eco130I CCWWGG 3 cut(s) 61, 693, 1270
Eco31I GGTCTC 1 cut(s) 1038
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 4 cut(s) 194, 550, 867, 1097
Eco88I CYCGRG 2 cut(s) 285, 374
EcoRI GAATTC 2 cut(s) 124, 405
EcoRII CCWGG 1 cut(s) 1350
EcoT14I CCWWGG 3 cut(s) 61, 693, 1270
EcoT22I ATGCAT 1 cut(s) 744
ErhI CCWWGG 3 cut(s) 61, 693, 1270
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 8 cut(s) 65, 83, 349, 421, 512, 876, 1145, 1317
FatI CATG 8 cut(s) 61, 79, 345, 417, 508, 872, 1141, 1313
FauI CCCGC 2 cut(s) 194, 1035
Fnu4HI GCNGC 1 cut(s) 761
FokI GGATG 2 cut(s) 1055, 1186
Fsp4HI GCNGC 1 cut(s) 761
FspBI CTAG 5 cut(s) 258, 293, 402, 813, 1082
GluI GCNGC 1 cut(s) 761
GsuI CTGGAG 1 cut(s) 922
HaeIII GGCC 1 cut(s) 514
HapII CCGG 2 cut(s) 666, 790
HgaI GACGC 1 cut(s) 1061
Hin1II CATG 8 cut(s) 65, 83, 349, 421, 512, 876, 1145, 1317
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 6 cut(s) 76, 217, 595, 857, 935, 1125
HpaII CCGG 2 cut(s) 666, 790
HphI GGTGA 2 cut(s) 839, 875
Hpy166II GTNNAC 2 cut(s) 343, 446
Hpy188I TCNGA 3 cut(s) 546, 1116, 1193
Hpy188III TCNNGA 5 cut(s) 80, 418, 532, 939, 1233
Hpy8I GTNNAC 2 cut(s) 343, 446
Hpy99I CGWCG 1 cut(s) 1295
HpyAV CCTTC 6 cut(s) 54, 264, 778, 1083, 1270, 1297
HpyCH4III ACNGT 2 cut(s) 450, 618
HpyCH4IV ACGT 2 cut(s) 584, 1293
HpyCH4V TGCA 3 cut(s) 742, 899, 1336
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 657, 760, 1328
HpyF3I CTNAG 6 cut(s) 191, 543, 559, 652, 1020, 1215
HpySE526I ACGT 2 cut(s) 584, 1293
Hsp92II CATG 8 cut(s) 65, 83, 349, 421, 512, 876, 1145, 1317
KspI CCGCGG 1 cut(s) 1031
Kzo9I GATC 3 cut(s) 236, 1138, 1193
LmnI GCTCC 2 cut(s) 29, 435
Lsp1109I GCAGC 1 cut(s) 772
LweI GCATC 2 cut(s) 1164, 1369
MaeI CTAG 5 cut(s) 258, 293, 402, 813, 1082
MaeII ACGT 2 cut(s) 584, 1293
MaeIII GTNAC 5 cut(s) 118, 369, 701, 827, 932
MalI GATC 3 cut(s) 238, 1140, 1195
MboI GATC 3 cut(s) 236, 1138, 1193
MboII GAAGA 8 cut(s) 78, 311, 512, 547, 556, 633, 1103, 1274
MhlI GDGCHC 3 cut(s) 26, 1232, 1333
MluCI AATT 7 cut(s) 8, 124, 405, 459, 682, 879, 1148
MlyI GAGTC 2 cut(s) 226, 929
MmeI TCCRAC 2 cut(s) 54, 1066
Mph1103I ATGCAT 1 cut(s) 744
MroXI GAANNNNTTC 1 cut(s) 1011
MseI TTAA 7 cut(s) 273, 483, 578, 680, 798, 903, 1202
MspA1I CMGCKG 3 cut(s) 435, 1030, 1214
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 2 cut(s) 666, 790
MspR9I CCNGG 2 cut(s) 667, 1352
MvaI CCWGG 1 cut(s) 1352
MvnI CGCG 1 cut(s) 1030
MwoI GCNNNNNNNGC 4 cut(s) 113, 657, 760, 1328
NciI CCSGG 1 cut(s) 667
NcoI CCATGG 1 cut(s) 61
NdeII GATC 3 cut(s) 236, 1138, 1193
NlaIII CATG 8 cut(s) 65, 83, 349, 421, 512, 876, 1145, 1317
NlaIV GGNNCC 4 cut(s) 170, 431, 1063, 1268
NmeAIII GCCGAG 1 cut(s) 1395
NmuCI GTSAC 2 cut(s) 827, 932
NsiI ATGCAT 1 cut(s) 744
NspV TTCGAA 2 cut(s) 128, 860
PaeR7I CTCGAG 1 cut(s) 285
PagI TCATGA 2 cut(s) 79, 417
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PdmI GAANNNNTTC 1 cut(s) 1011
PfeI GAWTC 4 cut(s) 76, 595, 857, 1125
PinAI ACCGGT 1 cut(s) 789
PkrI GCNGC 1 cut(s) 762
PleI GAGTC 2 cut(s) 225, 929
PpsI GAGTC 2 cut(s) 225, 929
PsiI TTATAA 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 1350
PspGI CCWGG 1 cut(s) 1350
PspN4I GGNNCC 4 cut(s) 170, 431, 1063, 1268
PspPI GGNCC 2 cut(s) 512, 787
PspXI VCTCGAGB 1 cut(s) 285
PstI CTGCAG 2 cut(s) 901, 1338
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 3 cut(s) 590, 1001, 1108
RsaNI GTAC 3 cut(s) 589, 1000, 1107
SacII CCGCGG 1 cut(s) 1031
SaqAI TTAA 7 cut(s) 273, 483, 578, 680, 798, 903, 1202
SatI GCNGC 1 cut(s) 761
Sau3AI GATC 3 cut(s) 236, 1138, 1193
Sau96I GGNCC 2 cut(s) 512, 787
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 2 cut(s) 226, 929
ScrFI CCNGG 2 cut(s) 667, 1352
SduI GDGCHC 3 cut(s) 26, 1232, 1333
SfaNI GCATC 2 cut(s) 1164, 1369
SfcI CTRYAG 2 cut(s) 897, 1334
Sfr274I CTCGAG 1 cut(s) 285
Sfr303I CCGCGG 1 cut(s) 1031
SfuI TTCGAA 2 cut(s) 128, 860
SgrBI CCGCGG 1 cut(s) 1031
SinI GGWCC 1 cut(s) 787
SlaI CTCGAG 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 285, 482
SmoI CTYRAG 2 cut(s) 285, 482
SpeI ACTAGT 1 cut(s) 812
Sse9I AATT 7 cut(s) 8, 124, 405, 459, 682, 879, 1148
SsiI CCGC 6 cut(s) 188, 201, 410, 433, 1028, 1030
SspI AATATT 1 cut(s) 1187
SspMI CTAG 5 cut(s) 258, 293, 402, 813, 1082
StyD4I CCNGG 2 cut(s) 665, 1350
StyI CCWWGG 3 cut(s) 61, 693, 1270
TaaI ACNGT 2 cut(s) 450, 618
TaiI ACGT 2 cut(s) 587, 1296
TaqI TCGA 7 cut(s) 128, 152, 206, 286, 860, 985, 1224
TasI AATT 7 cut(s) 8, 124, 405, 459, 682, 879, 1148
TatI WGTACW 1 cut(s) 1106
TfiI GAWTC 4 cut(s) 76, 595, 857, 1125
Tru1I TTAA 7 cut(s) 273, 483, 578, 680, 798, 903, 1202
Tru9I TTAA 7 cut(s) 273, 483, 578, 680, 798, 903, 1202
TscAI CASTG 4 cut(s) 228, 623, 843, 850
TseFI GTSAC 2 cut(s) 827, 932
TseI GCWGC 1 cut(s) 760
Tsp45I GTSAC 2 cut(s) 827, 932
TspDTI ATGAA 2 cut(s) 312, 406
TspRI CASTG 4 cut(s) 228, 623, 843, 850
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 5 cut(s) 8, 124, 405, 682, 1148
XcmI CCANNNNNNNNNTGG 2 cut(s) 582, 946
XhoI CTCGAG 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 1011
XspI CTAG 5 cut(s) 258, 293, 402, 813, 1082
ZrmI AGTACT 1 cut(s) 1108
Zsp2I ATGCAT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.