pycom09g00390

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0011381
Physical Location & Seq
Forward (+)
327389 .. 329633
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGGGGGCAGTGACGTCATCAATGGCGGCCAAGTTCGCCTTTTTCCCGCCGAGCCCGCCGTCGTACGGGGTGGAGAAGGAGGAGGACACTGGGAAGCTGAAGATGACCGGGGTGGGGACGAGGGAAAACGTCGACGTCTTGAAGCTGCGGACCAAGAGAGGGAACGACGTCGTGGCGGTGTACATAAGGAATCCGTCGGCGAAGCTCACTGTTCTGCACTCGCATGGGAACGCAGCTGATCTGGGTCAGATGTACGAGTTGTTCAGTGAGCTGAGTCTTCACCTCCGAGTCAACTTGTTGGGGTATGATTATTCGGGGTACGGACAGTCTACTGGGAAGCCGAGTGAGCAAAACACTTATGCAGACATAGAAGCTGCATACAGATGCCTTGTGGAGAGGTACGATGCGAAGGAGGAGGATGTTATCTTATATGGGCAATCAGTTGGTAGTGGACCTACTCTCGATCTAGCAACCCGGTTACTGAAATTGAGGGCAGTGGTTCTTCATAGTCCGATCATGTCTGGTCTTCGGGTCATGTATCCAGTGAAGCGAACGTATTGGTTTGACATTTATAAGAATATCGACAAAATACCCTTCGTCAGTTGTCCTGTTCTGGTGATTCATGGGACGGCTGATGATGTTGTGGATTGGTCCCATGGCAAGCAGCTTTGGGATAATTGTAAAGAGAAGTATGAGCCGTTATGGATAAAAGGAGGGAATCATTGTGACTTGGAGCTCTACCCACAGTACATAAAGCATGTCAAAAAGTTTGTATTAACCATCGAGAAATATCCGCATCTGAGGAATAAATCAGGGCCAGTTACAGTTCAAATAGAAAATCCACGGAAAAGCACTGACACCAGGGAGAAATCTTGTTCCATCACAGATCACAAAGAGAATTCTAGACCCAGTATCGACCATAGAGAAAACCCCAGGCTAAGTACAGATCATAGAGAGAAGTCAAGGGCCAGCACAGATAAGAGAGAGAGAACAAGAAAAAGTATGGATCAACCTGAAAAAGCAAGTAATAGCGTGGAACAGCCAGAGAGAGCTCGGAATAGCATTGACCGCTTCGGAGAGATGTTCAGATCAGTTGGATTGTGCAATATTGATTGTTTTAAGCCCACAGCTACCAGCGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S9

Protein Analysis

381

Amino Acids

43.07

Weight (kDa)

8.85

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 67 - 174 1.2e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30380 AT3G30380
fragaria_vesca FvH4_6g46130 FvH4_6g46130
malus_domestica MD09G1076700.v1.1 MD17G1067200.v1.1
prunus_persica Prupe.3G247400_v2.0.a1 Prupe.3G247400_v2.0.a1
pyrus_communis pycom09g00390 pycom17g06750
rosa_chinensis RchiOBHm_Chr2g0164581
rosa_laevigata RLG00000021467
rosa_multiflora Rmu_co8187214.1_g000001
rosa_roxburghii Rroxscaffold_2G00086470
rosa_rugosa Rorug02G0509300
rosa_samantha Rh2AG576900 Rh2BG588000 Rh2CG558800 Rh2DG598600
rosa_wichuraiana Rw2G047900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AatII GACGTC 3 cut(s) 17, 138, 171
AccI GTMKAC 2 cut(s) 132, 329
AccII CGCG 1 cut(s) 1139
AciI CCGC 7 cut(s) 26, 47, 56, 148, 176, 794, 1069
AclWI GGATC 1 cut(s) 1014
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 898
AcuI CTGAAG 1 cut(s) 119
AcyI GRCGYC 3 cut(s) 14, 135, 168
AfaI GTAC 7 cut(s) 65, 182, 254, 320, 401, 749, 943
AfiI CCNNNNNNNGG 3 cut(s) 65, 114, 159
AgsI TTSAA 2 cut(s) 142, 830
AjnI CCWGG 2 cut(s) 860, 932
Alw21I GWGCWC 2 cut(s) 738, 1054
AlwI GGATC 1 cut(s) 1014
AoxI GGCC 3 cut(s) 27, 815, 966
ApeKI GCWGC 4 cut(s) 145, 233, 374, 664
ApoI RAATTY 1 cut(s) 898
AspLEI GCGC 1 cut(s) 1139
AspS9I GGNCC 5 cut(s) 150, 452, 651, 815, 966
AsuC2I CCSGG 2 cut(s) 109, 475
AsuHPI GGTGA 2 cut(s) 272, 628
AvaII GGWCC 3 cut(s) 150, 452, 651
BanII GRGCYC 3 cut(s) 56, 738, 1054
BbsI GAAGAC 2 cut(s) 269, 518
Bbv12I GWGCWC 2 cut(s) 738, 1054
BbvI GCAGC 4 cut(s) 132, 245, 361, 676
BccI CCATC 2 cut(s) 788, 887
BceAI ACGGC 3 cut(s) 43, 645, 682
BciT130I CCWGG 2 cut(s) 862, 934
BciVI GTATCC 1 cut(s) 549
BcnI CCSGG 2 cut(s) 109, 475
BfaI CTAG 2 cut(s) 467, 903
BfuI GTATCC 1 cut(s) 549
BisI GCNGC 5 cut(s) 27, 146, 234, 375, 665
BlsI GCNGC 5 cut(s) 28, 147, 235, 376, 666
Bme1390I CCNGG 4 cut(s) 109, 475, 862, 934
Bme18I GGWCC 3 cut(s) 150, 452, 651
BmgT120I GGNCC 5 cut(s) 150, 452, 651, 815, 966
BmiI GGNNCC 1 cut(s) 653
BmrFI CCNGG 4 cut(s) 109, 475, 862, 934
BmrI ACTGGG 3 cut(s) 99, 342, 903
BmsI GCATC 3 cut(s) 374, 394, 805
BmuI ACTGGG 3 cut(s) 99, 342, 903
BpiI GAAGAC 2 cut(s) 269, 518
BpuMI CCSGG 2 cut(s) 109, 475
BsaHI GRCGYC 3 cut(s) 14, 135, 168
BsaJI CCNNGG 5 cut(s) 108, 655, 842, 861, 932
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 114, 159
Bse1I ACTGG 5 cut(s) 94, 337, 542, 818, 909
BseBI CCWGG 2 cut(s) 862, 934
BseDI CCNNGG 5 cut(s) 108, 655, 842, 861, 932
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 3 cut(s) 65, 114, 159
BseMII CTCAG 2 cut(s) 263, 791
BseNI ACTGG 5 cut(s) 94, 337, 542, 818, 909
BseRI GAGGAG 2 cut(s) 95, 428
BseXI GCAGC 4 cut(s) 132, 245, 361, 676
BsgI GTGCAG 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 1139
BshFI GGCC 3 cut(s) 29, 817, 968
BsiHKAI GWGCWC 2 cut(s) 738, 1054
BsiSI CCGG 2 cut(s) 108, 475
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 3 cut(s) 130, 637, 640
BslI CCNNNNNNNGG 3 cut(s) 65, 114, 159
BsmFI GGGAC 3 cut(s) 130, 637, 640
BsnI GGCC 3 cut(s) 29, 817, 968
Bsp1286I GDGCHC 3 cut(s) 56, 738, 1054
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 7 cut(s) 26, 47, 56, 148, 176, 794, 1069
BspANI GGCC 3 cut(s) 29, 817, 968
BspCNI CTCAG 2 cut(s) 264, 792
BspFNI CGCG 1 cut(s) 1139
BspLI GGNNCC 1 cut(s) 653
BspPI GGATC 1 cut(s) 1014
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 5 cut(s) 94, 337, 542, 818, 909
BssECI CCNNGG 5 cut(s) 108, 655, 842, 861, 932
BssMI GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
BssNI GRCGYC 3 cut(s) 14, 135, 168
BssT1I CCWWGG 1 cut(s) 655
Bst2UI CCWGG 2 cut(s) 862, 934
Bst4CI ACNGT 4 cut(s) 211, 327, 747, 826
BstACI GRCGYC 3 cut(s) 14, 135, 168
BstAUI TGTACA 1 cut(s) 180
BstC8I GCNNGC 3 cut(s) 56, 662, 970
BstDEI CTNAG 3 cut(s) 272, 800, 938
BstDSI CCRYGG 2 cut(s) 655, 842
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 1 cut(s) 1139
BstHHI GCGC 1 cut(s) 1139
BstKTI GATC 7 cut(s) 241, 466, 516, 889, 949, 1009, 1091
BstMBI GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
BstMWI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 1068, 1136
BstNI CCWGG 2 cut(s) 862, 934
BstNSI RCATGY 1 cut(s) 761
BstSCI CCNGG 4 cut(s) 107, 473, 860, 932
BstUI CGCG 1 cut(s) 1139
BstV1I GCAGC 4 cut(s) 132, 245, 361, 676
BstV2I GAAGAC 2 cut(s) 269, 518
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 3 cut(s) 29, 817, 968
BtgI CCRYGG 2 cut(s) 655, 842
BtsCI GGATG 1 cut(s) 424
BtsI GCAGTG 2 cut(s) 15, 501
BtsIMutI CAGTG 7 cut(s) 15, 87, 207, 271, 501, 549, 852
Cac8I GCNNGC 3 cut(s) 56, 662, 970
CfoI GCGC 1 cut(s) 1139
Cfr13I GGNCC 5 cut(s) 150, 452, 651, 815, 966
Csp6I GTAC 7 cut(s) 64, 181, 253, 319, 400, 748, 942
CviAII CATG 6 cut(s) 224, 517, 535, 623, 656, 758
CviQI GTAC 7 cut(s) 64, 181, 253, 319, 400, 748, 942
DdeI CTNAG 3 cut(s) 272, 800, 938
DpnI GATC 7 cut(s) 240, 465, 515, 888, 948, 1008, 1090
DpnII GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
EaeI YGGCCR 1 cut(s) 27
Ecl136II GAGCTC 2 cut(s) 736, 1052
Eco130I CCWWGG 1 cut(s) 655
Eco24I GRGCYC 3 cut(s) 56, 738, 1054
Eco47I GGWCC 3 cut(s) 150, 452, 651
Eco53kI GAGCTC 2 cut(s) 736, 1052
Eco57I CTGAAG 1 cut(s) 119
EcoICRI GAGCTC 2 cut(s) 736, 1052
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 2 cut(s) 860, 932
EcoT14I CCWWGG 1 cut(s) 655
EcoT38I GRGCYC 3 cut(s) 56, 738, 1054
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 6 cut(s) 227, 520, 538, 626, 659, 761
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 3 cut(s) 130, 637, 640
FatI CATG 6 cut(s) 223, 516, 534, 622, 655, 757
FauI CCCGC 2 cut(s) 54, 63
FblI GTMKAC 2 cut(s) 132, 329
Fnu4HI GCNGC 5 cut(s) 27, 146, 234, 375, 665
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 3 cut(s) 56, 738, 1054
Fsp4HI GCNGC 5 cut(s) 27, 146, 234, 375, 665
FspBI CTAG 2 cut(s) 467, 903
GlaI GCGC 1 cut(s) 1138
GluI GCNGC 5 cut(s) 27, 146, 234, 375, 665
HaeIII GGCC 3 cut(s) 29, 817, 968
HapII CCGG 2 cut(s) 108, 475
HhaI GCGC 1 cut(s) 1139
Hin1I GRCGYC 3 cut(s) 14, 135, 168
Hin1II CATG 6 cut(s) 227, 520, 538, 626, 659, 761
Hin6I GCGC 1 cut(s) 1137
HinP1I GCGC 1 cut(s) 1137
HincII GTYRAC 2 cut(s) 133, 292
HindII GTYRAC 2 cut(s) 133, 292
HinfI GANTC 5 cut(s) 190, 274, 288, 619, 718
HpaII CCGG 2 cut(s) 108, 475
HphI GGTGA 2 cut(s) 272, 628
Hpy166II GTNNAC 5 cut(s) 133, 181, 292, 330, 452
Hpy188I TCNGA 7 cut(s) 249, 287, 513, 801, 1056, 1076, 1088
Hpy188III TCNNGA 4 cut(s) 139, 461, 784, 903
Hpy8I GTNNAC 5 cut(s) 133, 181, 292, 330, 452
Hpy99I CGWCG 6 cut(s) 64, 134, 137, 170, 173, 199
HpyAV CCTTC 3 cut(s) 70, 403, 604
HpyCH4III ACNGT 4 cut(s) 211, 327, 747, 826
HpyCH4IV ACGT 5 cut(s) 14, 129, 135, 168, 554
HpyCH4V TGCA 4 cut(s) 217, 362, 377, 1104
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 1068, 1136
HpyF3I CTNAG 3 cut(s) 272, 800, 938
HpySE526I ACGT 5 cut(s) 14, 129, 135, 168, 554
Hsp92I GRCGYC 3 cut(s) 14, 135, 168
Hsp92II CATG 6 cut(s) 227, 520, 538, 626, 659, 761
HspAI GCGC 1 cut(s) 1137
Kzo9I GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
LmnI GCTCC 1 cut(s) 733
Lsp1109I GCAGC 4 cut(s) 132, 245, 361, 676
LweI GCATC 3 cut(s) 374, 394, 805
MaeI CTAG 2 cut(s) 467, 903
MaeII ACGT 5 cut(s) 14, 129, 135, 168, 554
MaeIII GTNAC 4 cut(s) 10, 477, 725, 820
MalI GATC 7 cut(s) 240, 465, 515, 888, 948, 1008, 1090
MboI GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
MboII GAAGA 4 cut(s) 112, 269, 494, 518
MhlI GDGCHC 3 cut(s) 56, 738, 1054
MluCI AATT 3 cut(s) 485, 676, 898
MlyI GAGTC 2 cut(s) 283, 297
MmeI TCCRAC 1 cut(s) 1075
MseI TTAA 2 cut(s) 776, 1119
MslI CAYNNNNRTG 2 cut(s) 222, 382
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 2 cut(s) 108, 475
MspR9I CCNGG 4 cut(s) 109, 475, 862, 934
MvaI CCWGG 2 cut(s) 862, 934
MvnI CGCG 1 cut(s) 1139
MwoI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 1068, 1136
NciI CCSGG 2 cut(s) 109, 475
NcoI CCATGG 1 cut(s) 655
NdeII GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
NlaIII CATG 6 cut(s) 227, 520, 538, 626, 659, 761
NlaIV GGNNCC 1 cut(s) 653
NmeAIII GCCGAG 2 cut(s) 75, 366
NmuCI GTSAC 2 cut(s) 10, 725
NspI RCATGY 1 cut(s) 761
PfeI GAWTC 3 cut(s) 190, 619, 718
Pfl23II CGTACG 1 cut(s) 63
PkrI GCNGC 5 cut(s) 28, 147, 235, 376, 666
PleI GAGTC 2 cut(s) 282, 296
PpsI GAGTC 2 cut(s) 282, 296
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 738, 1054
Psp6I CCWGG 2 cut(s) 860, 932
PspGI CCWGG 2 cut(s) 860, 932
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 653
PspPI GGNCC 5 cut(s) 150, 452, 651, 815, 966
PsrI GAACNNNNNNTAC 2 cut(s) 245, 277
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 7 cut(s) 65, 182, 254, 320, 401, 749, 943
RsaNI GTAC 7 cut(s) 64, 181, 253, 319, 400, 748, 942
RseI CAYNNNNRTG 2 cut(s) 222, 382
SacI GAGCTC 2 cut(s) 738, 1054
SalI GTCGAC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 776, 1119
SatI GCNGC 5 cut(s) 27, 146, 234, 375, 665
Sau3AI GATC 7 cut(s) 238, 463, 513, 886, 946, 1006, 1088
Sau96I GGNCC 5 cut(s) 150, 452, 651, 815, 966
SchI GAGTC 2 cut(s) 283, 297
ScrFI CCNGG 4 cut(s) 109, 475, 862, 934
SduI GDGCHC 3 cut(s) 56, 738, 1054
SfaNI GCATC 3 cut(s) 374, 394, 805
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 3 cut(s) 150, 452, 651
SmiMI CAYNNNNRTG 2 cut(s) 222, 382
Sse9I AATT 3 cut(s) 485, 676, 898
SsiI CCGC 7 cut(s) 26, 47, 56, 148, 176, 794, 1069
SspI AATATT 1 cut(s) 1108
SspMI CTAG 2 cut(s) 467, 903
SstI GAGCTC 2 cut(s) 738, 1054
StyD4I CCNGG 4 cut(s) 107, 473, 860, 932
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 4 cut(s) 211, 327, 747, 826
TaiI ACGT 5 cut(s) 17, 132, 138, 171, 557
TaqI TCGA 5 cut(s) 132, 462, 582, 783, 915
TasI AATT 3 cut(s) 485, 676, 898
TatI WGTACW 3 cut(s) 180, 747, 941
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 190, 619, 718
Tru1I TTAA 2 cut(s) 776, 1119
Tru9I TTAA 2 cut(s) 776, 1119
TscAI CASTG 7 cut(s) 15, 94, 214, 271, 501, 549, 859
TseFI GTSAC 2 cut(s) 10, 725
TseI GCWGC 4 cut(s) 145, 233, 374, 664
Tsp45I GTSAC 2 cut(s) 10, 725
TspDTI ATGAA 2 cut(s) 494, 611
TspGWI ACGGA 3 cut(s) 183, 336, 859
TspRI CASTG 7 cut(s) 15, 94, 214, 271, 501, 549, 859
VpaK11BI GGWCC 3 cut(s) 150, 452, 651
XapI RAATTY 1 cut(s) 898
XbaI TCTAGA 1 cut(s) 902
XceI RCATGY 1 cut(s) 761
XmiI GTMKAC 2 cut(s) 132, 329
XspI CTAG 2 cut(s) 467, 903
ZraI GACGTC 3 cut(s) 15, 136, 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.