pycom09g01270

30S ribosomal protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012376
Physical Location & Seq
Forward (+)
955350 .. 956695
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01270.1

Sequence Viewer

Length: 570 bp
ATGTTATCTTTGGGAATGGGAATCGAGTGCCAGGCGAACCTAAACGCCGCCGCGTTGTGGCTGAAGCTGCCGCAGCAGAAGCTTCTTTTTGTCAACAGTGTTAATCAAAGAGCATTGAATTTCAAGCCGAAGGCTTCTTCTTTCTCTGTTTCTCACTCGCTGCCGGAACTGAGGCTCGGACTTACCCTCGCCGCAAGAAACGTAAGGCTCTCCGCCGCCGCAGAGTCTCTGGTCGCTGAAGAAACGGCCACCGATGACGAGGCCGCCGACACCGCCCCGGAAAAAGAGAAGCTGGGAGTGGTTGTGAAGCCATTTGAGAAACCGAGGCTCGTATTGAAGTTCATTTGGATGGAAAAGAACATCGGCATTGCACTCGACCAGATGATACCTGGTCACGGCTCCATCCCTCTTAGCCCCTATTACTTCTGGCCGAGGAAAGATGCGTGGGAGGAGCTCAAGGTCTTGCTTGAGAGCAAACCTTGGATTTCTCAGAAGCAGATGATTATCCTTCTCAATCAGGCTACCGATATCATCAATCTGTGGCAGCAGAGTGGCGGAAACTTGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PSRP-3_Ycf65

Protein Analysis

190

Amino Acids

21.03

Weight (kDa)

7.82

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSRP-3_Ycf65 PF04839 137 - 183 2.2e-26 Plastid and cyanobacterial ribosomal protein (PSRP-3 / Ycf65)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68590 AT1G68590
fragaria_vesca FvH4_6g45210
malus_domestica MD09G1087300.v1.1 MD17G1075600.v1.1 MD17G1076100.v1.1
prunus_persica Prupe.3G238100_v2.0.a1
pyrus_communis pycom09g01270 pycom17g07670
rosa_chinensis RchiOBHm_Chr2g0162921
rosa_laevigata RLG00000021361
rosa_multiflora Rmu_sc0006129.1_g000003
rosa_roxburghii Rroxscaffold_2G00087710
rosa_rugosa Rorug02G0499300
rosa_samantha Rh2AG565300 Rh2BG578000 Rh2CG547700 Rh2DG588200
rosa_wichuraiana Rw2G046790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 53
AcoI YGGCCR 2 cut(s) 246, 428
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 2 cut(s) 83, 258
AfiI CCNNNNNNNGG 2 cut(s) 57, 395
AgsI TTSAA 3 cut(s) 118, 124, 337
AjnI CCWGG 2 cut(s) 30, 388
AluBI AGCT 4 cut(s) 67, 82, 292, 454
AluI AGCT 4 cut(s) 67, 82, 292, 454
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 1 cut(s) 231
AoxI GGCC 3 cut(s) 246, 261, 428
ApeKI GCWGC 4 cut(s) 67, 73, 160, 544
ApoI RAATTY 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 278
BanII GRGCYC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 4 cut(s) 54, 85, 147, 556
BccI CCATC 2 cut(s) 343, 410
BceAI ACGGC 2 cut(s) 261, 412
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BciT130I CCWGG 2 cut(s) 32, 390
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 231
Bme1390I CCNGG 3 cut(s) 32, 278, 390
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 3 cut(s) 32, 278, 390
BmsI GCATC 1 cut(s) 430
BpuEI CTTGAG 2 cut(s) 440, 488
BpuMI CCSGG 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 503
BsaJI CCNNGG 4 cut(s) 276, 323, 431, 479
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 395
Bse3DI GCAATG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 503
BseBI CCWGG 2 cut(s) 32, 390
BseDI CCNNGG 4 cut(s) 276, 323, 431, 479
BseGI GGATG 2 cut(s) 354, 402
BseJI GATNNNNATC 1 cut(s) 503
BseLI CCNNNNNNNGG 2 cut(s) 57, 395
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 161, 503
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 4 cut(s) 54, 85, 147, 556
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 3 cut(s) 248, 263, 430
BsiHKAI GWGCWC 1 cut(s) 456
BsiSI CCGG 2 cut(s) 164, 278
BslI CCNNNNNNNGG 2 cut(s) 57, 395
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 3 cut(s) 248, 263, 430
Bsp1286I GDGCHC 1 cut(s) 456
BspANI GGCC 3 cut(s) 248, 263, 430
BspCNI CTCAG 2 cut(s) 162, 502
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 366
BssECI CCNNGG 4 cut(s) 276, 323, 431, 479
BssT1I CCWWGG 1 cut(s) 479
Bst2UI CCWGG 2 cut(s) 32, 390
Bst4CI ACNGT 1 cut(s) 98
BstDEI CTNAG 3 cut(s) 170, 410, 489
BstF5I GGATG 2 cut(s) 354, 402
BstFNI CGCG 1 cut(s) 53
BstMAI GTCTC 1 cut(s) 231
BstMWI GCNNNNNNNGC 4 cut(s) 67, 73, 79, 272
BstNI CCWGG 2 cut(s) 32, 390
BstSCI CCNGG 3 cut(s) 30, 276, 388
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 4 cut(s) 54, 85, 147, 556
BsuRI GGCC 3 cut(s) 248, 263, 430
BtsCI GGATG 2 cut(s) 354, 402
BtsIMutI CAGTG 1 cut(s) 103
CsiI ACCWGGT 1 cut(s) 388
DdeI CTNAG 3 cut(s) 170, 410, 489
EaeI YGGCCR 2 cut(s) 246, 428
EciI GGCGGA 2 cut(s) 202, 570
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 456
Eco32I GATATC 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 2 cut(s) 83, 258
EcoICRI GAGCTC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 30, 388
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 479
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 479
FokI GGATG 2 cut(s) 361, 389
FriOI GRGCYC 1 cut(s) 456
GsaI CCCAGC 1 cut(s) 296
HaeIII GGCC 3 cut(s) 248, 263, 430
HapII CCGG 2 cut(s) 164, 278
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 2 cut(s) 21, 224
HpaII CCGG 2 cut(s) 164, 278
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 179, 492
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 124, 518
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 371
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 73, 79, 272
HpyF3I CTNAG 3 cut(s) 170, 410, 489
HpySE526I ACGT 1 cut(s) 201
LmnI GCTCC 2 cut(s) 404, 451
Lsp1109I GCAGC 4 cut(s) 54, 85, 147, 556
LweI GCATC 1 cut(s) 430
MabI ACCWGGT 1 cut(s) 388
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 392
MboII GAAGA 2 cut(s) 129, 251
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 1 cut(s) 118
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 7 cut(s) 165, 197, 253, 318, 417, 426, 442
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 1 cut(s) 347
MspI CCGG 2 cut(s) 164, 278
MspR9I CCNGG 3 cut(s) 32, 278, 390
MvaI CCWGG 2 cut(s) 32, 390
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 4 cut(s) 67, 73, 79, 272
NciI CCSGG 1 cut(s) 278
NlaIV GGNNCC 1 cut(s) 400
NmeAIII GCCGAG 1 cut(s) 456
NmuCI GTSAC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 1 cut(s) 21
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 2 cut(s) 30, 388
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 2 cut(s) 30, 388
PspN4I GGNNCC 1 cut(s) 400
RseI CAYNNNNRTG 1 cut(s) 347
SacI GAGCTC 1 cut(s) 456
SaqAI TTAA 1 cut(s) 102
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 3 cut(s) 32, 278, 390
SduI GDGCHC 1 cut(s) 456
SetI ASST 9 cut(s) 42, 69, 84, 204, 294, 391, 456, 462, 481
SexAI ACCWGGT 1 cut(s) 388
SfaNI GCATC 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 2 cut(s) 455, 467
SmoI CTYRAG 2 cut(s) 455, 467
Sse9I AATT 1 cut(s) 118
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 3 cut(s) 30, 276, 388
StyI CCWWGG 1 cut(s) 479
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 24, 375
TasI AATT 1 cut(s) 118
TauI GCSGC 7 cut(s) 50, 53, 73, 194, 218, 221, 266
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 4 cut(s) 67, 73, 160, 544
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 331
TspRI CASTG 1 cut(s) 103
XapI RAATTY 1 cut(s) 118
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.