RLG00000021361

30S ribosomal protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012376
Physical Location & Seq
Reverse (-)
74516006 .. 74516961
956 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021361

Sequence Viewer

Length: 567 bp
ATGTTATCTATGGGTGTTGGGATCGAGTGTCAGGCAACCCTGAGTGCCTCCGCCGTCGTCGTCGTCAGGCCGAAGCTTCTGCCTCACCAAACACACAAGCTTTTCGTCAATAACAGCTCAACATTGAAGCCCAGGAGCACCTCTTCTTCCTTCTTCATTTCTCACTCGCTGCCGGACCTCAAGCTCAGACTCCCCCACAGAGACTTCAAACTCTCCGCCGCCGCCGAAGCTCTTGTCGCCGAAGAAACCGCCGCCGATGACGTCACCCCTGAAGAAGACAAAGAGAAGCTTGGAGTGGTTGTGAAGCCGATGGAGAAACCAAGGCTTGTGTTGAAGTTCATTTGGATGGAAAAGAACATAGGCATTGCACTTGACCAAATGATACCGGGGCATGGCTCCATCCCGCTCAGCCCGTATTACTTTTGGCCGAGGAAAGATGCTTGGGAGGAGCTCAAGGTCTTACTCGAGAGCAAGCCTTGGATATCTCAAAAGCAGATGATTATTCTTCTCAATCAGGCCACTGATATCATCAATTTGTGGCAGCAGAGTGGCGGCAATTTGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PSRP-3_Ycf65

Protein Analysis

189

Amino Acids

21.04

Weight (kDa)

7.86

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSRP-3_Ycf65 PF04839 136 - 182 2.1e-26 Plastid and cyanobacterial ribosomal protein (PSRP-3 / Ycf65)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68590 AT1G68590
fragaria_vesca FvH4_6g45210
malus_domestica MD09G1087300.v1.1 MD17G1075600.v1.1 MD17G1076100.v1.1
prunus_persica Prupe.3G238100_v2.0.a1
pyrus_communis pycom09g01270 pycom17g07670
rosa_chinensis RchiOBHm_Chr2g0162921
rosa_laevigata RLG00000021361
rosa_multiflora Rmu_sc0006129.1_g000003
rosa_roxburghii Rroxscaffold_2G00087710
rosa_rugosa Rorug02G0499300
rosa_samantha Rh2AG565300 Rh2BG578000 Rh2CG547700 Rh2DG588200
rosa_wichuraiana Rw2G046790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccBSI CCGCTC 1 cut(s) 406
AciI CCGC 8 cut(s) 51, 216, 219, 222, 249, 252, 404, 552
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 425
AcuI CTGAAG 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 261
AfiI CCNNNNNNNGG 1 cut(s) 392
AgsI TTSAA 3 cut(s) 127, 208, 334
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 7 cut(s) 76, 100, 117, 184, 230, 289, 451
AluI AGCT 7 cut(s) 76, 100, 117, 184, 230, 289, 451
Alw21I GWGCWC 2 cut(s) 140, 453
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 3 cut(s) 68, 425, 516
ApeKI GCWGC 2 cut(s) 169, 541
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
AspS9I GGNCC 1 cut(s) 175
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 2 cut(s) 77, 256
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 2 cut(s) 140, 453
BbvI GCAGC 2 cut(s) 156, 553
BccI CCATC 3 cut(s) 304, 340, 407
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 1 cut(s) 133
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 195
BisI GCNGC 6 cut(s) 170, 219, 222, 252, 542, 553
BlpI GCTNAGC 1 cut(s) 407
BlsI GCNGC 6 cut(s) 171, 220, 223, 253, 543, 554
Bme1390I CCNGG 2 cut(s) 133, 387
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 2 cut(s) 133, 387
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 407
BpuEI CTTGAG 2 cut(s) 164, 437
BpuMI CCSGG 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 5 cut(s) 131, 320, 386, 428, 476
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 363
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 5 cut(s) 131, 320, 386, 428, 476
BseGI GGATG 2 cut(s) 351, 399
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 3 cut(s) 32, 199, 421
BseRI GAGGAG 1 cut(s) 461
BseXI GCAGC 2 cut(s) 156, 553
BshFI GGCC 3 cut(s) 70, 427, 518
BsiHKAI GWGCWC 2 cut(s) 140, 453
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 2 cut(s) 173, 386
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 195
BsnI GGCC 3 cut(s) 70, 427, 518
BsoBI CYCGRG 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 140, 453
Bsp143I GATC 1 cut(s) 21
Bsp1720I GCTNAGC 1 cut(s) 407
BspACI CCGC 8 cut(s) 51, 216, 219, 222, 249, 252, 404, 552
BspANI GGCC 3 cut(s) 70, 427, 518
BspCNI CTCAG 3 cut(s) 33, 198, 420
BspLI GGNNCC 1 cut(s) 397
BspPI GGATC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 363
BssECI CCNNGG 5 cut(s) 131, 320, 386, 428, 476
BssMI GATC 1 cut(s) 21
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 2 cut(s) 320, 476
Bst2UI CCWGG 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 148
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 473
BstDEI CTNAG 3 cut(s) 41, 185, 407
BstF5I GGATG 2 cut(s) 351, 399
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 227, 236
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 2 cut(s) 131, 385
BstV1I GCAGC 2 cut(s) 156, 553
BstV2I GAAGAC 1 cut(s) 282
BsuRI GGCC 3 cut(s) 70, 427, 518
BtsCI GGATG 2 cut(s) 351, 399
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 175
CviAII CATG 1 cut(s) 392
DdeI CTNAG 3 cut(s) 41, 185, 407
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 425
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
EciI GGCGGA 2 cut(s) 40, 205
Ecl136II GAGCTC 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 320, 476
Eco24I GRGCYC 1 cut(s) 453
Eco32I GATATC 2 cut(s) 483, 526
Eco47I GGWCC 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 131
EcoRV GATATC 2 cut(s) 483, 526
EcoT14I CCWWGG 2 cut(s) 320, 476
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 2 cut(s) 320, 476
FaeI CATG 1 cut(s) 395
FaiI YATR 4 cut(s) 11, 359, 393, 565
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FatI CATG 1 cut(s) 391
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 6 cut(s) 170, 219, 222, 252, 542, 553
FokI GGATG 2 cut(s) 358, 386
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 6 cut(s) 170, 219, 222, 252, 542, 553
GluI GCNGC 6 cut(s) 170, 219, 222, 252, 542, 553
HaeIII GGCC 3 cut(s) 70, 427, 518
HapII CCGG 2 cut(s) 173, 386
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 1 cut(s) 395
HindIII AAGCTT 3 cut(s) 74, 98, 287
HinfI GANTC 1 cut(s) 189
HpaII CCGG 2 cut(s) 173, 386
HphI GGTGA 2 cut(s) 77, 256
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 1 cut(s) 466
Hpy99I CGWCG 3 cut(s) 59, 62, 65
HpyAV CCTTC 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 1 cut(s) 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 236
HpyF3I CTNAG 3 cut(s) 41, 185, 407
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 1 cut(s) 395
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 3 cut(s) 135, 401, 448
LpnPI CCDG 9 cut(s) 17, 52, 53, 118, 145, 186, 282, 399, 500
Lsp1109I GCAGC 2 cut(s) 156, 553
LweI GCATC 1 cut(s) 427
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 262
MalI GATC 1 cut(s) 23
MbiI CCGCTC 1 cut(s) 406
MboI GATC 1 cut(s) 21
MboII GAAGA 7 cut(s) 135, 138, 145, 254, 284, 287, 497
MhlI GDGCHC 2 cut(s) 140, 453
MluCI AATT 2 cut(s) 532, 556
MlyI GAGTC 1 cut(s) 183
MnlI CCTC 6 cut(s) 58, 93, 151, 188, 423, 439
MslI CAYNNNNRTG 1 cut(s) 344
MspI CCGG 2 cut(s) 173, 386
MspR9I CCNGG 2 cut(s) 133, 387
MvaI CCWGG 1 cut(s) 133
MwoI GCNNNNNNNGC 2 cut(s) 227, 236
NciI CCSGG 1 cut(s) 387
NdeII GATC 1 cut(s) 21
NlaIII CATG 1 cut(s) 395
NlaIV GGNNCC 1 cut(s) 397
NmeAIII GCCGAG 1 cut(s) 453
NmuCI GTSAC 1 cut(s) 262
PaeR7I CTCGAG 1 cut(s) 464
PkrI GCNGC 6 cut(s) 171, 220, 223, 253, 543, 554
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 344
SacI GAGCTC 1 cut(s) 453
SatI GCNGC 6 cut(s) 170, 219, 222, 252, 542, 553
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 2 cut(s) 133, 387
SduI GDGCHC 2 cut(s) 140, 453
SfaNI GCATC 1 cut(s) 427
Sfr274I CTCGAG 1 cut(s) 464
SinI GGWCC 1 cut(s) 175
SlaI CTCGAG 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 3 cut(s) 179, 452, 464
SmoI CTYRAG 3 cut(s) 179, 452, 464
Sse9I AATT 2 cut(s) 532, 556
SsiI CCGC 8 cut(s) 51, 216, 219, 222, 249, 252, 404, 552
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 2 cut(s) 131, 385
StyI CCWWGG 2 cut(s) 320, 476
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 24, 465
TasI AATT 2 cut(s) 532, 556
TauI GCSGC 4 cut(s) 221, 224, 254, 555
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 2 cut(s) 169, 541
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 145, 328
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 464
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.