pycom09g01900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012186
Physical Location & Seq
Forward (+)
1412525 .. 1414186
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01900.2

Sequence Viewer

Length: 777 bp
ATGTCGACTTCCATTCCCACCATCTCCGCTCCAACCCCCTCCATTCTCAGACTCAAAGTCGCTTCTTTTTCTCCCCCCAGAATCCGACCTCATATCCGAACAATCCCTTTTCGCAATTCACCCAAATTATGTTTGAACCCAATACAAGCTCAGAAATTTCCTGGGTTTTCCCGGAAAACAAATGGGTTTTTGTTTGATAGAAAGCTGAGGGTTTGTGCGAGTGCGAAAAATGAGGGTGTTGATGCAGCTGAGGAGGAGAGTAAAGGGGAGAGTACTATGCCAGAGCGGTTCAGGTACTTGACCAAGGAAGCTCCTGACCCTCCTGTTAGGTGGCCTTGGTTTGTTGCATTGGTGTTCCTTATCTATGCATGGAGAGCTGTCTTGCTGGAATTAGCTAACTGGAGAAAGGCTGCAGTGGGTGTTGTCCGTTTTGTGGGATACCTCTTGAAACTTATCCTGGCGCTAATCTTCCATTTCATAGGTGATCCCATTACATCCGTGATCAGATTCATAGAAACAACACTCTATGCCATACGCTCTTTCTACTCTGGCATAGTGGCATACGCTCCAATTCCAGAGTTGACAAGAGTATCGGATCATACTGGCATCCATTGTTCTTGCGATCGCAGAAGCTGTTGTTCCAAATGCCGTAAATTGTCAACCCTTTCTTGTCACAGTGTCTGGCGTAGTAGGCTATGCAGCAGTGGCAGGTTACATCTCGGAGCCTTTCTTCTGGACGATTCTAGTGGGGTTCTACGGTTACTCACGATTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.04

Weight (kDa)

9.97

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28210
fragaria_vesca FvH4_6g44760
malus_domestica MD09G1093800.v1.1 MD17G1083500.v1.1
prunus_persica Prupe.3G231600_v2.0.a1
pyrus_communis pycom09g01900 pycom17g08130
rosa_chinensis RchiOBHm_Chr2g0161911
rosa_laevigata RLG00000021306
rosa_multiflora Rmu_sc0008565.1_g000006 Rmu_sc0008565.1_g000007 Rmu_sc0018381.1_g000007
rosa_roxburghii Rroxscaffold_2G00088470
rosa_rugosa Rorug02G0493100
rosa_samantha Rh2AG559000 Rh2BG572300 Rh2CG542700 Rh2DG582200
rosa_wichuraiana Rw2G046270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 699
AccBSI CCGCTC 2 cut(s) 29, 286
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 27, 286
AclWI GGATC 2 cut(s) 479, 603
AcsI RAATTY 1 cut(s) 155
AfaI GTAC 2 cut(s) 274, 296
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 2 cut(s) 136, 448
AhdI GACNNNNNGTC 1 cut(s) 56
AjnI CCWGG 2 cut(s) 160, 456
AluBI AGCT 7 cut(s) 149, 205, 248, 311, 377, 395, 633
AluI AGCT 7 cut(s) 149, 205, 248, 311, 377, 395, 633
AlwI GGATC 2 cut(s) 479, 603
AlwNI CAGNNNCTG 2 cut(s) 633, 681
AoxI GGCC 1 cut(s) 332
ApeKI GCWGC 3 cut(s) 245, 410, 699
ApoI RAATTY 1 cut(s) 155
AsiSI GCGATCGC 1 cut(s) 625
AspLEI GCGC 1 cut(s) 463
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 111, 494
BaeI ACNNNNGTAYC 2 cut(s) 573, 606
BbvCI CCTCAGC 2 cut(s) 206, 249
BbvI GCAGC 3 cut(s) 257, 397, 711
BccI CCATC 1 cut(s) 29
BceAI ACGGC 1 cut(s) 633
BciT130I CCWGG 2 cut(s) 162, 458
BciVI GTATCC 1 cut(s) 431
BclI TGATCA 1 cut(s) 501
BcnI CCSGG 1 cut(s) 172
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 464
BfuAI ACCTGC 1 cut(s) 699
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 3 cut(s) 246, 411, 700
BlsI GCNGC 3 cut(s) 247, 412, 701
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 3 cut(s) 162, 172, 458
BmeRI GACNNNNNGTC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 724
BmrFI CCNGG 3 cut(s) 162, 172, 458
BmsI GCATC 2 cut(s) 232, 615
BpmI CTGGAG 1 cut(s) 421
Bpu10I CCTNAGC 2 cut(s) 206, 249
BpuMI CCSGG 1 cut(s) 172
BsaJI CCNNGG 3 cut(s) 161, 303, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 404, 607
BseBI CCWGG 2 cut(s) 162, 458
BseDI CCNNGG 3 cut(s) 161, 303, 335
BseGI GGATG 2 cut(s) 494, 606
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 4 cut(s) 61, 164, 197, 240
BseNI ACTGG 2 cut(s) 404, 607
BseRI GAGGAG 2 cut(s) 266, 269
BseXI GCAGC 3 cut(s) 257, 397, 711
Bsh1285I CGRYCG 1 cut(s) 625
BshFI GGCC 1 cut(s) 334
BsiEI CGRYCG 1 cut(s) 625
BsiSI CCGG 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 433
BsnI GGCC 1 cut(s) 334
Bsp143I GATC 4 cut(s) 484, 501, 595, 622
BspACI CCGC 2 cut(s) 27, 286
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 4 cut(s) 60, 163, 198, 241
BspLI GGNNCC 1 cut(s) 724
BspMAI CTGCAG 1 cut(s) 415
BspMI ACCTGC 1 cut(s) 699
BspPI GGATC 2 cut(s) 479, 603
BsrBI CCGCTC 2 cut(s) 29, 286
BsrI ACTGG 2 cut(s) 404, 607
BssECI CCNNGG 3 cut(s) 161, 303, 335
BssMI GATC 4 cut(s) 484, 501, 595, 622
BssT1I CCWWGG 2 cut(s) 303, 335
Bst2UI CCWGG 2 cut(s) 162, 458
Bst4CI ACNGT 2 cut(s) 677, 759
BstDEI CTNAG 4 cut(s) 47, 150, 206, 249
BstF5I GGATG 2 cut(s) 494, 606
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 4 cut(s) 487, 504, 598, 625
BstMBI GATC 4 cut(s) 484, 501, 595, 622
BstMCI CGRYCG 1 cut(s) 625
BstMWI GCNNNNNNNGC 3 cut(s) 374, 691, 705
BstNI CCWGG 2 cut(s) 162, 458
BstSCI CCNGG 3 cut(s) 160, 170, 456
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 257, 397, 711
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 1 cut(s) 334
BtsCI GGATG 2 cut(s) 494, 606
BtsI GCAGTG 2 cut(s) 420, 709
BtsIMutI CAGTG 3 cut(s) 420, 682, 709
BveI ACCTGC 1 cut(s) 699
CaiI CAGNNNCTG 2 cut(s) 633, 681
CfoI GCGC 1 cut(s) 463
Csp6I GTAC 2 cut(s) 273, 295
CviAII CATG 1 cut(s) 369
CviQI GTAC 2 cut(s) 273, 295
DdeI CTNAG 4 cut(s) 47, 150, 206, 249
DpnI GATC 4 cut(s) 486, 503, 597, 624
DpnII GATC 4 cut(s) 484, 501, 595, 622
DriI GACNNNNNGTC 1 cut(s) 56
Eam1105I GACNNNNNGTC 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 303, 335
EcoRII CCWGG 2 cut(s) 160, 456
EcoT14I CCWWGG 2 cut(s) 303, 335
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 2 cut(s) 303, 335
FaeI CATG 1 cut(s) 372
FatI CATG 1 cut(s) 368
FbaI TGATCA 1 cut(s) 501
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 246, 411, 700
FokI GGATG 2 cut(s) 481, 593
Fsp4HI GCNGC 3 cut(s) 246, 411, 700
FspBI CTAG 1 cut(s) 744
GlaI GCGC 1 cut(s) 462
GluI GCNGC 3 cut(s) 246, 411, 700
GsuI CTGGAG 1 cut(s) 421
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 1 cut(s) 334
HapII CCGG 1 cut(s) 172
HhaI GCGC 1 cut(s) 463
Hin1II CATG 1 cut(s) 372
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HincII GTYRAC 3 cut(s) 6, 582, 660
HindII GTYRAC 3 cut(s) 6, 582, 660
HinfI GANTC 4 cut(s) 51, 81, 507, 740
HpaII CCGG 1 cut(s) 172
HphI GGTGA 2 cut(s) 111, 494
Hpy166II GTNNAC 3 cut(s) 6, 582, 660
Hpy188I TCNGA 7 cut(s) 50, 86, 98, 153, 506, 595, 722
Hpy188III TCNNGA 5 cut(s) 314, 445, 575, 734, 766
Hpy8I GTNNAC 3 cut(s) 6, 582, 660
HpyCH4III ACNGT 2 cut(s) 677, 759
HpyCH4V TGCA 5 cut(s) 245, 347, 368, 413, 699
HpyF10VI GCNNNNNNNGC 3 cut(s) 374, 691, 705
HpyF3I CTNAG 4 cut(s) 47, 150, 206, 249
Hsp92II CATG 1 cut(s) 372
HspAI GCGC 1 cut(s) 461
Ksp22I TGATCA 1 cut(s) 501
Kzo9I GATC 4 cut(s) 484, 501, 595, 622
LmnI GCTCC 4 cut(s) 34, 316, 571, 722
Lsp1109I GCAGC 3 cut(s) 257, 397, 711
LweI GCATC 2 cut(s) 232, 615
MaeI CTAG 1 cut(s) 744
MaeIII GTNAC 3 cut(s) 671, 711, 759
MalI GATC 4 cut(s) 486, 503, 597, 624
MbiI CCGCTC 2 cut(s) 29, 286
MboI GATC 4 cut(s) 484, 501, 595, 622
MboII GAAGA 2 cut(s) 460, 722
MluCI AATT 6 cut(s) 115, 125, 155, 389, 570, 653
MlyI GAGTC 1 cut(s) 45
MmeI TCCRAC 2 cut(s) 56, 109
MnlI CCTC 8 cut(s) 49, 99, 201, 226, 244, 247, 330, 452
Mph1103I ATGCAT 1 cut(s) 370
MseI TTAA 1 cut(s) 775
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 3 cut(s) 162, 172, 458
MvaI CCWGG 2 cut(s) 162, 458
MwoI GCNNNNNNNGC 3 cut(s) 374, 691, 705
NciI CCSGG 1 cut(s) 172
NdeII GATC 4 cut(s) 484, 501, 595, 622
NlaIII CATG 1 cut(s) 372
NlaIV GGNNCC 1 cut(s) 724
NmuCI GTSAC 1 cut(s) 671
NsiI ATGCAT 1 cut(s) 370
PfeI GAWTC 3 cut(s) 81, 507, 740
PfoI TCCNGGA 1 cut(s) 170
PkrI GCNGC 3 cut(s) 247, 412, 701
Ple19I CGATCG 1 cut(s) 625
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
Psp6I CCWGG 2 cut(s) 160, 456
PspGI CCWGG 2 cut(s) 160, 456
PspN4I GGNNCC 1 cut(s) 724
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 2 cut(s) 633, 681
PvuI CGATCG 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 248
RgaI GCGATCGC 1 cut(s) 625
RsaI GTAC 2 cut(s) 274, 296
RsaNI GTAC 2 cut(s) 273, 295
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 775
SatI GCNGC 3 cut(s) 246, 411, 700
Sau3AI GATC 4 cut(s) 484, 501, 595, 622
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 3 cut(s) 162, 172, 458
SfaAI GCGATCGC 1 cut(s) 625
SfaNI GCATC 2 cut(s) 232, 615
SfcI CTRYAG 1 cut(s) 411
SgfI GCGATCGC 1 cut(s) 625
Sse9I AATT 6 cut(s) 115, 125, 155, 389, 570, 653
SsiI CCGC 2 cut(s) 27, 286
SspMI CTAG 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 160, 170, 456
StyI CCWWGG 2 cut(s) 303, 335
TaaI ACNGT 2 cut(s) 677, 759
TaqI TCGA 1 cut(s) 5
TasI AATT 6 cut(s) 115, 125, 155, 389, 570, 653
TatI WGTACW 1 cut(s) 272
TfiI GAWTC 3 cut(s) 81, 507, 740
Tru1I TTAA 1 cut(s) 775
Tru9I TTAA 1 cut(s) 775
TscAI CASTG 3 cut(s) 420, 682, 709
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 3 cut(s) 245, 410, 699
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 2 cut(s) 466, 499
TspGWI ACGGA 2 cut(s) 416, 487
TspRI CASTG 3 cut(s) 420, 682, 709
XapI RAATTY 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.