RLG00000021306

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012186
Physical Location & Seq
Reverse (-)
74025969 .. 74028143
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021306

Sequence Viewer

Length: 1041 bp
ATGGCGACCACCATTCCCATCCTCACCTCTCCAAACCCTTCTTTTCTCAGACCCAAAATCACTTCTGTCTCTCCTCTCACCACTCGACTTCATAACCAAACCATTCCCTTTCGCTCTTCAACCAAAATCCAATCTTTGAACACAAACCAAACCCAGAATTTTCCTGGGTTTTCAGGGAAAGCAATTGGGTTTTTGTTTGAAAGGAAGTTAAGGGTTCGTGTGAGTGCAAAAGAGGATGGCGTTGATGAAGCTGAGGAGGAAAGTGGAGGGGAGAGTACTATGCCGGAGCGGTTTAGGTACTTGACCAAAGAAGCTCCTGACCCTCCTGTGAGGTGGCCTTGGTTTGTGGCATTGGCCTTCCTCATTTATGCATGGAGAGCTGTCTTGTTAGAGTTATCTAACTGGAAAAAGGGTGCACTAGCTGTTGTCCAATTTGTGGGATACCTCTTGAAGCTTAGCTTGGCCCTTATCTTCCATTTCATAGGTGATCCTATCACTTACACGATCAGATTCTTAGAAACAACTATCTATGCTGTTCGCGCTTACTACTCTGGCATAGTGGCATACGCTCCAATTCGAGAGTTGACTACAGTGATCATGCTGGCTTCAGCTGTTCTTGCTATTGCAGAAGCTGTCGTTCCAAACTCAGCAAACTGCCAACCTATTCTGCTCACGTTATCTGGAGTAGTAGGGTATGCAGTTGTGTTTAATTACATCTCAGAGCCTTTCTTCTACACGATTCTAGTGGGGTTGTATGGTTACTCACGATTTGTTAAAAAGAGGGATGACGTTACATCTGCATTGCCTTCTGCGGCTGTGCTGGCTGCTGTTGGAGAACCGTGGATTAGAGTTTTGGTTATACCATTGTTTCTGGCATTGGCCATTTATAACCATTCAAAGAAGCTTCCTGAAAGAACGGAAGATGTTGCAGTGACCAACAGGAAGCTTCCATTGCCCTTGTTGGGTGCAGCCTTAGCTATTGGAATTCGGCTTGCTGCTAAGTGGGCTGGGTACCGGCATCTAACATGGAAGATAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

347

Amino Acids

38.45

Weight (kDa)

9.68

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AtTam38 PF25114 81 - 346 3.8e-136 AtTam38 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28210
fragaria_vesca FvH4_6g44760
malus_domestica MD09G1093800.v1.1 MD17G1083500.v1.1
prunus_persica Prupe.3G231600_v2.0.a1
pyrus_communis pycom09g01900 pycom17g08130
rosa_chinensis RchiOBHm_Chr2g0161911
rosa_laevigata RLG00000021306
rosa_multiflora Rmu_sc0008565.1_g000006 Rmu_sc0008565.1_g000007 Rmu_sc0018381.1_g000007
rosa_roxburghii Rroxscaffold_2G00088470
rosa_rugosa Rorug02G0493100
rosa_samantha Rh2AG559000 Rh2BG572300 Rh2CG542700 Rh2DG582200
rosa_wichuraiana Rw2G046270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 888
Acc65I GGTACC 1 cut(s) 1011
AccB1I GGYRCC 1 cut(s) 1011
AccB7I CCANNNNNTGG 1 cut(s) 436
AccBSI CCGCTC 1 cut(s) 289
AccII CGCG 1 cut(s) 540
AciI CCGC 2 cut(s) 289, 812
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 879
AcsI RAATTY 2 cut(s) 157, 984
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 3 cut(s) 277, 299, 1013
AfiI CCNNNNNNNGG 2 cut(s) 436, 962
AgsI TTSAA 5 cut(s) 120, 139, 200, 451, 897
AjnI CCWGG 1 cut(s) 163
AjuI GAANNNNNNNTTGG 2 cut(s) 443, 475
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 1 cut(s) 73
Alw44I GTGCAC 1 cut(s) 414
AlwI GGATC 1 cut(s) 482
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 4 cut(s) 335, 354, 462, 879
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 3 cut(s) 824, 968, 995
ApoI RAATTY 2 cut(s) 157, 984
Asp718I GGTACC 1 cut(s) 1011
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 463
AsuHPI GGTGA 3 cut(s) 16, 70, 497
BaeGI GKGCMC 1 cut(s) 418
BalI TGGCCA 1 cut(s) 881
BanI GGYRCC 1 cut(s) 1011
Bbv12I GWGCWC 1 cut(s) 418
BbvCI CCTCAGC 1 cut(s) 252
BbvI GCAGC 3 cut(s) 811, 980, 982
BccI CCATC 2 cut(s) 26, 230
BciT130I CCWGG 1 cut(s) 165
BciVI GTATCC 1 cut(s) 434
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 2 cut(s) 419, 743
BfmI CTRYAG 1 cut(s) 588
BfuI GTATCC 1 cut(s) 434
BisI GCNGC 4 cut(s) 813, 825, 969, 996
BlpI GCTNAGC 1 cut(s) 455
BlsI GCNGC 4 cut(s) 814, 826, 970, 997
BmcAI AGTACT 1 cut(s) 277
Bme1390I CCNGG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 1 cut(s) 1013
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 1 cut(s) 1027
BpmI CTGGAG 1 cut(s) 702
Bpu10I CCTNAGC 2 cut(s) 252, 973
Bpu1102I GCTNAGC 1 cut(s) 455
BsaJI CCNNGG 3 cut(s) 164, 338, 839
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 962
Bse118I RCCGGY 1 cut(s) 1014
Bse1I ACTGG 1 cut(s) 407
Bse3DI GCAATG 2 cut(s) 800, 950
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 164, 338, 839
BseGI GGATG 3 cut(s) 18, 241, 790
BseLI CCNNNNNNNGG 2 cut(s) 436, 962
BseMI GCAATG 2 cut(s) 800, 950
BseMII CTCAG 4 cut(s) 61, 243, 660, 732
BseNI ACTGG 1 cut(s) 407
BseRI GAGGAG 2 cut(s) 63, 269
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 3 cut(s) 811, 980, 982
BseYI CCCAGC 1 cut(s) 1007
BsgI GTGCAG 1 cut(s) 987
Bsh1236I CGCG 1 cut(s) 540
BshFI GGCC 4 cut(s) 337, 356, 464, 881
BshNI GGYRCC 1 cut(s) 1011
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 2 cut(s) 284, 1015
BslI CCNNNNNNNGG 2 cut(s) 436, 962
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 4 cut(s) 337, 356, 464, 881
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 3 cut(s) 487, 504, 594
Bsp1720I GCTNAGC 1 cut(s) 455
BspACI CCGC 2 cut(s) 289, 812
BspANI GGCC 4 cut(s) 337, 356, 464, 881
BspCNI CTCAG 4 cut(s) 60, 244, 659, 731
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 1013
BspPI GGATC 1 cut(s) 482
BspQI GCTCTTC 1 cut(s) 121
BspT107I GGYRCC 1 cut(s) 1011
BsrBI CCGCTC 1 cut(s) 289
BsrDI GCAATG 2 cut(s) 800, 950
BsrFI RCCGGY 1 cut(s) 1014
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 1014
BssECI CCNNGG 3 cut(s) 164, 338, 839
BssMI GATC 3 cut(s) 487, 504, 594
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 592, 840
Bst6I CTCTTC 1 cut(s) 121
BstC8I GCNNGC 3 cut(s) 603, 822, 993
BstDEI CTNAG 8 cut(s) 47, 252, 455, 514, 646, 718, 973, 999
BstDSI CCRYGG 1 cut(s) 839
BstF5I GGATG 3 cut(s) 18, 241, 790
BstFNI CGCG 1 cut(s) 540
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 3 cut(s) 490, 507, 597
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 3 cut(s) 487, 504, 594
BstMWI GCNNNNNNNGC 7 cut(s) 377, 539, 617, 821, 952, 974, 1004
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 1 cut(s) 588
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 3 cut(s) 811, 980, 982
BsuI GTATCC 1 cut(s) 434
BsuRI GGCC 4 cut(s) 337, 356, 464, 881
BtgI CCRYGG 1 cut(s) 839
BtsCI GGATG 3 cut(s) 18, 241, 790
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 2 cut(s) 597, 936
Cac8I GCNNGC 3 cut(s) 603, 822, 993
CaiI CAGNNNCTG 1 cut(s) 632
CfoI GCGC 1 cut(s) 542
Cfr10I RCCGGY 1 cut(s) 1014
Cfr13I GGNCC 1 cut(s) 463
Csp6I GTAC 3 cut(s) 276, 298, 1012
CviAII CATG 3 cut(s) 372, 598, 1026
CviQI GTAC 3 cut(s) 276, 298, 1012
DdeI CTNAG 8 cut(s) 47, 252, 455, 514, 646, 718, 973, 999
DpnI GATC 3 cut(s) 489, 506, 596
DpnII GATC 3 cut(s) 487, 504, 594
EaeI YGGCCR 1 cut(s) 879
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 591
EcoRI GAATTC 1 cut(s) 984
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 3 cut(s) 375, 601, 1029
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 3 cut(s) 371, 597, 1025
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 4 cut(s) 813, 825, 969, 996
FokI GGATG 3 cut(s) 5, 248, 797
Fsp4HI GCNGC 4 cut(s) 813, 825, 969, 996
FspBI CTAG 2 cut(s) 419, 743
GlaI GCGC 1 cut(s) 541
GluI GCNGC 4 cut(s) 813, 825, 969, 996
GsaI CCCAGC 1 cut(s) 1011
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 4 cut(s) 337, 356, 464, 881
HapII CCGG 2 cut(s) 284, 1015
HhaI GCGC 1 cut(s) 542
Hin1II CATG 3 cut(s) 375, 601, 1029
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HindIII AAGCTT 3 cut(s) 452, 902, 944
HinfI GANTC 2 cut(s) 510, 739
HpaII CCGG 2 cut(s) 284, 1015
HphI GGTGA 3 cut(s) 16, 70, 497
Hpy166II GTNNAC 2 cut(s) 416, 585
Hpy188I TCNGA 3 cut(s) 50, 509, 721
Hpy188III TCNNGA 6 cut(s) 317, 448, 578, 681, 765, 908
Hpy8I GTNNAC 2 cut(s) 416, 585
HpyAV CCTTC 3 cut(s) 48, 367, 816
HpyCH4III ACNGT 2 cut(s) 592, 840
HpyCH4IV ACGT 2 cut(s) 674, 789
HpyCH4V TGCA 8 cut(s) 227, 371, 416, 626, 698, 800, 929, 968
HpyF10VI GCNNNNNNNGC 7 cut(s) 377, 539, 617, 821, 952, 974, 1004
HpyF3I CTNAG 8 cut(s) 47, 252, 455, 514, 646, 718, 973, 999
HpySE526I ACGT 2 cut(s) 674, 789
Hsp92II CATG 3 cut(s) 375, 601, 1029
HspAI GCGC 1 cut(s) 540
KpnI GGTACC 1 cut(s) 1015
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 3 cut(s) 487, 504, 594
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 3 cut(s) 286, 319, 574
Lsp1109I GCAGC 3 cut(s) 811, 980, 982
LweI GCATC 1 cut(s) 1027
MaeI CTAG 2 cut(s) 419, 743
MaeII ACGT 2 cut(s) 674, 789
MaeIII GTNAC 3 cut(s) 758, 790, 931
MalI GATC 3 cut(s) 489, 506, 596
MbiI CCGCTC 1 cut(s) 289
MboI GATC 3 cut(s) 487, 504, 594
MboII GAAGA 4 cut(s) 108, 463, 721, 932
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 881
MluCI AATT 6 cut(s) 157, 183, 431, 573, 709, 984
MluNI TGGCCA 1 cut(s) 881
MmeI TCCRAC 1 cut(s) 811
Mox20I TGGCCA 1 cut(s) 881
Mph1103I ATGCAT 1 cut(s) 373
MscI TGGCCA 1 cut(s) 881
MseI TTAA 3 cut(s) 209, 708, 774
Msp20I TGGCCA 1 cut(s) 881
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 2 cut(s) 284, 1015
MspR9I CCNGG 1 cut(s) 165
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 7 cut(s) 377, 539, 617, 821, 952, 974, 1004
NdeII GATC 3 cut(s) 487, 504, 594
NlaIII CATG 3 cut(s) 375, 601, 1029
NlaIV GGNNCC 1 cut(s) 1013
NmuCI GTSAC 1 cut(s) 931
NsiI ATGCAT 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 121
PfeI GAWTC 2 cut(s) 510, 739
PflMI CCANNNNNTGG 1 cut(s) 436
PkrI GCNGC 4 cut(s) 814, 826, 970, 997
PsiI TTATAA 1 cut(s) 888
Psp6I CCWGG 1 cut(s) 163
PspFI CCCAGC 1 cut(s) 1007
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 1013
PspPI GGNCC 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 632
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 3 cut(s) 277, 299, 1013
RsaNI GTAC 3 cut(s) 276, 298, 1012
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 3 cut(s) 209, 708, 774
SatI GCNGC 4 cut(s) 813, 825, 969, 996
Sau3AI GATC 3 cut(s) 487, 504, 594
Sau96I GGNCC 1 cut(s) 463
ScaI AGTACT 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 1 cut(s) 1027
SfcI CTRYAG 1 cut(s) 588
Sse9I AATT 6 cut(s) 157, 183, 431, 573, 709, 984
SsiI CCGC 2 cut(s) 289, 812
SspMI CTAG 2 cut(s) 419, 743
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 2 cut(s) 592, 840
TaiI ACGT 2 cut(s) 677, 792
TaqI TCGA 2 cut(s) 85, 577
TasI AATT 6 cut(s) 157, 183, 431, 573, 709, 984
TatI WGTACW 1 cut(s) 275
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 2 cut(s) 510, 739
Tru1I TTAA 3 cut(s) 209, 708, 774
Tru9I TTAA 3 cut(s) 209, 708, 774
TscAI CASTG 2 cut(s) 597, 936
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 3 cut(s) 824, 968, 995
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 3 cut(s) 80, 261, 469
TspGWI ACGGA 1 cut(s) 932
TspRI CASTG 2 cut(s) 597, 936
Van91I CCANNNNNTGG 1 cut(s) 436
VneI GTGCAC 1 cut(s) 414
XapI RAATTY 2 cut(s) 157, 984
XcmI CCANNNNNNNNNTGG 1 cut(s) 161
XspI CTAG 2 cut(s) 419, 743
ZrmI AGTACT 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.