pycom09g02250

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0014776
Physical Location & Seq
Reverse (-)
1728036 .. 1729845
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02250.4

Sequence Viewer

Length: 759 bp
ATGCAATTAGCATCCGAGCTTGGTCAGCTTACAATGGGGTGTCCACTTGCAGAGTTTGAAAGATTTATCCACTGCGCTGCTCCTGTTATTGCTTCTTCATATGTGCATAAAAAATGTGGTTCAATGCCATTATGTGCCCATTTTGTTCCTTACTTTCCCGCTGTTCAATTGTTTCGACCCCATAACCATGGTTCCAAGACATTATATTTCACACATTCTTCAAAAGCAGCAGAGTTGAACCATCTTCAGGCAAGGACAGAGAAACATGACGGAAGTGGCCTGTCCAGTCTAGAATGTTCACCTGATTTTGAGCTCATATTTGAATTTTTTTTTGAATCCGAGACAATTCTGGAGCTAACCGACGTTGAAACTTCTATCATCACATGTTTGTACCCTATGTATCGAATTCCAGAAGGGAACTTGCGTGCAACATTCTTAACTTACCATTCACTTGGACATTTTGTACGGAGACCTATTTCGGTAGATCTTGCCAATAAGTACACAAGTCACCGCCCTGTGTTAGGATTGCAAAGCCACAATGCACATGGTGAATGCTGGTTTGATCCCAAAACGCCTTTCGGACATTCTTCAAATGAATCTACTTCTAACAGTGCAGAAATTCTCAAAGAAAGGCTCAGAACATTGGAGGATGATGCATTAAGTTTTGCAAGAGTTTGCGTTTGCAAGGATCAAGTCATGGTCTCCAATAGGCAGCCGGATTACGAGTTCTTCATTTCTCGAAGAACACATCATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

253

Amino Acids

28.67

Weight (kDa)

6.45

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014776)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44320
malus_domestica MD17G1087100.v1.1
prunus_persica Prupe.3G227600_v2.0.a1 Prupe.3G227600_v2.0.a1
pyrus_communis pycom09g02250 pycom17g08430
rosa_chinensis RchiOBHm_Chr2g0161201
rosa_laevigata RLG00000021267
rosa_multiflora Rmu_sc0006420.1_g000047
rosa_roxburghii Rroxscaffold_2G00089030
rosa_rugosa Rorug02G0487000 Rorug02G0487100
rosa_samantha Rh2AG553800 Rh2BG567300 Rh2CG537600 Rh2DG576700
rosa_wichuraiana Rw2G045810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 159, 511
AclWI GGATC 2 cut(s) 557, 696
AcsI RAATTY 3 cut(s) 323, 405, 618
AcuI CTGAAG 1 cut(s) 230
AdeI CACNNNGTG 1 cut(s) 548
AfaI GTAC 3 cut(s) 392, 465, 500
AfiI CCNNNNNNNGG 2 cut(s) 247, 521
AflIII ACRYGT 1 cut(s) 383
AgsI TTSAA 9 cut(s) 59, 123, 167, 222, 238, 323, 335, 368, 591
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AloI GAACNNNNNNTCC 2 cut(s) 710, 742
AluBI AGCT 4 cut(s) 19, 28, 313, 355
AluI AGCT 4 cut(s) 19, 28, 313, 355
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 3 cut(s) 335, 463, 706
AlwI GGATC 2 cut(s) 557, 696
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 3 cut(s) 77, 227, 712
ApoI RAATTY 3 cut(s) 323, 405, 618
AspLEI GCGC 1 cut(s) 77
AsuHPI GGTGA 3 cut(s) 291, 500, 560
BaeGI GKGCMC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 3 cut(s) 64, 239, 724
BccI CCATC 1 cut(s) 249
BcoDI GTCTC 3 cut(s) 335, 463, 706
BfaI CTAG 1 cut(s) 290
BglII AGATCT 1 cut(s) 484
BisI GCNGC 3 cut(s) 78, 228, 713
BlsI GCNGC 3 cut(s) 79, 229, 714
BmiI GGNNCC 1 cut(s) 193
BmsI GCATC 2 cut(s) 20, 643
BpmI CTGGAG 1 cut(s) 371
BsaI GGTCTC 2 cut(s) 463, 706
BsaJI CCNNGG 1 cut(s) 187
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 521
Bse1I ACTGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 2 cut(s) 11, 655
BseLI CCNNNNNNNGG 2 cut(s) 247, 521
BseMII CTCAG 1 cut(s) 649
BseNI ACTGG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 3 cut(s) 64, 239, 724
BsgI GTGCAG 1 cut(s) 633
BshFI GGCC 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 716
BslI CCNNNNNNNGG 2 cut(s) 247, 521
BsmAI GTCTC 3 cut(s) 335, 463, 706
BsmI GAATGC 1 cut(s) 557
BsnI GGCC 1 cut(s) 279
Bso31I GGTCTC 2 cut(s) 463, 706
Bsp1286I GDGCHC 2 cut(s) 139, 315
Bsp143I GATC 3 cut(s) 484, 562, 688
Bsp19I CCATGG 1 cut(s) 187
BspACI CCGC 2 cut(s) 159, 511
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 1 cut(s) 648
BspLI GGNNCC 1 cut(s) 193
BspPI GGATC 2 cut(s) 557, 696
BspTNI GGTCTC 2 cut(s) 463, 706
BsrI ACTGG 1 cut(s) 285
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 3 cut(s) 484, 562, 688
BssT1I CCWWGG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 635
BstDSI CCRYGG 1 cut(s) 187
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 2 cut(s) 11, 655
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 3 cut(s) 487, 565, 691
BstMAI GTCTC 3 cut(s) 335, 463, 706
BstMBI GATC 3 cut(s) 484, 562, 688
BstMWI GCNNNNNNNGC 1 cut(s) 25
BstNSI RCATGY 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 64, 239, 724
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 2 cut(s) 188, 452
BstYI RGATCY 1 cut(s) 484
BsuRI GGCC 1 cut(s) 279
BtgI CCRYGG 1 cut(s) 187
BtsCI GGATG 2 cut(s) 11, 655
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 616
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 1 cut(s) 77
Csp6I GTAC 3 cut(s) 391, 464, 499
CviAII CATG 5 cut(s) 188, 266, 384, 545, 697
CviJI RGCY 8 cut(s) 19, 28, 279, 313, 355, 534, 634, 715
CviKI_1 RGCY 8 cut(s) 19, 28, 279, 313, 355, 534, 634, 715
CviQI GTAC 3 cut(s) 391, 464, 499
DdeI CTNAG 1 cut(s) 635
DpnI GATC 3 cut(s) 486, 564, 690
DpnII GATC 3 cut(s) 484, 562, 688
DraIII CACNNNGTG 1 cut(s) 548
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 2 cut(s) 463, 706
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoRI GAATTC 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 5 cut(s) 191, 269, 387, 548, 700
FatI CATG 5 cut(s) 187, 265, 383, 544, 696
FauI CCCGC 1 cut(s) 166
FauNDI CATATG 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 78, 228, 713
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 3 cut(s) 78, 228, 713
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 76
GluI GCNGC 3 cut(s) 78, 228, 713
GsuI CTGGAG 1 cut(s) 371
HaeIII GGCC 1 cut(s) 279
HapII CCGG 1 cut(s) 716
HhaI GCGC 1 cut(s) 77
Hin1II CATG 5 cut(s) 191, 269, 387, 548, 700
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HinfI GANTC 2 cut(s) 335, 596
HpaII CCGG 1 cut(s) 716
HphI GGTGA 3 cut(s) 291, 500, 560
Hpy166II GTNNAC 3 cut(s) 44, 299, 501
Hpy188I TCNGA 4 cut(s) 16, 340, 581, 638
Hpy188III TCNNGA 4 cut(s) 290, 350, 410, 738
Hpy8I GTNNAC 3 cut(s) 44, 299, 501
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4IV ACGT 1 cut(s) 363
HpyF10VI GCNNNNNNNGC 1 cut(s) 25
HpyF3I CTNAG 1 cut(s) 635
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 5 cut(s) 191, 269, 387, 548, 700
HspAI GCGC 1 cut(s) 75
Kzo9I GATC 3 cut(s) 484, 562, 688
LmnI GCTCC 2 cut(s) 85, 352
Lsp1109I GCAGC 3 cut(s) 64, 239, 724
LweI GCATC 2 cut(s) 20, 643
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 506
MalI GATC 3 cut(s) 486, 564, 690
MboI GATC 3 cut(s) 484, 562, 688
MboII GAAGA 6 cut(s) 87, 210, 236, 579, 721, 753
MfeI CAATTG 1 cut(s) 167
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 2 cut(s) 139, 315
MluCI AATT 6 cut(s) 5, 167, 323, 345, 405, 618
MnlI CCTC 1 cut(s) 640
Mph1103I ATGCAT 1 cut(s) 658
MseI TTAA 2 cut(s) 437, 659
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 716
MunI CAATTG 1 cut(s) 167
Mva1269I GAATGC 1 cut(s) 557
MwoI GCNNNNNNNGC 1 cut(s) 25
NcoI CCATGG 1 cut(s) 187
NdeI CATATG 1 cut(s) 100
NdeII GATC 3 cut(s) 484, 562, 688
NlaIII CATG 5 cut(s) 191, 269, 387, 548, 700
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 506
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 1 cut(s) 387
PciI ACATGT 1 cut(s) 383
PctI GAATGC 1 cut(s) 557
PfeI GAWTC 2 cut(s) 335, 596
PkrI GCNGC 3 cut(s) 79, 229, 714
PscI ACATGT 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 315
PspN4I GGNNCC 1 cut(s) 193
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 3 cut(s) 392, 465, 500
RsaNI GTAC 3 cut(s) 391, 464, 499
RseI CAYNNNNRTG 1 cut(s) 186
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 2 cut(s) 437, 659
SatI GCNGC 3 cut(s) 78, 228, 713
Sau3AI GATC 3 cut(s) 484, 562, 688
SduI GDGCHC 2 cut(s) 139, 315
SetI ASST 7 cut(s) 21, 30, 304, 315, 357, 366, 475
SfaNI GCATC 2 cut(s) 20, 643
SmiMI CAYNNNNRTG 1 cut(s) 186
Sse9I AATT 6 cut(s) 5, 167, 323, 345, 405, 618
SsiI CCGC 2 cut(s) 159, 511
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 315
StyI CCWWGG 1 cut(s) 187
TaaI ACNGT 1 cut(s) 611
TaiI ACGT 1 cut(s) 366
TaqI TCGA 3 cut(s) 175, 403, 739
TasI AATT 6 cut(s) 5, 167, 323, 345, 405, 618
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 2 cut(s) 335, 596
Tru1I TTAA 2 cut(s) 437, 659
Tru9I TTAA 2 cut(s) 437, 659
TscAI CASTG 2 cut(s) 77, 616
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 3 cut(s) 77, 227, 712
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 3 cut(s) 87, 609, 721
TspGWI ACGGA 2 cut(s) 285, 481
TspRI CASTG 2 cut(s) 77, 616
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 3 cut(s) 323, 405, 618
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 1 cut(s) 387
XcmI CCANNNNNNNNNTGG 1 cut(s) 542
XspI CTAG 1 cut(s) 290
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.