pycom09g06360

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008533
Physical Location & Seq
Reverse (-)
4676244 .. 4679328
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
ATGAGTATCCATCTGTCTCTCCGCCACCGCTCTTTCGCATACCTCAATCGCCGCCTCTCCTCCTCGCCCTTCTCCACCAGCACCATCTCCAAAATCCCCACCCTTTACGAACCCACCGCCGCCCCTACCACCACCCAAAACGATGCCGTCCCCACCACAACCCTCCAACTCCTCTCATGGGGCAGAGGTGCCTCCGGTCAGCTCGGCGGCGGCATCGAAGAAATCCGGCTCTACCCAACTCCAGTCGCCAACCTCGTCGTTCCCACCTCATCCTTCACTCTTTCACCAACCCAGGGCCGAATTCAAGCACCCAATTGCTCGAATCAAAAGAACAATGCCGACAGCGGTGTTGTGGAGGTGGGTATTTCTTGTGGGTTGTTCCACTCATCGCTGTTGGTGGATGGTAAGCTTTGGATTTGGGGTAAAGGCGACGGCGGTCGACTTGGTTTTGGGCATGAGAATTCTCTGTTTCTCCCGACTTTGAACCCCAATTTGGATTCGATTCGCTTCGTGGCGCTTGGCGGGTTGCATTCGGTGGCGCTCACCGCCACCGGGGAGGTCTATACTTGGGGTTATGGTGGTTTTGGTGCACTGGGGCATTCTGTTTATACTAGAGAGTTGTTCCCTAGATTGGTAAAAGGATCGTGGAGTGGGAAAATACGTCATATTTCGACTAGTGGGACGCATACTGGTGCCATCACGGAATCAGGAGAACTTTATACTTGGGGTCGAGATGAAGGGGATGGTAGATTGGGACTTGGTCCTAATCGAGGCCCAAACGAGGGTGGTGGACTCAGCATACCCTCCAAAGTAAAAGCATTACCTATGCCTGTGACTGCTGTTTCTTGCGGAGGTTTTTTCACAATGGCACTGACAGAAGATGGGGAACTTTGGAATTGGGGAGCAAACTCTAACTATGAGCTTGGAAGGGGCGATAAGGTTGGTGGTTGGACACCAAAACCAGTTCCTAGTCTTGCAGGCATTCGTATTATTCAGATAGAAAGTGGTGGATATCATTCTCTTGCGTTAACCGATGAAGGTAAAGTACTTTCTTGGGGCCATGGTGGTCATGGTCAGTTGGGCAACTCTTCTTTACAGAGTCAGAAAACACCTACAGTTGTTGAGGCTTTAACTGATGAGCGTGTTATCTATATTGCTTGCGGGGGTTCATCTTCAGCAGCTATAACAGATAAAGGAAAGTTGTTTATGTGGGGATACACCAAAGACTCTCAGCTAGGAGTACCTGGGCTGCCGGAGATCCAACCATCTCCTGTTGAAGTCAAGTTTTTAATGGAGGATGATGGACTGGGAGCCCACAATGTGCTGTCTGTTGCAGTTGGTGCTTCCCATGCCATGTGCTTGGTTTCAAGGGATGAATTGTCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1

Protein Analysis

463

Amino Acids

48.83

Weight (kDa)

6.5

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 56 - 453 6.4e-80 RCC1-like domain
RCC1 PF00415 134 - 181 1.7e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 168 - 196 4.2e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 185 - 233 7.3e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 236 - 291 1.4e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 278 - 305 5.6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 294 - 343 2.1e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 330 - 359 7.6e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 346 - 395 3.7e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 398 - 454 1.2e-06 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 188, 692
AccBSI CCGCTC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 439
AclWI GGATC 2 cut(s) 649, 1252
AcsI RAATTY 2 cut(s) 300, 460
AcuI CTGAAG 1 cut(s) 1158
AdeI CACNNNGTG 1 cut(s) 1321
AfaI GTAC 2 cut(s) 1047, 1242
AfiI CCNNNNNNNGG 8 cut(s) 178, 293, 493, 552, 631, 770, 781, 782
AgsI TTSAA 4 cut(s) 305, 484, 1277, 1368
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 2 cut(s) 291, 1243
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 5 cut(s) 202, 409, 922, 1181, 1234
AluI AGCT 5 cut(s) 202, 409, 922, 1181, 1234
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 1 cut(s) 21
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 2 cut(s) 649, 1252
AoxI GGCC 3 cut(s) 295, 772, 1057
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 2 cut(s) 1178, 1249
ApoI RAATTY 2 cut(s) 300, 460
AspLEI GCGC 2 cut(s) 517, 541
AspS9I GGNCC 4 cut(s) 295, 761, 773, 1057
AsuC2I CCSGG 1 cut(s) 553
AsuHPI GGTGA 2 cut(s) 276, 535
AvaII GGWCC 1 cut(s) 761
BaeGI GKGCMC 1 cut(s) 592
BanI GGYRCC 2 cut(s) 188, 692
BanII GRGCYC 1 cut(s) 1315
BarI GAAGNNNNNNTAC 2 cut(s) 1029, 1061
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 2 cut(s) 1190, 1236
BccI CCATC 8 cut(s) 18, 92, 395, 704, 737, 875, 1273, 1295
BceAI ACGGC 2 cut(s) 131, 448
BciT130I CCWGG 2 cut(s) 293, 1245
BciVI GTATCC 2 cut(s) 17, 1208
BcnI CCSGG 1 cut(s) 553
BcoDI GTCTC 1 cut(s) 21
BcuI ACTAGT 1 cut(s) 674
BfaI CTAG 5 cut(s) 612, 627, 675, 969, 1235
BfmI CTRYAG 1 cut(s) 1113
BfoI RGCGCY 2 cut(s) 518, 542
BfuI GTATCC 2 cut(s) 17, 1208
BisI GCNGC 6 cut(s) 52, 120, 208, 211, 1179, 1250
BlsI GCNGC 6 cut(s) 53, 121, 209, 212, 1180, 1251
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 3 cut(s) 293, 553, 1245
Bme18I GGWCC 1 cut(s) 761
BmgT120I GGNCC 4 cut(s) 295, 761, 773, 1057
BmiI GGNNCC 4 cut(s) 190, 694, 1058, 1312
BmrFI CCNGG 3 cut(s) 293, 553, 1245
BmrI ACTGGG 2 cut(s) 602, 1316
BmsI GCATC 2 cut(s) 133, 222
BmuI ACTGGG 2 cut(s) 602, 1316
BoxI GACNNNNGTC 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 553
BsaJI CCNNGG 5 cut(s) 291, 292, 552, 1060, 1244
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 293, 493, 552, 631, 770, 781, 782
Bse1I ACTGG 5 cut(s) 242, 597, 694, 962, 1311
BseBI CCWGG 2 cut(s) 293, 1245
BseDI CCNNGG 5 cut(s) 291, 292, 552, 1060, 1244
BseGI GGATG 5 cut(s) 269, 406, 748, 1303, 1378
BseLI CCNNNNNNNGG 8 cut(s) 178, 293, 493, 552, 631, 770, 781, 782
BseMII CTCAG 2 cut(s) 808, 1244
BseNI ACTGG 5 cut(s) 242, 597, 694, 962, 1311
BseRI GAGGAG 3 cut(s) 49, 52, 161
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 2 cut(s) 1190, 1236
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 297, 774, 1059
BshNI GGYRCC 2 cut(s) 188, 692
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 592
BsiSI CCGG 4 cut(s) 195, 226, 552, 1253
BslFI GGGAC 3 cut(s) 134, 694, 768
BslI CCNNNNNNNGG 8 cut(s) 178, 293, 493, 552, 631, 770, 781, 782
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 3 cut(s) 134, 694, 768
BsmI GAATGC 3 cut(s) 529, 598, 981
BsnI GGCC 3 cut(s) 297, 774, 1059
Bsp1286I GDGCHC 2 cut(s) 592, 1315
Bsp143I GATC 2 cut(s) 641, 1257
Bsp19I CCATGG 1 cut(s) 1060
BspANI GGCC 3 cut(s) 297, 774, 1059
BspCNI CTCAG 2 cut(s) 807, 1243
BspLI GGNNCC 4 cut(s) 190, 694, 1058, 1312
BspPI GGATC 2 cut(s) 649, 1252
BspT107I GGYRCC 2 cut(s) 188, 692
BsrBI CCGCTC 1 cut(s) 30
BsrI ACTGG 5 cut(s) 242, 597, 694, 962, 1311
BssECI CCNNGG 5 cut(s) 291, 292, 552, 1060, 1244
BssMI GATC 2 cut(s) 641, 1257
BssT1I CCWWGG 1 cut(s) 1060
Bst2UI CCWGG 2 cut(s) 293, 1245
Bst4CI ACNGT 1 cut(s) 1117
Bst6I CTCTTC 1 cut(s) 1093
BstAPI GCANNNNNTGC 1 cut(s) 1340
BstC8I GCNNGC 2 cut(s) 979, 1159
BstDEI CTNAG 2 cut(s) 794, 1230
BstDSI CCRYGG 1 cut(s) 1060
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 5 cut(s) 269, 406, 748, 1303, 1378
BstH2I RGCGCY 2 cut(s) 518, 542
BstHHI GCGC 2 cut(s) 517, 541
BstKTI GATC 2 cut(s) 644, 1260
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 641, 1257
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 3 cut(s) 545, 1340, 1349
BstNI CCWGG 2 cut(s) 293, 1245
BstPAI GACNNNNGTC 1 cut(s) 435
BstSCI CCNGG 3 cut(s) 291, 551, 1243
BstSFI CTRYAG 1 cut(s) 1113
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 2 cut(s) 1190, 1236
BstX2I RGATCY 1 cut(s) 1257
BstXI CCANNNNNNTGG 1 cut(s) 1360
BstYI RGATCY 1 cut(s) 1257
BsuI GTATCC 2 cut(s) 17, 1208
BsuRI GGCC 3 cut(s) 297, 774, 1059
BtgI CCRYGG 1 cut(s) 1060
BtgZI GCGATG 1 cut(s) 372
BtsCI GGATG 5 cut(s) 269, 406, 748, 1303, 1378
BtsIMutI CAGTG 2 cut(s) 590, 869
Cac8I GCNNGC 2 cut(s) 979, 1159
CfoI GCGC 2 cut(s) 517, 541
Cfr13I GGNCC 4 cut(s) 295, 761, 773, 1057
CseI GACGC 1 cut(s) 691
Csp6I GTAC 2 cut(s) 1046, 1241
CviAII CATG 6 cut(s) 177, 455, 1061, 1070, 1349, 1354
CviQI GTAC 2 cut(s) 1046, 1241
DdeI CTNAG 2 cut(s) 794, 1230
DpnI GATC 2 cut(s) 643, 1259
DpnII GATC 2 cut(s) 641, 1257
DraIII CACNNNGTG 1 cut(s) 1321
Eam1104I CTCTTC 1 cut(s) 1093
EarI CTCTTC 1 cut(s) 1093
EciI GGCGGA 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 1060
Eco24I GRGCYC 1 cut(s) 1315
Eco32I GATATC 1 cut(s) 1013
Eco47I GGWCC 1 cut(s) 761
Eco57I CTGAAG 1 cut(s) 1158
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRI GAATTC 2 cut(s) 300, 460
EcoRII CCWGG 2 cut(s) 291, 1243
EcoRV GATATC 1 cut(s) 1013
EcoT14I CCWWGG 1 cut(s) 1060
EcoT38I GRGCYC 1 cut(s) 1315
ErhI CCWWGG 1 cut(s) 1060
FaeI CATG 6 cut(s) 180, 458, 1064, 1073, 1352, 1357
FaqI GGGAC 3 cut(s) 134, 694, 768
FatI CATG 6 cut(s) 176, 454, 1060, 1069, 1348, 1353
FauI CCCGC 2 cut(s) 515, 1154
FblI GTMKAC 1 cut(s) 439
Fnu4HI GCNGC 6 cut(s) 52, 120, 208, 211, 1179, 1250
FokI GGATG 5 cut(s) 256, 413, 755, 1310, 1385
FriOI GRGCYC 1 cut(s) 1315
Fsp4HI GCNGC 6 cut(s) 52, 120, 208, 211, 1179, 1250
FspBI CTAG 5 cut(s) 612, 627, 675, 969, 1235
GlaI GCGC 2 cut(s) 516, 540
GluI GCNGC 6 cut(s) 52, 120, 208, 211, 1179, 1250
GsuI CTGGAG 1 cut(s) 225
HaeII RGCGCY 2 cut(s) 518, 542
HaeIII GGCC 3 cut(s) 297, 774, 1059
HapII CCGG 4 cut(s) 195, 226, 552, 1253
HgaI GACGC 1 cut(s) 691
HhaI GCGC 2 cut(s) 517, 541
Hin1II CATG 6 cut(s) 180, 458, 1064, 1073, 1352, 1357
Hin6I GCGC 2 cut(s) 515, 539
HinP1I GCGC 2 cut(s) 515, 539
HincII GTYRAC 2 cut(s) 440, 1029
HindII GTYRAC 2 cut(s) 440, 1029
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 7 cut(s) 322, 497, 502, 704, 792, 1099, 1226
HpaI GTTAAC 1 cut(s) 1029
HpaII CCGG 4 cut(s) 195, 226, 552, 1253
HphI GGTGA 2 cut(s) 276, 535
Hpy166II GTNNAC 4 cut(s) 440, 590, 791, 1029
Hpy188I TCNGA 2 cut(s) 996, 1104
Hpy188III TCNNGA 3 cut(s) 475, 708, 731
Hpy8I GTNNAC 4 cut(s) 440, 590, 791, 1029
Hpy99I CGWCG 2 cut(s) 260, 434
HpyAV CCTTC 5 cut(s) 79, 283, 731, 921, 1031
HpyCH4III ACNGT 1 cut(s) 1117
HpyCH4IV ACGT 1 cut(s) 661
HpyCH4V TGCA 4 cut(s) 529, 590, 977, 1334
HpyF10VI GCNNNNNNNGC 3 cut(s) 545, 1340, 1349
HpyF3I CTNAG 2 cut(s) 794, 1230
HpySE526I ACGT 1 cut(s) 661
Hsp92II CATG 6 cut(s) 180, 458, 1064, 1073, 1352, 1357
HspAI GCGC 2 cut(s) 515, 539
KspAI GTTAAC 1 cut(s) 1029
Kzo9I GATC 2 cut(s) 641, 1257
LmnI GCTCC 2 cut(s) 902, 1310
Lsp1109I GCAGC 2 cut(s) 1190, 1236
LweI GCATC 2 cut(s) 133, 222
MaeI CTAG 5 cut(s) 612, 627, 675, 969, 1235
MaeII ACGT 1 cut(s) 661
MaeIII GTNAC 1 cut(s) 832
MalI GATC 2 cut(s) 643, 1259
MbiI CCGCTC 1 cut(s) 30
MboI GATC 2 cut(s) 641, 1257
MboII GAAGA 4 cut(s) 230, 890, 1080, 1164
MfeI CAATTG 1 cut(s) 313
MflI RGATCY 1 cut(s) 1257
MhlI GDGCHC 2 cut(s) 592, 1315
MluCI AATT 6 cut(s) 300, 313, 460, 490, 895, 1376
MlyI GAGTC 3 cut(s) 786, 1108, 1220
MmeI TCCRAC 3 cut(s) 190, 929, 1285
MseI TTAA 3 cut(s) 1028, 1130, 1289
MslI CAYNNNNRTG 1 cut(s) 690
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 4 cut(s) 195, 226, 552, 1253
MspR9I CCNGG 3 cut(s) 293, 553, 1245
MunI CAATTG 1 cut(s) 313
Mva1269I GAATGC 3 cut(s) 529, 598, 981
MvaI CCWGG 2 cut(s) 293, 1245
MwoI GCNNNNNNNGC 3 cut(s) 545, 1340, 1349
NciI CCSGG 1 cut(s) 553
NcoI CCATGG 1 cut(s) 1060
NdeII GATC 2 cut(s) 641, 1257
NlaIII CATG 6 cut(s) 180, 458, 1064, 1073, 1352, 1357
NlaIV GGNNCC 4 cut(s) 190, 694, 1058, 1312
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 832
PasI CCCWGGG 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 3 cut(s) 529, 598, 981
PfeI GAWTC 4 cut(s) 322, 497, 502, 704
PflFI GACNNNGTC 1 cut(s) 759
PkrI GCNGC 6 cut(s) 53, 121, 209, 212, 1180, 1251
PleI GAGTC 3 cut(s) 786, 1107, 1220
PpsI GAGTC 3 cut(s) 786, 1107, 1220
PshAI GACNNNNGTC 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 291, 1243
PspGI CCWGG 2 cut(s) 291, 1243
PspN4I GGNNCC 4 cut(s) 190, 694, 1058, 1312
PspPI GGNCC 4 cut(s) 295, 761, 773, 1057
PsuI RGATCY 1 cut(s) 1257
PsyI GACNNNGTC 1 cut(s) 759
RsaI GTAC 2 cut(s) 1047, 1242
RsaNI GTAC 2 cut(s) 1046, 1241
RseI CAYNNNNRTG 1 cut(s) 690
SalI GTCGAC 1 cut(s) 438
SaqAI TTAA 3 cut(s) 1028, 1130, 1289
SatI GCNGC 6 cut(s) 52, 120, 208, 211, 1179, 1250
Sau3AI GATC 2 cut(s) 641, 1257
Sau96I GGNCC 4 cut(s) 295, 761, 773, 1057
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 3 cut(s) 786, 1108, 1220
ScrFI CCNGG 3 cut(s) 293, 553, 1245
SduI GDGCHC 2 cut(s) 592, 1315
SfaNI GCATC 2 cut(s) 133, 222
SfcI CTRYAG 1 cut(s) 1113
SinI GGWCC 1 cut(s) 761
SmiMI CAYNNNNRTG 1 cut(s) 690
SpeI ACTAGT 1 cut(s) 674
Sse9I AATT 6 cut(s) 300, 313, 460, 490, 895, 1376
SspMI CTAG 5 cut(s) 612, 627, 675, 969, 1235
StyD4I CCNGG 3 cut(s) 291, 551, 1243
StyI CCWWGG 1 cut(s) 1060
TaaI ACNGT 1 cut(s) 1117
TaiI ACGT 1 cut(s) 664
TaqI TCGA 7 cut(s) 216, 320, 439, 500, 671, 730, 769
TasI AATT 6 cut(s) 300, 313, 460, 490, 895, 1376
TatI WGTACW 1 cut(s) 1045
TauI GCSGC 4 cut(s) 54, 122, 210, 213
TfiI GAWTC 4 cut(s) 322, 497, 502, 704
Tru1I TTAA 3 cut(s) 1028, 1130, 1289
Tru9I TTAA 3 cut(s) 1028, 1130, 1289
TscAI CASTG 2 cut(s) 597, 876
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 2 cut(s) 1178, 1249
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 4 cut(s) 750, 1050, 1158, 1389
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 2 cut(s) 597, 876
Tth111I GACNNNGTC 1 cut(s) 759
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 761
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 2 cut(s) 300, 460
XcmI CCANNNNNNNNNTGG 1 cut(s) 1067
XmiI GTMKAC 1 cut(s) 439
XspI CTAG 5 cut(s) 612, 627, 675, 969, 1235
ZrmI AGTACT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.