pycom09g06600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007987
Physical Location & Seq
Reverse (-)
4833219 .. 4837674
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1761 bp
ATGCCTCAAACCCTTTTCTTAAGGCCCTTTATTTCATCAGCTCCTTTGGATAATTGGAGTAAATTGAAAGAATGCACAAGTTCTATAGGTTCCAATGTCAAGGTGCGTGGAAGAGTGGGGATAGTTTGTATGGGCATGCTGGCACCAAGAAAGTTCTTGCAGAAAAGGAAGAAGGTAGAAGTTTTCAAAGATGCTGCTGATGAAGCCGAGCAGAAGAACTGGAGGAGACTTATGAATGAAATTGAGGAGACAGGTTCTGCAGTTTCTGTGCTCAAAAGTGAAAAGCTCAAGGACAAGACTATTCCCAAGGATGTTGTCCTTGGAACTTTGGTTAGATTTAAGCAACTGAAAAAATGGAACCTAGTCAGCCAGATTCTCGAATGGCTCCAAACTCAGAACTGGTGGGACTTCAGCAAAATGGATTTCCTGATGCTTATAACAGCTTATGGAAAGCAAGGGCACTACACGAGGGCTGAGAAGCTTTTAAGTTTGATGAATGAGAAAGGTCATCCACCAAGTGTAATATCTCATACTGCTCTTATGGAAGCATATGGAATAGGAGGCCGATATAACAATGCTGAAGCAATATTTAGAAGGATGCAGTCTTCTGGCCCTGAACCATCTGCTGTGACATATCAAATAATACTAAAGATATTTGTTGAGGGATGTAAGTTCAAGGAAGCTGAAGAAATATTTGAGACTCTTCTAAACAAGGAAAAATCACCTTTAAATCCAGACCAAAAGATGTTCCACATGATGATTTATATGTATAAGAAGGCTGGGAGCTATGATAAAGCTCGGAAGATGTTCGCAATGATGTCTGAGAGAGGGGTTCCACAATCGACGGTTACTTATAATAGCTTGATGTCATTTGAAAATAATTACAAGGAAGTCTCCAAGATGTATAACCAGATGCAAAGAGCTGGTCTCCGACCTGACGTAGTGAGTTATGCCTTACTCATTAGTGCTTATGGGAAAGCTAGAAGAGAGGAAGAAGCCTTAGCTGTTTTTGAGGAGATGCTTGATGCTGGTGTCAGACCAACCCGCAAAGCTTATAACATTTTGCTTGACGCATTTGCAGTATCAGGAATGGTGGACCAAGCTCGAACAGTTTTTAAAAGCATGAGAAGGGACAGGTTTAATCCTGATCTGTGCTCTTATACAACTATGCTGTCGGCATATGTAAATGCATCTGACATGGAGGGTGCCGAGAAATTCTTCATAAGAATAAAACAAGATGCATTTAAACCCAATGTCGTCACTTATGGGACATTAATCAAAGGGTATGCTAAGACAAGTAATATCGAGAAGATGATGGAGAAATATGAGGAAATGCAGGCATCTGGTATCAAACCAAATCAAACAATCTTGACAACAATCATGGATGCATATGGAAAAAACAGAGATTTTGGCAGTGCTGTTGTTTGGTACCAGGAAATGGAATCCTGTAGGCTTCCGCCTGATCAGAAAGCAAAGAATATCCTTTTGTCTTTGGCGAAAACAGCAGAGGAACAGAAGGTGGCTAACCAGCTTGTAGGAAATTTGGATCAGAGTAGCAATGAACAAGGATCTAGTAAGTATCTGGTGCCTACCGATGAGAATGATAGTGAGGATGAGGATGAGGATGAAAAGTATGATGATGAATTAGATGGTCCTTCACTGGTCACTTCATCAAATGCGGAGCAAAAACATGAACTGGTTTATCTCAACGGTGTTAGCGAGAAAAACCTTGAGGGCTTACTTGCAGCTGCAGATTTGTAA

Protein Analysis

587

Amino Acids

66.66

Weight (kDa)

7.52

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 144 - 184 5.3e-06 PPR repeat family
PPR_2 PF13041 172 - 218 5.4e-07 PPR repeat family
PPR_3 PF13812 172 - 220 6.5e-06 Pentatricopeptide repeat domain
PPR_long PF17177 244 - 365 4.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 245 - 289 3.7e-06 PPR repeat family
PPR_3 PF13812 303 - 359 6.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 309 - 340 2.4e-06 PPR repeat
PPR_2 PF13041 312 - 360 8.6e-12 PPR repeat family
PPR PF01535 315 - 345 2.2e-06 PPR repeat
PPR_2 PF13041 351 - 395 6.4e-09 PPR repeat family
TPR_24 PF23276 360 - 480 6.8e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 365 - 463 1.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 371 - 429 1.4e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 382 - 431 3.1e-12 PPR repeat family
PPR_3 PF13812 411 - 467 8.8e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 417 - 454 1.1e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 437, 855, 1056
Acc65I GGTACC 1 cut(s) 1428
AccB1I GGYRCC 4 cut(s) 142, 1205, 1428, 1585
AccB7I CCANNNNNTGG 1 cut(s) 1438
AciI CCGC 3 cut(s) 1045, 1457, 1679
AclWI GGATC 2 cut(s) 1554, 1576
AcsI RAATTY 2 cut(s) 1214, 1540
AcuI CTGAAG 3 cut(s) 394, 600, 705
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 1 cut(s) 1430
AfiI CCNNNNNNNGG 1 cut(s) 1438
AflII CTTAAG 1 cut(s) 19
AgsI TTSAA 4 cut(s) 67, 187, 676, 875
AjnI CCWGG 1 cut(s) 1431
Alw21I GWGCWC 2 cut(s) 273, 1157
Alw26I GTCTC 5 cut(s) 220, 242, 692, 898, 932
AlwI GGATC 2 cut(s) 1554, 1576
AlwNI CAGNNNCTG 2 cut(s) 257, 266
AoxI GGCC 3 cut(s) 23, 562, 610
ApeKI GCWGC 3 cut(s) 194, 1745, 1748
ApoI RAATTY 2 cut(s) 1214, 1540
ArsI GACNNNNNNTTYG 2 cut(s) 910, 942
AseI ATTAAT 1 cut(s) 1274
Asp700I GAANNNNTTC 2 cut(s) 806, 1217
Asp718I GGTACC 1 cut(s) 1428
AspS9I GGNCC 4 cut(s) 24, 611, 1096, 1652
AsuHPI GGTGA 1 cut(s) 714
AvaII GGWCC 2 cut(s) 1096, 1652
BaeGI GKGCMC 1 cut(s) 462
BanI GGYRCC 4 cut(s) 142, 1205, 1428, 1585
BauI CACGAG 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 2 cut(s) 273, 1157
BbvI GCAGC 3 cut(s) 181, 1735, 1757
BccI CCATC 3 cut(s) 628, 1309, 1643
BciT130I CCWGG 1 cut(s) 1433
BclI TGATCA 1 cut(s) 1462
BcoDI GTCTC 5 cut(s) 220, 242, 692, 898, 932
BfaI CTAG 3 cut(s) 362, 981, 1572
BfmI CTRYAG 4 cut(s) 84, 258, 1447, 1749
BfrI CTTAAG 1 cut(s) 19
BisI GCNGC 3 cut(s) 195, 1746, 1749
BlsI GCNGC 3 cut(s) 196, 1747, 1750
Bme1390I CCNGG 1 cut(s) 1433
Bme18I GGWCC 2 cut(s) 1096, 1652
BmgT120I GGNCC 4 cut(s) 24, 611, 1096, 1652
BmiI GGNNCC 8 cut(s) 91, 144, 359, 386, 834, 1207, 1430, 1587
BmrFI CCNGG 1 cut(s) 1433
BpiI GAAGAC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 912, 944
BpmI CTGGAG 1 cut(s) 241
Bpu10I CCTNAGC 1 cut(s) 1000
BpuEI CTTGAG 2 cut(s) 272, 1751
BsaI GGTCTC 1 cut(s) 932
BsaJI CCNNGG 2 cut(s) 306, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 1438
Bse1I ACTGG 4 cut(s) 224, 404, 1665, 1701
Bse3DI GCAATG 2 cut(s) 819, 1564
BseBI CCWGG 1 cut(s) 1433
BseDI CCNNGG 2 cut(s) 306, 319
BseGI GGATG 8 cut(s) 316, 508, 603, 671, 1390, 1618, 1624, 1630
BseLI CCNNNNNNNGG 1 cut(s) 1438
BseMI GCAATG 2 cut(s) 819, 1564
BseMII CTCAG 3 cut(s) 407, 465, 813
BseNI ACTGG 4 cut(s) 224, 404, 1665, 1701
BseRI GAGGAG 3 cut(s) 238, 260, 1028
BseSI GKGCMC 1 cut(s) 462
BseXI GCAGC 3 cut(s) 181, 1735, 1757
BseYI CCCAGC 1 cut(s) 779
BshFI GGCC 3 cut(s) 25, 564, 612
BshNI GGYRCC 4 cut(s) 142, 1205, 1428, 1585
BsiHKAI GWGCWC 2 cut(s) 273, 1157
BslFI GGGAC 3 cut(s) 419, 1145, 1282
BslI CCNNNNNNNGG 1 cut(s) 1438
BsmAI GTCTC 5 cut(s) 220, 242, 692, 898, 932
BsmFI GGGAC 3 cut(s) 419, 1145, 1282
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 3 cut(s) 25, 564, 612
Bso31I GGTCTC 1 cut(s) 932
Bsp1286I GDGCHC 3 cut(s) 273, 462, 1157
Bsp143I GATC 4 cut(s) 1147, 1462, 1546, 1568
BspACI CCGC 3 cut(s) 1045, 1457, 1679
BspANI GGCC 3 cut(s) 25, 564, 612
BspCNI CTCAG 3 cut(s) 406, 466, 814
BspLI GGNNCC 8 cut(s) 91, 144, 359, 386, 834, 1207, 1430, 1587
BspMAI CTGCAG 2 cut(s) 262, 1753
BspPI GGATC 2 cut(s) 1554, 1576
BspT107I GGYRCC 4 cut(s) 142, 1205, 1428, 1585
BspTI CTTAAG 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 932
BsrDI GCAATG 2 cut(s) 819, 1564
BsrI ACTGG 4 cut(s) 224, 404, 1665, 1701
BssECI CCNNGG 2 cut(s) 306, 319
BssMI GATC 4 cut(s) 1147, 1462, 1546, 1568
BssSI CACGAG 1 cut(s) 466
BssT1I CCWWGG 2 cut(s) 306, 319
Bst2BI CACGAG 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 1433
Bst4CI ACNGT 3 cut(s) 847, 1111, 1712
Bst6I CTCTTC 3 cut(s) 106, 708, 979
BstAFI CTTAAG 1 cut(s) 19
BstC8I GCNNGC 3 cut(s) 137, 141, 1338
BstDEI CTNAG 5 cut(s) 393, 474, 822, 1000, 1290
BstF5I GGATG 8 cut(s) 316, 508, 603, 671, 1390, 1618, 1624, 1630
BstKTI GATC 4 cut(s) 1150, 1465, 1549, 1571
BstMAI GTCTC 5 cut(s) 220, 242, 692, 898, 932
BstMBI GATC 4 cut(s) 1147, 1462, 1546, 1568
BstMWI GCNNNNNNNGC 2 cut(s) 203, 1502
BstNI CCWGG 1 cut(s) 1433
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 1431
BstSFI CTRYAG 4 cut(s) 84, 258, 1447, 1749
BstSLI GKGCMC 1 cut(s) 462
BstV1I GCAGC 3 cut(s) 181, 1735, 1757
BstV2I GAAGAC 1 cut(s) 597
BstX2I RGATCY 1 cut(s) 1568
BstYI RGATCY 1 cut(s) 1568
BsuRI GGCC 3 cut(s) 25, 564, 612
BtsCI GGATG 8 cut(s) 316, 508, 603, 671, 1390, 1618, 1624, 1630
BtsI GCAGTG 1 cut(s) 1420
BtsIMutI CAGTG 2 cut(s) 1420, 1658
Cac8I GCNNGC 3 cut(s) 137, 141, 1338
CaiI CAGNNNCTG 2 cut(s) 257, 266
Cfr13I GGNCC 4 cut(s) 24, 611, 1096, 1652
CseI GACGC 1 cut(s) 1079
Csp6I GTAC 1 cut(s) 1429
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviAII CATG 6 cut(s) 136, 754, 1123, 1198, 1381, 1691
CviQI GTAC 1 cut(s) 1429
DdeI CTNAG 5 cut(s) 393, 474, 822, 1000, 1290
DpnI GATC 4 cut(s) 1149, 1464, 1548, 1570
DpnII GATC 4 cut(s) 1147, 1462, 1546, 1568
DraI TTTAAA 3 cut(s) 729, 1117, 1246
DraIII CACNNNGTG 1 cut(s) 518
Eam1104I CTCTTC 3 cut(s) 106, 708, 979
EarI CTCTTC 3 cut(s) 106, 708, 979
EciI GGCGGA 1 cut(s) 1446
Eco130I CCWWGG 2 cut(s) 306, 319
Eco31I GGTCTC 1 cut(s) 932
Eco47I GGWCC 2 cut(s) 1096, 1652
Eco57I CTGAAG 3 cut(s) 394, 600, 705
EcoO109I RGGNCCY 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 1431
EcoT14I CCWWGG 2 cut(s) 306, 319
EcoT22I ATGCAT 3 cut(s) 1192, 1243, 1390
ErhI CCWWGG 2 cut(s) 306, 319
FaeI CATG 6 cut(s) 139, 757, 1126, 1201, 1384, 1694
FaqI GGGAC 3 cut(s) 419, 1145, 1282
FatI CATG 6 cut(s) 135, 753, 1122, 1197, 1380, 1690
FauI CCCGC 1 cut(s) 1052
FauNDI CATATG 3 cut(s) 550, 1180, 1390
FbaI TGATCA 1 cut(s) 1462
Fnu4HI GCNGC 3 cut(s) 195, 1746, 1749
FokI GGATG 8 cut(s) 323, 495, 610, 678, 1397, 1625, 1631, 1637
Fsp4HI GCNGC 3 cut(s) 195, 1746, 1749
FspBI CTAG 3 cut(s) 362, 981, 1572
GluI GCNGC 3 cut(s) 195, 1746, 1749
GsaI CCCAGC 1 cut(s) 783
GsuI CTGGAG 1 cut(s) 241
HaeIII GGCC 3 cut(s) 25, 564, 612
HgaI GACGC 1 cut(s) 1079
Hin1II CATG 6 cut(s) 139, 757, 1126, 1201, 1384, 1694
HindIII AAGCTT 2 cut(s) 479, 1050
HinfI GANTC 3 cut(s) 373, 700, 1442
HphI GGTGA 1 cut(s) 714
Hpy166II GTNNAC 1 cut(s) 1096
Hpy188I TCNGA 8 cut(s) 396, 801, 823, 932, 1037, 1195, 1467, 1551
Hpy188III TCNNGA 7 cut(s) 377, 427, 734, 1086, 1145, 1306, 1369
Hpy8I GTNNAC 1 cut(s) 1096
Hpy99I CGWCG 1 cut(s) 847
HpyAV CCTTC 6 cut(s) 166, 588, 769, 1122, 1510, 1665
HpyCH4III ACNGT 3 cut(s) 847, 1111, 1712
HpyCH4IV ACGT 1 cut(s) 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 1502
HpyF3I CTNAG 5 cut(s) 393, 474, 822, 1000, 1290
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 6 cut(s) 139, 757, 1126, 1201, 1384, 1694
KpnI GGTACC 1 cut(s) 1432
Ksp22I TGATCA 1 cut(s) 1462
Kzo9I GATC 4 cut(s) 1147, 1462, 1546, 1568
LmnI GCTCC 4 cut(s) 46, 390, 783, 1681
Lsp1109I GCAGC 3 cut(s) 181, 1735, 1757
MaeI CTAG 3 cut(s) 362, 981, 1572
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 4 cut(s) 628, 847, 1258, 1663
MalI GATC 4 cut(s) 1149, 1464, 1548, 1570
MboI GATC 4 cut(s) 1147, 1462, 1546, 1568
MflI RGATCY 1 cut(s) 1568
MhlI GDGCHC 3 cut(s) 273, 462, 1157
MluCI AATT 7 cut(s) 52, 62, 240, 880, 1214, 1540, 1643
MlyI GAGTC 1 cut(s) 694
MmeI TCCRAC 1 cut(s) 955
Mph1103I ATGCAT 3 cut(s) 1192, 1243, 1390
MroXI GAANNNNTTC 2 cut(s) 806, 1217
MseI TTAA 8 cut(s) 20, 339, 485, 728, 1116, 1140, 1245, 1274
MspA1I CMGCKG 1 cut(s) 1748
MspCI CTTAAG 1 cut(s) 19
MspR9I CCNGG 1 cut(s) 1433
Mva1269I GAATGC 1 cut(s) 77
MvaI CCWGG 1 cut(s) 1433
MwoI GCNNNNNNNGC 2 cut(s) 203, 1502
NdeI CATATG 3 cut(s) 550, 1180, 1390
NdeII GATC 4 cut(s) 1147, 1462, 1546, 1568
NlaIII CATG 6 cut(s) 139, 757, 1126, 1201, 1384, 1694
NlaIV GGNNCC 8 cut(s) 91, 144, 359, 386, 834, 1207, 1430, 1587
NmeAIII GCCGAG 2 cut(s) 232, 1234
NmuCI GTSAC 3 cut(s) 628, 1258, 1663
NsiI ATGCAT 3 cut(s) 1192, 1243, 1390
NspI RCATGY 1 cut(s) 139
PaeI GCATGC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 1716
PctI GAATGC 1 cut(s) 77
PdmI GAANNNNTTC 2 cut(s) 806, 1217
PfeI GAWTC 2 cut(s) 373, 1442
PflMI CCANNNNNTGG 1 cut(s) 1438
PkrI GCNGC 3 cut(s) 196, 1747, 1750
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
PshBI ATTAAT 1 cut(s) 1274
PsiI TTATAA 3 cut(s) 437, 855, 1056
Psp6I CCWGG 1 cut(s) 1431
PspFI CCCAGC 1 cut(s) 779
PspGI CCWGG 1 cut(s) 1431
PspN4I GGNNCC 8 cut(s) 91, 144, 359, 386, 834, 1207, 1430, 1587
PspPI GGNCC 4 cut(s) 24, 611, 1096, 1652
PstI CTGCAG 2 cut(s) 262, 1753
PstNI CAGNNNCTG 2 cut(s) 257, 266
PsuI RGATCY 1 cut(s) 1568
PvuII CAGCTG 1 cut(s) 1748
RsaI GTAC 1 cut(s) 1430
RsaNI GTAC 1 cut(s) 1429
SaqAI TTAA 8 cut(s) 20, 339, 485, 728, 1116, 1140, 1245, 1274
SatI GCNGC 3 cut(s) 195, 1746, 1749
Sau3AI GATC 4 cut(s) 1147, 1462, 1546, 1568
Sau96I GGNCC 4 cut(s) 24, 611, 1096, 1652
SchI GAGTC 1 cut(s) 694
ScrFI CCNGG 1 cut(s) 1433
SduI GDGCHC 3 cut(s) 273, 462, 1157
SfcI CTRYAG 4 cut(s) 84, 258, 1447, 1749
SinI GGWCC 2 cut(s) 1096, 1652
SmlI CTYRAG 3 cut(s) 19, 287, 1730
SmoI CTYRAG 3 cut(s) 19, 287, 1730
SphI GCATGC 1 cut(s) 139
Sse9I AATT 7 cut(s) 52, 62, 240, 880, 1214, 1540, 1643
SsiI CCGC 3 cut(s) 1045, 1457, 1679
SspI AATATT 2 cut(s) 588, 693
SspMI CTAG 3 cut(s) 362, 981, 1572
StyD4I CCNGG 1 cut(s) 1431
StyI CCWWGG 2 cut(s) 306, 319
TaaI ACNGT 3 cut(s) 847, 1111, 1712
TaiI ACGT 1 cut(s) 942
TaqI TCGA 4 cut(s) 378, 842, 1105, 1305
TasI AATT 7 cut(s) 52, 62, 240, 880, 1214, 1540, 1643
TfiI GAWTC 2 cut(s) 373, 1442
Tru1I TTAA 8 cut(s) 20, 339, 485, 728, 1116, 1140, 1245, 1274
Tru9I TTAA 8 cut(s) 20, 339, 485, 728, 1116, 1140, 1245, 1274
TscAI CASTG 2 cut(s) 1420, 1665
TseFI GTSAC 3 cut(s) 628, 1258, 1663
TseI GCWGC 3 cut(s) 194, 1745, 1748
Tsp45I GTSAC 3 cut(s) 628, 1258, 1663
TspRI CASTG 2 cut(s) 1420, 1665
Van91I CCANNNNNTGG 1 cut(s) 1438
Vha464I CTTAAG 1 cut(s) 19
VpaK11BI GGWCC 2 cut(s) 1096, 1652
VspI ATTAAT 1 cut(s) 1274
XapI RAATTY 2 cut(s) 1214, 1540
XceI RCATGY 1 cut(s) 139
XmnI GAANNNNTTC 2 cut(s) 806, 1217
XspI CTAG 3 cut(s) 362, 981, 1572
Zsp2I ATGCAT 3 cut(s) 1192, 1243, 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.