pycom09g11840

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0018397
Physical Location & Seq
Forward (+)
10020616 .. 10021857
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
ATGTCGACATTGATGAACATTAGTATTAATAATGTCCCTGAGTCGGTATTGGTTGAAATTCTTTGTCGACTTCCTTGCGCTAATTTCATTTTTCAATGCAAGTGCGTGTGCAAGCGTTGGTTCACTCTCATCTCCAGCCGTTATTTTGTTAACCGCTTTTTGCGTCTGCAAATTGATAACAAAATGCCGGTGGTATGCACTTTGATAAACCACAATGGGGAGGAATTCCTTGACAGAATTCAGCCCTCGTCGGAAGTGATACCTCATATGTTGAAAAGGCTCCAGAGTTTGCACGGTTTGAGTAAAGAGCCAGTTGTGGTAGCCACTTACAATGACTTGATTTTGTGCTCTAATGGCTTGTTTGATCAAACTGAATACTACATATGCAATCCATACACCACGCAATGGGTTGCCCTTCCTCGCCCCCGTTGGGGACGCGACACCATAGGCACCATGCCAGTGGGACTCATCTGCGACAGTCCTTACTATAAGGAGGACGATCAGAATGACATTGTGGAGCTTAATGATAACTATAGGTACAAGGTTGTGAGAATTCTTCACCCTAATGATGAATTTAACTTTGATCCTGATGGATATTTCTACAACTTCAAGGTGGAGATTTTCTCTTCAGAGACAGGCAAATGGAGAGAGTTAGTTGTATCATCCCCACAAGGAATTATATATGGCACTCTCGATTGTATGGCCTTTGTGTACAATGGAGTTTGTTACTGGTCGGGTCACCGTGATGGCTGTTTTCTGGTTCTTGGTTTGGATCCGTTTGCTGACCAGGAATGTAGTTTCACTGTATTTGATTACCCTGAAGACGAGAATTTTGTAGTGGAGTGCCCAAGCGTATCTCGAGGGGTTGTGCGGATGAGTGACTTTCACAGAGATACCAGAACTTTGTATGTTTGGGATTTGAAAGAAAAAGATGATGATTCTGTGGCTGAGGGCGGTAGCAAATTGTGTTTGAGTAAACAGGAGGTTTATTCCTTAGATCAAGAAATGTGTCCGGATGATGCACAAGTCGTTCAGAGGTTAAATTTCGACCCTAATGACAAGGACATTTTTTATCTAGAGGTGGATGGAGATATCATCATGTGCGACATTCGTACAAGAAAGTGGTCAAAGATTGTGGAAAACACTACACTCGATCGACCTTTGTGGTTCTACTTGTTCGCCCTCCCATGGTGGCCAACACCAATTCCTAGCCTACCACAGCCGGCCTCAGCTAGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

414

Amino Acids

48.0

Weight (kDa)

4.75

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 49 2.5e-06 F-box domain
b-prop_At3g26010-like PF24750 103 - 379 1.5e-14 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018397)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12920
pyrus_communis pycom09g11830 pycom09g11840
rosa_chinensis RchiOBHm_Chr5g0021011
rosa_laevigata RLG00000032577
rosa_multiflora Rmu_ssc0000244.1_g000012 Rmu_ssc0000244.1_g000015
rosa_roxburghii Rroxscaffold_1G00057420
rosa_samantha Rh5DG152500 Rh5DG152600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 449
AccB7I CCANNNNNTGG 1 cut(s) 405
AccI GTMKAC 2 cut(s) 5, 67
AccII CGCG 1 cut(s) 438
AccIII TCCGGA 1 cut(s) 1012
AciI CCGC 3 cut(s) 154, 871, 954
AclWI GGATC 3 cut(s) 578, 767, 780
AcoI YGGCCR 1 cut(s) 1193
AcsI RAATTY 7 cut(s) 57, 224, 237, 552, 572, 829, 1042
AcuI CTGAAG 2 cut(s) 612, 840
AfaI GTAC 3 cut(s) 539, 713, 1114
AfiI CCNNNNNNNGG 6 cut(s) 43, 217, 405, 430, 431, 1188
AgsI TTSAA 5 cut(s) 56, 95, 274, 610, 922
AjnI CCWGG 1 cut(s) 786
AluBI AGCT 2 cut(s) 520, 1232
AluI AGCT 2 cut(s) 520, 1232
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 1 cut(s) 626
AlwI GGATC 3 cut(s) 578, 767, 780
Ama87I CYCGRG 1 cut(s) 858
Aor13HI TCCGGA 1 cut(s) 1012
AoxI GGCC 3 cut(s) 702, 1193, 1224
ApoI RAATTY 7 cut(s) 57, 224, 237, 552, 572, 829, 1042
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
AseI ATTAAT 1 cut(s) 27
AspLEI GCGC 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 551, 731
AvaI CYCGRG 1 cut(s) 858
BaeGI GKGCMC 1 cut(s) 848
BalI TGGCCA 1 cut(s) 1195
BamHI GGATCC 1 cut(s) 772
BanI GGYRCC 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 350
BbvCI CCTCAGC 2 cut(s) 948, 1228
BccI CCATC 3 cut(s) 584, 740, 1079
BceAI ACGGC 1 cut(s) 123
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 1 cut(s) 788
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 3 cut(s) 1076, 1209, 1233
BfmI CTRYAG 1 cut(s) 532
Bme1390I CCNGG 1 cut(s) 788
BmeT110I CYCGRG 1 cut(s) 858
BmiI GGNNCC 3 cut(s) 281, 451, 774
BmrFI CCNGG 1 cut(s) 788
BmsI GCATC 1 cut(s) 1009
BpiI GAAGAC 1 cut(s) 828
BplI GAGNNNNNCTC 2 cut(s) 608, 640
BpmI CTGGAG 2 cut(s) 118, 266
Bpu10I CCTNAGC 2 cut(s) 948, 1228
BsaJI CCNNGG 1 cut(s) 1187
BsaWI WCCGGW 1 cut(s) 1012
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 217, 405, 430, 431, 1188
Bse118I RCCGGY 2 cut(s) 187, 1222
Bse1I ACTGG 3 cut(s) 311, 458, 734
Bse3DI GCAATG 1 cut(s) 410
BseAI TCCGGA 1 cut(s) 1012
BseBI CCWGG 1 cut(s) 788
BseDI CCNNGG 1 cut(s) 1187
BseGI GGATG 4 cut(s) 662, 879, 1021, 1090
BseLI CCNNNNNNNGG 6 cut(s) 43, 217, 405, 430, 431, 1188
BseMI GCAATG 1 cut(s) 410
BseMII CTCAG 3 cut(s) 30, 939, 1242
BseNI ACTGG 3 cut(s) 311, 458, 734
BseSI GKGCMC 1 cut(s) 848
Bsh1236I CGCG 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 1156
BshFI GGCC 3 cut(s) 704, 1195, 1226
BshNI GGYRCC 1 cut(s) 449
BsiEI CGRYCG 1 cut(s) 1156
BsiHKAI GWGCWC 1 cut(s) 350
BsiHKCI CYCGRG 1 cut(s) 858
BsiSI CCGG 3 cut(s) 188, 1013, 1223
BslFI GGGAC 3 cut(s) 20, 447, 477
BslI CCNNNNNNNGG 6 cut(s) 43, 217, 405, 430, 431, 1188
BsmAI GTCTC 1 cut(s) 626
BsmFI GGGAC 3 cut(s) 20, 447, 477
BsnI GGCC 3 cut(s) 704, 1195, 1226
BsoBI CYCGRG 1 cut(s) 858
Bsp1286I GDGCHC 2 cut(s) 350, 848
Bsp13I TCCGGA 1 cut(s) 1012
Bsp1407I TGTACA 1 cut(s) 711
Bsp143I GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
Bsp19I CCATGG 1 cut(s) 1187
BspACI CCGC 3 cut(s) 154, 871, 954
BspANI GGCC 3 cut(s) 704, 1195, 1226
BspCNI CTCAG 3 cut(s) 31, 940, 1241
BspEI TCCGGA 1 cut(s) 1012
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 3 cut(s) 281, 451, 774
BspPI GGATC 3 cut(s) 578, 767, 780
BspT107I GGYRCC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 410
BsrFI RCCGGY 2 cut(s) 187, 1222
BsrGI TGTACA 1 cut(s) 711
BsrI ACTGG 3 cut(s) 311, 458, 734
BssAI RCCGGY 2 cut(s) 187, 1222
BssECI CCNNGG 1 cut(s) 1187
BssMI GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
BssT1I CCWWGG 1 cut(s) 1187
Bst2UI CCWGG 1 cut(s) 788
Bst4CI ACNGT 4 cut(s) 296, 479, 743, 805
Bst6I CTCTTC 1 cut(s) 631
BstAUI TGTACA 1 cut(s) 711
BstC8I GCNNGC 2 cut(s) 113, 1224
BstDEI CTNAG 4 cut(s) 39, 948, 994, 1228
BstDSI CCRYGG 1 cut(s) 1187
BstEII GGTNACC 1 cut(s) 737
BstF5I GGATG 4 cut(s) 662, 879, 1021, 1090
BstFNI CGCG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 6 cut(s) 367, 502, 586, 775, 1000, 1156
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
BstMCI CGRYCG 1 cut(s) 1156
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 788
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 1 cut(s) 786
BstSFI CTRYAG 1 cut(s) 532
BstSLI GKGCMC 1 cut(s) 848
BstUI CGCG 1 cut(s) 438
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 772
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 1 cut(s) 772
BsuRI GGCC 3 cut(s) 704, 1195, 1226
BtgI CCRYGG 1 cut(s) 1187
BtsCI GGATG 4 cut(s) 662, 879, 1021, 1090
BtsIMutI CAGTG 2 cut(s) 465, 801
Cac8I GCNNGC 2 cut(s) 113, 1224
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 2 cut(s) 187, 1222
CseI GACGC 2 cut(s) 152, 444
Csp6I GTAC 3 cut(s) 538, 712, 1113
CspCI CAANNNNNGTGG 2 cut(s) 1116, 1151
CviAII CATG 3 cut(s) 454, 1099, 1188
CviQI GTAC 3 cut(s) 538, 712, 1113
DdeI CTNAG 4 cut(s) 39, 948, 994, 1228
DpnI GATC 6 cut(s) 366, 501, 585, 774, 999, 1155
DpnII GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
EaeI YGGCCR 1 cut(s) 1193
Eam1104I CTCTTC 1 cut(s) 631
EarI CTCTTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 1187
Eco32I GATATC 1 cut(s) 1093
Eco57I CTGAAG 2 cut(s) 612, 840
Eco88I CYCGRG 1 cut(s) 858
Eco91I GGTNACC 1 cut(s) 737
EcoO65I GGTNACC 1 cut(s) 737
EcoRI GAATTC 3 cut(s) 224, 237, 552
EcoRII CCWGG 1 cut(s) 786
EcoRV GATATC 1 cut(s) 1093
EcoT14I CCWWGG 1 cut(s) 1187
ErhI CCWWGG 1 cut(s) 1187
FaeI CATG 3 cut(s) 457, 1102, 1191
FaqI GGGAC 3 cut(s) 20, 447, 477
FatI CATG 3 cut(s) 453, 1098, 1187
FauNDI CATATG 2 cut(s) 267, 383
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 2 cut(s) 5, 67
FokI GGATG 4 cut(s) 649, 886, 1028, 1097
FspBI CTAG 3 cut(s) 1076, 1209, 1233
GlaI GCGC 1 cut(s) 79
GsuI CTGGAG 2 cut(s) 118, 266
HaeIII GGCC 3 cut(s) 704, 1195, 1226
HapII CCGG 3 cut(s) 188, 1013, 1223
HgaI GACGC 2 cut(s) 152, 444
HhaI GCGC 1 cut(s) 80
Hin1II CATG 3 cut(s) 457, 1102, 1191
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 3 cut(s) 6, 68, 151
HindII GTYRAC 3 cut(s) 6, 68, 151
HinfI GANTC 3 cut(s) 41, 465, 938
HpaI GTTAAC 1 cut(s) 151
HpaII CCGG 3 cut(s) 188, 1013, 1223
HphI GGTGA 2 cut(s) 551, 731
Hpy166II GTNNAC 6 cut(s) 6, 68, 123, 151, 712, 977
Hpy188I TCNGA 4 cut(s) 253, 504, 631, 1035
Hpy188III TCNNGA 7 cut(s) 283, 587, 692, 858, 1001, 1013, 1076
Hpy8I GTNNAC 6 cut(s) 6, 68, 123, 151, 712, 977
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 4 cut(s) 296, 479, 743, 805
HpyCH4V TGCA 7 cut(s) 99, 111, 169, 198, 292, 387, 1022
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 4 cut(s) 39, 948, 994, 1228
Hsp92II CATG 3 cut(s) 457, 1102, 1191
HspAI GCGC 1 cut(s) 78
Kpn2I TCCGGA 1 cut(s) 1012
KroI GCCGGC 1 cut(s) 1222
KroNI GCCGGC 1 cut(s) 1224
Ksp22I TGATCA 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 151
Kzo9I GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
LmnI GCTCC 2 cut(s) 285, 517
LweI GCATC 1 cut(s) 1009
MaeI CTAG 3 cut(s) 1076, 1209, 1233
MaeIII GTNAC 3 cut(s) 725, 737, 878
MalI GATC 6 cut(s) 366, 501, 585, 774, 999, 1155
MboI GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
MboII GAAGA 3 cut(s) 548, 618, 833
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 2 cut(s) 350, 848
MlsI TGGCCA 1 cut(s) 1195
MluNI TGGCCA 1 cut(s) 1195
MlyI GAGTC 2 cut(s) 50, 459
MmeI TCCRAC 1 cut(s) 231
Mox20I TGGCCA 1 cut(s) 1195
MroI TCCGGA 1 cut(s) 1012
MroNI GCCGGC 1 cut(s) 1222
MscI TGGCCA 1 cut(s) 1195
MseI TTAA 5 cut(s) 27, 150, 522, 576, 1040
MslI CAYNNNNRTG 3 cut(s) 458, 564, 744
Msp20I TGGCCA 1 cut(s) 1195
MspI CCGG 3 cut(s) 188, 1013, 1223
MspR9I CCNGG 1 cut(s) 788
MvaI CCWGG 1 cut(s) 788
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 1 cut(s) 354
NaeI GCCGGC 1 cut(s) 1224
NcoI CCATGG 1 cut(s) 1187
NdeI CATATG 2 cut(s) 267, 383
NdeII GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
NgoMIV GCCGGC 1 cut(s) 1222
NlaIII CATG 3 cut(s) 457, 1102, 1191
NlaIV GGNNCC 3 cut(s) 281, 451, 774
NmuCI GTSAC 2 cut(s) 737, 878
PaeR7I CTCGAG 1 cut(s) 858
PdiI GCCGGC 1 cut(s) 1224
PfeI GAWTC 1 cut(s) 938
PflMI CCANNNNNTGG 1 cut(s) 405
Ple19I CGATCG 1 cut(s) 1156
PleI GAGTC 2 cut(s) 49, 459
PpsI GAGTC 2 cut(s) 49, 459
PshBI ATTAAT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 786
PspEI GGTNACC 1 cut(s) 737
PspGI CCWGG 1 cut(s) 786
PspN4I GGNNCC 3 cut(s) 281, 451, 774
PsuI RGATCY 1 cut(s) 772
PvuI CGATCG 1 cut(s) 1156
RsaI GTAC 3 cut(s) 539, 713, 1114
RsaNI GTAC 3 cut(s) 538, 712, 1113
RseI CAYNNNNRTG 3 cut(s) 458, 564, 744
SalI GTCGAC 2 cut(s) 4, 66
SaqAI TTAA 5 cut(s) 27, 150, 522, 576, 1040
Sau3AI GATC 6 cut(s) 364, 499, 583, 772, 997, 1153
SchI GAGTC 2 cut(s) 50, 459
ScrFI CCNGG 1 cut(s) 788
SduI GDGCHC 2 cut(s) 350, 848
SfaNI GCATC 1 cut(s) 1009
SfcI CTRYAG 1 cut(s) 532
Sfr274I CTCGAG 1 cut(s) 858
SlaI CTCGAG 1 cut(s) 858
SmiMI CAYNNNNRTG 3 cut(s) 458, 564, 744
SmlI CTYRAG 1 cut(s) 858
SmoI CTYRAG 1 cut(s) 858
SsiI CCGC 3 cut(s) 154, 871, 954
SspMI CTAG 3 cut(s) 1076, 1209, 1233
StyD4I CCNGG 1 cut(s) 786
StyI CCWWGG 1 cut(s) 1187
TaaI ACNGT 4 cut(s) 296, 479, 743, 805
TaqI TCGA 7 cut(s) 5, 67, 693, 859, 1047, 1152, 1156
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 1 cut(s) 938
Tru1I TTAA 5 cut(s) 27, 150, 522, 576, 1040
Tru9I TTAA 5 cut(s) 27, 150, 522, 576, 1040
TscAI CASTG 2 cut(s) 465, 808
TseFI GTSAC 2 cut(s) 737, 878
Tsp45I GTSAC 2 cut(s) 737, 878
TspDTI ATGAA 3 cut(s) 29, 76, 585
TspGWI ACGGA 1 cut(s) 765
TspRI CASTG 2 cut(s) 465, 808
Van91I CCANNNNNTGG 1 cut(s) 405
VspI ATTAAT 1 cut(s) 27
XapI RAATTY 7 cut(s) 57, 224, 237, 552, 572, 829, 1042
XbaI TCTAGA 1 cut(s) 1075
XhoI CTCGAG 1 cut(s) 858
XmiI GTMKAC 2 cut(s) 5, 67
XspI CTAG 3 cut(s) 1076, 1209, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.