Rh5DG152600

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018397
Physical Location & Seq
Forward (+)
15521022 .. 15522412
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG152600.1

Sequence Viewer

Length: 849 bp
ATGGGGAGCCCAATGCCAAAATTCTCTCCTGCTGGATTCGGCCTCTCAACTCCAGAAGATGAAGGGAAGAGGCAGATCATACAGCTTAATGCGGACTACAAGTGCAAAGTTATCAGACTTCTTAGCTCTTATGAGTATCCTAAGAACTACAGGTGCAAGGTGGAGATCTTCTCTTCCGAGACCGGTGAATGGAGCAAGACAGTTATAATTTCCCCTAAACCTTGGTATCATATCTATAGCAGTATTGCTCACAATGGAGTGTTGTATCACTTGTGTCAATCAACTGATTCTAGGACCTGTCTTGCTGGGTTGGATCCTTTCATGATAATGAACAAAATCACGAACAGTACTCCTGGCAATGGTGTTGATCATTATAAATGTTGTTATGTAGAAATGGATACAATTGTGGTAGGCGGCTTCAGTGGAGATTATTGTGTTGTCAGTGAACGGTCTCTGCGGATTTCCTACTCTGACTGGGATACATTCAGTGTATATGTTTGGGACTTAAAAGAAGAAGAAGAAGGTCATGCTGAGGTGGTCATAGAAGGAACTGGAAAACTGTGTTGGAAACACACGGGGGTATATTCACTAGACCGAGATATAGCTCCAGATGAATCATCAGTATCAGTGTTTGAGCTGATAGCTATCGATCCAAATAATGATGATATATTGTATCTAAATATTAATGGAGACCTTGTGATGAGCAATATTCAAACAAGAAAGTGGTCAGTGCTAGTTAAAGAGAGTCAAATCATCGATTCTCACTTCTTCCAGTTGCAGCTTCCATTGTGGCCAACACCAATTCCTAAACTACCGCAGCATGCCAAAGTTGGAGGAACTAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

282

Amino Acids

31.8

Weight (kDa)

5.26

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 36 - 253 1.1e-09 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018397)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12920
pyrus_communis pycom09g11830 pycom09g11840
rosa_chinensis RchiOBHm_Chr5g0021011
rosa_laevigata RLG00000032577
rosa_multiflora Rmu_ssc0000244.1_g000012 Rmu_ssc0000244.1_g000015
rosa_roxburghii Rroxscaffold_1G00057420
rosa_samantha Rh5DG152500 Rh5DG152600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 206, 375
AciI CCGC 4 cut(s) 92, 414, 457, 815
AclWI GGATC 3 cut(s) 308, 321, 644
AcoI YGGCCR 1 cut(s) 791
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 403
AfaI GTAC 1 cut(s) 349
AfiI CCNNNNNNNGG 2 cut(s) 189, 359
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 1 cut(s) 713
AjnI CCWGG 1 cut(s) 352
AjuI GAANNNNNNNTTGG 2 cut(s) 547, 579
AluBI AGCT 7 cut(s) 85, 126, 605, 637, 644, 781, 846
AluI AGCT 7 cut(s) 85, 126, 605, 637, 644, 781, 846
Alw26I GTCTC 3 cut(s) 173, 456, 684
AlwI GGATC 3 cut(s) 308, 321, 644
AoxI GGCC 2 cut(s) 40, 791
ApeKI GCWGC 3 cut(s) 778, 817, 843
ApoI RAATTY 1 cut(s) 20
AseI ATTAAT 1 cut(s) 684
AsiGI ACCGGT 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 1 cut(s) 294
BaeI ACNNNNGTAYC 4 cut(s) 209, 242, 390, 423
BalI TGGCCA 1 cut(s) 793
BamHI GGATCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 11
BbvCI CCTCAGC 1 cut(s) 531
BbvI GCAGC 2 cut(s) 790, 829
BciT130I CCWGG 1 cut(s) 354
BciVI GTATCC 3 cut(s) 147, 391, 472
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 3 cut(s) 173, 456, 684
BfaI CTAG 5 cut(s) 291, 590, 734, 840, 847
BfmI CTRYAG 2 cut(s) 148, 235
BfuI GTATCC 3 cut(s) 147, 391, 472
BglII AGATCT 1 cut(s) 165
BisI GCNGC 4 cut(s) 415, 779, 818, 844
BlsI GCNGC 4 cut(s) 416, 780, 819, 845
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 294
BmiI GGNNCC 2 cut(s) 8, 315
BmrFI CCNGG 1 cut(s) 354
BmrI ACTGGG 1 cut(s) 484
BmuI ACTGGG 1 cut(s) 484
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpmI CTGGAG 2 cut(s) 36, 591
Bpu10I CCTNAGC 1 cut(s) 531
Bsa29I ATCGAT 2 cut(s) 648, 756
BsaBI GATNNNNATC 1 cut(s) 644
BsaI GGTCTC 3 cut(s) 173, 456, 684
BsaJI CCNNGG 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 359
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 479, 556, 772
Bse3DI GCAATG 1 cut(s) 364
Bse8I GATNNNNATC 1 cut(s) 644
BseBI CCWGG 1 cut(s) 354
BseCI ATCGAT 2 cut(s) 648, 756
BseDI CCNNGG 1 cut(s) 221
BseJI GATNNNNATC 1 cut(s) 644
BseLI CCNNNNNNNGG 2 cut(s) 189, 359
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 3 cut(s) 479, 556, 772
BseXI GCAGC 2 cut(s) 790, 829
BseYI CCCAGC 1 cut(s) 305
BshFI GGCC 2 cut(s) 42, 793
BshTI ACCGGT 1 cut(s) 182
BshVI ATCGAT 2 cut(s) 648, 756
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 2 cut(s) 189, 359
BsmAI GTCTC 3 cut(s) 173, 456, 684
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 2 cut(s) 42, 793
Bso31I GGTCTC 3 cut(s) 173, 456, 684
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 5 cut(s) 75, 165, 313, 367, 649
BspACI CCGC 4 cut(s) 92, 414, 457, 815
BspANI GGCC 2 cut(s) 42, 793
BspCNI CTCAG 1 cut(s) 523
BspDI ATCGAT 2 cut(s) 648, 756
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 2 cut(s) 8, 315
BspPI GGATC 3 cut(s) 308, 321, 644
BspTNI GGTCTC 3 cut(s) 173, 456, 684
BsrDI GCAATG 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 479, 556, 772
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 5 cut(s) 75, 165, 313, 367, 649
BssT1I CCWWGG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 4 cut(s) 202, 347, 450, 561
Bst6I CTCTTC 2 cut(s) 62, 178
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 3 cut(s) 122, 141, 531
BstKTI GATC 5 cut(s) 78, 168, 316, 370, 652
BstMAI GTCTC 3 cut(s) 173, 456, 684
BstMBI GATC 5 cut(s) 75, 165, 313, 367, 649
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 824
BstSCI CCNGG 1 cut(s) 352
BstSFI CTRYAG 2 cut(s) 148, 235
BstV1I GCAGC 2 cut(s) 790, 829
BstX2I RGATCY 2 cut(s) 165, 313
BstYI RGATCY 2 cut(s) 165, 313
Bsu15I ATCGAT 2 cut(s) 648, 756
BsuI GTATCC 3 cut(s) 147, 391, 472
BsuRI GGCC 2 cut(s) 42, 793
BsuTUI ATCGAT 2 cut(s) 648, 756
BtsIMutI CAGTG 5 cut(s) 427, 448, 493, 633, 735
Cac8I GCNNGC 1 cut(s) 822
CciI TCATGA 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 294
ClaI ATCGAT 2 cut(s) 648, 756
Csp6I GTAC 1 cut(s) 348
CspAI ACCGGT 1 cut(s) 182
CviAII CATG 3 cut(s) 322, 527, 821
CviQI GTAC 1 cut(s) 348
DdeI CTNAG 3 cut(s) 122, 141, 531
DpnI GATC 5 cut(s) 77, 167, 315, 369, 651
DpnII GATC 5 cut(s) 75, 165, 313, 367, 649
EaeI YGGCCR 1 cut(s) 791
Eam1104I CTCTTC 2 cut(s) 62, 178
EarI CTCTTC 2 cut(s) 62, 178
Eco130I CCWWGG 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 3 cut(s) 173, 456, 684
Eco47I GGWCC 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 221
FaeI CATG 3 cut(s) 325, 530, 824
FaqI GGGAC 1 cut(s) 515
FatI CATG 3 cut(s) 321, 526, 820
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 4 cut(s) 415, 779, 818, 844
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 4 cut(s) 415, 779, 818, 844
FspBI CTAG 5 cut(s) 291, 590, 734, 840, 847
GluI GCNGC 4 cut(s) 415, 779, 818, 844
GsaI CCCAGC 1 cut(s) 309
GsuI CTGGAG 2 cut(s) 36, 591
HaeIII GGCC 2 cut(s) 42, 793
HapII CCGG 1 cut(s) 183
Hin1II CATG 3 cut(s) 325, 530, 824
HinfI GANTC 5 cut(s) 36, 287, 614, 745, 758
HpaII CCGG 1 cut(s) 183
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 3 cut(s) 116, 178, 472
Hpy188III TCNNGA 4 cut(s) 53, 322, 340, 608
Hpy8I GTNNAC 1 cut(s) 446
HpyAV CCTTC 3 cut(s) 56, 515, 539
HpyCH4III ACNGT 4 cut(s) 202, 347, 450, 561
HpyCH4V TGCA 3 cut(s) 105, 156, 778
HpyF3I CTNAG 3 cut(s) 122, 141, 531
Hsp92II CATG 3 cut(s) 325, 530, 824
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 5 cut(s) 75, 165, 313, 367, 649
LmnI GCTCC 3 cut(s) 6, 192, 610
Lsp1109I GCAGC 2 cut(s) 790, 829
MaeI CTAG 5 cut(s) 291, 590, 734, 840, 847
MalI GATC 5 cut(s) 77, 167, 315, 369, 651
MboI GATC 5 cut(s) 75, 165, 313, 367, 649
MboII GAAGA 8 cut(s) 68, 79, 160, 165, 524, 527, 530, 760
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 2 cut(s) 165, 313
MhlI GDGCHC 1 cut(s) 11
MlsI TGGCCA 1 cut(s) 793
MluCI AATT 4 cut(s) 20, 207, 402, 801
MluNI TGGCCA 1 cut(s) 793
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 3 cut(s) 291, 545, 811
MnlI CCTC 4 cut(s) 53, 63, 526, 827
Mox20I TGGCCA 1 cut(s) 793
MscI TGGCCA 1 cut(s) 793
MseI TTAA 4 cut(s) 87, 506, 684, 738
MslI CAYNNNNRTG 1 cut(s) 326
Msp20I TGGCCA 1 cut(s) 793
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 1 cut(s) 354
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 354
NdeII GATC 5 cut(s) 75, 165, 313, 367, 649
NlaIII CATG 3 cut(s) 325, 530, 824
NlaIV GGNNCC 2 cut(s) 8, 315
NspI RCATGY 1 cut(s) 824
PaeI GCATGC 1 cut(s) 824
PagI TCATGA 1 cut(s) 321
PfeI GAWTC 4 cut(s) 36, 287, 614, 758
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 4 cut(s) 416, 780, 819, 845
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
PpuMI RGGWCCY 1 cut(s) 294
PshBI ATTAAT 1 cut(s) 684
PsiI TTATAA 2 cut(s) 206, 375
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 2 cut(s) 8, 315
PspPI GGNCC 1 cut(s) 294
PspPPI RGGWCCY 1 cut(s) 294
PsuI RGATCY 2 cut(s) 165, 313
RsaI GTAC 1 cut(s) 349
RsaNI GTAC 1 cut(s) 348
RseI CAYNNNNRTG 1 cut(s) 326
SaqAI TTAA 4 cut(s) 87, 506, 684, 738
SatI GCNGC 4 cut(s) 415, 779, 818, 844
Sau3AI GATC 5 cut(s) 75, 165, 313, 367, 649
Sau96I GGNCC 1 cut(s) 294
ScaI AGTACT 1 cut(s) 349
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 1 cut(s) 11
SfcI CTRYAG 2 cut(s) 148, 235
SinI GGWCC 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 326
SphI GCATGC 1 cut(s) 824
Sse9I AATT 4 cut(s) 20, 207, 402, 801
SsiI CCGC 4 cut(s) 92, 414, 457, 815
SspI AATATT 2 cut(s) 682, 709
SspMI CTAG 5 cut(s) 291, 590, 734, 840, 847
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 4 cut(s) 202, 347, 450, 561
TaqI TCGA 2 cut(s) 648, 756
TaqII GACCGA 1 cut(s) 609
TasI AATT 4 cut(s) 20, 207, 402, 801
TatI WGTACW 1 cut(s) 347
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 4 cut(s) 36, 287, 614, 758
Tru1I TTAA 4 cut(s) 87, 506, 684, 738
Tru9I TTAA 4 cut(s) 87, 506, 684, 738
TscAI CASTG 5 cut(s) 427, 448, 493, 633, 735
TseI GCWGC 3 cut(s) 778, 817, 843
TspDTI ATGAA 4 cut(s) 75, 310, 344, 627
TspRI CASTG 5 cut(s) 427, 448, 493, 633, 735
VpaK11BI GGWCC 1 cut(s) 294
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 824
XspI CTAG 5 cut(s) 291, 590, 734, 840, 847
ZrmI AGTACT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.