pycom09g12140

non-haem dioxygenase in morphine synthesis N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0014284
Physical Location & Seq
Forward (+)
10581966 .. 10584222
2257 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGATATGTCATTGTGCACAAGAACTAGAACAAGTGAGTTTATTCCATCACTCATCTCTCTACTTTCCAATCCCAATGGCAACCTCCCCGGAACCAATCTCCGAAAACCCTGTTGATTTTCGTGCCCCGCCGCCTTCTCCCGTTGCCTCTGGTCGCCTATCATCAGTCGCAAACGATGAAGTCCTCACCGAATTCCTCGAGCACTCCCTCCAAGTCCCTGACCTCATCTTGCCAGACAAGTTTTTCCCCAAGCAAATATCTATTGAAAACAATCCTCCCACAATTGATTTTGGAGCCCTGAATTCGGATGGATGTGACCCTAGTCTTTGCAAGGTGGTGGAGTCCATAGCAAGAAATGGATGCTTTCAGCTGGTGAACCATGGAATTTCAAGTGAGCTTGTGAGGCCAGTGCAGGCTGCCGCCGCGGGAATTTTTAGCGTGTCACCGGAGACGAGGGCTGAGGTTACTAGGTCACCGGAGAAGCCATACGGGTTCGAGGAAGTTCATGCTGAGGAGGCTGACCATGTATTGAGTGAAGAGTTTGTTTGGTGTAGAGACCAAGGCTTGAAGTTGGCAATGGATGGAATTGCCCCAATTGGATATTCAAATTTCAGTGAAAAACTAGAGACCCTTATGACAAATATTGAGAAAGTCTGTGAGAAAATTCTACTAAGCTTGTTGATTAATTCTCAAGAAAAAATCATTCATGGAGAAAGCAAAAATGATGAAACGGTGCAAAGGAAAGAAGTTGGGACAGTTTGTTGCTTGTACAAGCATAGCCAGAATGTGTTACAAGATGAATGGGATAAGTCTCTGAGATATGATGTGATTAAGATGCTGATTAGAGGGACTGATTACTCTCATGCCTTGTGTTTACATATCTGTGATGGTTCTTCAGAGTTCCATGTTTACTCCAAGAAAGGTTGGGTTTCTTTTATCCCAGTTAAAAATGCCCTAATAGTCACCACTGGGGACCAAATACAGGCATGGAGTGGCAGCCAGTATAAGCATGTGATTGGAAGGCCAATCTTTAAAGGTGAGGAGGAATTTATTTCAGTGGCTTTCCTCTATTCTCCTCCAAGCTTCATTACAAACTCCCAAAGCAGCAAGGAAAATACTATTTCACTTGGTCAACAAGTTATAGTGGCTATACTTCTTACTCTTGTCTATCAATTTTCAGTATACTTGTCAAGCCATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

401

Amino Acids

44.57

Weight (kDa)

5.15

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 93 - 175 1.8e-11 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79760 AT1G79760
fragaria_vesca FvH4_6g32300 FvH4_6g32300
malus_domestica MD09G1204500.v1.1
prunus_persica Prupe.3G060100_v2.0.a1
pyrus_communis pycom09g12140
rosa_chinensis RchiOBHm_Chr2g0140341
rosa_laevigata RLG00000019860
rosa_multiflora Rmu_sc0005446.1_g000032
rosa_roxburghii Rroxscaffold_2G00104920
rosa_rugosa Rorug02G0361700
rosa_samantha Rh2AG413000 Rh2BG423400 Rh2CG399000 Rh2DG432300
rosa_wichuraiana Rw2G033850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1182
AccII CGCG 1 cut(s) 425
AciI CCGC 5 cut(s) 128, 131, 420, 423, 425
AcsI RAATTY 7 cut(s) 191, 301, 384, 429, 607, 663, 1046
AcuI CTGAAG 1 cut(s) 879
AfaI GTAC 1 cut(s) 770
AfiI CCNNNNNNNGG 2 cut(s) 304, 982
AgsI TTSAA 4 cut(s) 266, 390, 568, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 586, 618
AluBI AGCT 4 cut(s) 370, 397, 675, 1083
AluI AGCT 4 cut(s) 370, 397, 675, 1083
Alw21I GWGCWC 2 cut(s) 19, 204
Alw26I GTCTC 4 cut(s) 443, 549, 620, 816
Alw44I GTGCAC 1 cut(s) 15
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 404, 1022
ApaLI GTGCAC 1 cut(s) 15
ApeKI GCWGC 3 cut(s) 416, 996, 1104
ApoI RAATTY 7 cut(s) 191, 301, 384, 429, 607, 663, 1046
AseI ATTAAT 1 cut(s) 684
AspS9I GGNCC 1 cut(s) 973
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 6 cut(s) 178, 385, 435, 465, 955, 1049
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 1 cut(s) 973
BaeGI GKGCMC 2 cut(s) 19, 127
BanII GRGCYC 1 cut(s) 298
Bbv12I GWGCWC 2 cut(s) 19, 204
BbvCI CCTCAGC 2 cut(s) 459, 510
BbvI GCAGC 3 cut(s) 403, 1008, 1116
BccI CCATC 4 cut(s) 54, 302, 575, 881
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 4 cut(s) 443, 549, 620, 816
BfaI CTAG 4 cut(s) 26, 321, 468, 623
BisI GCNGC 6 cut(s) 131, 417, 420, 423, 997, 1105
BlsI GCNGC 6 cut(s) 132, 418, 421, 424, 998, 1106
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 973
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 973
BmiI GGNNCC 3 cut(s) 93, 295, 974
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 2 cut(s) 935, 978
BmsI GCATC 2 cut(s) 350, 825
BmuI ACTGGG 2 cut(s) 935, 978
BoxI GACNNNNGTC 1 cut(s) 321
Bpu10I CCTNAGC 2 cut(s) 459, 510
BpuEI CTTGAG 1 cut(s) 675
BpuMI CCSGG 1 cut(s) 89
BsaI GGTCTC 2 cut(s) 549, 620
BsaJI CCNNGG 4 cut(s) 87, 379, 423, 559
BsaWI WCCGGW 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 982
Bse1I ACTGG 4 cut(s) 407, 941, 973, 1000
Bse3DI GCAATG 1 cut(s) 582
BseDI CCNNGG 4 cut(s) 87, 379, 423, 559
BseGI GGATG 4 cut(s) 313, 317, 365, 586
BseLI CCNNNNNNNGG 2 cut(s) 304, 982
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 3 cut(s) 450, 501, 806
BseNI ACTGG 4 cut(s) 407, 941, 973, 1000
BseRI GAGGAG 3 cut(s) 527, 1055, 1065
BseSI GKGCMC 2 cut(s) 19, 127
BseXI GCAGC 3 cut(s) 403, 1008, 1116
BsgI GTGCAG 1 cut(s) 431
Bsh1236I CGCG 1 cut(s) 425
BshFI GGCC 2 cut(s) 406, 1024
BsiHKAI GWGCWC 2 cut(s) 19, 204
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 3 cut(s) 89, 446, 476
BslFI GGGAC 4 cut(s) 200, 766, 862, 986
BslI CCNNNNNNNGG 2 cut(s) 304, 982
BsmAI GTCTC 4 cut(s) 443, 549, 620, 816
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 4 cut(s) 200, 766, 862, 986
BsnI GGCC 2 cut(s) 406, 1024
Bso31I GGTCTC 2 cut(s) 549, 620
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 4 cut(s) 19, 127, 204, 298
Bsp1407I TGTACA 1 cut(s) 768
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 5 cut(s) 128, 131, 420, 423, 425
BspANI GGCC 2 cut(s) 406, 1024
BspCNI CTCAG 3 cut(s) 451, 502, 807
BspFNI CGCG 1 cut(s) 425
BspLI GGNNCC 3 cut(s) 93, 295, 974
BspTNI GGTCTC 2 cut(s) 549, 620
BsrDI GCAATG 1 cut(s) 582
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 4 cut(s) 407, 941, 973, 1000
BssECI CCNNGG 4 cut(s) 87, 379, 423, 559
BssNAI GTATAC 1 cut(s) 1183
BssT1I CCWWGG 2 cut(s) 379, 559
Bst1107I GTATAC 1 cut(s) 1183
Bst4CI ACNGT 2 cut(s) 733, 757
Bst6I CTCTTC 1 cut(s) 531
BstAUI TGTACA 1 cut(s) 768
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 4 cut(s) 459, 510, 671, 815
BstDSI CCRYGG 2 cut(s) 379, 423
BstEII GGTNACC 1 cut(s) 471
BstF5I GGATG 4 cut(s) 313, 317, 365, 586
BstFNI CGCG 1 cut(s) 425
BstMAI GTCTC 4 cut(s) 443, 549, 620, 816
BstMWI GCNNNNNNNGC 3 cut(s) 403, 422, 515
BstNSI RCATGY 1 cut(s) 1013
BstPAI GACNNNNGTC 1 cut(s) 321
BstPI GGTNACC 1 cut(s) 471
BstSCI CCNGG 1 cut(s) 87
BstSLI GKGCMC 2 cut(s) 19, 127
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 3 cut(s) 403, 1008, 1116
BstZ17I GTATAC 1 cut(s) 1183
BsuRI GGCC 2 cut(s) 406, 1024
BtgI CCRYGG 2 cut(s) 379, 423
BtsCI GGATG 4 cut(s) 313, 317, 365, 586
BtsIMutI CAGTG 4 cut(s) 414, 619, 966, 1062
Cac8I GCNNGC 1 cut(s) 414
Cfr13I GGNCC 1 cut(s) 973
Cfr42I CCGCGG 1 cut(s) 426
Csp6I GTAC 1 cut(s) 769
CviAII CATG 8 cut(s) 380, 506, 524, 707, 863, 905, 987, 1010
CviQI GTAC 1 cut(s) 769
DdeI CTNAG 4 cut(s) 459, 510, 671, 815
DraI TTTAAA 1 cut(s) 1033
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco130I CCWWGG 2 cut(s) 379, 559
Eco24I GRGCYC 1 cut(s) 298
Eco31I GGTCTC 2 cut(s) 549, 620
Eco47I GGWCC 1 cut(s) 973
Eco57I CTGAAG 1 cut(s) 879
Eco88I CYCGRG 1 cut(s) 197
Eco91I GGTNACC 1 cut(s) 471
EcoO65I GGTNACC 1 cut(s) 471
EcoRI GAATTC 2 cut(s) 191, 301
EcoT14I CCWWGG 2 cut(s) 379, 559
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 2 cut(s) 379, 559
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 8 cut(s) 383, 509, 527, 710, 866, 908, 990, 1013
FaqI GGGAC 4 cut(s) 200, 766, 862, 986
FatI CATG 8 cut(s) 379, 505, 523, 706, 862, 904, 986, 1009
FauI CCCGC 2 cut(s) 135, 418
FblI GTMKAC 1 cut(s) 1182
Fnu4HI GCNGC 6 cut(s) 131, 417, 420, 423, 997, 1105
FokI GGATG 4 cut(s) 320, 324, 372, 593
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 6 cut(s) 131, 417, 420, 423, 997, 1105
FspBI CTAG 4 cut(s) 26, 321, 468, 623
GluI GCNGC 6 cut(s) 131, 417, 420, 423, 997, 1105
HaeIII GGCC 2 cut(s) 406, 1024
HapII CCGG 3 cut(s) 89, 446, 476
Hin1II CATG 8 cut(s) 383, 509, 527, 710, 866, 908, 990, 1013
HincII GTYRAC 1 cut(s) 1133
HindII GTYRAC 1 cut(s) 1133
HindIII AAGCTT 2 cut(s) 673, 1081
HinfI GANTC 1 cut(s) 341
HpaII CCGG 3 cut(s) 89, 446, 476
HphI GGTGA 6 cut(s) 178, 385, 435, 465, 955, 1049
Hpy166II GTNNAC 6 cut(s) 17, 376, 875, 910, 1133, 1183
Hpy188I TCNGA 5 cut(s) 103, 307, 816, 898, 1202
Hpy188III TCNNGA 1 cut(s) 692
Hpy8I GTNNAC 6 cut(s) 17, 376, 875, 910, 1133, 1183
HpyAV CCTTC 2 cut(s) 144, 1014
HpyCH4III ACNGT 2 cut(s) 733, 757
HpyCH4V TGCA 4 cut(s) 17, 330, 412, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 403, 422, 515
HpyF3I CTNAG 4 cut(s) 459, 510, 671, 815
Hsp92II CATG 8 cut(s) 383, 509, 527, 710, 866, 908, 990, 1013
KspI CCGCGG 1 cut(s) 426
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 3 cut(s) 403, 1008, 1116
LweI GCATC 2 cut(s) 350, 825
MaeI CTAG 4 cut(s) 26, 321, 468, 623
MaeIII GTNAC 6 cut(s) 314, 441, 463, 471, 789, 961
MboII GAAGA 2 cut(s) 548, 885
MfeI CAATTG 2 cut(s) 282, 594
MhlI GDGCHC 4 cut(s) 19, 127, 204, 298
MlyI GAGTC 1 cut(s) 350
MseI TTAA 4 cut(s) 684, 831, 945, 1032
MslI CAYNNNNRTG 1 cut(s) 882
MspA1I CMGCKG 2 cut(s) 370, 425
MspI CCGG 3 cut(s) 89, 446, 476
MspR9I CCNGG 1 cut(s) 89
MunI CAATTG 2 cut(s) 282, 594
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 3 cut(s) 403, 422, 515
NciI CCSGG 1 cut(s) 89
NcoI CCATGG 1 cut(s) 379
NlaIII CATG 8 cut(s) 383, 509, 527, 710, 866, 908, 990, 1013
NlaIV GGNNCC 3 cut(s) 93, 295, 974
NmuCI GTSAC 4 cut(s) 314, 441, 471, 961
NspI RCATGY 1 cut(s) 1013
PaeR7I CTCGAG 1 cut(s) 197
PkrI GCNGC 6 cut(s) 132, 418, 421, 424, 998, 1106
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PshAI GACNNNNGTC 1 cut(s) 321
PshBI ATTAAT 1 cut(s) 684
PspEI GGTNACC 1 cut(s) 471
PspN4I GGNNCC 3 cut(s) 93, 295, 974
PspPI GGNCC 1 cut(s) 973
PspXI VCTCGAGB 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 370
RsaI GTAC 1 cut(s) 770
RsaNI GTAC 1 cut(s) 769
RseI CAYNNNNRTG 1 cut(s) 882
SacII CCGCGG 1 cut(s) 426
SaqAI TTAA 4 cut(s) 684, 831, 945, 1032
SatI GCNGC 6 cut(s) 131, 417, 420, 423, 997, 1105
Sau96I GGNCC 1 cut(s) 973
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 4 cut(s) 19, 127, 204, 298
SfaNI GCATC 2 cut(s) 350, 825
Sfr274I CTCGAG 1 cut(s) 197
Sfr303I CCGCGG 1 cut(s) 426
SgrBI CCGCGG 1 cut(s) 426
SinI GGWCC 1 cut(s) 973
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 882
SmlI CTYRAG 2 cut(s) 197, 690
SmoI CTYRAG 2 cut(s) 197, 690
SsiI CCGC 5 cut(s) 128, 131, 420, 423, 425
SspI AATATT 1 cut(s) 643
SspMI CTAG 4 cut(s) 26, 321, 468, 623
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 2 cut(s) 379, 559
TaaI ACNGT 2 cut(s) 733, 757
TaqI TCGA 2 cut(s) 198, 495
TatI WGTACW 1 cut(s) 768
TauI GCSGC 3 cut(s) 133, 422, 425
Tru1I TTAA 4 cut(s) 684, 831, 945, 1032
Tru9I TTAA 4 cut(s) 684, 831, 945, 1032
TscAI CASTG 4 cut(s) 414, 619, 973, 1062
TseFI GTSAC 4 cut(s) 314, 441, 471, 961
TseI GCWGC 3 cut(s) 416, 996, 1104
Tsp45I GTSAC 4 cut(s) 314, 441, 471, 961
TspDTI ATGAA 6 cut(s) 192, 494, 695, 741, 813, 1075
TspRI CASTG 4 cut(s) 414, 619, 973, 1062
VneI GTGCAC 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 973
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 7 cut(s) 191, 301, 384, 429, 607, 663, 1046
XceI RCATGY 1 cut(s) 1013
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 1182
XspI CTAG 4 cut(s) 26, 321, 468, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.