pycom09g15600

Protein LIGHT-DEPENDENT SHORT HYPOCOTYLS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0009757
Physical Location & Seq
Reverse (-)
15556501 .. 15557046
546 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g15600.1

Sequence Viewer

Length: 546 bp
ATGTCTGGAGAAAGAGGCAGAGATTTCGCCGAAGGATCGTCCGCTGGAGGTTCTCTGAATGATGATCAGCAGCAGCCGGCCACACCAAGCCGCTATGAATCCCAAAAGAGAAGGGACTGGAACACTTTTGGTCAGTACTTGAAGAACCAGAGGCCTCCAGTGGCGCTGTCTCAGAGCAATTACAACCATGTACTGGAGTTTCTTAGGTATCTGGACCAGTTTGGGAAGACCAAAGTTCATCTCCAGGGTTGCATGTTTTATGGGCAGCCTGAGCCTCCTGCTCCTTGCACATGTCCACTTAGGCAAGCCTGGGGCAGCCTGGATGCTCTCATAGGGAGGCTGAGGGCTGCCTACGAAGAAAATGGAGGATCGCCGGAGAACAACCCTTTCGCTAGTGGTGCAATCAGGGTTTACCTGAGGGAGGTCAGGGAGTGCCAAGCTATGGCAAGAGGGATTCCTTTTAAGAAGAAAAAGAAGAAGCCAAGTCCAAGCAAGGGATCCGGACCAAATGATGATGAATCAAGTTCTACCATGCATTTTTCTTGA

Protein Analysis

182

Amino Acids

20.18

Weight (kDa)

9.21

Isoelectric Point (pI)

64.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 22 - 144 2.6e-66 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 193, 442
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 2 cut(s) 42, 91
AclWI GGATC 4 cut(s) 43, 376, 492, 505
AcoI YGGCCR 1 cut(s) 78
AfaI GTAC 2 cut(s) 137, 192
AfiI CCNNNNNNNGG 4 cut(s) 193, 421, 442, 494
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 1 cut(s) 142
AjnI CCWGG 3 cut(s) 243, 308, 318
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 4 cut(s) 43, 376, 492, 505
Aor13HI TCCGGA 1 cut(s) 500
AoxI GGCC 2 cut(s) 78, 152
ApeKI GCWGC 5 cut(s) 70, 73, 265, 315, 347
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 2 cut(s) 214, 503
AvaII GGWCC 2 cut(s) 214, 503
AxyI CCTNAGG 1 cut(s) 416
BamHI GGATCC 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 233
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 5 cut(s) 82, 85, 277, 327, 334
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 3 cut(s) 245, 310, 320
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 167
BisI GCNGC 6 cut(s) 71, 74, 91, 266, 316, 348
BlsI GCNGC 6 cut(s) 72, 75, 92, 267, 317, 349
BmcAI AGTACT 1 cut(s) 137
Bme1390I CCNGG 3 cut(s) 245, 310, 320
Bme18I GGWCC 2 cut(s) 214, 503
BmgT120I GGNCC 2 cut(s) 214, 503
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 3 cut(s) 245, 310, 320
BmsI GCATC 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 233
BpmI CTGGAG 5 cut(s) 27, 66, 141, 215, 227
Bpu10I CCTNAGC 2 cut(s) 270, 341
BsaJI CCNNGG 2 cut(s) 244, 309
BsaWI WCCGGW 1 cut(s) 500
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 421, 442, 494
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 4 cut(s) 122, 158, 198, 217
Bse21I CCTNAGG 1 cut(s) 416
BseAI TCCGGA 1 cut(s) 500
BseBI CCWGG 3 cut(s) 245, 310, 320
BseDI CCNNGG 2 cut(s) 244, 309
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 4 cut(s) 193, 421, 442, 494
BseMII CTCAG 4 cut(s) 185, 261, 332, 407
BseNI ACTGG 4 cut(s) 122, 158, 198, 217
BseXI GCAGC 5 cut(s) 82, 85, 277, 327, 334
BshFI GGCC 2 cut(s) 80, 154
BsiSI CCGG 3 cut(s) 77, 374, 501
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 4 cut(s) 193, 421, 442, 494
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 2 cut(s) 80, 154
Bsp13I TCCGGA 1 cut(s) 500
Bsp143I GATC 4 cut(s) 35, 64, 368, 497
BspACI CCGC 2 cut(s) 42, 91
BspANI GGCC 2 cut(s) 80, 154
BspCNI CTCAG 4 cut(s) 184, 262, 333, 408
BspEI TCCGGA 1 cut(s) 500
BspLI GGNNCC 1 cut(s) 499
BspPI GGATC 4 cut(s) 43, 376, 492, 505
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 4 cut(s) 122, 158, 198, 217
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 244, 309
BssMI GATC 4 cut(s) 35, 64, 368, 497
Bst2UI CCWGG 3 cut(s) 245, 310, 320
BstC8I GCNNGC 2 cut(s) 78, 306
BstDEI CTNAG 6 cut(s) 171, 203, 270, 299, 341, 416
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 4 cut(s) 38, 67, 371, 500
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 4 cut(s) 35, 64, 368, 497
BstMWI GCNNNNNNNGC 2 cut(s) 271, 398
BstNI CCWGG 3 cut(s) 245, 310, 320
BstNSI RCATGY 2 cut(s) 256, 294
BstSCI CCNGG 3 cut(s) 243, 308, 318
BstV1I GCAGC 5 cut(s) 82, 85, 277, 327, 334
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
Bsu36I CCTNAGG 1 cut(s) 416
BsuRI GGCC 2 cut(s) 80, 154
BtsCI GGATG 1 cut(s) 328
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 78, 306
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 214, 503
Csp6I GTAC 2 cut(s) 136, 191
CviAII CATG 4 cut(s) 188, 253, 291, 532
CviQI GTAC 2 cut(s) 136, 191
DdeI CTNAG 6 cut(s) 171, 203, 270, 299, 341, 416
DpnI GATC 4 cut(s) 37, 66, 370, 499
DpnII GATC 4 cut(s) 35, 64, 368, 497
EaeI YGGCCR 1 cut(s) 78
Eco147I AGGCCT 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 214, 503
Eco81I CCTNAGG 1 cut(s) 416
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoRII CCWGG 3 cut(s) 243, 308, 318
EcoT22I ATGCAT 1 cut(s) 537
FaeI CATG 4 cut(s) 191, 256, 294, 535
FaiI YATR 8 cut(s) 96, 189, 254, 261, 292, 332, 443, 533
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 187, 252, 290, 531
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 6 cut(s) 71, 74, 91, 266, 316, 348
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 6 cut(s) 71, 74, 91, 266, 316, 348
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 165
GluI GCNGC 6 cut(s) 71, 74, 91, 266, 316, 348
GsuI CTGGAG 5 cut(s) 27, 66, 141, 215, 227
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 2 cut(s) 80, 154
HapII CCGG 3 cut(s) 77, 374, 501
HhaI GCGC 1 cut(s) 166
Hin1II CATG 4 cut(s) 191, 256, 294, 535
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 3 cut(s) 98, 454, 518
HpaII CCGG 3 cut(s) 77, 374, 501
Hpy166II GTNNAC 2 cut(s) 296, 412
Hpy188I TCNGA 2 cut(s) 57, 174
Hpy188III TCNNGA 4 cut(s) 6, 212, 501, 543
Hpy8I GTNNAC 2 cut(s) 296, 412
HpyAV CCTTC 2 cut(s) 26, 105
HpyCH4V TGCA 4 cut(s) 252, 288, 401, 535
HpyF10VI GCNNNNNNNGC 2 cut(s) 271, 398
HpyF3I CTNAG 6 cut(s) 171, 203, 270, 299, 341, 416
Hsp92II CATG 4 cut(s) 191, 256, 294, 535
HspAI GCGC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 500
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 4 cut(s) 35, 64, 368, 497
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 5 cut(s) 82, 85, 277, 327, 334
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 393
MalI GATC 4 cut(s) 37, 66, 370, 499
MboI GATC 4 cut(s) 35, 64, 368, 497
MboII GAAGA 5 cut(s) 154, 238, 368, 478, 487
MflI RGATCY 1 cut(s) 497
MluCI AATT 1 cut(s) 178
Mph1103I ATGCAT 1 cut(s) 537
MroI TCCGGA 1 cut(s) 500
MroNI GCCGGC 1 cut(s) 76
MseI TTAA 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 3 cut(s) 77, 374, 501
MspR9I CCNGG 3 cut(s) 245, 310, 320
MvaI CCWGG 3 cut(s) 245, 310, 320
MwoI GCNNNNNNNGC 2 cut(s) 271, 398
NaeI GCCGGC 1 cut(s) 78
NdeII GATC 4 cut(s) 35, 64, 368, 497
NgoMIV GCCGGC 1 cut(s) 76
NlaIII CATG 4 cut(s) 191, 256, 294, 535
NlaIV GGNNCC 1 cut(s) 499
NsiI ATGCAT 1 cut(s) 537
NspI RCATGY 2 cut(s) 256, 294
PceI AGGCCT 1 cut(s) 154
PciI ACATGT 1 cut(s) 290
PdiI GCCGGC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 98, 454, 518
PflMI CCANNNNNTGG 2 cut(s) 193, 442
PkrI GCNGC 6 cut(s) 72, 75, 92, 267, 317, 349
PscI ACATGT 1 cut(s) 290
Psp6I CCWGG 3 cut(s) 243, 308, 318
PspGI CCWGG 3 cut(s) 243, 308, 318
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 2 cut(s) 214, 503
PsuI RGATCY 1 cut(s) 497
RsaI GTAC 2 cut(s) 137, 192
RsaNI GTAC 2 cut(s) 136, 191
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 6 cut(s) 71, 74, 91, 266, 316, 348
Sau3AI GATC 4 cut(s) 35, 64, 368, 497
Sau96I GGNCC 2 cut(s) 214, 503
ScaI AGTACT 1 cut(s) 137
ScrFI CCNGG 3 cut(s) 245, 310, 320
SetI ASST 5 cut(s) 52, 209, 417, 426, 442
SfaNI GCATC 1 cut(s) 313
SinI GGWCC 2 cut(s) 214, 503
Sse9I AATT 1 cut(s) 178
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 2 cut(s) 42, 91
SspMI CTAG 1 cut(s) 393
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 3 cut(s) 243, 308, 318
TasI AATT 1 cut(s) 178
TatI WGTACW 2 cut(s) 135, 190
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 3 cut(s) 98, 454, 518
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 1 cut(s) 165
TseI GCWGC 5 cut(s) 70, 73, 265, 315, 347
TspDTI ATGAA 3 cut(s) 111, 227, 531
TspRI CASTG 1 cut(s) 165
Van91I CCANNNNNTGG 2 cut(s) 193, 442
VpaK11BI GGWCC 2 cut(s) 214, 503
XagI CCTNNNNNAGG 1 cut(s) 419
XceI RCATGY 2 cut(s) 256, 294
XspI CTAG 1 cut(s) 393
ZrmI AGTACT 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.