pycom10g01120

DUF218 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011939
Physical Location & Seq
Reverse (-)
1207768 .. 1210860
3093 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1047 bp
ATGATGAGCAATAATCCGTTTCGGCCCGGCAGTCCTAAGTCCTTCGTAGCCTACCCGAGTGGTGAGTTTGATTTAGAATCTGGAACATTTAGAAAACCCCGAAAGCATAGGAGGTCACCATCGCTTCCCATCAAAATGATTAGGTATATCGGAAACCGAATGCAGTATTATTGCAAGCTGCGTCCCCTTTTAGTTTTCCTCCTCTCCTTGTCAGTTGGGGTCTCAATTCTCATACTTTTGTCTCTGTATGAGAGCCGGTATCAAATGACGAACAACTACCAAAAGTATGACGTGGGTTCTTCTAGTTATCCGCTCCCGAATCTCCAGAATCTAGTCATGGTTGCCGGGCATTCAGTTTATACTAGTAGTAGCTGTGGAAAAGTTGATAAGGAGGATTCTTGGTTTTTGGAGTCTTACCAAAAGAATCCGGGTCAGGCTGCTACTTTTGTGGCTCATATACAAGAGGGAATTGAAGTCGCAGCCAAAGACAATAGAGCTCTCCTTCTGTTTAGTGGTGGAGAGACTCGAAGAGATGCCGGTCCTCGCAGTGAGGCACAGAGTTACTGGGCAGTTGCTGAATCAAAAGGATGGTTTGGCCAAGAAGATTTGCGATCGAGGACACTCACAGAAGAGCATGCAAGGGACAGCTTTGAGAATCTTCTCTTCAGTGTGTGCCGGTTCCGAGAACTTACTGGCACATATCCTCAAAATATAACTGTTGTAAGTTATGATTTCAAGGAGGAGAGATTCGCGCATCTACACCGGGCTGCAATTGGCTTTCCTGAGTCAAGGTTCTTCTACTTGGGCACCCCGGCAATTTCTACTGCAAAAGAAGCAGCCCGGAAAGGAGAAGCAGTCGTGCGGACTCAATTCCAAGAAGATCCATATGGATGTCGAGGTTCACTTTACCGTAAAAAACTAGGGCGCGATCCGTTTCACCGGTCCGTTCCCTATCCAAATGGGTGTCCTGAAATTGAAGGTCTTTTCAGATATTGTGGGTCAGCTCCTTATCCAGGGTCCCTTCCGTGGCCTAAAGAGCAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF218

Protein Analysis

349

Amino Acids

39.57

Weight (kDa)

9.22

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21370 AT1G21370
fragaria_vesca FvH4_2g14460 FvH4_2g14460
malus_domestica MD05G1017500.v1.1 MD10G1017500.v1.1
prunus_persica Prupe.8G021400_v2.0.a1
pyrus_communis pycom05g00980 pycom10g01120
rosa_chinensis RchiOBHm_Chr6g0276791
rosa_laevigata RLG00000013361
rosa_multiflora Rmu_co8479231.1_g000001
rosa_roxburghii Rroxscaffold_7G00191940
rosa_rugosa Rorug06G0104500
rosa_samantha Rh6AG214400 Rh6BG219200 Rh6CG221900 Rh6DG211900
rosa_wichuraiana Rw6G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 806
AccBSI CCGCTC 1 cut(s) 313
AccII CGCG 2 cut(s) 752, 927
AciI CCGC 2 cut(s) 311, 862
AclWI GGATC 2 cut(s) 875, 923
AcoI YGGCCR 1 cut(s) 595
AcuI CTGAAG 1 cut(s) 649
AfiI CCNNNNNNNGG 2 cut(s) 1013, 1026
AgeI ACCGGT 1 cut(s) 939
AgsI TTSAA 3 cut(s) 473, 736, 977
AhlI ACTAGT 1 cut(s) 362
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 1012
AluBI AGCT 5 cut(s) 178, 372, 497, 648, 1004
AluI AGCT 5 cut(s) 178, 372, 497, 648, 1004
Alw21I GWGCWC 1 cut(s) 499
Alw26I GTCTC 3 cut(s) 226, 246, 515
AlwI GGATC 2 cut(s) 875, 923
AlwNI CAGNNNCTG 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 55
AoxI GGCC 3 cut(s) 23, 595, 1028
ApeKI GCWGC 5 cut(s) 178, 437, 479, 767, 836
AsiGI ACCGGT 1 cut(s) 939
AspLEI GCGC 2 cut(s) 754, 927
AspS9I GGNCC 4 cut(s) 24, 539, 942, 1017
AsuC2I CCSGG 6 cut(s) 27, 346, 429, 764, 812, 841
AsuHPI GGTGA 3 cut(s) 74, 108, 929
AvaI CYCGRG 1 cut(s) 55
AvaII GGWCC 3 cut(s) 539, 942, 1017
BaeGI GKGCMC 1 cut(s) 809
BalI TGGCCA 1 cut(s) 597
BanI GGYRCC 1 cut(s) 806
BanII GRGCYC 1 cut(s) 499
Bbv12I GWGCWC 1 cut(s) 499
BbvI GCAGC 5 cut(s) 165, 424, 491, 754, 848
BccI CCATC 3 cut(s) 127, 137, 582
BciT130I CCWGG 1 cut(s) 1014
BcnI CCSGG 6 cut(s) 27, 346, 429, 764, 812, 841
BcoDI GTCTC 3 cut(s) 226, 246, 515
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 4 cut(s) 303, 332, 363, 920
BisI GCNGC 5 cut(s) 179, 438, 480, 768, 837
BlsI GCNGC 5 cut(s) 180, 439, 481, 769, 838
Bme1390I CCNGG 7 cut(s) 27, 346, 429, 764, 812, 841, 1014
Bme18I GGWCC 3 cut(s) 539, 942, 1017
BmeT110I CYCGRG 1 cut(s) 55
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 4 cut(s) 24, 539, 942, 1017
BmiI GGNNCC 4 cut(s) 680, 808, 1018, 1019
BmrFI CCNGG 7 cut(s) 27, 346, 429, 764, 812, 841, 1014
BmrI ACTGGG 1 cut(s) 574
BmsI GCATC 2 cut(s) 523, 763
BmuI ACTGGG 1 cut(s) 574
BpmI CTGGAG 1 cut(s) 308
BpuMI CCSGG 6 cut(s) 27, 346, 429, 764, 812, 841
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 3 cut(s) 810, 1013, 1025
BsaWI WCCGGW 1 cut(s) 939
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 2 cut(s) 1013, 1026
Bse118I RCCGGY 4 cut(s) 255, 536, 675, 939
Bse1I ACTGG 2 cut(s) 569, 697
BseBI CCWGG 1 cut(s) 1014
BseDI CCNNGG 3 cut(s) 810, 1013, 1025
BseGI GGATG 2 cut(s) 593, 896
BseLI CCNNNNNNNGG 2 cut(s) 1013, 1026
BseMII CTCAG 1 cut(s) 774
BseNI ACTGG 2 cut(s) 569, 697
BseRI GAGGAG 2 cut(s) 191, 755
BseSI GKGCMC 1 cut(s) 809
BseXI GCAGC 5 cut(s) 165, 424, 491, 754, 848
Bsh1236I CGCG 2 cut(s) 752, 927
Bsh1285I CGRYCG 1 cut(s) 614
BshFI GGCC 3 cut(s) 25, 597, 1030
BshNI GGYRCC 1 cut(s) 806
BshTI ACCGGT 1 cut(s) 939
BsiEI CGRYCG 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 499
BsiHKCI CYCGRG 1 cut(s) 55
BslFI GGGAC 3 cut(s) 168, 656, 1003
BslI CCNNNNNNNGG 2 cut(s) 1013, 1026
BsmAI GTCTC 3 cut(s) 226, 246, 515
BsmFI GGGAC 3 cut(s) 168, 656, 1003
BsmI GAATGC 2 cut(s) 165, 349
BsnI GGCC 3 cut(s) 25, 597, 1030
Bso31I GGTCTC 1 cut(s) 226
BsoBI CYCGRG 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 499, 809
Bsp143I GATC 3 cut(s) 611, 880, 928
BspACI CCGC 2 cut(s) 311, 862
BspANI GGCC 3 cut(s) 25, 597, 1030
BspCNI CTCAG 1 cut(s) 775
BspFNI CGCG 2 cut(s) 752, 927
BspLI GGNNCC 4 cut(s) 680, 808, 1018, 1019
BspPI GGATC 2 cut(s) 875, 923
BspQI GCTCTTC 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 806
BspTNI GGTCTC 1 cut(s) 226
BsrBI CCGCTC 1 cut(s) 313
BsrFI RCCGGY 4 cut(s) 255, 536, 675, 939
BsrI ACTGG 2 cut(s) 569, 697
BssAI RCCGGY 4 cut(s) 255, 536, 675, 939
BssECI CCNNGG 3 cut(s) 810, 1013, 1025
BssMI GATC 3 cut(s) 611, 880, 928
Bst2UI CCWGG 1 cut(s) 1014
Bst4CI ACNGT 2 cut(s) 718, 911
Bst6I CTCTTC 3 cut(s) 523, 624, 668
BstC8I GCNNGC 2 cut(s) 176, 636
BstDEI CTNAG 2 cut(s) 36, 783
BstDSI CCRYGG 1 cut(s) 1025
BstEII GGTNACC 1 cut(s) 114
BstENI CCTNNNNNAGG 1 cut(s) 1011
BstF5I GGATG 2 cut(s) 593, 896
BstFNI CGCG 2 cut(s) 752, 927
BstHHI GCGC 2 cut(s) 754, 927
BstKTI GATC 3 cut(s) 614, 883, 931
BstMAI GTCTC 3 cut(s) 226, 246, 515
BstMBI GATC 3 cut(s) 611, 880, 928
BstMCI CGRYCG 1 cut(s) 614
BstMWI GCNNNNNNNGC 2 cut(s) 833, 1036
BstNI CCWGG 1 cut(s) 1014
BstNSI RCATGY 1 cut(s) 638
BstPI GGTNACC 1 cut(s) 114
BstSCI CCNGG 7 cut(s) 25, 344, 427, 762, 810, 839, 1012
BstSLI GKGCMC 1 cut(s) 809
BstUI CGCG 2 cut(s) 752, 927
BstV1I GCAGC 5 cut(s) 165, 424, 491, 754, 848
BstX2I RGATCY 1 cut(s) 880
BstYI RGATCY 1 cut(s) 880
BsuRI GGCC 3 cut(s) 25, 597, 1030
BtgI CCRYGG 1 cut(s) 1025
BtgZI GCGATG 1 cut(s) 105
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 2 cut(s) 593, 896
BtsI GCAGTG 1 cut(s) 553
BtsIMutI CAGTG 2 cut(s) 553, 673
Cac8I GCNNGC 2 cut(s) 176, 636
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 2 cut(s) 754, 927
Cfr10I RCCGGY 4 cut(s) 255, 536, 675, 939
Cfr13I GGNCC 4 cut(s) 24, 539, 942, 1017
CpoI CGGWCCG 1 cut(s) 942
CseI GACGC 1 cut(s) 170
CspAI ACCGGT 1 cut(s) 939
CspI CGGWCCG 1 cut(s) 942
CviAII CATG 2 cut(s) 337, 635
DdeI CTNAG 2 cut(s) 36, 783
DpnI GATC 3 cut(s) 613, 882, 930
DpnII GATC 3 cut(s) 611, 880, 928
EaeI YGGCCR 1 cut(s) 595
Eam1104I CTCTTC 3 cut(s) 523, 624, 668
EarI CTCTTC 3 cut(s) 523, 624, 668
Ecl136II GAGCTC 1 cut(s) 497
Eco24I GRGCYC 1 cut(s) 499
Eco31I GGTCTC 1 cut(s) 226
Eco47I GGWCC 3 cut(s) 539, 942, 1017
Eco53kI GAGCTC 1 cut(s) 497
Eco57I CTGAAG 1 cut(s) 649
Eco88I CYCGRG 1 cut(s) 55
Eco91I GGTNACC 1 cut(s) 114
EcoICRI GAGCTC 1 cut(s) 497
EcoNI CCTNNNNNAGG 1 cut(s) 1011
EcoO109I RGGNCCY 1 cut(s) 1017
EcoO65I GGTNACC 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 1012
EcoT38I GRGCYC 1 cut(s) 499
FaeI CATG 2 cut(s) 340, 638
FaqI GGGAC 3 cut(s) 168, 656, 1003
FatI CATG 2 cut(s) 336, 634
FauNDI CATATG 1 cut(s) 886
Fnu4HI GCNGC 5 cut(s) 179, 438, 480, 768, 837
FokI GGATG 2 cut(s) 600, 903
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 5 cut(s) 179, 438, 480, 768, 837
FspBI CTAG 4 cut(s) 303, 332, 363, 920
GlaI GCGC 2 cut(s) 753, 926
GluI GCNGC 5 cut(s) 179, 438, 480, 768, 837
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 3 cut(s) 25, 597, 1030
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 754, 927
Hin1II CATG 2 cut(s) 340, 638
Hin6I GCGC 2 cut(s) 752, 925
HinP1I GCGC 2 cut(s) 752, 925
HphI GGTGA 3 cut(s) 74, 108, 929
Hpy166II GTNNAC 1 cut(s) 902
Hpy188I TCNGA 3 cut(s) 152, 683, 989
Hpy188III TCNNGA 5 cut(s) 81, 316, 325, 782, 968
Hpy8I GTNNAC 1 cut(s) 902
HpyAV CCTTC 4 cut(s) 52, 512, 971, 1031
HpyCH4III ACNGT 2 cut(s) 718, 911
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 5 cut(s) 163, 174, 638, 770, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 833, 1036
HpyF3I CTNAG 2 cut(s) 36, 783
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 2 cut(s) 340, 638
HspAI GCGC 2 cut(s) 752, 925
KflI GGGWCCC 1 cut(s) 1017
Kzo9I GATC 3 cut(s) 611, 880, 928
LguI GCTCTTC 1 cut(s) 624
LmnI GCTCC 2 cut(s) 318, 1009
Lsp1109I GCAGC 5 cut(s) 165, 424, 491, 754, 848
LweI GCATC 2 cut(s) 523, 763
MaeI CTAG 4 cut(s) 303, 332, 363, 920
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 114, 560
MalI GATC 3 cut(s) 613, 882, 930
MbiI CCGCTC 1 cut(s) 313
MboI GATC 3 cut(s) 611, 880, 928
MboII GAAGA 8 cut(s) 291, 540, 614, 641, 650, 655, 787, 890
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 1 cut(s) 880
MhlI GDGCHC 2 cut(s) 499, 809
MlsI TGGCCA 1 cut(s) 597
MluCI AATT 7 cut(s) 225, 468, 771, 816, 869, 972, 1042
MluNI TGGCCA 1 cut(s) 597
MlyI GAGTC 4 cut(s) 419, 517, 794, 859
Mox20I TGGCCA 1 cut(s) 597
MscI TGGCCA 1 cut(s) 597
MseI TTAA 1 cut(s) 1045
MslI CAYNNNNRTG 2 cut(s) 134, 889
Msp20I TGGCCA 1 cut(s) 597
MspR9I CCNGG 7 cut(s) 27, 346, 429, 764, 812, 841, 1014
MunI CAATTG 1 cut(s) 771
Mva1269I GAATGC 2 cut(s) 165, 349
MvaI CCWGG 1 cut(s) 1014
MvnI CGCG 2 cut(s) 752, 927
MwoI GCNNNNNNNGC 2 cut(s) 833, 1036
NciI CCSGG 6 cut(s) 27, 346, 429, 764, 812, 841
NdeI CATATG 1 cut(s) 886
NdeII GATC 3 cut(s) 611, 880, 928
NlaIII CATG 2 cut(s) 340, 638
NlaIV GGNNCC 4 cut(s) 680, 808, 1018, 1019
NmuCI GTSAC 1 cut(s) 114
NspI RCATGY 1 cut(s) 638
PaeI GCATGC 1 cut(s) 638
PciSI GCTCTTC 1 cut(s) 624
PctI GAATGC 2 cut(s) 165, 349
PfeI GAWTC 8 cut(s) 77, 319, 328, 395, 424, 578, 655, 747
PinAI ACCGGT 1 cut(s) 939
PkrI GCNGC 5 cut(s) 180, 439, 481, 769, 838
Ple19I CGATCG 1 cut(s) 614
PleI GAGTC 4 cut(s) 418, 517, 793, 859
PpsI GAGTC 4 cut(s) 418, 517, 793, 859
PpuMI RGGWCCY 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 499
Psp5II RGGWCCY 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 1012
PspEI GGTNACC 1 cut(s) 114
PspGI CCWGG 1 cut(s) 1012
PspN4I GGNNCC 4 cut(s) 680, 808, 1018, 1019
PspPI GGNCC 4 cut(s) 24, 539, 942, 1017
PspPPI RGGWCCY 1 cut(s) 1017
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 1 cut(s) 880
PvuI CGATCG 1 cut(s) 614
RseI CAYNNNNRTG 2 cut(s) 134, 889
Rsr2I CGGWCCG 1 cut(s) 942
RsrII CGGWCCG 1 cut(s) 942
SacI GAGCTC 1 cut(s) 499
SapI GCTCTTC 1 cut(s) 624
SaqAI TTAA 1 cut(s) 1045
SatI GCNGC 5 cut(s) 179, 438, 480, 768, 837
Sau3AI GATC 3 cut(s) 611, 880, 928
Sau96I GGNCC 4 cut(s) 24, 539, 942, 1017
SchI GAGTC 4 cut(s) 419, 517, 794, 859
ScrFI CCNGG 7 cut(s) 27, 346, 429, 764, 812, 841, 1014
SduI GDGCHC 2 cut(s) 499, 809
SfaNI GCATC 2 cut(s) 523, 763
SinI GGWCC 3 cut(s) 539, 942, 1017
SmiMI CAYNNNNRTG 2 cut(s) 134, 889
SpeI ACTAGT 1 cut(s) 362
SphI GCATGC 1 cut(s) 638
Sse9I AATT 7 cut(s) 225, 468, 771, 816, 869, 972, 1042
SsiI CCGC 2 cut(s) 311, 862
SspMI CTAG 4 cut(s) 303, 332, 363, 920
SstI GAGCTC 1 cut(s) 499
StyD4I CCNGG 7 cut(s) 25, 344, 427, 762, 810, 839, 1012
TaaI ACNGT 2 cut(s) 718, 911
TaiI ACGT 1 cut(s) 294
TaqI TCGA 3 cut(s) 526, 614, 895
TasI AATT 7 cut(s) 225, 468, 771, 816, 869, 972, 1042
TfiI GAWTC 8 cut(s) 77, 319, 328, 395, 424, 578, 655, 747
Tru1I TTAA 1 cut(s) 1045
Tru9I TTAA 1 cut(s) 1045
TscAI CASTG 2 cut(s) 553, 673
TseFI GTSAC 1 cut(s) 114
TseI GCWGC 5 cut(s) 178, 437, 479, 767, 836
Tsp45I GTSAC 1 cut(s) 114
TspGWI ACGGA 4 cut(s) 6, 921, 934, 1014
TspRI CASTG 2 cut(s) 553, 673
VpaK11BI GGWCC 3 cut(s) 539, 942, 1017
XagI CCTNNNNNAGG 1 cut(s) 1011
XceI RCATGY 1 cut(s) 638
XspI CTAG 4 cut(s) 303, 332, 363, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.