Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0013282

Physical Location & Seq

Genomic Coordinates Forward (+)

6282316 .. 6287954

Gene Structure 5639 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGGGAAAGGTGAAGGGCAAGCACCGTTTGGACAAGTACTACAAACTAGCCAAGGAGCATGGCTACCGCTCCCGAGCTTCATGGAAGCTTGCCCAGCTCGACACCAAGCACCAATTCCTTCACTCTTCTCACGCCGTCCTCGACCTCTGCGCCGCGCCCGGCGGTTGGATGCAAGTCGCCGTGCAGCGCGTTCCCGTCGGCAGCCTCGTGGTCGGCGTCGACTTGGTCCCTATCGCCCCTGTCCGCGGCGCCTTCTCGATCCAACAGGACATCACCAAGCCAGAATGCACGGCCAGGCTCAGAAAGCTGATGAAGGAGAATGGCTGTTCGGCTTTTGATTTGGTGCTTCATGATGGGTCGCCCAATGTCGGCGGCGCGTGGTCTTCGGAGGCCACTGCTCAGAATGCTTTGGTCGTTGATGCGGTGAAGTTGGCTACTCAGCTTTTGGCTCCCAAAGGAACATTTATTACTAAAATCTTCAGGTCGCAAGATTATAATGCTGTCTACTATTGTATGAAGGAGTTGTTTGAGAAGGTTGAACAGGATAAACCAGCAGCTAGTCGTAGCTCTTCTGCGGAGACGTATCTTTTGGGTTTTAAGTACAAGGCTCCTGCAAAGATTGATCCGCGTCTACTTGACGTGAAACATCTATTTAAGTCTGTAGAACCTCAGAAGAAGGTGGTAGATGTACTAAGAGGAACAAAGCAAAAAAGACACCGTGATGGGTATGAAGATGGAGACACAATTTTGAGAAAAGTGTCTTCTGCTGCTGCTTTCATTTGGTTAAAACTCTCAGTGATCGATCTGCGTGTGTTGGGGAAGCAAGATTTCAAGTACCTTTTGAAGTGGCGGATGCAGATAAAAAAGGCCTTATCTCCGTCCCAGAAGGCTGACGCTTCTACTACAGATGTTGAAAAGGAGGAAAATGAGGAGGATGATGAAGACAAAATACTCAATGAAATGGAGGAGCTGACGTATGCTATGGAGCGCAAGAAGAAACGAACAAAGAAGCTTCTAGCAAAAAGACGAGCTCAAGACAAGGTACGGAAGGCAACAGGGATGCAAATAGATGCACTACAAGATGGTTATACTGACAATGAGTTGTTTTCTCTCGCTTCCATCAAGGGTAAGAACGATCTGGTAGTGGTTGATTCCACTGAATATGATGGTGAGAATGGTGACCTTGGAGATACTGAAAATGGGGAAACCCATGAGAATACCCAAGAAGCATCATCCAGTGATATGGACTCTGATGATGAACGCAGAAGATATGATGCACAAATGGAAGATCTTCTCGATAAGGCATATGAACAGTACACCTCCAAAAAGGAGGGAAGTGCAAAGCAGCGGAAACGTTTAAAACAAGCACATTCTGAGGACGCTCAACTTTTGGAGGATGTTGATGGCGGTGATATAGTTCAGTCTGATTATGATTCAGACAAAGACCAGGGTATTCAGGAATCGAATCCTCTCTTGGAATCACTTGATGAGGGGGACGGACCTACCCAAGAGGAAATTATGAATAACTGGTTTAGTCAGGATATCTTTGCCGAAGCTGTAGAGCAGGGGGATTTGGATAACTCTGGTAGTGAAGATGAAATGCAGGTTGAAAAGCAGGAGAAACTTCCCGTGCTTGAAAAGGCCAATGAAAAGACTGCAATTCAGAATGGTAAGGAAAAGACTGCAATTCAGAATGCTAAGAAAAATACGGAAAATGATGCTGCAGGGACCAAAGGCCATAAACTTCAAGCTTCAAAGGCCGACGATGATTTTGAGATTGTCCGTGCTCCGGGTACAGATTCAAGTGATGACTCATCATCAGATGAATCCGAGGATATGGATATAGATAAAAAAGCCGAGATACTGGCGTGTGCAAAGAAAATGCTGAGGAAAAAGCCAAGGGAACAGATGCTTGATGATGCCTATAACAAGTACATGTTTGATGATGACGGCTTGCCCAGATGGTTTTTAGATGAGGAGAAGAGGCACCGTCAGCCAATGAAACCTGTGACCAAAGAAGAGATTAATGCCATGAAAGCACAATTTAAAGAAATTGATGCCCGTCCTGCAAAGAAGGTGGCAGAGGCTAAAGCTCGAAAGAAGCGAGTTGCCTTTAGGAAGCTCGAGAAGGTTCGGAAGAAGGCAAACACAATATCTGACCAGCCAGACATCTCTGATCGTTCAAAGAGGAAGCAAATCGAACAAATGTATAAGAAGGCTGCGCCTAAAAGGCCTCAAAAGGAGTATGTGGTTGCAAAGAAGGGAGTTCAAGTCAGGGTTGGCAAGGGTAAAGTCCGTGTGGATAGACGGATGAAAAAGGATGCAAGGGGTAGTGGGCCGGGTAAAAGGCCTGGAAAAGGCAATTCGAAGAAAGGAAAAACTGCTGCCAAGGGTGAGAGAGGAGGCAAGGGTACCGGAAAGGGTGACAGAGGCAAGGGCCCCGGAAAGGCTTCAGGAAAGCCAACAGGCAGGAAGGGTAGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000460 GO:0000463 GO:0000466 GO:0000470 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008649 GO:0008650 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016435 GO:0016436 GO:0016740 GO:0016741 GO:0022613 GO:0030684 GO:0030687 GO:0031167 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3381 FtsJ Spb1_C

Protein Analysis

829

Amino Acids

92.86

Weight (kDa)

9.14

Isoelectric Point (pI)

38.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsJ	PF01728	22 - 201	1e-47	FtsJ-like methyltransferase
DUF3381	PF11861	266 - 349	2.6e-15	Ribosomal RNA methyltransferase Spb1, DUF3381
Spb1_C	PF07780	585 - 778	1.2e-59	Spb1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013282)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25730
fragaria_vesca	FvH4_2g09160
malus_domestica	MD05G1064300.v1.1 MD10G1073000.v1.1
prunus_persica	Prupe.8G094400_v2.0.a1
pyrus_communis	pycom05g05540 pycom10g05760
rosa_chinensis	RchiOBHm_Chr6g0265021
rosa_laevigata	RLG00000014215 RLG00000014220
rosa_multiflora	Rmu_sc0002934.1_g000022
rosa_roxburghii	Rroxscaffold_175G00432100
rosa_rugosa	Rorug06G0013800
rosa_samantha	Rh6AG136200 Rh6BG133000 Rh6CG131600 Rh6DG118800
rosa_wichuraiana	Rw6G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	495
Acc36I	ACCTGC	1 cut(s)	1594
Acc65I	GGTACC	1 cut(s)	2411
AccB1I	GGYRCC	3 cut(s)	248, 1986, 2411
AccBSI	CCGCTC	1 cut(s)	69
AccI	GTMKAC	3 cut(s)	219, 504, 631
AccII	CGCG	5 cut(s)	155, 189, 246, 377, 628
AclI	AACGTT	1 cut(s)	1354
AclWI	GGATC	2 cut(s)	253, 617
AcoI	YGGCCR	1 cut(s)	291
AcuI	CTGAAG	2 cut(s)	463, 2436
AcyI	GRCGYC	2 cut(s)	216, 249
AfaI	GTAC	9 cut(s)	38, 602, 690, 836, 1044, 1316, 1795, 1934, 2413
AfiI	CCNNNNNNNGG	6 cut(s)	165, 245, 368, 1789, 2357, 2446
AflIII	ACRYGT	1 cut(s)	1935
AjiI	CACGTC	1 cut(s)	640
AjnI	CCWGG	3 cut(s)	293, 1446, 2350
AjuI	GAANNNNNNNTTGG	4 cut(s)	98, 130, 1457, 1489
Alw21I	GWGCWC	2 cut(s)	1033, 1789
Alw26I	GTCTC	2 cut(s)	572, 732
AlwI	GGATC	2 cut(s)	253, 617
Ama87I	CYCGRG	2 cut(s)	72, 2123
ApaI	GGGCCC	1 cut(s)	2441
ApeKI	GCWGC	9 cut(s)	184, 201, 554, 767, 770, 1345, 1721, 2219, 2384
ArsI	GACNNNNNNTTYG	4 cut(s)	571, 603, 1754, 1786
AseI	ATTAAT	1 cut(s)	2025
Asp700I	GAANNNNTTC	2 cut(s)	1290, 2449
Asp718I	GGTACC	1 cut(s)	2411
AspLEI	GCGC	7 cut(s)	152, 157, 189, 251, 377, 990, 2224
AspS9I	GGNCC	6 cut(s)	226, 1499, 1728, 2336, 2437, 2438
AsuC2I	CCSGG	4 cut(s)	159, 1791, 2340, 2442
AsuHPI	GGTGA	8 cut(s)	22, 265, 436, 1181, 1190, 1421, 2405, 2435
AsuII	TTCGAA	1 cut(s)	2366
AvaI	CYCGRG	2 cut(s)	72, 2123
AvaII	GGWCC	3 cut(s)	226, 1499, 1728
BaeGI	GKGCMC	1 cut(s)	2441
BaeI	ACNNNNGTAYC	2 cut(s)	1853, 1886
BanI	GGYRCC	3 cut(s)	248, 1986, 2411
BanII	GRGCYC	2 cut(s)	1033, 2441
BarI	GAAGNNNNNNTAC	2 cut(s)	933, 965
BauI	CACGAG	1 cut(s)	206
BbsI	GAAGAC	3 cut(s)	375, 753, 948
Bbv12I	GWGCWC	2 cut(s)	1033, 1789
BbvCI	CCTCAGC	1 cut(s)	1886
BbvI	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1357, 1708, 2206, 2371
BccI	CCATC	8 cut(s)	347, 716, 728, 1076, 1127, 1160, 1397, 1956
BceAI	ACGGC	4 cut(s)	119, 164, 306, 1966
BciT130I	CCWGG	3 cut(s)	295, 1448, 2352
BcnI	CCSGG	4 cut(s)	159, 1791, 2340, 2442
BcoDI	GTCTC	2 cut(s)	572, 732
BfaI	CTAG	3 cut(s)	47, 558, 1016
BfmI	CTRYAG	4 cut(s)	660, 903, 1557, 1722
BfoI	RGCGCY	1 cut(s)	252
BfuAI	ACCTGC	1 cut(s)	1594
BglI	GCCNNNNNGGC	1 cut(s)	2230
BglII	AGATCT	1 cut(s)	1288
BmcAI	AGTACT	1 cut(s)	38
Bme1390I	CCNGG	7 cut(s)	159, 295, 1448, 1791, 2340, 2352, 2442
Bme18I	GGWCC	3 cut(s)	226, 1499, 1728
BmeT110I	CYCGRG	2 cut(s)	72, 2123
BmgBI	CACGTC	1 cut(s)	640
BmgT120I	GGNCC	6 cut(s)	226, 1499, 1728, 2336, 2437, 2438
BmiI	GGNNCC	9 cut(s)	228, 250, 450, 609, 1729, 1988, 2413, 2439, 2440
BmrFI	CCNGG	7 cut(s)	159, 295, 1448, 1791, 2340, 2352, 2442
BpiI	GAAGAC	3 cut(s)	375, 753, 948
Bpu10I	CCTNAGC	1 cut(s)	1886
Bpu14I	TTCGAA	1 cut(s)	2366
BpuEI	CTTGAG	1 cut(s)	1017
BpuMI	CCSGG	4 cut(s)	159, 1791, 2340, 2442
Bsa29I	ATCGAT	1 cut(s)	801
BsaHI	GRCGYC	2 cut(s)	216, 249
BsaJI	CCNNGG	8 cut(s)	51, 244, 1183, 1447, 1830, 1898, 2388, 2440
BsaWI	WCCGGW	1 cut(s)	2414
BsaXI	ACNNNNNCTCC	4 cut(s)	959, 989, 2256, 2286
Bsc4I	CCNNNNNNNGG	6 cut(s)	165, 245, 368, 1789, 2357, 2446
Bse1I	ACTGG	3 cut(s)	1236, 1532, 1869
BseBI	CCWGG	3 cut(s)	295, 1448, 2352
BseCI	ATCGAT	1 cut(s)	801
BseDI	CCNNGG	8 cut(s)	51, 244, 1183, 1447, 1830, 1898, 2388, 2440
BseGI	GGATG	8 cut(s)	174, 858, 940, 1065, 1232, 1402, 2316, 2326
BseLI	CCNNNNNNNGG	6 cut(s)	165, 245, 368, 1789, 2357, 2446
BseMII	CTCAG	7 cut(s)	313, 413, 452, 683, 807, 1365, 1877
BseNI	ACTGG	3 cut(s)	1236, 1532, 1869
BseRI	GAGGAG	4 cut(s)	944, 980, 1991, 2415
BseSI	GKGCMC	1 cut(s)	2441
BseXI	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1357, 1708, 2206, 2371
BseYI	CCCAGC	1 cut(s)	93
BsgI	GTGCAG	1 cut(s)	203
Bsh1236I	CGCG	5 cut(s)	155, 189, 246, 377, 628
BshNI	GGYRCC	3 cut(s)	248, 1986, 2411
BshVI	ATCGAT	1 cut(s)	801
BsiHKAI	GWGCWC	2 cut(s)	1033, 1789
BsiHKCI	CYCGRG	2 cut(s)	72, 2123
BsiSI	CCGG	5 cut(s)	159, 1790, 2339, 2415, 2442
BslFI	GGGAC	4 cut(s)	212, 865, 1508, 1741
BslI	CCNNNNNNNGG	6 cut(s)	165, 245, 368, 1789, 2357, 2446
BsmAI	GTCTC	2 cut(s)	572, 732
BsmBI	CGTCTC	1 cut(s)	572
BsmFI	GGGAC	4 cut(s)	212, 865, 1508, 1741
BsmI	GAATGC	3 cut(s)	290, 409, 1699
BsoBI	CYCGRG	2 cut(s)	72, 2123
Bsp119I	TTCGAA	1 cut(s)	2366
Bsp120I	GGGCCC	1 cut(s)	2437
Bsp1286I	GDGCHC	3 cut(s)	1033, 1789, 2441
Bsp143I	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
BspCNI	CTCAG	7 cut(s)	312, 412, 451, 682, 806, 1366, 1878
BspDI	ATCGAT	1 cut(s)	801
BspFNI	CGCG	5 cut(s)	155, 189, 246, 377, 628
BspHI	TCATGA	1 cut(s)	349
BspLI	GGNNCC	9 cut(s)	228, 250, 450, 609, 1729, 1988, 2413, 2439, 2440
BspMAI	CTGCAG	1 cut(s)	1726
BspMI	ACCTGC	1 cut(s)	1594
BspPI	GGATC	2 cut(s)	253, 617
BspQI	GCTCTTC	1 cut(s)	574
BspT104I	TTCGAA	1 cut(s)	2366
BspT107I	GGYRCC	3 cut(s)	248, 1986, 2411
BsrBI	CCGCTC	1 cut(s)	69
BsrI	ACTGG	3 cut(s)	1236, 1532, 1869
BssECI	CCNNGG	8 cut(s)	51, 244, 1183, 1447, 1830, 1898, 2388, 2440
BssMI	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
BssNI	GRCGYC	2 cut(s)	216, 249
BssSI	CACGAG	1 cut(s)	206
BssT1I	CCWWGG	4 cut(s)	51, 1183, 1898, 2388
Bst2BI	CACGAG	1 cut(s)	206
Bst2UI	CCWGG	3 cut(s)	295, 1448, 2352
Bst4CI	ACNGT	4 cut(s)	26, 719, 1314, 1991
Bst6I	CTCTTC	4 cut(s)	130, 574, 1976, 2013
BstACI	GRCGYC	2 cut(s)	216, 249
BstBI	TTCGAA	1 cut(s)	2366
BstC8I	GCNNGC	3 cut(s)	20, 90, 1955
BstDEI	CTNAG	9 cut(s)	299, 399, 438, 669, 692, 793, 1374, 1698, 1886
BstDSI	CCRYGG	1 cut(s)	244
BstEII	GGTNACC	1 cut(s)	1178
BstENI	CCTNNNNNAGG	1 cut(s)	2355
BstF5I	GGATG	8 cut(s)	174, 858, 940, 1065, 1232, 1402, 2316, 2326
BstFNI	CGCG	5 cut(s)	155, 189, 246, 377, 628
BstH2I	RGCGCY	1 cut(s)	252
BstHHI	GCGC	7 cut(s)	152, 157, 189, 251, 377, 990, 2224
BstKTI	GATC	7 cut(s)	261, 625, 801, 805, 1138, 1291, 2179
BstMAI	GTCTC	2 cut(s)	572, 732
BstMBI	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
BstMWI	GCNNNNNNNGC	8 cut(s)	94, 304, 404, 1757, 1993, 2036, 2066, 2230
BstNI	CCWGG	3 cut(s)	295, 1448, 2352
BstNSI	RCATGY	1 cut(s)	1939
BstPI	GGTNACC	1 cut(s)	1178
BstSCI	CCNGG	7 cut(s)	157, 293, 1446, 1789, 2338, 2350, 2440
BstSFI	CTRYAG	4 cut(s)	660, 903, 1557, 1722
BstSLI	GKGCMC	1 cut(s)	2441
BstUI	CGCG	5 cut(s)	155, 189, 246, 377, 628
BstV1I	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1357, 1708, 2206, 2371
BstV2I	GAAGAC	3 cut(s)	375, 753, 948
BstX2I	RGATCY	1 cut(s)	1288
BstXI	CCANNNNNNTGG	1 cut(s)	1243
BstYI	RGATCY	1 cut(s)	1288
Bsu15I	ATCGAT	1 cut(s)	801
BsuTUI	ATCGAT	1 cut(s)	801
BtgI	CCRYGG	1 cut(s)	244
BtrI	CACGTC	1 cut(s)	640
BtsCI	GGATG	8 cut(s)	174, 858, 940, 1065, 1232, 1402, 2316, 2326
BtsI	GCAGTG	1 cut(s)	393
BtsIMutI	CAGTG	4 cut(s)	393, 801, 1155, 1243
BveI	ACCTGC	1 cut(s)	1594
Cac8I	GCNNGC	3 cut(s)	20, 90, 1955
CciI	TCATGA	1 cut(s)	349
CfoI	GCGC	7 cut(s)	152, 157, 189, 251, 377, 990, 2224
Cfr13I	GGNCC	6 cut(s)	226, 1499, 1728, 2336, 2437, 2438
Cfr42I	CCGCGG	1 cut(s)	247
ClaI	ATCGAT	1 cut(s)	801
CseI	GACGC	4 cut(s)	205, 617, 902, 1388
Csp6I	GTAC	9 cut(s)	37, 601, 689, 835, 1043, 1315, 1794, 1933, 2412
CspCI	CAANNNNNGTGG	2 cut(s)	2058, 2093
CviAII	CATG	6 cut(s)	59, 81, 350, 1211, 1936, 2032
CviQI	GTAC	9 cut(s)	37, 601, 689, 835, 1043, 1315, 1794, 1933, 2412
DdeI	CTNAG	9 cut(s)	299, 399, 438, 669, 692, 793, 1374, 1698, 1886
DinI	GGCGCC	1 cut(s)	250
DpnI	GATC	7 cut(s)	260, 624, 800, 804, 1137, 1290, 2178
DpnII	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
DraI	TTTAAA	2 cut(s)	1359, 2047
EaeI	YGGCCR	1 cut(s)	291
Eam1104I	CTCTTC	4 cut(s)	130, 574, 1976, 2013
EarI	CTCTTC	4 cut(s)	130, 574, 1976, 2013
EciI	GGCGGA	1 cut(s)	865
Ecl136II	GAGCTC	1 cut(s)	1031
Eco130I	CCWWGG	4 cut(s)	51, 1183, 1898, 2388
Eco147I	AGGCCT	3 cut(s)	869, 2233, 2350
Eco24I	GRGCYC	2 cut(s)	1033, 2441
Eco32I	GATATC	1 cut(s)	1543
Eco47I	GGWCC	3 cut(s)	226, 1499, 1728
Eco53kI	GAGCTC	1 cut(s)	1031
Eco57I	CTGAAG	2 cut(s)	463, 2436
Eco88I	CYCGRG	2 cut(s)	72, 2123
Eco91I	GGTNACC	1 cut(s)	1178
EcoICRI	GAGCTC	1 cut(s)	1031
EcoNI	CCTNNNNNAGG	1 cut(s)	2355
EcoO109I	RGGNCCY	2 cut(s)	2437, 2438
EcoO65I	GGTNACC	1 cut(s)	1178
EcoRII	CCWGG	3 cut(s)	293, 1446, 2350
EcoRV	GATATC	1 cut(s)	1543
EcoT14I	CCWWGG	4 cut(s)	51, 1183, 1898, 2388
EcoT38I	GRGCYC	2 cut(s)	1033, 2441
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	4 cut(s)	51, 1183, 1898, 2388
Esp3I	CGTCTC	1 cut(s)	572
FaeI	CATG	6 cut(s)	62, 84, 353, 1214, 1939, 2035
FaqI	GGGAC	4 cut(s)	212, 865, 1508, 1741
FatI	CATG	6 cut(s)	58, 80, 349, 1210, 1935, 2031
FauNDI	CATATG	1 cut(s)	1306
FblI	GTMKAC	3 cut(s)	219, 504, 631
FokI	GGATG	8 cut(s)	181, 865, 947, 1072, 1219, 1409, 2323, 2333
FriOI	GRGCYC	2 cut(s)	1033, 2441
FspBI	CTAG	3 cut(s)	47, 558, 1016
GlaI	GCGC	7 cut(s)	151, 156, 188, 250, 376, 989, 2223
GsaI	CCCAGC	1 cut(s)	97
HaeII	RGCGCY	1 cut(s)	252
HapII	CCGG	5 cut(s)	159, 1790, 2339, 2415, 2442
HgaI	GACGC	4 cut(s)	205, 617, 902, 1388
HhaI	GCGC	7 cut(s)	152, 157, 189, 251, 377, 990, 2224
Hin1I	GRCGYC	2 cut(s)	216, 249
Hin1II	CATG	6 cut(s)	62, 84, 353, 1214, 1939, 2035
Hin6I	GCGC	7 cut(s)	150, 155, 187, 249, 375, 988, 2222
HinP1I	GCGC	7 cut(s)	150, 155, 187, 249, 375, 988, 2222
HincII	GTYRAC	1 cut(s)	220
HindII	GTYRAC	1 cut(s)	220
HindIII	AAGCTT	3 cut(s)	86, 1010, 1749
HinfI	GANTC	9 cut(s)	1151, 1247, 1433, 1460, 1465, 1478, 1799, 1811, 1826
HpaII	CCGG	5 cut(s)	159, 1790, 2339, 2415, 2442
HphI	GGTGA	8 cut(s)	22, 265, 436, 1181, 1190, 1421, 2405, 2435
Hpy166II	GTNNAC	4 cut(s)	220, 505, 632, 1317
Hpy188III	TCNNGA	9 cut(s)	72, 256, 350, 1034, 1295, 1457, 1538, 2125, 2454
Hpy8I	GTNNAC	4 cut(s)	220, 505, 632, 1317
Hpy99I	CGWCG	3 cut(s)	200, 221, 1766
HpyCH4III	ACNGT	4 cut(s)	26, 719, 1314, 1991
HpyCH4IV	ACGT	4 cut(s)	581, 639, 974, 1354
HpyF10VI	GCNNNNNNNGC	8 cut(s)	94, 304, 404, 1757, 1993, 2036, 2066, 2230
HpyF3I	CTNAG	9 cut(s)	299, 399, 438, 669, 692, 793, 1374, 1698, 1886
HpySE526I	ACGT	4 cut(s)	581, 639, 974, 1354
Hsp92I	GRCGYC	2 cut(s)	216, 249
Hsp92II	CATG	6 cut(s)	62, 84, 353, 1214, 1939, 2035
HspAI	GCGC	7 cut(s)	150, 155, 187, 249, 375, 988, 2222
KasI	GGCGCC	1 cut(s)	248
KpnI	GGTACC	1 cut(s)	2415
KspI	CCGCGG	1 cut(s)	247
Kzo9I	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
LguI	GCTCTTC	1 cut(s)	574
LmnI	GCTCC	7 cut(s)	55, 74, 454, 613, 967, 985, 1792
Lsp1109I	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1357, 1708, 2206, 2371
MaeI	CTAG	3 cut(s)	47, 558, 1016
MaeII	ACGT	4 cut(s)	581, 639, 974, 1354
MaeIII	GTNAC	3 cut(s)	1178, 2008, 2423
MalI	GATC	7 cut(s)	260, 624, 800, 804, 1137, 1290, 2178
MbiI	CCGCTC	1 cut(s)	69
MboI	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
MflI	RGATCY	1 cut(s)	1288
MhlI	GDGCHC	3 cut(s)	1033, 1789, 2441
MluCI	AATT	8 cut(s)	113, 744, 1515, 1659, 1686, 2042, 2052, 2362
Mly113I	GGCGCC	1 cut(s)	249
MlyI	GAGTC	2 cut(s)	1241, 1805
MmeI	TCCRAC	2 cut(s)	146, 286
MroXI	GAANNNNTTC	2 cut(s)	1290, 2449
MseI	TTAA	6 cut(s)	597, 654, 785, 1358, 2025, 2046
MslI	CAYNNNNRTG	1 cut(s)	720
MspA1I	CMGCKG	2 cut(s)	246, 1348
MspI	CCGG	5 cut(s)	159, 1790, 2339, 2415, 2442
MspR9I	CCNGG	7 cut(s)	159, 295, 1448, 1791, 2340, 2352, 2442
MteI	GCGCNGCGC	1 cut(s)	153
Mva1269I	GAATGC	3 cut(s)	290, 409, 1699
MvaI	CCWGG	3 cut(s)	295, 1448, 2352
MvnI	CGCG	5 cut(s)	155, 189, 246, 377, 628
MwoI	GCNNNNNNNGC	8 cut(s)	94, 304, 404, 1757, 1993, 2036, 2066, 2230
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	4 cut(s)	159, 1791, 2340, 2442
NdeI	CATATG	1 cut(s)	1306
NdeII	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
NlaIII	CATG	6 cut(s)	62, 84, 353, 1214, 1939, 2035
NlaIV	GGNNCC	9 cut(s)	228, 250, 450, 609, 1729, 1988, 2413, 2439, 2440
NmeAIII	GCCGAG	1 cut(s)	1882
NmuCI	GTSAC	3 cut(s)	1178, 2008, 2423
NspI	RCATGY	1 cut(s)	1939
NspV	TTCGAA	1 cut(s)	2366
PaeR7I	CTCGAG	1 cut(s)	2123
PagI	TCATGA	1 cut(s)	349
PceI	AGGCCT	3 cut(s)	869, 2233, 2350
PciI	ACATGT	1 cut(s)	1935
PciSI	GCTCTTC	1 cut(s)	574
PcsI	WCGNNNNNNNCGW	4 cut(s)	138, 204, 213, 2101
PctI	GAATGC	3 cut(s)	290, 409, 1699
PdmI	GAANNNNTTC	2 cut(s)	1290, 2449
PfeI	GAWTC	7 cut(s)	1151, 1433, 1460, 1465, 1478, 1799, 1826
PflFI	GACNNNGTC	1 cut(s)	224
PleI	GAGTC	2 cut(s)	1241, 1805
PluTI	GGCGCC	1 cut(s)	252
PpsI	GAGTC	2 cut(s)	1241, 1805
PscI	ACATGT	1 cut(s)	1935
PshBI	ATTAAT	1 cut(s)	2025
PsiI	TTATAA	1 cut(s)	495
Psp124BI	GAGCTC	1 cut(s)	1033
Psp1406I	AACGTT	1 cut(s)	1354
Psp6I	CCWGG	3 cut(s)	293, 1446, 2350
PspEI	GGTNACC	1 cut(s)	1178
PspFI	CCCAGC	1 cut(s)	93
PspGI	CCWGG	3 cut(s)	293, 1446, 2350
PspN4I	GGNNCC	9 cut(s)	228, 250, 450, 609, 1729, 1988, 2413, 2439, 2440
PspOMI	GGGCCC	1 cut(s)	2437
PspPI	GGNCC	6 cut(s)	226, 1499, 1728, 2336, 2437, 2438
PsrI	GAACNNNNNNTAC	2 cut(s)	451, 483
PstI	CTGCAG	1 cut(s)	1726
PsuI	RGATCY	1 cut(s)	1288
PsyI	GACNNNGTC	1 cut(s)	224
RsaI	GTAC	9 cut(s)	38, 602, 690, 836, 1044, 1316, 1795, 1934, 2413
RsaNI	GTAC	9 cut(s)	37, 601, 689, 835, 1043, 1315, 1794, 1933, 2412
RseI	CAYNNNNRTG	1 cut(s)	720
SacI	GAGCTC	1 cut(s)	1033
SacII	CCGCGG	1 cut(s)	247
SalI	GTCGAC	1 cut(s)	218
SapI	GCTCTTC	1 cut(s)	574
SaqAI	TTAA	6 cut(s)	597, 654, 785, 1358, 2025, 2046
Sau3AI	GATC	7 cut(s)	258, 622, 798, 802, 1135, 1288, 2176
Sau96I	GGNCC	6 cut(s)	226, 1499, 1728, 2336, 2437, 2438
ScaI	AGTACT	1 cut(s)	38
SchI	GAGTC	2 cut(s)	1241, 1805
ScrFI	CCNGG	7 cut(s)	159, 295, 1448, 1791, 2340, 2352, 2442
SduI	GDGCHC	3 cut(s)	1033, 1789, 2441
SfcI	CTRYAG	4 cut(s)	660, 903, 1557, 1722
SfoI	GGCGCC	1 cut(s)	250
Sfr274I	CTCGAG	1 cut(s)	2123
Sfr303I	CCGCGG	1 cut(s)	247
SfuI	TTCGAA	1 cut(s)	2366
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	3 cut(s)	226, 1499, 1728
SlaI	CTCGAG	1 cut(s)	2123
SmiMI	CAYNNNNRTG	1 cut(s)	720
SmlI	CTYRAG	2 cut(s)	1032, 2123
SmoI	CTYRAG	2 cut(s)	1032, 2123
Sse9I	AATT	8 cut(s)	113, 744, 1515, 1659, 1686, 2042, 2052, 2362
SseBI	AGGCCT	3 cut(s)	869, 2233, 2350
SspDI	GGCGCC	1 cut(s)	248
SspMI	CTAG	3 cut(s)	47, 558, 1016
SstI	GAGCTC	1 cut(s)	1033
StuI	AGGCCT	3 cut(s)	869, 2233, 2350
StyD4I	CCNGG	7 cut(s)	157, 293, 1446, 1789, 2338, 2350, 2440
StyI	CCWWGG	4 cut(s)	51, 1183, 1898, 2388
TaaI	ACNGT	4 cut(s)	26, 719, 1314, 1991
TaiI	ACGT	4 cut(s)	584, 642, 977, 1357
TasI	AATT	8 cut(s)	113, 744, 1515, 1659, 1686, 2042, 2052, 2362
TatI	WGTACW	5 cut(s)	36, 600, 688, 1314, 1932
TauI	GCSGC	3 cut(s)	155, 249, 375
TfiI	GAWTC	7 cut(s)	1151, 1433, 1460, 1465, 1478, 1799, 1826
Tru1I	TTAA	6 cut(s)	597, 654, 785, 1358, 2025, 2046
Tru9I	TTAA	6 cut(s)	597, 654, 785, 1358, 2025, 2046
TscAI	CASTG	4 cut(s)	400, 801, 1162, 1243
TseFI	GTSAC	3 cut(s)	1178, 2008, 2423
TseI	GCWGC	9 cut(s)	184, 201, 554, 767, 770, 1345, 1721, 2219, 2384
Tsp45I	GTSAC	3 cut(s)	1178, 2008, 2423
TspGWI	ACGGA	7 cut(s)	867, 1060, 1512, 1724, 1772, 2285, 2323
TspRI	CASTG	4 cut(s)	400, 801, 1162, 1243
Tth111I	GACNNNGTC	1 cut(s)	224
VpaK11BI	GGWCC	3 cut(s)	226, 1499, 1728
VspI	ATTAAT	1 cut(s)	2025
XagI	CCTNNNNNAGG	1 cut(s)	2355
XceI	RCATGY	1 cut(s)	1939
XhoI	CTCGAG	1 cut(s)	2123
XmiI	GTMKAC	3 cut(s)	219, 504, 631
XmnI	GAANNNNTTC	2 cut(s)	1290, 2449
XspI	CTAG	3 cut(s)	47, 558, 1016
ZrmI	AGTACT	1 cut(s)	38

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.