pycom10g07800

Belongs to the CGI121 TPRKB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007405
Physical Location & Seq
Forward (+)
9511255 .. 9512431
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGGGCGCCAACACAGATAGAGAAACGTTTGACCAGTGCAGTGCAGAAAGTCCGGAGCTTGAAGACGCAACAATGAAGGACTTTCAGGTCAATGGGAACACACCCTCTCTTGCTATCTTCACTCAAGTCACCAACTCCAAGGAGCTGCTTGATTTGATGCAATCAGGGACACTAAACGCAGAAGTCACTTTCCTGAATGCTTCGCTTATCCCAGATGTTTTCCCTTTACTTGCAGCTGCACATAAGACTCTCGTATCTAAGTCCAGAGAGTTACTGACAACACGCACTCAGGATCCAAGCATATGTGGGATTTCTGAAATCTCAACCTATGTGCTTGCCACTCGTTTCAATGCTTCTTCTGATGAGGTAAGATCACTGTCTCATGAAATGCCTGGTAGATCAAGCGTTACATTGCTTCAGTATATCCGAAAATTGTTAGCCCTTTCGACTTTAATCATGTTCGTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CGI-121

Protein Analysis

157

Amino Acids

17.1

Weight (kDa)

4.83

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGI-121 PF08617 40 - 96 1.2e-10 Kinase binding protein CGI-121
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 469
AasI GACNNNNNNGTC 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 52
AclI AACGTT 1 cut(s) 26
AclWI GGATC 2 cut(s) 287, 300
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 6
AgsI TTSAA 2 cut(s) 62, 349
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 3 cut(s) 58, 145, 236
AluI AGCT 3 cut(s) 58, 145, 236
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 2 cut(s) 287, 300
Aor13HI TCCGGA 1 cut(s) 52
ApeKI GCWGC 3 cut(s) 145, 233, 236
AspLEI GCGC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 121
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BamHI GGATCC 1 cut(s) 292
BanI GGYRCC 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 3 cut(s) 132, 223, 245
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 384
BfoI RGCGCY 1 cut(s) 9
BisI GCNGC 3 cut(s) 146, 234, 237
BlsI GCNGC 3 cut(s) 147, 235, 238
Bme1390I CCNGG 1 cut(s) 393
BmiI GGNNCC 2 cut(s) 7, 294
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 52
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 410
BseAI TCCGGA 1 cut(s) 52
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 138
BseMI GCAATG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 34
BseXI GCAGC 3 cut(s) 132, 223, 245
BsgI GTGCAG 3 cut(s) 58, 63, 222
BshNI GGYRCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 53
BslFI GGGAC 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 202
Bsp13I TCCGGA 1 cut(s) 52
Bsp143I GATC 3 cut(s) 292, 371, 398
BspCNI CTCAG 1 cut(s) 301
BspEI TCCGGA 1 cut(s) 52
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 7, 294
BspPI GGATC 2 cut(s) 287, 300
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 410
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 3 cut(s) 292, 371, 398
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 6
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 258, 288
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 3 cut(s) 295, 374, 401
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 3 cut(s) 292, 371, 398
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 391
BstV1I GCAGC 3 cut(s) 132, 223, 245
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 3 cut(s) 41, 46, 374
Cac8I GCNNGC 1 cut(s) 336
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 8
CseI GACGC 1 cut(s) 74
CviAII CATG 2 cut(s) 383, 457
CviJI RGCY 4 cut(s) 58, 145, 236, 440
CviKI_1 RGCY 4 cut(s) 58, 145, 236, 440
DdeI CTNAG 2 cut(s) 258, 288
DinI GGCGCC 1 cut(s) 7
DpnI GATC 3 cut(s) 294, 373, 400
DpnII GATC 3 cut(s) 292, 371, 398
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 138
EgeI GGCGCC 1 cut(s) 7
EheI GGCGCC 1 cut(s) 7
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 2 cut(s) 386, 460
FaiI YATR 8 cut(s) 243, 302, 304, 330, 384, 423, 458, 469
FaqI GGGAC 1 cut(s) 181
FatI CATG 2 cut(s) 382, 456
FauNDI CATATG 1 cut(s) 302
Fnu4HI GCNGC 3 cut(s) 146, 234, 237
Fsp4HI GCNGC 3 cut(s) 146, 234, 237
GlaI GCGC 1 cut(s) 7
GluI GCNGC 3 cut(s) 146, 234, 237
HaeII RGCGCY 1 cut(s) 9
HapII CCGG 1 cut(s) 53
HgaI GACGC 1 cut(s) 74
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 2 cut(s) 386, 460
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HinfI GANTC 1 cut(s) 247
HpaII CCGG 1 cut(s) 53
HphI GGTGA 1 cut(s) 121
Hpy188I TCNGA 3 cut(s) 316, 361, 428
Hpy188III TCNNGA 5 cut(s) 53, 193, 264, 290, 383
HpyAV CCTTC 1 cut(s) 70
HpyCH4III ACNGT 1 cut(s) 378
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 5 cut(s) 39, 44, 160, 233, 239
HpyF3I CTNAG 2 cut(s) 258, 288
HpySE526I ACGT 1 cut(s) 26
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 2 cut(s) 386, 460
HspAI GCGC 1 cut(s) 6
KasI GGCGCC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 52
Kzo9I GATC 3 cut(s) 292, 371, 398
LmnI GCTCC 2 cut(s) 55, 142
Lsp1109I GCAGC 3 cut(s) 132, 223, 245
LweI GCATC 1 cut(s) 147
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 4 cut(s) 127, 184, 270, 406
MalI GATC 3 cut(s) 294, 373, 400
MboI GATC 3 cut(s) 292, 371, 398
MboII GAAGA 3 cut(s) 74, 109, 348
MflI RGATCY 1 cut(s) 292
MluCI AATT 1 cut(s) 431
Mly113I GGCGCC 1 cut(s) 6
MlyI GAGTC 1 cut(s) 241
MnlI CCTC 2 cut(s) 115, 358
MroI TCCGGA 1 cut(s) 52
MseI TTAA 1 cut(s) 452
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 393
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 1 cut(s) 393
NarI GGCGCC 1 cut(s) 6
NdeI CATATG 1 cut(s) 302
NdeII GATC 3 cut(s) 292, 371, 398
NlaIII CATG 2 cut(s) 386, 460
NlaIV GGNNCC 2 cut(s) 7, 294
NmuCI GTSAC 2 cut(s) 127, 184
PagI TCATGA 1 cut(s) 382
PctI GAATGC 1 cut(s) 202
PkrI GCNGC 3 cut(s) 147, 235, 238
PleI GAGTC 1 cut(s) 241
PluTI GGCGCC 1 cut(s) 9
PpsI GAGTC 1 cut(s) 241
PsiI TTATAA 1 cut(s) 469
Psp1406I AACGTT 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 2 cut(s) 7, 294
PsuI RGATCY 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 236
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 3 cut(s) 146, 234, 237
Sau3AI GATC 3 cut(s) 292, 371, 398
SchI GAGTC 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 393
SetI ASST 7 cut(s) 29, 60, 90, 147, 238, 329, 369
SfaNI GCATC 1 cut(s) 147
SfoI GGCGCC 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 123
SmoI CTYRAG 1 cut(s) 123
Sse9I AATT 1 cut(s) 431
SspDI GGCGCC 1 cut(s) 5
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 378
TaiI ACGT 1 cut(s) 29
TaqI TCGA 1 cut(s) 446
TasI AATT 1 cut(s) 431
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TscAI CASTG 3 cut(s) 41, 46, 381
TseFI GTSAC 2 cut(s) 127, 184
TseI GCWGC 3 cut(s) 145, 233, 236
Tsp45I GTSAC 2 cut(s) 127, 184
TspDTI ATGAA 2 cut(s) 89, 399
TspRI CASTG 3 cut(s) 41, 46, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.