pycom10g13790

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015193
Physical Location & Seq
Forward (+)
17309573 .. 17310951
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
ATGGCTTCCGACGATCCCAAAGCCGAAGAAGCAGAACCACCGCCGTCGTCTTGGAAGGAGCGCATCGTCTTCCCCACTCTCCTAGCTGGGATTCTCGGCGGAGGAGCTGGGTTGGTCTCCAAGCATAGAAAAGTCATGGGTCTCCCGACCGGTTGCGCTACCTATGCTGCCAATTTCGCCATAGTTACCGCTTGTTACTGCGGGGCTCGTGAATATGTGGGAGTAACTCGAAAAACTGGACCTGATGATCTGGTGAACTCTGCAATTGCTGGGTTTGGTACTGGTGCCATTCTCGGGCGTCTTCAGGGTGGTCAAATTGGTGCTGTCCGCTACTCAATCATCTTTACCGTTGTTGGGACAACTGTGGATTATGCTACAATTAGACTAAGACCTGTGTTAAAGAGCTACAAGGAATCTATGCTTGGGAGTAATGACGGTTCACAGAAGAATGACGGTTGGCTGAAAATGCCTGACTGGTCTCCTATCAAAGTACTTGATGAGGAAGCCCTTGCTGCAAAACAGGCTCGTGAACAAAAGGTGCATGCACAGAGGGTAGCACTTGGTAACCTAAGCAAAGAAGAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

196

Amino Acids

20.74

Weight (kDa)

8.73

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 49 - 118 2.2e-07 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25120
fragaria_vesca FvH4_2g18530
malus_domestica MD05G1171500.v1.1 MD10G1159700.v1.1
prunus_persica Prupe.8G198800_v2.0.a1
pyrus_communis pycom05g15820 pycom10g13790
rosa_chinensis RchiOBHm_Chr6g0284091
rosa_laevigata RLG00000012770
rosa_roxburghii Rroxscaffold_7G00184940
rosa_rugosa Rorug06G0160600
rosa_samantha Rh6AG273400 Rh6BG274100 Rh6CG275500 Rh6DG268600
rosa_wichuraiana Rw6G023530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 284
AciI CCGC 5 cut(s) 41, 99, 189, 201, 328
AclWI GGATC 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 298
AfaI GTAC 2 cut(s) 280, 492
AfiI CCNNNNNNNGG 2 cut(s) 294, 354
AgeI ACCGGT 1 cut(s) 149
AjuI GAANNNNNNNTTGG 2 cut(s) 405, 437
AluBI AGCT 3 cut(s) 86, 107, 405
AluI AGCT 3 cut(s) 86, 107, 405
Alw26I GTCTC 3 cut(s) 121, 146, 483
AlwI GGATC 1 cut(s) 8
Ama87I CYCGRG 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 167, 512
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 2 cut(s) 63, 158
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 208
BauI CACGAG 2 cut(s) 207, 525
BbsI GAAGAC 2 cut(s) 61, 293
BbvI GCAGC 2 cut(s) 154, 499
BceAI ACGGC 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 121, 146, 483
BfaI CTAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 168, 513
BlsI GCNGC 2 cut(s) 169, 514
BmcAI AGTACT 1 cut(s) 492
Bme18I GGWCC 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 286
BmsI GCATC 1 cut(s) 72
BpiI GAAGAC 2 cut(s) 61, 293
Bpu10I CCTNAGC 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 298
BsaI GGTCTC 3 cut(s) 121, 146, 483
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 354
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 241, 286, 479
BseLI CCNNNNNNNGG 2 cut(s) 294, 354
BseNI ACTGG 3 cut(s) 241, 286, 479
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 2 cut(s) 154, 499
BseYI CCCAGC 3 cut(s) 86, 107, 269
Bsh1285I CGRYCG 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 284
BshTI ACCGGT 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 2 cut(s) 294, 354
BsmAI GTCTC 3 cut(s) 121, 146, 483
BsmFI GGGAC 1 cut(s) 370
Bso31I GGTCTC 3 cut(s) 121, 146, 483
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 13, 247
BspACI CCGC 5 cut(s) 41, 99, 189, 201, 328
BspLI GGNNCC 1 cut(s) 286
BspPI GGATC 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 3 cut(s) 121, 146, 483
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 3 cut(s) 241, 286, 479
BssAI RCCGGY 1 cut(s) 149
BssMI GATC 2 cut(s) 13, 247
BssNI GRCGYC 1 cut(s) 298
BssSI CACGAG 2 cut(s) 207, 525
Bst2BI CACGAG 2 cut(s) 207, 525
Bst4CI ACNGT 4 cut(s) 349, 364, 437, 455
Bst6I CTCTTC 1 cut(s) 573
BstACI GRCGYC 1 cut(s) 298
BstC8I GCNNGC 1 cut(s) 543
BstDEI CTNAG 2 cut(s) 386, 569
BstEII GGTNACC 1 cut(s) 563
BstHHI GCGC 2 cut(s) 63, 158
BstKTI GATC 2 cut(s) 16, 250
BstMAI GTCTC 3 cut(s) 121, 146, 483
BstMBI GATC 2 cut(s) 13, 247
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 6 cut(s) 29, 164, 176, 466, 512, 521
BstNSI RCATGY 1 cut(s) 545
BstPI GGTNACC 1 cut(s) 563
BstV1I GCAGC 2 cut(s) 154, 499
BstV2I GAAGAC 2 cut(s) 61, 293
Cac8I GCNNGC 1 cut(s) 543
CfoI GCGC 2 cut(s) 63, 158
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 1 cut(s) 287
Csp6I GTAC 2 cut(s) 279, 491
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 2 cut(s) 136, 542
CviJI RGCY 9 cut(s) 5, 23, 86, 107, 206, 405, 460, 506, 524
CviKI_1 RGCY 9 cut(s) 5, 23, 86, 107, 206, 405, 460, 506, 524
CviQI GTAC 2 cut(s) 279, 491
DdeI CTNAG 2 cut(s) 386, 569
DpnI GATC 2 cut(s) 15, 249
DpnII GATC 2 cut(s) 13, 247
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
EciI GGCGGA 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 3 cut(s) 121, 146, 483
Eco47I GGWCC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 293
Eco91I GGTNACC 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 208
FaeI CATG 2 cut(s) 139, 545
FaiI YATR 8 cut(s) 126, 137, 165, 182, 216, 372, 419, 543
FaqI GGGAC 1 cut(s) 370
FatI CATG 2 cut(s) 135, 541
FauI CCCGC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 168, 513
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 2 cut(s) 168, 513
FspBI CTAG 1 cut(s) 83
GlaI GCGC 2 cut(s) 62, 157
GluI GCNGC 2 cut(s) 168, 513
GsaI CCCAGC 3 cut(s) 90, 111, 273
HapII CCGG 1 cut(s) 150
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 63, 158
Hin1I GRCGYC 1 cut(s) 298
Hin1II CATG 2 cut(s) 139, 545
Hin6I GCGC 2 cut(s) 61, 156
HinP1I GCGC 2 cut(s) 61, 156
HinfI GANTC 3 cut(s) 91, 413, 581
HpaII CCGG 1 cut(s) 150
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 3 cut(s) 256, 440, 530
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 3 cut(s) 145, 209, 527
Hpy8I GTNNAC 3 cut(s) 256, 440, 530
Hpy99I CGWCG 2 cut(s) 14, 49
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 4 cut(s) 349, 364, 437, 455
HpyCH4V TGCA 4 cut(s) 263, 515, 541, 545
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 164, 176, 466, 512, 521
HpyF3I CTNAG 2 cut(s) 386, 569
Hsp92I GRCGYC 1 cut(s) 298
Hsp92II CATG 2 cut(s) 139, 545
HspAI GCGC 2 cut(s) 61, 156
Kzo9I GATC 2 cut(s) 13, 247
LmnI GCTCC 2 cut(s) 58, 104
Lsp1109I GCAGC 2 cut(s) 154, 499
LweI GCATC 1 cut(s) 72
MaeI CTAG 1 cut(s) 83
MaeIII GTNAC 4 cut(s) 184, 194, 223, 563
MalI GATC 2 cut(s) 15, 249
MboI GATC 2 cut(s) 13, 247
MboII GAAGA 4 cut(s) 38, 61, 293, 457
MfeI CAATTG 1 cut(s) 264
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 4 cut(s) 172, 264, 315, 378
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 3 cut(s) 95, 493, 543
MseI TTAA 1 cut(s) 398
MspI CCGG 1 cut(s) 150
MunI CAATTG 1 cut(s) 264
MwoI GCNNNNNNNGC 6 cut(s) 29, 164, 176, 466, 512, 521
NdeII GATC 2 cut(s) 13, 247
NlaIII CATG 2 cut(s) 139, 545
NlaIV GGNNCC 1 cut(s) 286
NmeAIII GCCGAG 1 cut(s) 75
NspI RCATGY 1 cut(s) 545
PaeI GCATGC 1 cut(s) 545
PfeI GAWTC 2 cut(s) 91, 413
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 2 cut(s) 169, 514
PspEI GGTNACC 1 cut(s) 563
PspFI CCCAGC 3 cut(s) 86, 107, 269
PspN4I GGNNCC 1 cut(s) 286
PspPI GGNCC 1 cut(s) 239
PsrI GAACNNNNNNTAC 2 cut(s) 421, 453
RsaI GTAC 2 cut(s) 280, 492
RsaNI GTAC 2 cut(s) 279, 491
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 2 cut(s) 168, 513
Sau3AI GATC 2 cut(s) 13, 247
Sau96I GGNCC 1 cut(s) 239
ScaI AGTACT 1 cut(s) 492
SduI GDGCHC 1 cut(s) 208
SetI ASST 8 cut(s) 88, 109, 164, 244, 394, 407, 540, 570
SfaNI GCATC 1 cut(s) 72
SinI GGWCC 1 cut(s) 239
SphI GCATGC 1 cut(s) 545
Sse9I AATT 4 cut(s) 172, 264, 315, 378
SsiI CCGC 5 cut(s) 41, 99, 189, 201, 328
SspMI CTAG 1 cut(s) 83
TaaI ACNGT 4 cut(s) 349, 364, 437, 455
TaqI TCGA 1 cut(s) 229
TasI AATT 4 cut(s) 172, 264, 315, 378
TatI WGTACW 1 cut(s) 490
TfiI GAWTC 2 cut(s) 91, 413
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TseI GCWGC 2 cut(s) 167, 512
VpaK11BI GGWCC 1 cut(s) 239
XceI RCATGY 1 cut(s) 545
XspI CTAG 1 cut(s) 83
ZrmI AGTACT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.