pycom10g14620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009380
Physical Location & Seq
Reverse (-)
17973549 .. 17975426
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGGCCGGTTCGGTTATGCTGCAAAGCCTGTGCTGCATAACCCCACAATCCCCAAATTGTTACAAGAATCAAACCCTAGCTTTCTCCAACTCTCAGCTCTTCTCCTCCAGTTCATCTTTTAGATTAAAGAAACAGACTTTACCGTCAGCTATTCGGATTAAGAGGCGGAGGACTCAGAATCATCGCTCTGTGGTTCCTGTTGTGTTTGCTTCACAGTCCAACTTCTTCAAAGTTCTGCAAACGGCATGGAAGGTTGGGAGAGATGGAGTTGAGGCTGGAACCAATCTTGTCCCGAATTCAGTACCGAGACCAGTTGCAAGGGTTTCTGTGACTATAGTTGCATTGACACTTTCACTGTTTGTGCTCAAATCTTTCCTGTCTACAGCTTTGTTTGCGCTGGCTACGATGGGACTTGTATACTTTATATTCATTGCTTTGAACAAGGATGAAGGGCCCAGGGGGGGTGGAGGCACCACCTCAACGCCAAAGGAGGACGGGATAGACGACAGTCTAGAAGAAGCCAGAAGAATCATGGAGAAGTACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

19.95

Weight (kDa)

10.13

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 142
AccB1I GGYRCC 1 cut(s) 470
AccI GTMKAC 2 cut(s) 380, 417
AciI CCGC 1 cut(s) 166
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 295
AfaI GTAC 2 cut(s) 303, 542
AfiI CCNNNNNNNGG 1 cut(s) 461
AgsI TTSAA 2 cut(s) 229, 439
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 4 cut(s) 80, 97, 149, 386
AluI AGCT 4 cut(s) 80, 97, 149, 386
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 1 cut(s) 301
AoxI GGCC 2 cut(s) 3, 452
ApaI GGGCCC 1 cut(s) 456
ApeKI GCWGC 2 cut(s) 19, 33
ApoI RAATTY 1 cut(s) 295
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 2 cut(s) 452, 453
BaeGI GKGCMC 1 cut(s) 456
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 6, 20
BccI CCATC 2 cut(s) 257, 400
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 2 cut(s) 77, 512
BfmI CTRYAG 2 cut(s) 333, 381
BisI GCNGC 2 cut(s) 20, 34
BlsI GCNGC 2 cut(s) 21, 35
Bme1390I CCNGG 1 cut(s) 457
BmgT120I GGNCC 2 cut(s) 452, 453
BmiI GGNNCC 4 cut(s) 195, 280, 454, 472
BmrFI CCNGG 1 cut(s) 457
BoxI GACNNNNGTC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 91
BsaI GGTCTC 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 455, 456
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 2 cut(s) 108, 311
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 2 cut(s) 455, 456
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 461
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 107, 188
BseNI ACTGG 2 cut(s) 108, 311
BseRI GAGGAG 1 cut(s) 94
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 2 cut(s) 6, 20
BshFI GGCC 2 cut(s) 5, 454
BshNI GGYRCC 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 1 cut(s) 6
BslFI GGGAC 2 cut(s) 275, 423
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 2 cut(s) 275, 423
BsnI GGCC 2 cut(s) 5, 454
Bso31I GGTCTC 1 cut(s) 301
Bsp120I GGGCCC 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 366, 456
BspACI CCGC 1 cut(s) 166
BspANI GGCC 2 cut(s) 5, 454
BspCNI CTCAG 2 cut(s) 106, 187
BspLI GGNNCC 4 cut(s) 195, 280, 454, 472
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 2 cut(s) 108, 311
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 2 cut(s) 455, 456
BssNAI GTATAC 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 418
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 4 cut(s) 144, 216, 357, 509
Bst6I CTCTTC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 93, 174
BstF5I GGATG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 397
BstMAI GTCTC 1 cut(s) 301
BstMWI GCNNNNNNNGC 2 cut(s) 33, 392
BstNI CCWGG 1 cut(s) 457
BstPAI GACNNNNGTC 1 cut(s) 507
BstSCI CCNGG 1 cut(s) 455
BstSFI CTRYAG 2 cut(s) 333, 381
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 6, 20
BstZ17I GTATAC 1 cut(s) 418
BsuRI GGCC 2 cut(s) 5, 454
BtgZI GCGATG 1 cut(s) 167
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 1 cut(s) 399
CfoI GCGC 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 2 cut(s) 452, 453
Csp6I GTAC 2 cut(s) 302, 541
CviAII CATG 2 cut(s) 246, 532
CviQI GTAC 2 cut(s) 302, 541
DdeI CTNAG 2 cut(s) 93, 174
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 456
FaeI CATG 2 cut(s) 249, 535
FaiI YATR 7 cut(s) 17, 38, 247, 335, 418, 425, 533
FaqI GGGAC 2 cut(s) 275, 423
FatI CATG 2 cut(s) 245, 531
FblI GTMKAC 2 cut(s) 380, 417
Fnu4HI GCNGC 2 cut(s) 20, 34
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 2 cut(s) 20, 34
FspBI CTAG 2 cut(s) 77, 512
GlaI GCGC 1 cut(s) 396
GluI GCNGC 2 cut(s) 20, 34
GsuI CTGGAG 1 cut(s) 91
HaeIII GGCC 2 cut(s) 5, 454
HapII CCGG 1 cut(s) 6
HhaI GCGC 1 cut(s) 397
Hin1II CATG 2 cut(s) 249, 535
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HinfI GANTC 4 cut(s) 67, 172, 178, 528
HpaII CCGG 1 cut(s) 6
Hpy166II GTNNAC 2 cut(s) 381, 418
Hpy188I TCNGA 2 cut(s) 156, 177
Hpy188III TCNNGA 2 cut(s) 292, 512
Hpy8I GTNNAC 2 cut(s) 381, 418
HpyAV CCTTC 2 cut(s) 244, 443
HpyCH4III ACNGT 4 cut(s) 144, 216, 357, 509
HpyCH4V TGCA 5 cut(s) 22, 36, 238, 317, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 392
HpyF3I CTNAG 2 cut(s) 93, 174
Hsp92II CATG 2 cut(s) 249, 535
HspAI GCGC 1 cut(s) 395
LguI GCTCTTC 1 cut(s) 104
Lsp1109I GCAGC 2 cut(s) 6, 20
MaeI CTAG 2 cut(s) 77, 512
MaeIII GTNAC 2 cut(s) 59, 328
MboII GAAGA 4 cut(s) 91, 217, 527, 537
MhlI GDGCHC 2 cut(s) 366, 456
MluCI AATT 2 cut(s) 55, 295
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 2 cut(s) 111, 243
MnlI CCTC 7 cut(s) 115, 156, 162, 265, 461, 484, 487
MseI TTAA 2 cut(s) 125, 159
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 457
MvaI CCWGG 1 cut(s) 457
MwoI GCNNNNNNNGC 2 cut(s) 33, 392
NlaIII CATG 2 cut(s) 249, 535
NlaIV GGNNCC 4 cut(s) 195, 280, 454, 472
NmuCI GTSAC 1 cut(s) 328
PasI CCCWGGG 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PfeI GAWTC 3 cut(s) 67, 178, 528
PkrI GCNGC 2 cut(s) 21, 35
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PshAI GACNNNNGTC 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 4 cut(s) 195, 280, 454, 472
PspOMI GGGCCC 1 cut(s) 452
PspPI GGNCC 2 cut(s) 452, 453
RsaI GTAC 2 cut(s) 303, 542
RsaNI GTAC 2 cut(s) 302, 541
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 2 cut(s) 125, 159
SatI GCNGC 2 cut(s) 20, 34
Sau96I GGNCC 2 cut(s) 452, 453
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 2 cut(s) 366, 456
SetI ASST 6 cut(s) 82, 99, 151, 255, 388, 479
SfcI CTRYAG 2 cut(s) 333, 381
Sse9I AATT 2 cut(s) 55, 295
SsiI CCGC 1 cut(s) 166
SspMI CTAG 2 cut(s) 77, 512
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 4 cut(s) 144, 216, 357, 509
TasI AATT 2 cut(s) 55, 295
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 3 cut(s) 67, 178, 528
Tru1I TTAA 2 cut(s) 125, 159
Tru9I TTAA 2 cut(s) 125, 159
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 2 cut(s) 19, 33
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 3 cut(s) 102, 418, 462
TspRI CASTG 1 cut(s) 360
XapI RAATTY 1 cut(s) 295
XbaI TCTAGA 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XmiI GTMKAC 2 cut(s) 380, 417
XspI CTAG 2 cut(s) 77, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.