Rorug06G0173700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009380
Physical Location & Seq
Reverse (-)
25631057 .. 25635206
4150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0173700.1

Sequence Viewer

Length: 4104 bp
ATGTTTAAAGTCTTATTTTGGAATGCCAGAGGTGCTGGAAGTGAGAATTTTCGATCTGCTATTGCAGACTTGGTTAATTTACATTCTGTGGATATTTTAGCTATCTGTGAACCTCGTGTTCAATTCATTAAGGCTCGAGATACTCTAAAAAAACTTGGTTTTTCTGACTTTAGAGTTGTTGAAGCTGAGGGCTTTTCTGGTGGTATTTGGCTACTATGGAACAGTCTCAAAGTTCATGTTGATTTTATTGACAAAAATTTCCAATCTGTCTCCGTGAAAGTTTCTCTTCCTGGCAAGCCTAGTTGGATGCTCACTGTGATCTATGCTAGCCCTACTAATTCGGTTAGAGCTGATCTGTGGCCTTACCTTGATAATCTCATTGCTGATATTAATCTCCCTTGTATGTTTATTGGAGATTTTAATGAATTGATTTCAATTGATGACAAAACTTGTGGTCCTTTTACTGGAAGGTTTGGTGGTTTCAGGGATTGGGTAAATAGGAATGCCTTGATTGATATGGGATTTCAAGGATCTCGCTTTACCTGGAGTAATAACAGAGTTAAAGAGAGACTGGATAGAGGTTTTTGTAATTGTTCTTGGAGGTCTTCTTTTGCTGAAGCCTTTATCCAACACCTTCCCAAGACTAGATCAGATCACTGCCCTATTTTGATGCAGCTCTACTCCAATAACTTTGTTAATAGAGAGGCTATTCCTTTTAGATTTCAGGCCATGTGGTTCTCTCATGCTAACTATGCTGATTTTGTCTCTTCTACTTGGAATTCTCTTACTGGTGATTTTTCTGCTAAAGTTCAAGCCCTTTCTGTTGCTCTTGCTAAATGGAATAAAGAGGTTTTTGGTCACTTGTTTCACAGGAAAAAAAGAATTTTGGCTAGAATTGGTGGCATTCAAAGGGCCAGAGATAGACATGAAAATCCTTTTTTGATTAATTTGGAAGCTGATCTTATTCAGGAATATGAGACCATCAGAGATCAAGAGAACCTTTTCTGGAGACAAAAATCCAGAGACAAATGGCTGCAAGGTGGAGATAGAAATACCAGGTTCTTCCATCTAACTACTCTTGTTAGAAGAAGGAAAAATAAAATTGAGGGCTTATTTGATAAAAATGGTAACTGGTTCACTGATTCTGCCACTATGAAAAAAATCTCTGTTGATTTTTTTGTTGATCTATTCTCTCTTAAAGATGTTGATGATATCAGATTTATCATTCCTTGGTTGTTCCCTACTATCGATCAGGTCACCTTCGTTAACATCTGTAAGCCTATTACTTTGTTGGAGGTCAAAGAGTCTTTGTTTGCTATTGGTGGCCTCAAAGCTCCTGGCCATGATGGTTTTCCTGCCATCTTTTATCAGAATCATTGGCAAACCTATTCTGGTGAAATTTTTCAAGTTGTTCATGATGCTTTTAGTTCCTGTAAAATTCCCCCTGGCCTGAATCATACTATCATTTCTCTGATTCCTAAAATTGATGACCCTCAGCATATGGCTAACTTTAGACCCATCAGCTTGTGCACTACCATCTATAAAGTCATCTCCAAGATCATTGTTGCTCGTATAAGGCCCTTGATGCAGCACTTAATCAGCCCTAATCAGGTAAGTTATGTTCCTGGCAGGCACATTTCTGATAATGTTATGATTGCTCAAGAAATGCTTTTTAAATTCAGAAGATCTTCTGGAAATTTGGGCTTTTTTGCTTGGAAGGTTGATTTATCTAAAGCTTATGATAGATTGAGATGGGACTTCATTGAGATGGTTTTATATGAAGCTCAATTTCCTCAGTCTCTTGTTAAGCTCATTATGCACTGCATTACGTCAACTAGTTTTCAGATTTGTTTTAATGGAGAGTTGACTTGCTCTTTTGAAGCTCAAAGAGGCATTCGGCAAGGTGACCCTCTCTCACCTTATATTTTTGTTCTTTGTATGGAAAAGCTCTCACATCTTATTCAATCTTGTGTGGACATTGGAAATTGGAAACCTGTTAGAGCTTCTAAATCTGGACCTAAGATTTCCCATCTTTTCTTTGCTGATGATTTAATGCTATTTGCTGAGGCTTCTTCTTATCAGGCTGGTATTCTTAAAAATTGTCTTGATCTGTTTTGTTCCCTCTCTGGACAAGCTGTTAGCTTTGAAAAATCATTGATCTATTGCTCCCCAAATACTTGTAAGGATACTGCTGTCGATGTTAGTCACATTTGTGGGTCTCCTTTGACAAATGATCTTGGCAAATATTTAGGTATGCCCCTCATCCATTCCAGAGTCAATAAACACACCTATGCTGGTATTTTTGACAAGATCCAATGCAGACTTTCTAGTTGGAAAAGCAAGGTTTTGAGTATGGCTGGCAGGTTGGTTTTGGTGCAATCTGTCACCTCTGCTATCCCTAACTATGCCATGCAAACTGCTATGCTGCCCATAAGTCTCTGTGACAAAATTGACAAGCTTAATAGAGACTTCATCTGGGGTGACACTGTTGAGAAGAAGAAGGTCCACTTAGTAAATTGGGACACTGTCTGCCAGCCAAAACAGTTGGGGGGTCTTGGAATAAAAAAGACAGCTGAGATGAATCAAGCCATGCTTGCTAAGGCTAGCTGGAGGATCTCTCAAAAGGATACTGGACTTTGGGCCTCTATGTATGCTGCAAAGTATTTGCAAGGCTCCAATATTACTGATGAAAGCTATAAACCCCCTCCTGATTGTTCCAGTACTTGGAGAAGCATCTCTCATGGTGCTGTGTTGTTGAGAAAATGTTTAAAATGGCGTATTGGGGAGGGCAATTCTATTAGGTTTTGGCTGGATCTTTGGCTTCTCCCTACACCTCTTATAAATTTGGCTCTTCCTTCAGCTGATATAAATATCAATGCAACCATCAATGACTTTTGGACTGAGTCAGGCTGGAACTTGGATCTTCTCTTCTCCATGGTCCCATGTGATATTGTCCATCAGATTATTAATGTCCCTACTGGGTTTGGAAATTGTGGTAATGATTCTTTAATTTGGGGAGCCACTTCCAATGGTTGCTTCACTGTCAAGTCTGCATATAATTCGAATTTTGATTTTCTCAATCTCCAAAATCCTCAATGGATTGCTGCTTGGAAATGTGATATTCCTCCTAAGTTGAAAACTTTTCTCTGGACTGTTCTCCATAGGAAGCTTTTAACTAATGTTCAAAGAACCAGAAGAGGCTTTACCTTGACATCCACATGTCCTATTTGCAATGCTGCTGATGAAACTCTTCTTCACTTGCTCAGAGACTGTCCTAGATCCCTTGCTATCTGGAAAGCTTTTCTTAAGCCTGGTACTATTCTCAACTCTTTCTCTCTTGACTGGAATGGTTGGATTGCTGCTCAATTGCATTGCCAAACTTTGATTCAGAAAAACATTAAATGGTGCAATCTCTTTGTCTTTATCTGTTGGTTTATCTGGAAATGGAGGAATAAGTCTGTCTTTGATCCCAGTTTTACCATGCCTTTCTGTCCGGATAAAGTGATTTGGAATTATGCTGAGGAGTGGATTGGGGCTAATCTCAAAATGAATGCTGATACAGTTTATAGATACACTTTTCTGGCTTGGAAAAGACCTATGGAAAATTTTTGCAAGTTAAACATTGATGGGACTAGAACCTCCTCTATTGGGAAAATCGGTGCTGGGGGTGTTATAAGAGATCATCTTGGCAATTGGATTTCTGGTTTCCAAATTAACTTAGGCTCTGGGGAAATTTTAGATGCTGAGGCCTGGGGCTTATTCTATGGGCTCAGACTTGCTCTAAGCTGTCACATCTCTAAGCTTGAAGTGGAATCTGACTCTGCTATCCTTGTGCAGCTGATGCAGAAAGCTGATTTCTCTCTTCACCCCCTTGGTTCCCTTCTCAAGGGCTGCTCTAATATGATGATGACTATGGAGAATGCACACCTAACCCATATCTTCAGGGAATGCAATATGACGGCTGATTCTTTAGCGAAAGATAGTATTAATCACGAGCTTGGCCTTATCACTTTTGAGAATCCGCCTATCCACGTTGCTCAGGCATATCTTGATGATATTTCTGCTGTTTCTAGAGCCAGGAGAAGCTCTTGCTTTAGCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1367

Amino Acids

155.64

Weight (kDa)

8.91

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 219 1.5e-08 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 493 - 753 1.3e-28 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 1013 - 1098 4.6e-18 zinc-binding in reverse transcriptase
RVT_3 PF13456 1207 - 1327 1.3e-22 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2840, 3674
Acc36I ACCTGC 1 cut(s) 2352
AccB7I CCANNNNNTGG 1 cut(s) 2724
AccIII TCCGGA 1 cut(s) 3493
AciI CCGC 1 cut(s) 4022
AclWI GGATC 7 cut(s) 538, 2305, 2621, 2820, 2928, 3273, 3461
AcoI YGGCCR 1 cut(s) 1339
AcuI CTGAAG 3 cut(s) 636, 2841, 3925
AfaI GTAC 2 cut(s) 2722, 3316
AfiI CCNNNNNNNGG 5 cut(s) 464, 1609, 2724, 3648, 3886
AflII CTTAAG 1 cut(s) 3305
AflIII ACRYGT 1 cut(s) 3218
AhlI ACTAGT 1 cut(s) 1835
AjnI CCWGG 9 cut(s) 289, 542, 1055, 1336, 1444, 1624, 3310, 3749, 4076
AjuI GAANNNNNNNTTGG 4 cut(s) 3020, 3052, 3369, 3401
AleI CACNNNNGTG 1 cut(s) 2211
Alw21I GWGCWC 1 cut(s) 1532
Alw44I GTGCAC 1 cut(s) 1528
AlwI GGATC 7 cut(s) 538, 2305, 2621, 2820, 2928, 3273, 3461
AlwNI CAGNNNCTG 1 cut(s) 3270
Ama87I CYCGRG 1 cut(s) 135
Aor13HI TCCGGA 1 cut(s) 3493
ApaLI GTGCAC 1 cut(s) 1528
AseI ATTAAT 4 cut(s) 390, 945, 2967, 3987
Asp700I GAANNNNTTC 4 cut(s) 1001, 1401, 1687, 3249
AspS9I GGNCC 7 cut(s) 455, 912, 1578, 2015, 2503, 2640, 2938
AsuHPI GGTGA 8 cut(s) 803, 1249, 1406, 1908, 1916, 2377, 2492, 3857
AsuII TTCGAA 1 cut(s) 3062
AsuNHI GCTAGC 2 cut(s) 326, 2603
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 4 cut(s) 455, 2015, 2503, 2938
BaeGI GKGCMC 1 cut(s) 1532
BaeI ACNNNNGTAYC 2 cut(s) 2178, 2211
BalI TGGCCA 1 cut(s) 1341
BanII GRGCYC 1 cut(s) 3771
BarI GAAGNNNNNNTAC 2 cut(s) 3187, 3219
BauI CACGAG 2 cut(s) 114, 3992
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 1532
BbvCI CCTCAGC 5 cut(s) 186, 1494, 2064, 3519, 3744
BceAI ACGGC 1 cut(s) 3975
BcgI CGANNNNNNTGC 2 cut(s) 3042, 3076
BciT130I CCWGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
BciVI GTATCC 2 cut(s) 2179, 2620
BcuI ACTAGT 1 cut(s) 1835
BfrI CTTAAG 1 cut(s) 3305
BfuAI ACCTGC 1 cut(s) 2352
BfuI GTATCC 2 cut(s) 2179, 2620
BglII AGATCT 1 cut(s) 1685
BmcAI AGTACT 1 cut(s) 2722
Bme1390I CCNGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
Bme18I GGWCC 4 cut(s) 455, 2015, 2503, 2938
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 7 cut(s) 455, 912, 1578, 2015, 2503, 2640, 2938
BmiI GGNNCC 4 cut(s) 2674, 2940, 3019, 3877
BmrFI CCNGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
BmrI ACTGGG 2 cut(s) 2988, 3465
BmsI GCATC 7 cut(s) 297, 660, 1408, 1575, 2742, 3730, 3831
BmtI GCTAGC 2 cut(s) 330, 2607
BmuI ACTGGG 2 cut(s) 2988, 3465
BpiI GAAGAC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 2602, 2634
BpmI CTGGAG 3 cut(s) 565, 1027, 2629
Bpu10I CCTNAGC 7 cut(s) 186, 1494, 2064, 2598, 3519, 3744, 4038
Bpu14I TTCGAA 1 cut(s) 3062
BpuEI CTTGAG 2 cut(s) 1644, 3869
Bsa29I ATCGAT 1 cut(s) 1248
BsaBI GATNNNNATC 1 cut(s) 390
BsaI GGTCTC 2 cut(s) 971, 2223
BsaJI CCNNGG 5 cut(s) 1229, 1444, 2934, 3750, 3871
BsaWI WCCGGW 1 cut(s) 3493
Bsc4I CCNNNNNNNGG 5 cut(s) 464, 1609, 2724, 3648, 3886
Bse1I ACTGG 9 cut(s) 469, 576, 793, 1136, 2635, 2718, 2983, 3347, 3471
Bse3DI GCAATG 3 cut(s) 378, 3238, 3370
Bse8I GATNNNNATC 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 3493
BseBI CCWGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
BseCI ATCGAT 1 cut(s) 1248
BseDI CCNNGG 5 cut(s) 1229, 1444, 2934, 3750, 3871
BseGI GGATG 3 cut(s) 312, 2262, 3212
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 5 cut(s) 464, 1609, 2724, 3648, 3886
BseMI GCAATG 3 cut(s) 378, 3238, 3370
BseNI ACTGG 9 cut(s) 469, 576, 793, 1136, 2635, 2718, 2983, 3347, 3471
BseRI GAGGAG 2 cut(s) 3536, 3631
BseSI GKGCMC 1 cut(s) 1532
BseYI CCCAGC 1 cut(s) 3662
BsgI GTGCAG 1 cut(s) 3854
BshVI ATCGAT 1 cut(s) 1248
BsiHKAI GWGCWC 1 cut(s) 1532
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 1 cut(s) 3494
BslFI GGGAC 5 cut(s) 1769, 2534, 2924, 2957, 3643
BslI CCNNNNNNNGG 5 cut(s) 464, 1609, 2724, 3648, 3886
BsmFI GGGAC 5 cut(s) 1769, 2534, 2924, 2957, 3643
BsmI GAATGC 7 cut(s) 28, 508, 903, 1893, 3556, 3925, 3953
Bso31I GGTCTC 2 cut(s) 971, 2223
BsoBI CYCGRG 1 cut(s) 135
Bsp119I TTCGAA 1 cut(s) 3062
Bsp1286I GDGCHC 2 cut(s) 1532, 3771
Bsp13I TCCGGA 1 cut(s) 3493
Bsp19I CCATGG 1 cut(s) 2934
BspACI CCGC 1 cut(s) 4022
BspDI ATCGAT 1 cut(s) 1248
BspEI TCCGGA 1 cut(s) 3493
BspHI TCATGA 1 cut(s) 1414
BspLI GGNNCC 4 cut(s) 2674, 2940, 3019, 3877
BspMI ACCTGC 1 cut(s) 2352
BspOI GCTAGC 2 cut(s) 330, 2607
BspPI GGATC 7 cut(s) 538, 2305, 2621, 2820, 2928, 3273, 3461
BspQI GCTCTTC 1 cut(s) 2856
BspT104I TTCGAA 1 cut(s) 3062
BspTI CTTAAG 1 cut(s) 3305
BspTNI GGTCTC 2 cut(s) 971, 2223
BsrDI GCAATG 3 cut(s) 378, 3238, 3370
BsrI ACTGG 9 cut(s) 469, 576, 793, 1136, 2635, 2718, 2983, 3347, 3471
BssECI CCNNGG 5 cut(s) 1229, 1444, 2934, 3750, 3871
BssSI CACGAG 2 cut(s) 114, 3992
BssT1I CCWWGG 3 cut(s) 1229, 2934, 3871
Bst2BI CACGAG 2 cut(s) 114, 3992
Bst2UI CCWGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
Bst4CI ACNGT 9 cut(s) 224, 316, 2488, 2527, 2544, 3043, 3154, 3272, 3562
Bst6I CTCTTC 7 cut(s) 291, 772, 2856, 2933, 3190, 3255, 3867
BstAFI CTTAAG 1 cut(s) 3305
BstAPI GCANNNNNTGC 1 cut(s) 3841
BstBI TTCGAA 1 cut(s) 3062
BstC8I GCNNGC 7 cut(s) 296, 328, 1631, 2359, 2534, 2595, 2605
BstDSI CCRYGG 1 cut(s) 2934
BstEII GGTNACC 2 cut(s) 1255, 1904
BstENI CCTNNNNNAGG 1 cut(s) 3884
BstF5I GGATG 3 cut(s) 312, 2262, 3212
BstMWI GCNNNNNNNGC 6 cut(s) 32, 752, 1585, 1816, 2594, 3841
BstNI CCWGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
BstNSI RCATGY 1 cut(s) 3222
BstPI GGTNACC 2 cut(s) 1255, 1904
BstSCI CCNGG 9 cut(s) 289, 542, 1055, 1336, 1444, 1624, 3310, 3749, 4076
BstSLI GKGCMC 1 cut(s) 1532
BstV2I GAAGAC 1 cut(s) 597
BstX2I RGATCY 7 cut(s) 530, 1685, 2310, 2613, 2812, 2920, 3278
BstYI RGATCY 7 cut(s) 530, 1685, 2310, 2613, 2812, 2920, 3278
Bsu15I ATCGAT 1 cut(s) 1248
BsuI GTATCC 2 cut(s) 2179, 2620
BsuTUI ATCGAT 1 cut(s) 1248
BtgI CCRYGG 1 cut(s) 2934
BtsCI GGATG 3 cut(s) 312, 2262, 3212
BtsI GCAGTG 2 cut(s) 655, 1819
BtsIMutI CAGTG 7 cut(s) 312, 655, 1137, 1819, 2484, 2523, 3039
BveI ACCTGC 1 cut(s) 2352
Cac8I GCNNGC 7 cut(s) 296, 328, 1631, 2359, 2534, 2595, 2605
CaiI CAGNNNCTG 1 cut(s) 3270
CciI TCATGA 1 cut(s) 1414
Cfr13I GGNCC 7 cut(s) 455, 912, 1578, 2015, 2503, 2640, 2938
ClaI ATCGAT 1 cut(s) 1248
CsiI ACCWGGT 1 cut(s) 1055
Csp6I GTAC 2 cut(s) 2721, 3315
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviQI GTAC 2 cut(s) 2721, 3315
DraI TTTAAA 3 cut(s) 7, 1675, 2769
EaeI YGGCCR 1 cut(s) 1339
Eam1104I CTCTTC 7 cut(s) 291, 772, 2856, 2933, 3190, 3255, 3867
EarI CTCTTC 7 cut(s) 291, 772, 2856, 2933, 3190, 3255, 3867
EciI GGCGGA 1 cut(s) 4011
Eco130I CCWWGG 3 cut(s) 1229, 2934, 3871
Eco147I AGGCCT 1 cut(s) 3749
Eco24I GRGCYC 1 cut(s) 3771
Eco31I GGTCTC 2 cut(s) 971, 2223
Eco32I GATATC 1 cut(s) 1213
Eco47I GGWCC 4 cut(s) 455, 2015, 2503, 2938
Eco57I CTGAAG 3 cut(s) 636, 2841, 3925
Eco88I CYCGRG 1 cut(s) 135
Eco91I GGTNACC 2 cut(s) 1255, 1904
EcoNI CCTNNNNNAGG 1 cut(s) 3884
EcoO109I RGGNCCY 1 cut(s) 1578
EcoO65I GGTNACC 2 cut(s) 1255, 1904
EcoRI GAATTC 1 cut(s) 778
EcoRII CCWGG 9 cut(s) 289, 542, 1055, 1336, 1444, 1624, 3310, 3749, 4076
EcoRV GATATC 1 cut(s) 1213
EcoT14I CCWWGG 3 cut(s) 1229, 2934, 3871
EcoT38I GRGCYC 1 cut(s) 3771
ErhI CCWWGG 3 cut(s) 1229, 2934, 3871
FaqI GGGAC 5 cut(s) 1769, 2534, 2924, 2957, 3643
FauNDI CATATG 1 cut(s) 1500
FokI GGATG 3 cut(s) 319, 2249, 3199
FriOI GRGCYC 1 cut(s) 3771
GsaI CCCAGC 1 cut(s) 3666
GsuI CTGGAG 3 cut(s) 565, 1027, 2629
HapII CCGG 1 cut(s) 3494
HincII GTYRAC 3 cut(s) 1267, 1833, 1866
HindII GTYRAC 3 cut(s) 1267, 1833, 1866
HindIII AAGCTT 5 cut(s) 1734, 2456, 3167, 3297, 3800
HpaI GTTAAC 1 cut(s) 1267
HpaII CCGG 1 cut(s) 3494
HphI GGTGA 8 cut(s) 803, 1249, 1406, 1908, 1916, 2377, 2492, 3857
Hpy166II GTNNAC 8 cut(s) 110, 1137, 1267, 1530, 1833, 1866, 1975, 2506
Hpy8I GTNNAC 8 cut(s) 110, 1137, 1267, 1530, 1833, 1866, 1975, 2506
HpyAV CCTTC 8 cut(s) 462, 644, 1083, 1270, 1711, 2494, 2865, 3890
HpyCH4III ACNGT 9 cut(s) 224, 316, 2488, 2527, 2544, 3043, 3154, 3272, 3562
HpyCH4IV ACGT 2 cut(s) 1829, 4032
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 752, 1585, 1816, 2594, 3841
HpySE526I ACGT 2 cut(s) 1829, 4032
Kpn2I TCCGGA 1 cut(s) 3493
KspAI GTTAAC 1 cut(s) 1267
LguI GCTCTTC 1 cut(s) 2856
LmnI GCTCC 5 cut(s) 1339, 2171, 2678, 3017, 4103
LweI GCATC 7 cut(s) 297, 660, 1408, 1575, 2742, 3730, 3831
MabI ACCWGGT 1 cut(s) 1055
MaeII ACGT 2 cut(s) 1829, 4032
MaeIII GTNAC 9 cut(s) 857, 1127, 1255, 1904, 2204, 2383, 2441, 2480, 3788
MfeI CAATTG 3 cut(s) 435, 3365, 3691
MflI RGATCY 7 cut(s) 530, 1685, 2310, 2613, 2812, 2920, 3278
MhlI GDGCHC 2 cut(s) 1532, 3771
MlsI TGGCCA 1 cut(s) 1341
MluNI TGGCCA 1 cut(s) 1341
MlyI GAGTC 4 cut(s) 1313, 2283, 2912, 3812
MmeI TCCRAC 5 cut(s) 284, 652, 1272, 2312, 3332
Mox20I TGGCCA 1 cut(s) 1341
MroI TCCGGA 1 cut(s) 3493
MroXI GAANNNNTTC 4 cut(s) 1001, 1401, 1687, 3249
MscI TGGCCA 1 cut(s) 1341
MslI CAYNNNNRTG 4 cut(s) 1766, 2211, 2289, 3217
Msp20I TGGCCA 1 cut(s) 1341
MspA1I CMGCKG 3 cut(s) 2573, 2861, 3838
MspCI CTTAAG 1 cut(s) 3305
MspI CCGG 1 cut(s) 3494
MspR9I CCNGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
MunI CAATTG 3 cut(s) 435, 3365, 3691
Mva1269I GAATGC 7 cut(s) 28, 508, 903, 1893, 3556, 3925, 3953
MvaI CCWGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
MwoI GCNNNNNNNGC 6 cut(s) 32, 752, 1585, 1816, 2594, 3841
NcoI CCATGG 1 cut(s) 2934
NdeI CATATG 1 cut(s) 1500
NheI GCTAGC 2 cut(s) 326, 2603
NlaIV GGNNCC 4 cut(s) 2674, 2940, 3019, 3877
NmuCI GTSAC 8 cut(s) 857, 1255, 1904, 2204, 2383, 2441, 2480, 3788
NspI RCATGY 1 cut(s) 3222
NspV TTCGAA 1 cut(s) 3062
OliI CACNNNNGTG 1 cut(s) 2211
PaeR7I CTCGAG 1 cut(s) 135
PagI TCATGA 1 cut(s) 1414
PceI AGGCCT 1 cut(s) 3749
PciI ACATGT 1 cut(s) 3218
PciSI GCTCTTC 1 cut(s) 2856
PctI GAATGC 7 cut(s) 28, 508, 903, 1893, 3556, 3925, 3953
PdmI GAANNNNTTC 4 cut(s) 1001, 1401, 1687, 3249
PflFI GACNNNGTC 2 cut(s) 2525, 2902
PflMI CCANNNNNTGG 1 cut(s) 2724
PleI GAGTC 4 cut(s) 1312, 2282, 2911, 3812
PpsI GAGTC 4 cut(s) 1312, 2282, 2911, 3812
PscI ACATGT 1 cut(s) 3218
PshBI ATTAAT 4 cut(s) 390, 945, 2967, 3987
PsiI TTATAA 2 cut(s) 2840, 3674
Psp6I CCWGG 9 cut(s) 289, 542, 1055, 1336, 1444, 1624, 3310, 3749, 4076
PspEI GGTNACC 2 cut(s) 1255, 1904
PspFI CCCAGC 1 cut(s) 3662
PspGI CCWGG 9 cut(s) 289, 542, 1055, 1336, 1444, 1624, 3310, 3749, 4076
PspN4I GGNNCC 4 cut(s) 2674, 2940, 3019, 3877
PspPI GGNCC 7 cut(s) 455, 912, 1578, 2015, 2503, 2640, 2938
PsrI GAACNNNNNNTAC 2 cut(s) 1605, 1637
PstNI CAGNNNCTG 1 cut(s) 3270
PsuI RGATCY 7 cut(s) 530, 1685, 2310, 2613, 2812, 2920, 3278
PsyI GACNNNGTC 2 cut(s) 2525, 2902
PvuII CAGCTG 3 cut(s) 2573, 2861, 3838
RsaI GTAC 2 cut(s) 2722, 3316
RsaNI GTAC 2 cut(s) 2721, 3315
RseI CAYNNNNRTG 4 cut(s) 1766, 2211, 2289, 3217
SapI GCTCTTC 1 cut(s) 2856
Sau96I GGNCC 7 cut(s) 455, 912, 1578, 2015, 2503, 2640, 2938
ScaI AGTACT 1 cut(s) 2722
SchI GAGTC 4 cut(s) 1313, 2283, 2912, 3812
ScrFI CCNGG 9 cut(s) 291, 544, 1057, 1338, 1446, 1626, 3312, 3751, 4078
SduI GDGCHC 2 cut(s) 1532, 3771
SexAI ACCWGGT 1 cut(s) 1055
SfaNI GCATC 7 cut(s) 297, 660, 1408, 1575, 2742, 3730, 3831
Sfr274I CTCGAG 1 cut(s) 135
SfuI TTCGAA 1 cut(s) 3062
SinI GGWCC 4 cut(s) 455, 2015, 2503, 2938
SlaI CTCGAG 1 cut(s) 135
SmiMI CAYNNNNRTG 4 cut(s) 1766, 2211, 2289, 3217
SmlI CTYRAG 4 cut(s) 135, 1659, 3305, 3884
SmoI CTYRAG 4 cut(s) 135, 1659, 3305, 3884
SpeI ACTAGT 1 cut(s) 1835
SseBI AGGCCT 1 cut(s) 3749
SsiI CCGC 1 cut(s) 4022
SspI AATATT 2 cut(s) 2246, 2680
StuI AGGCCT 1 cut(s) 3749
StyD4I CCNGG 9 cut(s) 289, 542, 1055, 1336, 1444, 1624, 3310, 3749, 4076
StyI CCWWGG 3 cut(s) 1229, 2934, 3871
TaaI ACNGT 9 cut(s) 224, 316, 2488, 2527, 2544, 3043, 3154, 3272, 3562
TaiI ACGT 2 cut(s) 1832, 4035
TaqI TCGA 5 cut(s) 52, 136, 1248, 2196, 3062
TatI WGTACW 1 cut(s) 2720
TscAI CASTG 7 cut(s) 319, 662, 1144, 1826, 2491, 2530, 3046
TseFI GTSAC 8 cut(s) 857, 1255, 1904, 2204, 2383, 2441, 2480, 3788
Tsp45I GTSAC 8 cut(s) 857, 1255, 1904, 2204, 2383, 2441, 2480, 3788
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 7 cut(s) 319, 662, 1144, 1826, 2491, 2530, 3046
Tth111I GACNNNGTC 2 cut(s) 2525, 2902
Van91I CCANNNNNTGG 1 cut(s) 2724
Vha464I CTTAAG 1 cut(s) 3305
VneI GTGCAC 1 cut(s) 1528
VpaK11BI GGWCC 4 cut(s) 455, 2015, 2503, 2938
VspI ATTAAT 4 cut(s) 390, 945, 2967, 3987
XagI CCTNNNNNAGG 1 cut(s) 3884
XbaI TCTAGA 1 cut(s) 4070
XceI RCATGY 1 cut(s) 3222
XhoI CTCGAG 1 cut(s) 135
XmnI GAANNNNTTC 4 cut(s) 1001, 1401, 1687, 3249
ZrmI AGTACT 1 cut(s) 2722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.