pycom10g14880

Monothiol glutaredoxin-S12, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013412
Physical Location & Seq
Reverse (-)
18238456 .. 18241767
3312 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 726 bp
ATGCTCTGCCTCTGTGGCTTGTGTTCTCCTCAGTCCAACTGCCAAAAGCCTCAAACCAACGGCAGCGGAGGAAGCAAGCAAAGTGAGATAGGCCAAAAAGCCAAGCAAAAAGCTCCGAGAAAGCCGGAAGCCACCGACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACAGCAACAACAGCAATGGCAGTCTCATATAATCTCACCAATTTCGCATCCGTTTCTCTAAATTCCCCTCGCATTCTTTCGTCTGCAAACAAGTCATCCAGTTTCTGTGTTCCTTTCAACAACAGTTACAGTTCGGGAGGTAGCTCGAGCACCAACCTTTCCAGCGTCCATGGCGGCTCGAGACGATGGAGACCCATGTCGGTTCAGGCCATGGCGTCCTCCTCCTTCGGTTCCCGGCTCGAAGAGAGCGTTAAGAAGACGGTGGATGAGAACCCTGTTGTCGTCTATTCCAAAACTTGGTGTTCGTACTCATCTGAGGTGAAATCGTTATTCAAAAGGCTTGGTGTGGAGCCGATCGTGATTGAATTGGATGAACTGGGTCCCCAAGGACCACAGCTGCAAAAGGTATTGGAGAGGCTTACAGGACAACATACTGTCCCTAATGTTTTTATCGGTGGGAAACACATTGGGGGTTGTACAGATACTGTGAAGTTATACCGAAAAGGTGAACTTGAAACTTTGCTCTCAGAAGCGAGTGCTAAAAATACACAAAGCTAA

Protein Analysis

242

Amino Acids

26.16

Weight (kDa)

9.27

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 149 - 212 1.5e-16 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28730 AT4G28730
fragaria_vesca FvH4_2g20100
malus_domestica MD05G1184700.v1.1 MD10G1172500.v1.1
prunus_persica Prupe.8G210900_v2.0.a1
pyrus_communis pycom05g16950 pycom10g14880
rosa_chinensis RchiOBHm_Chr6g0285841
rosa_laevigata RLG00000012605
rosa_multiflora Rmu_sc0004321.1_g000007
rosa_roxburghii Rroxscaffold_7G00182660
rosa_rugosa Rorug06G0177200
rosa_samantha Rh6AG289000 Rh6BG291000 Rh6CG291900 Rh6DG283900
rosa_wichuraiana Rw6G024900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 465
AciI CCGC 2 cut(s) 66, 342
AcsI RAATTY 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 2 cut(s) 476, 646
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 4 cut(s) 286, 502, 533, 683
AluBI AGCT 4 cut(s) 113, 312, 565, 723
AluI AGCT 4 cut(s) 113, 312, 565, 723
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 4 cut(s) 161, 196, 343, 352
AlwNI CAGNNNCTG 2 cut(s) 273, 653
Ama87I CYCGRG 2 cut(s) 313, 346
AoxI GGCC 2 cut(s) 91, 375
ApeKI GCWGC 2 cut(s) 63, 565
ApoI RAATTY 1 cut(s) 229
AspS9I GGNCC 2 cut(s) 548, 557
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 3 cut(s) 196, 499, 686
AvaI CYCGRG 2 cut(s) 313, 346
AvaII GGWCC 2 cut(s) 548, 557
BbsI GAAGAC 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 2 cut(s) 75, 552
BccI CCATC 1 cut(s) 348
BceAI ACGGC 1 cut(s) 76
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 4 cut(s) 161, 196, 343, 352
BglI GCCNNNNNGGC 1 cut(s) 15
BisI GCNGC 3 cut(s) 64, 343, 566
BlsI GCNGC 3 cut(s) 65, 344, 567
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 2 cut(s) 548, 557
BmeT110I CYCGRG 2 cut(s) 313, 346
BmgT120I GGNCC 2 cut(s) 548, 557
BmiI GGNNCC 4 cut(s) 400, 519, 549, 550
BmrFI CCNGG 1 cut(s) 403
BmrI ACTGGG 1 cut(s) 554
BmsI GCATC 1 cut(s) 224
BmuI ACTGGG 1 cut(s) 554
BoxI GACNNNNGTC 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 383
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 3 cut(s) 337, 378, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 465
Bse1I ACTGG 2 cut(s) 267, 549
Bse3DI GCAATG 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 337, 378, 553
BseGI GGATG 4 cut(s) 215, 263, 439, 544
BseLI CCNNNNNNNGG 1 cut(s) 465
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 3 cut(s) 44, 474, 708
BseNI ACTGG 2 cut(s) 267, 549
BseRI GAGGAG 2 cut(s) 18, 379
BseXI GCAGC 2 cut(s) 75, 552
Bsh1285I CGRYCG 1 cut(s) 525
BshFI GGCC 2 cut(s) 93, 377
BsiEI CGRYCG 1 cut(s) 525
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 2 cut(s) 313, 346
BsiSI CCGG 2 cut(s) 125, 403
BslFI GGGAC 2 cut(s) 534, 590
BslI CCNNNNNNNGG 1 cut(s) 465
BsmAI GTCTC 4 cut(s) 161, 196, 343, 352
BsmBI CGTCTC 1 cut(s) 343
BsmFI GGGAC 2 cut(s) 534, 590
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 2 cut(s) 93, 377
Bso31I GGTCTC 1 cut(s) 352
BsoBI CYCGRG 2 cut(s) 313, 346
Bsp1286I GDGCHC 1 cut(s) 320
Bsp1407I TGTACA 1 cut(s) 644
Bsp143I GATC 1 cut(s) 522
Bsp19I CCATGG 2 cut(s) 337, 378
BspACI CCGC 2 cut(s) 66, 342
BspANI GGCC 2 cut(s) 93, 377
BspCNI CTCAG 3 cut(s) 43, 475, 707
BspLI GGNNCC 4 cut(s) 400, 519, 549, 550
BspTNI GGTCTC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 189
BsrGI TGTACA 1 cut(s) 644
BsrI ACTGG 2 cut(s) 267, 549
BssECI CCNNGG 3 cut(s) 337, 378, 553
BssMI GATC 1 cut(s) 522
BssNI GRCGYC 1 cut(s) 383
BssT1I CCWWGG 3 cut(s) 337, 378, 553
Bst4CI ACNGT 5 cut(s) 293, 299, 430, 604, 655
Bst6I CTCTTC 1 cut(s) 405
BstACI GRCGYC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 644
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 3 cut(s) 30, 483, 694
BstDSI CCRYGG 2 cut(s) 337, 378
BstF5I GGATG 4 cut(s) 215, 263, 439, 544
BstKTI GATC 1 cut(s) 525
BstMAI GTCTC 4 cut(s) 161, 196, 343, 352
BstMBI GATC 1 cut(s) 522
BstMCI CGRYCG 1 cut(s) 525
BstMWI GCNNNNNNNGC 4 cut(s) 15, 72, 179, 339
BstPAI GACNNNNGTC 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 401
BstV1I GCAGC 2 cut(s) 75, 552
BstV2I GAAGAC 1 cut(s) 431
BsuRI GGCC 2 cut(s) 93, 377
BtgI CCRYGG 2 cut(s) 337, 378
BtsCI GGATG 4 cut(s) 215, 263, 439, 544
Cac8I GCNNGC 1 cut(s) 77
CaiI CAGNNNCTG 2 cut(s) 273, 653
Cfr13I GGNCC 2 cut(s) 548, 557
CseI GACGC 2 cut(s) 322, 372
Csp6I GTAC 2 cut(s) 475, 645
CviAII CATG 3 cut(s) 338, 364, 379
CviQI GTAC 2 cut(s) 475, 645
DdeI CTNAG 3 cut(s) 30, 483, 694
DpnI GATC 1 cut(s) 524
DpnII GATC 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco130I CCWWGG 3 cut(s) 337, 378, 553
Eco31I GGTCTC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 548, 557
Eco88I CYCGRG 2 cut(s) 313, 346
EcoO109I RGGNCCY 1 cut(s) 548
EcoT14I CCWWGG 3 cut(s) 337, 378, 553
ErhI CCWWGG 3 cut(s) 337, 378, 553
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 3 cut(s) 341, 367, 382
FaiI YATR 7 cut(s) 196, 198, 339, 365, 380, 600, 664
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FaqI GGGAC 2 cut(s) 534, 590
FatI CATG 3 cut(s) 337, 363, 378
Fnu4HI GCNGC 3 cut(s) 64, 343, 566
FokI GGATG 4 cut(s) 202, 250, 446, 551
Fsp4HI GCNGC 3 cut(s) 64, 343, 566
GluI GCNGC 3 cut(s) 64, 343, 566
HaeIII GGCC 2 cut(s) 93, 377
HapII CCGG 2 cut(s) 125, 403
HgaI GACGC 2 cut(s) 322, 372
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 3 cut(s) 341, 367, 382
HpaII CCGG 2 cut(s) 125, 403
HphI GGTGA 3 cut(s) 196, 499, 686
Hpy166II GTNNAC 1 cut(s) 677
Hpy188I TCNGA 3 cut(s) 117, 484, 697
Hpy188III TCNNGA 3 cut(s) 303, 348, 526
Hpy8I GTNNAC 1 cut(s) 677
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 5 cut(s) 293, 299, 430, 604, 655
HpyCH4V TGCA 2 cut(s) 254, 568
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 72, 179, 339
HpyF3I CTNAG 3 cut(s) 30, 483, 694
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 3 cut(s) 341, 367, 382
KflI GGGWCCC 1 cut(s) 548
Kzo9I GATC 1 cut(s) 522
LmnI GCTCC 2 cut(s) 118, 517
LpnPI CCDG 8 cut(s) 138, 280, 343, 359, 416, 456, 530, 576
Lsp1109I GCAGC 2 cut(s) 75, 552
LweI GCATC 1 cut(s) 224
MaeIII GTNAC 1 cut(s) 293
MalI GATC 1 cut(s) 524
MboI GATC 1 cut(s) 522
MboII GAAGA 2 cut(s) 422, 436
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 3 cut(s) 208, 229, 533
MmeI TCCRAC 1 cut(s) 60
MseI TTAA 1 cut(s) 420
MspA1I CMGCKG 2 cut(s) 66, 565
MspI CCGG 2 cut(s) 125, 403
MspR9I CCNGG 1 cut(s) 403
Mva1269I GAATGC 1 cut(s) 240
MwoI GCNNNNNNNGC 4 cut(s) 15, 72, 179, 339
NciI CCSGG 1 cut(s) 403
NcoI CCATGG 2 cut(s) 337, 378
NdeII GATC 1 cut(s) 522
NlaIII CATG 3 cut(s) 341, 367, 382
NlaIV GGNNCC 4 cut(s) 400, 519, 549, 550
PaeR7I CTCGAG 2 cut(s) 313, 346
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PctI GAATGC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 465
PkrI GCNGC 3 cut(s) 65, 344, 567
Ple19I CGATCG 1 cut(s) 525
PpuMI RGGWCCY 1 cut(s) 548
PshAI GACNNNNGTC 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 548
PspN4I GGNNCC 4 cut(s) 400, 519, 549, 550
PspPI GGNCC 2 cut(s) 548, 557
PspPPI RGGWCCY 1 cut(s) 548
PspXI VCTCGAGB 1 cut(s) 313
PstNI CAGNNNCTG 2 cut(s) 273, 653
PvuI CGATCG 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 565
RsaI GTAC 2 cut(s) 476, 646
RsaNI GTAC 2 cut(s) 475, 645
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 3 cut(s) 64, 343, 566
Sau3AI GATC 1 cut(s) 522
Sau96I GGNCC 2 cut(s) 548, 557
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 1 cut(s) 320
SetI ASST 9 cut(s) 115, 310, 314, 327, 489, 567, 576, 676, 725
SfaNI GCATC 1 cut(s) 224
Sfr274I CTCGAG 2 cut(s) 313, 346
SinI GGWCC 2 cut(s) 548, 557
SlaI CTCGAG 2 cut(s) 313, 346
SmlI CTYRAG 2 cut(s) 313, 346
SmoI CTYRAG 2 cut(s) 313, 346
Sse9I AATT 3 cut(s) 208, 229, 533
SsiI CCGC 2 cut(s) 66, 342
StyD4I CCNGG 1 cut(s) 401
StyI CCWWGG 3 cut(s) 337, 378, 553
TaaI ACNGT 5 cut(s) 293, 299, 430, 604, 655
TaqI TCGA 3 cut(s) 314, 347, 408
TasI AATT 3 cut(s) 208, 229, 533
TatI WGTACW 1 cut(s) 644
TauI GCSGC 1 cut(s) 345
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TseI GCWGC 2 cut(s) 63, 565
TspDTI ATGAA 1 cut(s) 555
TspGWI ACGGA 1 cut(s) 208
Van91I CCANNNNNTGG 1 cut(s) 465
VpaK11BI GGWCC 2 cut(s) 548, 557
XapI RAATTY 1 cut(s) 229
XhoI CTCGAG 2 cut(s) 313, 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.