pycom10g16520

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017246
Physical Location & Seq
Forward (+)
19727125 .. 19729272
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGTCACTTCCACCACCGGATCCAACAGCGGAAGCACCAAAAGATGAACAAGTGGAGGTGGATCACGAAAGGCAAGTAGCACAAGAAGAAGAAGAAGCAAAACATGGTCAAGGCACGTGGAAGCACGCCGCTTTCCACGTGGCCACCACGATTGCCACCCCTGCCGCCTATGCACCCCTGCCTTTCGCCTTGGCCTCTCTCGGCTGGCCTCTTGGGGTGAGCAGTTTGGTGGGAGCAACACTAGCCACGTGGTATTCAAGCTTTCTCGTTGCTTCTCTGTGGAAATGGGATGGGAAGAAACACGTCACCTATCGCCACCTGGCAAAAAGCATTTTTGGGTTCTGGGGTTATTGGTCAATTGCATTTTTTCAACAGTTGGCCTCTATAGGCAATAATATAGCCATTCAGATTGCAGCTGGAAGCAGCATTAAGGCTGTATACAAATATTATCACAGTGAAGGTAGCTTAACCCTGCAGCACTTCATCATTTTCTTTGGGGCATTTGAGCTCTTGCTTTCTCAGCTGCCAGATATCCACTCACTGAGATGGGTAAATGCCTTGTGCACTTTCAGCACCATTGGCTTTGCTGCCACAACAATTGGTGTCACAATTTACAACGGGAAAAAGATTGACAGAAAATCAGTGAGTTATAGTTTGGATGGAAGCTCATCTTCTAAGGCCTTCAGAGCCTTTAACGCTCTTGGCGCAATTGCCTTTTCCTTTGGAGATGCAATGCTTCCGGAGATCCAGAATACAGTGAGAGAACCTGCAAAGAAGAACATGTATAAAGGTGTATCAGCAGCATATGGTGTCATTGTGTTGAGTTACTGGCAGTTGGCCTTCACTGGGTACTGGGCTTTTGGCTCACATGTGCAACCTTACATCTTGTCTTCTCTAACTGTTCCCCAATGGACCATTGTCATGGCCAATCTTTTTGCTGTCATACAAATTTCAGGATGTTTTCAGATATATTGCAGGCCAACATATGCCTACTTTGAGGAGGGACTCCTGTCGCACAAAACCACCAGCACCAGTCATTTCTGGCTCCGAAAAACCCTAATTCGGCTTGTTTTCACCTCGATTTATATGGTTCTGATCACTCTTGTTGCAGCAGCCGTGCCTTTCTTTGGGGACTTTGTGTCAATCTGTGGAGCAATTGGGTTCACACCATTGGACTTTGTGTTCCCTGCGTTGGCATATTTAAAAGCTAGAAGAAGAAGGCAGACCCAAAACACCAAAATGTACCTTTCTTTGCTGCTTCTCAACTTTGCATTGGTTACTTGGTTCTCTGTTGTGGCAGTTTTGGGCTGCGTTGGTGCTGTCAAGTTTATTGTGGAAGATGTCAAGACTTACCAATTCTTTCATGATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

458

Amino Acids

50.85

Weight (kDa)

9.05

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 37 - 434 5.6e-74 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22320
malus_domestica MD10G1191600.v1.1
prunus_persica Prupe.8G230900_v2.0.a1
pyrus_communis pycom10g16520
rosa_chinensis RchiOBHm_Chr6g0289321
rosa_laevigata RLG00000012313
rosa_multiflora Rmu_sc0001527.1_g000025 Rmu_sc0005115.1_g000002
rosa_roxburghii Rroxscaffold_7G00179340
rosa_rugosa Rorug06G0202800 Rorug06G0203000
rosa_samantha Rh6AG313600
rosa_wichuraiana Rw6G027110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 775
AccI GTMKAC 1 cut(s) 438
AccIII TCCGGA 1 cut(s) 739
AciI CCGC 3 cut(s) 29, 129, 165
AclWI GGATC 4 cut(s) 14, 27, 69, 739
AcoI YGGCCR 2 cut(s) 141, 924
AcsI RAATTY 1 cut(s) 948
AcuI CTGAAG 1 cut(s) 667
AcvI CACGTG 3 cut(s) 117, 139, 249
AfaI GTAC 2 cut(s) 851, 1244
AfiI CCNNNNNNNGG 4 cut(s) 846, 1062, 1127, 1192
AflIII ACRYGT 3 cut(s) 301, 780, 868
AgsI TTSAA 2 cut(s) 258, 371
AhdI GACNNNNNGTC 1 cut(s) 1138
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 318
AloI GAACNNNNNNTCC 2 cut(s) 1166, 1198
AluBI AGCT 7 cut(s) 261, 416, 465, 508, 523, 666, 1208
AluI AGCT 7 cut(s) 261, 416, 465, 508, 523, 666, 1208
Alw21I GWGCWC 2 cut(s) 510, 566
Alw44I GTGCAC 1 cut(s) 562
AlwI GGATC 4 cut(s) 14, 27, 69, 739
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 8 cut(s) 141, 192, 206, 378, 678, 837, 924, 977
ApaLI GTGCAC 1 cut(s) 562
ApoI RAATTY 1 cut(s) 948
AspLEI GCGC 1 cut(s) 707
AspS9I GGNCC 1 cut(s) 912
AsuHPI GGTGA 3 cut(s) 229, 298, 1066
AvaII GGWCC 1 cut(s) 912
BaeGI GKGCMC 1 cut(s) 566
BalI TGGCCA 2 cut(s) 143, 926
BamHI GGATCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 510
BbrPI CACGTG 3 cut(s) 117, 139, 249
BbsI GAAGAC 1 cut(s) 882
Bbv12I GWGCWC 2 cut(s) 510, 566
BccI CCATC 3 cut(s) 284, 540, 653
BceAI ACGGC 1 cut(s) 1100
BciT130I CCWGG 1 cut(s) 320
BclI TGATCA 1 cut(s) 1095
BfaI CTAG 2 cut(s) 242, 1209
BfmI CTRYAG 2 cut(s) 384, 473
BfuAI ACCTGC 1 cut(s) 775
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 912
BmeRI GACNNNNNGTC 1 cut(s) 1138
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 912
BmiI GGNNCC 2 cut(s) 21, 1046
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 2 cut(s) 855, 862
BmsI GCATC 1 cut(s) 718
BmuI ACTGGG 2 cut(s) 855, 862
BoxI GACNNNNGTC 2 cut(s) 917, 1009
BpiI GAAGAC 1 cut(s) 882
BsaAI YACGTR 3 cut(s) 117, 139, 249
BsaJI CCNNGG 1 cut(s) 189
BsaWI WCCGGW 2 cut(s) 16, 739
Bsc4I CCNNNNNNNGG 4 cut(s) 846, 1062, 1127, 1192
Bse1I ACTGG 4 cut(s) 833, 850, 857, 1032
Bse3DI GCAATG 1 cut(s) 738
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 3 cut(s) 295, 664, 962
BseLI CCNNNNNNNGG 4 cut(s) 846, 1062, 1127, 1192
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 2 cut(s) 533, 533
BseNI ACTGG 4 cut(s) 833, 850, 857, 1032
BseRI GAGGAG 1 cut(s) 1013
BseSI GKGCMC 1 cut(s) 566
BshFI GGCC 8 cut(s) 143, 194, 208, 380, 680, 839, 926, 979
BsiHKAI GWGCWC 2 cut(s) 510, 566
BsiSI CCGG 2 cut(s) 17, 740
BslFI GGGAC 2 cut(s) 1017, 1145
BslI CCNNNNNNNGG 4 cut(s) 846, 1062, 1127, 1192
BsmFI GGGAC 2 cut(s) 1017, 1145
BsnI GGCC 8 cut(s) 143, 194, 208, 380, 680, 839, 926, 979
Bsp1286I GDGCHC 2 cut(s) 510, 566
Bsp13I TCCGGA 1 cut(s) 739
Bsp143I GATC 4 cut(s) 19, 61, 744, 1095
BspACI CCGC 3 cut(s) 29, 129, 165
BspANI GGCC 8 cut(s) 143, 194, 208, 380, 680, 839, 926, 979
BspCNI CTCAG 2 cut(s) 532, 534
BspEI TCCGGA 1 cut(s) 739
BspHI TCATGA 1 cut(s) 1363
BspLI GGNNCC 2 cut(s) 21, 1046
BspMAI CTGCAG 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 775
BspPI GGATC 4 cut(s) 14, 27, 69, 739
BsrDI GCAATG 1 cut(s) 738
BsrI ACTGG 4 cut(s) 833, 850, 857, 1032
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 4 cut(s) 19, 61, 744, 1095
BssNAI GTATAC 1 cut(s) 439
BssT1I CCWWGG 1 cut(s) 189
Bst1107I GTATAC 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 4 cut(s) 375, 455, 757, 901
BstBAI YACGTR 3 cut(s) 117, 139, 249
BstC8I GCNNGC 3 cut(s) 126, 206, 977
BstDEI CTNAG 3 cut(s) 519, 542, 675
BstF5I GGATG 3 cut(s) 295, 664, 962
BstHHI GCGC 1 cut(s) 707
BstKTI GATC 4 cut(s) 22, 64, 747, 1098
BstMBI GATC 4 cut(s) 19, 61, 744, 1095
BstMWI GCNNNNNNNGC 9 cut(s) 161, 170, 242, 520, 570, 579, 686, 695, 704
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 2 cut(s) 784, 872
BstPAI GACNNNNGTC 2 cut(s) 917, 1009
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 2 cut(s) 384, 473
BstSLI GKGCMC 1 cut(s) 566
BstV2I GAAGAC 1 cut(s) 882
BstX2I RGATCY 2 cut(s) 19, 744
BstXI CCANNNNNNTGG 1 cut(s) 922
BstYI RGATCY 2 cut(s) 19, 744
BstZ17I GTATAC 1 cut(s) 439
BsuRI GGCC 8 cut(s) 143, 194, 208, 380, 680, 839, 926, 979
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 3 cut(s) 295, 664, 962
BtsIMutI CAGTG 5 cut(s) 460, 539, 648, 762, 843
BveI ACCTGC 1 cut(s) 775
Cac8I GCNNGC 3 cut(s) 126, 206, 977
CciI TCATGA 1 cut(s) 1363
CfoI GCGC 1 cut(s) 707
Cfr13I GGNCC 1 cut(s) 912
Csp6I GTAC 2 cut(s) 850, 1243
CspCI CAANNNNNGTGG 6 cut(s) 98, 133, 133, 168, 580, 615
CviAII CATG 5 cut(s) 104, 781, 869, 922, 1364
CviQI GTAC 2 cut(s) 850, 1243
DdeI CTNAG 3 cut(s) 519, 542, 675
DpnI GATC 4 cut(s) 21, 63, 746, 1097
DpnII GATC 4 cut(s) 19, 61, 744, 1095
DraI TTTAAA 1 cut(s) 1203
DriI GACNNNNNGTC 1 cut(s) 1138
EaeI YGGCCR 2 cut(s) 141, 924
Eam1105I GACNNNNNGTC 1 cut(s) 1138
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 680
Eco24I GRGCYC 1 cut(s) 510
Eco32I GATATC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 912
Eco53kI GAGCTC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 667
Eco72I CACGTG 3 cut(s) 117, 139, 249
EcoICRI GAGCTC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 318
EcoRV GATATC 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 5 cut(s) 107, 784, 872, 925, 1367
FalI AAGNNNNNCTT 2 cut(s) 655, 687
FaqI GGGAC 2 cut(s) 1017, 1145
FatI CATG 5 cut(s) 103, 780, 868, 921, 1363
FauNDI CATATG 2 cut(s) 805, 985
FbaI TGATCA 1 cut(s) 1095
FblI GTMKAC 1 cut(s) 438
FokI GGATG 3 cut(s) 302, 671, 969
FriOI GRGCYC 1 cut(s) 510
FspBI CTAG 2 cut(s) 242, 1209
GlaI GCGC 1 cut(s) 706
HaeIII GGCC 8 cut(s) 143, 194, 208, 380, 680, 839, 926, 979
HapII CCGG 2 cut(s) 17, 740
HhaI GCGC 1 cut(s) 707
Hin1II CATG 5 cut(s) 107, 784, 872, 925, 1367
Hin6I GCGC 1 cut(s) 705
HinP1I GCGC 1 cut(s) 705
HindIII AAGCTT 1 cut(s) 259
HinfI GANTC 1 cut(s) 1005
HpaII CCGG 2 cut(s) 17, 740
HphI GGTGA 3 cut(s) 229, 298, 1066
Hpy166II GTNNAC 3 cut(s) 439, 564, 1164
Hpy188I TCNGA 5 cut(s) 408, 686, 966, 1049, 1095
Hpy188III TCNNGA 6 cut(s) 65, 740, 748, 954, 1345, 1364
Hpy8I GTNNAC 3 cut(s) 439, 564, 1164
HpyAV CCTTC 4 cut(s) 452, 691, 850, 1212
HpyCH4III ACNGT 4 cut(s) 375, 455, 757, 901
HpyCH4IV ACGT 4 cut(s) 116, 138, 248, 303
HpyF10VI GCNNNNNNNGC 9 cut(s) 161, 170, 242, 520, 570, 579, 686, 695, 704
HpyF3I CTNAG 3 cut(s) 519, 542, 675
HpySE526I ACGT 4 cut(s) 116, 138, 248, 303
Hsp92II CATG 5 cut(s) 107, 784, 872, 925, 1367
HspAI GCGC 1 cut(s) 705
Kpn2I TCCGGA 1 cut(s) 739
Ksp22I TGATCA 1 cut(s) 1095
Kzo9I GATC 4 cut(s) 19, 61, 744, 1095
LmnI GCTCC 3 cut(s) 233, 1050, 1151
LweI GCATC 1 cut(s) 718
MaeI CTAG 2 cut(s) 242, 1209
MaeII ACGT 4 cut(s) 116, 138, 248, 303
MaeIII GTNAC 5 cut(s) 3, 304, 604, 824, 1276
MalI GATC 4 cut(s) 21, 63, 746, 1097
MboI GATC 4 cut(s) 19, 61, 744, 1095
MfeI CAATTG 4 cut(s) 357, 597, 708, 1155
MflI RGATCY 2 cut(s) 19, 744
MhlI GDGCHC 2 cut(s) 510, 566
MlsI TGGCCA 2 cut(s) 143, 926
MluCI AATT 8 cut(s) 357, 597, 609, 708, 948, 1059, 1155, 1355
MluNI TGGCCA 2 cut(s) 143, 926
MlyI GAGTC 1 cut(s) 999
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 7 cut(s) 49, 205, 219, 391, 991, 994, 1087
Mox20I TGGCCA 2 cut(s) 143, 926
MroI TCCGGA 1 cut(s) 739
MscI TGGCCA 2 cut(s) 143, 926
MseI TTAA 4 cut(s) 429, 467, 693, 1202
MslI CAYNNNNRTG 3 cut(s) 544, 920, 1238
Msp20I TGGCCA 2 cut(s) 143, 926
MspA1I CMGCKG 3 cut(s) 29, 416, 523
MspI CCGG 2 cut(s) 17, 740
MspR9I CCNGG 1 cut(s) 320
MunI CAATTG 4 cut(s) 357, 597, 708, 1155
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 9 cut(s) 161, 170, 242, 520, 570, 579, 686, 695, 704
NdeI CATATG 2 cut(s) 805, 985
NdeII GATC 4 cut(s) 19, 61, 744, 1095
NlaIII CATG 5 cut(s) 107, 784, 872, 925, 1367
NlaIV GGNNCC 2 cut(s) 21, 1046
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 3 cut(s) 3, 304, 604
NspI RCATGY 2 cut(s) 784, 872
PagI TCATGA 1 cut(s) 1363
PceI AGGCCT 1 cut(s) 680
PciI ACATGT 2 cut(s) 780, 868
PleI GAGTC 1 cut(s) 999
PmaCI CACGTG 3 cut(s) 117, 139, 249
PmlI CACGTG 3 cut(s) 117, 139, 249
PpsI GAGTC 1 cut(s) 999
Ppu21I YACGTR 3 cut(s) 117, 139, 249
PscI ACATGT 2 cut(s) 780, 868
PshAI GACNNNNGTC 2 cut(s) 917, 1009
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 318
PspCI CACGTG 3 cut(s) 117, 139, 249
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 21, 1046
PspPI GGNCC 1 cut(s) 912
PstI CTGCAG 1 cut(s) 477
PsuI RGATCY 2 cut(s) 19, 744
PvuII CAGCTG 2 cut(s) 416, 523
RsaI GTAC 2 cut(s) 851, 1244
RsaNI GTAC 2 cut(s) 850, 1243
RseI CAYNNNNRTG 3 cut(s) 544, 920, 1238
SacI GAGCTC 1 cut(s) 510
SaqAI TTAA 4 cut(s) 429, 467, 693, 1202
Sau3AI GATC 4 cut(s) 19, 61, 744, 1095
Sau96I GGNCC 1 cut(s) 912
SchI GAGTC 1 cut(s) 999
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 2 cut(s) 510, 566
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 2 cut(s) 384, 473
SinI GGWCC 1 cut(s) 912
SmiMI CAYNNNNRTG 3 cut(s) 544, 920, 1238
Sse9I AATT 8 cut(s) 357, 597, 609, 708, 948, 1059, 1155, 1355
SseBI AGGCCT 1 cut(s) 680
SsiI CCGC 3 cut(s) 29, 129, 165
SspI AATATT 1 cut(s) 446
SspMI CTAG 2 cut(s) 242, 1209
SstI GAGCTC 1 cut(s) 510
StuI AGGCCT 1 cut(s) 680
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 4 cut(s) 375, 455, 757, 901
TaiI ACGT 4 cut(s) 119, 141, 251, 306
TaqI TCGA 1 cut(s) 1079
TasI AATT 8 cut(s) 357, 597, 609, 708, 948, 1059, 1155, 1355
TauI GCSGC 2 cut(s) 131, 167
Tru1I TTAA 4 cut(s) 429, 467, 693, 1202
Tru9I TTAA 4 cut(s) 429, 467, 693, 1202
TscAI CASTG 5 cut(s) 460, 546, 648, 762, 850
TseFI GTSAC 3 cut(s) 3, 304, 604
Tsp45I GTSAC 3 cut(s) 3, 304, 604
TspDTI ATGAA 3 cut(s) 60, 472, 1352
TspRI CASTG 5 cut(s) 460, 546, 648, 762, 850
VneI GTGCAC 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 912
XapI RAATTY 1 cut(s) 948
XceI RCATGY 2 cut(s) 784, 872
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 2 cut(s) 242, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.