pycom10g17420

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008547
Physical Location & Seq
Forward (+)
20654563 .. 20654961
399 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g17420.1

Sequence Viewer

Length: 399 bp
ATGGCTGCTGGACCGACCATACTCGAAACAGTCCAAGAGTTAGCAAGAGAAAATGGTTCTTCCCAGGTGAAAACTCAAGTGCTAAATGGCCCTGCTGATGCCTTGAAAAGGCGGTTGCAGCAGCTGATTGACTCCAATCCAGTCATGCTTTTCATGAAAGGTAGCCCTGAGGCGCCCCAATGTGGATTCAGCCAAAAAGTTGTTGACATTTTGAAGGAAGAGAATGTCAAATATGGAAGTTTCGATATTCTTTCGGACAGTGAAGTCAGGGAGGGGTTGAAGAAGTATTCTAACTGGCCAACGTTTCCGCAGCTTTACTGCAAAGGGGAGCTTCTTGGTGGGAGCTGGACGTCGCAAAAAAAAAAAACAAGAACTCTGACTCACACTTTCGCCACATGA

Protein Analysis

133

Amino Acids

14.68

Weight (kDa)

8.91

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 48 - 111 1.4e-20 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AatII GACGTC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 172
AciI CCGC 2 cut(s) 112, 308
AclI AACGTT 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 296
AcyI GRCGYC 2 cut(s) 173, 350
AfiI CCNNNNNNNGG 2 cut(s) 108, 182
AgsI TTSAA 3 cut(s) 106, 214, 280
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 4 cut(s) 124, 313, 331, 345
AluI AGCT 4 cut(s) 124, 313, 331, 345
AlwNI CAGNNNCTG 1 cut(s) 124
AoxI GGCC 2 cut(s) 88, 296
ApeKI GCWGC 4 cut(s) 5, 118, 121, 310
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 2 cut(s) 11, 89
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 11
AxyI CCTNAGG 1 cut(s) 168
BalI TGGCCA 1 cut(s) 298
BanI GGYRCC 1 cut(s) 172
BbvI GCAGC 3 cut(s) 130, 133, 322
BciT130I CCWGG 1 cut(s) 65
BfoI RGCGCY 1 cut(s) 176
BisI GCNGC 4 cut(s) 6, 119, 122, 311
BlsI GCNGC 4 cut(s) 7, 120, 123, 312
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 2 cut(s) 11, 89
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 60
BsaHI GRCGYC 2 cut(s) 173, 350
BsaJI CCNNGG 1 cut(s) 63
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 182
Bse1I ACTGG 2 cut(s) 140, 299
Bse21I CCTNAGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 108, 182
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 2 cut(s) 140, 299
BseXI GCAGC 3 cut(s) 130, 133, 322
BshFI GGCC 2 cut(s) 90, 298
BshNI GGYRCC 1 cut(s) 172
BslI CCNNNNNNNGG 2 cut(s) 108, 182
BsnI GGCC 2 cut(s) 90, 298
BspACI CCGC 2 cut(s) 112, 308
BspANI GGCC 2 cut(s) 90, 298
BspCNI CTCAG 1 cut(s) 160
BspHI TCATGA 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 172
BsrI ACTGG 2 cut(s) 140, 299
BssECI CCNNGG 1 cut(s) 63
BssNI GRCGYC 2 cut(s) 173, 350
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 2 cut(s) 31, 260
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 2 cut(s) 173, 350
BstDEI CTNAG 1 cut(s) 168
BstENI CCTNNNNNAGG 1 cut(s) 106
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 63
BstV1I GCAGC 3 cut(s) 130, 133, 322
Bsu36I CCTNAGG 1 cut(s) 168
BsuRI GGCC 2 cut(s) 90, 298
BtsIMutI CAGTG 1 cut(s) 265
CaiI CAGNNNCTG 1 cut(s) 124
CciI TCATGA 1 cut(s) 153
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 2 cut(s) 11, 89
CviAII CATG 3 cut(s) 145, 154, 396
CviJI RGCY 9 cut(s) 5, 90, 124, 165, 192, 298, 313, 331, 345
CviKI_1 RGCY 9 cut(s) 5, 90, 124, 165, 192, 298, 313, 331, 345
DdeI CTNAG 1 cut(s) 168
DinI GGCGCC 1 cut(s) 174
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 296
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 11
Eco81I CCTNAGG 1 cut(s) 168
EcoNI CCTNNNNNAGG 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 63
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
FaeI CATG 3 cut(s) 148, 157, 399
FaiI YATR 5 cut(s) 20, 146, 155, 234, 397
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 3 cut(s) 144, 153, 395
Fnu4HI GCNGC 4 cut(s) 6, 119, 122, 311
Fsp4HI GCNGC 4 cut(s) 6, 119, 122, 311
GlaI GCGC 1 cut(s) 174
GluI GCNGC 4 cut(s) 6, 119, 122, 311
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 2 cut(s) 90, 298
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 2 cut(s) 173, 350
Hin1II CATG 3 cut(s) 148, 157, 399
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 3 cut(s) 131, 186, 379
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 256, 378
Hpy188III TCNNGA 1 cut(s) 154
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 31, 260
HpyCH4IV ACGT 2 cut(s) 302, 350
HpyCH4V TGCA 2 cut(s) 118, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 2 cut(s) 302, 350
Hsp92I GRCGYC 2 cut(s) 173, 350
Hsp92II CATG 3 cut(s) 148, 157, 399
HspAI GCGC 1 cut(s) 173
KasI GGCGCC 1 cut(s) 172
LmnI GCTCC 2 cut(s) 328, 342
LpnPI CCDG 8 cut(s) 50, 77, 105, 153, 180, 253, 280, 331
Lsp1109I GCAGC 3 cut(s) 130, 133, 322
LweI GCATC 1 cut(s) 88
MaeII ACGT 2 cut(s) 302, 350
MboII GAAGA 3 cut(s) 51, 230, 292
MlsI TGGCCA 1 cut(s) 298
MluNI TGGCCA 1 cut(s) 298
Mly113I GGCGCC 1 cut(s) 173
MlyI GAGTC 2 cut(s) 125, 373
MnlI CCTC 2 cut(s) 163, 265
Mox20I TGGCCA 1 cut(s) 298
MscI TGGCCA 1 cut(s) 298
Msp20I TGGCCA 1 cut(s) 298
MspA1I CMGCKG 1 cut(s) 124
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 118
NarI GGCGCC 1 cut(s) 173
NlaIII CATG 3 cut(s) 148, 157, 399
NlaIV GGNNCC 1 cut(s) 174
PagI TCATGA 1 cut(s) 153
PfeI GAWTC 1 cut(s) 186
PkrI GCNGC 4 cut(s) 7, 120, 123, 312
PleI GAGTC 2 cut(s) 125, 373
PluTI GGCGCC 1 cut(s) 176
PpsI GAGTC 2 cut(s) 125, 373
Psp1406I AACGTT 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 2 cut(s) 11, 89
PstNI CAGNNNCTG 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 124
SatI GCNGC 4 cut(s) 6, 119, 122, 311
Sau96I GGNCC 2 cut(s) 11, 89
SchI GAGTC 2 cut(s) 125, 373
ScrFI CCNGG 1 cut(s) 65
SetI ASST 8 cut(s) 69, 126, 163, 305, 315, 333, 347, 353
SfaNI GCATC 1 cut(s) 88
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 1 cut(s) 11
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
SsiI CCGC 2 cut(s) 112, 308
SspDI GGCGCC 1 cut(s) 172
StyD4I CCNGG 1 cut(s) 63
TaaI ACNGT 2 cut(s) 31, 260
TaiI ACGT 2 cut(s) 305, 353
TaqI TCGA 2 cut(s) 24, 243
TaqII GACCGA 1 cut(s) 28
TfiI GAWTC 1 cut(s) 186
TscAI CASTG 1 cut(s) 265
TseI GCWGC 4 cut(s) 5, 118, 121, 310
TspDTI ATGAA 2 cut(s) 142, 170
TspRI CASTG 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 11
XagI CCTNNNNNAGG 1 cut(s) 106
ZraI GACGTC 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.