Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0010016

Physical Location & Seq

Genomic Coordinates Reverse (-)

23010821 .. 23018654

Gene Structure 7834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3021 bp

ATGGCCAAGCCCGAACCTAGAGGTCGCACTGTCCTTGAACCACTGTCATTGTTCAACCTCTCTGACCACTCTCGTGCACGAGTTACATCACTCGCTATCTCCACGGTCTCTGACTCACAATGTCTAATCTACCTTGGGACCCAGTTTGGAATCCTGCTTTTGCTCTCTGTAAATCCCAATGATCAATCCCGATCTGATCCTTCCAACAACCCTTCAATCCCACAAAACATTTCGTTTTTGCGAAAAGTTTTGGTTGGCAATTCCTCAGTAGAGTCTATCCAAGTGTTTGGTGAGATTGGGAAGCTCTTGGTGCTTTTGGATGGATTTTTGTTTTTGGTTGATTCGCTTCTGTTACAGCCTGTGAAAAGATTGAGTTTCTTGAGAGGAATTTCTGTCATCACAAGGAGGCTGCGAAGCAGTGAATCAGAATGCTCGAATTTGTCAGGTCTTAGTAATTCATCAGAGTATACAAGCACAAGTCAGCGGTTTTTGCAAAAGTTCGGAAGTGGGATCAGAACAAATGGCTCGAAAACGAAAGAAACTGTGCAGCAGCGTATAGGCCATCATGTTTTCTCTATTGTGATTGGTAAAAGATTGGTATTGGTAGAGCTTGTTTTGAGTAATAGAGTAGGTAAAAGTGACCAAGACATTGATGATCGATCTTTTGTAATTCTGAAGGAAATTCAGTGTATTGATGGGGTTATGGCCATGGTATGGCTCAATGATTCGATAATTGTCAGTACGGTTAATGGATATAGTTTGTTTTCATGTGTTACCGGACAAAGTGGTGTAATATTTTCGCTACCGGATGTTTCTAGTCTGCCACGGCTTAAATTGTTGTGTAAAGAATGGAATGTGCTTTTGTTGGTGGACAATGTTGGCATCATAGCTAATGCACATGGGCAGCCGGTTGGTGGTAGCTTGGTGTTCCATAGTAACCTGGATTCTATTGGGGAGATATCTTCATATGTGGTCATTGCAAGGGATGGAAAGCTGGAGTTGTATCATAAGAAAACAGGTAGATGTATTCAGATGATCACTTTTGGTGGTGAAGGGGTAGGAGGGCCTTGCATTGTTGCAGATGAGGAAGATGGAAGTGGAAAACTTCTTGTTGTTGCAACCCCCACCAAGGTTGTTTGCTATCGGAAATTACCATCTGAAGAACAAATTAAAGATCTATTGAGAAAAAAGAACTTCAAGGAAGCCATCTCCTTGGTGGAGGATCTTGAGTGTGAGGGTGAATTATCAAAGGATATGCTCTCGTTTGTTCATGCTCAAGTGGGGTTTCTCTTGCTTTTTGACTTGCATTTTGAGGAAGCAGTGAATCACTTTTTGCAGTCTGAGACAATGCAGCCTTCTGAAGTATTTCCATTCATAATGAGAGATCCGAATCGATGGTCATTACTGGTTCCTAGAAACCGCTATTGGGGTTTGCATCCTCCCCCCGCGCCTCTGGAAGATGTTGTGGATGATGGGTTGATGGCAATTCAAAGAGCAATCTTTCTTAGGAAAGCAGGTGTAGAAACTGTGGTAGATGATGCATTTCTCCTGAATCCACCAAATAGGGATAAATTGTTGGAGTCTGCCATCAAATCAATTACAAGGTATTTGGAGGTTTCTCGTGAGAAAGAGTTGACTCCATCAGTGAAGGAGGGAGTTGACACCCTATTAATGTACCTCTACAGAGCCTTGAATAATGTTTATGACATGGAGAAGCTGGCATCATCAGAGAATTCTTGTGTTGTGGAGGAGTTGGAAACTTTGTTAGATGACTCCGGGCATTTACGTACGCTTGCCTTCCTATATGCAAGTAAAGGAATTAGCTCAAAGGCTCTTGGTATCTGGCGCATCTTGGCAAGAAATTATTCATCTGGCCTTTGGAAAGACCCCATGTTGGAGAGTGGTTCACAGGATGGTGGTACAAATATCATCTCTGGTAAGGAGACTGCTGCAGCTGAAGCATCAAAGCTTCTCGAGGAGTCATCTGATCCAAACCTTGTTCTTCAACATCTTGGATGGGTTGCAGATATCAACCAGGTTTTTGCAGTTCAGGTTTTGACATCAGAGAAAAGAGCTAATCAACTTCCACCAGATGAAGTTATTGCAGCTATTGATCCAAAGAAGGTGGAGATTTTTCAAAGGTACCTTCAGTGGTTGATTGAAGACCAAGAATACAGTGACAGTCAGTTCCACACATTATATGCTCTCTCACTTGCCAAGTCAGCAATTGAAGCATATCAAGCAGATATTGCCTCGCAAAACCTTGGTCCTGGAAGGATAGAACAGACAAACATTTCTGATGATGGAACAAGTTTGATATTTCAAAGTCCTGTTCGGGAAAGACTGCAGATGTTTTTAGAGTCTTCAGACTTGTATGACCCAGAGGAGGTTCTAGACTTAATCGAGGGATCAGAATTGTGGTCGGAGAAGGCTATTCTTTACAAGAAACTAGGGCAAGAGGCATTGGTGCTCCAAATTTTAGCCTTGAAGCTGGAAAACAGTGAAGCTGCTGAGCAATATTGTGCTGAGATTGGGAGACCTGATGTCTATATGCAGTTACTTGATATGTATTTGGATCCGCAAGATGGTAAGGAGCCTATGTTTAAAGCTGCTGTTCGCCTACTCCACAATCATGGGGAATCAATCGATCCTTTGCAAGTTTTAGAGAGATTGTCCCCAGATATGCCTCTTGAACTTGCTTCTGAGACTATACTAAGAATGCTGAGAGCTCGACTTCATCATCACCGTCAAGGAAGGATTGTGCACAATCTGTCACGTGCTTTAGATACAGATGCAAGTTTAGCAATATTGGAGGAAAAGTCAAGGCATGTGCAGATCAATGATGAAAGCCTTTGTGATTCTTGCCATGCACGCCTTGGAACTAAACTATTTGCTATGTACCCGGATGACACCATAGTCTGTTACAAGTGTTTCCGTCGTCAGGGTGAGTCTACATCAGTCACTGGTCGCAACTTTAAACAAGATGTCCTAGTGAAACCAGGTTGGCTTGTGACCCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0023052 GO:0046332 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

CNH Clathrin Vps39_1 Vps39_2

Protein Analysis

1007

Amino Acids

112.27

Weight (kDa)

5.35

Isoelectric Point (pI)

42.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps39_1	PF10366	553 - 620	7.7e-06	Vacuolar sorting protein 39 domain 1
Clathrin	PF00637	780 - 860	6.6e-08	Region in Clathrin and VPS
zf-Vps39_C	PF10367	941 - 981	1.3e-16	Vacuolar sorting protein 39 C-terminal zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22860
fragaria_vesca	FvH4_3g11790
malus_domestica	MD05G1259300.v1.1 MD10G1238900.v1.1
prunus_persica	Prupe.4G105500_v2.0.a1 Prupe.4G105500_v2.0.a1 Prupe.4G105500_v2.0.a1
pyrus_communis	pycom05g24370 pycom10g19990
rosa_chinensis	RchiOBHm_Chr5g0019211
rosa_laevigata	RLG00000032428
rosa_multiflora	Rmu_co8432961.1_g000001 Rmu_sc0002561.1_g000003
rosa_roxburghii	Rroxscaffold_1G00058780 Rroxscaffold_1G00074160
rosa_rugosa	Rorug05G0048300
rosa_samantha	Rh5AG139700 Rh5BG138700 Rh5CG150100 Rh5DG139300
rosa_wichuraiana	Rw5G012420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1505
AasI	GACNNNNNNGTC	1 cut(s)	2915
Acc36I	ACCTGC	1 cut(s)	1505
Acc65I	GGTACC	1 cut(s)	2142
AccB1I	GGYRCC	1 cut(s)	2142
AccB7I	CCANNNNNTGG	1 cut(s)	714
AccI	GTMKAC	2 cut(s)	467, 2951
AccII	CGCG	1 cut(s)	1448
AciI	CCGC	4 cut(s)	484, 1420, 1446, 2579
AcoI	YGGCCR	2 cut(s)	3, 705
AcsI	RAATTY	5 cut(s)	387, 436, 681, 1732, 2475
AcuI	CTGAAG	6 cut(s)	695, 1179, 1380, 1977, 2132, 2349
AcvI	CACGTG	1 cut(s)	2777
AfaI	GTAC	6 cut(s)	742, 1676, 1789, 1921, 2144, 2900
AfiI	CCNNNNNNNGG	8 cut(s)	714, 914, 1129, 1426, 1894, 2386, 2585, 2941
AhdI	GACNNNNNGTC	1 cut(s)	2543
AjnI	CCWGG	4 cut(s)	939, 2034, 2269, 2998
AjuI	GAANNNNNNNTTGG	2 cut(s)	1408, 1440
AleI	CACNNNNGTG	1 cut(s)	72
Alw21I	GWGCWC	4 cut(s)	79, 2472, 2731, 2766
Alw26I	GTCTC	5 cut(s)	112, 1337, 1937, 2530, 2699
Alw44I	GTGCAC	2 cut(s)	75, 2762
AlwNI	CAGNNNCTG	1 cut(s)	2963
Ama87I	CYCGRG	1 cut(s)	1973
AoxI	GGCC	5 cut(s)	3, 559, 705, 1064, 1873
ApaLI	GTGCAC	2 cut(s)	75, 2762
ApoI	RAATTY	5 cut(s)	387, 436, 681, 1732, 2475
ArsI	GACNNNNNNTTYG	6 cut(s)	1292, 1324, 1382, 1414, 2251, 2283
AseI	ATTAAT	1 cut(s)	1670
Asp700I	GAANNNNTTC	2 cut(s)	1365, 1864
Asp718I	GGTACC	1 cut(s)	2142
AspLEI	GCGC	2 cut(s)	1450, 1848
AspS9I	GGNCC	3 cut(s)	138, 1064, 2267
AsuC2I	CCSGG	3 cut(s)	1777, 2903, 3016
AsuHPI	GGTGA	5 cut(s)	302, 1061, 1250, 2735, 2957
AvaI	CYCGRG	1 cut(s)	1973
AvaII	GGWCC	2 cut(s)	138, 2267
BaeGI	GKGCMC	2 cut(s)	79, 2766
BalI	TGGCCA	2 cut(s)	5, 707
BamHI	GGATCC	1 cut(s)	2575
BanI	GGYRCC	1 cut(s)	2142
BanII	GRGCYC	1 cut(s)	2731
BauI	CACGAG	3 cut(s)	72, 78, 1620
BbrPI	CACGTG	1 cut(s)	2777
BbsI	GAAGAC	2 cut(s)	2169, 2355
Bbv12I	GWGCWC	4 cut(s)	79, 2472, 2731, 2766
BceAI	ACGGC	1 cut(s)	842
BciT130I	CCWGG	4 cut(s)	941, 2036, 2271, 3000
BclI	TGATCA	2 cut(s)	181, 1035
BcnI	CCSGG	3 cut(s)	1777, 2903, 3016
BcoDI	GTCTC	5 cut(s)	112, 1337, 1937, 2530, 2699
BfaI	CTAG	6 cut(s)	18, 816, 1413, 2393, 2450, 2990
BfmI	CTRYAG	3 cut(s)	1681, 1950, 2345
BfuAI	ACCTGC	1 cut(s)	1505
BglII	AGATCT	1 cut(s)	1174
BlpI	GCTNAGC	1 cut(s)	2511
Bme1390I	CCNGG	7 cut(s)	941, 1777, 2036, 2271, 2903, 3000, 3016
Bme18I	GGWCC	2 cut(s)	138, 2267
BmeRI	GACNNNNNGTC	1 cut(s)	2543
BmeT110I	CYCGRG	1 cut(s)	1973
BmgT120I	GGNCC	3 cut(s)	138, 1064, 2267
BmiI	GGNNCC	6 cut(s)	139, 140, 1410, 2144, 2577, 2595
BmrFI	CCNGG	7 cut(s)	941, 1777, 2036, 2271, 2903, 3000, 3016
BmrI	ACTGGG	1 cut(s)	136
BmsI	GCATC	7 cut(s)	891, 1444, 1528, 1730, 1857, 1970, 2782
BmuI	ACTGGG	1 cut(s)	136
BpiI	GAAGAC	2 cut(s)	2169, 2355
BpmI	CTGGAG	1 cut(s)	1016
Bpu1102I	GCTNAGC	1 cut(s)	2511
BpuEI	CTTGAG	3 cut(s)	400, 1247, 1260
BpuMI	CCSGG	3 cut(s)	1777, 2903, 3016
Bsa29I	ATCGAT	3 cut(s)	658, 1393, 2646
BsaAI	YACGTR	2 cut(s)	1787, 2777
BsaBI	GATNNNNATC	1 cut(s)	1389
BsaI	GGTCTC	2 cut(s)	112, 2530
BsaJI	CCNNGG	8 cut(s)	102, 133, 708, 824, 1128, 1212, 2263, 2875
BsaWI	WCCGGW	2 cut(s)	776, 805
BsaXI	ACNNNNNCTCC	4 cut(s)	2585, 2615, 2804, 2834
Bsc4I	CCNNNNNNNGG	8 cut(s)	714, 914, 1129, 1426, 1894, 2386, 2585, 2941
Bse118I	RCCGGY	1 cut(s)	907
Bse1I	ACTGG	3 cut(s)	142, 1410, 2968
Bse3DI	GCAATG	1 cut(s)	975
Bse8I	GATNNNNATC	1 cut(s)	1389
BseBI	CCWGG	4 cut(s)	941, 2036, 2271, 3000
BseCI	ATCGAT	3 cut(s)	658, 1393, 2646
BseDI	CCNNGG	8 cut(s)	102, 133, 708, 824, 1128, 1212, 2263, 2875
BseGI	GGATG	8 cut(s)	325, 814, 991, 1435, 1474, 1918, 2021, 2911
BseJI	GATNNNNATC	1 cut(s)	1389
BseLI	CCNNNNNNNGG	8 cut(s)	714, 914, 1129, 1426, 1894, 2386, 2585, 2941
BseMI	GCAATG	1 cut(s)	975
BseMII	CTCAG	6 cut(s)	279, 1332, 2502, 2517, 2694, 2714
BseNI	ACTGG	3 cut(s)	142, 1410, 2968
BseRI	GAGGAG	3 cut(s)	1763, 1991, 2399
BseSI	GKGCMC	2 cut(s)	79, 2766
BsgI	GTGCAG	2 cut(s)	566, 2852
Bsh1236I	CGCG	1 cut(s)	1448
BshFI	GGCC	5 cut(s)	5, 561, 707, 1066, 1875
BshNI	GGYRCC	1 cut(s)	2142
BshVI	ATCGAT	3 cut(s)	658, 1393, 2646
BsiHKAI	GWGCWC	4 cut(s)	79, 2472, 2731, 2766
BsiHKCI	CYCGRG	1 cut(s)	1973
BsiSI	CCGG	6 cut(s)	777, 806, 908, 1776, 2903, 3016
BsiWI	CGTACG	1 cut(s)	1787
BslFI	GGGAC	2 cut(s)	151, 2659
BslI	CCNNNNNNNGG	8 cut(s)	714, 914, 1129, 1426, 1894, 2386, 2585, 2941
BsmAI	GTCTC	5 cut(s)	112, 1337, 1937, 2530, 2699
BsmFI	GGGAC	2 cut(s)	151, 2659
BsmI	GAATGC	2 cut(s)	434, 2724
BsnI	GGCC	5 cut(s)	5, 561, 707, 1066, 1875
Bso31I	GGTCTC	2 cut(s)	112, 2530
BsoBI	CYCGRG	1 cut(s)	1973
Bsp1286I	GDGCHC	4 cut(s)	79, 2472, 2731, 2766
Bsp1720I	GCTNAGC	1 cut(s)	2511
Bsp19I	CCATGG	1 cut(s)	708
BspACI	CCGC	4 cut(s)	484, 1420, 1446, 2579
BspANI	GGCC	5 cut(s)	5, 561, 707, 1066, 1875
BspCNI	CTCAG	6 cut(s)	278, 1333, 2503, 2518, 2695, 2715
BspDI	ATCGAT	3 cut(s)	658, 1393, 2646
BspFNI	CGCG	1 cut(s)	1448
BspLI	GGNNCC	6 cut(s)	139, 140, 1410, 2144, 2577, 2595
BspMAI	CTGCAG	2 cut(s)	1954, 2349
BspMI	ACCTGC	1 cut(s)	1505
BspT107I	GGYRCC	1 cut(s)	2142
BspTNI	GGTCTC	2 cut(s)	112, 2530
BsrDI	GCAATG	1 cut(s)	975
BsrFI	RCCGGY	1 cut(s)	907
BsrI	ACTGG	3 cut(s)	142, 1410, 2968
BssAI	RCCGGY	1 cut(s)	907
BssECI	CCNNGG	8 cut(s)	102, 133, 708, 824, 1128, 1212, 2263, 2875
BssNAI	GTATAC	1 cut(s)	468
BssSI	CACGAG	3 cut(s)	72, 78, 1620
BssT1I	CCWWGG	6 cut(s)	133, 708, 1128, 1212, 2263, 2875
Bst1107I	GTATAC	1 cut(s)	468
Bst2BI	CACGAG	3 cut(s)	72, 78, 1620
Bst2UI	CCWGG	4 cut(s)	941, 2036, 2271, 3000
BstAPI	GCANNNNNTGC	1 cut(s)	2249
BstBAI	YACGTR	2 cut(s)	1787, 2777
BstC8I	GCNNGC	3 cut(s)	1719, 1794, 2872
BstDEI	CTNAG	9 cut(s)	265, 449, 1341, 1505, 2511, 2526, 2703, 2714, 2723
BstDSI	CCRYGG	3 cut(s)	102, 708, 824
BstF5I	GGATG	8 cut(s)	325, 814, 991, 1435, 1474, 1918, 2021, 2911
BstFNI	CGCG	1 cut(s)	1448
BstHHI	GCGC	2 cut(s)	1450, 1848
BstMAI	GTCTC	5 cut(s)	112, 1337, 1937, 2530, 2699
BstMWI	GCNNNNNNNGC	9 cut(s)	310, 490, 1958, 2222, 2231, 2240, 2249, 2801, 2871
BstNI	CCWGG	4 cut(s)	941, 2036, 2271, 3000
BstNSI	RCATGY	1 cut(s)	2831
BstSCI	CCNGG	7 cut(s)	939, 1775, 2034, 2269, 2901, 2998, 3014
BstSFI	CTRYAG	3 cut(s)	1681, 1950, 2345
BstSLI	GKGCMC	2 cut(s)	79, 2766
BstSNI	TACGTA	1 cut(s)	1787
BstUI	CGCG	1 cut(s)	1448
BstV2I	GAAGAC	2 cut(s)	2169, 2355
BstX2I	RGATCY	4 cut(s)	1174, 1222, 1384, 2575
BstXI	CCANNNNNNTGG	3 cut(s)	287, 1213, 2633
BstYI	RGATCY	4 cut(s)	1174, 1222, 1384, 2575
BstZ17I	GTATAC	1 cut(s)	468
Bsu15I	ATCGAT	3 cut(s)	658, 1393, 2646
BsuRI	GGCC	5 cut(s)	5, 561, 707, 1066, 1875
BsuTUI	ATCGAT	3 cut(s)	658, 1393, 2646
BtgI	CCRYGG	3 cut(s)	102, 708, 824
BtsCI	GGATG	8 cut(s)	325, 814, 991, 1435, 1474, 1918, 2021, 2911
BtsI	GCAGTG	2 cut(s)	424, 1326
BveI	ACCTGC	1 cut(s)	1505
Cac8I	GCNNGC	3 cut(s)	1719, 1794, 2872
CaiI	CAGNNNCTG	1 cut(s)	2963
CfoI	GCGC	2 cut(s)	1450, 1848
Cfr10I	RCCGGY	1 cut(s)	907
Cfr13I	GGNCC	3 cut(s)	138, 1064, 2267
ClaI	ATCGAT	3 cut(s)	658, 1393, 2646
CsiI	ACCWGGT	2 cut(s)	2034, 2998
Csp6I	GTAC	6 cut(s)	741, 1675, 1788, 1920, 2143, 2899
CspCI	CAANNNNNGTGG	6 cut(s)	30, 65, 1114, 1149, 2106, 2141
CviQI	GTAC	6 cut(s)	741, 1675, 1788, 1920, 2143, 2899
DdeI	CTNAG	9 cut(s)	265, 449, 1341, 1505, 2511, 2526, 2703, 2714, 2723
DraI	TTTAAA	2 cut(s)	2605, 2977
DrdI	GACNNNNNNGTC	1 cut(s)	2915
DriI	GACNNNNNGTC	1 cut(s)	2543
DseDI	GACNNNNNNGTC	1 cut(s)	2915
EaeI	YGGCCR	2 cut(s)	3, 705
Eam1105I	GACNNNNNGTC	1 cut(s)	2543
Ecl136II	GAGCTC	1 cut(s)	2729
Eco105I	TACGTA	1 cut(s)	1787
Eco130I	CCWWGG	6 cut(s)	133, 708, 1128, 1212, 2263, 2875
Eco24I	GRGCYC	1 cut(s)	2731
Eco31I	GGTCTC	2 cut(s)	112, 2530
Eco32I	GATATC	2 cut(s)	960, 2029
Eco47I	GGWCC	2 cut(s)	138, 2267
Eco53kI	GAGCTC	1 cut(s)	2729
Eco57I	CTGAAG	6 cut(s)	695, 1179, 1380, 1977, 2132, 2349
Eco72I	CACGTG	1 cut(s)	2777
Eco88I	CYCGRG	1 cut(s)	1973
EcoICRI	GAGCTC	1 cut(s)	2729
EcoO109I	RGGNCCY	2 cut(s)	138, 1064
EcoRI	GAATTC	1 cut(s)	1732
EcoRII	CCWGG	4 cut(s)	939, 2034, 2269, 2998
EcoRV	GATATC	2 cut(s)	960, 2029
EcoT14I	CCWWGG	6 cut(s)	133, 708, 1128, 1212, 2263, 2875
EcoT22I	ATGCAT	1 cut(s)	1543
EcoT38I	GRGCYC	1 cut(s)	2731
ErhI	CCWWGG	6 cut(s)	133, 708, 1128, 1212, 2263, 2875
FaqI	GGGAC	2 cut(s)	151, 2659
FauI	CCCGC	1 cut(s)	1453
FauNDI	CATATG	1 cut(s)	967
FbaI	TGATCA	2 cut(s)	181, 1035
FblI	GTMKAC	2 cut(s)	467, 2951
FokI	GGATG	8 cut(s)	332, 821, 998, 1422, 1481, 1925, 2028, 2918
FriOI	GRGCYC	1 cut(s)	2731
FspBI	CTAG	6 cut(s)	18, 816, 1413, 2393, 2450, 2990
GlaI	GCGC	2 cut(s)	1449, 1847
GsuI	CTGGAG	1 cut(s)	1016
HaeIII	GGCC	5 cut(s)	5, 561, 707, 1066, 1875
HapII	CCGG	6 cut(s)	777, 806, 908, 1776, 2903, 3016
HhaI	GCGC	2 cut(s)	1450, 1848
Hin6I	GCGC	2 cut(s)	1448, 1846
HinP1I	GCGC	2 cut(s)	1448, 1846
HincII	GTYRAC	2 cut(s)	1635, 1660
HindII	GTYRAC	2 cut(s)	1635, 1660
HindIII	AAGCTT	1 cut(s)	1967
HpaII	CCGG	6 cut(s)	777, 806, 908, 1776, 2903, 3016
HphI	GGTGA	5 cut(s)	302, 1061, 1250, 2735, 2957
Hpy166II	GTNNAC	8 cut(s)	77, 468, 871, 1635, 1660, 1907, 2764, 2952
Hpy8I	GTNNAC	8 cut(s)	77, 468, 871, 1635, 1660, 1907, 2764, 2952
Hpy99I	CGWCG	1 cut(s)	2940
HpyCH4IV	ACGT	2 cut(s)	1786, 2776
HpyF10VI	GCNNNNNNNGC	9 cut(s)	310, 490, 1958, 2222, 2231, 2240, 2249, 2801, 2871
HpyF3I	CTNAG	9 cut(s)	265, 449, 1341, 1505, 2511, 2526, 2703, 2714, 2723
HpySE526I	ACGT	2 cut(s)	1786, 2776
HspAI	GCGC	2 cut(s)	1448, 1846
KflI	GGGWCCC	1 cut(s)	138
KpnI	GGTACC	1 cut(s)	2146
Ksp22I	TGATCA	2 cut(s)	181, 1035
LmnI	GCTCC	2 cut(s)	2475, 2593
LweI	GCATC	7 cut(s)	891, 1444, 1528, 1730, 1857, 1970, 2782
MabI	ACCWGGT	2 cut(s)	2034, 2998
MaeI	CTAG	6 cut(s)	18, 816, 1413, 2393, 2450, 2990
MaeII	ACGT	2 cut(s)	1786, 2776
MboII	GAAGA	7 cut(s)	954, 1100, 1172, 1469, 1994, 2174, 2355
MfeI	CAATTG	1 cut(s)	2226
MflI	RGATCY	4 cut(s)	1174, 1222, 1384, 2575
MhlI	GDGCHC	4 cut(s)	79, 2472, 2731, 2766
MlsI	TGGCCA	2 cut(s)	5, 707
MluNI	TGGCCA	2 cut(s)	5, 707
MlyI	GAGTC	8 cut(s)	107, 281, 1589, 1630, 1766, 1988, 2369, 2957
MmeI	TCCRAC	5 cut(s)	228, 1557, 1734, 1875, 2403
Mox20I	TGGCCA	2 cut(s)	5, 707
Mph1103I	ATGCAT	1 cut(s)	1543
MroXI	GAANNNNTTC	2 cut(s)	1365, 1864
MscI	TGGCCA	2 cut(s)	5, 707
MseI	TTAA	7 cut(s)	747, 831, 1170, 1670, 2399, 2604, 2976
MslI	CAYNNNNRTG	2 cut(s)	72, 2631
Msp20I	TGGCCA	2 cut(s)	5, 707
MspA1I	CMGCKG	2 cut(s)	484, 1955
MspI	CCGG	6 cut(s)	777, 806, 908, 1776, 2903, 3016
MspR9I	CCNGG	7 cut(s)	941, 1777, 2036, 2271, 2903, 3000, 3016
MunI	CAATTG	1 cut(s)	2226
Mva1269I	GAATGC	2 cut(s)	434, 2724
MvaI	CCWGG	4 cut(s)	941, 2036, 2271, 3000
MvnI	CGCG	1 cut(s)	1448
MwoI	GCNNNNNNNGC	9 cut(s)	310, 490, 1958, 2222, 2231, 2240, 2249, 2801, 2871
NciI	CCSGG	3 cut(s)	1777, 2903, 3016
NcoI	CCATGG	1 cut(s)	708
NdeI	CATATG	1 cut(s)	967
NlaIV	GGNNCC	6 cut(s)	139, 140, 1410, 2144, 2577, 2595
NmuCI	GTSAC	5 cut(s)	638, 2177, 2772, 2959, 3010
NsiI	ATGCAT	1 cut(s)	1543
NspI	RCATGY	1 cut(s)	2831
OliI	CACNNNNGTG	1 cut(s)	72
PaeR7I	CTCGAG	1 cut(s)	1973
PaqCI	CACCTGC	1 cut(s)	1505
PctI	GAATGC	2 cut(s)	434, 2724
PdmI	GAANNNNTTC	2 cut(s)	1365, 1864
Pfl23II	CGTACG	1 cut(s)	1787
PflMI	CCANNNNNTGG	1 cut(s)	714
PfoI	TCCNGGA	1 cut(s)	2269
PleI	GAGTC	8 cut(s)	107, 280, 1588, 1630, 1766, 1987, 2368, 2956
PmaCI	CACGTG	1 cut(s)	2777
PmlI	CACGTG	1 cut(s)	2777
PpsI	GAGTC	8 cut(s)	107, 280, 1588, 1630, 1766, 1987, 2368, 2956
Ppu21I	YACGTR	2 cut(s)	1787, 2777
PpuMI	RGGWCCY	1 cut(s)	138
PshBI	ATTAAT	1 cut(s)	1670
Psp124BI	GAGCTC	1 cut(s)	2731
Psp5II	RGGWCCY	1 cut(s)	138
Psp6I	CCWGG	4 cut(s)	939, 2034, 2269, 2998
PspCI	CACGTG	1 cut(s)	2777
PspGI	CCWGG	4 cut(s)	939, 2034, 2269, 2998
PspLI	CGTACG	1 cut(s)	1787
PspN4I	GGNNCC	6 cut(s)	139, 140, 1410, 2144, 2577, 2595
PspPI	GGNCC	3 cut(s)	138, 1064, 2267
PspPPI	RGGWCCY	1 cut(s)	138
PstI	CTGCAG	2 cut(s)	1954, 2349
PstNI	CAGNNNCTG	1 cut(s)	2963
PsuI	RGATCY	4 cut(s)	1174, 1222, 1384, 2575
PvuII	CAGCTG	1 cut(s)	1955
RsaI	GTAC	6 cut(s)	742, 1676, 1789, 1921, 2144, 2900
RsaNI	GTAC	6 cut(s)	741, 1675, 1788, 1920, 2143, 2899
RseI	CAYNNNNRTG	2 cut(s)	72, 2631
SacI	GAGCTC	1 cut(s)	2731
SaqAI	TTAA	7 cut(s)	747, 831, 1170, 1670, 2399, 2604, 2976
Sau96I	GGNCC	3 cut(s)	138, 1064, 2267
SchI	GAGTC	8 cut(s)	107, 281, 1589, 1630, 1766, 1988, 2369, 2957
ScrFI	CCNGG	7 cut(s)	941, 1777, 2036, 2271, 2903, 3000, 3016
SduI	GDGCHC	4 cut(s)	79, 2472, 2731, 2766
SexAI	ACCWGGT	2 cut(s)	2034, 2998
SfaNI	GCATC	7 cut(s)	891, 1444, 1528, 1730, 1857, 1970, 2782
SfcI	CTRYAG	3 cut(s)	1681, 1950, 2345
Sfr274I	CTCGAG	1 cut(s)	1973
SinI	GGWCC	2 cut(s)	138, 2267
SlaI	CTCGAG	1 cut(s)	1973
SmiMI	CAYNNNNRTG	2 cut(s)	72, 2631
SmlI	CTYRAG	4 cut(s)	379, 1226, 1275, 1973
SmoI	CTYRAG	4 cut(s)	379, 1226, 1275, 1973
SnaBI	TACGTA	1 cut(s)	1787
SsiI	CCGC	4 cut(s)	484, 1420, 1446, 2579
SspI	AATATT	3 cut(s)	795, 2519, 2808
SspMI	CTAG	6 cut(s)	18, 816, 1413, 2393, 2450, 2990
SstI	GAGCTC	1 cut(s)	2731
StyD4I	CCNGG	7 cut(s)	939, 1775, 2034, 2269, 2901, 2998, 3014
StyI	CCWWGG	6 cut(s)	133, 708, 1128, 1212, 2263, 2875
TaiI	ACGT	2 cut(s)	1789, 2779
TaqI	TCGA	9 cut(s)	434, 527, 658, 728, 1393, 1974, 2403, 2646, 2731
Tru1I	TTAA	7 cut(s)	747, 831, 1170, 1670, 2399, 2604, 2976
Tru9I	TTAA	7 cut(s)	747, 831, 1170, 1670, 2399, 2604, 2976
TseFI	GTSAC	5 cut(s)	638, 2177, 2772, 2959, 3010
Tsp45I	GTSAC	5 cut(s)	638, 2177, 2772, 2959, 3010
TspDTI	ATGAA	9 cut(s)	447, 756, 954, 1259, 1363, 1857, 2109, 2726, 2859
TspGWI	ACGGA	1 cut(s)	2924
Van91I	CCANNNNNTGG	1 cut(s)	714
VneI	GTGCAC	2 cut(s)	75, 2762
VpaK11BI	GGWCC	2 cut(s)	138, 2267
VspI	ATTAAT	1 cut(s)	1670
XapI	RAATTY	5 cut(s)	387, 436, 681, 1732, 2475
XbaI	TCTAGA	1 cut(s)	2392
XceI	RCATGY	1 cut(s)	2831
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1213, 2873
XhoI	CTCGAG	1 cut(s)	1973
XmiI	GTMKAC	2 cut(s)	467, 2951
XmnI	GAANNNNTTC	2 cut(s)	1365, 1864
XspI	CTAG	6 cut(s)	18, 816, 1413, 2393, 2450, 2990
Zsp2I	ATGCAT	1 cut(s)	1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.