RLG00000032428

Transforming growth factor-beta receptor-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010016
Physical Location & Seq
Forward (+)
15073753 .. 15085867
12115 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032428

Sequence Viewer

Length: 5313 bp
ATGTTTTCGTCTATGGCTACTGCATCTGTTCAAGGTTCTGTTGCTGGTGGGCTTGGGGATCCTGATCGAATTTTTTTTGGTGGATTCCCTTATTACTTTAAAGAAGAACAGATCAGGGAGTTGGTAGAGTCCTTCGGACCCCTTCGCAGTTTTGAACTAGTGACAGATGGAGGCAGAGGAAACAACTTAGTTGCTGCCTATTGCGTTTTCCAGGATGTTTCAGTTACAGACATTGCTTGTTGTGCCCTGAATGGGATTCGAATTGGTGCCCAGACACTGATTGCTAGGCGTGCTAATCATGGTGCCAACCAGCAGCCGGAACCATATTCTTCTTCTCAAGAAAATTGCAGTATTTGCTGGTGTAGCCTGACAACTGGCCAAGGCCAAGCGATCTTTACTGCTGAGTGCTCCCACCGTTTCCACTACCCTTGTATTGCCAACAATGTCCAGCATGGAAATCTTTACTGCCCTGTCTGCAGAGTAAAATGGGACAAGAATAATGTACCTTTCCAAGTTCCTCCTCCTCAACAGACCAGGATCCGTTTCAGGCAGTTCCCAATGCCGCAGCAACCCCATTGGAATTATCCTCACCTCCCATATCAGCAGCCGCATTGGAATCATCCTCAGTACCCATTTGAGCAGCAACCCCAGGACCCTCCTGAGCCACTTACCTTCTCTGATGATGAACCTCTCCAGTCATTGACCTCACCTGTTCAATCCTCCTCCCACCAGAATGTGACAGTTAATACTTATGCAGAGTGCTCTGCTATCTCTGCTGCGGAGTCTAGCTCAAAGTTTCCTGTCCTTGTCAGCATCCGTGCACCACCCCTTAAGGATTCTGAGGGCCATGGTCGTTCTCCCATTGACCTTGTAACAGTTCTGGATGTAAGTGGCAGCATGACTGGCTCAAAGCTTAACCTTGTCAAGCATGCTGTCAAATTTGTCATAGAAAACTTGGGGTCTTCAGACAGACTTTCTATTGTCTCATTCTCATCAAATTCTAAGAGAGTTCTTCCTCTCCAAAGGATGACTGCTGATGGCCGTGAAAGTGCCATACTAGCAATCGAGTCTCTTAGAGCAGGTGGTGGAACTAACATTGCCGAAGCACTGAAGAAAGGAATTAAGGTCCTTGAAGATCGAAGGGAAAGGAACCCAGTTGCTAGCATCATCCTCTTATCCGATGGCCAAGATAACTACTGTCGAAGTGGAAGCCAAATTTTGAACCAATTGCCTGCTTCCATTCGTTCTAGTGACATGCAACAGGAAACCCCAGTCCATGCATTTGGGTTTGGCCAAGACCATGATGCAAGCATTATGCATGCTATTTCTGATGCATCCGGTGGTACCTTTTCATTCATTGAATCTGTTGGCATGATACAAGATGGTTTTGCTTTGTGCATTGGTGGTCTTCTCAGTGTAGTGGCTCAGGAGCTTCGCCTCACAGTAAGGTCAGCATCAGCTGGGGTGAAGATTGTTTCAATACCATCAGGGGGACATTTGAGCCGGATTTCTGATGAAGGCCAGCGAGGTGTAGTTGATATTGGAAATATGTATGCAGAAGAGGGGAAACAATTTCTGGTCTACCTGTTAGTTCCACCCTCAGCTTCACATGTTAAGATGCCATTGTTAGATGTAACATGTGTGTACAAAGATCTTGCTTCAAATGAGTTGATGCAGGTGCAAGGTGGGAGAGTAGAGATACTGAGACCTGAGGTTTGTTCCCCTGCAGAGAAGGCAGTGTCTTTGGAGGTTGATCGACAGCGGAACAGAATTGTGGTGGCTGAAACTATTGCAGAGGCACAAAGGTTGGCTGAGTTGGGAAACCTGGAAGTTGCACGGGCACTTTTGGCTGAACAAAGAGCAACTGTCTTGGCATCAGCAGCAGCTCAAGCTGGGGATGGTCTCAGTAATTTGTTTGAAACTGAGCTTAGAGAAGTCATGGAGAGAATGGCATCTATGGACTTGTACGCTAACTCAGGGCGTGCTTACGCTCTCGCAGGGATGAGTTCTCATTCTCGACAGAGGGCTTCAGTTAGGGGTGACACAATAACCTCATGTCTCTCTGGTGCCTCCAACCATGTGAATTTCTCCGGCGGTGGCAATGCCTTAATGCAGCAGTCAGGTGCCTCTGCTTGTTTTGGCGCCCCTGCAGGTTTCGGTGGTGCTAGTTTTTCATCTGGTGCCGCTCCAGGTGGAAGTCAATGGTCTGCTGCAGTTGAGACGCGAGAGAGAGAGTCCAGAACTCACAAACACTCCGGCATCTTCCCACCGGTGAAGCTCCGTCGTCGCTCTGTTGACTTCCGGCGATCACCCACAGCTTTCCAAGTTGTGTGGGGGTATTTTATGGTGTCTCGTGCACCAGTTAGATCCCTCGCTATCTCCAATGTGTCTGATTCCCAGTGTCTAATATACCTTGGGACTCAGTTTGGGGGGCTATTTTTGTTCTCTGTAGACAATAAGGATCTCAATGCTGCGTCTGCGTCCGATCCTTCCAATAGCCCTTCGGTCCCACAGAGCATTAAGTTTGTGCAAAATATTTTGGTTGGAAATTCCTCGGTGGACTATATACATGTGTTTGGTGAGATTGGAAAGCTGTTAGTGCTCTTGGATGGCTTTCTGTTTTTGGTTGATTCACTTCTGCTGCAGCCAGTGAAGAAGTTGAGTTTCTTGAGAGGAATTAATGTCATTACTAGGAGGTTGCGAAGTAATGAATCAGAGTGCTCAAATTTGTCAGAAGGTGCTGCCAGTTCATCAGAGTATACGAGCACAAGTCAGCGGTTTTTGAAAAAATTGGGAGGTGGGATAAGAGCAAATGGCTTGAAAATGAAAGAATCAACACAGCACCGGGTGGGCAATCATGTCTTTTCTGTCGTGATTGGTAAAAGAATGATATTGACAGAGTTTGTTTTGAGTAATAGAGTAGGTAAAAGTGACCAAGACATCGATGATGGGTCTTTTGTTATTCTGAAAGAAATTCCATGTATCGATGGGGTTATGGCCATGGTATGGCTCAATGATTCGATAATTGTGAGCACACTTAATGGGTATACTTTGTTTTCATGTGTTACGGGACAAAGTGGTGTCATATTTTCGTTACCAGATGTTTCTAGTCCACCTCGGCTTAAACTGTTGTGTAAAGAATGGAATGTTCTCTTGTTGGTGGACAACGTAGGCATCATAGCAAATGCCCACGGGCAGCCGGTTGGTGGGAGCCTCGTGTTCCATCGTGACCCAGATTCTATTGGAGAGATATCTTCATATGTGGTTGTTGCAAGGGATGGAAAGATGGAACTGTATCATAAGAAAACAGGTAGGTGTGTTCAGATGGTCACTTTTGGTGGTGAAGGGGTTGGAGGGCCTTGCATTGTTGCAGATGAGGAAGATGGAAGTGGAAAACTTATTGTCGTTGCAACACCTACCAAGGTTATTTGCTATCGGAAGTTACCTTCTGAGGAACAAATTAAAGATCTGTTGAGAAAAAAGAACTTCAAGGAAGCCATCTCCTTAGTGGAGGAGCTTGAATGTGAAGGCGAATTATCAAAAGATATGCTGTCATTTGTTCATGCTCAAGTGGGTTTTCTCTTACTTTTTGACTTGCATTTTGAGGAAGCAGTGAATCACTTTTTGCAGTCTGAGACAATGCAGCCTTCTGAAGTATTTCCATTTATAATGAGGGATCCAAATCGATGGTCGCTACTGGTTCCTAGAAACCGTTATTGGGGTTTGCATCCTCCCCCTGCACCTCTGGAAGATGTTGTGGATGACGGATTAATGGCGATCCAAAGAGCAATCTTTCTGAGGAAAGCAGGTGTAGAGACTGTGGTAGATGATGCATTTCTCCTTAATCTGCCAAGCCGAGATGATCTGTTGGAGTCAGCCATCAAGTCAATTACTAGGTATTTGGAGGTTTCCCGTGATAAAGAGTTGACTCCATCAGTGAAGGAGGGAGTTGACACACTATTAATGTATTTGTACAGAGCTTTAAATAATGTCAATGAAATGGAGAAGCTGGTATCTTCTGCAAATTCTTGTGTTGTGGAGGAGTTGGAAAGTCTATTAGATGACTCTGGGCATTTGCGAACGCTTGCCTTTCTATATTCAAGTAAAGGAATGAGTTCAAAGGCTCTTGCTATCTGGCGCATTTTGGCAAGAAATTATTCATCTGGCCTTTGGAAAGATCCTTCTTCAGAGAGTAGCTCAGATTGTGGTGGTCCAAATATTATCTCTGGTAAAGAGACTGCTGCAGCTGAGGCATCAAAGATTCTTGAGGAGTCATCAGATTCACAGCTCGTTCTACAACATCTTGGATGGGTTGCAGATATCAACCAGGTCTTTGCTGTTCAGATTTTAACATCAGAGAAAAGAGACAATCAGCTCCCACCAGAGGAAGTAATTGCAGCTATTGATCCAAAGAAAGTTGAAATACTTCAAAGGTACCTTCAGTGGTTGATTGAAGACCAAGATTCTAACGACAGTCAGTTCCACACAATATATGCTCTCTCACTTGCTAAGTCAGCCCTTGAATCATTCGAAGCAGAAGTTGCCTCCAAAATCCTCGATCATGTAAGGAGAGAGGAAACAAGCATTTCTGAATATAGAACCAGTGCAATATTTCAAAGTCCTGTGCGGGAAAGACTCCAGATTTTTTTACTGTCTTCAGACTTGTATGACCCAGAGGAAGTTCTTGACTTGATTGAAGGATCAGAATTATGGTCAGAGAAGGCTATACTATACAAAAAACTAGGGCAAGAGGCACTGGTACTCCAAATTTTGGCCTTGAAGCTAGAAGACAGTGAAGCTGCTGAGCAATATTGTGCTGAGATTGGCAGACCAGATGTCTATATGCAGTTACTTGATATGTATTTGGATCCACAAGATGGTAAGGAGCCTATGTTTAAAGCTGCTGTTCGCCTACTCCACAATCATGGAGAATCGTTGGATCCTTTGCAAGTATTAGAGAGATTGTCCCCAGATATGCCCCTTCAACTTGCTTCAGAGACTATATTAAGAATGTTGAGAGCTAGACTTCATCATCATCGTCAAGGACGTATTGTGCATAATCTGGCCCGTGCCTTAGATACTGATGCAAGTTTAGCAATATTAGAGGAAAGATCAAGGCATGTGCAGATCAATGATGAAAGCCTTTGCGATTCTTGTCATGCACGCCTGGGAACTAAACTTTTTGCAATGTACCCTGATGACACCATAGTTTGTTATAAGTGTTTTCGTCGCCAGGGTGAGTCTACTTCAGTCACTGGTCGCAACTTTAAACAAGATGTCCTAGTAAAACCTGGTTGGCTCGTGACCCCGTAA

Protein Analysis

1771

Amino Acids

194.65

Weight (kDa)

5.67

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 24 - 92 6.4e-07 RNA recognition motif
zf-RING_11 PF17123 115 - 145 6.4e-09 RING-like zinc finger
zf-RING_2 PF13639 115 - 160 2e-06 Ring finger domain
VWA_CoxE PF05762 285 - 402 2.4e-07 VWA domain containing CoxE-like protein
VWA PF00092 289 - 453 3.2e-23 von Willebrand factor type A domain
VWA_3 PF13768 289 - 452 4.7e-19 von Willebrand factor type A domain
VWA_2 PF13519 290 - 396 7.7e-19 von Willebrand factor type A domain
Vwaint PF14624 640 - 681 1.3e-09 VWA / Hh protein intein-like
Vps39_1 PF10366 1317 - 1385 3e-07 Vacuolar sorting protein 39 domain 1
Clathrin PF00637 1473 - 1624 1e-06 Region in Clathrin and VPS
zf-Vps39_C PF10367 1705 - 1745 2.5e-16 Vacuolar sorting protein 39 C-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3668, 5217
AarI CACCTGC 3 cut(s) 1070, 1666, 3797
Acc36I ACCTGC 4 cut(s) 1070, 1666, 2141, 3797
Acc65I GGTACC 2 cut(s) 1343, 4434
AccB1I GGYRCC 8 cut(s) 266, 302, 1343, 2066, 2123, 2141, 2180, 4434
AccB7I CCANNNNNTGG 3 cut(s) 3006, 4771, 4877
AccBSI CCGCTC 1 cut(s) 2186
AccI GTMKAC 5 cut(s) 1581, 2451, 2759, 3047, 5243
AccII CGCG 1 cut(s) 2224
AciI CCGC 8 cut(s) 563, 608, 779, 1762, 2094, 2184, 2776, 4627
AcoI YGGCCR 5 cut(s) 376, 1039, 1183, 1291, 2997
AcuI CTGAAG 9 cut(s) 948, 1130, 2013, 3672, 4170, 4424, 4641, 4977, 5232
AcyI GRCGYC 1 cut(s) 2142
AdeI CACNNNGTG 1 cut(s) 2848
AfaI GTAC 9 cut(s) 504, 629, 1345, 1646, 1967, 3974, 4436, 4761, 5192
AfiI CCNNNNNNNGG 9 cut(s) 252, 316, 1490, 3006, 3206, 3718, 4384, 4771, 4877
AflII CTTAAG 1 cut(s) 830
AflIII ACRYGT 3 cut(s) 1609, 1637, 2569
AgeI ACCGGT 1 cut(s) 2269
AhdI GACNNNNNGTC 1 cut(s) 4835
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 9 cut(s) 210, 533, 648, 1824, 2188, 4326, 5166, 5232, 5290
AjuI GAANNNNNNNTTGG 2 cut(s) 3700, 3732
Alw21I GWGCWC 8 cut(s) 410, 764, 823, 2359, 2604, 2723, 2768, 3035
Alw44I GTGCAC 2 cut(s) 819, 2355
AlwNI CAGNNNCTG 2 cut(s) 277, 5255
ApaLI GTGCAC 2 cut(s) 819, 2355
ArsI GACNNNNNNTTYG 6 cut(s) 1698, 1730, 3584, 3616, 3674, 3706
AseI ATTAAT 3 cut(s) 2679, 3770, 3962
AsiGI ACCGGT 1 cut(s) 2269
Asp700I GAANNNNTTC 4 cut(s) 3657, 4114, 4156, 4425
Asp718I GGTACC 2 cut(s) 1343, 4434
AspLEI GCGC 2 cut(s) 2144, 4140
AspS9I GGNCC 8 cut(s) 137, 652, 844, 1126, 2506, 3356, 4211, 5065
AsuC2I CCSGG 1 cut(s) 2846
AsuHPI GGTGA 9 cut(s) 581, 699, 1477, 2051, 2284, 2301, 2591, 3353, 5249
AsuII TTCGAA 2 cut(s) 259, 4530
AsuNHI GCTAGC 1 cut(s) 1160
AvaII GGWCC 5 cut(s) 137, 652, 1126, 2506, 4211
AxyI CCTNAGG 1 cut(s) 1710
BaeGI GKGCMC 5 cut(s) 247, 271, 823, 1843, 2359
BalI TGGCCA 4 cut(s) 378, 1185, 1293, 2999
BamHI GGATCC 5 cut(s) 58, 537, 3676, 4867, 4939
BanI GGYRCC 8 cut(s) 266, 302, 1343, 2066, 2123, 2141, 2180, 4434
BauI CACGAG 3 cut(s) 2352, 3215, 5300
BbsI GAAGAC 5 cut(s) 954, 1400, 4461, 4647, 4794
Bbv12I GWGCWC 8 cut(s) 410, 764, 823, 2359, 2604, 2723, 2768, 3035
BbvCI CCTCAGC 2 cut(s) 1600, 4248
BceAI ACGGC 1 cut(s) 1026
BciT130I CCWGG 9 cut(s) 212, 535, 650, 1826, 2190, 4328, 5168, 5234, 5292
BcnI CCSGG 1 cut(s) 2846
BcuI ACTAGT 1 cut(s) 157
BfmI CTRYAG 7 cut(s) 475, 1725, 2148, 2211, 2448, 2642, 4242
BfoI RGCGCY 1 cut(s) 2145
BfrI CTTAAG 1 cut(s) 830
BfuAI ACCTGC 4 cut(s) 1070, 1666, 2141, 3797
BglII AGATCT 2 cut(s) 1651, 3466
BlpI GCTNAGC 1 cut(s) 4803
Bme18I GGWCC 5 cut(s) 137, 652, 1126, 2506, 4211
BmeRI GACNNNNNGTC 1 cut(s) 4835
BmgT120I GGNCC 8 cut(s) 137, 652, 844, 1126, 2506, 3356, 4211, 5065
BmrI ACTGGG 3 cut(s) 1148, 1265, 2392
BmtI GCTAGC 1 cut(s) 1164
BmuI ACTGGG 3 cut(s) 1148, 1265, 2392
BpiI GAAGAC 5 cut(s) 954, 1400, 4461, 4647, 4794
BplI GAGNNNNNCTC 4 cut(s) 773, 805, 4181, 4213
BpmI CTGGAG 3 cut(s) 677, 2172, 4622
Bpu10I CCTNAGC 4 cut(s) 660, 1425, 1600, 4248
Bpu1102I GCTNAGC 1 cut(s) 4803
Bpu14I TTCGAA 2 cut(s) 259, 4530
BpuEI CTTGAG 5 cut(s) 321, 1872, 2689, 3552, 4286
BpuMI CCSGG 1 cut(s) 2846
Bsa29I ATCGAT 3 cut(s) 2943, 2985, 3685
BsaBI GATNNNNATC 2 cut(s) 63, 3681
BsaHI GRCGYC 1 cut(s) 2142
BsaI GGTCTC 2 cut(s) 1699, 1907
BsaWI WCCGGW 2 cut(s) 1337, 2269
BsaXI ACNNNNNCTCC 6 cut(s) 2237, 2267, 4746, 4776, 4877, 4907
Bsc4I CCNNNNNNNGG 9 cut(s) 252, 316, 1490, 3006, 3206, 3718, 4384, 4771, 4877
Bse118I RCCGGY 2 cut(s) 2269, 3199
Bse21I CCTNAGG 1 cut(s) 1710
Bse3DI GCAATG 4 cut(s) 231, 1095, 2107, 5193
Bse8I GATNNNNATC 2 cut(s) 63, 3681
BseBI CCWGG 9 cut(s) 212, 535, 650, 1826, 2190, 4328, 5168, 5234, 5292
BseCI ATCGAT 3 cut(s) 2943, 2985, 3685
BseJI GATNNNNATC 2 cut(s) 63, 3681
BseLI CCNNNNNNNGG 9 cut(s) 252, 316, 1490, 3006, 3206, 3718, 4384, 4771, 4877
BseMI GCAATG 4 cut(s) 231, 1095, 2107, 5193
BseRI GAGGAG 6 cut(s) 510, 513, 712, 3527, 4055, 4283
BseSI GKGCMC 5 cut(s) 247, 271, 823, 1843, 2359
BseYI CCCAGC 2 cut(s) 1460, 1892
BsgI GTGCAG 2 cut(s) 3723, 5144
Bsh1236I CGCG 1 cut(s) 2224
BshNI GGYRCC 8 cut(s) 266, 302, 1343, 2066, 2123, 2141, 2180, 4434
BshTI ACCGGT 1 cut(s) 2269
BshVI ATCGAT 3 cut(s) 2943, 2985, 3685
BsiHKAI GWGCWC 8 cut(s) 410, 764, 823, 2359, 2604, 2723, 2768, 3035
BsiSI CCGG 9 cut(s) 317, 1338, 1504, 2091, 2256, 2270, 2302, 2845, 3200
BslFI GGGAC 6 cut(s) 503, 1506, 2431, 2492, 3084, 4951
BslI CCNNNNNNNGG 9 cut(s) 252, 316, 1490, 3006, 3206, 3718, 4384, 4771, 4877
BsmBI CGTCTC 1 cut(s) 2213
BsmFI GGGAC 6 cut(s) 503, 1506, 2431, 2492, 3084, 4951
Bso31I GGTCTC 2 cut(s) 1699, 1907
Bsp119I TTCGAA 2 cut(s) 259, 4530
Bsp1407I TGTACA 2 cut(s) 1644, 3972
Bsp1720I GCTNAGC 1 cut(s) 4803
Bsp19I CCATGG 2 cut(s) 847, 3000
BspACI CCGC 8 cut(s) 563, 608, 779, 1762, 2094, 2184, 2776, 4627
BspDI ATCGAT 3 cut(s) 2943, 2985, 3685
BspFNI CGCG 1 cut(s) 2224
BspMAI CTGCAG 6 cut(s) 479, 1729, 2152, 2215, 2646, 4246
BspMI ACCTGC 4 cut(s) 1070, 1666, 2141, 3797
BspOI GCTAGC 1 cut(s) 1164
BspT104I TTCGAA 2 cut(s) 259, 4530
BspT107I GGYRCC 8 cut(s) 266, 302, 1343, 2066, 2123, 2141, 2180, 4434
BspTI CTTAAG 1 cut(s) 830
BspTNI GGTCTC 2 cut(s) 1699, 1907
BsrBI CCGCTC 1 cut(s) 2186
BsrDI GCAATG 4 cut(s) 231, 1095, 2107, 5193
BsrFI RCCGGY 2 cut(s) 2269, 3199
BsrGI TGTACA 2 cut(s) 1644, 3972
BssAI RCCGGY 2 cut(s) 2269, 3199
BssNAI GTATAC 2 cut(s) 2760, 3048
BssNI GRCGYC 1 cut(s) 2142
BssSI CACGAG 3 cut(s) 2352, 3215, 5300
BssT1I CCWWGG 5 cut(s) 379, 847, 2413, 3000, 3420
Bst1107I GTATAC 2 cut(s) 2760, 3048
Bst2BI CACGAG 3 cut(s) 2352, 3215, 5300
Bst2UI CCWGG 9 cut(s) 212, 535, 650, 1826, 2190, 4328, 5168, 5234, 5292
Bst6I CTCTTC 1 cut(s) 1554
BstACI GRCGYC 1 cut(s) 2142
BstAFI CTTAAG 1 cut(s) 830
BstAPI GCANNNNNTGC 2 cut(s) 354, 4541
BstAUI TGTACA 2 cut(s) 1644, 3972
BstBI TTCGAA 2 cut(s) 259, 4530
BstDSI CCRYGG 3 cut(s) 847, 3000, 3190
BstFNI CGCG 1 cut(s) 2224
BstH2I RGCGCY 1 cut(s) 2145
BstHHI GCGC 2 cut(s) 2144, 4140
BstNI CCWGG 9 cut(s) 212, 535, 650, 1826, 2190, 4328, 5168, 5234, 5292
BstNSI RCATGY 7 cut(s) 932, 1258, 1322, 1613, 1641, 2573, 5123
BstSFI CTRYAG 7 cut(s) 475, 1725, 2148, 2211, 2448, 2642, 4242
BstSLI GKGCMC 5 cut(s) 247, 271, 823, 1843, 2359
BstUI CGCG 1 cut(s) 2224
BstV2I GAAGAC 5 cut(s) 954, 1400, 4461, 4647, 4794
BstXI CCANNNNNNTGG 3 cut(s) 1283, 3687, 4925
BstZ17I GTATAC 2 cut(s) 2760, 3048
Bsu15I ATCGAT 3 cut(s) 2943, 2985, 3685
Bsu36I CCTNAGG 1 cut(s) 1710
BsuTUI ATCGAT 3 cut(s) 2943, 2985, 3685
BtgI CCRYGG 3 cut(s) 847, 3000, 3190
BtsI GCAGTG 2 cut(s) 1743, 3618
BveI ACCTGC 4 cut(s) 1070, 1666, 2141, 3797
CaiI CAGNNNCTG 2 cut(s) 277, 5255
CfoI GCGC 2 cut(s) 2144, 4140
Cfr10I RCCGGY 2 cut(s) 2269, 3199
Cfr13I GGNCC 8 cut(s) 137, 652, 844, 1126, 2506, 3356, 4211, 5065
ClaI ATCGAT 3 cut(s) 2943, 2985, 3685
CseI GACGC 3 cut(s) 2230, 2463, 2469
CsiI ACCWGGT 2 cut(s) 4326, 5290
Csp6I GTAC 9 cut(s) 503, 628, 1344, 1645, 1966, 3973, 4435, 4760, 5191
CspAI ACCGGT 1 cut(s) 2269
CspCI CAANNNNNGTGG 4 cut(s) 410, 445, 2312, 2347
CviQI GTAC 9 cut(s) 503, 628, 1344, 1645, 1966, 3973, 4435, 4760, 5191
DinI GGCGCC 1 cut(s) 2143
DraI TTTAAA 4 cut(s) 100, 3984, 4897, 5269
DraIII CACNNNGTG 1 cut(s) 2848
DriI GACNNNNNGTC 1 cut(s) 4835
EaeI YGGCCR 5 cut(s) 376, 1039, 1183, 1291, 2997
Eam1104I CTCTTC 1 cut(s) 1554
Eam1105I GACNNNNNGTC 1 cut(s) 4835
EarI CTCTTC 1 cut(s) 1554
Eco130I CCWWGG 5 cut(s) 379, 847, 2413, 3000, 3420
Eco31I GGTCTC 2 cut(s) 1699, 1907
Eco32I GATATC 2 cut(s) 3252, 4321
Eco47I GGWCC 5 cut(s) 137, 652, 1126, 2506, 4211
Eco57I CTGAAG 9 cut(s) 948, 1130, 2013, 3672, 4170, 4424, 4641, 4977, 5232
Eco81I CCTNAGG 1 cut(s) 1710
EcoO109I RGGNCCY 3 cut(s) 652, 1126, 3356
EcoRII CCWGG 9 cut(s) 210, 533, 648, 1824, 2188, 4326, 5166, 5232, 5290
EcoRV GATATC 2 cut(s) 3252, 4321
EcoT14I CCWWGG 5 cut(s) 379, 847, 2413, 3000, 3420
EcoT22I ATGCAT 4 cut(s) 1282, 1320, 1336, 3835
EgeI GGCGCC 1 cut(s) 2143
EheI GGCGCC 1 cut(s) 2143
ErhI CCWWGG 5 cut(s) 379, 847, 2413, 3000, 3420
Esp3I CGTCTC 1 cut(s) 2213
FaqI GGGAC 6 cut(s) 503, 1506, 2431, 2492, 3084, 4951
FauI CCCGC 1 cut(s) 4620
FauNDI CATATG 1 cut(s) 3259
FblI GTMKAC 5 cut(s) 1581, 2451, 2759, 3047, 5243
GlaI GCGC 2 cut(s) 2143, 4139
GsaI CCCAGC 2 cut(s) 1464, 1896
GsuI CTGGAG 3 cut(s) 677, 2172, 4622
HaeII RGCGCY 1 cut(s) 2145
HapII CCGG 9 cut(s) 317, 1338, 1504, 2091, 2256, 2270, 2302, 2845, 3200
HgaI GACGC 3 cut(s) 2230, 2463, 2469
HhaI GCGC 2 cut(s) 2144, 4140
Hin1I GRCGYC 1 cut(s) 2142
Hin6I GCGC 2 cut(s) 2142, 4138
HinP1I GCGC 2 cut(s) 2142, 4138
HincII GTYRAC 3 cut(s) 2296, 3927, 3952
HindII GTYRAC 3 cut(s) 2296, 3927, 3952
HindIII AAGCTT 1 cut(s) 911
HpaII CCGG 9 cut(s) 317, 1338, 1504, 2091, 2256, 2270, 2302, 2845, 3200
HphI GGTGA 9 cut(s) 581, 699, 1477, 2051, 2284, 2301, 2591, 3353, 5249
Hpy99I CGWCG 3 cut(s) 2286, 2289, 5232
HpyCH4IV ACGT 2 cut(s) 3168, 5047
HpySE526I ACGT 2 cut(s) 3168, 5047
Hsp92I GRCGYC 1 cut(s) 2142
HspAI GCGC 2 cut(s) 2142, 4138
KasI GGCGCC 1 cut(s) 2141
KpnI GGTACC 2 cut(s) 1347, 4438
LmnI GCTCC 8 cut(s) 413, 1429, 2191, 2283, 3210, 3514, 4380, 4885
MabI ACCWGGT 2 cut(s) 4326, 5290
MaeII ACGT 2 cut(s) 3168, 5047
MbiI CCGCTC 1 cut(s) 2186
MfeI CAATTG 1 cut(s) 1226
MlsI TGGCCA 4 cut(s) 378, 1185, 1293, 2999
MluNI TGGCCA 4 cut(s) 378, 1185, 1293, 2999
Mly113I GGCGCC 1 cut(s) 2142
MmeI TCCRAC 6 cut(s) 2097, 2524, 3331, 3849, 4026, 4917
Mox20I TGGCCA 4 cut(s) 378, 1185, 1293, 2999
Mph1103I ATGCAT 4 cut(s) 1282, 1320, 1336, 3835
MroXI GAANNNNTTC 4 cut(s) 3657, 4114, 4156, 4425
MscI TGGCCA 4 cut(s) 378, 1185, 1293, 2999
MslI CAYNNNNRTG 2 cut(s) 732, 4923
Msp20I TGGCCA 4 cut(s) 378, 1185, 1293, 2999
MspA1I CMGCKG 4 cut(s) 1460, 1762, 2776, 4247
MspCI CTTAAG 1 cut(s) 830
MspI CCGG 9 cut(s) 317, 1338, 1504, 2091, 2256, 2270, 2302, 2845, 3200
MunI CAATTG 1 cut(s) 1226
MvaI CCWGG 9 cut(s) 212, 535, 650, 1826, 2190, 4328, 5168, 5234, 5292
MvnI CGCG 1 cut(s) 2224
NarI GGCGCC 1 cut(s) 2142
NciI CCSGG 1 cut(s) 2846
NcoI CCATGG 2 cut(s) 847, 3000
NdeI CATATG 1 cut(s) 3259
NheI GCTAGC 1 cut(s) 1160
NmeAIII GCCGAG 2 cut(s) 3097, 3881
NmuCI GTSAC 9 cut(s) 160, 736, 1250, 2039, 2930, 3227, 3328, 5251, 5302
NsiI ATGCAT 4 cut(s) 1282, 1320, 1336, 3835
NspI RCATGY 7 cut(s) 932, 1258, 1322, 1613, 1641, 2573, 5123
NspV TTCGAA 2 cut(s) 259, 4530
PaeI GCATGC 2 cut(s) 932, 1322
PaqCI CACCTGC 3 cut(s) 1070, 1666, 3797
PciI ACATGT 3 cut(s) 1609, 1637, 2569
PcsI WCGNNNNNNNCGW 1 cut(s) 5044
PdmI GAANNNNTTC 4 cut(s) 3657, 4114, 4156, 4425
PflMI CCANNNNNTGG 3 cut(s) 3006, 4771, 4877
PfoI TCCNGGA 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 2269
PluTI GGCGCC 1 cut(s) 2145
PpuMI RGGWCCY 2 cut(s) 652, 1126
PscI ACATGT 3 cut(s) 1609, 1637, 2569
PshBI ATTAAT 3 cut(s) 2679, 3770, 3962
PsiI TTATAA 2 cut(s) 3668, 5217
Psp5II RGGWCCY 2 cut(s) 652, 1126
Psp6I CCWGG 9 cut(s) 210, 533, 648, 1824, 2188, 4326, 5166, 5232, 5290
PspFI CCCAGC 2 cut(s) 1460, 1892
PspGI CCWGG 9 cut(s) 210, 533, 648, 1824, 2188, 4326, 5166, 5232, 5290
PspPI GGNCC 8 cut(s) 137, 652, 844, 1126, 2506, 3356, 4211, 5065
PspPPI RGGWCCY 2 cut(s) 652, 1126
PstI CTGCAG 6 cut(s) 479, 1729, 2152, 2215, 2646, 4246
PstNI CAGNNNCTG 2 cut(s) 277, 5255
PvuII CAGCTG 2 cut(s) 1460, 4247
RsaI GTAC 9 cut(s) 504, 629, 1345, 1646, 1967, 3974, 4436, 4761, 5192
RsaNI GTAC 9 cut(s) 503, 628, 1344, 1645, 1966, 3973, 4435, 4760, 5191
RseI CAYNNNNRTG 2 cut(s) 732, 4923
Sau96I GGNCC 8 cut(s) 137, 652, 844, 1126, 2506, 3356, 4211, 5065
SbfI CCTGCAGG 1 cut(s) 2152
SdaI CCTGCAGG 1 cut(s) 2152
SexAI ACCWGGT 2 cut(s) 4326, 5290
SfcI CTRYAG 7 cut(s) 475, 1725, 2148, 2211, 2448, 2642, 4242
SfoI GGCGCC 1 cut(s) 2143
SfuI TTCGAA 2 cut(s) 259, 4530
SgrAI CRCCGGYG 1 cut(s) 2269
SinI GGWCC 5 cut(s) 137, 652, 1126, 2506, 4211
SmiMI CAYNNNNRTG 2 cut(s) 732, 4923
SmlI CTYRAG 6 cut(s) 336, 830, 1887, 2668, 3567, 4265
SmoI CTYRAG 6 cut(s) 336, 830, 1887, 2668, 3567, 4265
SpeI ACTAGT 1 cut(s) 157
SphI GCATGC 2 cut(s) 932, 1322
Sse8387I CCTGCAGG 1 cut(s) 2152
SsiI CCGC 8 cut(s) 563, 608, 779, 1762, 2094, 2184, 2776, 4627
SspDI GGCGCC 1 cut(s) 2141
SspI AATATT 5 cut(s) 2536, 4219, 4611, 4811, 5100
StyI CCWWGG 5 cut(s) 379, 847, 2413, 3000, 3420
TaiI ACGT 2 cut(s) 3171, 5050
TaqII GACCGA 1 cut(s) 2494
TatI WGTACW 2 cut(s) 1644, 3972
TauI GCSGC 3 cut(s) 565, 610, 2186
TseFI GTSAC 9 cut(s) 160, 736, 1250, 2039, 2930, 3227, 3328, 5251, 5302
Tsp45I GTSAC 9 cut(s) 160, 736, 1250, 2039, 2930, 3227, 3328, 5251, 5302
TspGWI ACGGA 4 cut(s) 530, 806, 2270, 3780
Van91I CCANNNNNTGG 3 cut(s) 3006, 4771, 4877
Vha464I CTTAAG 1 cut(s) 830
VneI GTGCAC 2 cut(s) 819, 2355
VpaK11BI GGWCC 5 cut(s) 137, 652, 1126, 2506, 4211
VspI ATTAAT 3 cut(s) 2679, 3770, 3962
XceI RCATGY 7 cut(s) 932, 1258, 1322, 1613, 1641, 2573, 5123
XcmI CCANNNNNNNNNTGG 1 cut(s) 3505
XmiI GTMKAC 5 cut(s) 1581, 2451, 2759, 3047, 5243
XmnI GAANNNNTTC 4 cut(s) 3657, 4114, 4156, 4425
Zsp2I ATGCAT 4 cut(s) 1282, 1320, 1336, 3835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.