pycom10g20230

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012085
Physical Location & Seq
Forward (+)
23181624 .. 23182496
873 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGCTTCACTTACTCAACAACTGGTTCCATCACTATAAGAAGCAACAACGTACTCGCTTGTGTATCTATCTATCTCAATCAACCATGGCTCTAGAAAATGCGCAGAAACTCGAGGACGGGACAGAATTAGTGGCGGTGAGAAAGCCGATGGACTGGACTATTGTCGTACTGAGGGTGGTTGCGTTTTTGGCCACGGTTTCTGCAACCATTGTGATGGCAATCAACAAAGAAACCAAGTCCATTGTGGTTGCCACTGTTGGGACTACTCCGATAAGCGTCACTCTCACAGCCAAGTTTCATCACACTCCTGCAAATGTGTACTTTGTGATAGCTAATGGAATTGCCAGCTTTCATAACTTGTTCATGATGGCTGTGCACTTTTTTGGAGACCATACGTTCAAGGACAAGGGCCTTCAACTTGTAATCGTGGCCCTTTTAGACATGATGATTGTGGCCCTATTATCTTCAGGCGATGGTGCAGCAACCTTCATGGCACAGCTAGGGAAGAAGGGAAATTCGCACGCAAGGTGGAACAAAATATGTGACAAGTTCGAGAAGTTCTGCGACCATGCCGGTGGTGCCATCATCGCTTCCTACATCGGGCTCCTTCTCTTGTTGGTTATCACAGTGTTGTCCATCGTCAAACTACTCAAAAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

221

Amino Acids

24.38

Weight (kDa)

9.59

Isoelectric Point (pI)

22.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 48 - 203 7.8e-38 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20390 AT5G44550
fragaria_vesca FvH4_3g11410
malus_domestica MD05G1263600.v1.1 MD10G1241300.v1.1
prunus_persica Prupe.4G101900_v2.0.a1
pyrus_communis pycom05g24760 pycom10g20230
rosa_chinensis RchiOBHm_Chr5g0018351
rosa_laevigata RLG00000032354
rosa_multiflora Rmu_sc0019610.1_g000001 Rmu_sc0029003.1_g000002
rosa_roxburghii Rroxscaffold_1G00059390
rosa_rugosa Rorug05G0041700.1
rosa_samantha Rh5AG135000 Rh5BG133600 Rh5CG145400 Rh5DG135000
rosa_wichuraiana Rw5G011790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 578
AciI CCGC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 514
AcuI CTGAAG 1 cut(s) 450
AfaI GTAC 3 cut(s) 52, 168, 320
AfiI CCNNNNNNNGG 2 cut(s) 258, 600
AgsI TTSAA 2 cut(s) 400, 416
AluBI AGCT 3 cut(s) 332, 348, 499
AluI AGCT 3 cut(s) 332, 348, 499
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 381
Alw44I GTGCAC 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 4 cut(s) 189, 409, 429, 453
ApaLI GTGCAC 1 cut(s) 374
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 514
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 3 cut(s) 409, 430, 454
AsuHPI GGTGA 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 378
BalI TGGCCA 1 cut(s) 191
BanI GGYRCC 1 cut(s) 578
BanII GRGCYC 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 491
BccI CCATC 7 cut(s) 36, 142, 208, 361, 467, 590, 644
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 2 cut(s) 92, 500
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 3 cut(s) 409, 430, 454
BmiI GGNNCC 3 cut(s) 26, 580, 605
BoxI GACNNNNGTC 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 84, 192
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 600
Bse118I RCCGGY 1 cut(s) 572
Bse1I ACTGG 2 cut(s) 26, 158
Bse8I GATNNNNATC 1 cut(s) 218
BseDI CCNNGG 2 cut(s) 84, 192
BseJI GATNNNNATC 1 cut(s) 218
BseLI CCNNNNNNNGG 2 cut(s) 258, 600
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 2 cut(s) 26, 158
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 1 cut(s) 491
BsgI GTGCAG 1 cut(s) 498
BshFI GGCC 4 cut(s) 191, 411, 431, 455
BshNI GGYRCC 1 cut(s) 578
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 2 cut(s) 133, 274
BslI CCNNNNNNNGG 2 cut(s) 258, 600
BsmAI GTCTC 1 cut(s) 381
BsmFI GGGAC 2 cut(s) 133, 274
BsnI GGCC 4 cut(s) 191, 411, 431, 455
Bso31I GGTCTC 1 cut(s) 381
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 2 cut(s) 378, 606
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 1 cut(s) 134
BspANI GGCC 4 cut(s) 191, 411, 431, 455
BspCNI CTCAG 1 cut(s) 162
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 3 cut(s) 26, 580, 605
BspT107I GGYRCC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 572
BsrI ACTGG 2 cut(s) 26, 158
BssAI RCCGGY 1 cut(s) 572
BssECI CCNNGG 2 cut(s) 84, 192
BssT1I CCWWGG 1 cut(s) 84
Bst4CI ACNGT 3 cut(s) 196, 256, 628
BstC8I GCNNGC 2 cut(s) 346, 522
BstDEI CTNAG 1 cut(s) 170
BstDSI CCRYGG 2 cut(s) 84, 192
BstHHI GCGC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 381
BstMWI GCNNNNNNNGC 3 cut(s) 188, 578, 587
BstPAI GACNNNNGTC 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 491
BstXI CCANNNNNNTGG 2 cut(s) 214, 575
BsuRI GGCC 4 cut(s) 191, 411, 431, 455
BtgI CCRYGG 2 cut(s) 84, 192
BtgZI GCGATG 2 cut(s) 486, 571
BtsIMutI CAGTG 2 cut(s) 252, 633
Cac8I GCNNGC 2 cut(s) 346, 522
CciI TCATGA 1 cut(s) 363
CfoI GCGC 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 572
Cfr13I GGNCC 3 cut(s) 409, 430, 454
CseI GACGC 1 cut(s) 265
Csp6I GTAC 3 cut(s) 51, 167, 319
CviAII CATG 5 cut(s) 85, 364, 442, 490, 569
CviQI GTAC 3 cut(s) 51, 167, 319
DdeI CTNAG 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 606
Eco31I GGTCTC 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 5 cut(s) 88, 367, 445, 493, 572
FaiI YATR 9 cut(s) 36, 86, 354, 365, 393, 443, 491, 541, 570
FaqI GGGAC 2 cut(s) 133, 274
FatI CATG 5 cut(s) 84, 363, 441, 489, 568
Fnu4HI GCNGC 1 cut(s) 480
FriOI GRGCYC 1 cut(s) 606
Fsp4HI GCNGC 1 cut(s) 480
FspBI CTAG 2 cut(s) 92, 500
FspI TGCGCA 1 cut(s) 102
GlaI GCGC 1 cut(s) 102
GluI GCNGC 1 cut(s) 480
HaeIII GGCC 4 cut(s) 191, 411, 431, 455
HapII CCGG 1 cut(s) 573
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 103
Hin1II CATG 5 cut(s) 88, 367, 445, 493, 572
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HpaII CCGG 1 cut(s) 573
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 2 cut(s) 319, 376
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 3 cut(s) 92, 364, 553
Hpy8I GTNNAC 2 cut(s) 319, 376
HpyAV CCTTC 4 cut(s) 422, 496, 502, 617
HpyCH4III ACNGT 3 cut(s) 196, 256, 628
HpyCH4IV ACGT 2 cut(s) 49, 395
HpyCH4V TGCA 4 cut(s) 203, 311, 376, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 578, 587
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 2 cut(s) 49, 395
Hsp92II CATG 5 cut(s) 88, 367, 445, 493, 572
HspAI GCGC 1 cut(s) 101
LmnI GCTCC 1 cut(s) 609
LpnPI CCDG 6 cut(s) 7, 139, 321, 358, 453, 586
Lsp1109I GCAGC 1 cut(s) 491
MaeI CTAG 2 cut(s) 92, 500
MaeII ACGT 2 cut(s) 49, 395
MaeIII GTNAC 2 cut(s) 277, 542
MboII GAAGA 2 cut(s) 456, 517
MhlI GDGCHC 2 cut(s) 378, 606
MlsI TGGCCA 1 cut(s) 191
MluCI AATT 4 cut(s) 125, 339, 514, 658
MluNI TGGCCA 1 cut(s) 191
MnlI CCTC 2 cut(s) 106, 165
Mox20I TGGCCA 1 cut(s) 191
MscI TGGCCA 1 cut(s) 191
MslI CAYNNNNRTG 2 cut(s) 212, 573
Msp20I TGGCCA 1 cut(s) 191
MspI CCGG 1 cut(s) 573
MwoI GCNNNNNNNGC 3 cut(s) 188, 578, 587
NcoI CCATGG 1 cut(s) 84
NlaIII CATG 5 cut(s) 88, 367, 445, 493, 572
NlaIV GGNNCC 3 cut(s) 26, 580, 605
NmuCI GTSAC 2 cut(s) 277, 542
NsbI TGCGCA 1 cut(s) 102
PaeR7I CTCGAG 1 cut(s) 110
PagI TCATGA 1 cut(s) 363
PkrI GCNGC 1 cut(s) 481
PshAI GACNNNNGTC 1 cut(s) 161
PspN4I GGNNCC 3 cut(s) 26, 580, 605
PspPI GGNCC 3 cut(s) 409, 430, 454
PspXI VCTCGAGB 1 cut(s) 110
RsaI GTAC 3 cut(s) 52, 168, 320
RsaNI GTAC 3 cut(s) 51, 167, 319
RseI CAYNNNNRTG 2 cut(s) 212, 573
SatI GCNGC 1 cut(s) 480
Sau96I GGNCC 3 cut(s) 409, 430, 454
SduI GDGCHC 2 cut(s) 378, 606
SetI ASST 7 cut(s) 52, 334, 350, 398, 488, 501, 530
Sfr274I CTCGAG 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 212, 573
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 125, 339, 514, 658
SsiI CCGC 1 cut(s) 134
SspMI CTAG 2 cut(s) 92, 500
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 3 cut(s) 196, 256, 628
TaiI ACGT 2 cut(s) 52, 398
TaqI TCGA 2 cut(s) 111, 552
TasI AATT 4 cut(s) 125, 339, 514, 658
TatI WGTACW 1 cut(s) 318
TscAI CASTG 2 cut(s) 259, 633
TseFI GTSAC 2 cut(s) 277, 542
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 2 cut(s) 277, 542
TspDTI ATGAA 4 cut(s) 287, 341, 352, 478
TspRI CASTG 2 cut(s) 259, 633
VneI GTGCAC 1 cut(s) 374
XapI RAATTY 1 cut(s) 514
XbaI TCTAGA 1 cut(s) 91
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 2 cut(s) 92, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.