pycom10g20380

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013478
Physical Location & Seq
Forward (+)
23277001 .. 23279302
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20380.1

Sequence Viewer

Length: 891 bp
ATGGACGTTCTCTCAATCTCATCCCCTATATTCCACCCCACCCCAACCTCCAAGCTCTCTCAGTCCTCCCGCCCCAAACCCACCTCCTACTTCCCCACTTCTTCCCCCAAAACCCCCTCAATCAAATCTCTTTCCCTCCAAACCCCAAAACCCACTTTCACCTCCAGGTCCCAGGCCCTCGACGGCTCGCTCCTTATTTCCTCGGCTCCTCAGACGCCGGCCACCGCCATGAGAGGCGCCGAGGGTGACGCCATGGGGCTTTTGCTCAGGGAGAGGATTGTGTTCTTGGGTAGTAGCATTGACGACTTTGTGGCCGACGCTATTATTAGCCAGCTGCTTTTGTTGGATGCCCAGGATTCTAAGAAGGATATCAAGCTCTTCATTAACTCCACCGGCGGCTCTCTCAGTGCTACAATGGCTATCTACGACGTTGTTCAGCTCGTGAGGGCTGATGTCTCCACAGTGGCACTGGGCATTGCAGCATCAACAGCGTCCATAATCCTTGGCGGTGGAACCAAGGGCAAGCGTTTTGCAATGCCGAATACACGGATCATGATTCATCAACCTCTTGGAGGCGCTAGTGGGCAGGCAATAGATGTGGAAATTCAAGCCCGAGAAGTGATGCATAACAAAAACAACGTTACACAAATTCTTTCAAATTCCACCGGTCGCCCATTTGAGCAAGTCCAAAAGGATATTGACAGGGATCGGTACATGTCCCCCATAGAAGCAGTTGAATATGGAATAATCGATGGAGTAATTGACGGAGATAGCATTATTCCTCTGGTGCCTGTCCCAGACAAGGTGAAGCCGCGAATGAGTTACGAGGAAATTAGTAAAGATCCAAGAAAATTTTTGACACCAGACGTCCCCGACGACGAGATATACTAG

Protein Analysis

297

Amino Acids

31.89

Weight (kDa)

5.69

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 83 - 255 4.1e-72 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013478)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45390
fragaria_vesca FvH4_3g11210 FvH4_3g11210
malus_domestica MD05G1265700.v1.1
prunus_persica Prupe.4G099800_v2.0.a1
pyrus_communis pycom05g24980 pycom10g20380
rosa_chinensis RchiOBHm_Chr5g0018161
rosa_laevigata RLG00000032342
rosa_multiflora Rmu_sc0006594.1_g000017 Rmu_sc0013020.1_g000003
rosa_roxburghii Rroxscaffold_1G00059490
rosa_rugosa Rorug05G0040800
rosa_samantha Rh5AG133700 Rh5BG132200 Rh5CG144400 Rh5DG134100
rosa_wichuraiana Rw5G011680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 870
AccB1I GGYRCC 2 cut(s) 236, 787
AccII CGCG 1 cut(s) 814
AciI CCGC 5 cut(s) 70, 225, 396, 507, 812
AclI AACGTT 1 cut(s) 639
AclWI GGATC 3 cut(s) 557, 714, 836
AcoI YGGCCR 2 cut(s) 219, 312
AcsI RAATTY 4 cut(s) 603, 648, 658, 851
AcyI GRCGYC 4 cut(s) 215, 237, 249, 867
AfaI GTAC 1 cut(s) 713
AfiI CCNNNNNNNGG 2 cut(s) 572, 802
AflIII ACRYGT 1 cut(s) 714
AgeI ACCGGT 1 cut(s) 665
AgsI TTSAA 3 cut(s) 608, 657, 737
AjnI CCWGG 3 cut(s) 164, 171, 351
AluBI AGCT 4 cut(s) 55, 334, 376, 439
AluI AGCT 4 cut(s) 55, 334, 376, 439
Alw26I GTCTC 1 cut(s) 460
AlwI GGATC 3 cut(s) 557, 714, 836
Ama87I CYCGRG 1 cut(s) 612
AoxI GGCC 3 cut(s) 174, 219, 312
ApeKI GCWGC 2 cut(s) 334, 479
ApoI RAATTY 4 cut(s) 603, 648, 658, 851
AsiGI ACCGGT 1 cut(s) 665
AspLEI GCGC 2 cut(s) 239, 578
AspS9I GGNCC 2 cut(s) 168, 175
AsuHPI GGTGA 3 cut(s) 151, 257, 817
AvaI CYCGRG 1 cut(s) 612
AvaII GGWCC 1 cut(s) 168
BanI GGYRCC 2 cut(s) 236, 787
BauI CACGAG 1 cut(s) 440
BbvI GCAGC 2 cut(s) 321, 491
BccI CCATC 1 cut(s) 746
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 3 cut(s) 166, 173, 353
BcoDI GTCTC 1 cut(s) 460
BfaI CTAG 2 cut(s) 579, 889
BfoI RGCGCY 2 cut(s) 240, 579
BisI GCNGC 4 cut(s) 335, 397, 480, 812
BlsI GCNGC 4 cut(s) 336, 398, 481, 813
Bme1390I CCNGG 3 cut(s) 166, 173, 353
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 612
BmgT120I GGNCC 2 cut(s) 168, 175
BmiI GGNNCC 5 cut(s) 170, 207, 238, 514, 789
BmrFI CCNGG 3 cut(s) 166, 173, 353
BmrI ACTGGG 1 cut(s) 479
BmsI GCATC 3 cut(s) 337, 491, 612
BmuI ACTGGG 1 cut(s) 479
BpmI CTGGAG 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 266
Bsa29I ATCGAT 1 cut(s) 750
BsaHI GRCGYC 4 cut(s) 215, 237, 249, 867
BsaJI CCNNGG 7 cut(s) 171, 201, 240, 252, 351, 502, 516
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 802
Bse118I RCCGGY 3 cut(s) 217, 392, 665
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 2 cut(s) 474, 540
BseBI CCWGG 3 cut(s) 166, 173, 353
BseCI ATCGAT 1 cut(s) 750
BseDI CCNNGG 7 cut(s) 171, 201, 240, 252, 351, 502, 516
BseGI GGATG 2 cut(s) 20, 352
BseLI CCNNNNNNNGG 2 cut(s) 572, 802
BseMI GCAATG 2 cut(s) 474, 540
BseMII CTCAG 4 cut(s) 74, 224, 280, 418
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 2 cut(s) 321, 491
Bsh1236I CGCG 1 cut(s) 814
Bsh1285I CGRYCG 1 cut(s) 670
BshFI GGCC 3 cut(s) 176, 221, 314
BshNI GGYRCC 2 cut(s) 236, 787
BshTI ACCGGT 1 cut(s) 665
BshVI ATCGAT 1 cut(s) 750
BsiEI CGRYCG 1 cut(s) 670
BsiHKCI CYCGRG 1 cut(s) 612
BsiSI CCGG 3 cut(s) 218, 393, 666
BslFI GGGAC 4 cut(s) 154, 703, 779, 854
BslI CCNNNNNNNGG 2 cut(s) 572, 802
BsmAI GTCTC 1 cut(s) 460
BsmFI GGGAC 4 cut(s) 154, 703, 779, 854
BsnI GGCC 3 cut(s) 176, 221, 314
BsoBI CYCGRG 1 cut(s) 612
Bsp143I GATC 3 cut(s) 549, 706, 841
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 5 cut(s) 70, 225, 396, 507, 812
BspANI GGCC 3 cut(s) 176, 221, 314
BspCNI CTCAG 4 cut(s) 73, 223, 279, 417
BspDI ATCGAT 1 cut(s) 750
BspFNI CGCG 1 cut(s) 814
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 5 cut(s) 170, 207, 238, 514, 789
BspPI GGATC 3 cut(s) 557, 714, 836
BspQI GCTCTTC 1 cut(s) 383
BspT107I GGYRCC 2 cut(s) 236, 787
BsrDI GCAATG 2 cut(s) 474, 540
BsrFI RCCGGY 3 cut(s) 217, 392, 665
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 3 cut(s) 217, 392, 665
BssECI CCNNGG 7 cut(s) 171, 201, 240, 252, 351, 502, 516
BssMI GATC 3 cut(s) 549, 706, 841
BssNI GRCGYC 4 cut(s) 215, 237, 249, 867
BssSI CACGAG 1 cut(s) 440
BssT1I CCWWGG 3 cut(s) 252, 502, 516
Bst2BI CACGAG 1 cut(s) 440
Bst2UI CCWGG 3 cut(s) 166, 173, 353
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 1 cut(s) 383
BstACI GRCGYC 4 cut(s) 215, 237, 249, 867
BstC8I GCNNGC 5 cut(s) 188, 219, 332, 524, 588
BstDEI CTNAG 5 cut(s) 60, 210, 266, 360, 404
BstDSI CCRYGG 1 cut(s) 252
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 2 cut(s) 20, 352
BstFNI CGCG 1 cut(s) 814
BstH2I RGCGCY 2 cut(s) 240, 579
BstHHI GCGC 2 cut(s) 239, 578
BstKTI GATC 3 cut(s) 552, 709, 844
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 3 cut(s) 549, 706, 841
BstMCI CGRYCG 1 cut(s) 670
BstMWI GCNNNNNNNGC 2 cut(s) 416, 488
BstNI CCWGG 3 cut(s) 166, 173, 353
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 3 cut(s) 164, 171, 351
BstUI CGCG 1 cut(s) 814
BstV1I GCAGC 2 cut(s) 321, 491
BstX2I RGATCY 1 cut(s) 841
BstYI RGATCY 1 cut(s) 841
Bsu15I ATCGAT 1 cut(s) 750
BsuRI GGCC 3 cut(s) 176, 221, 314
BsuTUI ATCGAT 1 cut(s) 750
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 2 cut(s) 20, 352
BtsIMutI CAGTG 3 cut(s) 412, 467, 468
Cac8I GCNNGC 5 cut(s) 188, 219, 332, 524, 588
CciI TCATGA 1 cut(s) 552
CfoI GCGC 2 cut(s) 239, 578
Cfr10I RCCGGY 3 cut(s) 217, 392, 665
Cfr13I GGNCC 2 cut(s) 168, 175
ClaI ATCGAT 1 cut(s) 750
CseI GACGC 4 cut(s) 223, 257, 326, 480
Csp6I GTAC 1 cut(s) 712
CspAI ACCGGT 1 cut(s) 665
CspCI CAANNNNNGTGG 2 cut(s) 579, 614
CviAII CATG 4 cut(s) 229, 253, 553, 715
CviQI GTAC 1 cut(s) 712
DdeI CTNAG 5 cut(s) 60, 210, 266, 360, 404
DinI GGCGCC 1 cut(s) 238
DpnI GATC 3 cut(s) 551, 708, 843
DpnII GATC 3 cut(s) 549, 706, 841
EaeI YGGCCR 2 cut(s) 219, 312
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
Eco130I CCWWGG 3 cut(s) 252, 502, 516
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 612
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoO109I RGGNCCY 2 cut(s) 168, 175
EcoRII CCWGG 3 cut(s) 164, 171, 351
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 3 cut(s) 252, 502, 516
EcoT22I ATGCAT 1 cut(s) 627
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 3 cut(s) 252, 502, 516
FaeI CATG 4 cut(s) 232, 256, 556, 718
FaqI GGGAC 4 cut(s) 154, 703, 779, 854
FatI CATG 4 cut(s) 228, 252, 552, 714
FauI CCCGC 1 cut(s) 77
Fnu4HI GCNGC 4 cut(s) 335, 397, 480, 812
FokI GGATG 2 cut(s) 7, 359
Fsp4HI GCNGC 4 cut(s) 335, 397, 480, 812
FspBI CTAG 2 cut(s) 579, 889
GlaI GCGC 2 cut(s) 238, 577
GluI GCNGC 4 cut(s) 335, 397, 480, 812
GsuI CTGGAG 1 cut(s) 148
HaeII RGCGCY 2 cut(s) 240, 579
HaeIII GGCC 3 cut(s) 176, 221, 314
HapII CCGG 3 cut(s) 218, 393, 666
HgaI GACGC 4 cut(s) 223, 257, 326, 480
HhaI GCGC 2 cut(s) 239, 578
Hin1I GRCGYC 4 cut(s) 215, 237, 249, 867
Hin1II CATG 4 cut(s) 232, 256, 556, 718
Hin6I GCGC 2 cut(s) 237, 576
HinP1I GCGC 2 cut(s) 237, 576
HinfI GANTC 2 cut(s) 356, 556
HpaII CCGG 3 cut(s) 218, 393, 666
HphI GGTGA 3 cut(s) 151, 257, 817
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 2 cut(s) 442, 553
Hpy99I CGWCG 5 cut(s) 185, 320, 431, 878, 881
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4IV ACGT 4 cut(s) 6, 429, 639, 867
HpyCH4V TGCA 3 cut(s) 479, 533, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 488
HpyF3I CTNAG 5 cut(s) 60, 210, 266, 360, 404
HpySE526I ACGT 4 cut(s) 6, 429, 639, 867
Hsp92I GRCGYC 4 cut(s) 215, 237, 249, 867
Hsp92II CATG 4 cut(s) 232, 256, 556, 718
HspAI GCGC 2 cut(s) 237, 576
KasI GGCGCC 1 cut(s) 236
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
Kzo9I GATC 3 cut(s) 549, 706, 841
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 2 cut(s) 195, 211
Lsp1109I GCAGC 2 cut(s) 321, 491
LweI GCATC 3 cut(s) 337, 491, 612
MaeI CTAG 2 cut(s) 579, 889
MaeII ACGT 4 cut(s) 6, 429, 639, 867
MaeIII GTNAC 3 cut(s) 245, 640, 821
MalI GATC 3 cut(s) 551, 708, 843
MboI GATC 3 cut(s) 549, 706, 841
MboII GAAGA 2 cut(s) 93, 370
MflI RGATCY 1 cut(s) 841
MluCI AATT 6 cut(s) 603, 648, 658, 759, 831, 851
Mly113I GGCGCC 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 324
Mph1103I ATGCAT 1 cut(s) 627
MroNI GCCGGC 1 cut(s) 217
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 3 cut(s) 218, 393, 666
MspR9I CCNGG 3 cut(s) 166, 173, 353
MvaI CCWGG 3 cut(s) 166, 173, 353
MvnI CGCG 1 cut(s) 814
MwoI GCNNNNNNNGC 2 cut(s) 416, 488
NaeI GCCGGC 1 cut(s) 219
NarI GGCGCC 1 cut(s) 237
NcoI CCATGG 1 cut(s) 252
NdeII GATC 3 cut(s) 549, 706, 841
NgoMIV GCCGGC 1 cut(s) 217
NlaIII CATG 4 cut(s) 232, 256, 556, 718
NlaIV GGNNCC 5 cut(s) 170, 207, 238, 514, 789
NmeAIII GCCGAG 2 cut(s) 182, 265
NmuCI GTSAC 1 cut(s) 245
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 1 cut(s) 718
PagI TCATGA 1 cut(s) 552
PciI ACATGT 1 cut(s) 714
PciSI GCTCTTC 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 873
PdiI GCCGGC 1 cut(s) 219
PfeI GAWTC 2 cut(s) 356, 556
PinAI ACCGGT 1 cut(s) 665
PkrI GCNGC 4 cut(s) 336, 398, 481, 813
PluTI GGCGCC 1 cut(s) 240
PpuMI RGGWCCY 1 cut(s) 168
PscI ACATGT 1 cut(s) 714
Psp1406I AACGTT 1 cut(s) 639
Psp5II RGGWCCY 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 164, 171, 351
PspGI CCWGG 3 cut(s) 164, 171, 351
PspN4I GGNNCC 5 cut(s) 170, 207, 238, 514, 789
PspPI GGNCC 2 cut(s) 168, 175
PspPPI RGGWCCY 1 cut(s) 168
PsuI RGATCY 1 cut(s) 841
PvuII CAGCTG 1 cut(s) 334
RsaI GTAC 1 cut(s) 713
RsaNI GTAC 1 cut(s) 712
RseI CAYNNNNRTG 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 4 cut(s) 335, 397, 480, 812
Sau3AI GATC 3 cut(s) 549, 706, 841
Sau96I GGNCC 2 cut(s) 168, 175
ScrFI CCNGG 3 cut(s) 166, 173, 353
SfaNI GCATC 3 cut(s) 337, 491, 612
SfoI GGCGCC 1 cut(s) 238
SgrAI CRCCGGYG 1 cut(s) 392
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 6 cut(s) 603, 648, 658, 759, 831, 851
SsiI CCGC 5 cut(s) 70, 225, 396, 507, 812
SspDI GGCGCC 1 cut(s) 236
SspMI CTAG 2 cut(s) 579, 889
StyD4I CCNGG 3 cut(s) 164, 171, 351
StyI CCWWGG 3 cut(s) 252, 502, 516
TaaI ACNGT 1 cut(s) 463
TaiI ACGT 4 cut(s) 9, 432, 642, 870
TaqI TCGA 2 cut(s) 180, 750
TasI AATT 6 cut(s) 603, 648, 658, 759, 831, 851
TauI GCSGC 2 cut(s) 399, 814
TfiI GAWTC 2 cut(s) 356, 556
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 3 cut(s) 412, 468, 474
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 334, 479
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 370, 548
TspGWI ACGGA 2 cut(s) 562, 780
TspRI CASTG 3 cut(s) 412, 468, 474
VpaK11BI GGWCC 1 cut(s) 168
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 4 cut(s) 603, 648, 658, 851
XceI RCATGY 1 cut(s) 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XspI CTAG 2 cut(s) 579, 889
ZraI GACGTC 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.