Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0008814

Physical Location & Seq

Genomic Coordinates Reverse (-)

25416496 .. 25419095

Gene Structure 2600 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g23200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2082 bp

ATGGGAACCCTGTTGAGATTTTCACTTCTTCTGGTGCTGATTGCAGCAGCTGAAGCAGAATCCACCTGCAACAGCACAGACCAAGCCCAGGTCTCCAAGGCTTTCAAGTCAGTCTCCGGTTTCAACCTCCCCTGGCTCCAAAATTCAAACCGACCCAACTGCTCCAACCCACCCATCACTCAAATCAACTTCCCCTCCAGAAACCTAACCGGAATAATCTCATGGAAGTACCTCAGAAACTTGTCAAATCTTCAATCCCTCGATCTCTCATCCAACAATCTCAAGGGCTCCGTCCCTGGATGGTTTTGGACTCTTCCGAACCTGGTCCGGGTAAATCTCTCTAAGAACCGGCTCGGAGGCAGCTTTGGACTCGAACCCGCGTCAGGAGACAGTTCAACCTCTTCGGTCCAGGAGCTCAATCTCTCATCCAACCGGTTCACCAACTCGGCCCAGCTCTCTGGCTTCCCAAAGCTTACATTTCTCGACCTTTCCCACAACGATTTGGGCACTCTGCAACCATCTGCTTTTTCCAACCTCACTAATTTGCAGCACCTCGACATCTCGAGCTGCAGAATTGCTGGGAATTTAAAACCCATCTCTGTTTTGAGAGGATTGGAGTATTTAGACGTTTCGGACAACAAAATCAACGGTTCTTTCCCTTCGGATTTTCCTCCGCTTACCGGCCTCAAATTCTTGAACATTTCGCTCAATAATTTTACCGGCCGGGTTGCACTGGGAAATTACCTGAAGTTTGGGAAAAGCGCTTTTATCCACGCCGGAAAAAACCTCACTTCTTTCGGCAGTTCCAAAACCAAAGCCCAAAGCTTTCACACTTCCAATGCAAACCCACCTCACAACACCCTCGAAAAACAAAAACCCATGACCCAAAATTCCCCCCACAAAAAACCCAAATCGCAAAAATCCCATAAAATTCTTGTTCTGAGCATATCCTGCGCATCCGCATTTGTAATCCTCTTGTCCATAAGCACTTGCGTAGCTTGCATATGCAGGAAAAAGCACTTTGCCAAAAAGCACAAATGGGCGATTTCCAAACCGGTTCAGTTAGTCCCGTTCAAGATCGAGAAGTCGGGGCCGTTCTCTTTCGAGACCGAGTCGGGGACGTCGTGGGTCGCTGACATCAAGGAGCCGACGTCGGCTCCGGTCGTGATGTTCGAGAAGCCGCTGATGAACTTGACGTTTAAGGACTTGATTGCTGCCACGTCGCACTTCGGTAAGGACTCCCAGCTTGCAGAGGGGAGGTGTGGGCCCGTCTACACCGCCGTGCTGCCCGGCGATCTCCACGTGGCAATCAAGGTGTTGGAGAACGCCAGGGATGTTGAGTACGATGATGCGGTGGAAGTGTTTGAAAATCTTTCGAGATTGAAACATCCCAACCTGCTGCCTCTCTCCGGTTACTGCATTGCAGGGAGAGAGAAGTTGATATTGTACGAGTTCATGGCGAACGGGGACTTGCACAGATGGTTGCACGAGCTGCCCACCGGGGAGCCCAACGTGGAGGACTGGACTGGTGACATGTGGGAGCAAAGCTTTGGTTATGGAGCCCACTCGCCCGAAAAGAAAGGATGGCTCACGCGCCACCGCATTGCGGTTGGAATTGCGCGTGGGTTGGCGTACCTCCACCAGGCAGGGTCGAGGCCCGTTGTCCACGGCCACCTTGTAACTTCGAACATATTGTTGGGCGGGGATTTTGAGCCGAGAATAGCCGATTTTGGGTTGAAAAGTATTGGGAGTAGGCGGGAAATTGGCGGCGGAGGTGGTGGCAGCGGGGCGGAGACGGATGTGTATTGTTTTGGGGTCGTGTTGATGGAGCTGTTGACGGGGAGGATGGGGACGGCGGAGACGGTGGTGTGGGTGAGGAGGATGGTGAGGGAGGGGCGTGCGGTGGACGCACTGGACGAGAAGTTGAGAGAGAGTGGGGAGTTGGAGAGGGAGATGGTGGAGAGCTTGCGAGTTGGGTACTTGTGTACGGCGGAGTCGCCTGGGAAAAGGCCCAGCATGCAACAAGTTCTGGGTTTGCTCAAAGACATACATCCCAGTACCACTACTGAGGGCTTCGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005516 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009625 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010154 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0061458 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_4 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

694

Amino Acids

75.88

Weight (kDa)

9.04

Isoelectric Point (pI)

36.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	68 - 118	3.5e-09	Leucine rich repeat
LRR_8	PF13855	135 - 192	1.7e-09	Leucine rich repeat
LRR_4	PF12799	156 - 192	1.1e-06	Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr	PF07714	413 - 581	2.7e-21	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	413 - 677	5.7e-14	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45800 AT5G45800
fragaria_vesca	FvH4_3g07560 FvH4_3g07560
malus_domestica	MD05G1298700.v1.1 MD10G1278000.v1.1
prunus_persica	Prupe.4G063700_v2.0.a1
pyrus_communis	pycom05g27650 pycom10g23200
rosa_chinensis	RchiOBHm_Chr5g0011151
rosa_laevigata	RLG00000031815
rosa_multiflora	Rmu_co8285897.1_g000001 Rmu_sc0002990.1_g000016 Rmu_sc0003242.1_g000021 Rmu_sc0030140.1_g000001
rosa_roxburghii	Rroxscaffold_1G00065240
rosa_rugosa	Rorug04G0455400
rosa_samantha	Rh5AG087700 Rh5BG082400 Rh5CG095400 Rh5DG082200
rosa_wichuraiana	Rw0G000210 Rw5G007580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	74
AatII	GACGTC	2 cut(s)	1124, 1154
Acc16I	TGCGCA	1 cut(s)	955
Acc36I	ACCTGC	2 cut(s)	74, 1404
AccI	GTMKAC	1 cut(s)	1272
AccII	CGCG	3 cut(s)	380, 1594, 1621
AcoI	YGGCCR	2 cut(s)	721, 1669
AcsI	RAATTY	5 cut(s)	142, 583, 689, 889, 930
AcuI	CTGAAG	2 cut(s)	72, 767
AcvI	CACGTG	1 cut(s)	1303
AcyI	GRCGYC	2 cut(s)	1121, 1151
AfaI	GTAC	7 cut(s)	230, 1343, 1448, 1634, 1979, 1987, 2059
AfeI	AGCGCT	1 cut(s)	763
AfiI	CCNNNNNNNGG	9 cut(s)	88, 328, 383, 680, 1116, 1409, 1606, 1642, 1731
AflIII	ACRYGT	1 cut(s)	1533
AgeI	ACCGGT	2 cut(s)	432, 1054
AjiI	CACGTC	1 cut(s)	1221
AjnI	CCWGG	8 cut(s)	87, 131, 295, 321, 408, 1328, 1641, 1999
AjuI	GAANNNNNNNTTGG	2 cut(s)	1679, 1711
AleI	CACNNNNGTG	1 cut(s)	1280
Alw21I	GWGCWC	1 cut(s)	417
Alw26I	GTCTC	6 cut(s)	97, 118, 381, 1100, 1787, 1853
AlwNI	CAGNNNCTG	1 cut(s)	50
Ama87I	CYCGRG	1 cut(s)	562
Aor51HI	AGCGCT	1 cut(s)	763
AoxI	GGCC	8 cut(s)	447, 682, 721, 1091, 1265, 1655, 1669, 2009
ApaI	GGGCCC	1 cut(s)	1269
ApoI	RAATTY	5 cut(s)	142, 583, 689, 889, 930
AsiGI	ACCGGT	2 cut(s)	432, 1054
AspLEI	GCGC	4 cut(s)	764, 956, 1596, 1621
AspS9I	GGNCC	8 cut(s)	325, 406, 448, 1091, 1265, 1266, 1656, 2010
AsuC2I	CCSGG	4 cut(s)	329, 725, 1290, 1501
AsuHPI	GGTGA	4 cut(s)	430, 1541, 1885, 1897
AsuII	TTCGAA	1 cut(s)	1685
AvaI	CYCGRG	1 cut(s)	562
AvaII	GGWCC	2 cut(s)	325, 406
BaeGI	GKGCMC	2 cut(s)	509, 1269
BaeI	ACNNNNGTAYC	2 cut(s)	1969, 2002
BanII	GRGCYC	5 cut(s)	290, 417, 1269, 1509, 1564
BauI	CACGAG	1 cut(s)	1487
BbrPI	CACGTG	1 cut(s)	1303
Bbv12I	GWGCWC	1 cut(s)	417
BceAI	ACGGC	5 cut(s)	1078, 1265, 1684, 1869, 2004
BcgI	CGANNNNNNTGC	2 cut(s)	141, 175
BciT130I	CCWGG	8 cut(s)	89, 133, 297, 323, 410, 1330, 1643, 2001
BcnI	CCSGG	4 cut(s)	329, 725, 1290, 1501
BcoDI	GTCTC	6 cut(s)	97, 118, 381, 1100, 1787, 1853
BfmI	CTRYAG	1 cut(s)	568
BfoI	RGCGCY	1 cut(s)	765
BfuAI	ACCTGC	2 cut(s)	74, 1404
Bme18I	GGWCC	2 cut(s)	325, 406
BmeT110I	CYCGRG	1 cut(s)	562
BmgBI	CACGTC	1 cut(s)	1221
BmgT120I	GGNCC	8 cut(s)	325, 406, 448, 1091, 1265, 1266, 1656, 2010
BmiI	GGNNCC	9 cut(s)	7, 137, 289, 1092, 1146, 1158, 1267, 1506, 1561
BmrI	ACTGGG	2 cut(s)	743, 2049
BmsI	GCATC	2 cut(s)	965, 1339
BmuI	ACTGGG	2 cut(s)	743, 2049
BpmI	CTGGAG	1 cut(s)	181
Bpu14I	TTCGAA	1 cut(s)	1685
BpuEI	CTTGAG	1 cut(s)	266
BpuMI	CCSGG	4 cut(s)	329, 725, 1290, 1501
BsaAI	YACGTR	1 cut(s)	1303
BsaHI	GRCGYC	2 cut(s)	1121, 1151
BsaI	GGTCTC	2 cut(s)	97, 1100
BsaJI	CCNNGG	8 cut(s)	87, 96, 131, 295, 1329, 1500, 1666, 2000
BsaWI	WCCGGW	6 cut(s)	116, 209, 432, 1054, 1159, 1409
BsaXI	ACNNNNNCTCC	6 cut(s)	179, 209, 1136, 1141, 1166, 1171
Bsc4I	CCNNNNNNNGG	9 cut(s)	88, 328, 383, 680, 1116, 1409, 1606, 1642, 1731
Bse118I	RCCGGY	5 cut(s)	348, 432, 680, 719, 1054
Bse1I	ACTGG	5 cut(s)	738, 1526, 1531, 1917, 2055
Bse3DI	GCAATG	2 cut(s)	1419, 1602
BseBI	CCWGG	8 cut(s)	89, 133, 297, 323, 410, 1330, 1643, 2001
BseDI	CCNNGG	8 cut(s)	87, 96, 131, 295, 1329, 1500, 1666, 2000
BseLI	CCNNNNNNNGG	9 cut(s)	88, 328, 383, 680, 1116, 1409, 1606, 1642, 1731
BseMI	GCAATG	2 cut(s)	1419, 1602
BseMII	CTCAG	3 cut(s)	247, 932, 2058
BseNI	ACTGG	5 cut(s)	738, 1526, 1531, 1917, 2055
BseRI	GAGGAG	1 cut(s)	1891
BseSI	GKGCMC	2 cut(s)	509, 1269
BseX3I	CGGCCG	1 cut(s)	721
BseYI	CCCAGC	4 cut(s)	450, 578, 1242, 2012
Bsh1236I	CGCG	3 cut(s)	380, 1594, 1621
Bsh1285I	CGRYCG	2 cut(s)	724, 1164
BshFI	GGCC	8 cut(s)	449, 684, 723, 1093, 1267, 1657, 1671, 2011
BshTI	ACCGGT	2 cut(s)	432, 1054
BsiEI	CGRYCG	2 cut(s)	724, 1164
BsiHKAI	GWGCWC	1 cut(s)	417
BsiHKCI	CYCGRG	1 cut(s)	562
BslFI	GGGAC	5 cut(s)	278, 1052, 1132, 1481, 1864
BslI	CCNNNNNNNGG	9 cut(s)	88, 328, 383, 680, 1116, 1409, 1606, 1642, 1731
BsmAI	GTCTC	6 cut(s)	97, 118, 381, 1100, 1787, 1853
BsmBI	CGTCTC	2 cut(s)	1787, 1853
BsmFI	GGGAC	5 cut(s)	278, 1052, 1132, 1481, 1864
BsnI	GGCC	8 cut(s)	449, 684, 723, 1093, 1267, 1657, 1671, 2011
Bso31I	GGTCTC	2 cut(s)	97, 1100
BsoBI	CYCGRG	1 cut(s)	562
Bsp119I	TTCGAA	1 cut(s)	1685
Bsp120I	GGGCCC	1 cut(s)	1265
Bsp1286I	GDGCHC	6 cut(s)	290, 417, 509, 1269, 1509, 1564
Bsp143I	GATC	3 cut(s)	262, 1077, 1294
BspANI	GGCC	8 cut(s)	449, 684, 723, 1093, 1267, 1657, 1671, 2011
BspCNI	CTCAG	3 cut(s)	246, 933, 2059
BspFNI	CGCG	3 cut(s)	380, 1594, 1621
BspLI	GGNNCC	9 cut(s)	7, 137, 289, 1092, 1146, 1158, 1267, 1506, 1561
BspMAI	CTGCAG	1 cut(s)	572
BspMI	ACCTGC	2 cut(s)	74, 1404
BspT104I	TTCGAA	1 cut(s)	1685
BspTNI	GGTCTC	2 cut(s)	97, 1100
BsrDI	GCAATG	2 cut(s)	1419, 1602
BsrFI	RCCGGY	5 cut(s)	348, 432, 680, 719, 1054
BsrI	ACTGG	5 cut(s)	738, 1526, 1531, 1917, 2055
BssAI	RCCGGY	5 cut(s)	348, 432, 680, 719, 1054
BssECI	CCNNGG	8 cut(s)	87, 96, 131, 295, 1329, 1500, 1666, 2000
BssMI	GATC	3 cut(s)	262, 1077, 1294
BssNI	GRCGYC	2 cut(s)	1121, 1151
BssSI	CACGAG	1 cut(s)	1487
BssT1I	CCWWGG	1 cut(s)	96
Bst2BI	CACGAG	1 cut(s)	1487
Bst2UI	CCWGG	8 cut(s)	89, 133, 297, 323, 410, 1330, 1643, 2001
Bst4CI	ACNGT	3 cut(s)	392, 650, 1864
Bst6I	CTCTTC	2 cut(s)	318, 406
BstACI	GRCGYC	2 cut(s)	1121, 1151
BstAPI	GCANNNNNTGC	2 cut(s)	951, 1492
BstBAI	YACGTR	1 cut(s)	1303
BstBI	TTCGAA	1 cut(s)	1685
BstC8I	GCNNGC	5 cut(s)	1000, 1248, 1899, 1967, 2018
BstDEI	CTNAG	4 cut(s)	233, 342, 941, 2067
BstDSI	CCRYGG	1 cut(s)	1666
BstENI	CCTNNNNNAGG	1 cut(s)	1640
BstFNI	CGCG	3 cut(s)	380, 1594, 1621
BstH2I	RGCGCY	1 cut(s)	765
BstHHI	GCGC	4 cut(s)	764, 956, 1596, 1621
BstKTI	GATC	3 cut(s)	265, 1080, 1297
BstMAI	GTCTC	6 cut(s)	97, 118, 381, 1100, 1787, 1853
BstMBI	GATC	3 cut(s)	262, 1077, 1294
BstMCI	CGRYCG	2 cut(s)	724, 1164
BstMWI	GCNNNNNNNGC	6 cut(s)	53, 951, 999, 1492, 1907, 2017
BstNI	CCWGG	8 cut(s)	89, 133, 297, 323, 410, 1330, 1643, 2001
BstNSI	RCATGY	2 cut(s)	1537, 2020
BstSFI	CTRYAG	1 cut(s)	568
BstSLI	GKGCMC	2 cut(s)	509, 1269
BstUI	CGCG	3 cut(s)	380, 1594, 1621
BstXI	CCANNNNNNTGG	1 cut(s)	458
BstZI	CGGCCG	1 cut(s)	721
BsuRI	GGCC	8 cut(s)	449, 684, 723, 1093, 1267, 1657, 1671, 2011
BtgI	CCRYGG	1 cut(s)	1666
BtrI	CACGTC	1 cut(s)	1221
BtsIMutI	CAGTG	2 cut(s)	731, 1910
BveI	ACCTGC	2 cut(s)	74, 1404
Cac8I	GCNNGC	5 cut(s)	1000, 1248, 1899, 1967, 2018
CaiI	CAGNNNCTG	1 cut(s)	50
CfoI	GCGC	4 cut(s)	764, 956, 1596, 1621
Cfr10I	RCCGGY	5 cut(s)	348, 432, 680, 719, 1054
Cfr13I	GGNCC	8 cut(s)	325, 406, 448, 1091, 1265, 1266, 1656, 2010
CseI	GACGC	2 cut(s)	369, 1916
CsiI	ACCWGGT	1 cut(s)	321
Csp6I	GTAC	7 cut(s)	229, 1342, 1447, 1633, 1978, 1986, 2058
CspAI	ACCGGT	2 cut(s)	432, 1054
CviAII	CATG	5 cut(s)	222, 880, 1456, 1534, 2017
CviQI	GTAC	7 cut(s)	229, 1342, 1447, 1633, 1978, 1986, 2058
DdeI	CTNAG	4 cut(s)	233, 342, 941, 2067
DpnI	GATC	3 cut(s)	264, 1079, 1296
DpnII	GATC	3 cut(s)	262, 1077, 1294
DraI	TTTAAA	1 cut(s)	588
EaeI	YGGCCR	2 cut(s)	721, 1669
EagI	CGGCCG	1 cut(s)	721
Eam1104I	CTCTTC	2 cut(s)	318, 406
EarI	CTCTTC	2 cut(s)	318, 406
EciI	GGCGGA	4 cut(s)	1785, 1805, 1871, 2006
Ecl136II	GAGCTC	1 cut(s)	415
EclXI	CGGCCG	1 cut(s)	721
Eco130I	CCWWGG	1 cut(s)	96
Eco24I	GRGCYC	5 cut(s)	290, 417, 1269, 1509, 1564
Eco31I	GGTCTC	2 cut(s)	97, 1100
Eco47I	GGWCC	2 cut(s)	325, 406
Eco47III	AGCGCT	1 cut(s)	763
Eco52I	CGGCCG	1 cut(s)	721
Eco53kI	GAGCTC	1 cut(s)	415
Eco57I	CTGAAG	2 cut(s)	72, 767
Eco72I	CACGTG	1 cut(s)	1303
Eco88I	CYCGRG	1 cut(s)	562
EcoICRI	GAGCTC	1 cut(s)	415
EcoNI	CCTNNNNNAGG	1 cut(s)	1640
EcoRII	CCWGG	8 cut(s)	87, 131, 295, 321, 408, 1328, 1641, 1999
EcoT14I	CCWWGG	1 cut(s)	96
EcoT38I	GRGCYC	5 cut(s)	290, 417, 1269, 1509, 1564
ErhI	CCWWGG	1 cut(s)	96
Esp3I	CGTCTC	2 cut(s)	1787, 1853
FaeI	CATG	5 cut(s)	225, 883, 1459, 1537, 2020
FaqI	GGGAC	5 cut(s)	278, 1052, 1132, 1481, 1864
FatI	CATG	5 cut(s)	221, 879, 1455, 1533, 2016
FauI	CCCGC	4 cut(s)	385, 1694, 1749, 1778
FauNDI	CATATG	1 cut(s)	1004
FblI	GTMKAC	1 cut(s)	1272
FriOI	GRGCYC	5 cut(s)	290, 417, 1269, 1509, 1564
FspI	TGCGCA	1 cut(s)	955
GlaI	GCGC	4 cut(s)	763, 955, 1595, 1620
GsaI	CCCAGC	4 cut(s)	454, 582, 1246, 2016
GsuI	CTGGAG	1 cut(s)	181
HaeII	RGCGCY	1 cut(s)	765
HaeIII	GGCC	8 cut(s)	449, 684, 723, 1093, 1267, 1657, 1671, 2011
HgaI	GACGC	2 cut(s)	369, 1916
HhaI	GCGC	4 cut(s)	764, 956, 1596, 1621
Hin1I	GRCGYC	2 cut(s)	1121, 1151
Hin1II	CATG	5 cut(s)	225, 883, 1459, 1537, 2020
Hin6I	GCGC	4 cut(s)	762, 954, 1594, 1619
HinP1I	GCGC	4 cut(s)	762, 954, 1594, 1619
HincII	GTYRAC	1 cut(s)	1836
HindII	GTYRAC	1 cut(s)	1836
HindIII	AAGCTT	3 cut(s)	470, 823, 1546
HinfI	GANTC	6 cut(s)	59, 310, 369, 1112, 1238, 1994
HphI	GGTGA	4 cut(s)	430, 1541, 1885, 1897
Hpy166II	GTNNAC	6 cut(s)	438, 1273, 1666, 1836, 1906, 1986
Hpy188I	TCNGA	6 cut(s)	236, 318, 356, 634, 664, 942
Hpy8I	GTNNAC	6 cut(s)	438, 1273, 1666, 1836, 1906, 1986
Hpy99I	CGWCG	4 cut(s)	1126, 1153, 1156, 1225
HpyAV	CCTTC	1 cut(s)	669
HpyCH4III	ACNGT	3 cut(s)	392, 650, 1864
HpyCH4IV	ACGT	7 cut(s)	627, 1121, 1151, 1196, 1220, 1302, 1512
HpyF10VI	GCNNNNNNNGC	6 cut(s)	53, 951, 999, 1492, 1907, 2017
HpyF3I	CTNAG	4 cut(s)	233, 342, 941, 2067
HpySE526I	ACGT	7 cut(s)	627, 1121, 1151, 1196, 1220, 1302, 1512
Hsp92I	GRCGYC	2 cut(s)	1121, 1151
Hsp92II	CATG	5 cut(s)	225, 883, 1459, 1537, 2020
HspAI	GCGC	4 cut(s)	762, 954, 1594, 1619
Kzo9I	GATC	3 cut(s)	262, 1077, 1294
LweI	GCATC	2 cut(s)	965, 1339
MabI	ACCWGGT	1 cut(s)	321
MaeII	ACGT	7 cut(s)	627, 1121, 1151, 1196, 1220, 1302, 1512
MaeIII	GTNAC	3 cut(s)	1412, 1529, 1678
MalI	GATC	3 cut(s)	264, 1079, 1296
MboI	GATC	3 cut(s)	262, 1077, 1294
MboII	GAAGA	4 cut(s)	20, 242, 305, 393
MhlI	GDGCHC	6 cut(s)	290, 417, 509, 1269, 1509, 1564
MlyI	GAGTC	5 cut(s)	304, 363, 1121, 1232, 2003
MmeI	TCCRAC	7 cut(s)	189, 297, 453, 555, 1299, 1591, 1923
MseI	TTAA	2 cut(s)	587, 1200
MslI	CAYNNNNRTG	1 cut(s)	1280
MspA1I	CMGCKG	3 cut(s)	50, 1183, 1785
MvaI	CCWGG	8 cut(s)	89, 133, 297, 323, 410, 1330, 1643, 2001
MvnI	CGCG	3 cut(s)	380, 1594, 1621
MwoI	GCNNNNNNNGC	6 cut(s)	53, 951, 999, 1492, 1907, 2017
NciI	CCSGG	4 cut(s)	329, 725, 1290, 1501
NdeI	CATATG	1 cut(s)	1004
NdeII	GATC	3 cut(s)	262, 1077, 1294
NlaIII	CATG	5 cut(s)	225, 883, 1459, 1537, 2020
NlaIV	GGNNCC	9 cut(s)	7, 137, 289, 1092, 1146, 1158, 1267, 1506, 1561
NmeAIII	GCCGAG	2 cut(s)	425, 1740
NmuCI	GTSAC	1 cut(s)	1529
NsbI	TGCGCA	1 cut(s)	955
NspI	RCATGY	2 cut(s)	1537, 2020
NspV	TTCGAA	1 cut(s)	1685
OliI	CACNNNNGTG	1 cut(s)	1280
PaeI	GCATGC	1 cut(s)	2020
PaeR7I	CTCGAG	1 cut(s)	562
PaqCI	CACCTGC	1 cut(s)	74
PciI	ACATGT	1 cut(s)	1533
PcsI	WCGNNNNNNNCGW	3 cut(s)	1121, 1170, 1914
PfeI	GAWTC	1 cut(s)	59
PflFI	GACNNNGTC	1 cut(s)	1111
PfoI	TCCNGGA	1 cut(s)	408
PinAI	ACCGGT	2 cut(s)	432, 1054
PleI	GAGTC	5 cut(s)	304, 363, 1120, 1232, 2002
PmaCI	CACGTG	1 cut(s)	1303
PmlI	CACGTG	1 cut(s)	1303
PpsI	GAGTC	5 cut(s)	304, 363, 1120, 1232, 2002
Ppu21I	YACGTR	1 cut(s)	1303
PscI	ACATGT	1 cut(s)	1533
Psp124BI	GAGCTC	1 cut(s)	417
Psp6I	CCWGG	8 cut(s)	87, 131, 295, 321, 408, 1328, 1641, 1999
PspCI	CACGTG	1 cut(s)	1303
PspFI	CCCAGC	4 cut(s)	450, 578, 1242, 2012
PspGI	CCWGG	8 cut(s)	87, 131, 295, 321, 408, 1328, 1641, 1999
PspN4I	GGNNCC	9 cut(s)	7, 137, 289, 1092, 1146, 1158, 1267, 1506, 1561
PspOMI	GGGCCC	1 cut(s)	1265
PspPI	GGNCC	8 cut(s)	325, 406, 448, 1091, 1265, 1266, 1656, 2010
PstI	CTGCAG	1 cut(s)	572
PstNI	CAGNNNCTG	1 cut(s)	50
PsyI	GACNNNGTC	1 cut(s)	1111
PvuII	CAGCTG	1 cut(s)	50
RsaI	GTAC	7 cut(s)	230, 1343, 1448, 1634, 1979, 1987, 2059
RsaNI	GTAC	7 cut(s)	229, 1342, 1447, 1633, 1978, 1986, 2058
RseI	CAYNNNNRTG	1 cut(s)	1280
SacI	GAGCTC	1 cut(s)	417
SaqAI	TTAA	2 cut(s)	587, 1200
Sau3AI	GATC	3 cut(s)	262, 1077, 1294
Sau96I	GGNCC	8 cut(s)	325, 406, 448, 1091, 1265, 1266, 1656, 2010
SchI	GAGTC	5 cut(s)	304, 363, 1121, 1232, 2003
SduI	GDGCHC	6 cut(s)	290, 417, 509, 1269, 1509, 1564
SexAI	ACCWGGT	1 cut(s)	321
SfaNI	GCATC	2 cut(s)	965, 1339
SfcI	CTRYAG	1 cut(s)	568
Sfr274I	CTCGAG	1 cut(s)	562
SfuI	TTCGAA	1 cut(s)	1685
SinI	GGWCC	2 cut(s)	325, 406
SlaI	CTCGAG	1 cut(s)	562
SmiMI	CAYNNNNRTG	1 cut(s)	1280
SmlI	CTYRAG	2 cut(s)	281, 562
SmoI	CTYRAG	2 cut(s)	281, 562
SphI	GCATGC	1 cut(s)	2020
SstI	GAGCTC	1 cut(s)	417
StyI	CCWWGG	1 cut(s)	96
TaaI	ACNGT	3 cut(s)	392, 650, 1864
TaiI	ACGT	7 cut(s)	630, 1124, 1154, 1199, 1223, 1305, 1515
TaqII	GACCGA	2 cut(s)	394, 1124
TauI	GCSGC	2 cut(s)	1183, 1770
TfiI	GAWTC	1 cut(s)	59
Tru1I	TTAA	2 cut(s)	587, 1200
Tru9I	TTAA	2 cut(s)	587, 1200
TscAI	CASTG	2 cut(s)	738, 1917
TseFI	GTSAC	1 cut(s)	1529
Tsp45I	GTSAC	1 cut(s)	1529
TspDTI	ATGAA	2 cut(s)	1202, 1444
TspGWI	ACGGA	2 cut(s)	280, 1811
TspRI	CASTG	2 cut(s)	738, 1917
Tth111I	GACNNNGTC	1 cut(s)	1111
VpaK11BI	GGWCC	2 cut(s)	325, 406
XagI	CCTNNNNNAGG	1 cut(s)	1640
XapI	RAATTY	5 cut(s)	142, 583, 689, 889, 930
XceI	RCATGY	2 cut(s)	1537, 2020
XhoI	CTCGAG	1 cut(s)	562
XmiI	GTMKAC	1 cut(s)	1272
ZraI	GACGTC	2 cut(s)	1122, 1152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.