Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007916

Physical Location & Seq

Genomic Coordinates Forward (+)

26251939 .. 26256967

Gene Structure 5029 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGGGTTTGCCGGAAATCGTAGATTTCGCGCGCAATTTTTCCGTCATGGTCAGAGTCAAAGGCCCTGATCCAAAGGGCCTCAAAATGCGAAACCACGCTTTCCACCATTACCACTCTGGGACGACGACCATTTCGGCGTCGGGAATGTTATTGCCGGACACCCTTTACGACTCTGGTGTTGCTAAGCAACTTTTTGGCGGCCGTGGAGACGGTGGAGGCGGTGGGGGTGGCAGCCAACGTTCTCCTGTTCTCGTGGTGACTGTTGCTTCCATTGTTGAGCCTTTTCTCTCTTTGGAGCACAGAAAGGGCCTTGCCCAGGGCAGGCATCAGTTGATTCCTGGGGTTCAGATTGATATTATGGTAGAGGATGAAATGAGGTTGGAGAAAGAATCTCGGGATTCGGATAAAGCACATCCCTGCTGGTTTGCAGCACAGCTTTTAACACTGATTGACGTTCCTGCATCTGCTGCTGCAATCCAATCACTTATTGAAGCTTCTTTAAGCTCCCCCGATCACGGATGGGAGGTCGGTTGGTCTTTAGCCTCACATAGTAATGCTCCTCAGGCCCAGGAGGACTTTGGAGTCAGAGAATTGGGGAATTTAACTCTCACAGGCAAGTCAAATACAAGAATCGCCATTCTTGGAGTATCCCTAATTCCCCAGGACGTGCCAAATATAACCATATCACCCTCCAATCAAAGGGGGGACTTTCTTGTGGCTGTGGGTTCTCCTTTTGGTATCTTGTCAACCGTGCACTTCTTTAACAGTATATCAGTGGGATCCATTGCCAACTGTTATCCTCCTAATTCATCTTCTAGTTCATTGCTGATGGCAGACATACGCTGTCTTCCTGGAGTGGAAGGGGGTCCAGTTTTGAGTGAGCAGGCACAACTTATTGGTATGTTGATTAGACCGTTGAGGCAGAAGACTAGTGGGGCTGAGATTCAGCTGGTGATTTCATGGGAAGCCATTGCAACTGCTTGCAGTGACTTGCTGCGGAAGGAGCCCCATTATGCAGAAAAAGGGATTTCTGTTGACAGGGGAACCTCAGATGATGTAGGGAAGCCAATTTTAGCTGAGAGCCACGGTTCAAATGGAGCCATAACACATATTCAGGAATGCATTTATTCTAGTTGCTCCTCCCCGTCACCAGTTGAGAAGGCAATGGGTTCTGTTTGTCTTATAAACATTGATGATGGAGCATGGGCATCAGGTGTCTTTCTAAACAAGCAAGGTCTCATACTCACAAATGCTCACCTGTTAGAACCATGGAGATTTGGTAAAAGAACTGCAAGTGATGGAAGGCATGGATCCATTTCTGAGGCATTATCTCCTAGGCATAGTGGACTATATGGCAAGCAAAAGAGTGAAGGTTGTCCCCCAATGGTTCACAATAATGCAGATCCCTCAGTTCGTGATGAACATGGTGGATATGTATTGAGTTCTTACAGAGGTCAGAGAAGGATACGTGTACGCCTAGATCATAGAGACCCTTGGATTTGGTGTGATGCTAAAGTAGTGTATGTTTGCAAGGGACCTGTGGATGTTGCCTTGTTGCAACTCAAGTACATCACTGATCAGCTTTCTCCAATTGTCATGGATTTTATGTCGCCATCTGTAGGATCAAAGGCATATGTTATTGGACATGGACTATTAGGACCGAGATGCGGGTTCTCCCCGTCAATTTGTTCTGGTGTTGTCGCAAAAGTTTTAAAGTCAAAGTTTCCGCACTCATATCAAACAAACCAACCAGGAAGTACTCAAGGAGATCTTCCTGCGATGCTTGAAACAACAGCTGCTGTCCATCCTGGTGGTAGTGGTGGCGCTGTCGTTAATTCAGAGGGTCAAATGATTGGACTTGTTACAAGTAATGCAAGGCATGGTGGAGGAACAATTATACCACATCTGAACTTCAGCATTCCATGTGCTGCTCTCCTGCCAATCTTCAAGTTCTCAAAAGACATGCAGGATCCTTCACTTCTCCAAGTTCTTGACCAACCAAACGAATATCTTTCTTCTGTATGGGCGTTGATGCCACCTGTGTCTCCCAAGCCCCCTCCTTTGCCGCCTCATATTCCAGAGTCATTACTACAAGATAACAACAAAGAAGCAAAAGGCTCTCGTTTTGCAAGATTTGTCGCCGAAAGAAAAGATGTGTTTGAAAACCCAAACCAACTTGGCAAGGAAAGAAGACTTCCTAACAACATTGTTCCTAGCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0010467 GO:0016485 GO:0016787 GO:0017171 GO:0019538 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051604 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Trypsin_2

Protein Analysis

742

Amino Acids

79.53

Weight (kDa)

7.12

Isoelectric Point (pI)

45.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	201 - 301	1e-07	Trypsin-like peptidase domain
Trypsin_2	PF13365	403 - 620	9e-17	Trypsin-like peptidase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007916)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28320
fragaria_vesca	FvH4_3g08860 FvH4_3g08860 FvH4_3g08860
malus_domestica	MD10G1291600.v1.1
prunus_persica	Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1
pyrus_communis	pycom10g24380
rosa_chinensis	RchiOBHm_Chr5g0008891 RchiOBHm_Chr5g0008901
rosa_laevigata	RLG00000022692 RLG00000031614
rosa_multiflora	Rmu_co8519179.1_g000001 Rmu_sc0004677.1_g000010
rosa_roxburghii	Rroxscaffold_1G00067110 Rroxscaffold_1G00067730
rosa_rugosa	Rorug04G0440000 Rorug04G0440000
rosa_samantha	Rh5AG068700 Rh5BG065400 Rh5CG076600 Rh5DG064400
rosa_wichuraiana	Rw5G006430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1184
AasI	GACNNNNNNGTC	1 cut(s)	581
AccII	CGCG	2 cut(s)	29, 31
AciI	CCGC	6 cut(s)	198, 219, 997, 1668, 1727, 2066
AclI	AACGTT	1 cut(s)	238
AclWI	GGATC	9 cut(s)	62, 774, 787, 1305, 1318, 1397, 1630, 1964, 1977
AcoI	YGGCCR	1 cut(s)	199
AcsI	RAATTY	1 cut(s)	598
AcuI	CTGAAG	1 cut(s)	1897
AcyI	GRCGYC	1 cut(s)	137
AfaI	GTAC	3 cut(s)	1473, 1568, 1759
AfiI	CCNNNNNNNGG	6 cut(s)	316, 321, 515, 699, 1619, 1667
AflIII	ACRYGT	1 cut(s)	1468
AgsI	TTSAA	5 cut(s)	491, 1092, 1787, 1948, 2162
AhlI	ACTAGT	1 cut(s)	929
AjiI	CACGTC	1 cut(s)	667
AjnI	CCWGG	7 cut(s)	315, 337, 567, 660, 850, 1750, 1807
AluBI	AGCT	7 cut(s)	436, 494, 504, 949, 1076, 1582, 1796
AluI	AGCT	7 cut(s)	436, 494, 504, 949, 1076, 1582, 1796
Alw21I	GWGCWC	2 cut(s)	300, 756
Alw26I	GTCTC	4 cut(s)	201, 1241, 1482, 2049
Alw44I	GTGCAC	1 cut(s)	752
AlwI	GGATC	9 cut(s)	62, 774, 787, 1305, 1318, 1397, 1630, 1964, 1977
AlwNI	CAGNNNCTG	1 cut(s)	1799
Ama87I	CYCGRG	1 cut(s)	393
AoxI	GGCC	5 cut(s)	61, 76, 199, 307, 564
ApaLI	GTGCAC	1 cut(s)	752
ApeKI	GCWGC	7 cut(s)	231, 428, 467, 470, 994, 1796, 1928
ApoI	RAATTY	1 cut(s)	598
ArsI	GACNNNNNNTTYG	2 cut(s)	115, 147
AspA2I	CCTAGG	1 cut(s)	1334
AspLEI	GCGC	3 cut(s)	31, 33, 1826
AspS9I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
AsuHPI	GGTGA	5 cut(s)	268, 678, 964, 1140, 1247
AvaI	CYCGRG	1 cut(s)	393
AvaII	GGWCC	3 cut(s)	866, 1535, 1658
AvrII	CCTAGG	1 cut(s)	1334
AxyI	CCTNAGG	1 cut(s)	561
BaeGI	GKGCMC	1 cut(s)	756
BamHI	GGATCC	3 cut(s)	779, 1310, 1969
BanII	GRGCYC	1 cut(s)	1008
BauI	CACGAG	1 cut(s)	251
BbsI	GAAGAC	3 cut(s)	839, 932, 2197
Bbv12I	GWGCWC	2 cut(s)	300, 756
BbvI	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1783, 1915
BccI	CCATC	6 cut(s)	513, 823, 1190, 1292, 1621, 1812
BceAI	ACGGC	1 cut(s)	186
BciT130I	CCWGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
BciVI	GTATCC	2 cut(s)	658, 1458
BclI	TGATCA	1 cut(s)	1576
BcoDI	GTCTC	4 cut(s)	201, 1241, 1482, 2049
BcuI	ACTAGT	1 cut(s)	929
BfaI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2214
BfmI	CTRYAG	1 cut(s)	1617
BfoI	RGCGCY	1 cut(s)	1827
BfuI	GTATCC	2 cut(s)	658, 1458
BglII	AGATCT	1 cut(s)	1768
BisI	GCNGC	9 cut(s)	199, 232, 429, 468, 471, 995, 1797, 1929, 2066
BlnI	CCTAGG	1 cut(s)	1334
BlpI	GCTNAGC	1 cut(s)	183
BlsI	GCNGC	9 cut(s)	200, 233, 430, 469, 472, 996, 1798, 1930, 2067
BmcAI	AGTACT	1 cut(s)	1759
Bme1390I	CCNGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
Bme18I	GGWCC	3 cut(s)	866, 1535, 1658
BmeT110I	CYCGRG	1 cut(s)	393
BmgBI	CACGTC	1 cut(s)	667
BmgT120I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
BmiI	GGNNCC	8 cut(s)	781, 867, 1005, 1045, 1099, 1312, 1536, 1971
BmrFI	CCNGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
BmsI	GCATC	7 cut(s)	334, 470, 1217, 1498, 1655, 1770, 2022
BpiI	GAAGAC	3 cut(s)	839, 932, 2197
BpmI	CTGGAG	1 cut(s)	873
Bpu1102I	GCTNAGC	1 cut(s)	183
BpuEI	CTTGAG	2 cut(s)	1547, 1746
BsaAI	YACGTR	1 cut(s)	1469
BsaHI	GRCGYC	1 cut(s)	137
BsaI	GGTCTC	2 cut(s)	1241, 1482
BsaXI	ACNNNNNCTCC	2 cut(s)	1264, 1294
Bsc4I	CCNNNNNNNGG	6 cut(s)	316, 321, 515, 699, 1619, 1667
Bse1I	ACTGG	2 cut(s)	869, 1151
Bse21I	CCTNAGG	1 cut(s)	561
Bse3DI	GCAATG	4 cut(s)	783, 821, 969, 1170
BseBI	CCWGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
BseGI	GGATG	5 cut(s)	373, 412, 524, 1549, 1804
BseLI	CCNNNNNNNGG	6 cut(s)	316, 321, 515, 699, 1619, 1667
BseMI	GCAATG	4 cut(s)	783, 821, 969, 1170
BseMII	CTCAG	6 cut(s)	575, 930, 1062, 1068, 1311, 1422
BseNI	ACTGG	2 cut(s)	869, 1151
BsePI	GCGCGC	1 cut(s)	29
BseRI	GAGGAG	2 cut(s)	549, 1129
BseSI	GKGCMC	1 cut(s)	756
BseX3I	CGGCCG	1 cut(s)	199
BseXI	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1783, 1915
Bsh1236I	CGCG	2 cut(s)	29, 31
Bsh1285I	CGRYCG	1 cut(s)	202
BshFI	GGCC	5 cut(s)	63, 78, 201, 309, 566
BsiEI	CGRYCG	1 cut(s)	202
BsiHKAI	GWGCWC	2 cut(s)	300, 756
BsiHKCI	CYCGRG	1 cut(s)	393
BsiSI	CCGG	2 cut(s)	11, 155
BslFI	GGGAC	4 cut(s)	133, 719, 1362, 1548
BslI	CCNNNNNNNGG	6 cut(s)	316, 321, 515, 699, 1619, 1667
BsmAI	GTCTC	4 cut(s)	201, 1241, 1482, 2049
BsmBI	CGTCTC	1 cut(s)	201
BsmFI	GGGAC	4 cut(s)	133, 719, 1362, 1548
BsmI	GAATGC	2 cut(s)	1124, 1917
BsnI	GGCC	5 cut(s)	63, 78, 201, 309, 566
Bso31I	GGTCTC	2 cut(s)	1241, 1482
BsoBI	CYCGRG	1 cut(s)	393
Bsp1286I	GDGCHC	3 cut(s)	300, 756, 1008
Bsp1720I	GCTNAGC	1 cut(s)	183
Bsp19I	CCATGG	1 cut(s)	1268
BspACI	CCGC	6 cut(s)	198, 219, 997, 1668, 1727, 2066
BspANI	GGCC	5 cut(s)	63, 78, 201, 309, 566
BspCNI	CTCAG	6 cut(s)	574, 931, 1061, 1069, 1312, 1421
BspFNI	CGCG	2 cut(s)	29, 31
BspLI	GGNNCC	8 cut(s)	781, 867, 1005, 1045, 1099, 1312, 1536, 1971
BspPI	GGATC	9 cut(s)	62, 774, 787, 1305, 1318, 1397, 1630, 1964, 1977
BspTNI	GGTCTC	2 cut(s)	1241, 1482
BsrDI	GCAATG	4 cut(s)	783, 821, 969, 1170
BsrI	ACTGG	2 cut(s)	869, 1151
BssHII	GCGCGC	1 cut(s)	29
BssNI	GRCGYC	1 cut(s)	137
BssSI	CACGAG	1 cut(s)	251
BssT1I	CCWWGG	3 cut(s)	1268, 1334, 1493
Bst2BI	CACGAG	1 cut(s)	251
Bst2UI	CCWGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
Bst4CI	ACNGT	7 cut(s)	212, 262, 751, 767, 794, 915, 1088
BstACI	GRCGYC	1 cut(s)	137
BstAPI	GCANNNNNTGC	1 cut(s)	467
BstBAI	YACGTR	1 cut(s)	1469
BstC8I	GCNNGC	5 cut(s)	31, 323, 885, 982, 1358
BstDEI	CTNAG	7 cut(s)	183, 561, 939, 1048, 1077, 1320, 1408
BstDSI	CCRYGG	3 cut(s)	202, 1084, 1268
BstENI	CCTNNNNNAGG	1 cut(s)	314
BstF5I	GGATG	5 cut(s)	373, 412, 524, 1549, 1804
BstFNI	CGCG	2 cut(s)	29, 31
BstH2I	RGCGCY	1 cut(s)	1827
BstHHI	GCGC	3 cut(s)	31, 33, 1826
BstMAI	GTCTC	4 cut(s)	201, 1241, 1482, 2049
BstMCI	CGRYCG	1 cut(s)	202
BstMWI	GCNNNNNNNGC	3 cut(s)	467, 563, 1003
BstNI	CCWGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
BstNSI	RCATGY	1 cut(s)	1966
BstSCI	CCNGG	7 cut(s)	315, 337, 567, 660, 850, 1750, 1807
BstSFI	CTRYAG	1 cut(s)	1617
BstSLI	GKGCMC	1 cut(s)	756
BstUI	CGCG	2 cut(s)	29, 31
BstV1I	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1783, 1915
BstV2I	GAAGAC	3 cut(s)	839, 932, 2197
BstX2I	RGATCY	5 cut(s)	779, 1310, 1402, 1768, 1969
BstXI	CCANNNNNNTGG	1 cut(s)	1811
BstYI	RGATCY	5 cut(s)	779, 1310, 1402, 1768, 1969
BstZI	CGGCCG	1 cut(s)	199
Bsu36I	CCTNAGG	1 cut(s)	561
BsuI	GTATCC	2 cut(s)	658, 1458
BsuRI	GGCC	5 cut(s)	63, 78, 201, 309, 566
BtgI	CCRYGG	3 cut(s)	202, 1084, 1268
BtgZI	GCGATG	1 cut(s)	1793
BtrI	CACGTC	1 cut(s)	667
BtsCI	GGATG	5 cut(s)	373, 412, 524, 1549, 1804
BtsI	GCAGTG	1 cut(s)	991
BtsIMutI	CAGTG	4 cut(s)	443, 780, 991, 1572
Cac8I	GCNNGC	5 cut(s)	31, 323, 885, 982, 1358
CaiI	CAGNNNCTG	1 cut(s)	1799
CfoI	GCGC	3 cut(s)	31, 33, 1826
Cfr13I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
CseI	GACGC	1 cut(s)	126
Csp6I	GTAC	3 cut(s)	1472, 1567, 1758
CviQI	GTAC	3 cut(s)	1472, 1567, 1758
DdeI	CTNAG	7 cut(s)	183, 561, 939, 1048, 1077, 1320, 1408
DraI	TTTAAA	1 cut(s)	1713
DrdI	GACNNNNNNGTC	1 cut(s)	581
DseDI	GACNNNNNNGTC	1 cut(s)	581
EaeI	YGGCCR	1 cut(s)	199
EagI	CGGCCG	1 cut(s)	199
EclXI	CGGCCG	1 cut(s)	199
Eco130I	CCWWGG	3 cut(s)	1268, 1334, 1493
Eco24I	GRGCYC	1 cut(s)	1008
Eco31I	GGTCTC	2 cut(s)	1241, 1482
Eco47I	GGWCC	3 cut(s)	866, 1535, 1658
Eco52I	CGGCCG	1 cut(s)	199
Eco57I	CTGAAG	1 cut(s)	1897
Eco81I	CCTNAGG	1 cut(s)	561
Eco88I	CYCGRG	1 cut(s)	393
EcoNI	CCTNNNNNAGG	1 cut(s)	314
EcoO109I	RGGNCCY	4 cut(s)	62, 76, 307, 1535
EcoRII	CCWGG	7 cut(s)	315, 337, 567, 660, 850, 1750, 1807
EcoT14I	CCWWGG	3 cut(s)	1268, 1334, 1493
EcoT22I	ATGCAT	1 cut(s)	1124
EcoT38I	GRGCYC	1 cut(s)	1008
ErhI	CCWWGG	3 cut(s)	1268, 1334, 1493
Esp3I	CGTCTC	1 cut(s)	201
FalI	AAGNNNNNCTT	2 cut(s)	1755, 1787
FaqI	GGGAC	4 cut(s)	133, 719, 1362, 1548
FauI	CCCGC	1 cut(s)	1661
FauNDI	CATATG	1 cut(s)	1633
FbaI	TGATCA	1 cut(s)	1576
Fnu4HI	GCNGC	9 cut(s)	199, 232, 429, 468, 471, 995, 1797, 1929, 2066
FokI	GGATG	5 cut(s)	380, 399, 531, 1556, 1791
FriOI	GRGCYC	1 cut(s)	1008
Fsp4HI	GCNGC	9 cut(s)	199, 232, 429, 468, 471, 995, 1797, 1929, 2066
FspBI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2214
GlaI	GCGC	3 cut(s)	30, 32, 1825
GluI	GCNGC	9 cut(s)	199, 232, 429, 468, 471, 995, 1797, 1929, 2066
GsuI	CTGGAG	1 cut(s)	873
HaeII	RGCGCY	1 cut(s)	1827
HaeIII	GGCC	5 cut(s)	63, 78, 201, 309, 566
HapII	CCGG	2 cut(s)	11, 155
HgaI	GACGC	1 cut(s)	126
HhaI	GCGC	3 cut(s)	31, 33, 1826
Hin1I	GRCGYC	1 cut(s)	137
Hin6I	GCGC	3 cut(s)	29, 31, 1824
HinP1I	GCGC	3 cut(s)	29, 31, 1824
HincII	GTYRAC	2 cut(s)	747, 1036
HindII	GTYRAC	2 cut(s)	747, 1036
HindIII	AAGCTT	1 cut(s)	492
HinfI	GANTC	9 cut(s)	54, 170, 334, 389, 398, 582, 630, 943, 2081
HpaII	CCGG	2 cut(s)	11, 155
HphI	GGTGA	5 cut(s)	268, 678, 964, 1140, 1247
Hpy166II	GTNNAC	6 cut(s)	747, 754, 1036, 1346, 1390, 1472
Hpy188I	TCNGA	9 cut(s)	53, 348, 403, 587, 1051, 1321, 1458, 1840, 1908
Hpy188III	TCNNGA	6 cut(s)	141, 395, 1115, 1415, 1991, 2078
Hpy8I	GTNNAC	6 cut(s)	747, 754, 1036, 1346, 1390, 1472
Hpy99I	CGWCG	2 cut(s)	127, 142
HpyAV	CCTTC	7 cut(s)	854, 994, 1153, 1296, 1364, 1455, 1983
HpyCH4III	ACNGT	7 cut(s)	212, 262, 751, 767, 794, 915, 1088
HpyCH4IV	ACGT	4 cut(s)	238, 453, 666, 1468
HpyF10VI	GCNNNNNNNGC	3 cut(s)	467, 563, 1003
HpyF3I	CTNAG	7 cut(s)	183, 561, 939, 1048, 1077, 1320, 1408
HpySE526I	ACGT	4 cut(s)	238, 453, 666, 1468
Hsp92I	GRCGYC	1 cut(s)	137
HspAI	GCGC	3 cut(s)	29, 31, 1824
Ksp22I	TGATCA	1 cut(s)	1576
LmnI	GCTCC	7 cut(s)	295, 509, 562, 1003, 1097, 1142, 1199
Lsp1109I	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1783, 1915
LweI	GCATC	7 cut(s)	334, 470, 1217, 1498, 1655, 1770, 2022
MaeI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2214
MaeII	ACGT	4 cut(s)	238, 453, 666, 1468
MaeIII	GTNAC	4 cut(s)	256, 986, 1146, 1861
MboII	GAAGA	7 cut(s)	804, 839, 937, 1763, 1936, 2007, 2202
MfeI	CAATTG	1 cut(s)	1590
MflI	RGATCY	5 cut(s)	779, 1310, 1402, 1768, 1969
MhlI	GDGCHC	3 cut(s)	300, 756, 1008
MlyI	GAGTC	4 cut(s)	63, 164, 591, 2090
MmeI	TCCRAC	1 cut(s)	360
Mph1103I	ATGCAT	1 cut(s)	1124
MseI	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1833
MslI	CAYNNNNRTG	3 cut(s)	552, 1395, 1809
MspA1I	CMGCKG	2 cut(s)	949, 1796
MspI	CCGG	2 cut(s)	11, 155
MspR9I	CCNGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
MunI	CAATTG	1 cut(s)	1590
Mva1269I	GAATGC	2 cut(s)	1124, 1917
MvaI	CCWGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
MvnI	CGCG	2 cut(s)	29, 31
MwoI	GCNNNNNNNGC	3 cut(s)	467, 563, 1003
NcoI	CCATGG	1 cut(s)	1268
NdeI	CATATG	1 cut(s)	1633
NlaIV	GGNNCC	8 cut(s)	781, 867, 1005, 1045, 1099, 1312, 1536, 1971
NmuCI	GTSAC	3 cut(s)	256, 986, 1146
NsiI	ATGCAT	1 cut(s)	1124
NspI	RCATGY	1 cut(s)	1966
PasI	CCCWGGG	1 cut(s)	316
PauI	GCGCGC	1 cut(s)	29
PctI	GAATGC	2 cut(s)	1124, 1917
PfeI	GAWTC	5 cut(s)	334, 389, 398, 630, 943
PfoI	TCCNGGA	1 cut(s)	850
PkrI	GCNGC	9 cut(s)	200, 233, 430, 469, 472, 996, 1798, 1930, 2067
PleI	GAGTC	4 cut(s)	62, 164, 590, 2089
PpsI	GAGTC	4 cut(s)	62, 164, 590, 2089
Ppu21I	YACGTR	1 cut(s)	1469
PpuMI	RGGWCCY	1 cut(s)	1535
PsiI	TTATAA	1 cut(s)	1184
Psp1406I	AACGTT	1 cut(s)	238
Psp5II	RGGWCCY	1 cut(s)	1535
Psp6I	CCWGG	7 cut(s)	315, 337, 567, 660, 850, 1750, 1807
PspGI	CCWGG	7 cut(s)	315, 337, 567, 660, 850, 1750, 1807
PspN4I	GGNNCC	8 cut(s)	781, 867, 1005, 1045, 1099, 1312, 1536, 1971
PspPI	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
PspPPI	RGGWCCY	1 cut(s)	1535
PstNI	CAGNNNCTG	1 cut(s)	1799
PsuI	RGATCY	5 cut(s)	779, 1310, 1402, 1768, 1969
PteI	GCGCGC	1 cut(s)	29
PvuII	CAGCTG	2 cut(s)	949, 1796
RsaI	GTAC	3 cut(s)	1473, 1568, 1759
RsaNI	GTAC	3 cut(s)	1472, 1567, 1758
RseI	CAYNNNNRTG	3 cut(s)	552, 1395, 1809
SaqAI	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1833
SatI	GCNGC	9 cut(s)	199, 232, 429, 468, 471, 995, 1797, 1929, 2066
Sau96I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
ScaI	AGTACT	1 cut(s)	1759
SchI	GAGTC	4 cut(s)	63, 164, 591, 2090
ScrFI	CCNGG	7 cut(s)	317, 339, 569, 662, 852, 1752, 1809
SduI	GDGCHC	3 cut(s)	300, 756, 1008
SfaNI	GCATC	7 cut(s)	334, 470, 1217, 1498, 1655, 1770, 2022
SfcI	CTRYAG	1 cut(s)	1617
SinI	GGWCC	3 cut(s)	866, 1535, 1658
SmiMI	CAYNNNNRTG	3 cut(s)	552, 1395, 1809
SmlI	CTYRAG	2 cut(s)	1562, 1761
SmoI	CTYRAG	2 cut(s)	1562, 1761
SpeI	ACTAGT	1 cut(s)	929
SsiI	CCGC	6 cut(s)	198, 219, 997, 1668, 1727, 2066
SspMI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2214
StyD4I	CCNGG	7 cut(s)	315, 337, 567, 660, 850, 1750, 1807
StyI	CCWWGG	3 cut(s)	1268, 1334, 1493
TaaI	ACNGT	7 cut(s)	212, 262, 751, 767, 794, 915, 1088
TaiI	ACGT	4 cut(s)	241, 456, 669, 1471
TaqII	GACCGA	1 cut(s)	1675
TatI	WGTACW	2 cut(s)	1566, 1757
TauI	GCSGC	2 cut(s)	201, 2068
TfiI	GAWTC	5 cut(s)	334, 389, 398, 630, 943
Tru1I	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1833
Tru9I	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1833
TscAI	CASTG	4 cut(s)	450, 780, 991, 1579
TseFI	GTSAC	3 cut(s)	256, 986, 1146
TseI	GCWGC	7 cut(s)	231, 428, 467, 470, 994, 1796, 1928
Tsp45I	GTSAC	3 cut(s)	256, 986, 1146
TspDTI	ATGAA	5 cut(s)	384, 798, 810, 948, 1434
TspGWI	ACGGA	2 cut(s)	31, 531
TspRI	CASTG	4 cut(s)	450, 780, 991, 1579
VneI	GTGCAC	1 cut(s)	752
VpaK11BI	GGWCC	3 cut(s)	866, 1535, 1658
XagI	CCTNNNNNAGG	1 cut(s)	314
XapI	RAATTY	1 cut(s)	598
XceI	RCATGY	1 cut(s)	1966
XcmI	CCANNNNNNNNNTGG	3 cut(s)	113, 575, 1091
XmaJI	CCTAGG	1 cut(s)	1334
XspI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2214
ZrmI	AGTACT	1 cut(s)	1759
Zsp2I	ATGCAT	1 cut(s)	1124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.