pycom10g24770

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007493
Physical Location & Seq
Reverse (-)
26536956 .. 26540590
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1500 bp
ATGGAGGCCTCTGGTTCGAACGGCGCGTCGAGTCTCGCTGACGATATGGCGCTGAGGGGTTTGCTGGAGGTGTTTGGGGCTGTGTTTTCTCTCGATCAAATCACTTCTGCGTATTGCAAGGCGGGGAAGAATGCTGACGACGCCGCCGAAGCTCTGGCTACCTCTGCACCCAATGGCGGAGAAACTTCCGGATTACCTGTCAATAGTATCTATAAGAAGCCGCCGTATCAAGAAAATGGGAATGGGAATAGGAACGGGAATGGGAATCCCAGAGCTTCAAAGCCGAAATATCGGCCGGTTTCGGTTGGTAGCGTTTCGAGCATTATTGGGAAGCAGTATGTGAAGGAAACGGCTCCGGCGCCGGCAAATGGGTCTAATGAAGCAACCAAGCCCTTGAAGCTGGACTCCAGTGTGTTGCCGATGTCGGGAATTTGGGTCGAAAACGATGAGTCTAGTTCCTCAAGGGATGAACGGCTCCACCAGGGCATCGAGGATTTTCTGTTCAGTATGCTTGGAGTTGGGTTCAAGCTAGAGAGGGATCAGATTCGGCAAGTTCTTGATAGTTGTAGATATGATATGGAAAAGAGCATGGAAAAACTGATTAATCTGCCAGCATCAACTTCCGAGAAAAGGAACGAACTCGTCAGAAAATCCAGTGAGAAGTCTGCAGATTTGTCACTGAAATATGAAGTATCATCCGACAGAAATTCCAAAAACTCCACCGAAGTTAACAGAGATAGTGCTTCAAATACAAACGGAGCAGAATTTACTGGACAGCAAAAGGAGAGAAATGATCTCGAGAAGGATATTCTGGCTTCTCTGTTTTGTGCTTCCGAGAGAACCGAGGAGAGACCAGTGGAGAGACCAAGAAGAACAATAAAGTCAGGGAGTGGATATGGAGCATATGGACAACTAGTTGCTGAACCTCCGAACGATTTCATTTCAGAGTACAAGTCAGCTGTTGTGTATCAACATCATCATGGCGAGGATGATGCAGATGACGAAGATAGCTACCAGGTTCTCCGTAGATCTGTGAAGGAGTATCGGAGTACAATGAAGGACTATTACCAGGCTGCTGTAGCCGCGTTTGCTAAGGAGGATCATGATCGAGCTTACAAACTTCTAGAACAAGGACATTTTTTCCTCAAAAAGGCGCGTGAAGCAGAGGATGAGTCGAATGAAGTGATTCTGAAAACTAGAAATGTTGAGACTCAAGGTGAAATAGTGCTTGACTTGCAAGAACGCAGTGCCAAGGAGGCAATACGTCTTTTGAAGTGTCAAATTTCCTCATTTTCTGGTATCTCATCAATCAAGTATCTGAAGGTCATATGCGATACAAAAGAGGAAGATATCTCAAAAGGATCTCGAAGACGATCATTGGTTCTGAAGCTACTGGAGGAAGAATCGATTAAGTGGACCGAAGGAGAAAATGCTGGAACAATACTAATCCAATTAGACAGTGTTAATCGAAAGCAATTAACATTTCTCACAAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771

Protein Analysis

500

Amino Acids

55.38

Weight (kDa)

5.62

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 338 - 401 1.4e-11 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 358
AccII CGCG 3 cut(s) 26, 1085, 1156
AccIII TCCGGA 1 cut(s) 188
AciI CCGC 5 cut(s) 122, 144, 177, 221, 1083
AclWI GGATC 3 cut(s) 546, 1107, 1369
AcoI YGGCCR 1 cut(s) 293
AcsI RAATTY 4 cut(s) 429, 706, 764, 1281
AcuI CTGAAG 2 cut(s) 1340, 1406
AcyI GRCGYC 2 cut(s) 141, 359
AfaI GTAC 2 cut(s) 950, 1051
AfiI CCNNNNNNNGG 5 cut(s) 176, 368, 425, 630, 1150
AgsI TTSAA 5 cut(s) 279, 397, 526, 747, 1273
AhlI ACTAGT 1 cut(s) 913
AjnI CCWGG 3 cut(s) 480, 1014, 1068
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
AluBI AGCT 8 cut(s) 152, 275, 400, 529, 959, 1011, 1112, 1390
AluI AGCT 8 cut(s) 152, 275, 400, 529, 959, 1011, 1112, 1390
Alw26I GTCTC 4 cut(s) 38, 844, 856, 1202
AlwI GGATC 3 cut(s) 546, 1107, 1369
Ama87I CYCGRG 1 cut(s) 797
Aor13HI TCCGGA 1 cut(s) 188
AoxI GGCC 2 cut(s) 6, 293
ApeKI GCWGC 1 cut(s) 1073
ApoI RAATTY 4 cut(s) 429, 706, 764, 1281
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AseI ATTAAT 1 cut(s) 603
Asp700I GAANNNNTTC 2 cut(s) 935, 1185
AspLEI GCGC 4 cut(s) 26, 52, 361, 1156
AspS9I GGNCC 1 cut(s) 1416
AsuHPI GGTGA 1 cut(s) 1229
AsuII TTCGAA 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 1 cut(s) 1416
BanI GGYRCC 1 cut(s) 358
BarI GAAGNNNNNNTAC 2 cut(s) 1049, 1081
BbsI GAAGAC 1 cut(s) 1375
BbvCI CCTCAGC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 1060
BceAI ACGGC 4 cut(s) 37, 208, 366, 488
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 3 cut(s) 482, 1016, 1070
BcoDI GTCTC 4 cut(s) 38, 844, 856, 1202
BcuI ACTAGT 1 cut(s) 913
BfaI CTAG 5 cut(s) 453, 530, 914, 1124, 1197
BfmI CTRYAG 2 cut(s) 666, 1077
BfoI RGCGCY 2 cut(s) 53, 362
BglI GCCNNNNNGGC 1 cut(s) 1256
BglII AGATCT 1 cut(s) 1028
BisI GCNGC 4 cut(s) 144, 221, 1074, 1083
BlsI GCNGC 4 cut(s) 145, 222, 1075, 1084
Bme1390I CCNGG 3 cut(s) 482, 1016, 1070
Bme18I GGWCC 1 cut(s) 1416
BmeT110I CYCGRG 1 cut(s) 797
BmgT120I GGNCC 1 cut(s) 1416
BmiI GGNNCC 3 cut(s) 354, 360, 476
BmrFI CCNGG 3 cut(s) 482, 1016, 1070
BmsI GCATC 3 cut(s) 495, 623, 982
BpiI GAAGAC 1 cut(s) 1375
BpmI CTGGAG 3 cut(s) 86, 391, 1415
Bpu10I CCTNAGC 2 cut(s) 53, 1092
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 2 cut(s) 445, 1197
Bsa29I ATCGAT 1 cut(s) 1406
BsaBI GATNNNNATC 1 cut(s) 1104
BsaHI GRCGYC 2 cut(s) 141, 359
BsaI GGTCTC 2 cut(s) 844, 856
BsaJI CCNNGG 3 cut(s) 481, 843, 1251
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 368, 425, 630, 1150
Bse118I RCCGGY 2 cut(s) 295, 361
Bse1I ACTGG 5 cut(s) 408, 654, 775, 854, 1398
Bse8I GATNNNNATC 1 cut(s) 1104
BseAI TCCGGA 1 cut(s) 188
BseBI CCWGG 3 cut(s) 482, 1016, 1070
BseCI ATCGAT 1 cut(s) 1406
BseDI CCNNGG 3 cut(s) 481, 843, 1251
BseGI GGATG 4 cut(s) 472, 695, 994, 1174
BseJI GATNNNNATC 1 cut(s) 1104
BseLI CCNNNNNNNGG 5 cut(s) 176, 368, 425, 630, 1150
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 5 cut(s) 408, 654, 775, 854, 1398
BseRI GAGGAG 1 cut(s) 860
BseX3I CGGCCG 1 cut(s) 293
BseXI GCAGC 1 cut(s) 1060
BsgI GTGCAG 1 cut(s) 150
Bsh1236I CGCG 3 cut(s) 26, 1085, 1156
Bsh1285I CGRYCG 1 cut(s) 296
BshFI GGCC 2 cut(s) 8, 295
BshNI GGYRCC 1 cut(s) 358
BshVI ATCGAT 1 cut(s) 1406
BsiEI CGRYCG 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 797
BsiSI CCGG 4 cut(s) 189, 296, 356, 362
BslI CCNNNNNNNGG 5 cut(s) 176, 368, 425, 630, 1150
BsmAI GTCTC 4 cut(s) 38, 844, 856, 1202
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 2 cut(s) 8, 295
Bso31I GGTCTC 2 cut(s) 844, 856
BsoBI CYCGRG 1 cut(s) 797
Bsp119I TTCGAA 1 cut(s) 17
Bsp13I TCCGGA 1 cut(s) 188
Bsp143I GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
BspACI CCGC 5 cut(s) 122, 144, 177, 221, 1083
BspANI GGCC 2 cut(s) 8, 295
BspCNI CTCAG 1 cut(s) 45
BspDI ATCGAT 1 cut(s) 1406
BspEI TCCGGA 1 cut(s) 188
BspFNI CGCG 3 cut(s) 26, 1085, 1156
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 3 cut(s) 354, 360, 476
BspMAI CTGCAG 1 cut(s) 670
BspPI GGATC 3 cut(s) 546, 1107, 1369
BspT104I TTCGAA 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 358
BspTNI GGTCTC 2 cut(s) 844, 856
BsrFI RCCGGY 2 cut(s) 295, 361
BsrI ACTGG 5 cut(s) 408, 654, 775, 854, 1398
BssAI RCCGGY 2 cut(s) 295, 361
BssECI CCNNGG 3 cut(s) 481, 843, 1251
BssMI GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
BssNI GRCGYC 2 cut(s) 141, 359
BssT1I CCWWGG 1 cut(s) 1251
Bst2UI CCWGG 3 cut(s) 482, 1016, 1070
Bst4CI ACNGT 1 cut(s) 1460
BstACI GRCGYC 2 cut(s) 141, 359
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 363, 612
BstDEI CTNAG 2 cut(s) 53, 1092
BstENI CCTNNNNNAGG 1 cut(s) 1148
BstF5I GGATG 4 cut(s) 472, 695, 994, 1174
BstFNI CGCG 3 cut(s) 26, 1085, 1156
BstH2I RGCGCY 2 cut(s) 53, 362
BstHHI GCGC 4 cut(s) 26, 52, 361, 1156
BstKTI GATC 8 cut(s) 97, 541, 796, 1031, 1102, 1108, 1364, 1376
BstMAI GTCTC 4 cut(s) 38, 844, 856, 1202
BstMBI GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
BstMCI CGRYCG 1 cut(s) 296
BstNI CCWGG 3 cut(s) 482, 1016, 1070
BstSCI CCNGG 3 cut(s) 480, 1014, 1068
BstSFI CTRYAG 2 cut(s) 666, 1077
BstUI CGCG 3 cut(s) 26, 1085, 1156
BstV1I GCAGC 1 cut(s) 1060
BstV2I GAAGAC 1 cut(s) 1375
BstX2I RGATCY 2 cut(s) 1028, 1361
BstYI RGATCY 2 cut(s) 1028, 1361
BstZI CGGCCG 1 cut(s) 293
Bsu15I ATCGAT 1 cut(s) 1406
BsuRI GGCC 2 cut(s) 8, 295
BsuTUI ATCGAT 1 cut(s) 1406
BtsCI GGATG 4 cut(s) 472, 695, 994, 1174
BtsI GCAGTG 1 cut(s) 1252
BtsIMutI CAGTG 6 cut(s) 415, 661, 677, 861, 1252, 1465
Cac8I GCNNGC 2 cut(s) 363, 612
CciI TCATGA 1 cut(s) 1102
CfoI GCGC 4 cut(s) 26, 52, 361, 1156
Cfr10I RCCGGY 2 cut(s) 295, 361
Cfr13I GGNCC 1 cut(s) 1416
ClaI ATCGAT 1 cut(s) 1406
CseI GACGC 2 cut(s) 15, 149
CsiI ACCWGGT 1 cut(s) 1014
Csp6I GTAC 2 cut(s) 949, 1050
CviAII CATG 3 cut(s) 589, 980, 1103
CviQI GTAC 2 cut(s) 949, 1050
DdeI CTNAG 2 cut(s) 53, 1092
DinI GGCGCC 1 cut(s) 360
DpnI GATC 8 cut(s) 96, 540, 795, 1030, 1101, 1107, 1363, 1375
DpnII GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
EaeI YGGCCR 1 cut(s) 293
EagI CGGCCG 1 cut(s) 293
EciI GGCGGA 1 cut(s) 192
EclXI CGGCCG 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 1251
Eco147I AGGCCT 1 cut(s) 8
Eco31I GGTCTC 2 cut(s) 844, 856
Eco32I GATATC 1 cut(s) 1351
Eco47I GGWCC 1 cut(s) 1416
Eco52I CGGCCG 1 cut(s) 293
Eco57I CTGAAG 2 cut(s) 1340, 1406
Eco88I CYCGRG 1 cut(s) 797
EcoNI CCTNNNNNAGG 1 cut(s) 1148
EcoRII CCWGG 3 cut(s) 480, 1014, 1068
EcoRV GATATC 1 cut(s) 1351
EcoT14I CCWWGG 1 cut(s) 1251
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 1251
FaeI CATG 3 cut(s) 592, 983, 1106
FatI CATG 3 cut(s) 588, 979, 1102
FauI CCCGC 1 cut(s) 115
FauNDI CATATG 2 cut(s) 904, 1328
Fnu4HI GCNGC 4 cut(s) 144, 221, 1074, 1083
FokI GGATG 4 cut(s) 479, 682, 1001, 1181
Fsp4HI GCNGC 4 cut(s) 144, 221, 1074, 1083
FspBI CTAG 5 cut(s) 453, 530, 914, 1124, 1197
GlaI GCGC 4 cut(s) 25, 51, 360, 1155
GluI GCNGC 4 cut(s) 144, 221, 1074, 1083
GsuI CTGGAG 3 cut(s) 86, 391, 1415
HaeII RGCGCY 2 cut(s) 53, 362
HaeIII GGCC 2 cut(s) 8, 295
HapII CCGG 4 cut(s) 189, 296, 356, 362
HgaI GACGC 2 cut(s) 15, 149
HhaI GCGC 4 cut(s) 26, 52, 361, 1156
Hin1I GRCGYC 2 cut(s) 141, 359
Hin1II CATG 3 cut(s) 592, 983, 1106
Hin6I GCGC 4 cut(s) 24, 50, 359, 1154
HinP1I GCGC 4 cut(s) 24, 50, 359, 1154
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HinfI GANTC 9 cut(s) 31, 265, 404, 449, 544, 1172, 1186, 1210, 1403
HpaI GTTAAC 1 cut(s) 730
HpaII CCGG 4 cut(s) 189, 296, 356, 362
HphI GGTGA 1 cut(s) 1229
Hpy166II GTNNAC 2 cut(s) 730, 1416
Hpy8I GTNNAC 2 cut(s) 730, 1416
Hpy99I CGWCG 2 cut(s) 31, 143
HpyAV CCTTC 6 cut(s) 337, 796, 1030, 1051, 1315, 1415
HpyCH4III ACNGT 1 cut(s) 1460
HpyCH4IV ACGT 1 cut(s) 1264
HpyCH4V TGCA 5 cut(s) 117, 167, 668, 995, 1237
HpyF3I CTNAG 2 cut(s) 53, 1092
HpySE526I ACGT 1 cut(s) 1264
Hsp92I GRCGYC 2 cut(s) 141, 359
Hsp92II CATG 3 cut(s) 592, 983, 1106
HspAI GCGC 4 cut(s) 24, 50, 359, 1154
KasI GGCGCC 1 cut(s) 358
Kpn2I TCCGGA 1 cut(s) 188
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
KspAI GTTAAC 1 cut(s) 730
Kzo9I GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
LmnI GCTCC 4 cut(s) 358, 480, 758, 899
Lsp1109I GCAGC 1 cut(s) 1060
LweI GCATC 3 cut(s) 495, 623, 982
MabI ACCWGGT 1 cut(s) 1014
MaeI CTAG 5 cut(s) 453, 530, 914, 1124, 1197
MaeII ACGT 1 cut(s) 1264
MaeIII GTNAC 1 cut(s) 675
MalI GATC 8 cut(s) 96, 540, 795, 1030, 1101, 1107, 1363, 1375
MboI GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
MboII GAAGA 6 cut(s) 139, 882, 1016, 1358, 1380, 1412
MflI RGATCY 2 cut(s) 1028, 1361
MluCI AATT 6 cut(s) 429, 706, 764, 1281, 1451, 1475
Mly113I GGCGCC 1 cut(s) 359
MlyI GAGTC 5 cut(s) 40, 398, 458, 1181, 1204
MmeI TCCRAC 1 cut(s) 723
MroI TCCGGA 1 cut(s) 188
MroNI GCCGGC 1 cut(s) 361
MroXI GAANNNNTTC 2 cut(s) 935, 1185
MseI TTAA 5 cut(s) 603, 729, 1410, 1464, 1478
MslI CAYNNNNRTG 1 cut(s) 978
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 4 cut(s) 189, 296, 356, 362
MspR9I CCNGG 3 cut(s) 482, 1016, 1070
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 3 cut(s) 482, 1016, 1070
MvnI CGCG 3 cut(s) 26, 1085, 1156
NaeI GCCGGC 1 cut(s) 363
NarI GGCGCC 1 cut(s) 359
NdeI CATATG 2 cut(s) 904, 1328
NdeII GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
NgoMIV GCCGGC 1 cut(s) 361
NlaIII CATG 3 cut(s) 592, 983, 1106
NlaIV GGNNCC 3 cut(s) 354, 360, 476
NmuCI GTSAC 1 cut(s) 675
NspV TTCGAA 1 cut(s) 17
PaeR7I CTCGAG 1 cut(s) 797
PagI TCATGA 1 cut(s) 1102
PceI AGGCCT 1 cut(s) 8
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 144
PctI GAATGC 1 cut(s) 136
PdiI GCCGGC 1 cut(s) 363
PdmI GAANNNNTTC 2 cut(s) 935, 1185
PfeI GAWTC 4 cut(s) 265, 544, 1186, 1403
PkrI GCNGC 4 cut(s) 145, 222, 1075, 1084
PleI GAGTC 5 cut(s) 39, 398, 457, 1180, 1204
PluTI GGCGCC 1 cut(s) 362
PpsI GAGTC 5 cut(s) 39, 398, 457, 1180, 1204
PshBI ATTAAT 1 cut(s) 603
Psp6I CCWGG 3 cut(s) 480, 1014, 1068
PspGI CCWGG 3 cut(s) 480, 1014, 1068
PspN4I GGNNCC 3 cut(s) 354, 360, 476
PspPI GGNCC 1 cut(s) 1416
PstI CTGCAG 1 cut(s) 670
PsuI RGATCY 2 cut(s) 1028, 1361
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 2 cut(s) 950, 1051
RsaNI GTAC 2 cut(s) 949, 1050
RseI CAYNNNNRTG 1 cut(s) 978
SaqAI TTAA 5 cut(s) 603, 729, 1410, 1464, 1478
SatI GCNGC 4 cut(s) 144, 221, 1074, 1083
Sau3AI GATC 8 cut(s) 94, 538, 793, 1028, 1099, 1105, 1361, 1373
Sau96I GGNCC 1 cut(s) 1416
SchI GAGTC 5 cut(s) 40, 398, 458, 1181, 1204
ScrFI CCNGG 3 cut(s) 482, 1016, 1070
SexAI ACCWGGT 1 cut(s) 1014
SfaNI GCATC 3 cut(s) 495, 623, 982
SfcI CTRYAG 2 cut(s) 666, 1077
SfoI GGCGCC 1 cut(s) 360
Sfr274I CTCGAG 1 cut(s) 797
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 1 cut(s) 1416
SlaI CTCGAG 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 978
SmlI CTYRAG 3 cut(s) 460, 797, 1212
SmoI CTYRAG 3 cut(s) 460, 797, 1212
SpeI ACTAGT 1 cut(s) 913
Sse9I AATT 6 cut(s) 429, 706, 764, 1281, 1451, 1475
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 5 cut(s) 122, 144, 177, 221, 1083
SspDI GGCGCC 1 cut(s) 358
SspMI CTAG 5 cut(s) 453, 530, 914, 1124, 1197
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 480, 1014, 1068
StyI CCWWGG 1 cut(s) 1251
TaaI ACNGT 1 cut(s) 1460
TaiI ACGT 1 cut(s) 1267
TaqII GACCGA 1 cut(s) 1433
TasI AATT 6 cut(s) 429, 706, 764, 1281, 1451, 1475
TatI WGTACW 2 cut(s) 948, 1049
TauI GCSGC 3 cut(s) 146, 223, 1085
TfiI GAWTC 4 cut(s) 265, 544, 1186, 1403
Tru1I TTAA 5 cut(s) 603, 729, 1410, 1464, 1478
Tru9I TTAA 5 cut(s) 603, 729, 1410, 1464, 1478
TscAI CASTG 6 cut(s) 415, 661, 684, 861, 1252, 1465
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 1 cut(s) 1073
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 6 cut(s) 393, 483, 702, 928, 1070, 1194
TspGWI ACGGA 2 cut(s) 771, 1013
TspRI CASTG 6 cut(s) 415, 661, 684, 861, 1252, 1465
VpaK11BI GGWCC 1 cut(s) 1416
VspI ATTAAT 1 cut(s) 603
XagI CCTNNNNNAGG 1 cut(s) 1148
XapI RAATTY 4 cut(s) 429, 706, 764, 1281
XbaI TCTAGA 1 cut(s) 1123
XhoI CTCGAG 1 cut(s) 797
XmnI GAANNNNTTC 2 cut(s) 935, 1185
XspI CTAG 5 cut(s) 453, 530, 914, 1124, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.