pycom10g27020

Abnormal spindle-like microcephaly-associated protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006240
Physical Location & Seq
Forward (+)
28128839 .. 28136004
7166 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4461 bp
ATGGAAGGCCGAGAGCCACCGCCTTCTCCGTCGCCGTCGCCTTACAGAAACCCCTCCACCCTCTTCAAAGACATCACCAATTTCAAAACCCCTAAAGCCTCCTCGCGAATCTCCAATTTCCAGTCCCCCGGCCCCAAATTCTTCACTGCCTCGAAACAAACCCCTGGTACTTCTTCGTCATTTCGCCGCCGGCCGTCTCTCGCTCTATCAAACTCTGGCCGGATAAAGGCGGCGTCCCGGAAACTCAAGGCCTTTGAGCTCGAGCAGTCGCAATCGTCCCGCAAGGCCCAAATTCAGAAAGAGCAATCGCTCAAGTCGCTGGCCAAGTCTCTCTCCGTCTGGCTCAATTTCCTCTTCCAAAACCCTACATCCTGCGGCTGCAATTTGTCCGTCGATGGAGACCGCACGGGAACGCTCCCTAAGGGAAAGAGGGATAGCGATCCCGGCAGTGCGGTTGGGGTCGATTCGGCGTGGCGGGACCCCAAGCGTCAGAGAGACTCTTCGTGGCGGGCCGTGAGCGCTGTAGCGTTTTCGAGCTCCAAGTATTTGAACCTGCGTTCTTCGTTGGAGCATGTGTGCAGTGTTGATGATTTGACGCAGAGGATGCGACTCTATCTGAGCATGGGCAGTTGCAAGGAGGTTTTCGATGCGATGACTCATGTAGCAAAGAATATTGATGAAGGGAGATTAAAGATGAAGGCACATTGCCCTTTAGTTACAGATTTTGGGTTGAAGGAGAAGGCCACTAGAATCCTCATGAGTTATAACCCAATTTGGCTTCGAATTGGATTGTACATTGTTTTCGGTGGTGATTCTTTGTTGTCTGACACAGATGCTAATTCTGATGAAGAACTCAGGTTTCTGAAAATGATCATTGAGAAGCAATTTTTTGCACATTCGGGTCTAGCGAAAGATTATGCTTACAACAAGAAGGTTGAGGGTCTTTACAGACCGGGTTATTACGAAGCTCTGGGAAATGTCACTTTGAAGAGATTTCTGATGCTCACTCTTATCCTTGATAGAGCGAAATGCCAGAGCAGTCTTTCTCTTAAGTATGGTATTGATGGAGTGGATGGGGGTTCTCCTTTATTGTTTACAGTTGAATCTCATATTAAATCAAGTCATCAGGTGATCCATGATTTTCTGTCATCTGATGTCATGCTTGGCGAAGGTAATATTTCAGCACATCTGGTGGTTTTAGGATACAAAGTGTCCTATCAGCAGCATCCACTTGTTGAATATGACTTTCGAGTTACCGATTTATTTGCTGATCTTCAAGATGGGGTGCGCCTGTGCAGAGCCATTCAACTTTTGCTAGATGACGCCTCTATCCTCACGAAAATGGTTGTTCCAGCAGATACACTTAAGAAGAACATGGCAAATTGTGGCATTGCCCTGCAATACCTTAAACAGGCTGGCGTTGTTTTACATGATGAAGATGGAATGATGATTGTGGCAAATGATATTGCTGGCGGTGACAAGGAACTTACTCTTTCCTTGCTCTGGAACATGTTTGTCCATTTGCAGCTACCGCTTCTGGTCAAGAAAACAAACTTAGCTGAGGAAATTTGCAAAATTCGTGGCAGTGCAATTTGTGAAAATTATGATTGCAAGGTTGACAGTTTTGCTTCATTGGTTGATGGAAAAGCCATATGGTGCTTGCTGGACTATTACTTTCGTAAGCAACTATGTTGTGGTCGGTCATCAAAGGATCGTAATAAATCAAGTCACGAAGAATCAATCATGTTGGCCACTGATTATTCAGATGCAGTGCACAACTTTTTATTGTCCCAGAAATTGTTGACGTTATTGGGAAATTTTCCAGAGGTTCTGCAAATTAGTGACATTCTTGAATACAACGGTGCACGAAATGACCGGAGTGTGGTGATACTATTAGTGTTCCTTTCATCTCAACTTATTGTCAAGAAAAATATGGACCAATTAAATTTCCATAAACTCTTGGGTTGTGATTGCCAAAGTACAGACAGAAAATATACATGCATGGGATGCTATGTAAGACCCGAAGCAACACAGATCAAAGAGGAAACATATGACCACCACACCGAAGGTTTCTTTCCTTTGTTTTCTTGTATCTGTCTTCAAGATTCTGTAAGAAAGTTTAAGGCTATTCAGGCCTGGTGGCAAGATATGGCTGAAAGGAACCATAAATCTGTTGCAAAACCAACTTCTCCGACTTCGCTTAACCTATCTACGAATAAGGATAGCGTTAATATTGAAAAAGTAAATACTGACAAAGGCAATTATGAGGGAGGAAGTATTGTAACTCATGATATATCTGCTCCTGATCTAGTCAAAGCTGCCATTGTTGTCCAAAGATATATTCGTGGGTGGCTTGCCAGGTTGAGATACATTCATGGGGTTGCTCTTGTAGATAAATCTTGCAATCTGTGCCAAGAAAATGTTGCACGTGATCTTCAAGTAAGGGCAGAAAATACTGAAAAAAGAAATTATGAGGGAGGAAGTATTGTAACTCATGATATATCTGCTCCTGATCTAGTCAAAGCTGCCATTGTTGTCCAAAGATATATTCGTGGGTGGCTTGCAAGGTTGAGATACATTCATGGGGTTACTCTTGTGGATAAATCTTGCAATCTGTGCCAAGAAAATGTTGCACGTGATCTTCAAGTAAGGGCAGAGAATACTGAAAAAAGAAATTATGAGGGAGGAAGTATTGTAACTCCTGATATATCTGCTCCTGATCTAGTCAAAGCTGCCATTGTTGTCCAAAGATATATTCGTGGGTGGTTTGTAAGGTTGAGATACATTCATGGGGTTGCTCTTGTAGATAAATCTTGCAATCTGTGCCAAGAAAATGTTGCACGTGACCTTCAAGTAAGGGCAGAAAATACTGAAAAAAGCAATTATGAGGGAGGAAGTATTGTAACTCATGATTTATCTGCTCCTGATCGATCCATAGCTGCCATTGTTGTCCAAAGATATATTCGTGGGTGGCTTGCAAGGTTGAGATACATTCATGGGGTTACTCTTGTAGATAAATCTTGCAATCTGTGCCAAGAAAGTGTTGCGCGTGATCTTCAAGTAAGGGCAGCAATTAAAATCCAGTTTGCATGGAAGAATTTTTCTGTCCGTCATTCTCTTCTCTATCAGCATTCCGCTGCAACTAAAATTCAAAGTCATTTTCGTGGTTGGCTTTTGAGGAGGAGGTTTCATATTAACAGGCAAGCAATAATAAAAATCCAAAGTGTCTATCGAATGTCAAAATGCTGGAAGGCTTATCAGCAATACAAAATTGCAGCTGTTTCAGCCACCGTCATTCAATCTTATATACGTGGGTGTTTTGCCCGGAAAGGCGCTGAAAATCATAGGCTTCTCATTGTTGCAATCCAAAGATACTTCCGTGGTTGGCTGATCAGAAGTCAATTCTCGTGTCAAAGACAGGCTGCAGTAAAGATCCAAAGCGCTATTCGAGGCTTGATATGGTGTCAATCATTTCATCGTCATAGACAAGCTGCTGTGGATATTCAACGAATAGTCAGGGGGCAGATTAGTCGAAATAGGCTCTTAGGTGCTGCTTCACTACGCCCAATCGTCCGCAATGGTTGTCCTTTGAATAGCACAGGAGCCCTTTATATGGGTGCTGAACTAAATAAAGTTGTTTGCTCTGTGTTGAAATTGCAAAGGTGGTGGAGGAGTGTTATGTCACTAAACTTCAGAACGAATTCTGCGGTCATTATTCAGTCTCATATTCGAGGGTGGTTTGCTAGGCAAAAAGCTGCTAGAGAAAGGCAATGTATTGTTGTGATACAATCATGCTGGAGAGGCTACCTTCTAAGGAGGAAAGAGACGGAAGGTCAGCTACTGGAGCTGCGCTTGAGAGTGCAAAAGTCTGCCACGAATATAGATGATAGCATGCGGATTATAAACAGACTTGTAGCAGCACTTTCAGAGCTACAAACAATGAAAAGTATCAGCAGCATTCTTCACACTTGTGTTACTTTGGACAAGGCCACTGCACATTCTCAGAAATGTTGTGAAAAACTCGTGGAGGAAGGAGCTGTTAAAACATTGCTGAAGATTTTTGGCTCAGCCACCCGAAGTATACCCGATCAGGAGGTTCAAAAACACGTGCTCTCAACTCTAAGGAACCTTGCCCGCTATCCACACCTGGTAGAAGTGCTAATTGACAGTCCGGGATCAGTAGAAACTGTTGTATGCGAGTTTGTAAGGAACAAGGAAGAGGGTTATTTCACCGCTTCCGAACTTCTGAAGAAGGTTTGTTCTAGCAGTAAAGGCATTGAAGCTGTGCGTAGATCGCCCGCTCTTTTGAAAAGGCTACACAATCTTGTTGAGGACCTTAGTAGGAAGGCTGGCAACAATGACAAGAGGAATGCCGGGCGAGTTACAGTTGCTAGAGAAGCTACAGAAAGGAGATTGAGTGAGGCTGTTGAAATTATGAAGATGGTAAAACAGAGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm CH IQ

Protein Analysis

1487

Amino Acids

167.88

Weight (kDa)

9.54

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 1044 - 1064 1.2e-06 IQ calmodulin-binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 765, 3902
Acc36I ACCTGC 1 cut(s) 561
AccBSI CCGCTC 1 cut(s) 4301
AccI GTMKAC 1 cut(s) 4081
AccII CGCG 2 cut(s) 106, 3049
AclWI GGATC 6 cut(s) 434, 1126, 1719, 2925, 3427, 4183
AcoI YGGCCR 4 cut(s) 191, 217, 321, 1749
AcsI RAATTY 9 cut(s) 137, 291, 1566, 1575, 1816, 1945, 3097, 3147, 3700
AcuI CTGAAG 3 cut(s) 3676, 4073, 4268
AcvI CACGTG 4 cut(s) 2429, 2636, 2843, 4108
AcyI GRCGYC 2 cut(s) 233, 1323
AfaI GTAC 4 cut(s) 169, 794, 1981, 4457
AfeI AGCGCT 2 cut(s) 520, 3442
AfiI CCNNNNNNNGG 5 cut(s) 226, 1411, 1503, 1882, 3613
AflII CTTAAG 2 cut(s) 1049, 1364
AflIII ACRYGT 2 cut(s) 1509, 4105
AhdI GACNNNNNGTC 1 cut(s) 3831
AjnI CCWGG 4 cut(s) 163, 2135, 2357, 4146
AleI CACNNNNGTG 1 cut(s) 3969
Alw21I GWGCWC 5 cut(s) 261, 539, 1776, 1867, 4113
Alw26I GTCTC 6 cut(s) 201, 333, 393, 489, 3726, 3818
Alw44I GTGCAC 2 cut(s) 1772, 1863
AlwI GGATC 6 cut(s) 434, 1126, 1719, 2925, 3427, 4183
AlwNI CAGNNNCTG 1 cut(s) 3841
Ama87I CYCGRG 1 cut(s) 260
Aor51HI AGCGCT 2 cut(s) 520, 3442
ApaLI GTGCAC 2 cut(s) 1772, 1863
ApoI RAATTY 9 cut(s) 137, 291, 1566, 1575, 1816, 1945, 3097, 3147, 3700
Asp700I GAANNNNTTC 1 cut(s) 3700
AspLEI GCGC 6 cut(s) 521, 1290, 3049, 3335, 3443, 3852
AspS9I GGNCC 6 cut(s) 131, 286, 478, 510, 1936, 4333
AsuC2I CCSGG 7 cut(s) 129, 238, 444, 954, 3325, 4173, 4375
AsuHPI GGTGA 6 cut(s) 67, 821, 1141, 1487, 1897, 4222
AsuII TTCGAA 1 cut(s) 781
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 3 cut(s) 478, 1936, 4333
AxyI CCTNAGG 1 cut(s) 420
BaeGI GKGCMC 2 cut(s) 1776, 1867
BaeI ACNNNNGTAYC 2 cut(s) 3364, 3397
BalI TGGCCA 2 cut(s) 323, 1751
BanII GRGCYC 3 cut(s) 261, 539, 3607
BarI GAAGNNNNNNTAC 2 cut(s) 3822, 3854
BauI CACGAG 2 cut(s) 3406, 4022
BbrPI CACGTG 4 cut(s) 2429, 2636, 2843, 4108
BbsI GAAGAC 1 cut(s) 2090
Bbv12I GWGCWC 5 cut(s) 261, 539, 1776, 1867, 4113
BbvCI CCTCAGC 1 cut(s) 1560
BccI CCATC 7 cut(s) 389, 1058, 1067, 1274, 1433, 1634, 4435
BceAI ACGGC 3 cut(s) 19, 178, 497
BcgI CGANNNNNNTGC 4 cut(s) 1247, 1281, 3512, 3546
BciT130I CCWGG 4 cut(s) 165, 2137, 2359, 4148
BciVI GTATCC 1 cut(s) 1196
BclI TGATCA 2 cut(s) 870, 3390
BcnI CCSGG 7 cut(s) 129, 238, 444, 954, 3325, 4173, 4375
BcoDI GTCTC 6 cut(s) 201, 333, 393, 489, 3726, 3818
BfmI CTRYAG 3 cut(s) 522, 3423, 4401
BfoI RGCGCY 3 cut(s) 522, 3336, 3444
BfrI CTTAAG 2 cut(s) 1049, 1364
BfuAI ACCTGC 1 cut(s) 561
BfuI GTATCC 1 cut(s) 1196
BlpI GCTNAGC 1 cut(s) 4066
Bme18I GGWCC 3 cut(s) 478, 1936, 4333
BmeRI GACNNNNNGTC 1 cut(s) 3831
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 6 cut(s) 131, 286, 478, 510, 1936, 4333
BmiI GGNNCC 6 cut(s) 133, 479, 480, 2162, 3604, 4127
BmsI GCATC 7 cut(s) 594, 637, 823, 990, 1234, 1756, 1997
BpiI GAAGAC 1 cut(s) 2090
BpmI CTGGAG 2 cut(s) 3817, 3863
Bpu10I CCTNAGC 1 cut(s) 1560
Bpu1102I GCTNAGC 1 cut(s) 4066
Bpu14I TTCGAA 1 cut(s) 781
BpuEI CTTGAG 3 cut(s) 230, 296, 3874
BpuMI CCSGG 7 cut(s) 129, 238, 444, 954, 3325, 4173, 4375
Bsa29I ATCGAT 1 cut(s) 2929
BsaAI YACGTR 5 cut(s) 2429, 2636, 2843, 3311, 4108
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 233, 1323
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 127, 163, 3379
BsaWI WCCGGW 1 cut(s) 1875
BsaXI ACNNNNNCTCC 4 cut(s) 560, 590, 4402, 4432
Bsc4I CCNNNNNNNGG 5 cut(s) 226, 1411, 1503, 1882, 3613
Bse118I RCCGGY 1 cut(s) 189
Bse1I ACTGG 3 cut(s) 121, 3082, 3846
Bse21I CCTNAGG 1 cut(s) 420
Bse3DI GCAATG 5 cut(s) 703, 1389, 3583, 3776, 4046
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 4 cut(s) 165, 2137, 2359, 4148
BseCI ATCGAT 1 cut(s) 2929
BseDI CCNNGG 3 cut(s) 127, 163, 3379
BseGI GGATG 5 cut(s) 368, 609, 1078, 1225, 2012
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 5 cut(s) 226, 1411, 1503, 1882, 3613
BseMI GCAATG 5 cut(s) 703, 1389, 3583, 3776, 4046
BseMII CTCAG 5 cut(s) 608, 868, 1551, 4016, 4080
BseNI ACTGG 3 cut(s) 121, 3082, 3846
BseRI GAGGAG 4 cut(s) 91, 3193, 3196, 3685
BseSI GKGCMC 2 cut(s) 1776, 1867
BseX3I CGGCCG 1 cut(s) 191
BsgI GTGCAG 3 cut(s) 598, 1315, 3978
Bsh1236I CGCG 2 cut(s) 106, 3049
Bsh1285I CGRYCG 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 2929
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 5 cut(s) 261, 539, 1776, 1867, 4113
BsiHKCI CYCGRG 1 cut(s) 260
BslFI GGGAC 5 cut(s) 109, 220, 262, 491, 1774
BslI CCNNNNNNNGG 5 cut(s) 226, 1411, 1503, 1882, 3613
BsmAI GTCTC 6 cut(s) 201, 333, 393, 489, 3726, 3818
BsmBI CGTCTC 2 cut(s) 201, 3818
BsmFI GGGAC 5 cut(s) 109, 220, 262, 491, 1774
BsmI GAATGC 3 cut(s) 3130, 3957, 4375
Bso31I GGTCTC 1 cut(s) 393
BsoBI CYCGRG 1 cut(s) 260
Bsp119I TTCGAA 1 cut(s) 781
Bsp1286I GDGCHC 6 cut(s) 261, 539, 1776, 1867, 3607, 4113
Bsp1407I TGTACA 1 cut(s) 792
Bsp1720I GCTNAGC 1 cut(s) 4066
Bsp68I TCGCGA 1 cut(s) 106
BspCNI CTCAG 5 cut(s) 609, 867, 1552, 4015, 4079
BspDI ATCGAT 1 cut(s) 2929
BspFNI CGCG 2 cut(s) 106, 3049
BspHI TCATGA 4 cut(s) 756, 2287, 2494, 2908
BspLI GGNNCC 6 cut(s) 133, 479, 480, 2162, 3604, 4127
BspMAI CTGCAG 1 cut(s) 3427
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 6 cut(s) 434, 1126, 1719, 2925, 3427, 4183
BspT104I TTCGAA 1 cut(s) 781
BspTI CTTAAG 2 cut(s) 1049, 1364
BspTNI GGTCTC 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 4301
BsrDI GCAATG 5 cut(s) 703, 1389, 3583, 3776, 4046
BsrFI RCCGGY 1 cut(s) 189
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 3 cut(s) 121, 3082, 3846
BssAI RCCGGY 1 cut(s) 189
BssECI CCNNGG 3 cut(s) 127, 163, 3379
BssNAI GTATAC 1 cut(s) 4082
BssNI GRCGYC 2 cut(s) 233, 1323
BssSI CACGAG 2 cut(s) 3406, 4022
Bst1107I GTATAC 1 cut(s) 4082
Bst2BI CACGAG 2 cut(s) 3406, 4022
Bst2UI CCWGG 4 cut(s) 165, 2137, 2359, 4148
Bst4CI ACNGT 7 cut(s) 1099, 1622, 1862, 3292, 4169, 4189, 4387
Bst6I CTCTTC 6 cut(s) 68, 359, 505, 983, 3123, 4212
BstACI GRCGYC 2 cut(s) 233, 1323
BstAFI CTTAAG 2 cut(s) 1049, 1364
BstAPI GCANNNNNTGC 6 cut(s) 604, 2007, 2409, 2616, 2823, 3030
BstAUI TGTACA 1 cut(s) 792
BstBAI YACGTR 5 cut(s) 2429, 2636, 2843, 3311, 4108
BstBI TTCGAA 1 cut(s) 781
BstDSI CCRYGG 1 cut(s) 3379
BstENI CCTNNNNNAGG 1 cut(s) 1409
BstF5I GGATG 5 cut(s) 368, 609, 1078, 1225, 2012
BstFNI CGCG 2 cut(s) 106, 3049
BstH2I RGCGCY 3 cut(s) 522, 3336, 3444
BstHHI GCGC 6 cut(s) 521, 1290, 3049, 3335, 3443, 3852
BstMAI GTCTC 6 cut(s) 201, 333, 393, 489, 3726, 3818
BstMCI CGRYCG 1 cut(s) 194
BstNI CCWGG 4 cut(s) 165, 2137, 2359, 4148
BstNSI RCATGY 4 cut(s) 575, 1513, 2001, 3895
BstSFI CTRYAG 3 cut(s) 522, 3423, 4401
BstSLI GKGCMC 2 cut(s) 1776, 1867
BstUI CGCG 2 cut(s) 106, 3049
BstV2I GAAGAC 1 cut(s) 2090
BstX2I RGATCY 1 cut(s) 3432
BstYI RGATCY 1 cut(s) 3432
BstZ17I GTATAC 1 cut(s) 4082
BstZI CGGCCG 1 cut(s) 191
Bsu15I ATCGAT 1 cut(s) 2929
Bsu36I CCTNAGG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 1196
BsuTUI ATCGAT 1 cut(s) 2929
BtgI CCRYGG 1 cut(s) 3379
BtgZI GCGATG 1 cut(s) 665
BtsCI GGATG 5 cut(s) 368, 609, 1078, 1225, 2012
BtsI GCAGTG 6 cut(s) 144, 454, 586, 1591, 1776, 3990
BtsIMutI CAGTG 7 cut(s) 144, 454, 586, 1591, 1752, 1776, 3990
BtuMI TCGCGA 1 cut(s) 106
BveI ACCTGC 1 cut(s) 561
CaiI CAGNNNCTG 1 cut(s) 3841
CciI TCATGA 4 cut(s) 756, 2287, 2494, 2908
CfoI GCGC 6 cut(s) 521, 1290, 3049, 3335, 3443, 3852
Cfr10I RCCGGY 1 cut(s) 189
Cfr13I GGNCC 6 cut(s) 131, 286, 478, 510, 1936, 4333
ClaI ATCGAT 1 cut(s) 2929
CseI GACGC 4 cut(s) 222, 476, 604, 1331
CsiI ACCWGGT 1 cut(s) 4146
Csp6I GTAC 4 cut(s) 168, 793, 1980, 4456
CspCI CAANNNNNGTGG 4 cut(s) 1561, 1596, 3647, 3682
CviQI GTAC 4 cut(s) 168, 793, 1980, 4456
DriI GACNNNNNGTC 1 cut(s) 3831
EaeI YGGCCR 4 cut(s) 191, 217, 321, 1749
EagI CGGCCG 1 cut(s) 191
Eam1104I CTCTTC 6 cut(s) 68, 359, 505, 983, 3123, 4212
Eam1105I GACNNNNNGTC 1 cut(s) 3831
EarI CTCTTC 6 cut(s) 68, 359, 505, 983, 3123, 4212
Ecl136II GAGCTC 2 cut(s) 259, 537
EclXI CGGCCG 1 cut(s) 191
Eco147I AGGCCT 2 cut(s) 251, 2135
Eco24I GRGCYC 3 cut(s) 261, 539, 3607
Eco31I GGTCTC 1 cut(s) 393
Eco47I GGWCC 3 cut(s) 478, 1936, 4333
Eco47III AGCGCT 2 cut(s) 520, 3442
Eco52I CGGCCG 1 cut(s) 191
Eco53kI GAGCTC 2 cut(s) 259, 537
Eco57I CTGAAG 3 cut(s) 3676, 4073, 4268
Eco72I CACGTG 4 cut(s) 2429, 2636, 2843, 4108
Eco81I CCTNAGG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 2 cut(s) 259, 537
EcoNI CCTNNNNNAGG 1 cut(s) 1409
EcoO109I RGGNCCY 2 cut(s) 478, 4333
EcoRI GAATTC 1 cut(s) 3700
EcoRII CCWGG 4 cut(s) 163, 2135, 2357, 4146
EcoT22I ATGCAT 1 cut(s) 2003
EcoT38I GRGCYC 3 cut(s) 261, 539, 3607
Esp3I CGTCTC 2 cut(s) 201, 3818
FaqI GGGAC 5 cut(s) 109, 220, 262, 491, 1774
FauI CCCGC 5 cut(s) 287, 468, 501, 4142, 4306
FauNDI CATATG 2 cut(s) 1652, 2050
FbaI TGATCA 2 cut(s) 870, 3390
FblI GTMKAC 1 cut(s) 4081
FokI GGATG 5 cut(s) 355, 616, 1085, 1212, 2019
FriOI GRGCYC 3 cut(s) 261, 539, 3607
GlaI GCGC 6 cut(s) 520, 1289, 3048, 3334, 3442, 3851
GsuI CTGGAG 2 cut(s) 3817, 3863
HaeII RGCGCY 3 cut(s) 522, 3336, 3444
HgaI GACGC 4 cut(s) 222, 476, 604, 1331
HhaI GCGC 6 cut(s) 521, 1290, 3049, 3335, 3443, 3852
Hin1I GRCGYC 2 cut(s) 233, 1323
Hin6I GCGC 6 cut(s) 519, 1288, 3047, 3333, 3441, 3850
HinP1I GCGC 6 cut(s) 519, 1288, 3047, 3333, 3441, 3850
HincII GTYRAC 2 cut(s) 1618, 1803
HindII GTYRAC 2 cut(s) 1618, 1803
HphI GGTGA 6 cut(s) 67, 821, 1141, 1487, 1897, 4222
Hpy166II GTNNAC 6 cut(s) 1095, 1618, 1774, 1803, 1865, 4082
Hpy8I GTNNAC 6 cut(s) 1095, 1618, 1774, 1803, 1865, 4082
Hpy99I CGWCG 3 cut(s) 34, 40, 395
HpyCH4III ACNGT 7 cut(s) 1099, 1622, 1862, 3292, 4169, 4189, 4387
HpyCH4IV ACGT 6 cut(s) 1805, 2428, 2635, 2842, 3310, 4107
HpySE526I ACGT 6 cut(s) 1805, 2428, 2635, 2842, 3310, 4107
Hsp92I GRCGYC 2 cut(s) 233, 1323
HspAI GCGC 6 cut(s) 519, 1288, 3047, 3333, 3441, 3850
KflI GGGWCCC 1 cut(s) 478
KroI GCCGGC 1 cut(s) 189
KroNI GCCGGC 1 cut(s) 191
Ksp22I TGATCA 2 cut(s) 870, 3390
LweI GCATC 7 cut(s) 594, 637, 823, 990, 1234, 1756, 1997
MabI ACCWGGT 1 cut(s) 4146
MaeII ACGT 6 cut(s) 1805, 2428, 2635, 2842, 3310, 4107
MbiI CCGCTC 1 cut(s) 4301
MflI RGATCY 1 cut(s) 3432
MhlI GDGCHC 6 cut(s) 261, 539, 1776, 1867, 3607, 4113
MlsI TGGCCA 2 cut(s) 323, 1751
MluNI TGGCCA 2 cut(s) 323, 1751
MlyI GAGTC 3 cut(s) 491, 603, 649
MmeI TCCRAC 2 cut(s) 546, 2216
Mox20I TGGCCA 2 cut(s) 323, 1751
Mph1103I ATGCAT 1 cut(s) 2003
MroNI GCCGGC 1 cut(s) 189
MroXI GAANNNNTTC 1 cut(s) 3700
MscI TGGCCA 2 cut(s) 323, 1751
MslI CAYNNNNRTG 3 cut(s) 1340, 3162, 3969
Msp20I TGGCCA 2 cut(s) 323, 1751
MspA1I CMGCKG 2 cut(s) 3137, 3278
MspCI CTTAAG 2 cut(s) 1049, 1364
Mva1269I GAATGC 3 cut(s) 3130, 3957, 4375
MvaI CCWGG 4 cut(s) 165, 2137, 2359, 4148
MvnI CGCG 2 cut(s) 106, 3049
NaeI GCCGGC 1 cut(s) 191
NciI CCSGG 7 cut(s) 129, 238, 444, 954, 3325, 4173, 4375
NdeI CATATG 2 cut(s) 1652, 2050
NgoMIV GCCGGC 1 cut(s) 189
NlaIV GGNNCC 6 cut(s) 133, 479, 480, 2162, 3604, 4127
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 6 cut(s) 979, 1475, 1727, 1841, 2843, 3681
NruI TCGCGA 1 cut(s) 106
NsiI ATGCAT 1 cut(s) 2003
NspI RCATGY 4 cut(s) 575, 1513, 2001, 3895
NspV TTCGAA 1 cut(s) 781
OliI CACNNNNGTG 1 cut(s) 3969
PaeI GCATGC 1 cut(s) 3895
PaeR7I CTCGAG 1 cut(s) 260
PagI TCATGA 4 cut(s) 756, 2287, 2494, 2908
PceI AGGCCT 2 cut(s) 251, 2135
PciI ACATGT 1 cut(s) 1509
PcsI WCGNNNNNNNCGW 1 cut(s) 905
PctI GAATGC 3 cut(s) 3130, 3957, 4375
PdiI GCCGGC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 3700
PfeI GAWTC 7 cut(s) 108, 464, 750, 812, 1103, 1736, 2105
PfoI TCCNGGA 2 cut(s) 236, 4171
PleI GAGTC 3 cut(s) 491, 603, 649
PmaCI CACGTG 4 cut(s) 2429, 2636, 2843, 4108
PmlI CACGTG 4 cut(s) 2429, 2636, 2843, 4108
PpsI GAGTC 3 cut(s) 491, 603, 649
Ppu21I YACGTR 5 cut(s) 2429, 2636, 2843, 3311, 4108
PpuMI RGGWCCY 2 cut(s) 478, 4333
PscI ACATGT 1 cut(s) 1509
PsiI TTATAA 2 cut(s) 765, 3902
Psp124BI GAGCTC 2 cut(s) 261, 539
Psp5II RGGWCCY 2 cut(s) 478, 4333
Psp6I CCWGG 4 cut(s) 163, 2135, 2357, 4146
PspCI CACGTG 4 cut(s) 2429, 2636, 2843, 4108
PspGI CCWGG 4 cut(s) 163, 2135, 2357, 4146
PspN4I GGNNCC 6 cut(s) 133, 479, 480, 2162, 3604, 4127
PspPI GGNCC 6 cut(s) 131, 286, 478, 510, 1936, 4333
PspPPI RGGWCCY 2 cut(s) 478, 4333
PspXI VCTCGAGB 1 cut(s) 260
PstI CTGCAG 1 cut(s) 3427
PstNI CAGNNNCTG 1 cut(s) 3841
PsuI RGATCY 1 cut(s) 3432
PvuII CAGCTG 1 cut(s) 3278
RruI TCGCGA 1 cut(s) 106
RsaI GTAC 4 cut(s) 169, 794, 1981, 4457
RsaNI GTAC 4 cut(s) 168, 793, 1980, 4456
RseI CAYNNNNRTG 3 cut(s) 1340, 3162, 3969
SacI GAGCTC 2 cut(s) 261, 539
Sau96I GGNCC 6 cut(s) 131, 286, 478, 510, 1936, 4333
SchI GAGTC 3 cut(s) 491, 603, 649
SduI GDGCHC 6 cut(s) 261, 539, 1776, 1867, 3607, 4113
SexAI ACCWGGT 1 cut(s) 4146
SfaNI GCATC 7 cut(s) 594, 637, 823, 990, 1234, 1756, 1997
SfcI CTRYAG 3 cut(s) 522, 3423, 4401
Sfr274I CTCGAG 1 cut(s) 260
SfuI TTCGAA 1 cut(s) 781
SinI GGWCC 3 cut(s) 478, 1936, 4333
SlaI CTCGAG 1 cut(s) 260
SmiMI CAYNNNNRTG 3 cut(s) 1340, 3162, 3969
SmlI CTYRAG 6 cut(s) 245, 260, 311, 1049, 1364, 3853
SmoI CTYRAG 6 cut(s) 245, 260, 311, 1049, 1364, 3853
SphI GCATGC 1 cut(s) 3895
SseBI AGGCCT 2 cut(s) 251, 2135
SspI AATATT 3 cut(s) 673, 1177, 2233
SstI GAGCTC 2 cut(s) 261, 539
StuI AGGCCT 2 cut(s) 251, 2135
TaaI ACNGT 7 cut(s) 1099, 1622, 1862, 3292, 4169, 4189, 4387
TaiI ACGT 6 cut(s) 1808, 2431, 2638, 2845, 3313, 4110
TaqII GACCGA 1 cut(s) 1689
TatI WGTACW 2 cut(s) 792, 1979
TauI GCSGC 3 cut(s) 189, 233, 378
TfiI GAWTC 7 cut(s) 108, 464, 750, 812, 1103, 1736, 2105
TscAI CASTG 7 cut(s) 151, 454, 586, 1591, 1759, 1776, 3997
TseFI GTSAC 6 cut(s) 979, 1475, 1727, 1841, 2843, 3681
Tsp45I GTSAC 6 cut(s) 979, 1475, 1727, 1841, 2843, 3681
TspGWI ACGGA 6 cut(s) 18, 325, 379, 3098, 3368, 3842
TspRI CASTG 7 cut(s) 151, 454, 586, 1591, 1759, 1776, 3997
Vha464I CTTAAG 2 cut(s) 1049, 1364
VneI GTGCAC 2 cut(s) 1772, 1863
VpaK11BI GGWCC 3 cut(s) 478, 1936, 4333
XagI CCTNNNNNAGG 1 cut(s) 1409
XapI RAATTY 9 cut(s) 137, 291, 1566, 1575, 1816, 1945, 3097, 3147, 3700
XceI RCATGY 4 cut(s) 575, 1513, 2001, 3895
XhoI CTCGAG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 4081
XmnI GAANNNNTTC 1 cut(s) 3700
Zsp2I ATGCAT 1 cut(s) 2003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.