pycom10g27300

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010721
Physical Location & Seq
Reverse (-)
28300942 .. 28303167
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 879 bp
ATGGCCGAAGCTGAGGGAAAATCCGTCGCTCCAAACGCTGACGTTTTGGAATGGCCGAAGAAGGACAAGCGCCGGTTCCTCCACGCTGTGTACCGCGTCGGCGACCTCGATCGCACCATCAAGTTTTATACGGAGGCTTTGGGGATGAAGCTGTTGAGGAAGAGGGACATCCCGGAGGAGAAATACTCGAATGCGTTTCTGGGGTTTGGGCCCGAAGAGTCTCACTTTGTGGTTGAATTGACCTACAATTATGGAGTTTCTTCTTATGATATCGGCACCGGTTTCGGGCACTTCGCCATCGCTACTCCTGATGTTCAAAAACTGGTTGAAGATGTTCGTGCTAAGGGTGGAACTGTGACGAGGGAGCCTGGACCTGTTAAAGGCGGGAAGAGCATCATTGCCTTCGTGAAGGATCCTGATGGTTACATGTTTGAGATTATTCAGCGACCCTCCACTCCTGAGCCACTTTGCCAAGTAATGCTCCGCGTTGGTGATCTTGAACGCTCAATCAAGTTCTATGAGAAGGCATTAGGGTTGAAGCTGTTGAGGACAATTGACAGGCCCGAATACAAGTACACTATAGCCATTTTGGGATATAAAGAAGAAGACCAAACAACTATCTTGGAGTTGACCTATAACTATGGTGTGACCGAATACACCAAAGGAAATGCGTATGCACAGATTGCTATCGGTACTGATGATGTCTACAAAAGTGCTGAGGCTGTCAATTTGGTTACACAAGAGCTCGGAGGAAAGATAACCAGACAGCCAGGACCGATTCCTGGACTCAACACCAAGATCACCTCTTTTCTCGATCCCGACGGCTGGAAAACTGTCCTGGTTGACAACGAAGATTTCTTGAAGGAACTGCAGCCGTAA

Protein Analysis

293

Amino Acids

32.76

Weight (kDa)

5.48

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 25 - 146 2.8e-26 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 28 - 131 4.9e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_6 PF18029 31 - 146 8.8e-06 Glyoxalase-like domain
Glyoxalase PF00903 158 - 277 5.5e-15 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010721)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11840 AT1G11840 AT1G11840 AT1G11840 AT1G11840 AT1G11840
fragaria_vesca FvH4_3g01920
malus_domestica MD05G1348100.v1.1
prunus_persica Prupe.4G019400_v2.0.a1
pyrus_communis pycom05g31560 pycom10g27300
rosa_chinensis RchiOBHm_Chr5g0002821
rosa_laevigata RLG00000031062
rosa_multiflora Rmu_co8395511.1_g000001 Rmu_sc0013603.1_g000013
rosa_roxburghii Rroxscaffold_1G00073030
rosa_rugosa Rorug04G0399900
rosa_samantha Rh5BG025400 Rh5DG023900
rosa_wichuraiana Rw5G002110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AccI GTMKAC 1 cut(s) 705
AccII CGCG 2 cut(s) 96, 486
AciI CCGC 3 cut(s) 94, 384, 484
AclWI GGATC 3 cut(s) 407, 420, 809
AcoI YGGCCR 2 cut(s) 3, 53
AdeI CACNNNGTG 2 cut(s) 88, 229
AfaI GTAC 3 cut(s) 92, 575, 694
AfiI CCNNNNNNNGG 4 cut(s) 285, 380, 782, 825
AflIII ACRYGT 1 cut(s) 426
AgeI ACCGGT 1 cut(s) 278
AgsI TTSAA 6 cut(s) 236, 317, 329, 500, 538, 862
AjnI CCWGG 4 cut(s) 367, 769, 781, 837
AluBI AGCT 4 cut(s) 11, 151, 541, 745
AluI AGCT 4 cut(s) 11, 151, 541, 745
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 3 cut(s) 407, 420, 809
AoxI GGCC 4 cut(s) 3, 53, 209, 560
ApaI GGGCCC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 871
AsiGI ACCGGT 1 cut(s) 278
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 5 cut(s) 209, 210, 371, 561, 773
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 503, 793
AvaII GGWCC 2 cut(s) 371, 773
BaeGI GKGCMC 2 cut(s) 213, 291
BamHI GGATCC 1 cut(s) 412
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 213, 747
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 747
BbvCI CCTCAGC 2 cut(s) 12, 717
BccI CCATC 3 cut(s) 125, 305, 413
BceAI ACGGC 2 cut(s) 838, 859
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 4 cut(s) 369, 771, 783, 839
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 225
BfmI CTRYAG 2 cut(s) 579, 869
BfoI RGCGCY 1 cut(s) 73
BisI GCNGC 1 cut(s) 872
BlsI GCNGC 1 cut(s) 873
Bme1390I CCNGG 5 cut(s) 173, 369, 771, 783, 839
Bme18I GGWCC 2 cut(s) 371, 773
BmgT120I GGNCC 5 cut(s) 209, 210, 371, 561, 773
BmiI GGNNCC 5 cut(s) 77, 211, 277, 366, 414
BmrFI CCNGG 5 cut(s) 173, 369, 771, 783, 839
BmsI GCATC 1 cut(s) 402
BpiI GAAGAC 1 cut(s) 612
BplI GAGNNNNNCTC 2 cut(s) 170, 202
Bpu10I CCTNAGC 4 cut(s) 12, 342, 459, 717
BpuMI CCSGG 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 686
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 380, 782, 825
Bse118I RCCGGY 2 cut(s) 72, 278
Bse1I ACTGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 686
BseBI CCWGG 4 cut(s) 369, 771, 783, 839
BseGI GGATG 2 cut(s) 150, 168
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 4 cut(s) 285, 380, 782, 825
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 450, 708
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 2 cut(s) 213, 291
Bsh1236I CGCG 2 cut(s) 96, 486
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 4 cut(s) 5, 55, 211, 562
BshNI GGYRCC 1 cut(s) 275
BshTI ACCGGT 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 747
BsiSI CCGG 3 cut(s) 73, 173, 279
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 4 cut(s) 285, 380, 782, 825
BsmAI GTCTC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 4 cut(s) 5, 55, 211, 562
Bsp120I GGGCCC 1 cut(s) 209
Bsp1286I GDGCHC 3 cut(s) 213, 291, 747
Bsp143I GATC 5 cut(s) 109, 412, 493, 798, 814
BspACI CCGC 3 cut(s) 94, 384, 484
BspANI GGCC 4 cut(s) 5, 55, 211, 562
BspCNI CTCAG 3 cut(s) 4, 451, 709
BspFNI CGCG 2 cut(s) 96, 486
BspLI GGNNCC 5 cut(s) 77, 211, 277, 366, 414
BspMAI CTGCAG 1 cut(s) 873
BspPI GGATC 3 cut(s) 407, 420, 809
BspQI GCTCTTC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 396
BsrFI RCCGGY 2 cut(s) 72, 278
BsrI ACTGG 1 cut(s) 327
BssAI RCCGGY 2 cut(s) 72, 278
BssMI GATC 5 cut(s) 109, 412, 493, 798, 814
Bst2UI CCWGG 4 cut(s) 369, 771, 783, 839
Bst4CI ACNGT 2 cut(s) 355, 835
Bst6I CTCTTC 3 cut(s) 155, 210, 383
BstAPI GCANNNNNTGC 1 cut(s) 683
BstDEI CTNAG 4 cut(s) 12, 342, 459, 717
BstENI CCTNNNNNAGG 1 cut(s) 378
BstF5I GGATG 2 cut(s) 150, 168
BstFNI CGCG 2 cut(s) 96, 486
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 5 cut(s) 112, 415, 496, 801, 817
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 5 cut(s) 109, 412, 493, 798, 814
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 3 cut(s) 35, 390, 683
BstNI CCWGG 4 cut(s) 369, 771, 783, 839
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 5 cut(s) 171, 367, 769, 781, 837
BstSFI CTRYAG 2 cut(s) 579, 869
BstSLI GKGCMC 2 cut(s) 213, 291
BstUI CGCG 2 cut(s) 96, 486
BstV2I GAAGAC 1 cut(s) 612
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuRI GGCC 4 cut(s) 5, 55, 211, 562
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 2 cut(s) 150, 168
CfoI GCGC 1 cut(s) 72
Cfr10I RCCGGY 2 cut(s) 72, 278
Cfr13I GGNCC 5 cut(s) 209, 210, 371, 561, 773
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 91, 574, 693
CspAI ACCGGT 1 cut(s) 278
CviAII CATG 1 cut(s) 427
CviQI GTAC 3 cut(s) 91, 574, 693
DdeI CTNAG 4 cut(s) 12, 342, 459, 717
DpnI GATC 5 cut(s) 111, 414, 495, 800, 816
DpnII GATC 5 cut(s) 109, 412, 493, 798, 814
DraIII CACNNNGTG 2 cut(s) 88, 229
EaeI YGGCCR 2 cut(s) 3, 53
Eam1104I CTCTTC 3 cut(s) 155, 210, 383
EarI CTCTTC 3 cut(s) 155, 210, 383
Ecl136II GAGCTC 1 cut(s) 745
Eco24I GRGCYC 2 cut(s) 213, 747
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 371, 773
Eco53kI GAGCTC 1 cut(s) 745
EcoICRI GAGCTC 1 cut(s) 745
EcoNI CCTNNNNNAGG 1 cut(s) 378
EcoRII CCWGG 4 cut(s) 367, 769, 781, 837
EcoRV GATATC 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 213, 747
FaeI CATG 1 cut(s) 430
FaqI GGGAC 1 cut(s) 179
FatI CATG 1 cut(s) 426
FauI CCCGC 1 cut(s) 377
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 1 cut(s) 872
FokI GGATG 2 cut(s) 155, 157
FriOI GRGCYC 2 cut(s) 213, 747
Fsp4HI GCNGC 1 cut(s) 872
GlaI GCGC 1 cut(s) 71
GluI GCNGC 1 cut(s) 872
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 4 cut(s) 5, 55, 211, 562
HapII CCGG 3 cut(s) 73, 173, 279
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 72
Hin1II CATG 1 cut(s) 430
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 2 cut(s) 630, 844
HindII GTYRAC 2 cut(s) 630, 844
HinfI GANTC 3 cut(s) 218, 778, 786
HpaII CCGG 3 cut(s) 73, 173, 279
HphI GGTGA 2 cut(s) 503, 793
Hpy166II GTNNAC 5 cut(s) 91, 576, 630, 706, 844
Hpy188I TCNGA 1 cut(s) 749
Hpy188III TCNNGA 8 cut(s) 308, 406, 416, 458, 497, 812, 818, 859
Hpy8I GTNNAC 5 cut(s) 91, 576, 630, 706, 844
Hpy99I CGWCG 3 cut(s) 29, 101, 824
HpyAV CCTTC 5 cut(s) 55, 403, 412, 517, 856
HpyCH4III ACNGT 2 cut(s) 355, 835
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 2 cut(s) 677, 871
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 390, 683
HpyF3I CTNAG 4 cut(s) 12, 342, 459, 717
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 1 cut(s) 430
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 5 cut(s) 109, 412, 493, 798, 814
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 3 cut(s) 34, 364, 486
LweI GCATC 1 cut(s) 402
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 4 cut(s) 355, 422, 646, 733
MalI GATC 5 cut(s) 111, 414, 495, 800, 816
MboI GATC 5 cut(s) 109, 412, 493, 798, 814
MboII GAAGA 9 cut(s) 70, 172, 227, 252, 341, 400, 614, 617, 863
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 3 cut(s) 213, 291, 747
MluCI AATT 4 cut(s) 236, 247, 552, 727
MlyI GAGTC 2 cut(s) 227, 780
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 1 cut(s) 378
MspI CCGG 3 cut(s) 73, 173, 279
MspR9I CCNGG 5 cut(s) 173, 369, 771, 783, 839
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 196
MvaI CCWGG 4 cut(s) 369, 771, 783, 839
MvnI CGCG 2 cut(s) 96, 486
MwoI GCNNNNNNNGC 3 cut(s) 35, 390, 683
NciI CCSGG 1 cut(s) 173
NdeII GATC 5 cut(s) 109, 412, 493, 798, 814
NlaIII CATG 1 cut(s) 430
NlaIV GGNNCC 5 cut(s) 77, 211, 277, 366, 414
NmuCI GTSAC 2 cut(s) 355, 646
NspI RCATGY 1 cut(s) 430
PciI ACATGT 1 cut(s) 426
PciSI GCTCTTC 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 778
PfoI TCCNGGA 2 cut(s) 171, 781
PinAI ACCGGT 1 cut(s) 278
PkrI GCNGC 1 cut(s) 873
Ple19I CGATCG 1 cut(s) 112
PleI GAGTC 2 cut(s) 226, 780
PpsI GAGTC 2 cut(s) 226, 780
PscI ACATGT 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 747
Psp6I CCWGG 4 cut(s) 367, 769, 781, 837
PspGI CCWGG 4 cut(s) 367, 769, 781, 837
PspN4I GGNNCC 5 cut(s) 77, 211, 277, 366, 414
PspOMI GGGCCC 1 cut(s) 209
PspPI GGNCC 5 cut(s) 209, 210, 371, 561, 773
PstI CTGCAG 1 cut(s) 873
PsuI RGATCY 1 cut(s) 412
PvuI CGATCG 1 cut(s) 112
RsaI GTAC 3 cut(s) 92, 575, 694
RsaNI GTAC 3 cut(s) 91, 574, 693
SacI GAGCTC 1 cut(s) 747
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 1 cut(s) 872
Sau3AI GATC 5 cut(s) 109, 412, 493, 798, 814
Sau96I GGNCC 5 cut(s) 209, 210, 371, 561, 773
SchI GAGTC 2 cut(s) 227, 780
ScrFI CCNGG 5 cut(s) 173, 369, 771, 783, 839
SduI GDGCHC 3 cut(s) 213, 291, 747
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 2 cut(s) 579, 869
SinI GGWCC 2 cut(s) 371, 773
Sse9I AATT 4 cut(s) 236, 247, 552, 727
SsiI CCGC 3 cut(s) 94, 384, 484
SstI GAGCTC 1 cut(s) 747
StyD4I CCNGG 5 cut(s) 171, 367, 769, 781, 837
TaaI ACNGT 2 cut(s) 355, 835
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 108, 188, 813
TaqII GACCGA 2 cut(s) 665, 790
TasI AATT 4 cut(s) 236, 247, 552, 727
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 1 cut(s) 778
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TseFI GTSAC 2 cut(s) 355, 646
TseI GCWGC 1 cut(s) 871
Tsp45I GTSAC 2 cut(s) 355, 646
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 2 cut(s) 13, 146
VpaK11BI GGWCC 2 cut(s) 371, 773
XagI CCTNNNNNAGG 1 cut(s) 378
XceI RCATGY 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 705
XmnI GAANNNNTTC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.