Rh5DG023900

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010721
Physical Location & Seq
Forward (+)
1673999 .. 1675980
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG023900.1

Sequence Viewer

Length: 759 bp
ATGGCCGAGACTGAACCCAAATCCGCGGCTCCGGAGCTTCTGGAATGGCCGAAGAAGGATAAGCGCCGCCTCCTCCACGCCGTCTACCGCGTCGGCGATCTCGAGCGCACCATCAAGTTTTACACTGAGGCTCTGGGGATGAAGCTGTTGCGGCAGAGGGATATTCCGGAAGAGAAGTATTCCAATGCTTTCCTCGGGTTCGGGCCCGAAGAAACGAACTTCGTAGTGGAGCTCACTTATAACTACGGAGTCACTTCCTATGATATTGGGACTGGATTTGGACATTTCGCTATCGCTACTCCGGATGTTTACAAATTGGTGGAGGATATTCGTGCCAAGGGTGGGAATGTGACTAGGGAGCCTGGTCCGGTTAAAGGTGGGAACAGTGTCATTGCCTTTGTGAAGGATCCTGATGGTTACACGTTTGAGCTTATTCAGAGACCTTCAACCCCTGAGCCGCTTTGTCAGATTATGCTTCGAGTTGGTGATCTTCAGCGCTCAATCAGCTTCTATGAAAGGGCACTAGGGTTGAAGCTTTTAAGGAGGATTGAGAGACCTGAGTACAAGTACAACATAGCTATCCTGGGATATGCTGAAGAAGACCAGACAACTATTTTGGAGCTGACTTATAACTATGGTGTGACCGAATATACCAAGGGGAATGCGTATGCACAGATAAATCTCGAAATCATTTTAACATTTATGAGCAGAGGAGTGGTCCTGGTTGACAATGTGGACTTTCTCAAGGAACTGCAGTAA

Protein Analysis

252

Amino Acids

28.71

Weight (kDa)

5.33

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 23 - 144 1.1e-26 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 26 - 129 1.1e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 156 - 221 1.1e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010721)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11840 AT1G11840 AT1G11840 AT1G11840 AT1G11840 AT1G11840
fragaria_vesca FvH4_3g01920
malus_domestica MD05G1348100.v1.1
prunus_persica Prupe.4G019400_v2.0.a1
pyrus_communis pycom05g31560 pycom10g27300
rosa_chinensis RchiOBHm_Chr5g0002821
rosa_laevigata RLG00000031062
rosa_multiflora Rmu_co8395511.1_g000001 Rmu_sc0013603.1_g000013
rosa_roxburghii Rroxscaffold_1G00073030
rosa_rugosa Rorug04G0399900
rosa_samantha Rh5BG025400 Rh5DG023900
rosa_wichuraiana Rw5G002110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 240, 630
AccI GTMKAC 1 cut(s) 84
AccII CGCG 2 cut(s) 26, 90
AccIII TCCGGA 3 cut(s) 31, 166, 301
AciI CCGC 6 cut(s) 24, 26, 67, 88, 151, 458
AclWI GGATC 2 cut(s) 401, 414
AcoI YGGCCR 2 cut(s) 3, 47
AcuI CTGAAG 2 cut(s) 476, 615
AfaI GTAC 2 cut(s) 563, 569
AfeI AGCGCT 1 cut(s) 497
AfiI CCNNNNNNNGG 2 cut(s) 342, 374
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 2 cut(s) 447, 532
AjnI CCWGG 3 cut(s) 361, 582, 720
AluBI AGCT 8 cut(s) 37, 145, 232, 430, 507, 535, 578, 622
AluI AGCT 8 cut(s) 37, 145, 232, 430, 507, 535, 578, 622
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 3 cut(s) 2, 433, 547
AlwI GGATC 2 cut(s) 401, 414
Ama87I CYCGRG 2 cut(s) 101, 194
Aor13HI TCCGGA 3 cut(s) 31, 166, 301
Aor51HI AGCGCT 1 cut(s) 497
AoxI GGCC 3 cut(s) 3, 47, 203
ApaI GGGCCC 1 cut(s) 207
ArsI GACNNNNNNTTYG 2 cut(s) 598, 630
AspLEI GCGC 3 cut(s) 66, 108, 498
AspS9I GGNCC 4 cut(s) 203, 204, 365, 718
AsuHPI GGTGA 1 cut(s) 497
AvaI CYCGRG 2 cut(s) 101, 194
AvaII GGWCC 2 cut(s) 365, 718
BaeGI GKGCMC 2 cut(s) 207, 523
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 2 cut(s) 207, 234
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 234
BccI CCATC 2 cut(s) 119, 407
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 3 cut(s) 363, 584, 722
BcoDI GTCTC 3 cut(s) 2, 433, 547
BfaI CTAG 2 cut(s) 354, 524
BfmI CTRYAG 1 cut(s) 752
BfoI RGCGCY 2 cut(s) 67, 499
BisI GCNGC 4 cut(s) 27, 67, 152, 458
BlsI GCNGC 4 cut(s) 28, 68, 153, 459
Bme1390I CCNGG 3 cut(s) 363, 584, 722
Bme18I GGWCC 2 cut(s) 365, 718
BmeT110I CYCGRG 2 cut(s) 101, 194
BmgT120I GGNCC 4 cut(s) 203, 204, 365, 718
BmiI GGNNCC 4 cut(s) 30, 205, 360, 408
BmrFI CCNGG 3 cut(s) 363, 584, 722
BpiI GAAGAC 1 cut(s) 606
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 728
BsaI GGTCTC 2 cut(s) 433, 547
BsaJI CCNNGG 5 cut(s) 24, 193, 336, 583, 654
BsaWI WCCGGW 4 cut(s) 31, 166, 301, 367
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 374
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 390
BseAI TCCGGA 3 cut(s) 31, 166, 301
BseBI CCWGG 3 cut(s) 363, 584, 722
BseDI CCNNGG 5 cut(s) 24, 193, 336, 583, 654
BseGI GGATG 2 cut(s) 144, 310
BseLI CCNNNNNNNGG 2 cut(s) 342, 374
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 3 cut(s) 117, 444, 549
BseNI ACTGG 1 cut(s) 277
BseRI GAGGAG 2 cut(s) 62, 726
BseSI GKGCMC 2 cut(s) 207, 523
Bsh1236I CGCG 2 cut(s) 26, 90
BshFI GGCC 3 cut(s) 5, 49, 205
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 2 cut(s) 101, 194
BsiSI CCGG 4 cut(s) 32, 167, 302, 368
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 2 cut(s) 342, 374
BsmAI GTCTC 3 cut(s) 2, 433, 547
BsmFI GGGAC 1 cut(s) 283
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 3 cut(s) 5, 49, 205
Bso31I GGTCTC 2 cut(s) 433, 547
BsoBI CYCGRG 2 cut(s) 101, 194
Bsp120I GGGCCC 1 cut(s) 203
Bsp1286I GDGCHC 3 cut(s) 207, 234, 523
Bsp13I TCCGGA 3 cut(s) 31, 166, 301
Bsp143I GATC 3 cut(s) 97, 406, 487
BspACI CCGC 6 cut(s) 24, 26, 67, 88, 151, 458
BspANI GGCC 3 cut(s) 5, 49, 205
BspCNI CTCAG 3 cut(s) 118, 445, 550
BspEI TCCGGA 3 cut(s) 31, 166, 301
BspFNI CGCG 2 cut(s) 26, 90
BspLI GGNNCC 4 cut(s) 30, 205, 360, 408
BspMAI CTGCAG 1 cut(s) 756
BspPI GGATC 2 cut(s) 401, 414
BspTNI GGTCTC 2 cut(s) 433, 547
BsrDI GCAATG 1 cut(s) 390
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 5 cut(s) 24, 193, 336, 583, 654
BssMI GATC 3 cut(s) 97, 406, 487
BssT1I CCWWGG 2 cut(s) 336, 654
Bst2UI CCWGG 3 cut(s) 363, 584, 722
Bst4CI ACNGT 1 cut(s) 386
Bst6I CTCTTC 1 cut(s) 165
BstDEI CTNAG 3 cut(s) 126, 453, 558
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 144, 310
BstFNI CGCG 2 cut(s) 26, 90
BstH2I RGCGCY 2 cut(s) 67, 499
BstHHI GCGC 3 cut(s) 66, 108, 498
BstKTI GATC 3 cut(s) 100, 409, 490
BstMAI GTCTC 3 cut(s) 2, 433, 547
BstMBI GATC 3 cut(s) 97, 406, 487
BstMWI GCNNNNNNNGC 2 cut(s) 151, 504
BstNI CCWGG 3 cut(s) 363, 584, 722
BstSCI CCNGG 3 cut(s) 361, 582, 720
BstSFI CTRYAG 1 cut(s) 752
BstSLI GKGCMC 2 cut(s) 207, 523
BstUI CGCG 2 cut(s) 26, 90
BstV2I GAAGAC 1 cut(s) 606
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 3 cut(s) 5, 49, 205
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 2 cut(s) 144, 310
BtsIMutI CAGTG 2 cut(s) 123, 391
CfoI GCGC 3 cut(s) 66, 108, 498
Cfr13I GGNCC 4 cut(s) 203, 204, 365, 718
Cfr42I CCGCGG 1 cut(s) 27
CseI GACGC 1 cut(s) 79
Csp6I GTAC 2 cut(s) 562, 568
CviQI GTAC 2 cut(s) 562, 568
DdeI CTNAG 3 cut(s) 126, 453, 558
DpnI GATC 3 cut(s) 99, 408, 489
DpnII GATC 3 cut(s) 97, 406, 487
EaeI YGGCCR 2 cut(s) 3, 47
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 2 cut(s) 336, 654
Eco24I GRGCYC 2 cut(s) 207, 234
Eco31I GGTCTC 2 cut(s) 433, 547
Eco47I GGWCC 2 cut(s) 365, 718
Eco47III AGCGCT 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 476, 615
Eco88I CYCGRG 2 cut(s) 101, 194
EcoICRI GAGCTC 1 cut(s) 232
EcoRII CCWGG 3 cut(s) 361, 582, 720
EcoT14I CCWWGG 2 cut(s) 336, 654
EcoT38I GRGCYC 2 cut(s) 207, 234
ErhI CCWWGG 2 cut(s) 336, 654
FaqI GGGAC 1 cut(s) 283
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 4 cut(s) 27, 67, 152, 458
FokI GGATG 2 cut(s) 151, 317
FriOI GRGCYC 2 cut(s) 207, 234
Fsp4HI GCNGC 4 cut(s) 27, 67, 152, 458
FspBI CTAG 2 cut(s) 354, 524
GlaI GCGC 3 cut(s) 65, 107, 497
GluI GCNGC 4 cut(s) 27, 67, 152, 458
HaeII RGCGCY 2 cut(s) 67, 499
HaeIII GGCC 3 cut(s) 5, 49, 205
HapII CCGG 4 cut(s) 32, 167, 302, 368
HgaI GACGC 1 cut(s) 79
HhaI GCGC 3 cut(s) 66, 108, 498
Hin6I GCGC 3 cut(s) 64, 106, 496
HinP1I GCGC 3 cut(s) 64, 106, 496
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 1 cut(s) 249
HpaII CCGG 4 cut(s) 32, 167, 302, 368
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 4 cut(s) 85, 310, 727, 736
Hpy188I TCNGA 2 cut(s) 438, 468
Hpy188III TCNNGA 7 cut(s) 32, 41, 101, 167, 302, 410, 683
Hpy8I GTNNAC 4 cut(s) 85, 310, 727, 736
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 3 cut(s) 49, 397, 453
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 2 cut(s) 671, 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 504
HpyF3I CTNAG 3 cut(s) 126, 453, 558
HpySE526I ACGT 1 cut(s) 422
HspAI GCGC 3 cut(s) 64, 106, 496
Kpn2I TCCGGA 3 cut(s) 31, 166, 301
KspI CCGCGG 1 cut(s) 27
Kzo9I GATC 3 cut(s) 97, 406, 487
LmnI GCTCC 5 cut(s) 34, 34, 229, 358, 619
MaeI CTAG 2 cut(s) 354, 524
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 4 cut(s) 250, 349, 416, 640
MalI GATC 3 cut(s) 99, 408, 489
MboI GATC 3 cut(s) 97, 406, 487
MboII GAAGA 6 cut(s) 64, 182, 221, 482, 608, 611
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 3 cut(s) 207, 234, 523
MluCI AATT 1 cut(s) 314
MlyI GAGTC 1 cut(s) 258
MnlI CCTC 8 cut(s) 80, 83, 121, 150, 203, 316, 537, 704
MroI TCCGGA 3 cut(s) 31, 166, 301
MseI TTAA 3 cut(s) 372, 539, 695
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 4 cut(s) 32, 167, 302, 368
MspR9I CCNGG 3 cut(s) 363, 584, 722
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 3 cut(s) 363, 584, 722
MvnI CGCG 2 cut(s) 26, 90
MwoI GCNNNNNNNGC 2 cut(s) 151, 504
NdeII GATC 3 cut(s) 97, 406, 487
NlaIV GGNNCC 4 cut(s) 30, 205, 360, 408
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 3 cut(s) 250, 349, 640
PaeR7I CTCGAG 1 cut(s) 101
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 667
PkrI GCNGC 4 cut(s) 28, 68, 153, 459
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PsiI TTATAA 2 cut(s) 240, 630
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 361, 582, 720
PspGI CCWGG 3 cut(s) 361, 582, 720
PspN4I GGNNCC 4 cut(s) 30, 205, 360, 408
PspOMI GGGCCC 1 cut(s) 203
PspPI GGNCC 4 cut(s) 203, 204, 365, 718
PstI CTGCAG 1 cut(s) 756
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 2 cut(s) 563, 569
RsaNI GTAC 2 cut(s) 562, 568
SacI GAGCTC 1 cut(s) 234
SacII CCGCGG 1 cut(s) 27
SaqAI TTAA 3 cut(s) 372, 539, 695
SatI GCNGC 4 cut(s) 27, 67, 152, 458
Sau3AI GATC 3 cut(s) 97, 406, 487
Sau96I GGNCC 4 cut(s) 203, 204, 365, 718
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 3 cut(s) 363, 584, 722
SduI GDGCHC 3 cut(s) 207, 234, 523
SfcI CTRYAG 1 cut(s) 752
Sfr274I CTCGAG 1 cut(s) 101
Sfr303I CCGCGG 1 cut(s) 27
SgrBI CCGCGG 1 cut(s) 27
SinI GGWCC 2 cut(s) 365, 718
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 101, 743
SmoI CTYRAG 2 cut(s) 101, 743
Sse9I AATT 1 cut(s) 314
SsiI CCGC 6 cut(s) 24, 26, 67, 88, 151, 458
SspMI CTAG 2 cut(s) 354, 524
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 3 cut(s) 361, 582, 720
StyI CCWWGG 2 cut(s) 336, 654
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 1 cut(s) 425
TaqI TCGA 3 cut(s) 102, 478, 684
TaqII GACCGA 1 cut(s) 659
TasI AATT 1 cut(s) 314
TatI WGTACW 2 cut(s) 561, 567
TauI GCSGC 4 cut(s) 29, 69, 154, 460
Tru1I TTAA 3 cut(s) 372, 539, 695
Tru9I TTAA 3 cut(s) 372, 539, 695
TscAI CASTG 2 cut(s) 130, 391
TseFI GTSAC 3 cut(s) 250, 349, 640
Tsp45I GTSAC 3 cut(s) 250, 349, 640
TspDTI ATGAA 2 cut(s) 155, 528
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 2 cut(s) 130, 391
VpaK11BI GGWCC 2 cut(s) 365, 718
XhoI CTCGAG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 2 cut(s) 354, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.