Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0014382

Physical Location & Seq

Genomic Coordinates Reverse (-)

28459947 .. 28466304

Gene Structure 6358 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3105 bp

ATGAACTTTAGGAGAGAACTTACACTTTTCGCATGCTATAGTTACCCAACAAACCAAAACGAGCGCCAAACAGCGGTCTCGCACTCACAGCACGTTATCTCATCCACTCTTTTCAGCCACTCTCATTCTTCTTCTTCCTCTTCTTCTTCTTCTTCAGCTCGATGCGAAATGAGTTCTTCTCCTCACGCACTACTCCATCTCCAACCCCGACCTCCTCCGTCGCCCCCCTTATGCTTCGGCTCGATTCCGTTTTCTTCGCAATCACTCACCCAATTCAAGAAATTCAAAAAAAGCAACTTTTCTCTGAAGTTGTACAATAACAGCTCCAAAAGCGGCGTTTTCGGTAGAACTAGCGGCCTAATCAGAAGCTCGGTGGCCGAGGAATCGAATGGTGCGGCGGAGCAGAAGCCGAAGCAGCAAGCCGTGGTGAAGAGGCCCTACCCTTTCCACGAAATCGAGCTCAAATGGCAGTGTTATTGGGAGGAAAATCGGACGTTTCGAACCCCCGATGAAATTGATACCTCCAAGCCCAAATACTATGTTCTTGACATGTTCCCGTATCCCAGCGGAGCTGGGTTACATGTTGGCCATCCACTTGGATATACTGCCACGGACATTCTTGCTAGGCTCAAACGCATGCAGGGTTACAATGTGTTACACCCAATGGGATGGGATGCATTTGGATTGCCAGCGGAGCAATATGCAATAGAGACAGGAACCCACCCGAAGACCACAACCTTGAAGAACATTGATCGCTTTCGCTCCCAGCTTAAATCATTAGGATTCTCATATGACTGGGATCGTGAAATTTCTACAACAGAACCAGAATATTATAGATGGACCCAATGGATCTTTCTTCAGCTATTACAGCGAGGATTGGCGTATCAGGCTGAAGTGCCAGTCAATTGGTGCCCTGCTCTCGGTACTGTCTTGGCAAATGAAGAGGTGATAGATGGTTTGAGTGAGCGTGGGGGTCATCCAGTTATAAGAAAGCCAATGAAGCAATGGATGCTCAAGATCACTGCATATGCTGATCGTCTTCTAGACGATTTAGATGACCTTGACTGGCCTGAAAGCATAAAAGAAATGCAAAGAAACTGGATAGGGAGGTCAGAAGGTGCTGAGATGGAATTTCCTATTCTTAGCAGTGATGGACAGGAAAGAGACACAAAGATTACAATCTATACTACCAGGCCTGACACCATATTTGGAGCAACCTATTTAGTCGTGGCACCCGAGCATCCCTTGTTGTCAACGTTAGTATCAACAGCTCAGAGAGAAACTGTGGAGGAGTACATCGATCTTGCCTCAAGAAAGAGTGACCTTGAGAGGACTGAGCTTCAGAAGGAGAAAACCGGGGTCTTCAGTGGTTGCTATGCTAAAAATCCTGTTAATGGGGAAGCTGTTCCTATATGGGTTGCGGACTATGTCTTGGGGAGTTATGGAACAGGGGCAATTATGGCTGTTCCTGCACATGACACTCGTGATTTTGAGTTTGCATCAAAATTTGACATCCCAATTCGTTGGGTTGTGATGCCGGATGATAAAAATATCAGTGGTTCCAAAAAGGCCTATTCAGGTGAAGGTACTGTTATAAATTCTTCAAATTCAACACTGGGGCTGGACATTAATGACTTATCTAGCAAAGAAGCTGCTTCCAAAGTCATTGACTGGGCTGAGAAAACTGCACATGGGAAGAAAAAGGTGAACTTTAAGTTAAGGGATTGGCTTTTTGCTCGGCAACGTTATTGGGGTGAACCTATTCCAGTAGTTTTTTTGGATGATACTGGTGAGACTGTTCCCATACTTGAAACTGAACTGCCCCTTACCCTACCAGAACTGGATGATTTTTCTCCCACTGGGACAGGAGAACCACCACTATCAAAAGCAGTTTCTTGGGTTAAAACTAAGGACCCTTTATCTGGAAAACCAGCTAGACGAGAGACAAGCACGATGCCTCAATGGGCTGGTTCTTGCTGGTACTATTTGAGATTTATGGACCCAAAAAATTCAGAAGAATTGGTGGAAAAGAGGAAAGAAAGGTACTGGAACCAAGTTGATGTGTATGTTGGGGGTGCAGAACATGCTGTTCTTCATTTACTTTATTCAAGGTTCTGGCACAAGGTTCTCTATGACATTGGCGCTGTATCCACCAAAGAACCTTTCAAGTGTGTCATAAACCAGGGCATTATTCTTGGCGAAGTTCAATATATCGCTTACAAGGACTCTGATGGGAATTTTATCTCTGCCGACTCTGGGACAGCAGTGGAACATCAGCAAGAAATAATACCAGAAGAAAAGGTCATGAAATCGGGGGATTCTTTTGTACTGAAAGACAATCCTGACATTTGTCTGATTGCACGCTCTCATAAAATGAGTAAAAGCAGGGGAAATGTAGTCAATCCCGATGATGTTGTCTCTGAGTACGGGGCGGATTCACTTCGCTTATATGAAATGTTCATGGGGCCACTTAGAGATTCAAAGACCTGGAATACGAGTGGAATTGAAGGTGTCCATCGATTTTTGGGAAGAACATGGAGGCTAGTCGTTGGTTCACCATTGTCTGATGGTACTATCAAGGATGGGACAGTAGTGACTGATGGGGAAGCTACGTTGGAGCAACTTCGTTCTCTCCATAAATGCATTGCAAAGGTAACCGAAGAAATAGAAGCAACAAGGTTCAACACTGGAATATCTGCGATGATGGAATTCCTTAATGCGGCTTATAAGTGGCAAAACCATCCAAGATTGGTTATTGAAGCATTTGTTTTGTTGCTCTCACCGTATGCACCTCATATGGCCGAGGAGCTTTGGTTTCGCTTGGGACACTCCAAATCTCTGGCATATGAGCCTTTTCCGAAGGCGGATCCTGCTTACTTGAAGGAATCTACTATTGTCCTCCCAGTTCAGATCAATGGCAAGACCAGGGGTACGATCCAGGTTGAAGAGACCTGTTCGGAGGAGGATGCTTTCCAACTAGCAACACAAGATGAAAAACTCTCCAAATATCTGGATGGAAAGTCGATTAAAAAGAGAATTTTTGTCCCTGGCAAGATTCTGAATGTCATCCTGGACCGTGAAAACGTCAAGGCAGCTGCCCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

1035

Amino Acids

116.69

Weight (kDa)

6.2

Isoelectric Point (pI)

40.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	156 - 368	5.5e-29	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	184 - 316	7e-18	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	365 - 559	2.5e-63	Leucyl-tRNA synthetase, editing domain
tRNA-synt_1	PF00133	789 - 819	8.5e-07	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	875 - 982	1.5e-17	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04350
fragaria_vesca	FvH4_3g01570 FvH4_3g01570
malus_domestica	MD10G1325600.v1.1
prunus_persica	Prupe.4G016400_v2.0.a1 Prupe.4G016400_v2.0.a1
pyrus_communis	pycom10g27530
rosa_chinensis	RchiOBHm_Chr5g0002381
rosa_laevigata	RLG00000031024
rosa_multiflora	Rmu_sc0006137.1_g000003 Rmu_sc0017945.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073490
rosa_rugosa	Rorug04G0395800.1
rosa_samantha	Rh5AG018500 Rh5BG021200 Rh5DG019500
rosa_wichuraiana	Rw5G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	986, 1595, 2727
AccB1I	GGYRCC	2 cut(s)	909, 1231
AclI	AACGTT	2 cut(s)	1256, 1744
AclWI	GGATC	5 cut(s)	807, 857, 2861, 2874, 2929
AcoI	YGGCCR	3 cut(s)	375, 586, 2799
AcuI	CTGAAG	6 cut(s)	138, 326, 842, 912, 1325, 1348
AfiI	CCNNNNNNNGG	6 cut(s)	73, 920, 1394, 1922, 2256, 2719
AflIII	ACRYGT	2 cut(s)	549, 580
AjnI	CCWGG	7 cut(s)	1190, 2181, 2486, 2924, 2937, 3046, 3069
AjuI	GAANNNNNNNTTGG	4 cut(s)	837, 869, 2147, 2179
Alw21I	GWGCWC	1 cut(s)	462
Alw26I	GTCTC	7 cut(s)	82, 704, 1158, 1787, 1937, 2422, 2942
AlwI	GGATC	5 cut(s)	807, 857, 2861, 2874, 2929
Ama87I	CYCGRG	1 cut(s)	1235
AoxI	GGCC	9 cut(s)	355, 375, 434, 586, 1067, 1193, 1569, 2465, 2799
ApeKI	GCWGC	4 cut(s)	415, 1652, 3092, 3095
ArsI	GACNNNNNNTTYG	2 cut(s)	1190, 1222
AseI	ATTAAT	1 cut(s)	1629
Asp700I	GAANNNNTTC	2 cut(s)	1404, 1761
AspLEI	GCGC	2 cut(s)	66, 2144
AspS9I	GGNCC	6 cut(s)	435, 840, 1912, 1999, 2465, 3073
AsuC2I	CCSGG	1 cut(s)	1357
AsuHPI	GGTGA	9 cut(s)	259, 439, 958, 1592, 1717, 1766, 1802, 2547, 2772
AsuII	TTCGAA	1 cut(s)	499
AvaI	CYCGRG	1 cut(s)	1235
AvaII	GGWCC	4 cut(s)	840, 1912, 1999, 3073
BaeGI	GKGCMC	1 cut(s)	914
BaeI	ACNNNNGTAYC	2 cut(s)	1245, 1278
BalI	TGGCCA	1 cut(s)	588
BamHI	GGATCC	1 cut(s)	2866
BanI	GGYRCC	2 cut(s)	909, 1231
BanII	GRGCYC	1 cut(s)	462
BarI	GAAGNNNNNNTAC	2 cut(s)	422, 454
BauI	CACGAG	1 cut(s)	1482
BbsI	GAAGAC	3 cut(s)	734, 1031, 1354
Bbv12I	GWGCWC	1 cut(s)	462
BbvI	GCAGC	3 cut(s)	427, 1639, 3082
BceAI	ACGGC	1 cut(s)	407
BciT130I	CCWGG	7 cut(s)	1192, 2183, 2488, 2926, 2939, 3048, 3071
BciVI	GTATCC	2 cut(s)	570, 2158
BcnI	CCSGG	1 cut(s)	1357
BcoDI	GTCTC	7 cut(s)	82, 704, 1158, 1787, 1937, 2422, 2942
BfaI	CTAG	7 cut(s)	351, 624, 1043, 1641, 1935, 2543, 2978
BfmI	CTRYAG	1 cut(s)	37
BfoI	RGCGCY	2 cut(s)	67, 2145
BfuI	GTATCC	2 cut(s)	570, 2158
BisI	GCNGC	8 cut(s)	334, 355, 396, 416, 1653, 2721, 3093, 3096
BlsI	GCNGC	8 cut(s)	335, 356, 397, 417, 1654, 2722, 3094, 3097
Bme1390I	CCNGG	8 cut(s)	1192, 1357, 2183, 2488, 2926, 2939, 3048, 3071
Bme18I	GGWCC	4 cut(s)	840, 1912, 1999, 3073
BmeT110I	CYCGRG	1 cut(s)	1235
BmgT120I	GGNCC	6 cut(s)	435, 840, 1912, 1999, 2465, 3073
BmrFI	CCNGG	8 cut(s)	1192, 1357, 2183, 2488, 2926, 2939, 3048, 3071
BmrI	ACTGGG	5 cut(s)	805, 1625, 1681, 1869, 2897
BmsI	GCATC	8 cut(s)	152, 664, 999, 1249, 1508, 1524, 1944, 2956
BmuI	ACTGGG	5 cut(s)	805, 1625, 1681, 1869, 2897
BoxI	GACNNNNGTC	1 cut(s)	2349
BpiI	GAAGAC	3 cut(s)	734, 1031, 1354
BplI	GAGNNNNNCTC	2 cut(s)	163, 195
Bpu14I	TTCGAA	1 cut(s)	499
BpuEI	CTTGAG	3 cut(s)	998, 1294, 1346
BpuMI	CCSGG	1 cut(s)	1357
Bsa29I	ATCGAT	2 cut(s)	1299, 2518
BsaBI	GATNNNNATC	1 cut(s)	1178
BsaI	GGTCTC	2 cut(s)	82, 2942
BsaJI	CCNNGG	8 cut(s)	378, 423, 609, 1356, 2182, 2802, 2925, 3046
BsaXI	ACNNNNNCTCC	4 cut(s)	183, 213, 2798, 2828
Bsc4I	CCNNNNNNNGG	6 cut(s)	73, 920, 1394, 1922, 2256, 2719
Bse3DI	GCAATG	2 cut(s)	1010, 2643
Bse8I	GATNNNNATC	1 cut(s)	1178
BseBI	CCWGG	7 cut(s)	1192, 2183, 2488, 2926, 2939, 3048, 3071
BseCI	ATCGAT	2 cut(s)	1299, 2518
BseDI	CCNNGG	8 cut(s)	378, 423, 609, 1356, 2182, 2802, 2925, 3046
BseJI	GATNNNNATC	1 cut(s)	1178
BseLI	CCNNNNNNNGG	6 cut(s)	73, 920, 1394, 1922, 2256, 2719
BseMI	GCAATG	2 cut(s)	1010, 2643
BseMII	CTCAG	5 cut(s)	1113, 1286, 1326, 1668, 2412
BseRI	GAGGAG	5 cut(s)	171, 204, 1304, 2819, 2975
BseSI	GKGCMC	1 cut(s)	914
BseXI	GCAGC	3 cut(s)	427, 1639, 3082
BseYI	CCCAGC	3 cut(s)	563, 572, 765
BsgI	GTGCAG	3 cut(s)	1455, 1671, 2097
BshFI	GGCC	9 cut(s)	357, 377, 436, 588, 1069, 1195, 1571, 2467, 2801
BshNI	GGYRCC	2 cut(s)	909, 1231
BshVI	ATCGAT	2 cut(s)	1299, 2518
BsiHKAI	GWGCWC	1 cut(s)	462
BsiHKCI	CYCGRG	1 cut(s)	1235
BsiSI	CCGG	2 cut(s)	1356, 1538
BslFI	GGGAC	5 cut(s)	1876, 2272, 2599, 2838, 3029
BslI	CCNNNNNNNGG	6 cut(s)	73, 920, 1394, 1922, 2256, 2719
BsmAI	GTCTC	7 cut(s)	82, 704, 1158, 1787, 1937, 2422, 2942
BsmFI	GGGAC	5 cut(s)	1876, 2272, 2599, 2838, 3029
BsnI	GGCC	9 cut(s)	357, 377, 436, 588, 1069, 1195, 1571, 2467, 2801
Bso31I	GGTCTC	2 cut(s)	82, 2942
BsoBI	CYCGRG	1 cut(s)	1235
Bsp119I	TTCGAA	1 cut(s)	499
Bsp1286I	GDGCHC	2 cut(s)	462, 914
Bsp1407I	TGTACA	1 cut(s)	312
Bsp143I	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
BspANI	GGCC	9 cut(s)	357, 377, 436, 588, 1069, 1195, 1571, 2467, 2801
BspCNI	CTCAG	5 cut(s)	1114, 1285, 1327, 1669, 2413
BspDI	ATCGAT	2 cut(s)	1299, 2518
BspHI	TCATGA	1 cut(s)	2304
BspPI	GGATC	5 cut(s)	807, 857, 2861, 2874, 2929
BspT104I	TTCGAA	1 cut(s)	499
BspT107I	GGYRCC	2 cut(s)	909, 1231
BspTNI	GGTCTC	2 cut(s)	82, 2942
BsrDI	GCAATG	2 cut(s)	1010, 2643
BsrGI	TGTACA	1 cut(s)	312
BssECI	CCNNGG	8 cut(s)	378, 423, 609, 1356, 2182, 2802, 2925, 3046
BssMI	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
BssSI	CACGAG	1 cut(s)	1482
Bst2BI	CACGAG	1 cut(s)	1482
Bst2UI	CCWGG	7 cut(s)	1192, 2183, 2488, 2926, 2939, 3048, 3071
Bst4CI	ACNGT	7 cut(s)	928, 1285, 1591, 1798, 2590, 2784, 3077
Bst6I	CTCTTC	4 cut(s)	145, 425, 936, 2940
BstAPI	GCANNNNNTGC	2 cut(s)	1009, 2084
BstAUI	TGTACA	1 cut(s)	312
BstBI	TTCGAA	1 cut(s)	499
BstC8I	GCNNGC	5 cut(s)	34, 420, 638, 690, 2362
BstDEI	CTNAG	8 cut(s)	1122, 1142, 1272, 1335, 1677, 1908, 2421, 2471
BstDSI	CCRYGG	2 cut(s)	423, 609
BstEII	GGTNACC	1 cut(s)	2653
BstH2I	RGCGCY	2 cut(s)	67, 2145
BstHHI	GCGC	2 cut(s)	66, 2144
BstKTI	GATC	9 cut(s)	754, 802, 852, 1020, 1036, 1303, 2869, 2913, 2937
BstMAI	GTCTC	7 cut(s)	82, 704, 1158, 1787, 1937, 2422, 2942
BstMBI	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
BstNI	CCWGG	7 cut(s)	1192, 2183, 2488, 2926, 2939, 3048, 3071
BstNSI	RCATGY	5 cut(s)	36, 553, 584, 640, 2087
BstPAI	GACNNNNGTC	1 cut(s)	2349
BstPI	GGTNACC	1 cut(s)	2653
BstSCI	CCNGG	8 cut(s)	1190, 1355, 2181, 2486, 2924, 2937, 3046, 3069
BstSFI	CTRYAG	1 cut(s)	37
BstSLI	GKGCMC	1 cut(s)	914
BstV1I	GCAGC	3 cut(s)	427, 1639, 3082
BstV2I	GAAGAC	3 cut(s)	734, 1031, 1354
BstX2I	RGATCY	2 cut(s)	849, 2866
BstXI	CCANNNNNNTGG	6 cut(s)	596, 669, 906, 1524, 2839, 3010
BstYI	RGATCY	2 cut(s)	849, 2866
Bsu15I	ATCGAT	2 cut(s)	1299, 2518
BsuI	GTATCC	2 cut(s)	570, 2158
BsuRI	GGCC	9 cut(s)	357, 377, 436, 588, 1069, 1195, 1571, 2467, 2801
BsuTUI	ATCGAT	2 cut(s)	1299, 2518
BtgI	CCRYGG	2 cut(s)	423, 609
BtgZI	GCGATG	1 cut(s)	2714
BtsI	GCAGTG	4 cut(s)	476, 1020, 1153, 2271
BtsIMutI	CAGTG	9 cut(s)	476, 1020, 1153, 1372, 1561, 1613, 1857, 2271, 2685
Cac8I	GCNNGC	5 cut(s)	34, 420, 638, 690, 2362
CciI	TCATGA	1 cut(s)	2304
CfoI	GCGC	2 cut(s)	66, 2144
Cfr13I	GGNCC	6 cut(s)	435, 840, 1912, 1999, 2465, 3073
ClaI	ATCGAT	2 cut(s)	1299, 2518
DdeI	CTNAG	8 cut(s)	1122, 1142, 1272, 1335, 1677, 1908, 2421, 2471
DpnI	GATC	9 cut(s)	753, 801, 851, 1019, 1035, 1302, 2868, 2912, 2936
DpnII	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
EaeI	YGGCCR	3 cut(s)	375, 586, 2799
Eam1104I	CTCTTC	4 cut(s)	145, 425, 936, 2940
EarI	CTCTTC	4 cut(s)	145, 425, 936, 2940
EciI	GGCGGA	3 cut(s)	413, 2447, 2879
Ecl136II	GAGCTC	1 cut(s)	460
Eco147I	AGGCCT	2 cut(s)	1195, 1571
Eco24I	GRGCYC	1 cut(s)	462
Eco31I	GGTCTC	2 cut(s)	82, 2942
Eco47I	GGWCC	4 cut(s)	840, 1912, 1999, 3073
Eco53kI	GAGCTC	1 cut(s)	460
Eco57I	CTGAAG	6 cut(s)	138, 326, 842, 912, 1325, 1348
Eco88I	CYCGRG	1 cut(s)	1235
Eco91I	GGTNACC	1 cut(s)	2653
EcoICRI	GAGCTC	1 cut(s)	460
EcoO109I	RGGNCCY	2 cut(s)	435, 1912
EcoO65I	GGTNACC	1 cut(s)	2653
EcoRI	GAATTC	1 cut(s)	2708
EcoRII	CCWGG	7 cut(s)	1190, 2181, 2486, 2924, 2937, 3046, 3069
EcoT22I	ATGCAT	2 cut(s)	679, 2645
EcoT38I	GRGCYC	1 cut(s)	462
FalI	AAGNNNNNCTT	2 cut(s)	1694, 1726
FaqI	GGGAC	5 cut(s)	1876, 2272, 2599, 2838, 3029
FauNDI	CATATG	4 cut(s)	790, 1027, 2796, 2845
Fnu4HI	GCNGC	8 cut(s)	334, 355, 396, 416, 1653, 2721, 3093, 3096
FriOI	GRGCYC	1 cut(s)	462
Fsp4HI	GCNGC	8 cut(s)	334, 355, 396, 416, 1653, 2721, 3093, 3096
FspBI	CTAG	7 cut(s)	351, 624, 1043, 1641, 1935, 2543, 2978
GlaI	GCGC	2 cut(s)	65, 2143
GluI	GCNGC	8 cut(s)	334, 355, 396, 416, 1653, 2721, 3093, 3096
GsaI	CCCAGC	3 cut(s)	567, 576, 769
HaeII	RGCGCY	2 cut(s)	67, 2145
HaeIII	GGCC	9 cut(s)	357, 377, 436, 588, 1069, 1195, 1571, 2467, 2801
HapII	CCGG	2 cut(s)	1356, 1538
HhaI	GCGC	2 cut(s)	66, 2144
Hin6I	GCGC	2 cut(s)	64, 2142
HinP1I	GCGC	2 cut(s)	64, 2142
HincII	GTYRAC	1 cut(s)	1254
HindII	GTYRAC	1 cut(s)	1254
HpaII	CCGG	2 cut(s)	1356, 1538
HphI	GGTGA	9 cut(s)	259, 439, 958, 1592, 1717, 1766, 1802, 2547, 2772
Hpy166II	GTNNAC	4 cut(s)	1254, 1708, 1757, 2555
Hpy8I	GTNNAC	4 cut(s)	1254, 1708, 1757, 2555
Hpy99I	CGWCG	1 cut(s)	223
HpyAV	CCTTC	6 cut(s)	1109, 1339, 1577, 2501, 2854, 2875
HpyCH4III	ACNGT	7 cut(s)	928, 1285, 1591, 1798, 2590, 2784, 3077
HpyCH4IV	ACGT	6 cut(s)	93, 494, 1256, 1744, 2612, 3084
HpyF3I	CTNAG	8 cut(s)	1122, 1142, 1272, 1335, 1677, 1908, 2421, 2471
HpySE526I	ACGT	6 cut(s)	93, 494, 1256, 1744, 2612, 3084
HspAI	GCGC	2 cut(s)	64, 2142
Kzo9I	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
LmnI	GCTCC	8 cut(s)	329, 400, 569, 694, 767, 1211, 2617, 2806
Lsp1109I	GCAGC	3 cut(s)	427, 1639, 3082
LweI	GCATC	8 cut(s)	152, 664, 999, 1249, 1508, 1524, 1944, 2956
MaeI	CTAG	7 cut(s)	351, 624, 1043, 1641, 1935, 2543, 2978
MaeII	ACGT	6 cut(s)	93, 494, 1256, 1744, 2612, 3084
MaeIII	GTNAC	7 cut(s)	41, 576, 644, 654, 1319, 2593, 2653
MalI	GATC	9 cut(s)	753, 801, 851, 1019, 1035, 1302, 2868, 2912, 2936
MboI	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
MfeI	CAATTG	1 cut(s)	904
MflI	RGATCY	2 cut(s)	849, 2866
MhlI	GDGCHC	2 cut(s)	462, 914
MlsI	TGGCCA	1 cut(s)	588
MluNI	TGGCCA	1 cut(s)	588
MlyI	GAGTC	2 cut(s)	2219, 2246
MmeI	TCCRAC	3 cut(s)	226, 2595, 2998
Mox20I	TGGCCA	1 cut(s)	588
Mph1103I	ATGCAT	2 cut(s)	679, 2645
MroXI	GAANNNNTTC	2 cut(s)	1404, 1761
MscI	TGGCCA	1 cut(s)	588
MseI	TTAA	8 cut(s)	771, 1392, 1629, 1713, 1718, 1902, 2715, 3027
Msp20I	TGGCCA	1 cut(s)	588
MspA1I	CMGCKG	4 cut(s)	74, 567, 692, 3095
MspI	CCGG	2 cut(s)	1356, 1538
MspR9I	CCNGG	8 cut(s)	1192, 1357, 2183, 2488, 2926, 2939, 3048, 3071
MunI	CAATTG	1 cut(s)	904
MvaI	CCWGG	7 cut(s)	1192, 2183, 2488, 2926, 2939, 3048, 3071
NciI	CCSGG	1 cut(s)	1357
NdeI	CATATG	4 cut(s)	790, 1027, 2796, 2845
NdeII	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
NmeAIII	GCCGAG	3 cut(s)	403, 1717, 2827
NmuCI	GTSAC	2 cut(s)	1319, 2593
NsiI	ATGCAT	2 cut(s)	679, 2645
NspI	RCATGY	5 cut(s)	36, 553, 584, 640, 2087
NspV	TTCGAA	1 cut(s)	499
PaeI	GCATGC	2 cut(s)	36, 640
PagI	TCATGA	1 cut(s)	2304
PceI	AGGCCT	2 cut(s)	1195, 1571
PciI	ACATGT	2 cut(s)	549, 580
PcsI	WCGNNNNNNNCGW	2 cut(s)	496, 1233
PdmI	GAANNNNTTC	2 cut(s)	1404, 1761
PfeI	GAWTC	8 cut(s)	244, 383, 783, 2318, 2435, 2477, 2885, 3055
PflFI	GACNNNGTC	1 cut(s)	1427
PfoI	TCCNGGA	1 cut(s)	3069
PkrI	GCNGC	8 cut(s)	335, 356, 397, 417, 1654, 2722, 3094, 3097
PleI	GAGTC	2 cut(s)	2219, 2246
PpsI	GAGTC	2 cut(s)	2219, 2246
PpuMI	RGGWCCY	1 cut(s)	1912
PscI	ACATGT	2 cut(s)	549, 580
PshAI	GACNNNNGTC	1 cut(s)	2349
PshBI	ATTAAT	1 cut(s)	1629
PsiI	TTATAA	3 cut(s)	986, 1595, 2727
Psp124BI	GAGCTC	1 cut(s)	462
Psp1406I	AACGTT	2 cut(s)	1256, 1744
Psp5II	RGGWCCY	1 cut(s)	1912
Psp6I	CCWGG	7 cut(s)	1190, 2181, 2486, 2924, 2937, 3046, 3069
PspEI	GGTNACC	1 cut(s)	2653
PspFI	CCCAGC	3 cut(s)	563, 572, 765
PspGI	CCWGG	7 cut(s)	1190, 2181, 2486, 2924, 2937, 3046, 3069
PspPI	GGNCC	6 cut(s)	435, 840, 1912, 1999, 2465, 3073
PspPPI	RGGWCCY	1 cut(s)	1912
PsuI	RGATCY	2 cut(s)	849, 2866
PsyI	GACNNNGTC	1 cut(s)	1427
PvuII	CAGCTG	1 cut(s)	3095
SacI	GAGCTC	1 cut(s)	462
SaqAI	TTAA	8 cut(s)	771, 1392, 1629, 1713, 1718, 1902, 2715, 3027
SatI	GCNGC	8 cut(s)	334, 355, 396, 416, 1653, 2721, 3093, 3096
Sau3AI	GATC	9 cut(s)	751, 799, 849, 1017, 1033, 1300, 2866, 2910, 2934
Sau96I	GGNCC	6 cut(s)	435, 840, 1912, 1999, 2465, 3073
SchI	GAGTC	2 cut(s)	2219, 2246
ScrFI	CCNGG	8 cut(s)	1192, 1357, 2183, 2488, 2926, 2939, 3048, 3071
SduI	GDGCHC	2 cut(s)	462, 914
SfaNI	GCATC	8 cut(s)	152, 664, 999, 1249, 1508, 1524, 1944, 2956
SfcI	CTRYAG	1 cut(s)	37
SfuI	TTCGAA	1 cut(s)	499
SinI	GGWCC	4 cut(s)	840, 1912, 1999, 3073
SmlI	CTYRAG	3 cut(s)	1013, 1309, 1325
SmoI	CTYRAG	3 cut(s)	1013, 1309, 1325
SphI	GCATGC	2 cut(s)	36, 640
SseBI	AGGCCT	2 cut(s)	1195, 1571
SspI	AATATT	1 cut(s)	830
SspMI	CTAG	7 cut(s)	351, 624, 1043, 1641, 1935, 2543, 2978
SstI	GAGCTC	1 cut(s)	462
StuI	AGGCCT	2 cut(s)	1195, 1571
StyD4I	CCNGG	8 cut(s)	1190, 1355, 2181, 2486, 2924, 2937, 3046, 3069
TaaI	ACNGT	7 cut(s)	928, 1285, 1591, 1798, 2590, 2784, 3077
TaiI	ACGT	6 cut(s)	96, 497, 1259, 1747, 2615, 3087
TaqI	TCGA	8 cut(s)	160, 242, 386, 456, 499, 1299, 2518, 3023
TatI	WGTACW	3 cut(s)	312, 1293, 2326
TauI	GCSGC	4 cut(s)	336, 357, 398, 2723
TfiI	GAWTC	8 cut(s)	244, 383, 783, 2318, 2435, 2477, 2885, 3055
Tru1I	TTAA	8 cut(s)	771, 1392, 1629, 1713, 1718, 1902, 2715, 3027
Tru9I	TTAA	8 cut(s)	771, 1392, 1629, 1713, 1718, 1902, 2715, 3027
TscAI	CASTG	9 cut(s)	476, 1027, 1153, 1372, 1561, 1620, 1864, 2271, 2692
TseFI	GTSAC	2 cut(s)	1319, 2593
TseI	GCWGC	4 cut(s)	415, 1652, 3092, 3095
Tsp45I	GTSAC	2 cut(s)	1319, 2593
TspDTI	ATGAA	9 cut(s)	17, 525, 954, 1013, 2084, 2321, 2449, 2466, 3006
TspGWI	ACGGA	3 cut(s)	207, 237, 626
TspRI	CASTG	9 cut(s)	476, 1027, 1153, 1372, 1561, 1620, 1864, 2271, 2692
Tth111I	GACNNNGTC	1 cut(s)	1427
VpaK11BI	GGWCC	4 cut(s)	840, 1912, 1999, 3073
VspI	ATTAAT	1 cut(s)	1629
XbaI	TCTAGA	1 cut(s)	1042
XceI	RCATGY	5 cut(s)	36, 553, 584, 640, 2087
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1002
XmnI	GAANNNNTTC	2 cut(s)	1404, 1761
XspI	CTAG	7 cut(s)	351, 624, 1043, 1641, 1935, 2543, 2978
Zsp2I	ATGCAT	2 cut(s)	679, 2645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.