pycom111g03910
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0011709
Physical Location & Seq
Forward (+)
2373952 .. 2375703
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGAAGAAAATGCTAGATTAAAAATGGCGTTAAATCATATGGAGAAGGATTACCATTCTCTCCAGCTGCGGTTTTTTGACATCCTCAAGCAACAGGCTTCCAAAAAAGCTGTAAATGTCGCGGGTCATCGAATAATCGAAGAACCGGAGGAGCTCTTGTCTCTTTGTCTTGGAAGAAGTCCAAGGGAACCTAAAAATGATCGTGAAACTACTGCCGGTAGCAACTTCACAAAACTTGCACAAGTTGATGATGGTGAAGAGAACTTGAAGGCCAATCTTACTCTTGGATTAGGTTCTGATGAGACTAGTTTGGAAGAACCTAATAAGAATCATGCAGAAGCTGGAGGTGGAGAAGCGTCGTCGCCGCCATCGAGTAAAACGCCGAAGACGATGAGGAATGAAGACGGTGAACCTCCACCGCAGGTCAATGCAAAACGAGCCAGGGTTTCTGTGAGAACAAGATGTGATGCCCCAACGATGAATGATGGATGCCAATGGAGAAAATATGGACAGAAGACTGCAAAAGGAAATCCATGCCCGCGAGCATACTATCGCTGCACAGTTGCACCAGCGTGCCCTGTGAGAAAGCAGGTGCAAAGATGTTATGAGGATATGTCCATCTTAATCACCACCTATGAAGGAACTCACAACCACCCACTTCCATTCACAGCCACTTGCATGGCTTCCACCACCTCTGCCGCAGCTTCCATGCTTTTGTCCGGCTCTTCCACATCTCACCCGGGCTTCGATTCCACAGCCACCCTAATCAATGGATCAGAATTTCGTGCCTTCGATAACTCAAGAACACAACTCTACATGCCCAAATCTGCCCACCCTTTGCTCCCCACAATCACTCTCGACCTCACTGCCTCTACATCCTCTTCTAGCACATTTCCTTCAAGTCTAAGCTTTTGCTCCCCGTCAGATTCCAACAATTTGCCCATAAGTTGGGGCAATGAATACCTTAGATATGGCTTGCTACTGTCCGACAAAACTCAAGTTGGATCCTACAATCTTGGAAAGGCATCCCAAGAGTATTTCCATCAAACTTATGCAGAAAAGATCCATCAAGCTTCCTTCCAGGTGTCTTTGACAGAATCACTAACCAACGCAATCACGTCCGACCCGAATTTCAAGTCGGTAATTGCAGTAGCACTTTCATCTATGGTCGGAGGTGGTGGTGACGCCACCCATAGGAACCAAGGCGAAGGTGGCGAAAGAATGGGACAACAGTTGAAGTGGGGTGAAGCTGCTCATCAGTTTACTTCTCGAAAGTGA

Protein Analysis

426

Amino Acids

46.41

Weight (kDa)

7.18

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 161 - 219 4.2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15130 AT5G15130
fragaria_vesca FvH4_6g49140 FvH4_6g49140
malus_domestica MD09G1048200.v1.1 MD09G1048300.v1.1 MD17G1048400.v1.1
prunus_persica Prupe.3G270800_v2.0.a1
pyrus_communis pycom111g03910
rosa_chinensis RchiOBHm_Chr2g0169011
rosa_laevigata RLG00000021823
rosa_multiflora Rmu_ssc0000393.1_g000021
rosa_roxburghii Rroxscaffold_2G00082540
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607700 Rh2BG620200 Rh2CG589200 Rh2DG631000
rosa_wichuraiana Rw2G050390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 2 cut(s) 412, 580
AccB7I CCANNNNNTGG 1 cut(s) 948
AccII CGCG 2 cut(s) 122, 541
AciI CCGC 6 cut(s) 70, 122, 365, 419, 539, 699
AclWI GGATC 4 cut(s) 781, 999, 1012, 1057
AcsI RAATTY 2 cut(s) 779, 1129
AcyI GRCGYC 1 cut(s) 1185
AfiI CCNNNNNNNGG 2 cut(s) 948, 1194
AgsI TTSAA 4 cut(s) 268, 900, 1135, 1237
AhlI ACTAGT 1 cut(s) 305
AjiI CACGTC 1 cut(s) 1119
AjnI CCWGG 2 cut(s) 440, 1080
AleI CACNNNNGTG 1 cut(s) 571
AluBI AGCT 8 cut(s) 67, 110, 154, 341, 704, 909, 1073, 1250
AluI AGCT 8 cut(s) 67, 110, 154, 341, 704, 909, 1073, 1250
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 2 cut(s) 165, 296
AlwI GGATC 4 cut(s) 781, 999, 1012, 1057
AlwNI CAGNNNCTG 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 4 cut(s) 67, 555, 701, 1250
ApoI RAATTY 2 cut(s) 779, 1129
ArsI GACNNNNNNTTYG 2 cut(s) 1121, 1153
AsuC2I CCSGG 2 cut(s) 740, 741
AsuHPI GGTGA 6 cut(s) 266, 419, 619, 728, 1193, 1256
AvaI CYCGRG 1 cut(s) 739
BaeGI GKGCMC 1 cut(s) 578
BamHI GGATCC 1 cut(s) 1004
BanII GRGCYC 1 cut(s) 156
BbsI GAAGAC 3 cut(s) 392, 408, 521
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 4 cut(s) 54, 542, 713, 1237
BccI CCATC 6 cut(s) 245, 376, 479, 626, 1050, 1074
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 2 cut(s) 442, 1082
BcnI CCSGG 2 cut(s) 740, 741
BcoDI GTCTC 2 cut(s) 165, 296
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 3 cut(s) 15, 306, 885
BfuAI ACCTGC 2 cut(s) 412, 580
BisI GCNGC 6 cut(s) 68, 365, 556, 699, 702, 1251
BlsI GCNGC 6 cut(s) 69, 366, 557, 700, 703, 1252
Bme1390I CCNGG 4 cut(s) 442, 740, 741, 1082
BmeT110I CYCGRG 1 cut(s) 739
BmgBI CACGTC 1 cut(s) 1119
BmiI GGNNCC 3 cut(s) 189, 1006, 1199
BmrFI CCNGG 4 cut(s) 442, 740, 741, 1082
BmsI GCATC 3 cut(s) 457, 479, 1034
BpiI GAAGAC 3 cut(s) 392, 408, 521
BpmI CTGGAG 2 cut(s) 47, 363
BpuEI CTTGAG 3 cut(s) 71, 784, 981
BpuMI CCSGG 2 cut(s) 740, 741
BsaHI GRCGYC 1 cut(s) 1185
BsaJI CCNNGG 4 cut(s) 182, 441, 739, 1201
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 2 cut(s) 948, 1194
Bse118I RCCGGY 1 cut(s) 215
Bse3DI GCAATG 1 cut(s) 961
BseBI CCWGG 2 cut(s) 442, 1082
BseDI CCNNGG 4 cut(s) 182, 441, 739, 1201
BseGI GGATG 4 cut(s) 81, 494, 875, 1025
BseLI CCNNNNNNNGG 2 cut(s) 948, 1194
BseMI GCAATG 1 cut(s) 961
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 4 cut(s) 54, 542, 713, 1237
BsgI GTGCAG 1 cut(s) 541
Bsh1236I CGCG 2 cut(s) 122, 541
BshFI GGCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 4 cut(s) 146, 216, 720, 740
BslFI GGGAC 1 cut(s) 1239
BslI CCNNNNNNNGG 2 cut(s) 948, 1194
BsmAI GTCTC 2 cut(s) 165, 296
BsmFI GGGAC 1 cut(s) 1239
BsnI GGCC 1 cut(s) 272
BsoBI CYCGRG 1 cut(s) 739
Bsp1286I GDGCHC 2 cut(s) 156, 578
Bsp143I GATC 4 cut(s) 199, 773, 1004, 1062
BspACI CCGC 6 cut(s) 70, 122, 365, 419, 539, 699
BspANI GGCC 1 cut(s) 272
BspFNI CGCG 2 cut(s) 122, 541
BspLI GGNNCC 3 cut(s) 189, 1006, 1199
BspMI ACCTGC 2 cut(s) 412, 580
BspPI GGATC 4 cut(s) 781, 999, 1012, 1057
BspQI GCTCTTC 1 cut(s) 730
BsrDI GCAATG 1 cut(s) 961
BsrFI RCCGGY 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 4 cut(s) 182, 441, 739, 1201
BssMI GATC 4 cut(s) 199, 773, 1004, 1062
BssNI GRCGYC 1 cut(s) 1185
BssT1I CCWWGG 2 cut(s) 182, 1201
Bst2UI CCWGG 2 cut(s) 442, 1082
Bst4CI ACNGT 4 cut(s) 407, 562, 984, 1233
Bst6I CTCTTC 3 cut(s) 252, 730, 886
BstACI GRCGYC 1 cut(s) 1185
BstC8I GCNNGC 4 cut(s) 539, 543, 574, 977
BstDEI CTNAG 2 cut(s) 905, 965
BstF5I GGATG 4 cut(s) 81, 494, 875, 1025
BstFNI CGCG 2 cut(s) 122, 541
BstKTI GATC 4 cut(s) 202, 776, 1007, 1065
BstMAI GTCTC 2 cut(s) 165, 296
BstMBI GATC 4 cut(s) 199, 773, 1004, 1062
BstMWI GCNNNNNNNGC 2 cut(s) 437, 1212
BstNI CCWGG 2 cut(s) 442, 1082
BstNSI RCATGY 1 cut(s) 820
BstSCI CCNGG 4 cut(s) 440, 738, 739, 1080
BstSLI GKGCMC 1 cut(s) 578
BstUI CGCG 2 cut(s) 122, 541
BstV1I GCAGC 4 cut(s) 54, 542, 713, 1237
BstV2I GAAGAC 3 cut(s) 392, 408, 521
BstX2I RGATCY 2 cut(s) 1004, 1062
BstXI CCANNNNNNTGG 1 cut(s) 679
BstYI RGATCY 2 cut(s) 1004, 1062
BsuRI GGCC 1 cut(s) 272
BtrI CACGTC 1 cut(s) 1119
BtsCI GGATG 4 cut(s) 81, 494, 875, 1025
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 1 cut(s) 864
BveI ACCTGC 2 cut(s) 412, 580
Cac8I GCNNGC 4 cut(s) 539, 543, 574, 977
CaiI CAGNNNCTG 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 215
Cfr9I CCCGGG 1 cut(s) 739
CseI GACGC 2 cut(s) 345, 1193
CviAII CATG 5 cut(s) 332, 534, 679, 709, 817
DdeI CTNAG 2 cut(s) 905, 965
DpnI GATC 4 cut(s) 201, 775, 1006, 1064
DpnII GATC 4 cut(s) 199, 773, 1004, 1062
Eam1104I CTCTTC 3 cut(s) 252, 730, 886
EarI CTCTTC 3 cut(s) 252, 730, 886
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 182, 1201
Eco24I GRGCYC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 739
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 440, 1080
EcoT14I CCWWGG 2 cut(s) 182, 1201
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 2 cut(s) 182, 1201
FaeI CATG 5 cut(s) 335, 537, 682, 712, 820
FaqI GGGAC 1 cut(s) 1239
FatI CATG 5 cut(s) 331, 533, 678, 708, 816
FauI CCCGC 2 cut(s) 115, 546
FauNDI CATATG 1 cut(s) 39
Fnu4HI GCNGC 6 cut(s) 68, 365, 556, 699, 702, 1251
FokI GGATG 4 cut(s) 68, 501, 862, 1012
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 6 cut(s) 68, 365, 556, 699, 702, 1251
FspBI CTAG 3 cut(s) 15, 306, 885
GluI GCNGC 6 cut(s) 68, 365, 556, 699, 702, 1251
GsuI CTGGAG 2 cut(s) 47, 363
HaeIII GGCC 1 cut(s) 272
HapII CCGG 4 cut(s) 146, 216, 720, 740
HgaI GACGC 2 cut(s) 345, 1193
Hin1I GRCGYC 1 cut(s) 1185
Hin1II CATG 5 cut(s) 335, 537, 682, 712, 820
HindIII AAGCTT 2 cut(s) 907, 1071
HinfI GANTC 4 cut(s) 328, 749, 926, 1097
HpaII CCGG 4 cut(s) 146, 216, 720, 740
HphI GGTGA 6 cut(s) 266, 419, 619, 728, 1193, 1256
Hpy166II GTNNAC 2 cut(s) 410, 1263
Hpy188I TCNGA 6 cut(s) 298, 778, 925, 988, 1123, 1172
Hpy188III TCNNGA 4 cut(s) 203, 801, 857, 1269
Hpy8I GTNNAC 2 cut(s) 410, 1263
Hpy99I CGWCG 2 cut(s) 361, 364
HpyAV CCTTC 7 cut(s) 40, 262, 632, 799, 906, 1087, 1202
HpyCH4III ACNGT 4 cut(s) 407, 562, 984, 1233
HpyCH4IV ACGT 1 cut(s) 1118
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 1212
HpyF3I CTNAG 2 cut(s) 905, 965
HpySE526I ACGT 1 cut(s) 1118
Hsp92I GRCGYC 1 cut(s) 1185
Hsp92II CATG 5 cut(s) 335, 537, 682, 712, 820
Kzo9I GATC 4 cut(s) 199, 773, 1004, 1062
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 3 cut(s) 151, 846, 920
Lsp1109I GCAGC 4 cut(s) 54, 542, 713, 1237
LweI GCATC 3 cut(s) 457, 479, 1034
MaeI CTAG 3 cut(s) 15, 306, 885
MaeII ACGT 1 cut(s) 1118
MaeIII GTNAC 1 cut(s) 1181
MalI GATC 4 cut(s) 201, 775, 1006, 1064
MboI GATC 4 cut(s) 199, 773, 1004, 1062
MflI RGATCY 2 cut(s) 1004, 1062
MhlI GDGCHC 2 cut(s) 156, 578
MluCI AATT 4 cut(s) 779, 934, 1129, 1143
MmeI TCCRAC 5 cut(s) 954, 982, 1011, 1146, 1150
MseI TTAA 3 cut(s) 20, 32, 623
MslI CAYNNNNRTG 2 cut(s) 571, 677
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 4 cut(s) 146, 216, 720, 740
MspR9I CCNGG 4 cut(s) 442, 740, 741, 1082
MvaI CCWGG 2 cut(s) 442, 1082
MvnI CGCG 2 cut(s) 122, 541
MwoI GCNNNNNNNGC 2 cut(s) 437, 1212
NciI CCSGG 2 cut(s) 740, 741
NdeI CATATG 1 cut(s) 39
NdeII GATC 4 cut(s) 199, 773, 1004, 1062
NlaIII CATG 5 cut(s) 335, 537, 682, 712, 820
NlaIV GGNNCC 3 cut(s) 189, 1006, 1199
NmuCI GTSAC 1 cut(s) 1181
NspI RCATGY 1 cut(s) 820
OliI CACNNNNGTG 1 cut(s) 571
PaqCI CACCTGC 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 730
PcsI WCGNNNNNNNCGW 3 cut(s) 368, 386, 1124
PfeI GAWTC 4 cut(s) 328, 749, 926, 1097
PflMI CCANNNNNTGG 1 cut(s) 948
PkrI GCNGC 6 cut(s) 69, 366, 557, 700, 703, 1252
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 440, 1080
PspGI CCWGG 2 cut(s) 440, 1080
PspN4I GGNNCC 3 cut(s) 189, 1006, 1199
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 2 cut(s) 1004, 1062
PvuII CAGCTG 1 cut(s) 67
RseI CAYNNNNRTG 2 cut(s) 571, 677
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 730
SaqAI TTAA 3 cut(s) 20, 32, 623
SatI GCNGC 6 cut(s) 68, 365, 556, 699, 702, 1251
Sau3AI GATC 4 cut(s) 199, 773, 1004, 1062
ScrFI CCNGG 4 cut(s) 442, 740, 741, 1082
SduI GDGCHC 2 cut(s) 156, 578
SfaNI GCATC 3 cut(s) 457, 479, 1034
SmaI CCCGGG 1 cut(s) 741
SmiMI CAYNNNNRTG 2 cut(s) 571, 677
SmlI CTYRAG 3 cut(s) 86, 799, 996
SmoI CTYRAG 3 cut(s) 86, 799, 996
SpeI ACTAGT 1 cut(s) 305
Sse9I AATT 4 cut(s) 779, 934, 1129, 1143
SsiI CCGC 6 cut(s) 70, 122, 365, 419, 539, 699
SspMI CTAG 3 cut(s) 15, 306, 885
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 4 cut(s) 440, 738, 739, 1080
StyI CCWWGG 2 cut(s) 182, 1201
TaaI ACNGT 4 cut(s) 407, 562, 984, 1233
TaiI ACGT 1 cut(s) 1121
TaqI TCGA 7 cut(s) 130, 138, 371, 747, 792, 858, 1270
TasI AATT 4 cut(s) 779, 934, 1129, 1143
TauI GCSGC 2 cut(s) 367, 701
TfiI GAWTC 4 cut(s) 328, 749, 926, 1097
Tru1I TTAA 3 cut(s) 20, 32, 623
Tru9I TTAA 3 cut(s) 20, 32, 623
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 1 cut(s) 1181
TseI GCWGC 4 cut(s) 67, 555, 701, 1250
Tsp45I GTSAC 1 cut(s) 1181
TspDTI ATGAA 5 cut(s) 414, 494, 651, 972, 1149
TspMI CCCGGG 1 cut(s) 739
TspRI CASTG 1 cut(s) 871
Van91I CCANNNNNTGG 1 cut(s) 948
XapI RAATTY 2 cut(s) 779, 1129
XceI RCATGY 1 cut(s) 820
XcmI CCANNNNNNNNNTGG 1 cut(s) 1208
XmaI CCCGGG 1 cut(s) 739
XspI CTAG 3 cut(s) 15, 306, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.