pycom111g03920

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0018831
Physical Location & Seq
Reverse (-)
2379208 .. 2380392
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGGTTTTCTCAGAAGAGCTTCCAAAACCATGAAGCCCTCCAAGTACTTCTCTTTGCTGCAGAACAACTCGCTCTCTAGCATTTCCAGACGCAACCCACTTCGAAACGACTTCGATTACTGCCGTTTTGGGCTGAGCCATGCGTTTTGCTCCTACTCTCGACAAAACAGCAAGGAGAACGCCGTAGATTTGAGCCAGTATCCGACGGAGAGGATTCGGAACTTCTCGATAATCGCGCATGTCGATCACGGCAAGTCTACTCTTGCCGACAGGCTTCTGGAGCTCACTGGGACTATCAAGAGAGGCCATGGCCAGCCTCAGTACCTCGACAAATTGCAGGTGGAGAGAGAAAGGGGAATTACTGTTAAAGCGCAGACAGCTACCATGTTCTACAACTACAAGCAGAGTCTTAACAGTGCGAAAGACAACAATGGTGACCCAAAAGAACAAAGCTTTTTATTGAATCTGATTGACACGCCTGGTCATGTGGATTTTAGCTATGAAGTTTCAAGATCTCTAGCTGCTTGCCAAGGTGCCCTTTTGGTTGTTGATGCTGCACAGGGTGTTCAAGCACAAACTGTTGCAAACTTCTATCTTGCCTTTGAATCCAACCTGACAATAATACCCGTTATTAACAAGATTGACCAGCCAACTGCTGATCCTGATCGATTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

226

Amino Acids

25.38

Weight (kDa)

9.22

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 70 - 222 5.5e-49 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018831)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12911
pyrus_communis pycom111g03920
rosa_laevigata RLG00000020534 RLG00000023537 RLG00000028425 RLG00000035877
rosa_samantha Rh2AG244200 Rh2AG539500 Rh2DG253000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 328
AccB1I GGYRCC 1 cut(s) 533
AccI GTMKAC 1 cut(s) 257
AccII CGCG 1 cut(s) 236
AclWI GGATC 1 cut(s) 653
AcoI YGGCCR 1 cut(s) 310
AdeI CACNNNGTG 1 cut(s) 563
AfaI GTAC 2 cut(s) 47, 323
AgsI TTSAA 4 cut(s) 463, 510, 569, 605
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 6 cut(s) 20, 283, 380, 453, 498, 521
AluI AGCT 6 cut(s) 20, 283, 380, 453, 498, 521
Alw21I GWGCWC 1 cut(s) 285
AlwI GGATC 1 cut(s) 653
AoxI GGCC 2 cut(s) 304, 310
ApeKI GCWGC 3 cut(s) 58, 521, 554
Asp700I GAANNNNTTC 1 cut(s) 19
AspLEI GCGC 2 cut(s) 238, 373
AsuHPI GGTGA 1 cut(s) 446
AsuII TTCGAA 1 cut(s) 103
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 312
BanI GGYRCC 1 cut(s) 533
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 3 cut(s) 45, 508, 541
BceAI ACGGC 3 cut(s) 108, 167, 265
BciT130I CCWGG 1 cut(s) 480
BciVI GTATCC 1 cut(s) 210
BfaI CTAG 2 cut(s) 78, 518
BfmI CTRYAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 328
BfuI GTATCC 1 cut(s) 210
BglII AGATCT 1 cut(s) 512
BisI GCNGC 3 cut(s) 59, 522, 555
BlpI GCTNAGC 1 cut(s) 134
BlsI GCNGC 3 cut(s) 60, 523, 556
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 1 cut(s) 480
BmiI GGNNCC 1 cut(s) 535
BmrFI CCNGG 1 cut(s) 480
BmrI ACTGGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 299
Bpu1102I GCTNAGC 1 cut(s) 134
Bpu14I TTCGAA 1 cut(s) 103
Bsa29I ATCGAT 1 cut(s) 667
BsaBI GATNNNNATC 1 cut(s) 663
BsaJI CCNNGG 2 cut(s) 307, 529
Bse1I ACTGG 2 cut(s) 196, 292
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 667
BseDI CCNNGG 2 cut(s) 307, 529
BseJI GATNNNNATC 1 cut(s) 663
BseMII CTCAG 3 cut(s) 25, 125, 332
BseNI ACTGG 2 cut(s) 196, 292
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 3 cut(s) 45, 508, 541
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 306, 312
BshNI GGYRCC 1 cut(s) 533
BshVI ATCGAT 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 285
BslFI GGGAC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 2 cut(s) 306, 312
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 2 cut(s) 285, 538
Bsp143I GATC 4 cut(s) 244, 512, 658, 664
Bsp1720I GCTNAGC 1 cut(s) 134
Bsp19I CCATGG 1 cut(s) 307
BspANI GGCC 2 cut(s) 306, 312
BspCNI CTCAG 3 cut(s) 24, 126, 331
BspDI ATCGAT 1 cut(s) 667
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 1 cut(s) 535
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 1 cut(s) 653
BspQI GCTCTTC 1 cut(s) 10
BspT104I TTCGAA 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 533
BsrI ACTGG 2 cut(s) 196, 292
BssECI CCNNGG 2 cut(s) 307, 529
BssMI GATC 4 cut(s) 244, 512, 658, 664
BssT1I CCWWGG 2 cut(s) 307, 529
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 3 cut(s) 364, 416, 580
Bst6I CTCTTC 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 314, 526
BstDEI CTNAG 3 cut(s) 11, 134, 318
BstDSI CCRYGG 1 cut(s) 307
BstEII GGTNACC 1 cut(s) 434
BstFNI CGCG 1 cut(s) 236
BstHHI GCGC 2 cut(s) 238, 373
BstKTI GATC 4 cut(s) 247, 515, 661, 667
BstMBI GATC 4 cut(s) 244, 512, 658, 664
BstMWI GCNNNNNNNGC 2 cut(s) 280, 377
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 1 cut(s) 242
BstPI GGTNACC 1 cut(s) 434
BstSCI CCNGG 1 cut(s) 478
BstSFI CTRYAG 1 cut(s) 59
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 3 cut(s) 45, 508, 541
BstX2I RGATCY 1 cut(s) 512
BstYI RGATCY 1 cut(s) 512
Bsu15I ATCGAT 1 cut(s) 667
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 306, 312
BsuTUI ATCGAT 1 cut(s) 667
BtgI CCRYGG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 285, 421
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 314, 526
CfoI GCGC 2 cut(s) 238, 373
ClaI ATCGAT 1 cut(s) 667
CseI GACGC 1 cut(s) 99
Csp6I GTAC 2 cut(s) 46, 322
CviAII CATG 6 cut(s) 31, 140, 239, 308, 385, 485
CviQI GTAC 2 cut(s) 46, 322
DdeI CTNAG 3 cut(s) 11, 134, 318
DpnI GATC 4 cut(s) 246, 514, 660, 666
DpnII GATC 4 cut(s) 244, 512, 658, 664
DraI TTTAAA 1 cut(s) 673
DraIII CACNNNGTG 1 cut(s) 563
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 307, 529
Eco24I GRGCYC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 283
Eco91I GGTNACC 1 cut(s) 434
EcoICRI GAGCTC 1 cut(s) 283
EcoO65I GGTNACC 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 2 cut(s) 307, 529
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 2 cut(s) 307, 529
FaeI CATG 6 cut(s) 34, 143, 242, 311, 388, 488
FaiI YATR 7 cut(s) 32, 141, 240, 309, 386, 486, 501
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 1 cut(s) 304
FatI CATG 6 cut(s) 30, 139, 238, 307, 384, 484
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 3 cut(s) 59, 522, 555
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 3 cut(s) 59, 522, 555
FspBI CTAG 2 cut(s) 78, 518
GlaI GCGC 2 cut(s) 237, 372
GluI GCNGC 3 cut(s) 59, 522, 555
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 2 cut(s) 306, 312
HgaI GACGC 1 cut(s) 99
HhaI GCGC 2 cut(s) 238, 373
Hin1II CATG 6 cut(s) 34, 143, 242, 311, 388, 488
Hin6I GCGC 2 cut(s) 236, 371
HinP1I GCGC 2 cut(s) 236, 371
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 4 cut(s) 214, 406, 463, 605
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 4 cut(s) 14, 204, 219, 468
Hpy188III TCNNGA 7 cut(s) 87, 159, 226, 278, 298, 510, 662
Hpy8I GTNNAC 1 cut(s) 258
Hpy99I CGWCG 1 cut(s) 208
HpyCH4III ACNGT 3 cut(s) 364, 416, 580
HpyCH4V TGCA 4 cut(s) 61, 337, 557, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 377
HpyF3I CTNAG 3 cut(s) 11, 134, 318
Hsp92II CATG 6 cut(s) 34, 143, 242, 311, 388, 488
HspAI GCGC 2 cut(s) 236, 371
Kzo9I GATC 4 cut(s) 244, 512, 658, 664
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 2 cut(s) 155, 280
Lsp1109I GCAGC 3 cut(s) 45, 508, 541
LweI GCATC 1 cut(s) 541
MaeI CTAG 2 cut(s) 78, 518
MaeIII GTNAC 1 cut(s) 434
MalI GATC 4 cut(s) 246, 514, 660, 666
MboI GATC 4 cut(s) 244, 512, 658, 664
MboII GAAGA 1 cut(s) 27
MflI RGATCY 1 cut(s) 512
MhlI GDGCHC 2 cut(s) 285, 538
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 2 cut(s) 332, 357
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 2 cut(s) 227, 633
MnlI CCTC 5 cut(s) 49, 204, 296, 327, 335
Mox20I TGGCCA 1 cut(s) 312
MroXI GAANNNNTTC 1 cut(s) 19
MscI TGGCCA 1 cut(s) 312
MseI TTAA 4 cut(s) 366, 411, 633, 672
Msp20I TGGCCA 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 2 cut(s) 280, 377
NcoI CCATGG 1 cut(s) 307
NdeII GATC 4 cut(s) 244, 512, 658, 664
NlaIII CATG 6 cut(s) 34, 143, 242, 311, 388, 488
NlaIV GGNNCC 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 434
NspI RCATGY 1 cut(s) 242
NspV TTCGAA 1 cut(s) 103
PaqCI CACCTGC 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 214, 463, 605
PkrI GCNGC 3 cut(s) 60, 523, 556
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 478
PspEI GGTNACC 1 cut(s) 434
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 535
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 1 cut(s) 512
RsaI GTAC 2 cut(s) 47, 323
RsaNI GTAC 2 cut(s) 46, 322
SacI GAGCTC 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 4 cut(s) 366, 411, 633, 672
SatI GCNGC 3 cut(s) 59, 522, 555
Sau3AI GATC 4 cut(s) 244, 512, 658, 664
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 1 cut(s) 480
SduI GDGCHC 2 cut(s) 285, 538
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 1 cut(s) 59
SfuI TTCGAA 1 cut(s) 103
SmiI ATTTAAAT 1 cut(s) 673
Sse9I AATT 2 cut(s) 332, 357
SspMI CTAG 2 cut(s) 78, 518
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 478
StyI CCWWGG 2 cut(s) 307, 529
SwaI ATTTAAAT 1 cut(s) 673
TaaI ACNGT 3 cut(s) 364, 416, 580
TaqI TCGA 7 cut(s) 103, 114, 160, 227, 243, 327, 667
TasI AATT 2 cut(s) 332, 357
TatI WGTACW 1 cut(s) 45
TfiI GAWTC 3 cut(s) 214, 463, 605
Tru1I TTAA 4 cut(s) 366, 411, 633, 672
Tru9I TTAA 4 cut(s) 366, 411, 633, 672
TscAI CASTG 2 cut(s) 292, 421
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 3 cut(s) 58, 521, 554
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 2 cut(s) 47, 516
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 2 cut(s) 292, 421
XceI RCATGY 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 2 cut(s) 78, 518
ZrmI AGTACT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.