pycom111g04900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0019307
Physical Location & Seq
Reverse (-)
3008616 .. 3009273
658 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g04900.1

Sequence Viewer

Length: 588 bp
ATGGAAGAAAAACCTGAGAATCCCTCGTCCGACCATTGGTACCATTGGCCTGAAGTAATTAAGCCCCTCTTGTTAATCCTCTTCGTAGGCGGCTTGAGCATAATCTTTTACATCCTGGCGGCGCACGAACACTCGCTCAACCCCTTGGCCCCCGAGTTCCGCATCTACTCGGCCGCCGTGTCCCCGTGCCCACTCGCCCCGACCCCTCCTTCCGCCAGCCTCCAGGCAGATCTCTCCTACGCGGACGAGGAGACGAAGGACAAACTGATGCTCGCGACGACGCCGTTGCCTCTTTTGGCTTTAACCATCATGAACCAGACCACTTTCAATGTCACCTTCGCGACGGCGGGGGCCTTCGTCAGCGATGACGCCGCCAAGGAATTAATAATCTCCTCTAACTCCTCGTCAGCAGGTGATGGTTGTTATGGGTCGGCCAAATTGGGGGTGAAGCTGACCGGATATTTCAGAATTGATAAAGGTCTCTGGTTTTCGCGTTACTTCTGCGACCTTTCTCCCCCCAATTACAACGTCACCGATCAGTCAAGCCAATGTGAAATTTATAATACTGTTTATCGGTGTGTATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.44

Weight (kDa)

4.85

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 561
AarI CACCTGC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 401
Acc65I GGTACC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 39
AccII CGCG 4 cut(s) 242, 275, 341, 493
AciI CCGC 8 cut(s) 90, 119, 160, 174, 213, 242, 347, 372
AcoI YGGCCR 2 cut(s) 171, 432
AcsI RAATTY 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 72
AcyI GRCGYC 2 cut(s) 281, 369
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 36, 441
AgsI TTSAA 1 cut(s) 328
AjnI CCWGG 2 cut(s) 114, 222
AluBI AGCT 1 cut(s) 451
AluI AGCT 1 cut(s) 451
Alw26I GTCTC 2 cut(s) 245, 485
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 5 cut(s) 47, 147, 171, 351, 432
ApoI RAATTY 1 cut(s) 555
AseI ATTAAT 1 cut(s) 383
Asp718I GGTACC 1 cut(s) 39
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 2 cut(s) 148, 351
AsuHPI GGTGA 4 cut(s) 325, 425, 457, 523
AvaI CYCGRG 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 191
BanI GGYRCC 1 cut(s) 39
BccI CCATC 2 cut(s) 314, 410
BceAI ACGGC 3 cut(s) 161, 268, 360
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 2 cut(s) 116, 224
BcoDI GTCTC 2 cut(s) 245, 485
BfuAI ACCTGC 1 cut(s) 401
BglII AGATCT 1 cut(s) 229
BisI GCNGC 4 cut(s) 91, 120, 174, 372
BlsI GCNGC 4 cut(s) 92, 121, 175, 373
Bme1390I CCNGG 2 cut(s) 116, 224
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 2 cut(s) 148, 351
BmiI GGNNCC 3 cut(s) 41, 150, 352
BmrFI CCNGG 2 cut(s) 116, 224
BmsI GCATC 2 cut(s) 171, 258
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpmI CTGGAG 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 115
BsaHI GRCGYC 2 cut(s) 281, 369
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 2 cut(s) 144, 375
BsaWI WCCGGW 1 cut(s) 455
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 441
BseBI CCWGG 2 cut(s) 116, 224
BseDI CCNNGG 2 cut(s) 144, 375
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 36, 441
BseMII CTCAG 1 cut(s) 6
BseRI GAGGAG 3 cut(s) 263, 382, 391
BseSI GKGCMC 1 cut(s) 191
BseX3I CGGCCG 1 cut(s) 171
Bsh1236I CGCG 4 cut(s) 242, 275, 341, 493
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 5 cut(s) 49, 149, 173, 353, 434
BshNI GGYRCC 1 cut(s) 39
BsiEI CGRYCG 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 456
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 2 cut(s) 36, 441
BsmAI GTCTC 2 cut(s) 245, 485
BsmBI CGTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 5 cut(s) 49, 149, 173, 353, 434
Bso31I GGTCTC 1 cut(s) 485
BsoBI CYCGRG 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 2 cut(s) 229, 535
Bsp68I TCGCGA 2 cut(s) 275, 341
BspACI CCGC 8 cut(s) 90, 119, 160, 174, 213, 242, 347, 372
BspANI GGCC 5 cut(s) 49, 149, 173, 353, 434
BspCNI CTCAG 1 cut(s) 7
BspFNI CGCG 4 cut(s) 242, 275, 341, 493
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 3 cut(s) 41, 150, 352
BspMI ACCTGC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 144, 375
BssMI GATC 2 cut(s) 229, 535
BssNI GRCGYC 2 cut(s) 281, 369
BssT1I CCWWGG 2 cut(s) 144, 375
Bst2UI CCWGG 2 cut(s) 116, 224
Bst4CI ACNGT 1 cut(s) 568
Bst6I CTCTTC 1 cut(s) 86
BstACI GRCGYC 2 cut(s) 281, 369
BstC8I GCNNGC 2 cut(s) 217, 273
BstDEI CTNAG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 4 cut(s) 242, 275, 341, 493
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 2 cut(s) 232, 538
BstMAI GTCTC 2 cut(s) 245, 485
BstMBI GATC 2 cut(s) 229, 535
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNI CCWGG 2 cut(s) 116, 224
BstSCI CCNGG 2 cut(s) 114, 222
BstSLI GKGCMC 1 cut(s) 191
BstUI CGCG 4 cut(s) 242, 275, 341, 493
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BstZI CGGCCG 1 cut(s) 171
BsuRI GGCC 5 cut(s) 49, 149, 173, 353, 434
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 111
BtuMI TCGCGA 2 cut(s) 275, 341
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 2 cut(s) 217, 273
CciI TCATGA 1 cut(s) 309
CfoI GCGC 1 cut(s) 124
Cfr13I GGNCC 2 cut(s) 148, 351
CseI GACGC 2 cut(s) 289, 377
Csp6I GTAC 1 cut(s) 40
CviAII CATG 1 cut(s) 310
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 1 cut(s) 15
DpnI GATC 2 cut(s) 231, 537
DpnII GATC 2 cut(s) 229, 535
EaeI YGGCCR 2 cut(s) 171, 432
EagI CGGCCG 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EciI GGCGGA 1 cut(s) 202
EclXI CGGCCG 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 144, 375
Eco31I GGTCTC 1 cut(s) 485
Eco52I CGGCCG 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 72
Eco88I CYCGRG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 114, 222
EcoT14I CCWWGG 2 cut(s) 144, 375
ErhI CCWWGG 2 cut(s) 144, 375
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 1 cut(s) 313
FaiI YATR 5 cut(s) 101, 311, 426, 561, 583
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 166
FatI CATG 1 cut(s) 309
FauI CCCGC 1 cut(s) 340
Fnu4HI GCNGC 4 cut(s) 91, 120, 174, 372
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 4 cut(s) 91, 120, 174, 372
GlaI GCGC 1 cut(s) 123
GluI GCNGC 4 cut(s) 91, 120, 174, 372
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 5 cut(s) 49, 149, 173, 353, 434
HapII CCGG 1 cut(s) 456
HgaI GACGC 2 cut(s) 289, 377
HhaI GCGC 1 cut(s) 124
Hin1I GRCGYC 2 cut(s) 281, 369
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HinfI GANTC 1 cut(s) 19
HpaII CCGG 1 cut(s) 456
HphI GGTGA 4 cut(s) 325, 425, 457, 523
Hpy188I TCNGA 2 cut(s) 31, 467
Hpy188III TCNNGA 3 cut(s) 274, 310, 340
Hpy99I CGWCG 3 cut(s) 280, 283, 346
HpyAV CCTTC 4 cut(s) 219, 250, 346, 364
HpyCH4III ACNGT 1 cut(s) 568
HpyCH4IV ACGT 1 cut(s) 528
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpyF3I CTNAG 1 cut(s) 15
HpySE526I ACGT 1 cut(s) 528
Hsp92I GRCGYC 2 cut(s) 281, 369
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 1 cut(s) 122
KpnI GGTACC 1 cut(s) 43
Kzo9I GATC 2 cut(s) 229, 535
LweI GCATC 2 cut(s) 171, 258
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 3 cut(s) 331, 494, 529
MalI GATC 2 cut(s) 231, 537
MboI GATC 2 cut(s) 229, 535
MboII GAAGA 2 cut(s) 17, 73
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 6 cut(s) 57, 380, 437, 468, 520, 555
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 9 cut(s) 34, 77, 89, 216, 230, 241, 300, 403, 412
MseI TTAA 4 cut(s) 60, 74, 302, 383
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 2 cut(s) 116, 224
MvaI CCWGG 2 cut(s) 116, 224
MvnI CGCG 4 cut(s) 242, 275, 341, 493
MwoI GCNNNNNNNGC 1 cut(s) 96
NdeII GATC 2 cut(s) 229, 535
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 3 cut(s) 41, 150, 352
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 2 cut(s) 331, 529
NruI TCGCGA 2 cut(s) 275, 341
PagI TCATGA 1 cut(s) 309
PaqCI CACCTGC 1 cut(s) 401
PfeI GAWTC 1 cut(s) 19
PkrI GCNGC 4 cut(s) 92, 121, 175, 373
PshBI ATTAAT 1 cut(s) 383
PsiI TTATAA 1 cut(s) 561
Psp6I CCWGG 2 cut(s) 114, 222
PspGI CCWGG 2 cut(s) 114, 222
PspN4I GGNNCC 3 cut(s) 41, 150, 352
PspPI GGNCC 2 cut(s) 148, 351
PsuI RGATCY 1 cut(s) 229
RruI TCGCGA 2 cut(s) 275, 341
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SaqAI TTAA 4 cut(s) 60, 74, 302, 383
SatI GCNGC 4 cut(s) 91, 120, 174, 372
Sau3AI GATC 2 cut(s) 229, 535
Sau96I GGNCC 2 cut(s) 148, 351
ScrFI CCNGG 2 cut(s) 116, 224
SduI GDGCHC 1 cut(s) 191
SetI ASST 7 cut(s) 16, 338, 415, 453, 481, 510, 531
SfaNI GCATC 2 cut(s) 171, 258
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 6 cut(s) 57, 380, 437, 468, 520, 555
SsiI CCGC 8 cut(s) 90, 119, 160, 174, 213, 242, 347, 372
StyD4I CCNGG 2 cut(s) 114, 222
StyI CCWWGG 2 cut(s) 144, 375
TaaI ACNGT 1 cut(s) 568
TaiI ACGT 1 cut(s) 531
TasI AATT 6 cut(s) 57, 380, 437, 468, 520, 555
TauI GCSGC 4 cut(s) 93, 122, 176, 374
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 4 cut(s) 60, 74, 302, 383
Tru9I TTAA 4 cut(s) 60, 74, 302, 383
TseFI GTSAC 2 cut(s) 331, 529
Tsp45I GTSAC 2 cut(s) 331, 529
TspDTI ATGAA 1 cut(s) 326
VspI ATTAAT 1 cut(s) 383
XapI RAATTY 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.