pycom111g05010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0019307
Physical Location & Seq
Reverse (-)
3179093 .. 3179413
321 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g05010.1

Sequence Viewer

Length: 321 bp
ATGCTCGCGACGACGCCGTTGCCTCTTTTGGCTTTAACCATCATGAACCAGACCACTTTCAATGTCACCTTCGCGACGGCGGGGGCCTACGTCAGCGATGACGCCGCCAAGGAATTAATAATCTCCTCTAACTCCTCGTCAGCAGGTGATGGTTGTTATGGGTCGGCCAAATTGGGGGTGAAGCTGACCGGATATTTCAGAATTGATAAAGGTCTCTGGTTTTCGCGTTACTTCTGCGACCTTTCTCCCCCCAATTACAACGTCACCGATCATTCAAGCCAATGTGAAATTTATAATACTGTTTATCGGTGTGTATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

107

Amino Acids

11.56

Weight (kDa)

5.58

Isoelectric Point (pI)

22.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
AarI CACCTGC 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 134
AccII CGCG 3 cut(s) 8, 74, 226
AciI CCGC 2 cut(s) 80, 105
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 288
AcyI GRCGYC 2 cut(s) 14, 102
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 2 cut(s) 61, 276
AluBI AGCT 1 cut(s) 184
AluI AGCT 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 218
AoxI GGCC 2 cut(s) 84, 165
ApoI RAATTY 1 cut(s) 288
AseI ATTAAT 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 58, 158, 190, 256
BccI CCATC 2 cut(s) 47, 143
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 85
BsaHI GRCGYC 2 cut(s) 14, 102
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 115, 124
Bsh1236I CGCG 3 cut(s) 8, 74, 226
BshFI GGCC 2 cut(s) 86, 167
BsiSI CCGG 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 218
BsnI GGCC 2 cut(s) 86, 167
Bso31I GGTCTC 1 cut(s) 218
Bsp143I GATC 1 cut(s) 268
Bsp68I TCGCGA 2 cut(s) 8, 74
BspACI CCGC 2 cut(s) 80, 105
BspANI GGCC 2 cut(s) 86, 167
BspFNI CGCG 3 cut(s) 8, 74, 226
BspHI TCATGA 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 1 cut(s) 268
BssNI GRCGYC 2 cut(s) 14, 102
BssT1I CCWWGG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 301
BstACI GRCGYC 2 cut(s) 14, 102
BstC8I GCNNGC 1 cut(s) 6
BstFNI CGCG 3 cut(s) 8, 74, 226
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 1 cut(s) 268
BstUI CGCG 3 cut(s) 8, 74, 226
BsuRI GGCC 2 cut(s) 86, 167
BtgZI GCGATG 1 cut(s) 111
BtuMI TCGCGA 2 cut(s) 8, 74
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 84
CseI GACGC 2 cut(s) 22, 110
CviAII CATG 1 cut(s) 43
CviJI RGCY 5 cut(s) 32, 86, 167, 184, 279
CviKI_1 RGCY 5 cut(s) 32, 86, 167, 184, 279
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 108
Eco31I GGTCTC 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 1 cut(s) 46
FaiI YATR 4 cut(s) 44, 159, 294, 316
FatI CATG 1 cut(s) 42
FauI CCCGC 1 cut(s) 73
Fnu4HI GCNGC 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 105
GluI GCNGC 1 cut(s) 105
HaeIII GGCC 2 cut(s) 86, 167
HapII CCGG 1 cut(s) 189
HgaI GACGC 2 cut(s) 22, 110
Hin1I GRCGYC 2 cut(s) 14, 102
Hin1II CATG 1 cut(s) 46
HpaII CCGG 1 cut(s) 189
HphI GGTGA 4 cut(s) 58, 158, 190, 256
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 3 cut(s) 7, 43, 73
Hpy99I CGWCG 3 cut(s) 13, 16, 79
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4IV ACGT 2 cut(s) 90, 261
HpySE526I ACGT 2 cut(s) 90, 261
Hsp92I GRCGYC 2 cut(s) 14, 102
Hsp92II CATG 1 cut(s) 46
Kzo9I GATC 1 cut(s) 268
LpnPI CCDG 4 cut(s) 62, 129, 202, 202
MaeII ACGT 2 cut(s) 90, 261
MaeIII GTNAC 3 cut(s) 64, 227, 262
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MluCI AATT 5 cut(s) 113, 170, 201, 253, 288
MnlI CCTC 3 cut(s) 33, 136, 145
MseI TTAA 2 cut(s) 35, 116
MspI CCGG 1 cut(s) 189
MvnI CGCG 3 cut(s) 8, 74, 226
NdeII GATC 1 cut(s) 268
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 64, 262
NruI TCGCGA 2 cut(s) 8, 74
PagI TCATGA 1 cut(s) 42
PaqCI CACCTGC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 116
PsiI TTATAA 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 84
RruI TCGCGA 2 cut(s) 8, 74
SaqAI TTAA 2 cut(s) 35, 116
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 1 cut(s) 84
SetI ASST 7 cut(s) 71, 93, 148, 186, 214, 243, 264
Sse9I AATT 5 cut(s) 113, 170, 201, 253, 288
SsiI CCGC 2 cut(s) 80, 105
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 301
TaiI ACGT 2 cut(s) 93, 264
TasI AATT 5 cut(s) 113, 170, 201, 253, 288
TauI GCSGC 1 cut(s) 107
Tru1I TTAA 2 cut(s) 35, 116
Tru9I TTAA 2 cut(s) 35, 116
TseFI GTSAC 2 cut(s) 64, 262
Tsp45I GTSAC 2 cut(s) 64, 262
TspDTI ATGAA 1 cut(s) 59
VspI ATTAAT 1 cut(s) 116
XapI RAATTY 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.