pycom111g05380

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0015224
Physical Location & Seq
Forward (+)
3449313 .. 3452109
2797 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGGAGGAGGTTGAGAAACAACCCGATGAAGTCATTTTTGACCATCTGCATGCAACTGCATTCCAGTACTCTCCTCTAGCCAGAACTATTCTTGGACCGTCTGGCAACATTAAGACCATCTCGAAAGAGCATCTTCAGAACTATATCAAAACACATTACACTGCTCCCAGAATGGTTATTGCTGCTTCAGGAGCTATTAAGCATGAAGATCTTGTTGGTTCAGTTAAGGGTTTGTTTACTAAACTGTCCGGAGATCCTGCCACAGCCAGTCAGTTGGTTGCGGAAGAACCTTCATTTTTCACTGGTTCTGAGGTCAGAATTGTGGATGATGACCTGCCCTTGGCACATTTTGCGGTTGCATTCAGTGGAGCATCTTGGACAGATCCAGACTCTATTCCTCTAATGGTCATGCAGGCAATGTTGGGCTCATGGAATAAAAATGTTGGTGGTGGAAAGCACATGGGTTCAGAACTGGCACAAAGGGTTGCCATCAATGACATTGCAGAAAACTATATGGCTTTCAACACCAACTATAAGGATACTGGTTTATTTGGTGTTTTTGCTACTGCTAAGGAGGATTGCTTAGATGATTTGGCCTATGCAATAATGTATGAGGTAACCAAGTTAGTTTATCGAGTTTCAGAAGCTGATGTTATTCGTGCGCGCAATCAGTTGAAATCTTCTCTCCTTCTCCACTTGGATGGGACAAGTGCTGTAGCTGAAGATATTGGACGCCAGCTACTTACATATGGTCGAAGAATCCCATTAGCCGAGATGTTTGCTAGAATCGATTCTGTTGATGCAAGCACCATCAAACGTGTTGCAAACCGGTTTATCTATGACAGGTGCAATTGCAGCCATGGGTCCCATTACAAAATTGCCCGACTACAACTGGTTCAGACGCCGCACCTACTGGAACAGATACTAAGCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

312

Amino Acids

34.35

Weight (kDa)

5.88

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_C PF05193 40 - 225 2.8e-36 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 342
AccII CGCG 1 cut(s) 664
AccIII TCCGGA 1 cut(s) 248
AciI CCGC 3 cut(s) 281, 353, 905
AclWI GGATC 2 cut(s) 248, 377
AcuI CTGAAG 3 cut(s) 119, 171, 741
AcyI GRCGYC 2 cut(s) 733, 902
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 1 cut(s) 340
AflIII ACRYGT 1 cut(s) 817
AgeI ACCGGT 1 cut(s) 828
AgsI TTSAA 2 cut(s) 523, 676
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AluBI AGCT 5 cut(s) 194, 647, 719, 739, 930
AluI AGCT 5 cut(s) 194, 647, 719, 739, 930
AlwI GGATC 2 cut(s) 248, 377
AlwNI CAGNNNCTG 1 cut(s) 647
Aor13HI TCCGGA 1 cut(s) 248
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 2 cut(s) 182, 855
AsiGI ACCGGT 1 cut(s) 828
Asp700I GAANNNNTTC 1 cut(s) 790
AspLEI GCGC 2 cut(s) 664, 666
AspS9I GGNCC 2 cut(s) 95, 864
AvaII GGWCC 2 cut(s) 95, 864
BanII GRGCYC 1 cut(s) 428
BbvI GCAGC 2 cut(s) 169, 867
BccI CCATC 5 cut(s) 51, 125, 497, 695, 818
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciVI GTATCC 1 cut(s) 532
BfaI CTAG 3 cut(s) 77, 783, 934
BfmI CTRYAG 1 cut(s) 714
BfuAI ACCTGC 1 cut(s) 342
BfuI GTATCC 1 cut(s) 532
BglII AGATCT 1 cut(s) 208
BisI GCNGC 3 cut(s) 183, 856, 905
BlsI GCNGC 3 cut(s) 184, 857, 906
BmcAI AGTACT 1 cut(s) 68
Bme18I GGWCC 2 cut(s) 95, 864
BmgT120I GGNCC 2 cut(s) 95, 864
BmiI GGNNCC 2 cut(s) 865, 866
BmsI GCATC 3 cut(s) 139, 380, 790
Bpu10I CCTNAGC 1 cut(s) 570
Bsa29I ATCGAT 1 cut(s) 789
BsaHI GRCGYC 2 cut(s) 733, 902
BsaJI CCNNGG 2 cut(s) 339, 859
BsaWI WCCGGW 2 cut(s) 248, 828
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse118I RCCGGY 1 cut(s) 828
Bse1I ACTGG 7 cut(s) 64, 267, 307, 477, 547, 897, 918
Bse3DI GCAATG 2 cut(s) 423, 498
BseAI TCCGGA 1 cut(s) 248
BseCI ATCGAT 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 339, 859
BseGI GGATG 2 cut(s) 331, 706
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMI GCAATG 2 cut(s) 423, 498
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 7 cut(s) 64, 267, 307, 477, 547, 897, 918
BsePI GCGCGC 1 cut(s) 662
BseRI GAGGAG 2 cut(s) 20, 63
BseXI GCAGC 2 cut(s) 169, 867
Bsh1236I CGCG 1 cut(s) 664
BshFI GGCC 1 cut(s) 596
BshTI ACCGGT 1 cut(s) 828
BshVI ATCGAT 1 cut(s) 789
BsiSI CCGG 2 cut(s) 249, 829
BslFI GGGAC 2 cut(s) 718, 850
BslI CCNNNNNNNGG 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 718, 850
BsmI GAATGC 2 cut(s) 59, 359
BsnI GGCC 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 428
Bsp13I TCCGGA 1 cut(s) 248
Bsp143I GATC 3 cut(s) 208, 253, 382
Bsp19I CCATGG 1 cut(s) 859
BspACI CCGC 3 cut(s) 281, 353, 905
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 1 cut(s) 301
BspDI ATCGAT 1 cut(s) 789
BspEI TCCGGA 1 cut(s) 248
BspFNI CGCG 1 cut(s) 664
BspLI GGNNCC 2 cut(s) 865, 866
BspMI ACCTGC 1 cut(s) 342
BspPI GGATC 2 cut(s) 248, 377
BsrDI GCAATG 2 cut(s) 423, 498
BsrFI RCCGGY 1 cut(s) 828
BsrI ACTGG 7 cut(s) 64, 267, 307, 477, 547, 897, 918
BssAI RCCGGY 1 cut(s) 828
BssECI CCNNGG 2 cut(s) 339, 859
BssHII GCGCGC 1 cut(s) 662
BssMI GATC 3 cut(s) 208, 253, 382
BssNI GRCGYC 2 cut(s) 733, 902
BssT1I CCWWGG 2 cut(s) 339, 859
Bst4CI ACNGT 2 cut(s) 99, 246
BstACI GRCGYC 2 cut(s) 733, 902
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 5 cut(s) 51, 414, 664, 737, 805
BstDEI CTNAG 4 cut(s) 309, 570, 583, 926
BstDSI CCRYGG 1 cut(s) 859
BstEII GGTNACC 1 cut(s) 616
BstF5I GGATG 2 cut(s) 331, 706
BstFNI CGCG 1 cut(s) 664
BstHHI GCGC 2 cut(s) 664, 666
BstKTI GATC 3 cut(s) 211, 256, 385
BstMBI GATC 3 cut(s) 208, 253, 382
BstMWI GCNNNNNNNGC 3 cut(s) 191, 350, 855
BstNSI RCATGY 1 cut(s) 53
BstPI GGTNACC 1 cut(s) 616
BstSFI CTRYAG 1 cut(s) 714
BstUI CGCG 1 cut(s) 664
BstV1I GCAGC 2 cut(s) 169, 867
BstX2I RGATCY 3 cut(s) 208, 253, 382
BstXI CCANNNNNNTGG 2 cut(s) 274, 701
BstYI RGATCY 3 cut(s) 208, 253, 382
Bsu15I ATCGAT 1 cut(s) 789
BsuI GTATCC 1 cut(s) 532
BsuRI GGCC 1 cut(s) 596
BsuTUI ATCGAT 1 cut(s) 789
BtgI CCRYGG 1 cut(s) 859
BtsCI GGATG 2 cut(s) 331, 706
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 3 cut(s) 159, 300, 370
BveI ACCTGC 1 cut(s) 342
Cac8I GCNNGC 5 cut(s) 51, 414, 664, 737, 805
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 2 cut(s) 664, 666
Cfr10I RCCGGY 1 cut(s) 828
Cfr13I GGNCC 2 cut(s) 95, 864
ClaI ATCGAT 1 cut(s) 789
CseI GACGC 2 cut(s) 741, 910
Csp6I GTAC 1 cut(s) 67
CspAI ACCGGT 1 cut(s) 828
CviAII CATG 6 cut(s) 50, 203, 409, 429, 460, 860
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 4 cut(s) 309, 570, 583, 926
DpnI GATC 3 cut(s) 210, 255, 384
DpnII GATC 3 cut(s) 208, 253, 382
Eco130I CCWWGG 2 cut(s) 339, 859
Eco24I GRGCYC 1 cut(s) 428
Eco47I GGWCC 2 cut(s) 95, 864
Eco57I CTGAAG 3 cut(s) 119, 171, 741
Eco91I GGTNACC 1 cut(s) 616
EcoO109I RGGNCCY 1 cut(s) 864
EcoO65I GGTNACC 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 339, 859
EcoT38I GRGCYC 1 cut(s) 428
ErhI CCWWGG 2 cut(s) 339, 859
FaeI CATG 6 cut(s) 53, 206, 412, 432, 463, 863
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FaqI GGGAC 2 cut(s) 718, 850
FatI CATG 6 cut(s) 49, 202, 408, 428, 459, 859
FauNDI CATATG 1 cut(s) 748
Fnu4HI GCNGC 3 cut(s) 183, 856, 905
FokI GGATG 2 cut(s) 338, 713
FriOI GRGCYC 1 cut(s) 428
Fsp4HI GCNGC 3 cut(s) 183, 856, 905
FspBI CTAG 3 cut(s) 77, 783, 934
GlaI GCGC 2 cut(s) 663, 665
GluI GCNGC 3 cut(s) 183, 856, 905
HaeIII GGCC 1 cut(s) 596
HapII CCGG 2 cut(s) 249, 829
HgaI GACGC 2 cut(s) 741, 910
HhaI GCGC 2 cut(s) 664, 666
Hin1I GRCGYC 2 cut(s) 733, 902
Hin1II CATG 6 cut(s) 53, 206, 412, 432, 463, 863
Hin6I GCGC 2 cut(s) 662, 664
HinP1I GCGC 2 cut(s) 662, 664
HinfI GANTC 4 cut(s) 389, 759, 786, 791
HpaII CCGG 2 cut(s) 249, 829
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 6 cut(s) 138, 310, 317, 469, 643, 900
Hpy188III TCNNGA 4 cut(s) 121, 189, 249, 386
Hpy8I GTNNAC 1 cut(s) 237
HpyAV CCTTC 2 cut(s) 300, 698
HpyCH4III ACNGT 2 cut(s) 99, 246
HpyCH4IV ACGT 1 cut(s) 817
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 350, 855
HpyF3I CTNAG 4 cut(s) 309, 570, 583, 926
HpySE526I ACGT 1 cut(s) 817
Hsp92I GRCGYC 2 cut(s) 733, 902
Hsp92II CATG 6 cut(s) 53, 206, 412, 432, 463, 863
HspAI GCGC 2 cut(s) 662, 664
KflI GGGWCCC 1 cut(s) 864
Kpn2I TCCGGA 1 cut(s) 248
Kzo9I GATC 3 cut(s) 208, 253, 382
LmnI GCTCC 4 cut(s) 169, 191, 368, 935
Lsp1109I GCAGC 2 cut(s) 169, 867
LweI GCATC 3 cut(s) 139, 380, 790
MaeI CTAG 3 cut(s) 77, 783, 934
MaeII ACGT 1 cut(s) 817
MaeIII GTNAC 1 cut(s) 616
MalI GATC 3 cut(s) 210, 255, 384
MboI GATC 3 cut(s) 208, 253, 382
MboII GAAGA 6 cut(s) 125, 218, 296, 672, 734, 768
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 3 cut(s) 208, 253, 382
MhlI GDGCHC 1 cut(s) 428
MluCI AATT 3 cut(s) 318, 850, 876
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 5 cut(s) 84, 304, 408, 568, 607
MroI TCCGGA 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 790
MseI TTAA 3 cut(s) 111, 198, 225
MslI CAYNNNNRTG 2 cut(s) 48, 699
MspI CCGG 2 cut(s) 249, 829
MunI CAATTG 1 cut(s) 850
Mva1269I GAATGC 2 cut(s) 59, 359
MvnI CGCG 1 cut(s) 664
MwoI GCNNNNNNNGC 3 cut(s) 191, 350, 855
NcoI CCATGG 1 cut(s) 859
NdeI CATATG 1 cut(s) 748
NdeII GATC 3 cut(s) 208, 253, 382
NlaIII CATG 6 cut(s) 53, 206, 412, 432, 463, 863
NlaIV GGNNCC 2 cut(s) 865, 866
NmeAIII GCCGAG 1 cut(s) 796
NspI RCATGY 1 cut(s) 53
PaeI GCATGC 1 cut(s) 53
PauI GCGCGC 1 cut(s) 662
PctI GAATGC 2 cut(s) 59, 359
PdmI GAANNNNTTC 1 cut(s) 790
PfeI GAWTC 3 cut(s) 759, 786, 791
PinAI ACCGGT 1 cut(s) 828
PkrI GCNGC 3 cut(s) 184, 857, 906
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PpuMI RGGWCCY 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 864
PspEI GGTNACC 1 cut(s) 616
PspN4I GGNNCC 2 cut(s) 865, 866
PspPI GGNCC 2 cut(s) 95, 864
PspPPI RGGWCCY 1 cut(s) 864
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 3 cut(s) 208, 253, 382
PteI GCGCGC 1 cut(s) 662
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
RseI CAYNNNNRTG 2 cut(s) 48, 699
SaqAI TTAA 3 cut(s) 111, 198, 225
SatI GCNGC 3 cut(s) 183, 856, 905
Sau3AI GATC 3 cut(s) 208, 253, 382
Sau96I GGNCC 2 cut(s) 95, 864
ScaI AGTACT 1 cut(s) 68
SchI GAGTC 1 cut(s) 383
SduI GDGCHC 1 cut(s) 428
SfaNI GCATC 3 cut(s) 139, 380, 790
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 2 cut(s) 95, 864
SmiMI CAYNNNNRTG 2 cut(s) 48, 699
SphI GCATGC 1 cut(s) 53
Sse9I AATT 3 cut(s) 318, 850, 876
SsiI CCGC 3 cut(s) 281, 353, 905
SspMI CTAG 3 cut(s) 77, 783, 934
StyI CCWWGG 2 cut(s) 339, 859
TaaI ACNGT 2 cut(s) 99, 246
TaiI ACGT 1 cut(s) 820
TaqI TCGA 4 cut(s) 122, 634, 754, 789
TasI AATT 3 cut(s) 318, 850, 876
TatI WGTACW 1 cut(s) 66
TauI GCSGC 1 cut(s) 907
TfiI GAWTC 3 cut(s) 759, 786, 791
Tru1I TTAA 3 cut(s) 111, 198, 225
Tru9I TTAA 3 cut(s) 111, 198, 225
TscAI CASTG 3 cut(s) 166, 307, 370
TseI GCWGC 2 cut(s) 182, 855
TspDTI ATGAA 3 cut(s) 42, 219, 282
TspRI CASTG 3 cut(s) 166, 307, 370
VpaK11BI GGWCC 2 cut(s) 95, 864
XceI RCATGY 1 cut(s) 53
XmnI GAANNNNTTC 1 cut(s) 790
XspI CTAG 3 cut(s) 77, 783, 934
ZrmI AGTACT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.