pycom11g04880

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0014200
Physical Location & Seq
Forward (+)
3773007 .. 3773615
609 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
ATGAGTTTCAATAATTCCTATATATGTATCACACAGCTATTCCAAACCTTCCATAGCATTCAAGCTTTCATCAACCCAAAAACCCCAAAGAAACACAAAAGAAAACCTAAAAACAAATCAAAATCCACAGAGAGAGCTCAAAAACCAGAGATGGGCAACAGTCTAAGGTGCTGTTTGGCTTGCGTACTACCATGTGGAGCCCTAGACTTGATCCGGATCGTCCATTTGAATGGCTACGTAGAAGAAATCTCACATCCAGTTACAGCCGGCGAGATCTTAGAAGCAAACCCTAATCACCTTCTCAGCAAACCCAGCTGCCAAGGGGTCGTGCGCAAAATTTTGATCCTCTCGCCGGAGTCGGAGCTCAAGCGAGGCAGCATCTATTTTTTGATTCCGTCTTCTTCTGTGCCGGAGAAGAAGAAGAAGAAGAAGAGTAAGAGCAGCGGCAATAAGAAACCCTCAAAAAAGAGCAGCACGAGCCAAAGCGATAGTACCGGCCGTTACTTGACGGAGATTGTGACGGAAAAGAAATCTATGTCTCGCCAAGATAGGAGGGCTGGCCGTGTCACGGTATGGCGGCCTCATCTCGAAAGCATCACCGAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

203

Amino Acids

22.63

Weight (kDa)

10.0

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 51 - 201 4.2e-39 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06980 AT2G30230
fragaria_vesca FvH4_3g39820
malus_domestica MD03G1057000.v1.1 MD11G1058700.v1.1
prunus_persica Prupe.6G047800_v2.0.a1
pyrus_communis pycom03g04530 pycom11g04880
rosa_chinensis RchiOBHm_Chr5g0071711
rosa_laevigata RLG00000036243
rosa_multiflora Rmu_sc0022933.1_g000001
rosa_roxburghii Rroxscaffold_1G00009470
rosa_rugosa Rorug05G0414300
rosa_samantha Rh5BG490000 Rh5CG514000 Rh5DG500800
rosa_wichuraiana Rw5G043710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 332
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 2 cut(s) 444, 577
AclWI GGATC 3 cut(s) 205, 224, 337
AcoI YGGCCR 2 cut(s) 496, 559
AcsI RAATTY 1 cut(s) 336
AfaI GTAC 2 cut(s) 186, 493
AfiI CCNNNNNNNGG 3 cut(s) 152, 352, 568
AgsI TTSAA 3 cut(s) 10, 62, 229
AluBI AGCT 5 cut(s) 37, 65, 137, 315, 364
AluI AGCT 5 cut(s) 37, 65, 137, 315, 364
Alw21I GWGCWC 2 cut(s) 139, 366
Alw26I GTCTC 1 cut(s) 543
AlwI GGATC 3 cut(s) 205, 224, 337
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 3 cut(s) 496, 559, 578
ApeKI GCWGC 4 cut(s) 315, 375, 441, 471
ApoI RAATTY 1 cut(s) 336
AspLEI GCGC 1 cut(s) 333
AsuHPI GGTGA 2 cut(s) 287, 589
BanII GRGCYC 3 cut(s) 139, 202, 366
BauI CACGAG 1 cut(s) 475
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 139, 366
BbvI GCAGC 4 cut(s) 302, 387, 453, 483
BccI CCATC 1 cut(s) 145
BceAI ACGGC 2 cut(s) 483, 546
BcoDI GTCTC 1 cut(s) 543
BfaI CTAG 1 cut(s) 203
BglII AGATCT 1 cut(s) 273
BisI GCNGC 6 cut(s) 316, 376, 442, 445, 472, 578
BlsI GCNGC 6 cut(s) 317, 377, 443, 446, 473, 579
BmiI GGNNCC 1 cut(s) 199
BmsI GCATC 2 cut(s) 387, 603
BpiI GAAGAC 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 319
BsaWI WCCGGW 1 cut(s) 213
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 352, 568
Bse118I RCCGGY 2 cut(s) 266, 494
Bse1I ACTGG 1 cut(s) 257
Bse8I GATNNNNATC 1 cut(s) 215
BseAI TCCGGA 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 1 cut(s) 253
BseJI GATNNNNATC 1 cut(s) 215
BseLI CCNNNNNNNGG 3 cut(s) 152, 352, 568
BseMII CTCAG 1 cut(s) 316
BseNI ACTGG 1 cut(s) 257
BseX3I CGGCCG 1 cut(s) 496
BseXI GCAGC 4 cut(s) 302, 387, 453, 483
BseYI CCCAGC 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 499
BshFI GGCC 3 cut(s) 498, 561, 580
BsiEI CGRYCG 1 cut(s) 499
BsiHKAI GWGCWC 2 cut(s) 139, 366
BsiSI CCGG 5 cut(s) 214, 267, 353, 410, 495
BslI CCNNNNNNNGG 3 cut(s) 152, 352, 568
BsmAI GTCTC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 57
BsnI GGCC 3 cut(s) 498, 561, 580
Bsp1286I GDGCHC 3 cut(s) 139, 202, 366
Bsp13I TCCGGA 1 cut(s) 213
Bsp143I GATC 4 cut(s) 210, 216, 273, 342
BspACI CCGC 2 cut(s) 444, 577
BspANI GGCC 3 cut(s) 498, 561, 580
BspCNI CTCAG 1 cut(s) 315
BspEI TCCGGA 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 199
BspPI GGATC 3 cut(s) 205, 224, 337
BsrFI RCCGGY 2 cut(s) 266, 494
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 2 cut(s) 266, 494
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 4 cut(s) 210, 216, 273, 342
BssSI CACGAG 1 cut(s) 475
BssT1I CCWWGG 1 cut(s) 319
Bst2BI CACGAG 1 cut(s) 475
Bst4CI ACNGT 2 cut(s) 161, 571
Bst6I CTCTTC 1 cut(s) 425
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 181, 268, 559
BstDEI CTNAG 3 cut(s) 164, 277, 302
BstF5I GGATG 1 cut(s) 253
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 4 cut(s) 213, 219, 276, 345
BstMAI GTCTC 1 cut(s) 543
BstMBI GATC 4 cut(s) 210, 216, 273, 342
BstMCI CGRYCG 1 cut(s) 499
BstMWI GCNNNNNNNGC 2 cut(s) 312, 477
BstSNI TACGTA 1 cut(s) 238
BstV1I GCAGC 4 cut(s) 302, 387, 453, 483
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 1 cut(s) 273
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 1 cut(s) 273
BstZI CGGCCG 1 cut(s) 496
BsuRI GGCC 3 cut(s) 498, 561, 580
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 3 cut(s) 181, 268, 559
CfoI GCGC 1 cut(s) 333
Cfr10I RCCGGY 2 cut(s) 266, 494
Csp6I GTAC 2 cut(s) 185, 492
CviAII CATG 1 cut(s) 192
CviQI GTAC 2 cut(s) 185, 492
DdeI CTNAG 3 cut(s) 164, 277, 302
DpnI GATC 4 cut(s) 212, 218, 275, 344
DpnII GATC 4 cut(s) 210, 216, 273, 342
EaeI YGGCCR 2 cut(s) 496, 559
EagI CGGCCG 1 cut(s) 496
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Ecl136II GAGCTC 2 cut(s) 137, 364
EclXI CGGCCG 1 cut(s) 496
Eco105I TACGTA 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 3 cut(s) 139, 202, 366
Eco52I CGGCCG 1 cut(s) 496
Eco53kI GAGCTC 2 cut(s) 137, 364
EcoICRI GAGCTC 2 cut(s) 137, 364
EcoT14I CCWWGG 1 cut(s) 319
EcoT38I GRGCYC 3 cut(s) 139, 202, 366
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 1 cut(s) 195
FaiI YATR 7 cut(s) 21, 23, 25, 54, 193, 536, 574
FatI CATG 1 cut(s) 191
Fnu4HI GCNGC 6 cut(s) 316, 376, 442, 445, 472, 578
FokI GGATG 1 cut(s) 240
FriOI GRGCYC 3 cut(s) 139, 202, 366
Fsp4HI GCNGC 6 cut(s) 316, 376, 442, 445, 472, 578
FspBI CTAG 1 cut(s) 203
FspI TGCGCA 1 cut(s) 332
GlaI GCGC 1 cut(s) 332
GluI GCNGC 6 cut(s) 316, 376, 442, 445, 472, 578
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 3 cut(s) 498, 561, 580
HapII CCGG 5 cut(s) 214, 267, 353, 410, 495
HhaI GCGC 1 cut(s) 333
Hin1II CATG 1 cut(s) 195
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 2 cut(s) 356, 391
HpaII CCGG 5 cut(s) 214, 267, 353, 410, 495
HphI GGTGA 2 cut(s) 287, 589
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 2 cut(s) 214, 587
HpyAV CCTTC 3 cut(s) 58, 308, 596
HpyCH4III ACNGT 2 cut(s) 161, 571
HpyCH4IV ACGT 1 cut(s) 237
HpyF10VI GCNNNNNNNGC 2 cut(s) 312, 477
HpyF3I CTNAG 3 cut(s) 164, 277, 302
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 1 cut(s) 195
HspAI GCGC 1 cut(s) 331
Kpn2I TCCGGA 1 cut(s) 213
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Kzo9I GATC 4 cut(s) 210, 216, 273, 342
LmnI GCTCC 2 cut(s) 197, 361
LpnPI CCDG 9 cut(s) 159, 227, 270, 280, 325, 366, 423, 508, 543
Lsp1109I GCAGC 4 cut(s) 302, 387, 453, 483
LweI GCATC 2 cut(s) 387, 603
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 4 cut(s) 259, 500, 517, 565
MalI GATC 4 cut(s) 212, 218, 275, 344
MboI GATC 4 cut(s) 210, 216, 273, 342
MboII GAAGA 9 cut(s) 254, 390, 393, 427, 430, 433, 436, 439, 442
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 3 cut(s) 139, 202, 366
MluCI AATT 2 cut(s) 13, 336
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 5 cut(s) 356, 365, 469, 546, 591
MroI TCCGGA 1 cut(s) 213
MroNI GCCGGC 1 cut(s) 266
MslI CAYNNNNRTG 1 cut(s) 228
MspA1I CMGCKG 2 cut(s) 315, 444
MspI CCGG 5 cut(s) 214, 267, 353, 410, 495
Mva1269I GAATGC 1 cut(s) 57
MwoI GCNNNNNNNGC 2 cut(s) 312, 477
NaeI GCCGGC 1 cut(s) 268
NdeII GATC 4 cut(s) 210, 216, 273, 342
NgoMIV GCCGGC 1 cut(s) 266
NlaIII CATG 1 cut(s) 195
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 517, 565
NsbI TGCGCA 1 cut(s) 332
PctI GAATGC 1 cut(s) 57
PdiI GCCGGC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 6 cut(s) 317, 377, 443, 446, 473, 579
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
Ppu21I YACGTR 1 cut(s) 238
Psp124BI GAGCTC 2 cut(s) 139, 366
PspFI CCCAGC 1 cut(s) 311
PspN4I GGNNCC 1 cut(s) 199
PsuI RGATCY 1 cut(s) 273
PvuII CAGCTG 1 cut(s) 315
RsaI GTAC 2 cut(s) 186, 493
RsaNI GTAC 2 cut(s) 185, 492
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 2 cut(s) 139, 366
SatI GCNGC 6 cut(s) 316, 376, 442, 445, 472, 578
Sau3AI GATC 4 cut(s) 210, 216, 273, 342
SchI GAGTC 1 cut(s) 365
SduI GDGCHC 3 cut(s) 139, 202, 366
SfaNI GCATC 2 cut(s) 387, 603
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SnaBI TACGTA 1 cut(s) 238
Sse9I AATT 2 cut(s) 13, 336
SsiI CCGC 2 cut(s) 444, 577
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 2 cut(s) 139, 366
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 161, 571
TaiI ACGT 1 cut(s) 240
TaqI TCGA 1 cut(s) 588
TasI AATT 2 cut(s) 13, 336
TauI GCSGC 2 cut(s) 447, 580
TfiI GAWTC 1 cut(s) 391
TseFI GTSAC 2 cut(s) 517, 565
TseI GCWGC 4 cut(s) 315, 375, 441, 471
Tsp45I GTSAC 2 cut(s) 517, 565
TspDTI ATGAA 1 cut(s) 58
TspGWI ACGGA 3 cut(s) 384, 524, 536
XapI RAATTY 1 cut(s) 336
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.