pycom11g06260

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007024
Physical Location & Seq
Forward (+)
4815184 .. 4817474
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
ATGGGAAAACCGTTCAGGGACTCTCTTAAAGCGCTTCAAGCAGATATTCAGCACGCCAATACTCTGGCACTGGATTATCCGAGGGAGAAAGACGGAGCACGTCTTCAGATGAGACTGTCTTACTGTCCGGCGGCCAACTTTTTCCTCTTTCTAGTCCAGTGGACTGACTGTCACCTCGCCGGAGCACTCGGCTTGCTTCGAATTCTTATTTATATGACTTATCCTGATGGAAAGACCACCATGTCTGTTTACGAAAGGAAAGCCAGCATGAGAGAGTTTTATGCTGTCATATTTCCTTCTTTGTTGCAACTTCAAAGGGGAATCACAGATGTGGAGGAGAGGAAACAGAAAGAGATTTGCACAGCCAGATACAAAAGAAGGGATGAATCCGAGAGGGGAAAACTCTCTGAAATTGAGATTGAAAGAGAAGAAGAGTGTGGAATTTGCATGGAGGTGAATAGAAAAGTCGTGTTGCCTAACTGCAGTCACACATTGTGTTTGAAGTGTTATCGAGACTGGCGTGGTAGGTCTCAGTCATGCCCCTTTTGTCGCGATAGTCTCAAAAGAATCAACTCAGGTGACCTTTGGATCTACACTGAGAAATGCGATGCTCTCGATTTATCATCAATCTTAAGAGAGGACCGCAAGAGACTATTTATGTACGTAGAAAAACTGCCTCTTGTTATGCCAGATCCTGTTTTTCTACCCTATGATTCTCATGTCCGGTGA

Protein Analysis

243

Amino Acids

28.37

Weight (kDa)

8.59

Isoelectric Point (pI)

64.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 143 - 189 1.7e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 145 - 184 9.4e-09 Ring finger domain
zf-RING_5 PF14634 146 - 184 2.5e-06 zinc-RING finger domain
Prok-RING_4 PF14447 146 - 187 9.9e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 241
AccII CGCG 1 cut(s) 552
AciI CCGC 2 cut(s) 131, 643
AclWI GGATC 2 cut(s) 596, 686
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 2 cut(s) 201, 441
AcuI CTGAAG 1 cut(s) 89
AdeI CACNNNGTG 1 cut(s) 495
AfaI GTAC 1 cut(s) 662
AfeI AGCGCT 1 cut(s) 33
AflII CTTAAG 1 cut(s) 631
AgsI TTSAA 4 cut(s) 38, 314, 422, 502
AhdI GACNNNNNGTC 1 cut(s) 168
AjiI CACGTC 1 cut(s) 101
AleI CACNNNNGTG 1 cut(s) 329
Alw21I GWGCWC 2 cut(s) 100, 187
Alw26I GTCTC 5 cut(s) 106, 507, 534, 563, 643
AlwI GGATC 2 cut(s) 596, 686
AlwNI CAGNNNCTG 1 cut(s) 695
Aor51HI AGCGCT 1 cut(s) 33
AoxI GGCC 1 cut(s) 132
ApoI RAATTY 2 cut(s) 201, 441
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 640
AsuHPI GGTGA 3 cut(s) 164, 466, 590
AsuII TTCGAA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 640
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 2 cut(s) 100, 187
BccI CCATC 1 cut(s) 221
BcoDI GTCTC 5 cut(s) 106, 507, 534, 563, 643
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 481
BfoI RGCGCY 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 631
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 640
BmeRI GACNNNNNGTC 1 cut(s) 168
BmgBI CACGTC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 640
BmsI GCATC 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 95
Bpu14I TTCGAA 1 cut(s) 199
BsaAI YACGTR 1 cut(s) 664
BsaI GGTCTC 1 cut(s) 534
BsaJI CCNNGG 1 cut(s) 80
BsaWI WCCGGW 1 cut(s) 723
Bse1I ACTGG 3 cut(s) 75, 157, 521
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 388
BseMII CTCAG 3 cut(s) 545, 588, 588
BseNI ACTGG 3 cut(s) 75, 157, 521
BseRI GAGGAG 1 cut(s) 350
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 100, 187
BsiSI CCGG 3 cut(s) 128, 180, 724
BslFI GGGAC 1 cut(s) 32
BsmAI GTCTC 5 cut(s) 106, 507, 534, 563, 643
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 1 cut(s) 134
Bso31I GGTCTC 1 cut(s) 534
Bsp119I TTCGAA 1 cut(s) 199
Bsp1286I GDGCHC 2 cut(s) 100, 187
Bsp143I GATC 2 cut(s) 588, 691
Bsp68I TCGCGA 1 cut(s) 552
BspACI CCGC 2 cut(s) 131, 643
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 3 cut(s) 544, 587, 589
BspFNI CGCG 1 cut(s) 552
BspMAI CTGCAG 1 cut(s) 485
BspPI GGATC 2 cut(s) 596, 686
BspT104I TTCGAA 1 cut(s) 199
BspTI CTTAAG 1 cut(s) 631
BspTNI GGTCTC 1 cut(s) 534
BsrI ACTGG 3 cut(s) 75, 157, 521
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 2 cut(s) 588, 691
Bst4CI ACNGT 4 cut(s) 12, 117, 125, 170
Bst6I CTCTTC 1 cut(s) 426
BstAFI CTTAAG 1 cut(s) 631
BstBAI YACGTR 1 cut(s) 664
BstBI TTCGAA 1 cut(s) 199
BstC8I GCNNGC 3 cut(s) 54, 194, 265
BstDEI CTNAG 3 cut(s) 531, 574, 597
BstEII GGTNACC 1 cut(s) 578
BstF5I GGATG 1 cut(s) 388
BstFNI CGCG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 2 cut(s) 591, 694
BstMAI GTCTC 5 cut(s) 106, 507, 534, 563, 643
BstMBI GATC 2 cut(s) 588, 691
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 578
BstSFI CTRYAG 1 cut(s) 481
BstSNI TACGTA 1 cut(s) 664
BstUI CGCG 1 cut(s) 552
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 2 cut(s) 588, 691
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 2 cut(s) 588, 691
BsuRI GGCC 1 cut(s) 134
BtgZI GCGATG 1 cut(s) 621
BtrI CACGTC 1 cut(s) 101
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 3 cut(s) 68, 164, 594
BtuMI TCGCGA 1 cut(s) 552
Cac8I GCNNGC 3 cut(s) 54, 194, 265
CaiI CAGNNNCTG 1 cut(s) 695
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 640
Csp6I GTAC 1 cut(s) 661
CviAII CATG 5 cut(s) 241, 268, 448, 537, 719
CviJI RGCY 4 cut(s) 134, 192, 263, 365
CviKI_1 RGCY 4 cut(s) 134, 192, 263, 365
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 3 cut(s) 531, 574, 597
DpnI GATC 2 cut(s) 590, 693
DpnII GATC 2 cut(s) 588, 691
DraIII CACNNNGTG 1 cut(s) 495
DrdI GACNNNNNNGTC 1 cut(s) 241
DriI GACNNNNNGTC 1 cut(s) 168
DseDI GACNNNNNNGTC 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 426
Eam1105I GACNNNNNGTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 426
Eco105I TACGTA 1 cut(s) 664
Eco31I GGTCTC 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 640
Eco47III AGCGCT 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 89
Eco91I GGTNACC 1 cut(s) 578
EcoO65I GGTNACC 1 cut(s) 578
EcoRI GAATTC 1 cut(s) 201
FaeI CATG 5 cut(s) 244, 271, 451, 540, 722
FaqI GGGAC 1 cut(s) 32
FatI CATG 5 cut(s) 240, 267, 447, 536, 718
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 152
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 132
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 1 cut(s) 134
HapII CCGG 3 cut(s) 128, 180, 724
HhaI GCGC 1 cut(s) 34
Hin1II CATG 5 cut(s) 244, 271, 451, 540, 722
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HinfI GANTC 5 cut(s) 20, 321, 386, 567, 713
HpaII CCGG 3 cut(s) 128, 180, 724
HphI GGTGA 3 cut(s) 164, 466, 590
Hpy166II GTNNAC 2 cut(s) 162, 250
Hpy188I TCNGA 4 cut(s) 81, 108, 391, 409
Hpy188III TCNNGA 4 cut(s) 224, 512, 551, 614
Hpy8I GTNNAC 2 cut(s) 162, 250
HpyAV CCTTC 2 cut(s) 306, 372
HpyCH4III ACNGT 4 cut(s) 12, 117, 125, 170
HpyCH4IV ACGT 2 cut(s) 100, 663
HpyCH4V TGCA 4 cut(s) 307, 360, 447, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 3 cut(s) 531, 574, 597
HpySE526I ACGT 2 cut(s) 100, 663
Hsp92II CATG 5 cut(s) 244, 271, 451, 540, 722
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 2 cut(s) 588, 691
LmnI GCTCC 2 cut(s) 95, 182
LweI GCATC 1 cut(s) 598
MaeI CTAG 1 cut(s) 152
MaeII ACGT 2 cut(s) 100, 663
MaeIII GTNAC 3 cut(s) 170, 485, 578
MalI GATC 2 cut(s) 590, 693
MboI GATC 2 cut(s) 588, 691
MboII GAAGA 3 cut(s) 95, 440, 443
MflI RGATCY 2 cut(s) 588, 691
MhlI GDGCHC 2 cut(s) 100, 187
MluCI AATT 3 cut(s) 201, 411, 441
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 9 cut(s) 75, 155, 185, 328, 333, 387, 445, 631, 687
MseI TTAA 2 cut(s) 27, 632
MslI CAYNNNNRTG 2 cut(s) 329, 452
MspCI CTTAAG 1 cut(s) 631
MspI CCGG 3 cut(s) 128, 180, 724
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 2 cut(s) 588, 691
NlaIII CATG 5 cut(s) 244, 271, 451, 540, 722
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 3 cut(s) 170, 485, 578
NruI TCGCGA 1 cut(s) 552
NspV TTCGAA 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 517
PfeI GAWTC 4 cut(s) 321, 386, 567, 713
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Ppu21I YACGTR 1 cut(s) 664
PspEI GGTNACC 1 cut(s) 578
PspPI GGNCC 1 cut(s) 640
PstI CTGCAG 1 cut(s) 485
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 2 cut(s) 588, 691
RruI TCGCGA 1 cut(s) 552
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
RseI CAYNNNNRTG 2 cut(s) 329, 452
SaqAI TTAA 2 cut(s) 27, 632
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 2 cut(s) 588, 691
Sau96I GGNCC 1 cut(s) 640
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 2 cut(s) 100, 187
SetI ASST 7 cut(s) 103, 177, 456, 530, 580, 585, 666
SfaNI GCATC 1 cut(s) 598
SfcI CTRYAG 1 cut(s) 481
SfuI TTCGAA 1 cut(s) 199
SinI GGWCC 1 cut(s) 640
SmiMI CAYNNNNRTG 2 cut(s) 329, 452
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
SnaBI TACGTA 1 cut(s) 664
Sse9I AATT 3 cut(s) 201, 411, 441
SsiI CCGC 2 cut(s) 131, 643
SspMI CTAG 1 cut(s) 152
TaaI ACNGT 4 cut(s) 12, 117, 125, 170
TaiI ACGT 2 cut(s) 103, 666
TaqI TCGA 3 cut(s) 199, 511, 615
TasI AATT 3 cut(s) 201, 411, 441
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 321, 386, 567, 713
Tru1I TTAA 2 cut(s) 27, 632
Tru9I TTAA 2 cut(s) 27, 632
TscAI CASTG 3 cut(s) 75, 164, 601
TseFI GTSAC 3 cut(s) 170, 485, 578
Tsp45I GTSAC 3 cut(s) 170, 485, 578
TspDTI ATGAA 1 cut(s) 399
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 3 cut(s) 75, 164, 601
Vha464I CTTAAG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 640
XapI RAATTY 2 cut(s) 201, 441
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.