pycom11g12650

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007314
Physical Location & Seq
Forward (+)
11533536 .. 11546010
12475 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3063 bp
ATGGCGTTGTCAGGCATGCGAGGCCTCTCTGTTTTCATCAGCGACGTTCGGAATTGCCAGAACAAAGAGCAGGAGAGGCTCCGGGTCGATAAGGAGCTTGGGAATGTCCGTACACGCTTCAAAAACGAAAAGGGTTTAACACCTTATGAAAAGAAGAAATATGTCTGGAAAATGCTTTACATTTATATGCTGGGCTACGATGTGGATTTTGGGCACATGGAAGCAGTTTCTCTAATATCTGCGCCTAAGTATCCAGAAAAGCAGGTTGGGTACATTGTAACAGCATGCTTGCTCAATGAGAACCATGATTTTCTGAGATTAGCGATAAATACCGTGCGCAATGACATCATTGGTCGGAATGAGACTTTCCAATGTTTGGCATTGACTATGGTTGGGAATATTGGTGGGCGAGAATTTGCGGAGTCATTAGCACCTGATGTTCAAAAGTTACTTATTTCTAGCAGCTACAGACCACTTGTGAGAAAAAAAGCTGCACTATGTCTATTGCGCTTGTATAGAAAAAATCCTGATGTTGTCAATGTAGATGGCTGGGCTGACCGGATGGCACAACTTTTAGATGAACGTGATCTTGGTGTTTTAACATCATCTATGAGCCTTCTTGTTGCCCTTGTATCAAACCACTATGATGCTTACTGGAGTTCTCTTCCAAAATGTGTTAAAATATTAGAACGACTAGCTAGGAACCAGGATATTCCACAAGAGTACACCTACTATGGGATCCCATCTCCTTGGCTTCAGGTCAAGACAATGAGGGCTCTTCAGTATTTTCCGACCGTCGAAGATCCAAATACCAGAAGATCATTATTTGAGGTCTTACAGCGGATTTTGATGGGTACTGATGTTGTGAAAAATGTTAATAAGAACAATGCATCACATGCTGTTCTCTTTGAAGCCCTCGCTCTAGTCATGCATCTTGATGCTGAAAAGGAGATGATGTCTCAGTGTGTTGCCCTTCTTGGAAAATTTATTGCTGTCCGAGAACCAAATATCCGATATCTTGGTTTGGAAAATATGACCCGCATGTTAATGGTTACTGATGTGCAAGATATCATCAAGAGACATCAGGCTCAGATCATCACCTCATTGAAGGATCCAGACATCAGTATACGTAGGCGTGCTCTTGACTTGCTTTATGGTATGTGTGATGTTTCAAATGCAAAGGATATAGTCGAAGAATTATTGCAGTATCTTAGCACAGCCGATTTTGCCATGCGTGAAGAATTGTCTCTTAAGGCTGCCATTCTTGCAGAAAAGTTTGCCCCCGATCTTTCTTGGTATGTAGATGTGATTCTTCAATTAATTGACAAGGCTGGAGATTTTGTTAGTGACGATATATGGTTTCGTGTTGTGCAATTTGTTACAAATAACGAAGACTTACAGCCTTATGCAGCAGCAAAAGCTAGGGAGTATCTTGACAAACCTGCTATACATGAGACTATGGTTAAGGTCAGTGCATATATCATCGGAGAATTTGGCCACCTTTTGGCCAGACGCCCTGGGTGCAGTCCAAAGGAATTATTTACCGTCATACATGAGAAGCTTCCAGCTGTAACGACATCTACTATACCTATTCTTCTTTCAACATATGCTAAGATCTTTATGCACACTCAACCTCCAGACCAGGAACTACAGAATCAGATCTGGTCAATATTCAACAAATATGAGAGTTGCATTGATGTTGAGATACAACAAAGAGCTGCTGAATACCTTGCCTTAAGTAGGAGAGGTGCAGATCTGGTGTATATTTTGGCTGAAATGCCTAAATTTCCTGAGCGGCAGTCTGCATTGATCAAGAAGGCTGAAGATACAGAGGTTGATACAGCAGAACAAAGTGCTATCAAGTTGCGGGCCCAGCAGCAAACATCAAGTGCTTTGGTTGTGACCGACCAACGCCCTGCTAACGGGACCTCGCCTGTCAACCAGCTCGGTCTTGTCAAGATGCCCAGCATGAGCAGTAACGCGGATCACAATTCAACTGACCAAGGGTCGTCTCAGGAAAATGGTACTTTGAGTAAAGTTGATCCTCAGCCACCTTCAGCAGATATCCTTGGTGATCTCTTGGGTCCCCTGGCTATTGAAGGCCGTCCTGCTACTGCTGGTCAGCCGCAGCAGACTGTAACCCCTGGATTAGGAAGCGATCCAAATGCAGTGGATGCTTCAGCAATTGTGCCTGTTGGCGAGGAGCAAAATTCTGTTCAGCCAATAGGAAATATTGCTGAAAGATTTCATGCTCTCTGCTTGAAAGATAGTGGTGTTCTATACGAAGATCCTAATATTCAGATTGGAGTTAAATCCGAGTGGCGGCTACATCAAGGATTTCTTGTTCTTTTCTTGGGAAACAAAAATACTTCACCACTTGTGTCAGTTCAGGCTATAATATTGCCTCCCTCTCATTTGAAAATGGAGCTCTCACTAGTACCGGATACTATTCCTCCCAGGGCACAGGTACAATGCCCGCTTGAAGTTGTGAATCTCAGACCTAGCAGGGATGTAGCGGTTCTTGACTTTTCCTACAAGTTTGGTAACAATATGGTCAATGTCAAACTTCGCCTCCCAGCTGTCTTAAGTAAATTTCTTCAGCCTATTCCGGTGTCTACTGAAGAGTTCTTTCCCCAGTGGAGATCACTATCAGGGCCACCTCTGAAGCTTCAAGAAGTGGTTAGAGGTGTCAAACCAATGCCACTTGTAGAAATGGCGAATCTACTTAACAGTTTCCGGTTGATGGTTTGTCCAGCGCTTGATCCAAATCCAAATAATCTTGTTGCAAGTACAACATTTTACTCAGAAAATACACGGGCCATGCTTTGCTTGGTTAGAATAGAAACAGATCCAGCAGACAGAACACAGTTGCGTATGACAGTTGCTTCAGGGGACCCTACATTAACGCTTGAGTTGAAGGAGTTCATCAAGGAACAATTAGTTTTCATGCCTCCAGCCCCTCATGCACCTGGACCAGTACCGCCAGCACCTCCAGTAGCTCAACCAACCAGCCCGGTTGCAGCATTAACAGATCCCGGGGCTATGCTGGCCGGCCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1021

Amino Acids

113.74

Weight (kDa)

5.96

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 28 - 582 2.2e-114 Adaptin N terminal region
Cnd1 PF12717 125 - 265 3.8e-07 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 757 - 853 1.8e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 865 - 974 1.3e-15 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 338
Acc36I ACCTGC 2 cut(s) 253, 1450
AccB7I CCANNNNNTGG 2 cut(s) 376, 1504
AccBSI CCGCTC 1 cut(s) 1795
AccI GTMKAC 2 cut(s) 1126, 2615
AccII CGCG 1 cut(s) 1982
AcoI YGGCCR 3 cut(s) 1495, 1506, 3048
AcsI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2209, 2591
AcuI CTGAAG 9 cut(s) 740, 764, 1842, 2040, 2163, 2582, 2640, 2684, 2871
AcyI GRCGYC 1 cut(s) 1513
AfaI GTAC 9 cut(s) 112, 272, 725, 856, 2026, 2439, 2469, 2791, 2979
AfeI AGCGCT 1 cut(s) 2757
AfiI CCNNNNNNNGG 8 cut(s) 376, 735, 1504, 1740, 1922, 2150, 2322, 2743
AflII CTTAAG 3 cut(s) 1250, 1735, 2584
AhdI GACNNNNNGTC 1 cut(s) 2005
AhlI ACTAGT 1 cut(s) 2434
AjnI CCWGG 7 cut(s) 705, 1516, 1641, 2088, 2143, 2456, 2968
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 573, 605
AloI GAACNNNNNNTCC 4 cut(s) 993, 1025, 2328, 2360
Alw21I GWGCWC 2 cut(s) 1141, 2430
Alw26I GTCTC 6 cut(s) 356, 963, 1072, 1251, 1448, 2016
Ama87I CYCGRG 1 cut(s) 3035
Aor51HI AGCGCT 1 cut(s) 2757
AoxI GGCC 9 cut(s) 22, 1495, 1506, 1869, 2101, 2654, 2817, 3048, 3052
ApaI GGGCCC 1 cut(s) 1873
ApeKI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 2128, 3020
ApoI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2209, 2591
AseI ATTAAT 1 cut(s) 1319
Asp700I GAANNNNTTC 2 cut(s) 2244, 2921
AspLEI GCGC 4 cut(s) 244, 339, 510, 2758
AspS9I GGNCC 8 cut(s) 1869, 1870, 1926, 2084, 2654, 2817, 2893, 2972
AsuC2I CCSGG 4 cut(s) 83, 3014, 3036, 3037
AsuHPI GGTGA 3 cut(s) 1090, 2084, 2364
AvaI CYCGRG 1 cut(s) 3035
AvaII GGWCC 4 cut(s) 1926, 2084, 2893, 2972
BaeGI GKGCMC 3 cut(s) 216, 1873, 2464
BaeI ACNNNNGTAYC 4 cut(s) 846, 879, 1818, 1851
BalI TGGCCA 2 cut(s) 1497, 1508
BamHI GGATCC 2 cut(s) 738, 1111
BanII GRGCYC 3 cut(s) 778, 1873, 2430
BbsI GAAGAC 1 cut(s) 1398
Bbv12I GWGCWC 2 cut(s) 1141, 2430
BbvCI CCTCAGC 1 cut(s) 2046
BbvI GCAGC 9 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1888, 2140, 3032
BccI CCATC 5 cut(s) 539, 556, 751, 844, 2737
BceAI ACGGC 1 cut(s) 2088
BcgI CGANNNNNNTGC 2 cut(s) 2147, 2181
BciT130I CCWGG 7 cut(s) 707, 1518, 1643, 2090, 2145, 2458, 2970
BciVI GTATCC 2 cut(s) 261, 2437
BclI TGATCA 1 cut(s) 1809
BcnI CCSGG 4 cut(s) 83, 3014, 3036, 3037
BcoDI GTCTC 6 cut(s) 356, 963, 1072, 1251, 1448, 2016
BcuI ACTAGT 1 cut(s) 2434
BfaI CTAG 7 cut(s) 459, 695, 699, 923, 1422, 2435, 2502
BfmI CTRYAG 2 cut(s) 466, 1649
BfoI RGCGCY 1 cut(s) 2759
BfrI CTTAAG 3 cut(s) 1250, 1735, 2584
BfuAI ACCTGC 2 cut(s) 253, 1450
BfuI GTATCC 2 cut(s) 261, 2437
BglII AGATCT 3 cut(s) 1614, 1659, 1753
Bme18I GGWCC 4 cut(s) 1926, 2084, 2893, 2972
BmeRI GACNNNNNGTC 1 cut(s) 2005
BmeT110I CYCGRG 1 cut(s) 3035
BmgT120I GGNCC 8 cut(s) 1869, 1870, 1926, 2084, 2654, 2817, 2893, 2972
BmrI ACTGGG 1 cut(s) 2629
BmsI GCATC 6 cut(s) 637, 899, 928, 940, 1950, 2164
BmuI ACTGGG 1 cut(s) 2629
BpiI GAAGAC 1 cut(s) 1398
BpmI CTGGAG 5 cut(s) 676, 1353, 1620, 2937, 2976
Bpu10I CCTNAGC 2 cut(s) 1791, 2046
BpuEI CTTGAG 1 cut(s) 2930
BpuMI CCSGG 4 cut(s) 83, 3014, 3036, 3037
BsaAI YACGTR 1 cut(s) 1130
BsaBI GATNNNNATC 2 cut(s) 2647, 2766
BsaHI GRCGYC 1 cut(s) 1513
BsaWI WCCGGW 4 cut(s) 558, 2440, 2608, 2736
Bsc4I CCNNNNNNNGG 8 cut(s) 376, 735, 1504, 1740, 1922, 2150, 2322, 2743
Bse118I RCCGGY 1 cut(s) 3050
Bse1I ACTGG 4 cut(s) 659, 2635, 2975, 2993
Bse3DI GCAATG 1 cut(s) 346
Bse8I GATNNNNATC 2 cut(s) 2647, 2766
BseBI CCWGG 7 cut(s) 707, 1518, 1643, 2090, 2145, 2458, 2970
BseGI GGATG 3 cut(s) 567, 2179, 2515
BseJI GATNNNNATC 2 cut(s) 2647, 2766
BseLI CCNNNNNNNGG 8 cut(s) 376, 735, 1504, 1740, 1922, 2150, 2322, 2743
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 8 cut(s) 305, 974, 1103, 1782, 2027, 2060, 2509, 2817
BseNI ACTGG 4 cut(s) 659, 2635, 2975, 2993
BseRI GAGGAG 1 cut(s) 2216
BseSI GKGCMC 3 cut(s) 216, 1873, 2464
BseXI GCAGC 9 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1888, 2140, 3032
BseYI CCCAGC 5 cut(s) 190, 549, 1872, 1964, 2575
BsgI GTGCAG 3 cut(s) 477, 1543, 1770
Bsh1236I CGCG 1 cut(s) 1982
Bsh1285I CGRYCG 1 cut(s) 795
BshFI GGCC 9 cut(s) 24, 1497, 1508, 1871, 2103, 2656, 2819, 3050, 3054
BsiEI CGRYCG 1 cut(s) 795
BsiHKAI GWGCWC 2 cut(s) 1141, 2430
BsiHKCI CYCGRG 1 cut(s) 3035
BsiSI CCGG 8 cut(s) 82, 559, 2441, 2609, 2737, 3014, 3036, 3051
BslFI GGGAC 3 cut(s) 1939, 2070, 2906
BslI CCNNNNNNNGG 8 cut(s) 376, 735, 1504, 1740, 1922, 2150, 2322, 2743
BsmAI GTCTC 6 cut(s) 356, 963, 1072, 1251, 1448, 2016
BsmBI CGTCTC 1 cut(s) 2016
BsmFI GGGAC 3 cut(s) 1939, 2070, 2906
BsnI GGCC 9 cut(s) 24, 1497, 1508, 1871, 2103, 2656, 2819, 3050, 3054
BsoBI CYCGRG 1 cut(s) 3035
Bsp120I GGGCCC 1 cut(s) 1869
Bsp1286I GDGCHC 6 cut(s) 216, 778, 1141, 1873, 2430, 2464
BspANI GGCC 9 cut(s) 24, 1497, 1508, 1871, 2103, 2656, 2819, 3050, 3054
BspCNI CTCAG 8 cut(s) 306, 973, 1102, 1783, 2026, 2059, 2508, 2816
BspFNI CGCG 1 cut(s) 1982
BspMI ACCTGC 2 cut(s) 253, 1450
BspQI GCTCTTC 1 cut(s) 783
BspTI CTTAAG 3 cut(s) 1250, 1735, 2584
BsrBI CCGCTC 1 cut(s) 1795
BsrDI GCAATG 1 cut(s) 346
BsrFI RCCGGY 1 cut(s) 3050
BsrI ACTGG 4 cut(s) 659, 2635, 2975, 2993
BssAI RCCGGY 1 cut(s) 3050
BssNAI GTATAC 1 cut(s) 1127
BssNI GRCGYC 1 cut(s) 1513
BssT1I CCWWGG 3 cut(s) 749, 2002, 2068
Bst1107I GTATAC 1 cut(s) 1127
Bst2UI CCWGG 7 cut(s) 707, 1518, 1643, 2090, 2145, 2458, 2970
Bst4CI ACNGT 7 cut(s) 334, 796, 1546, 2137, 2732, 2868, 2881
Bst6I CTCTTC 3 cut(s) 669, 783, 2616
BstACI GRCGYC 1 cut(s) 1513
BstAFI CTTAAG 3 cut(s) 1250, 1735, 2584
BstAPI GCANNNNNTGC 2 cut(s) 896, 2174
BstBAI YACGTR 1 cut(s) 1130
BstENI CCTNNNNNAGG 2 cut(s) 1738, 2148
BstF5I GGATG 3 cut(s) 567, 2179, 2515
BstFNI CGCG 1 cut(s) 1982
BstH2I RGCGCY 1 cut(s) 2759
BstHHI GCGC 4 cut(s) 244, 339, 510, 2758
BstMAI GTCTC 6 cut(s) 356, 963, 1072, 1251, 1448, 2016
BstMCI CGRYCG 1 cut(s) 795
BstNI CCWGG 7 cut(s) 707, 1518, 1643, 2090, 2145, 2458, 2970
BstNSI RCATGY 4 cut(s) 19, 288, 899, 1045
BstSFI CTRYAG 2 cut(s) 466, 1649
BstSLI GKGCMC 3 cut(s) 216, 1873, 2464
BstSNI TACGTA 1 cut(s) 1130
BstUI CGCG 1 cut(s) 1982
BstV1I GCAGC 9 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1888, 2140, 3032
BstV2I GAAGAC 1 cut(s) 1398
BstX2I RGATCY 9 cut(s) 738, 802, 1111, 1614, 1659, 1753, 2287, 2848, 3031
BstXI CCANNNNNNTGG 1 cut(s) 750
BstYI RGATCY 9 cut(s) 738, 802, 1111, 1614, 1659, 1753, 2287, 2848, 3031
BstZ17I GTATAC 1 cut(s) 1127
BsuI GTATCC 2 cut(s) 261, 2437
BsuRI GGCC 9 cut(s) 24, 1497, 1508, 1871, 2103, 2656, 2819, 3050, 3054
BtsCI GGATG 3 cut(s) 567, 2179, 2515
BtsI GCAGTG 1 cut(s) 2175
BtsIMutI CAGTG 4 cut(s) 968, 1477, 2175, 2642
BveI ACCTGC 2 cut(s) 253, 1450
CfoI GCGC 4 cut(s) 244, 339, 510, 2758
Cfr10I RCCGGY 1 cut(s) 3050
Cfr13I GGNCC 8 cut(s) 1869, 1870, 1926, 2084, 2654, 2817, 2893, 2972
Cfr9I CCCGGG 1 cut(s) 3035
CseI GACGC 1 cut(s) 1521
Csp6I GTAC 9 cut(s) 111, 271, 724, 855, 2025, 2438, 2468, 2790, 2978
CspCI CAANNNNNGTGG 2 cut(s) 2151, 2186
CviQI GTAC 9 cut(s) 111, 271, 724, 855, 2025, 2438, 2468, 2790, 2978
DriI GACNNNNNGTC 1 cut(s) 2005
EaeI YGGCCR 3 cut(s) 1495, 1506, 3048
Eam1104I CTCTTC 3 cut(s) 669, 783, 2616
Eam1105I GACNNNNNGTC 1 cut(s) 2005
EarI CTCTTC 3 cut(s) 669, 783, 2616
Ecl136II GAGCTC 1 cut(s) 2428
Eco105I TACGTA 1 cut(s) 1130
Eco130I CCWWGG 3 cut(s) 749, 2002, 2068
Eco147I AGGCCT 1 cut(s) 24
Eco24I GRGCYC 3 cut(s) 778, 1873, 2430
Eco32I GATATC 3 cut(s) 1016, 1069, 2065
Eco47I GGWCC 4 cut(s) 1926, 2084, 2893, 2972
Eco47III AGCGCT 1 cut(s) 2757
Eco53kI GAGCTC 1 cut(s) 2428
Eco57I CTGAAG 9 cut(s) 740, 764, 1842, 2040, 2163, 2582, 2640, 2684, 2871
Eco88I CYCGRG 1 cut(s) 3035
EcoICRI GAGCTC 1 cut(s) 2428
EcoNI CCTNNNNNAGG 2 cut(s) 1738, 2148
EcoO109I RGGNCCY 3 cut(s) 1926, 2084, 2893
EcoRII CCWGG 7 cut(s) 705, 1516, 1641, 2088, 2143, 2456, 2968
EcoRV GATATC 3 cut(s) 1016, 1069, 2065
EcoT14I CCWWGG 3 cut(s) 749, 2002, 2068
EcoT22I ATGCAT 2 cut(s) 892, 933
EcoT38I GRGCYC 3 cut(s) 778, 1873, 2430
ErhI CCWWGG 3 cut(s) 749, 2002, 2068
Esp3I CGTCTC 1 cut(s) 2016
FalI AAGNNNNNCTT 2 cut(s) 2325, 2357
FaqI GGGAC 3 cut(s) 1939, 2070, 2906
FauI CCCGC 3 cut(s) 1046, 1860, 2484
FauNDI CATATG 1 cut(s) 1606
FbaI TGATCA 1 cut(s) 1809
FblI GTMKAC 2 cut(s) 1126, 2615
FokI GGATG 3 cut(s) 574, 2186, 2522
FriOI GRGCYC 3 cut(s) 778, 1873, 2430
FseI GGCCGGCC 1 cut(s) 3054
FspBI CTAG 7 cut(s) 459, 695, 699, 923, 1422, 2435, 2502
FspI TGCGCA 1 cut(s) 338
GlaI GCGC 4 cut(s) 243, 338, 509, 2757
GsaI CCCAGC 5 cut(s) 194, 553, 1876, 1968, 2579
GsuI CTGGAG 5 cut(s) 676, 1353, 1620, 2937, 2976
HaeII RGCGCY 1 cut(s) 2759
HaeIII GGCC 9 cut(s) 24, 1497, 1508, 1871, 2103, 2656, 2819, 3050, 3054
HapII CCGG 8 cut(s) 82, 559, 2441, 2609, 2737, 3014, 3036, 3051
HgaI GACGC 1 cut(s) 1521
HhaI GCGC 4 cut(s) 244, 339, 510, 2758
Hin1I GRCGYC 1 cut(s) 1513
Hin6I GCGC 4 cut(s) 242, 337, 508, 2756
HinP1I GCGC 4 cut(s) 242, 337, 508, 2756
HincII GTYRAC 1 cut(s) 1939
HindII GTYRAC 1 cut(s) 1939
HindIII AAGCTT 2 cut(s) 1559, 2666
HinfI GANTC 5 cut(s) 422, 1309, 1654, 2491, 2719
HpaII CCGG 8 cut(s) 82, 559, 2441, 2609, 2737, 3014, 3036, 3051
HphI GGTGA 3 cut(s) 1090, 2084, 2364
Hpy166II GTNNAC 5 cut(s) 113, 726, 1127, 1939, 2616
Hpy8I GTNNAC 5 cut(s) 113, 726, 1127, 1939, 2616
Hpy99I CGWCG 2 cut(s) 47, 800
HpyAV CCTTC 7 cut(s) 626, 983, 1102, 1810, 2064, 2093, 2911
HpyCH4III ACNGT 7 cut(s) 334, 796, 1546, 2137, 2732, 2868, 2881
HpyCH4IV ACGT 3 cut(s) 45, 583, 1129
HpySE526I ACGT 3 cut(s) 45, 583, 1129
Hsp92I GRCGYC 1 cut(s) 1513
HspAI GCGC 4 cut(s) 242, 337, 508, 2756
KflI GGGWCCC 2 cut(s) 2084, 2893
KroI GCCGGC 1 cut(s) 3050
KroNI GCCGGC 1 cut(s) 3052
Ksp22I TGATCA 1 cut(s) 1809
LguI GCTCTTC 1 cut(s) 783
LmnI GCTCC 4 cut(s) 84, 94, 2203, 2425
Lsp1109I GCAGC 9 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1888, 2140, 3032
LweI GCATC 6 cut(s) 637, 899, 928, 940, 1950, 2164
MaeI CTAG 7 cut(s) 459, 695, 699, 923, 1422, 2435, 2502
MaeII ACGT 3 cut(s) 45, 583, 1129
MbiI CCGCTC 1 cut(s) 1795
MfeI CAATTG 1 cut(s) 2184
MflI RGATCY 9 cut(s) 738, 802, 1111, 1614, 1659, 1753, 2287, 2848, 3031
MhlI GDGCHC 6 cut(s) 216, 778, 1141, 1873, 2430, 2464
MlsI TGGCCA 2 cut(s) 1497, 1508
MluNI TGGCCA 2 cut(s) 1497, 1508
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 2 cut(s) 335, 815
Mox20I TGGCCA 2 cut(s) 1497, 1508
Mph1103I ATGCAT 2 cut(s) 892, 933
MroNI GCCGGC 1 cut(s) 3050
MroXI GAANNNNTTC 2 cut(s) 2244, 2921
MscI TGGCCA 2 cut(s) 1497, 1508
MslI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
Msp20I TGGCCA 2 cut(s) 1497, 1508
MspA1I CMGCKG 3 cut(s) 841, 1568, 2579
MspCI CTTAAG 3 cut(s) 1250, 1735, 2584
MspI CCGG 8 cut(s) 82, 559, 2441, 2609, 2737, 3014, 3036, 3051
MunI CAATTG 1 cut(s) 2184
MvaI CCWGG 7 cut(s) 707, 1518, 1643, 2090, 2145, 2458, 2970
MvnI CGCG 1 cut(s) 1982
NaeI GCCGGC 1 cut(s) 3052
NciI CCSGG 4 cut(s) 83, 3014, 3036, 3037
NdeI CATATG 1 cut(s) 1606
NgoMIV GCCGGC 1 cut(s) 3050
NmuCI GTSAC 2 cut(s) 1346, 1900
NsbI TGCGCA 1 cut(s) 338
NsiI ATGCAT 2 cut(s) 892, 933
NspI RCATGY 4 cut(s) 19, 288, 899, 1045
PaeI GCATGC 2 cut(s) 19, 288
PasI CCCWGGG 2 cut(s) 1517, 2457
PceI AGGCCT 1 cut(s) 24
PciSI GCTCTTC 1 cut(s) 783
PdiI GCCGGC 1 cut(s) 3052
PdmI GAANNNNTTC 2 cut(s) 2244, 2921
PfeI GAWTC 4 cut(s) 1309, 1654, 2491, 2719
PflMI CCANNNNNTGG 2 cut(s) 376, 1504
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 1130
PpuMI RGGWCCY 3 cut(s) 1926, 2084, 2893
PshBI ATTAAT 1 cut(s) 1319
Psp124BI GAGCTC 1 cut(s) 2430
Psp5II RGGWCCY 3 cut(s) 1926, 2084, 2893
Psp6I CCWGG 7 cut(s) 705, 1516, 1641, 2088, 2143, 2456, 2968
PspFI CCCAGC 5 cut(s) 190, 549, 1872, 1964, 2575
PspGI CCWGG 7 cut(s) 705, 1516, 1641, 2088, 2143, 2456, 2968
PspOMI GGGCCC 1 cut(s) 1869
PspPI GGNCC 8 cut(s) 1869, 1870, 1926, 2084, 2654, 2817, 2893, 2972
PspPPI RGGWCCY 3 cut(s) 1926, 2084, 2893
PsuI RGATCY 9 cut(s) 738, 802, 1111, 1614, 1659, 1753, 2287, 2848, 3031
PvuII CAGCTG 2 cut(s) 1568, 2579
RigI GGCCGGCC 1 cut(s) 3054
RsaI GTAC 9 cut(s) 112, 272, 725, 856, 2026, 2439, 2469, 2791, 2979
RsaNI GTAC 9 cut(s) 111, 271, 724, 855, 2025, 2438, 2468, 2790, 2978
RseI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SacI GAGCTC 1 cut(s) 2430
SapI GCTCTTC 1 cut(s) 783
Sau96I GGNCC 8 cut(s) 1869, 1870, 1926, 2084, 2654, 2817, 2893, 2972
SchI GAGTC 1 cut(s) 431
SduI GDGCHC 6 cut(s) 216, 778, 1141, 1873, 2430, 2464
SfaNI GCATC 6 cut(s) 637, 899, 928, 940, 1950, 2164
SfcI CTRYAG 2 cut(s) 466, 1649
SinI GGWCC 4 cut(s) 1926, 2084, 2893, 2972
SmaI CCCGGG 1 cut(s) 3037
SmiMI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SmlI CTYRAG 4 cut(s) 1250, 1735, 2584, 2909
SmoI CTYRAG 4 cut(s) 1250, 1735, 2584, 2909
SnaBI TACGTA 1 cut(s) 1130
SpeI ACTAGT 1 cut(s) 2434
SphI GCATGC 2 cut(s) 19, 288
SseBI AGGCCT 1 cut(s) 24
SspI AATATT 6 cut(s) 400, 684, 1671, 2233, 2296, 2400
SspMI CTAG 7 cut(s) 459, 695, 699, 923, 1422, 2435, 2502
SstI GAGCTC 1 cut(s) 2430
StuI AGGCCT 1 cut(s) 24
StyI CCWWGG 3 cut(s) 749, 2002, 2068
TaaI ACNGT 7 cut(s) 334, 796, 1546, 2137, 2732, 2868, 2881
TaiI ACGT 3 cut(s) 48, 586, 1132
TaqI TCGA 3 cut(s) 87, 798, 1191
TaqII GACCGA 2 cut(s) 1919, 1937
TatI WGTACW 2 cut(s) 723, 2789
TauI GCSGC 3 cut(s) 1798, 2128, 2326
TfiI GAWTC 4 cut(s) 1309, 1654, 2491, 2719
TscAI CASTG 4 cut(s) 968, 1477, 2175, 2642
TseFI GTSAC 2 cut(s) 1346, 1900
TseI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 2128, 3020
Tsp45I GTSAC 2 cut(s) 1346, 1900
TspDTI ATGAA 6 cut(s) 25, 162, 594, 2237, 2914, 2935
TspGWI ACGGA 1 cut(s) 98
TspMI CCCGGG 1 cut(s) 3035
TspRI CASTG 4 cut(s) 968, 1477, 2175, 2642
Van91I CCANNNNNTGG 2 cut(s) 376, 1504
Vha464I CTTAAG 3 cut(s) 1250, 1735, 2584
VpaK11BI GGWCC 4 cut(s) 1926, 2084, 2893, 2972
VspI ATTAAT 1 cut(s) 1319
XagI CCTNNNNNAGG 2 cut(s) 1738, 2148
XapI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2209, 2591
XceI RCATGY 4 cut(s) 19, 288, 899, 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 2827
XmaI CCCGGG 1 cut(s) 3035
XmiI GTMKAC 2 cut(s) 1126, 2615
XmnI GAANNNNTTC 2 cut(s) 2244, 2921
XspI CTAG 7 cut(s) 459, 695, 699, 923, 1422, 2435, 2502
Zsp2I ATGCAT 2 cut(s) 892, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.