pycom11g13680

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011144
Physical Location & Seq
Reverse (-)
13260513 .. 13262661
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 852 bp
ATGGCTTCCAATGGGAAATACGGCTCTCACCCTTACGCGCCCTCTGCGCCATCCCTGCCCGAGTCCTACGGGCAGCAACACACCCAATACCCCCATGGTTACGGGCGGCCCCAACAGCAGCCCGAGTCCTACGGGCAGCAACACACCCAATACCCCCATGGTTACGGGCGGCCCCAACAGCAGCATAATTCGTCGTCTTCTTCTCCCCAGTACTCCCATGGTTACGGGCAGCCGCAGCATCAGCCATATGGGTCGTCTTTTTCTTCTTCTTCTTCTTCGTCTTCATATGGGCAGTCGGGTTTTCCCGCAGGGACCGACCCGGCCGTGATTAGAAGCTTTGAGATGGTGGATGGGGACAGGAGTGGGTACATCGACGAGCACGAGCTGCAGCAAGCTCTGTCTTCTGGGTACCAGAGGTTTAGCCTTAGGACCATTCGCCTGCTCATTTTTCTCTTCAAAAGTCCCACGGATTCTCTCAGAGTCGGGCCGAAGGAGTTTGCTGCACTCTGGATTTGTCTTGGACAATGGCGATCCATATTCGAGAGGTATGACAAAGATAGGAGTGGAAAGATTGATTCGATGGAACTGAGAGATGCACTCTACGGTCTAGGCCTTGCCATGCCACCTTCTGTTCTTCAACTTTTGATTTCCAAATATGACGATGGAAGCGGGCGCAGGGTTGAACTCAACTTTGACAGTTTCGTTGAATGCGGGATGATTGTGAGGGTAAGCAAATTGCTAGGTTTGACCGATAGGTTCAAAGAGAAAGATCGGCGGTATACTGGTTCGGCAACTTTTGGTTACGACGAATTTATGTCTATGGTCATCCCATTTCTTGTGTCGTATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

284

Amino Acids

31.8

Weight (kDa)

7.13

Isoelectric Point (pI)

58.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 175 - 204 8e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 408
AccB1I GGYRCC 1 cut(s) 408
AccI GTMKAC 1 cut(s) 779
AccII CGCG 1 cut(s) 38
AciI CCGC 7 cut(s) 106, 169, 233, 306, 669, 711, 775
AclWI GGATC 1 cut(s) 525
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 809
AfaI GTAC 3 cut(s) 212, 368, 410
AgsI TTSAA 5 cut(s) 457, 638, 683, 707, 760
AluBI AGCT 3 cut(s) 336, 385, 395
AluI AGCT 3 cut(s) 336, 385, 395
Alw21I GWGCWC 1 cut(s) 381
AlwI GGATC 1 cut(s) 525
Ama87I CYCGRG 2 cut(s) 59, 122
AoxI GGCC 5 cut(s) 107, 170, 321, 485, 610
ApeKI GCWGC 9 cut(s) 73, 118, 136, 181, 229, 235, 385, 388, 500
ApoI RAATTY 1 cut(s) 809
Asp718I GGTACC 1 cut(s) 408
AspLEI GCGC 3 cut(s) 40, 49, 675
AspS9I GGNCC 5 cut(s) 108, 171, 312, 429, 485
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 20
AvaI CYCGRG 2 cut(s) 59, 122
AvaII GGWCC 2 cut(s) 312, 429
AxyI CCTNAGG 1 cut(s) 425
BanI GGYRCC 1 cut(s) 408
BauI CACGAG 1 cut(s) 380
BbsI GAAGAC 3 cut(s) 189, 273, 393
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 9 cut(s) 85, 130, 148, 193, 241, 247, 372, 400, 487
BccI CCATC 5 cut(s) 58, 337, 344, 574, 656
BceAI ACGGC 2 cut(s) 37, 308
BcnI CCSGG 1 cut(s) 320
BfaI CTAG 2 cut(s) 608, 740
BfmI CTRYAG 1 cut(s) 386
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 2 cut(s) 312, 429
BmeT110I CYCGRG 2 cut(s) 59, 122
BmgT120I GGNCC 5 cut(s) 108, 171, 312, 429, 485
BmiI GGNNCC 4 cut(s) 110, 173, 313, 410
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 1 cut(s) 202
BmsI GCATC 2 cut(s) 247, 583
BmuI ACTGGG 1 cut(s) 202
BpiI GAAGAC 3 cut(s) 189, 273, 393
BplI GAGNNNNNCTC 2 cut(s) 582, 614
BpuMI CCSGG 1 cut(s) 320
BsaJI CCNNGG 4 cut(s) 94, 157, 217, 465
Bse1I ACTGG 2 cut(s) 208, 787
Bse21I CCTNAGG 1 cut(s) 425
BseDI CCNNGG 4 cut(s) 94, 157, 217, 465
BseGI GGATG 4 cut(s) 50, 355, 720, 825
BseMII CTCAG 2 cut(s) 490, 578
BseNI ACTGG 2 cut(s) 208, 787
BseX3I CGGCCG 1 cut(s) 321
BseXI GCAGC 9 cut(s) 85, 130, 148, 193, 241, 247, 372, 400, 487
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 5 cut(s) 109, 172, 323, 487, 612
BshNI GGYRCC 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 2 cut(s) 59, 122
BsiSI CCGG 1 cut(s) 320
BslFI GGGAC 3 cut(s) 325, 368, 447
BsmFI GGGAC 3 cut(s) 325, 368, 447
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 5 cut(s) 109, 172, 323, 487, 612
BsoBI CYCGRG 2 cut(s) 59, 122
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 530, 769
Bsp19I CCATGG 3 cut(s) 94, 157, 217
BspACI CCGC 7 cut(s) 106, 169, 233, 306, 669, 711, 775
BspANI GGCC 5 cut(s) 109, 172, 323, 487, 612
BspCNI CTCAG 2 cut(s) 489, 579
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 4 cut(s) 110, 173, 313, 410
BspMAI CTGCAG 1 cut(s) 390
BspPI GGATC 1 cut(s) 525
BspT107I GGYRCC 1 cut(s) 408
BsrI ACTGG 2 cut(s) 208, 787
BssECI CCNNGG 4 cut(s) 94, 157, 217, 465
BssMI GATC 2 cut(s) 530, 769
BssNAI GTATAC 1 cut(s) 780
BssSI CACGAG 1 cut(s) 380
BssT1I CCWWGG 3 cut(s) 94, 157, 217
Bst1107I GTATAC 1 cut(s) 780
Bst2BI CACGAG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 605, 698
Bst6I CTCTTC 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 385
BstC8I GCNNGC 3 cut(s) 393, 440, 671
BstDEI CTNAG 3 cut(s) 425, 476, 587
BstDSI CCRYGG 4 cut(s) 94, 157, 217, 465
BstF5I GGATG 4 cut(s) 50, 355, 720, 825
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 3 cut(s) 40, 49, 675
BstKTI GATC 2 cut(s) 533, 772
BstMBI GATC 2 cut(s) 530, 769
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 8 cut(s) 44, 46, 55, 115, 178, 235, 241, 385
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 386
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 9 cut(s) 85, 130, 148, 193, 241, 247, 372, 400, 487
BstV2I GAAGAC 3 cut(s) 189, 273, 393
BstZ17I GTATAC 1 cut(s) 780
BstZI CGGCCG 1 cut(s) 321
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 5 cut(s) 109, 172, 323, 487, 612
BtgI CCRYGG 4 cut(s) 94, 157, 217, 465
BtsCI GGATG 4 cut(s) 50, 355, 720, 825
Cac8I GCNNGC 3 cut(s) 393, 440, 671
CfoI GCGC 3 cut(s) 40, 49, 675
Cfr13I GGNCC 5 cut(s) 108, 171, 312, 429, 485
Csp6I GTAC 3 cut(s) 211, 367, 409
CviAII CATG 4 cut(s) 95, 158, 218, 619
CviQI GTAC 3 cut(s) 211, 367, 409
DdeI CTNAG 3 cut(s) 425, 476, 587
DpnI GATC 2 cut(s) 532, 771
DpnII GATC 2 cut(s) 530, 769
EaeI YGGCCR 1 cut(s) 321
EagI CGGCCG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
EclXI CGGCCG 1 cut(s) 321
Eco130I CCWWGG 3 cut(s) 94, 157, 217
Eco147I AGGCCT 1 cut(s) 612
Eco47I GGWCC 2 cut(s) 312, 429
Eco52I CGGCCG 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 425
Eco88I CYCGRG 2 cut(s) 59, 122
EcoT14I CCWWGG 3 cut(s) 94, 157, 217
ErhI CCWWGG 3 cut(s) 94, 157, 217
FaeI CATG 4 cut(s) 98, 161, 221, 622
FaqI GGGAC 3 cut(s) 325, 368, 447
FatI CATG 4 cut(s) 94, 157, 217, 618
FauI CCCGC 3 cut(s) 313, 662, 704
FauNDI CATATG 2 cut(s) 247, 286
FblI GTMKAC 1 cut(s) 779
FokI GGATG 4 cut(s) 37, 362, 727, 812
FspBI CTAG 2 cut(s) 608, 740
GlaI GCGC 3 cut(s) 39, 48, 674
HaeIII GGCC 5 cut(s) 109, 172, 323, 487, 612
HapII CCGG 1 cut(s) 320
HhaI GCGC 3 cut(s) 40, 49, 675
Hin1II CATG 4 cut(s) 98, 161, 221, 622
Hin6I GCGC 3 cut(s) 38, 47, 673
HinP1I GCGC 3 cut(s) 38, 47, 673
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 5 cut(s) 62, 125, 470, 480, 575
HpaII CCGG 1 cut(s) 320
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 1 cut(s) 780
Hpy188I TCNGA 1 cut(s) 479
Hpy188III TCNNGA 2 cut(s) 508, 541
Hpy8I GTNNAC 1 cut(s) 780
Hpy99I CGWCG 3 cut(s) 196, 377, 809
HpyAV CCTTC 2 cut(s) 484, 636
HpyCH4III ACNGT 2 cut(s) 605, 698
HpyCH4V TGCA 3 cut(s) 388, 503, 596
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 46, 55, 115, 178, 235, 241, 385
HpyF3I CTNAG 3 cut(s) 425, 476, 587
Hsp92II CATG 4 cut(s) 98, 161, 221, 622
HspAI GCGC 3 cut(s) 38, 47, 673
KpnI GGTACC 1 cut(s) 412
Kzo9I GATC 2 cut(s) 530, 769
Lsp1109I GCAGC 9 cut(s) 85, 130, 148, 193, 241, 247, 372, 400, 487
LweI GCATC 2 cut(s) 247, 583
MaeI CTAG 2 cut(s) 608, 740
MaeIII GTNAC 4 cut(s) 98, 161, 221, 800
MalI GATC 2 cut(s) 532, 771
MboI GATC 2 cut(s) 530, 769
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 4 cut(s) 187, 734, 809, 847
MlyI GAGTC 3 cut(s) 71, 134, 489
MnlI CCTC 4 cut(s) 52, 408, 537, 717
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 320
Mva1269I GAATGC 1 cut(s) 713
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 8 cut(s) 44, 46, 55, 115, 178, 235, 241, 385
NciI CCSGG 1 cut(s) 320
NcoI CCATGG 3 cut(s) 94, 157, 217
NdeI CATATG 2 cut(s) 247, 286
NdeII GATC 2 cut(s) 530, 769
NlaIII CATG 4 cut(s) 98, 161, 221, 622
NlaIV GGNNCC 4 cut(s) 110, 173, 313, 410
PceI AGGCCT 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PctI GAATGC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 470, 575
PleI GAGTC 3 cut(s) 70, 133, 488
PpsI GAGTC 3 cut(s) 70, 133, 488
PspN4I GGNNCC 4 cut(s) 110, 173, 313, 410
PspPI GGNCC 5 cut(s) 108, 171, 312, 429, 485
PstI CTGCAG 1 cut(s) 390
RsaI GTAC 3 cut(s) 212, 368, 410
RsaNI GTAC 3 cut(s) 211, 367, 409
Sau3AI GATC 2 cut(s) 530, 769
Sau96I GGNCC 5 cut(s) 108, 171, 312, 429, 485
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 3 cut(s) 71, 134, 489
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 1 cut(s) 381
SetI ASST 8 cut(s) 338, 387, 397, 419, 548, 628, 745, 758
SfaNI GCATC 2 cut(s) 247, 583
SfcI CTRYAG 1 cut(s) 386
SinI GGWCC 2 cut(s) 312, 429
Sse9I AATT 4 cut(s) 187, 734, 809, 847
SseBI AGGCCT 1 cut(s) 612
SsiI CCGC 7 cut(s) 106, 169, 233, 306, 669, 711, 775
SspMI CTAG 2 cut(s) 608, 740
StuI AGGCCT 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 3 cut(s) 94, 157, 217
TaaI ACNGT 2 cut(s) 605, 698
TaqI TCGA 3 cut(s) 372, 540, 578
TaqII GACCGA 2 cut(s) 329, 764
TasI AATT 4 cut(s) 187, 734, 809, 847
TatI WGTACW 1 cut(s) 210
TauI GCSGC 3 cut(s) 109, 172, 235
TfiI GAWTC 2 cut(s) 470, 575
TseI GCWGC 9 cut(s) 73, 118, 136, 181, 229, 235, 385, 388, 500
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 1 cut(s) 482
VpaK11BI GGWCC 2 cut(s) 312, 429
XapI RAATTY 1 cut(s) 809
XcmI CCANNNNNNNNNTGG 3 cut(s) 92, 155, 215
XmiI GTMKAC 1 cut(s) 779
XspI CTAG 2 cut(s) 608, 740
ZrmI AGTACT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.