pycom11g16110

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012390
Physical Location & Seq
Reverse (-)
17462558 .. 17463199
642 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGGCAATGCAGTTTCTCCCTGTTTCCAAAAAAACGCTAACTCAGATGTGAAAGTAATATTTTCAGATGGCAGCACAAGACTCCTTAAAGGAAAACACATCGCTGGAGAGATCATGTTTGAGTTTCCAGACCAAATGGTTTGCCACGCAGACTCTTTCTTCATCGGCCACCCCATTCCGGCTTTAGCCATCGACGACGAGCTCATGCATGGCCAGACCTATTTCGTTCTTCCGATTGATCGTTTCGCATGCAGCGTCCTCACAGCCTCGACTCTTGCTGCCCTAGGCTCATCGTCAAGCCCTAACAAGAAGGCAGCTCCTATCAATTTTGGGGAGTGCCCGTTTGAGTACATAAAGAGTTCGGATGGAAAGGTCTTGATCAAGGTGGTACCGGAGTTCATAACAAGGTTAATTGCAAGAGGTCACAAGAAAATAAATGGGTCGTCTGATGATGCTGCTAGTTCTGGTAGTAGTACTTTTCTTTGTAGCACACCTGAGCTGCAAAATCACTATGAACAGCTTGTTGTGTCTAAAGAGCAATTATGGTCGCCTAAGCTTGAAACTATTTCTGAATACAAGGTTAGGTTTTCACCATGTAAATATATAGGGAAGTGGAAACAAAAAGAGCAAGAACTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

214

Amino Acids

23.51

Weight (kDa)

6.99

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 191 3.9e-40 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 388
AccB1I GGYRCC 1 cut(s) 388
AcoI YGGCCR 2 cut(s) 166, 211
AfaI GTAC 3 cut(s) 350, 390, 475
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 1 cut(s) 560
AluBI AGCT 5 cut(s) 202, 317, 499, 520, 556
AluI AGCT 5 cut(s) 202, 317, 499, 520, 556
Alw21I GWGCWC 1 cut(s) 204
AoxI GGCC 2 cut(s) 166, 211
ApeKI GCWGC 6 cut(s) 72, 252, 278, 314, 455, 499
Asp718I GGTACC 1 cut(s) 388
AspA2I CCTAGG 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 582
AvrII CCTAGG 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 380, 413
BalI TGGCCA 1 cut(s) 213
BanI GGYRCC 1 cut(s) 388
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 6 cut(s) 84, 264, 265, 326, 442, 486
BccI CCATC 3 cut(s) 62, 197, 359
BclI TGATCA 1 cut(s) 378
BfaI CTAG 2 cut(s) 284, 459
BisI GCNGC 6 cut(s) 73, 253, 279, 315, 456, 500
BlnI CCTAGG 1 cut(s) 283
BlsI GCNGC 6 cut(s) 74, 254, 280, 316, 457, 501
BmcAI AGTACT 1 cut(s) 475
BmiI GGNNCC 1 cut(s) 390
BmsI GCATC 1 cut(s) 442
BpmI CTGGAG 1 cut(s) 126
Bpu10I CCTNAGC 2 cut(s) 495, 552
BsaJI CCNNGG 1 cut(s) 283
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 57, 486
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 6 cut(s) 84, 264, 265, 326, 442, 486
BshFI GGCC 2 cut(s) 168, 213
BshNI GGYRCC 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 2 cut(s) 179, 392
BslI CCNNNNNNNGG 1 cut(s) 178
BsnI GGCC 2 cut(s) 168, 213
Bsp1286I GDGCHC 2 cut(s) 204, 341
Bsp143I GATC 3 cut(s) 111, 238, 378
BspANI GGCC 2 cut(s) 168, 213
BspCNI CTCAG 2 cut(s) 56, 487
BspLI GGNNCC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 3 cut(s) 111, 238, 378
BssT1I CCWWGG 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 3 cut(s) 43, 495, 552
BstF5I GGATG 1 cut(s) 370
BstKTI GATC 3 cut(s) 114, 241, 381
BstMBI GATC 3 cut(s) 111, 238, 378
BstNSI RCATGY 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 341
BstV1I GCAGC 6 cut(s) 84, 264, 265, 326, 442, 486
BsuRI GGCC 2 cut(s) 168, 213
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 370
Cac8I GCNNGC 1 cut(s) 250
CseI GACGC 1 cut(s) 244
Csp6I GTAC 3 cut(s) 349, 389, 474
CviAII CATG 5 cut(s) 115, 205, 209, 249, 594
CviQI GTAC 3 cut(s) 349, 389, 474
DdeI CTNAG 3 cut(s) 43, 495, 552
DpnI GATC 3 cut(s) 113, 240, 380
DpnII GATC 3 cut(s) 111, 238, 378
EaeI YGGCCR 2 cut(s) 166, 211
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 204
Eco53kI GAGCTC 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 210
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 5 cut(s) 118, 208, 212, 252, 597
FatI CATG 5 cut(s) 114, 204, 208, 248, 593
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 6 cut(s) 73, 253, 279, 315, 456, 500
FokI GGATG 1 cut(s) 377
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 6 cut(s) 73, 253, 279, 315, 456, 500
FspBI CTAG 2 cut(s) 284, 459
GluI GCNGC 6 cut(s) 73, 253, 279, 315, 456, 500
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 2 cut(s) 168, 213
HapII CCGG 2 cut(s) 179, 392
HgaI GACGC 1 cut(s) 244
Hin1II CATG 5 cut(s) 118, 208, 212, 252, 597
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 3 cut(s) 81, 152, 271
HpaII CCGG 2 cut(s) 179, 392
HphI GGTGA 1 cut(s) 582
Hpy188I TCNGA 6 cut(s) 46, 67, 234, 364, 448, 571
Hpy188III TCNNGA 2 cut(s) 128, 376
Hpy99I CGWCG 2 cut(s) 197, 200
HpyAV CCTTC 1 cut(s) 304
HpyCH4V TGCA 5 cut(s) 11, 208, 252, 416, 502
HpyF3I CTNAG 3 cut(s) 43, 495, 552
Hsp92II CATG 5 cut(s) 118, 208, 212, 252, 597
KpnI GGTACC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 3 cut(s) 111, 238, 378
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 8 cut(s) 34, 90, 141, 192, 227, 405, 450, 507
Lsp1109I GCAGC 6 cut(s) 84, 264, 265, 326, 442, 486
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 284, 459
MaeIII GTNAC 1 cut(s) 422
MalI GATC 3 cut(s) 113, 240, 380
MboI GATC 3 cut(s) 111, 238, 378
MboII GAAGA 2 cut(s) 151, 221
MhlI GDGCHC 2 cut(s) 204, 341
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 3 cut(s) 325, 411, 539
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 3 cut(s) 75, 146, 265
MnlI CCTC 3 cut(s) 269, 277, 413
Mox20I TGGCCA 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 210
MscI TGGCCA 1 cut(s) 213
MseI TTAA 2 cut(s) 87, 410
Msp20I TGGCCA 1 cut(s) 213
MspI CCGG 2 cut(s) 179, 392
NdeII GATC 3 cut(s) 111, 238, 378
NlaIII CATG 5 cut(s) 118, 208, 212, 252, 597
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 210
NspI RCATGY 1 cut(s) 252
PaeI GCATGC 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PkrI GCNGC 6 cut(s) 74, 254, 280, 316, 457, 501
PleI GAGTC 3 cut(s) 75, 146, 265
PpsI GAGTC 3 cut(s) 75, 146, 265
Psp124BI GAGCTC 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 390
RsaI GTAC 3 cut(s) 350, 390, 475
RsaNI GTAC 3 cut(s) 349, 389, 474
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 87, 410
SatI GCNGC 6 cut(s) 73, 253, 279, 315, 456, 500
Sau3AI GATC 3 cut(s) 111, 238, 378
ScaI AGTACT 1 cut(s) 475
SchI GAGTC 3 cut(s) 75, 146, 265
SduI GDGCHC 2 cut(s) 204, 341
SfaNI GCATC 1 cut(s) 442
SphI GCATGC 1 cut(s) 252
Sse9I AATT 3 cut(s) 325, 411, 539
SspI AATATT 1 cut(s) 60
SspMI CTAG 2 cut(s) 284, 459
SstI GAGCTC 1 cut(s) 204
StyI CCWWGG 1 cut(s) 283
TaqI TCGA 2 cut(s) 192, 269
TasI AATT 3 cut(s) 325, 411, 539
TatI WGTACW 2 cut(s) 348, 473
Tru1I TTAA 2 cut(s) 87, 410
Tru9I TTAA 2 cut(s) 87, 410
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 6 cut(s) 72, 252, 278, 314, 455, 499
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 3 cut(s) 151, 388, 528
XceI RCATGY 1 cut(s) 252
XmaJI CCTAGG 1 cut(s) 283
XspI CTAG 2 cut(s) 284, 459
ZrmI AGTACT 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.