pycom11g16520

Saccharopine dehydrogenase NADP binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011377
Physical Location & Seq
Forward (+)
18122432 .. 18128528
6097 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
ATGGCGGGAGCTTCAGTGCATATGCAGCATTTGAACAGCAGAGTCGGTGTTACTATGGCCACCGCGACTACTCCTACAGACACCCAAATCGGGACTCGGGTTCAACTCCCAGAGAAGACCCGCAATTCTCGGGTGTTGGTGCTGGGCGGAACGGGTCGGGTCGGAGGCTCCACTGCCATTGCCCTCTCCAAGCTATGCCCCGACCTCCAAATTGTCGTCGGCGGTCGAAACAGGGAAAAAGGTGTTAATATGGTAGCTACACTTGGGAGAAACTCAGAGTTCTCAGAAGTAGACATTAACAATGTAAAATCATTGGAAGCAGCTCTGAAAGATGTAGATCTCGTAGTTCATGCTGCAGGGCCATTTCAACAAGCAGAGAAGTGCACTGTATTGGAAGCTGCCTTAGAGACAAAGACAGCCTACGTTGATGTTTGTGATGACACAGCCTATTCACAACGTGCAAAATCCTTCAAGAACAGCGCTATAGCTGCAAATATTCCGGCTATAACAACTGGTGGAATTTATCCAGGAGTGAGCAATGTGATGGCTGCAGAACTAGTTCGTGCAGCTAGAAGTGAAAGCAAAGGCACACCCGAAAGGCTAAGATTCTATTACTACACAGCAGGAACTGGCGGCGCTGGTCCAACTATCTTAGCCACTAGCTTTTTGCTTCTGGGAGAGGAGGTTGTTGCATTTAATAAAGGAGAAAAGATCAAACTAAAGCCATATAGTGGAATGCTGGAGATTGACTTTGGAAAAGGCATTGGAAAGAAAGATGTTTATCTGTTGAATCTACCGGAGGTAAGAAGTACTCATGAGGTCCTAGGAGTACCTACTGTCAGTGCTCGATTTGGAACTTCACCCTTCTTTTGGAATTGGGGAATGGCAATCATGACTAGTCTTCTTCCACCGGAGCTATTGAGAGACAGAAGCAAAGTCCAACAGATGGTTGAACTGTTTGACCCCGTTGTCCGACAAGTTGATTCTTTTGCTGGGGAGCGTGTATCAATGAGGGTTGATTTGGAGTGCTCTGATGGGCGCAATACAGTTGGTATATTCAGTCACAAGAGACTCTCTGTATCTGTGGGATATGCAGCAGCTGCATTTGTTTTGGCAATTCTTGAGGGAAGCACAAAGCCTGGGGTTTGGTTTCCGGAGGAGAATGAAGGCATTGCAATTGAGGCAAGGGAACTCCTCCTCGAACGTGCTGCGCAAGGAACATTCAATTTCGTAATGAACAAGCCATCATGGATGGTGGAAACAAACCCAAAAGAGTTAGGGTTGGGAATATACGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sacchrp_dh_NADP

Protein Analysis

433

Amino Acids

46.61

Weight (kDa)

7.65

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 45 - 157 1e-17 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 968
Acc16I TGCGCA 1 cut(s) 1212
AccB7I CCANNNNNTGG 2 cut(s) 731, 946
AccI GTMKAC 1 cut(s) 291
AccII CGCG 1 cut(s) 65
AccIII TCCGGA 1 cut(s) 1153
AciI CCGC 6 cut(s) 5, 63, 121, 147, 222, 633
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 519
AdeI CACNNNGTG 1 cut(s) 458
AfaI GTAC 2 cut(s) 811, 831
AfeI AGCGCT 1 cut(s) 481
AfiI CCNNNNNNNGG 4 cut(s) 90, 731, 870, 946
AflIII ACRYGT 1 cut(s) 1293
AgsI TTSAA 7 cut(s) 34, 104, 368, 472, 790, 953, 1225
AhlI ACTAGT 2 cut(s) 556, 896
AjnI CCWGG 2 cut(s) 526, 1138
Alw21I GWGCWC 3 cut(s) 386, 847, 1031
Alw26I GTCTC 3 cut(s) 401, 918, 1063
Alw44I GTGCAC 1 cut(s) 382
AlwNI CAGNNNCTG 2 cut(s) 629, 1100
Ama87I CYCGRG 2 cut(s) 96, 129
Aor13HI TCCGGA 1 cut(s) 1153
Aor51HI AGCGCT 1 cut(s) 481
AoxI GGCC 2 cut(s) 57, 359
ApaLI GTGCAC 1 cut(s) 382
ApoI RAATTY 1 cut(s) 519
Asp700I GAANNNNTTC 1 cut(s) 558
AspA2I CCTAGG 1 cut(s) 823
AspLEI GCGC 4 cut(s) 482, 638, 1041, 1213
AspS9I GGNCC 3 cut(s) 359, 641, 820
AsuHPI GGTGA 1 cut(s) 852
AvaI CYCGRG 2 cut(s) 96, 129
AvaII GGWCC 2 cut(s) 641, 820
AvrII CCTAGG 1 cut(s) 823
BaeGI GKGCMC 1 cut(s) 386
BalI TGGCCA 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 122, 893
Bbv12I GWGCWC 3 cut(s) 386, 847, 1031
BccI CCATC 5 cut(s) 538, 940, 1028, 1246, 1252
BcgI CGANNNNNNTGC 2 cut(s) 1190, 1224
BciT130I CCWGG 2 cut(s) 528, 1140
BcoDI GTCTC 3 cut(s) 401, 918, 1063
BcuI ACTAGT 2 cut(s) 556, 896
BfaI CTAG 5 cut(s) 557, 570, 660, 824, 897
BfmI CTRYAG 4 cut(s) 75, 354, 483, 549
BfoI RGCGCY 2 cut(s) 483, 639
BglII AGATCT 1 cut(s) 337
BlnI CCTAGG 1 cut(s) 823
BmcAI AGTACT 1 cut(s) 811
Bme1390I CCNGG 2 cut(s) 528, 1140
Bme18I GGWCC 2 cut(s) 641, 820
BmeT110I CYCGRG 2 cut(s) 96, 129
BmgT120I GGNCC 3 cut(s) 359, 641, 820
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 2 cut(s) 528, 1140
BpiI GAAGAC 2 cut(s) 122, 893
BpmI CTGGAG 1 cut(s) 761
BpuEI CTTGAG 1 cut(s) 1142
BsaAI YACGTR 1 cut(s) 1294
BsaBI GATNNNNATC 2 cut(s) 336, 780
BsaJI CCNNGG 2 cut(s) 823, 1139
BsaWI WCCGGW 3 cut(s) 796, 910, 1153
BsaXI ACNNNNNCTCC 3 cut(s) 30, 669, 699
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 731, 870, 946
Bse1I ACTGG 2 cut(s) 517, 634
Bse3DI GCAATG 3 cut(s) 177, 544, 1170
Bse8I GATNNNNATC 2 cut(s) 336, 780
BseAI TCCGGA 1 cut(s) 1153
BseBI CCWGG 2 cut(s) 528, 1140
BseDI CCNNGG 2 cut(s) 823, 1139
BseGI GGATG 1 cut(s) 1257
BseJI GATNNNNATC 2 cut(s) 336, 780
BseLI CCNNNNNNNGG 4 cut(s) 90, 731, 870, 946
BseMI GCAATG 3 cut(s) 177, 544, 1170
BseMII CTCAG 2 cut(s) 288, 297
BseNI ACTGG 2 cut(s) 517, 634
BseRI GAGGAG 4 cut(s) 695, 1172, 1184, 1187
BseSI GKGCMC 1 cut(s) 386
BseYI CCCAGC 2 cut(s) 142, 992
BsgI GTGCAG 1 cut(s) 585
Bsh1236I CGCG 1 cut(s) 65
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 59, 361
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 3 cut(s) 386, 847, 1031
BsiHKCI CYCGRG 2 cut(s) 96, 129
BsiSI CCGG 4 cut(s) 500, 797, 911, 1154
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 4 cut(s) 90, 731, 870, 946
BsmAI GTCTC 3 cut(s) 401, 918, 1063
BsmFI GGGAC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 2 cut(s) 59, 361
BsoBI CYCGRG 2 cut(s) 96, 129
Bsp1286I GDGCHC 3 cut(s) 386, 847, 1031
Bsp13I TCCGGA 1 cut(s) 1153
Bsp143I GATC 2 cut(s) 337, 711
BspACI CCGC 6 cut(s) 5, 63, 121, 147, 222, 633
BspANI GGCC 2 cut(s) 59, 361
BspCNI CTCAG 2 cut(s) 287, 296
BspEI TCCGGA 1 cut(s) 1153
BspFNI CGCG 1 cut(s) 65
BspHI TCATGA 2 cut(s) 814, 891
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 2 cut(s) 358, 553
BsrDI GCAATG 3 cut(s) 177, 544, 1170
BsrI ACTGG 2 cut(s) 517, 634
BssECI CCNNGG 2 cut(s) 823, 1139
BssMI GATC 2 cut(s) 337, 711
BssT1I CCWWGG 1 cut(s) 823
Bst2UI CCWGG 2 cut(s) 528, 1140
Bst4CI ACNGT 4 cut(s) 388, 838, 957, 1048
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstBAI YACGTR 1 cut(s) 1294
BstDEI CTNAG 5 cut(s) 274, 283, 403, 602, 652
BstF5I GGATG 1 cut(s) 1257
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 2 cut(s) 483, 639
BstHHI GCGC 4 cut(s) 482, 638, 1041, 1213
BstKTI GATC 2 cut(s) 340, 714
BstMAI GTCTC 3 cut(s) 401, 918, 1063
BstMBI GATC 2 cut(s) 337, 711
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 25, 488, 1100, 1181
BstNI CCWGG 2 cut(s) 528, 1140
BstSCI CCNGG 2 cut(s) 526, 1138
BstSFI CTRYAG 4 cut(s) 75, 354, 483, 549
BstSLI GKGCMC 1 cut(s) 386
BstUI CGCG 1 cut(s) 65
BstV2I GAAGAC 2 cut(s) 122, 893
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 2 cut(s) 59, 361
BtsCI GGATG 1 cut(s) 1257
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 4 cut(s) 21, 171, 384, 847
CaiI CAGNNNCTG 2 cut(s) 629, 1100
CciI TCATGA 2 cut(s) 814, 891
CfoI GCGC 4 cut(s) 482, 638, 1041, 1213
Cfr13I GGNCC 3 cut(s) 359, 641, 820
Csp6I GTAC 2 cut(s) 810, 830
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 4 cut(s) 350, 815, 892, 1248
CviQI GTAC 2 cut(s) 810, 830
DdeI CTNAG 5 cut(s) 274, 283, 403, 602, 652
DpnI GATC 2 cut(s) 339, 713
DpnII GATC 2 cut(s) 337, 711
DraIII CACNNNGTG 1 cut(s) 458
DrdI GACNNNNNNGTC 1 cut(s) 968
DseDI GACNNNNNNGTC 1 cut(s) 968
EaeI YGGCCR 1 cut(s) 57
EciI GGCGGA 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 823
Eco47I GGWCC 2 cut(s) 641, 820
Eco47III AGCGCT 1 cut(s) 481
Eco88I CYCGRG 2 cut(s) 96, 129
EcoO109I RGGNCCY 1 cut(s) 820
EcoRII CCWGG 2 cut(s) 526, 1138
EcoT14I CCWWGG 1 cut(s) 823
ErhI CCWWGG 1 cut(s) 823
FaeI CATG 4 cut(s) 353, 818, 895, 1251
FaqI GGGAC 1 cut(s) 106
FatI CATG 4 cut(s) 349, 814, 891, 1247
FauI CCCGC 1 cut(s) 128
FauNDI CATATG 1 cut(s) 21
FblI GTMKAC 1 cut(s) 291
FokI GGATG 1 cut(s) 1264
FspBI CTAG 5 cut(s) 557, 570, 660, 824, 897
FspI TGCGCA 1 cut(s) 1212
GlaI GCGC 4 cut(s) 481, 637, 1040, 1212
GsaI CCCAGC 2 cut(s) 146, 996
GsuI CTGGAG 1 cut(s) 761
HaeII RGCGCY 2 cut(s) 483, 639
HaeIII GGCC 2 cut(s) 59, 361
HapII CCGG 4 cut(s) 500, 797, 911, 1154
HhaI GCGC 4 cut(s) 482, 638, 1041, 1213
Hin1II CATG 4 cut(s) 353, 818, 895, 1251
Hin6I GCGC 4 cut(s) 480, 636, 1039, 1211
HinP1I GCGC 4 cut(s) 480, 636, 1039, 1211
HinfI GANTC 6 cut(s) 42, 94, 606, 790, 983, 1071
HpaII CCGG 4 cut(s) 500, 797, 911, 1154
HphI GGTGA 1 cut(s) 852
Hpy166II GTNNAC 2 cut(s) 292, 384
Hpy188I TCNGA 6 cut(s) 164, 277, 286, 327, 974, 1033
Hpy188III TCNNGA 6 cut(s) 91, 472, 815, 892, 1121, 1154
Hpy8I GTNNAC 2 cut(s) 292, 384
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 478, 874, 1160
HpyCH4III ACNGT 4 cut(s) 388, 838, 957, 1048
HpyCH4IV ACGT 4 cut(s) 423, 457, 1204, 1293
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 488, 1100, 1181
HpyF3I CTNAG 5 cut(s) 274, 283, 403, 602, 652
HpySE526I ACGT 4 cut(s) 423, 457, 1204, 1293
Hsp92II CATG 4 cut(s) 353, 818, 895, 1251
HspAI GCGC 4 cut(s) 480, 636, 1039, 1211
Kpn2I TCCGGA 1 cut(s) 1153
Kzo9I GATC 2 cut(s) 337, 711
LmnI GCTCC 4 cut(s) 8, 173, 913, 997
MaeI CTAG 5 cut(s) 557, 570, 660, 824, 897
MaeII ACGT 4 cut(s) 423, 457, 1204, 1293
MaeIII GTNAC 2 cut(s) 49, 1061
MalI GATC 2 cut(s) 339, 713
MboI GATC 2 cut(s) 337, 711
MboII GAAGA 3 cut(s) 127, 893, 896
MfeI CAATTG 1 cut(s) 1176
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 3 cut(s) 386, 847, 1031
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 7 cut(s) 124, 210, 519, 874, 1116, 1176, 1225
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 3 cut(s) 51, 88, 1065
MmeI TCCRAC 4 cut(s) 142, 668, 964, 997
Mox20I TGGCCA 1 cut(s) 59
MroI TCCGGA 1 cut(s) 1153
MroXI GAANNNNTTC 1 cut(s) 558
MscI TGGCCA 1 cut(s) 59
MseI TTAA 3 cut(s) 246, 297, 696
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 1100
MspI CCGG 4 cut(s) 500, 797, 911, 1154
MspR9I CCNGG 2 cut(s) 528, 1140
MunI CAATTG 1 cut(s) 1176
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 2 cut(s) 528, 1140
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 4 cut(s) 25, 488, 1100, 1181
NdeI CATATG 1 cut(s) 21
NdeII GATC 2 cut(s) 337, 711
NlaIII CATG 4 cut(s) 353, 818, 895, 1251
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 1 cut(s) 1061
NsbI TGCGCA 1 cut(s) 1212
PagI TCATGA 2 cut(s) 814, 891
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 558
PfeI GAWTC 3 cut(s) 606, 790, 983
PflMI CCANNNNNTGG 2 cut(s) 731, 946
PfoI TCCNGGA 1 cut(s) 526
PleI GAGTC 3 cut(s) 50, 88, 1065
PpsI GAGTC 3 cut(s) 50, 88, 1065
Ppu21I YACGTR 1 cut(s) 1294
PpuMI RGGWCCY 1 cut(s) 820
Psp5II RGGWCCY 1 cut(s) 820
Psp6I CCWGG 2 cut(s) 526, 1138
PspFI CCCAGC 2 cut(s) 142, 992
PspGI CCWGG 2 cut(s) 526, 1138
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 3 cut(s) 359, 641, 820
PspPPI RGGWCCY 1 cut(s) 820
PstI CTGCAG 2 cut(s) 358, 553
PstNI CAGNNNCTG 2 cut(s) 629, 1100
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 1100
RsaI GTAC 2 cut(s) 811, 831
RsaNI GTAC 2 cut(s) 810, 830
SaqAI TTAA 3 cut(s) 246, 297, 696
Sau3AI GATC 2 cut(s) 337, 711
Sau96I GGNCC 3 cut(s) 359, 641, 820
ScaI AGTACT 1 cut(s) 811
SchI GAGTC 3 cut(s) 51, 88, 1065
ScrFI CCNGG 2 cut(s) 528, 1140
SduI GDGCHC 3 cut(s) 386, 847, 1031
SfcI CTRYAG 4 cut(s) 75, 354, 483, 549
SinI GGWCC 2 cut(s) 641, 820
SmlI CTYRAG 1 cut(s) 1121
SmoI CTYRAG 1 cut(s) 1121
SpeI ACTAGT 2 cut(s) 556, 896
Sse9I AATT 7 cut(s) 124, 210, 519, 874, 1116, 1176, 1225
SsiI CCGC 6 cut(s) 5, 63, 121, 147, 222, 633
SspI AATATT 1 cut(s) 496
SspMI CTAG 5 cut(s) 557, 570, 660, 824, 897
StyD4I CCNGG 2 cut(s) 526, 1138
StyI CCWWGG 1 cut(s) 823
TaaI ACNGT 4 cut(s) 388, 838, 957, 1048
TaiI ACGT 4 cut(s) 426, 460, 1207, 1296
TaqI TCGA 3 cut(s) 226, 847, 1200
TasI AATT 7 cut(s) 124, 210, 519, 874, 1116, 1176, 1225
TatI WGTACW 1 cut(s) 809
TauI GCSGC 1 cut(s) 636
TfiI GAWTC 3 cut(s) 606, 790, 983
Tru1I TTAA 3 cut(s) 246, 297, 696
Tru9I TTAA 3 cut(s) 246, 297, 696
TscAI CASTG 4 cut(s) 21, 178, 391, 847
TseFI GTSAC 1 cut(s) 1061
Tsp45I GTSAC 1 cut(s) 1061
TspDTI ATGAA 3 cut(s) 338, 1179, 1250
TspRI CASTG 4 cut(s) 21, 178, 391, 847
Van91I CCANNNNNTGG 2 cut(s) 731, 946
VneI GTGCAC 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 641, 820
XapI RAATTY 1 cut(s) 519
XmaJI CCTAGG 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 558
XspI CTAG 5 cut(s) 557, 570, 660, 824, 897
ZrmI AGTACT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.