pycom11g19800

disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009964
Physical Location & Seq
Forward (+)
22314170 .. 22322915
8746 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1443 bp
ATGATTTCCTCCGGCAAGATCAAGTCTGTCGATTTTTATAGGAAAATTCCTAGAGATTTGACAGAAGCATCATTATCGGGTGCAGGACTATCTATTGTAGCAGCTCTTGCCATGATGTTTTTATTTGGAATGGAACTGAATAATTATTTAACCGTCAGCACCTCTACATCTGTTATTGTCGACAAGAGTTCTGATGGGGATTTCTTACGCATTGAATTTAATATCAGTTTTCCTGCACTTTCATGTGAATTCGCATCTCTTGACGTGAGTGACGTGTTGGGAACAAACAGATTGAATATTACGAAAACAATTCGCAAGTTCTCCATAGGGACAGATTTAAGACCAACCGGCACTGAGTTCCACTCAGGACCCGTTTTACATAATATTAAGCATGGGGATGGAGATGAGTTTTCTGGTGATGGCTCTATTTCTCTAACTGCTCAGAATTTTGAAAAGTTTACTCACCAGCACCCAATTTTGGTGGTTAATTTTTATGCTCCGTGGTGCTACTGGAGCAATCGCCTGAAACCTTCATGGGAGAAGGCCGCCAAAATAATAAGAGAGAGGTATGATCGAGAAATAGATGGCCGTATACTTATGGCGAAGGTGGATTGTACTGAAGAAGGTGACTTGTGTAAGAGGAATCACATTCAAGGATATCCATCAATTCGTATTTTTCGTAAAGGAAGTGATGTGAGGGATGACCATGGACACCATGAGCATGAATCTTATTATGGTGATCGAGATACAGATAGCCTTGTTAAGACAATGGAAACTTTGGTTGCACCTATCCCTGTGGAGTCCGAAAAACTCGCTTTGGAGGGTAAATCTAACAACGGGGGTGAGAATGCAAAAAGACCAGCACCATTGACAGGAGGGTGTAGAATTGAAGGATATGTGCGTGTAAAGAAGGTTCCGGGAAACCTTGTCATTTCCGCTCATTCGGGAGCTCATTCCTTCGATGCTTCTCAAATGAACATGTCTCACGTCATTTCACATTTTTCATTCGGTAGGATGATTGCTCCTAAGGTGATGAGTGATGTCAAGCGATTGGTACCTTATCTTGGTGTGAGCCATGATAGGTTGAATGGTCGGTCTTTCATAAATCACCGTGACTTAGGTGCTAATGTTACAATAGAGCATTACCTTCAAATAGTTAAATCTGAAGTCATAACTGGGAGATCTCATAAATTAATTGAGGAATATGAGTACACGGCCCACAGCAGCTTGGCGCAGAGTCTCCAAATACCAGTTGCAAAGTTCCATTTCGAGCTCTCCCCCATGCAGGTTTTAATAACAGAAAACCCGAAATCCTTTTCACATTTTATCACGAATGTGTGCGCTATTATCGGAGGCGTTTTCACGGTAGCGGGAATAATGGACTCAATTCTGCACAATACTATTAGAATGATGAGGAAGGTTGAACTCGGCAAAAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003756 GO:0003824 GO:0016853 GO:0016860 GO:0016864 GO:0140096
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N Thioredoxin

Protein Analysis

481

Amino Acids

53.58

Weight (kDa)

7.74

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 97 3.1e-23 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
Thioredoxin PF00085 143 - 242 3.7e-18 Thioredoxin
COPIIcoated_ERV PF07970 289 - 461 1.2e-26 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50950 AT3G20560 AT4G27080
fragaria_vesca FvH4_3g21990 FvH4_3g21990 FvH4_3g21990
malus_domestica MD03G1209500.v1.1 MD11G1224300.v1.1
prunus_persica Prupe.4G205600_v2.0.a1
pyrus_communis pycom03g15830 pycom11g19790 pycom11g19800
rosa_chinensis RchiOBHm_Chr5g0037841
rosa_laevigata RLG00000033816
rosa_multiflora Rmu_sc0005416.1_g000015
rosa_roxburghii Rroxscaffold_1G00043080
rosa_rugosa Rorug05G0168000 Rorug05G0168100
rosa_samantha Rh5AG256900 Rh5DG269200
rosa_wichuraiana Rw5G023820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1276
Acc65I GGTACC 1 cut(s) 1054
AccB1I GGYRCC 1 cut(s) 1054
AccBSI CCGCTC 1 cut(s) 938
AccI GTMKAC 2 cut(s) 180, 592
AciI CCGC 3 cut(s) 546, 936, 1370
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 4 cut(s) 45, 215, 248, 445
AcuI CTGAAG 2 cut(s) 639, 1185
AfaI GTAC 3 cut(s) 616, 1056, 1211
AfiI CCNNNNNNNGG 4 cut(s) 478, 872, 1064, 1285
AflIII ACRYGT 2 cut(s) 273, 978
AgsI TTSAA 8 cut(s) 215, 295, 452, 653, 890, 1087, 1151, 1424
AjiI CACGTC 3 cut(s) 265, 274, 988
AleI CACNNNNGTG 1 cut(s) 1334
AluBI AGCT 4 cut(s) 104, 950, 1227, 1273
AluI AGCT 4 cut(s) 104, 950, 1227, 1273
Alw21I GWGCWC 2 cut(s) 952, 1275
Alw26I GTCTC 2 cut(s) 987, 1244
AoxI GGCC 3 cut(s) 543, 586, 1215
ApeKI GCWGC 2 cut(s) 101, 1224
ApoI RAATTY 4 cut(s) 45, 215, 248, 445
AseI ATTAAT 1 cut(s) 1193
Asp718I GGTACC 1 cut(s) 1054
AspLEI GCGC 2 cut(s) 1234, 1343
AspS9I GGNCC 2 cut(s) 368, 1216
AsuC2I CCSGG 1 cut(s) 918
AsuHPI GGTGA 7 cut(s) 428, 455, 638, 749, 854, 1042, 1100
AvaII GGWCC 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 1026
BanI GGYRCC 1 cut(s) 1054
BanII GRGCYC 2 cut(s) 952, 1275
Bbv12I GWGCWC 2 cut(s) 952, 1275
BbvI GCAGC 2 cut(s) 113, 1236
BccI CCATC 5 cut(s) 188, 392, 413, 578, 670
BceAI ACGGC 2 cut(s) 573, 1230
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BcnI CCSGG 1 cut(s) 918
BcoDI GTCTC 2 cut(s) 987, 1244
BfaI CTAG 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 1276
BglII AGATCT 1 cut(s) 1181
BisI GCNGC 3 cut(s) 102, 546, 1225
BlsI GCNGC 3 cut(s) 103, 547, 1226
Bme1390I CCNGG 1 cut(s) 918
Bme18I GGWCC 1 cut(s) 368
BmgBI CACGTC 3 cut(s) 265, 274, 988
BmgT120I GGNCC 2 cut(s) 368, 1216
BmiI GGNNCC 3 cut(s) 370, 915, 1056
BmrFI CCNGG 1 cut(s) 918
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 3 cut(s) 77, 263, 952
BmuI ACTGGG 1 cut(s) 1185
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 532
BpuMI CCSGG 1 cut(s) 918
BsaBI GATNNNNATC 1 cut(s) 661
BsaJI CCNNGG 2 cut(s) 500, 706
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 872, 1064, 1285
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 3 cut(s) 515, 1180, 1250
Bse21I CCTNAGG 1 cut(s) 1026
Bse8I GATNNNNATC 1 cut(s) 661
BseDI CCNNGG 2 cut(s) 500, 706
BseGI GGATG 3 cut(s) 403, 706, 1020
BseJI GATNNNNATC 1 cut(s) 661
BseLI CCNNNNNNNGG 4 cut(s) 478, 872, 1064, 1285
BseMII CTCAG 3 cut(s) 345, 378, 455
BseNI ACTGG 3 cut(s) 515, 1180, 1250
BseXI GCAGC 2 cut(s) 113, 1236
BsgI GTGCAG 3 cut(s) 102, 219, 1376
BshFI GGCC 3 cut(s) 545, 588, 1217
BshNI GGYRCC 1 cut(s) 1054
BsiHKAI GWGCWC 2 cut(s) 952, 1275
BsiSI CCGG 3 cut(s) 12, 348, 917
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 4 cut(s) 478, 872, 1064, 1285
BsmAI GTCTC 2 cut(s) 987, 1244
BsmFI GGGAC 1 cut(s) 343
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 3 cut(s) 545, 588, 1217
Bsp1286I GDGCHC 2 cut(s) 952, 1275
Bsp143I GATC 4 cut(s) 18, 571, 739, 1181
Bsp19I CCATGG 1 cut(s) 706
BspACI CCGC 3 cut(s) 546, 936, 1370
BspANI GGCC 3 cut(s) 545, 588, 1217
BspCNI CTCAG 3 cut(s) 346, 377, 454
BspLI GGNNCC 3 cut(s) 370, 915, 1056
BspMI ACCTGC 1 cut(s) 1276
BspT107I GGYRCC 1 cut(s) 1054
BsrBI CCGCTC 1 cut(s) 938
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 3 cut(s) 515, 1180, 1250
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 2 cut(s) 500, 706
BssMI GATC 4 cut(s) 18, 571, 739, 1181
BssNAI GTATAC 1 cut(s) 593
BssT1I CCWWGG 1 cut(s) 706
Bst1107I GTATAC 1 cut(s) 593
Bst4CI ACNGT 3 cut(s) 154, 1112, 1366
BstAPI GCANNNNNTGC 1 cut(s) 107
BstDEI CTNAG 5 cut(s) 354, 364, 441, 1026, 1117
BstDSI CCRYGG 2 cut(s) 500, 706
BstF5I GGATG 3 cut(s) 403, 706, 1020
BstHHI GCGC 2 cut(s) 1234, 1343
BstKTI GATC 4 cut(s) 21, 574, 742, 1184
BstMAI GTCTC 2 cut(s) 987, 1244
BstMBI GATC 4 cut(s) 18, 571, 739, 1181
BstMWI GCNNNNNNNGC 2 cut(s) 107, 513
BstNSI RCATGY 1 cut(s) 982
BstSCI CCNGG 1 cut(s) 916
BstV1I GCAGC 2 cut(s) 113, 1236
BstX2I RGATCY 1 cut(s) 1181
BstYI RGATCY 1 cut(s) 1181
BstZ17I GTATAC 1 cut(s) 593
Bsu36I CCTNAGG 1 cut(s) 1026
BsuRI GGCC 3 cut(s) 545, 588, 1217
BtgI CCRYGG 2 cut(s) 500, 706
BtrI CACGTC 3 cut(s) 265, 274, 988
BtsCI GGATG 3 cut(s) 403, 706, 1020
BtsIMutI CAGTG 1 cut(s) 351
BveI ACCTGC 1 cut(s) 1276
CfoI GCGC 2 cut(s) 1234, 1343
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 368, 1216
Csp6I GTAC 3 cut(s) 615, 1055, 1210
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviQI GTAC 3 cut(s) 615, 1055, 1210
DdeI CTNAG 5 cut(s) 354, 364, 441, 1026, 1117
DpnI GATC 4 cut(s) 20, 573, 741, 1183
DpnII GATC 4 cut(s) 18, 571, 739, 1181
EaeI YGGCCR 1 cut(s) 586
Ecl136II GAGCTC 2 cut(s) 950, 1273
Eco130I CCWWGG 1 cut(s) 706
Eco24I GRGCYC 2 cut(s) 952, 1275
Eco32I GATATC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 368
Eco53kI GAGCTC 2 cut(s) 950, 1273
Eco57I CTGAAG 2 cut(s) 639, 1185
Eco81I CCTNAGG 1 cut(s) 1026
EcoICRI GAGCTC 2 cut(s) 950, 1273
EcoO109I RGGNCCY 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 248
EcoRV GATATC 1 cut(s) 659
EcoT14I CCWWGG 1 cut(s) 706
EcoT38I GRGCYC 2 cut(s) 952, 1275
ErhI CCWWGG 1 cut(s) 706
FaqI GGGAC 1 cut(s) 343
FauI CCCGC 1 cut(s) 1363
FblI GTMKAC 2 cut(s) 180, 592
Fnu4HI GCNGC 3 cut(s) 102, 546, 1225
FokI GGATG 3 cut(s) 410, 713, 1027
FriOI GRGCYC 2 cut(s) 952, 1275
Fsp4HI GCNGC 3 cut(s) 102, 546, 1225
FspBI CTAG 1 cut(s) 51
GlaI GCGC 2 cut(s) 1233, 1342
GluI GCNGC 3 cut(s) 102, 546, 1225
GsuI CTGGAG 1 cut(s) 532
HaeIII GGCC 3 cut(s) 545, 588, 1217
HapII CCGG 3 cut(s) 12, 348, 917
HhaI GCGC 2 cut(s) 1234, 1343
Hin6I GCGC 2 cut(s) 1232, 1341
HinP1I GCGC 2 cut(s) 1232, 1341
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 5 cut(s) 643, 725, 800, 1237, 1382
HpaII CCGG 3 cut(s) 12, 348, 917
HphI GGTGA 7 cut(s) 428, 455, 638, 749, 854, 1042, 1100
Hpy166II GTNNAC 4 cut(s) 181, 459, 593, 1212
Hpy188I TCNGA 6 cut(s) 193, 444, 805, 1165, 1352, 1442
Hpy188III TCNNGA 6 cut(s) 260, 366, 575, 743, 945, 1330
Hpy8I GTNNAC 4 cut(s) 181, 459, 593, 1212
HpyAV CCTTC 9 cut(s) 535, 540, 598, 617, 884, 904, 967, 1157, 1411
HpyCH4III ACNGT 3 cut(s) 154, 1112, 1366
HpyCH4IV ACGT 3 cut(s) 264, 273, 987
HpyCH4V TGCA 7 cut(s) 83, 236, 785, 851, 1256, 1285, 1393
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 513
HpyF3I CTNAG 5 cut(s) 354, 364, 441, 1026, 1117
HpySE526I ACGT 3 cut(s) 264, 273, 987
HspAI GCGC 2 cut(s) 1232, 1341
KpnI GGTACC 1 cut(s) 1058
Kzo9I GATC 4 cut(s) 18, 571, 739, 1181
LmnI GCTCC 4 cut(s) 502, 513, 947, 1027
Lsp1109I GCAGC 2 cut(s) 113, 1236
LweI GCATC 3 cut(s) 77, 263, 952
MaeI CTAG 1 cut(s) 51
MaeII ACGT 3 cut(s) 264, 273, 987
MaeIII GTNAC 4 cut(s) 269, 626, 1112, 1129
MalI GATC 4 cut(s) 20, 573, 741, 1183
MbiI CCGCTC 1 cut(s) 938
MboI GATC 4 cut(s) 18, 571, 739, 1181
MboII GAAGA 1 cut(s) 632
MflI RGATCY 1 cut(s) 1181
MhlI GDGCHC 2 cut(s) 952, 1275
MlyI GAGTC 3 cut(s) 809, 1246, 1376
MseI TTAA 9 cut(s) 149, 219, 338, 387, 486, 762, 1158, 1193, 1292
MslI CAYNNNNRTG 4 cut(s) 241, 396, 720, 1334
MspI CCGG 3 cut(s) 12, 348, 917
MspR9I CCNGG 1 cut(s) 918
Mva1269I GAATGC 1 cut(s) 853
MwoI GCNNNNNNNGC 2 cut(s) 107, 513
NciI CCSGG 1 cut(s) 918
NcoI CCATGG 1 cut(s) 706
NdeII GATC 4 cut(s) 18, 571, 739, 1181
NlaIV GGNNCC 3 cut(s) 370, 915, 1056
NmeAIII GCCGAG 1 cut(s) 1407
NmuCI GTSAC 3 cut(s) 269, 626, 1112
NspI RCATGY 1 cut(s) 982
OliI CACNNNNGTG 1 cut(s) 1334
PciI ACATGT 1 cut(s) 978
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 676
PctI GAATGC 1 cut(s) 853
PfeI GAWTC 2 cut(s) 643, 725
PfoI TCCNGGA 1 cut(s) 916
PkrI GCNGC 3 cut(s) 103, 547, 1226
PleI GAGTC 3 cut(s) 808, 1245, 1376
PpsI GAGTC 3 cut(s) 808, 1245, 1376
PpuMI RGGWCCY 1 cut(s) 368
PscI ACATGT 1 cut(s) 978
PshBI ATTAAT 1 cut(s) 1193
Psp124BI GAGCTC 2 cut(s) 952, 1275
Psp5II RGGWCCY 1 cut(s) 368
PspN4I GGNNCC 3 cut(s) 370, 915, 1056
PspPI GGNCC 2 cut(s) 368, 1216
PspPPI RGGWCCY 1 cut(s) 368
PsrI GAACNNNNNNTAC 2 cut(s) 897, 929
PsuI RGATCY 1 cut(s) 1181
RsaI GTAC 3 cut(s) 616, 1056, 1211
RsaNI GTAC 3 cut(s) 615, 1055, 1210
RseI CAYNNNNRTG 4 cut(s) 241, 396, 720, 1334
SacI GAGCTC 2 cut(s) 952, 1275
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 9 cut(s) 149, 219, 338, 387, 486, 762, 1158, 1193, 1292
SatI GCNGC 3 cut(s) 102, 546, 1225
Sau3AI GATC 4 cut(s) 18, 571, 739, 1181
Sau96I GGNCC 2 cut(s) 368, 1216
SchI GAGTC 3 cut(s) 809, 1246, 1376
ScrFI CCNGG 1 cut(s) 918
SduI GDGCHC 2 cut(s) 952, 1275
SfaNI GCATC 3 cut(s) 77, 263, 952
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 4 cut(s) 241, 396, 720, 1334
SsiI CCGC 3 cut(s) 546, 936, 1370
SspI AATATT 2 cut(s) 298, 385
SspMI CTAG 1 cut(s) 51
SstI GAGCTC 2 cut(s) 952, 1275
StyD4I CCNGG 1 cut(s) 916
StyI CCWWGG 1 cut(s) 706
TaaI ACNGT 3 cut(s) 154, 1112, 1366
TaiI ACGT 3 cut(s) 267, 276, 990
TaqI TCGA 6 cut(s) 30, 180, 574, 742, 960, 1269
TaqII GACCGA 1 cut(s) 1083
TatI WGTACW 2 cut(s) 614, 1209
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 2 cut(s) 643, 725
Tru1I TTAA 9 cut(s) 149, 219, 338, 387, 486, 762, 1158, 1193, 1292
Tru9I TTAA 9 cut(s) 149, 219, 338, 387, 486, 762, 1158, 1193, 1292
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 3 cut(s) 269, 626, 1112
TseI GCWGC 2 cut(s) 101, 1224
Tsp45I GTSAC 3 cut(s) 269, 626, 1112
TspDTI ATGAA 6 cut(s) 231, 522, 738, 989, 993, 1090
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 368
VspI ATTAAT 1 cut(s) 1193
XapI RAATTY 4 cut(s) 45, 215, 248, 445
XceI RCATGY 1 cut(s) 982
XmiI GTMKAC 2 cut(s) 180, 592
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.