Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0014306

Physical Location & Seq

Genomic Coordinates Forward (+)

23771388 .. 23779099

Gene Structure 7712 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2706 bp

ATGTTATTTGTTTACTCTCAATCCTGGACTACCAAATTGTTTAAACCTCCCAAAAAGCCCAGTCCTCCAACCATTGTCCTTCTCGAGAGTCGAGACGAGACGAGGAAGACCAGAGAAATTTCAAAACGTATTCCCTGTTGCTTTTGTATTCAAAAAGCAAAAGCATACAGAAAACTTTGCACTGGTTTGGGGCAAGGAATCATATTCTCTCTCTCCCTCTTTCTCTCTCGTCTTGCCTGTCCAATTTCAAAACCCTTTCTTTCTGCACAGAGAGCGATGCTCGACCCCATGGACCTGCTCCGATCCAATCTATCCCGGGTTCGGATCCCGGAACCCACCAACCGCATCTACAAGCAAGAATGCTGCATCTCTTTCGACACTCCGAGATCAGAAGGTGGTTTGTTTGTGGATATGAACACATTTCTTGCTTTTGGAAAGGATTGTGTGGGCTGGAACTACGAAAAAACTGGAAATCCAGTTTATTTGCATATAAAGCAAACAAAAGTGTTGGTTCCTGAAGATAGGCCTTTGAAGAAACCGACACTCATGGCTATAGGTGTCGATGGGGGATTTGATAACAATGAACCTGAATATGAAGAAACGCACAGTATAGTCCTATTGCCTGATTATGTAACTCTTCCATTCCCATCTGTCGAATTACCAGAGAAGGTAAGATTGGCAGTTGATGCTATTTTATTGGCTGAGGGTGCTGAGAGGAAAGAACAACTTGCAGCATGGACAGCTGAGAAGAAGCAAGTTAGTGCTTATGCAACAAATTTGCAACAGATTGGAAATGGTATTATTATTCCTTCATCTGGTTGGAAATGTTGCAAGTGTGATAAAAGGGAAAATCTTTGGTTAAATCTAACTGATGGAATGATCCTTTGTGGGAGAAAAAATTGGGATGGAACTGGTGGCAACAACCATGCCATTGAACACTATAAGGAGACGGGCTATCCTCTTGCTGTAAAGCTGGGGACAATTACAGCTGATCTGGAAGGAGCAGATGTTTTTTCATACCCAGAGGATGATAGTGTATTAGACCCACTTTTAGCGCAACATCTGGCATTTTTTGGCATTGATTTCTCATCGTTACAGAAGACAGAAATGACAACTGCCGAAAGAGAGCTTGACCAGAATACCAATTTTGACTGGAACCGGATTCAAGAAAGTGGACAGGAAGTTGAACCGATATTTGGACCCGGTTACACTGGACTTGTGAATCTTGGAAACAGCTGCTATATGGCAGCGACTATGCAAGTCGTGTTTTCAACGCACTCTTTCTATTCCGGATACTTCAAGAATCAAAGTTTGAAAAAGGCTTTTGAGAAAGCACCAGCTGACCCCACTGTAGACCTAAACATGCAGTTAACAAAGCTGGCACATGGTTTGCTGTCTGGTAAATACTCTATTCCTGTAACCGAGGAAAAAGATCATACAAATGCTGCTGCCCCTACAACAAAATCAAAACAGGAAGGCATACCCCCTCGTATGTTTAAAGCGGTTATAGCTGCCAGCCATCCGGAATTTTCCTCCATGAGACAACAGCATATGATGCCTTGGAGTTCTTCCTTCATTTTCTTGACCAAGTTGAACGAGTTCATTCTGGAAGACCTGAGGTGGATCCTGCTAGGAGTTTTAAGTTTGGCATTGAACGATCGTATTATGTGTTCATCTGGGAAGGTTGCTTACCATAGAAGGCTTGACTACATTCTCTCATTAAACATTCCACTTCATGCAGCCACTAATAAAGAAGAACTTGAAGCATTTCAGAAATTGAAAGCTGAAAAGATTGCGGAAGGCAAGGAAGTATCCAGTGATGAGATTGTACGTCCAAGGGTTCCTCTAGAAGCATGCCTTGCAAGCTTTTCATCTCCAGAAGAGATACAGGATTTTTACAGCACTGCCTTGAAGGCCAAGACAACAGCTGTGAAGAGTACAGGTCTAACTTCATTCCCTGATTATCTGGTGTTGCACATGCGAAAGTTTGTAATGGAAGTGGGCTGGGTGCCAAAAAAACTTGATGTATATATAGATGTTCCTGATATCATTGATATCAGTGACATGCGGAGCAAGGGACTCCAACCTGGTGAAGAGTTGTTGCCGGAGGGTGGAGAAGAGGAGGAACCGAATAAGCCTGTTGCCGACGAGAGTATTGTTTCCCAGCTTGTTTCAATGGGTTTCAACCATTTACACTGTCAGAAAGCGGCCATTAATACGTCGAATGCCGGAGTGGAAGAGGCAATGAATTGGCTACTTTCTCACATGGATGATGCAGATATAGATACTCCTATTTCTCAAGGAGGACAGTGTGTGGTTGACCAATCAAAAGTGGATATGTTGATTTCATTTGGTTTTCAAGAAGACATTGCTCGGAAGGCACTGAAAGCATCGGGTGGTGACATCGAGAAAGCCACAGATTGGATATTCAACAATCCTGATGCTTCTGTTTCATCGGACATGGATGCGACAACATCTGATGCTGCATCTACTGCGATTGATGCTGGACTGCCTGATGGAGCAGGAAGATATAGACTCATGGGAATTGTAAGCCACATCGGAACATCTACTCAATGTGGTCATTATGTTGCGCACATCTTAAAAGATGGCAGATGGGTTATTTTCAATGACAGCAAAGTTGGAGCTTCCGTTAATCCTCCCAAGGACATGGGGTACCTTTACTTCTTTGAGAGGCTTAATAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0016049 GO:0016579 GO:0016787 GO:0019538 GO:0019783 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0036211 GO:0036459 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0070011 GO:0070646 GO:0070647 GO:0071695 GO:0071704 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0101005 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UBA UCH zf-UBP

Protein Analysis

902

Amino Acids

100.17

Weight (kDa)

5.55

Isoelectric Point (pI)

38.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP_var	PF17807	107 - 168	6.2e-22	Variant UBP zinc finger
zf-UBP	PF02148	276 - 349	1.2e-17	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	404 - 896	2.6e-35	Ubiquitin carboxyl-terminal hydrolase
UBA_7	PF22562	716 - 762	1.5e-20	UBA-like domain
UBA	PF00627	716 - 752	1.9e-07	UBA/TS-N domain
UBA	PF00627	773 - 809	5.4e-09	UBA/TS-N domain
UBA_7	PF22562	774 - 816	1e-10	UBA-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20630
fragaria_vesca	FvH4_3g21160 FvH4_3g21160
malus_domestica	MD03G1217200.v1.1 MD11G1234900.v1.1
prunus_persica	Prupe.4G192200_v2.0.a1
pyrus_communis	pycom11g20650
rosa_chinensis	RchiOBHm_Chr5g0036241
rosa_laevigata	RLG00000033681
rosa_multiflora	Rmu_sc0000247.1_g000007
rosa_roxburghii	Rroxscaffold_1G00044540
rosa_rugosa	Rorug05G0154300
rosa_samantha	Rh5AG248000 Rh5BG248600 Rh5CG279900 Rh5DG256900
rosa_wichuraiana	Rw5G022740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2592
Acc36I	ACCTGC	1 cut(s)	303
Acc65I	GGTACC	1 cut(s)	2673
AccB1I	GGYRCC	2 cut(s)	2008, 2673
AccB7I	CCANNNNNTGG	1 cut(s)	2421
AccI	GTMKAC	1 cut(s)	1353
AccIII	TCCGGA	2 cut(s)	1289, 1522
AciI	CCGC	5 cut(s)	343, 1502, 1796, 2068, 2207
AclWI	GGATC	6 cut(s)	297, 319, 332, 874, 1618, 1631
AcoI	YGGCCR	1 cut(s)	2208
AcsI	RAATTY	3 cut(s)	117, 775, 1526
AcuI	CTGAAG	1 cut(s)	537
AfaI	GTAC	3 cut(s)	1830, 1939, 2675
AfiI	CCNNNNNNNGG	5 cut(s)	321, 815, 1196, 2111, 2421
AjnI	CCWGG	2 cut(s)	23, 2086
AjuI	GAANNNNNNNTTGG	4 cut(s)	659, 691, 1179, 1211
Alw26I	GTCTC	4 cut(s)	87, 92, 941, 1534
AlwI	GGATC	6 cut(s)	297, 319, 332, 874, 1618, 1631
Ama87I	CYCGRG	2 cut(s)	83, 315
Aor13HI	TCCGGA	2 cut(s)	1289, 1522
AoxI	GGCC	3 cut(s)	524, 1914, 2208
ApeKI	GCWGC	9 cut(s)	363, 731, 1236, 1247, 1445, 1448, 1511, 1739, 2483
ApoI	RAATTY	3 cut(s)	117, 775, 1526
AseI	ATTAAT	1 cut(s)	2214
Asp700I	GAANNNNTTC	2 cut(s)	1598, 1767
Asp718I	GGTACC	1 cut(s)	2673
AspLEI	GCGC	2 cut(s)	1057, 2593
AspS9I	GGNCC	2 cut(s)	292, 1199
AsuC2I	CCSGG	4 cut(s)	316, 317, 329, 1203
AsuHPI	GGTGA	2 cut(s)	2102, 2411
AvaI	CYCGRG	2 cut(s)	83, 315
AvaII	GGWCC	2 cut(s)	292, 1199
AxyI	CCTNAGG	1 cut(s)	1616
BamHI	GGATCC	2 cut(s)	324, 1623
BanI	GGYRCC	2 cut(s)	2008, 2673
BarI	GAAGNNNNNNTAC	4 cut(s)	1673, 1705, 2666, 2698
BbsI	GAAGAC	4 cut(s)	113, 1106, 1617, 2370
BbvCI	CCTCAGC	1 cut(s)	702
BbvI	GCAGC	9 cut(s)	350, 743, 1223, 1259, 1432, 1435, 1498, 1751, 2470
BccI	CCATC	8 cut(s)	557, 655, 866, 899, 1527, 2510, 2600, 2607
BcgI	CGANNNNNNTGC	2 cut(s)	355, 389
BciT130I	CCWGG	2 cut(s)	25, 2088
BciVI	GTATCC	2 cut(s)	1286, 1822
BcnI	CCSGG	4 cut(s)	316, 317, 329, 1203
BcoDI	GTCTC	4 cut(s)	87, 92, 941, 1534
BfaI	CTAG	2 cut(s)	1631, 1847
BfmI	CTRYAG	2 cut(s)	552, 1350
BfuAI	ACCTGC	1 cut(s)	303
BfuI	GTATCC	2 cut(s)	1286, 1822
BglI	GCCNNNNNGGC	1 cut(s)	1913
Bme1390I	CCNGG	6 cut(s)	25, 316, 317, 329, 1203, 2088
Bme18I	GGWCC	2 cut(s)	292, 1199
BmeT110I	CYCGRG	2 cut(s)	83, 315
BmgT120I	GGNCC	2 cut(s)	292, 1199
BmrFI	CCNGG	6 cut(s)	25, 316, 317, 329, 1203, 2088
BmrI	ACTGGG	1 cut(s)	54
BmuI	ACTGGG	1 cut(s)	54
BpiI	GAAGAC	4 cut(s)	113, 1106, 1617, 2370
BplI	GAGNNNNNCTC	2 cut(s)	264, 296
BpmI	CTGGAG	1 cut(s)	1860
Bpu10I	CCTNAGC	1 cut(s)	702
BpuEI	CTTGAG	1 cut(s)	2283
BpuMI	CCSGG	4 cut(s)	316, 317, 329, 1203
BsaJI	CCNNGG	6 cut(s)	288, 315, 1422, 1559, 1835, 2661
BsaWI	WCCGGW	3 cut(s)	1158, 1289, 1522
BsaXI	ACNNNNNCTCC	4 cut(s)	993, 1023, 2634, 2664
Bsc4I	CCNNNNNNNGG	5 cut(s)	321, 815, 1196, 2111, 2421
Bse1I	ACTGG	8 cut(s)	60, 187, 472, 476, 916, 1157, 1216, 1815
Bse21I	CCTNAGG	1 cut(s)	1616
Bse3DI	GCAATG	2 cut(s)	2250, 2367
BseAI	TCCGGA	2 cut(s)	1289, 1522
BseBI	CCWGG	2 cut(s)	25, 2088
BseDI	CCNNGG	6 cut(s)	288, 315, 1422, 1559, 1835, 2661
BseGI	GGATG	5 cut(s)	910, 1033, 1519, 2275, 2470
BseLI	CCNNNNNNNGG	5 cut(s)	321, 815, 1196, 2111, 2421
BseMI	GCAATG	2 cut(s)	2250, 2367
BseMII	CTCAG	4 cut(s)	693, 702, 735, 1607
BseNI	ACTGG	8 cut(s)	60, 187, 472, 476, 916, 1157, 1216, 1815
BseRI	GAGGAG	1 cut(s)	2135
BseXI	GCAGC	9 cut(s)	350, 743, 1223, 1259, 1432, 1435, 1498, 1751, 2470
BseYI	CCCAGC	3 cut(s)	973, 2004, 2163
BsgI	GTGCAG	1 cut(s)	249
Bsh1285I	CGRYCG	1 cut(s)	1660
BshFI	GGCC	3 cut(s)	526, 1916, 2210
BshNI	GGYRCC	2 cut(s)	2008, 2673
BsiEI	CGRYCG	1 cut(s)	1660
BsiHKCI	CYCGRG	2 cut(s)	83, 315
BsiSI	CCGG	8 cut(s)	316, 329, 1159, 1203, 1290, 1523, 2105, 2229
BslFI	GGGAC	2 cut(s)	991, 2091
BslI	CCNNNNNNNGG	5 cut(s)	321, 815, 1196, 2111, 2421
BsmAI	GTCTC	4 cut(s)	87, 92, 941, 1534
BsmBI	CGTCTC	3 cut(s)	87, 92, 941
BsmFI	GGGAC	2 cut(s)	991, 2091
BsmI	GAATGC	2 cut(s)	365, 2230
BsnI	GGCC	3 cut(s)	526, 1916, 2210
BsoBI	CYCGRG	2 cut(s)	83, 315
Bsp13I	TCCGGA	2 cut(s)	1289, 1522
Bsp143I	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
Bsp19I	CCATGG	1 cut(s)	288
BspACI	CCGC	5 cut(s)	343, 1502, 1796, 2068, 2207
BspANI	GGCC	3 cut(s)	526, 1916, 2210
BspCNI	CTCAG	4 cut(s)	694, 703, 736, 1608
BspEI	TCCGGA	2 cut(s)	1289, 1522
BspMI	ACCTGC	1 cut(s)	303
BspPI	GGATC	6 cut(s)	297, 319, 332, 874, 1618, 1631
BspT107I	GGYRCC	2 cut(s)	2008, 2673
BsrDI	GCAATG	2 cut(s)	2250, 2367
BsrI	ACTGG	8 cut(s)	60, 187, 472, 476, 916, 1157, 1216, 1815
BssECI	CCNNGG	6 cut(s)	288, 315, 1422, 1559, 1835, 2661
BssMI	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
BssT1I	CCWWGG	4 cut(s)	288, 1559, 1835, 2661
Bst2UI	CCWGG	2 cut(s)	25, 2088
Bst4CI	ACNGT	4 cut(s)	608, 1351, 2198, 2310
Bst6I	CTCTTC	6 cut(s)	642, 1875, 1928, 2088, 2112, 2232
BstAPI	GCANNNNNTGC	4 cut(s)	686, 1555, 1859, 2492
BstC8I	GCNNGC	4 cut(s)	1380, 1516, 1855, 1864
BstDEI	CTNAG	4 cut(s)	702, 711, 744, 1616
BstDSI	CCRYGG	1 cut(s)	288
BstF5I	GGATG	5 cut(s)	910, 1033, 1519, 2275, 2470
BstHHI	GCGC	2 cut(s)	1057, 2593
BstKTI	GATC	8 cut(s)	305, 327, 389, 882, 994, 1435, 1626, 1660
BstMAI	GTCTC	4 cut(s)	87, 92, 941, 1534
BstMBI	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
BstMCI	CGRYCG	1 cut(s)	1660
BstNI	CCWGG	2 cut(s)	25, 2088
BstNSI	RCATGY	4 cut(s)	1366, 1857, 1981, 2068
BstSCI	CCNGG	6 cut(s)	23, 314, 315, 327, 1201, 2086
BstSFI	CTRYAG	2 cut(s)	552, 1350
BstV1I	GCAGC	9 cut(s)	350, 743, 1223, 1259, 1432, 1435, 1498, 1751, 2470
BstV2I	GAAGAC	4 cut(s)	113, 1106, 1617, 2370
BstX2I	RGATCY	2 cut(s)	324, 1623
BstXI	CCANNNNNNTGG	1 cut(s)	2668
BstYI	RGATCY	2 cut(s)	324, 1623
Bsu36I	CCTNAGG	1 cut(s)	1616
BsuI	GTATCC	2 cut(s)	1286, 1822
BsuRI	GGCC	3 cut(s)	526, 1916, 2210
BtgI	CCRYGG	1 cut(s)	288
BtgZI	GCGATG	1 cut(s)	290
BtsCI	GGATG	5 cut(s)	910, 1033, 1519, 2275, 2470
BtsI	GCAGTG	1 cut(s)	1902
BtsIMutI	CAGTG	9 cut(s)	180, 1209, 1347, 1822, 1902, 2065, 2194, 2315, 2381
BveI	ACCTGC	1 cut(s)	303
Cac8I	GCNNGC	4 cut(s)	1380, 1516, 1855, 1864
CfoI	GCGC	2 cut(s)	1057, 2593
Cfr13I	GGNCC	2 cut(s)	292, 1199
Cfr9I	CCCGGG	1 cut(s)	315
CsiI	ACCWGGT	1 cut(s)	2086
Csp6I	GTAC	3 cut(s)	1829, 1938, 2674
CviQI	GTAC	3 cut(s)	1829, 1938, 2674
DdeI	CTNAG	4 cut(s)	702, 711, 744, 1616
DpnI	GATC	8 cut(s)	304, 326, 388, 881, 993, 1434, 1625, 1659
DpnII	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
DraI	TTTAAA	2 cut(s)	43, 1498
EaeI	YGGCCR	1 cut(s)	2208
Eam1104I	CTCTTC	6 cut(s)	642, 1875, 1928, 2088, 2112, 2232
EarI	CTCTTC	6 cut(s)	642, 1875, 1928, 2088, 2112, 2232
Eco130I	CCWWGG	4 cut(s)	288, 1559, 1835, 2661
Eco147I	AGGCCT	1 cut(s)	526
Eco32I	GATATC	2 cut(s)	2047, 2056
Eco47I	GGWCC	2 cut(s)	292, 1199
Eco57I	CTGAAG	1 cut(s)	537
Eco81I	CCTNAGG	1 cut(s)	1616
Eco88I	CYCGRG	2 cut(s)	83, 315
EcoRII	CCWGG	2 cut(s)	23, 2086
EcoRV	GATATC	2 cut(s)	2047, 2056
EcoT14I	CCWWGG	4 cut(s)	288, 1559, 1835, 2661
ErhI	CCWWGG	4 cut(s)	288, 1559, 1835, 2661
Esp3I	CGTCTC	3 cut(s)	87, 92, 941
FalI	AAGNNNNNCTT	6 cut(s)	711, 743, 1743, 1775, 1842, 1874
FaqI	GGGAC	2 cut(s)	991, 2091
FauNDI	CATATG	1 cut(s)	1551
FblI	GTMKAC	1 cut(s)	1353
FokI	GGATG	5 cut(s)	917, 1040, 1506, 2282, 2477
FspBI	CTAG	2 cut(s)	1631, 1847
FspI	TGCGCA	1 cut(s)	2592
GlaI	GCGC	2 cut(s)	1056, 2592
GsaI	CCCAGC	3 cut(s)	977, 2008, 2167
GsuI	CTGGAG	1 cut(s)	1860
HaeIII	GGCC	3 cut(s)	526, 1916, 2210
HapII	CCGG	8 cut(s)	316, 329, 1159, 1203, 1290, 1523, 2105, 2229
HhaI	GCGC	2 cut(s)	1057, 2593
Hin6I	GCGC	2 cut(s)	1055, 2591
HinP1I	GCGC	2 cut(s)	1055, 2591
HincII	GTYRAC	2 cut(s)	1371, 2320
HindII	GTYRAC	2 cut(s)	1371, 2320
HindIII	AAGCTT	1 cut(s)	1864
HinfI	GANTC	7 cut(s)	88, 198, 1162, 1222, 1303, 2079, 2535
HpaI	GTTAAC	1 cut(s)	1371
HpaII	CCGG	8 cut(s)	316, 329, 1159, 1203, 1290, 1523, 2105, 2229
HphI	GGTGA	2 cut(s)	2102, 2411
Hpy166II	GTNNAC	5 cut(s)	13, 1175, 1354, 1371, 2320
Hpy8I	GTNNAC	5 cut(s)	13, 1175, 1354, 1371, 2320
Hpy99I	CGWCG	2 cut(s)	2150, 2224
HpyCH4III	ACNGT	4 cut(s)	608, 1351, 2198, 2310
HpyCH4IV	ACGT	3 cut(s)	127, 1831, 2219
HpyF3I	CTNAG	4 cut(s)	702, 711, 744, 1616
HpySE526I	ACGT	3 cut(s)	127, 1831, 2219
HspAI	GCGC	2 cut(s)	1055, 2591
Kpn2I	TCCGGA	2 cut(s)	1289, 1522
KpnI	GGTACC	1 cut(s)	2677
KspAI	GTTAAC	1 cut(s)	1371
Kzo9I	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
LmnI	GCTCC	5 cut(s)	303, 1001, 2070, 2519, 2642
Lsp1109I	GCAGC	9 cut(s)	350, 743, 1223, 1259, 1432, 1435, 1498, 1751, 2470
MabI	ACCWGGT	1 cut(s)	2086
MaeI	CTAG	2 cut(s)	1631, 1847
MaeII	ACGT	3 cut(s)	127, 1831, 2219
MaeIII	GTNAC	6 cut(s)	631, 1092, 1205, 1417, 2060, 2399
MalI	GATC	8 cut(s)	304, 326, 388, 881, 993, 1434, 1625, 1659
MboI	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
MflI	RGATCY	2 cut(s)	324, 1623
MlyI	GAGTC	3 cut(s)	97, 2073, 2529
MmeI	TCCRAC	4 cut(s)	92, 800, 2107, 2620
MroI	TCCGGA	2 cut(s)	1289, 1522
MroXI	GAANNNNTTC	2 cut(s)	1598, 1767
MslI	CAYNNNNRTG	2 cut(s)	1440, 2268
MspA1I	CMGCKG	5 cut(s)	743, 989, 1236, 1340, 1928
MspI	CCGG	8 cut(s)	316, 329, 1159, 1203, 1290, 1523, 2105, 2229
MspR9I	CCNGG	6 cut(s)	25, 316, 317, 329, 1203, 2088
MssI	GTTTAAAC	1 cut(s)	43
Mva1269I	GAATGC	2 cut(s)	365, 2230
MvaI	CCWGG	2 cut(s)	25, 2088
NciI	CCSGG	4 cut(s)	316, 317, 329, 1203
NcoI	CCATGG	1 cut(s)	288
NdeI	CATATG	1 cut(s)	1551
NdeII	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
NmuCI	GTSAC	2 cut(s)	2060, 2399
NsbI	TGCGCA	1 cut(s)	2592
NspI	RCATGY	4 cut(s)	1366, 1857, 1981, 2068
PaeI	GCATGC	1 cut(s)	1857
PaeR7I	CTCGAG	1 cut(s)	83
PceI	AGGCCT	1 cut(s)	526
PctI	GAATGC	2 cut(s)	365, 2230
PdmI	GAANNNNTTC	2 cut(s)	1598, 1767
PfeI	GAWTC	4 cut(s)	198, 1162, 1222, 1303
PflMI	CCANNNNNTGG	1 cut(s)	2421
PfoI	TCCNGGA	2 cut(s)	23, 327
Ple19I	CGATCG	1 cut(s)	1660
PleI	GAGTC	3 cut(s)	96, 2073, 2529
PmeI	GTTTAAAC	1 cut(s)	43
PpsI	GAGTC	3 cut(s)	96, 2073, 2529
PshBI	ATTAAT	1 cut(s)	2214
Psp6I	CCWGG	2 cut(s)	23, 2086
PspFI	CCCAGC	3 cut(s)	973, 2004, 2163
PspGI	CCWGG	2 cut(s)	23, 2086
PspPI	GGNCC	2 cut(s)	292, 1199
PsrI	GAACNNNNNNTAC	2 cut(s)	1654, 1686
PsuI	RGATCY	2 cut(s)	324, 1623
PvuI	CGATCG	1 cut(s)	1660
PvuII	CAGCTG	5 cut(s)	743, 989, 1236, 1340, 1928
RsaI	GTAC	3 cut(s)	1830, 1939, 2675
RsaNI	GTAC	3 cut(s)	1829, 1938, 2674
RseI	CAYNNNNRTG	2 cut(s)	1440, 2268
Sau3AI	GATC	8 cut(s)	302, 324, 386, 879, 991, 1432, 1623, 1657
Sau96I	GGNCC	2 cut(s)	292, 1199
SchI	GAGTC	3 cut(s)	97, 2073, 2529
ScrFI	CCNGG	6 cut(s)	25, 316, 317, 329, 1203, 2088
SexAI	ACCWGGT	1 cut(s)	2086
SfcI	CTRYAG	2 cut(s)	552, 1350
Sfr274I	CTCGAG	1 cut(s)	83
SinI	GGWCC	2 cut(s)	292, 1199
SlaI	CTCGAG	1 cut(s)	83
SmaI	CCCGGG	1 cut(s)	317
SmiMI	CAYNNNNRTG	2 cut(s)	1440, 2268
SmlI	CTYRAG	2 cut(s)	83, 2298
SmoI	CTYRAG	2 cut(s)	83, 2298
SphI	GCATGC	1 cut(s)	1857
SseBI	AGGCCT	1 cut(s)	526
SsiI	CCGC	5 cut(s)	343, 1502, 1796, 2068, 2207
SspMI	CTAG	2 cut(s)	1631, 1847
StuI	AGGCCT	1 cut(s)	526
StyD4I	CCNGG	6 cut(s)	23, 314, 315, 327, 1201, 2086
StyI	CCWWGG	4 cut(s)	288, 1559, 1835, 2661
TaaI	ACNGT	4 cut(s)	608, 1351, 2198, 2310
TaiI	ACGT	3 cut(s)	130, 1834, 2222
TaqI	TCGA	8 cut(s)	84, 91, 282, 375, 561, 654, 2222, 2406
TatI	WGTACW	1 cut(s)	1937
TauI	GCSGC	1 cut(s)	2210
TfiI	GAWTC	4 cut(s)	198, 1162, 1222, 1303
TscAI	CASTG	9 cut(s)	187, 1216, 1354, 1822, 1909, 2065, 2201, 2315, 2388
TseFI	GTSAC	2 cut(s)	2060, 2399
TseI	GCWGC	9 cut(s)	363, 731, 1236, 1247, 1445, 1448, 1511, 1739, 2483
Tsp45I	GTSAC	2 cut(s)	2060, 2399
TspGWI	ACGGA	1 cut(s)	2638
TspMI	CCCGGG	1 cut(s)	315
TspRI	CASTG	9 cut(s)	187, 1216, 1354, 1822, 1909, 2065, 2201, 2315, 2388
Van91I	CCANNNNNTGG	1 cut(s)	2421
VpaK11BI	GGWCC	2 cut(s)	292, 1199
VspI	ATTAAT	1 cut(s)	2214
XapI	RAATTY	3 cut(s)	117, 775, 1526
XbaI	TCTAGA	1 cut(s)	1846
XceI	RCATGY	4 cut(s)	1366, 1857, 1981, 2068
XhoI	CTCGAG	1 cut(s)	83
XmaI	CCCGGG	1 cut(s)	315
XmiI	GTMKAC	1 cut(s)	1353
XmnI	GAANNNNTTC	2 cut(s)	1598, 1767
XspI	CTAG	2 cut(s)	1631, 1847

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.