pycom11g24510

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008556
Physical Location & Seq
Reverse (-)
27431957 .. 27435836
3880 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2292 bp
ATGAAGACTGGAAGAATGGTGCAAGAACAGAACCTGGAGAAGCAGATGGGGTGCATGGCTGGCTTCCTACAGATCTTCGATCGCCACCAGCTTCTCAACGGAAAACGCCTCAACTCCACCAAACGCCTCCCTCCCTCGGCGACGGTGGTATCGTCGCCAGAGTCGGAGTCGGCCATGTCGCGGGAGATATCCAAAGAATTGGAAAAACAACCGCAGAACAAGACGGCACCGTCACCGGACCGATCGAAGCAATCTCCATCTCCGACGAAAGATCTCCGATCTCCATCGCCAGAAACTCGCGCTCCTCCGTCACCGGAACCTCATCCGAAATCTCCTCTTCCGCTTCCAGTTTTCGAATTCAAAGAAGGCACGAGGTCCTCGTGGAAGTTCTCCAGAGAAGCACCGAGGCTCTCACTGGATAGCAGAGCCACCGTCGACGCCAAGGGAAGCCTATACCCGAGGGAGATCCGAACAAACGCCGCCATACTAACCGCCGGTCGGAGCTCCGCCGACGGAGGAAGCGATAGTGACAAGCAACACCGATCACCGAGCGTTATCGCTAGGCTAATGGGACTCGAACCGTTGCAGCAGTCGGATCCAGAACCGATTAAGCTCCGGAGATCTTCGTCCGAATCCAGAGTGAACCGAGACCTGAAACAGTACCGGTTCGTCGACGGCAACAATTACCAGCAGAAGCAACCGCCGCCACAGAATGTGCAGAGCCATAATTTCTCCGGCAATGTAGGCAAAGACGACCGGAGCTCCAATCGGGGCTCCTATCCGAGGCAGCCGGATCCGAAGAACAATGCGAAGGCTCAGACCAAAGGAATGGCGCAGAGAAAGTGCTTCTTCAACTCGGCGGACTTCTTCCCCGAGCCGAAGCAGAGAGCCTCCGTTTACAACGAGATTGAGAAGCGGTTGATGATGCGAGGAATCGACGAGCCGTCGAAAGATATGGAGACGTTGAAGGAAATCTTCGAAGCTCTGCAGCTCAAAGGCCTCCTGCGCTCCAGGAAAGCTGCCAATCAGATTAATTCCAGAAACTTCTTCTACGATCGGAGCTTCCAGTCGCCGATCGTAGTGATGAAGCCGTCCATATCGCCGAGCTCGGTTCACCGCGCAGCCAGATTCGGAAACGACTCGCCGCCGCCAAGTTCGGGATTTAGGTCGAGAGCCGGAGTTCGCCGAAACGGCGAGGTATCACCTGCGGTGAGTCCTAGGCGCGACCGGCCGGAGAATGCACGGAGCCAGACTAGAGGGAGGAGTCCTGCCGCGCGAGAGGGCGGCGTGAGGAGTCCGAGTCGGAGAGGAGGACCGTTATCGATCGAAACGCAGCGGAAGGGGAATGATTCGGTGGAGCAGAGGAGAGTATCTCCGGTGCATTCTCCTAAAGTCAGTTCGAGAAGACCAGATCAAACGCCCACGAATCGATCTCCCAGGTACATCAAGAAACCAACGGTTGAGATTGCTCCAAGTGAGGAAAAGGCCCTCCATCGGGCGGAGGACGAATCGTCCACCACCATGTCAGAGAGCAGCGTTAGCAACAGCACCTCGTCACAAGCCGAGACAGAGAGGTGGAAGGCGGAAGAGTACAAAGAAGGGAGGAGTTTGTTGGAGAGGTGTGATAAGCTGCTTCACAGCATAGCTGAGTTGCAACCGAGTCCTGTCTCGGTTCTCGACTCGTCGTTTTACAAAGAGGAATCTTCGCCTTCGCCAGTCATGAAACGCAGCATTGATTTCAAAGTTGAAATGGAAGACATTTGGGGCGAGGCAATGTGGGGCACTGAGTTCGAATCCGAAGACAGCGATTTCGTCTACGTATCGGAGATCCTGCGGGCTTCCACTTACTTACCGGAAGACTCCGACATTTTCCTATTACTAGAGAAGAAGCAACATTTCAATGGAAAAGACACCTCCAAAGAGTCAACACTCGAGAGAAGGATCATTTTCGACACCATCACCGAAATTCTAGACCGAAACCGCCAATTGCCGCCGTGGAAGGCGGTCTCGTGGCAAACTTCCTTGCCTGAAACTTGGTCCGAGTTTCAAAGAATGCGGGAGAGGGACGACTCCGAGGACTTGTTCAAGGTCATATGCGGGATGTTGAAAAAGGACCTTGCCGGCGACGCAATCAACGGATGGGGAGATTGCTCCATCGAAATGTCCGACGCCGTTTTGGACATCGAACGGCTCATTTTCAAGGACTTGATCGGGCAAACGATCCGAGATCTCGCCGCTTTTCCCACCAGCTACATCAAAGTTTCGGCGCCTTGCAGGAAGTTGGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

764

Amino Acids

86.16

Weight (kDa)

9.4

Isoelectric Point (pI)

79.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 603 - 737 2e-14 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1213
AasI GACNNNNNNGTC 1 cut(s) 229
Acc36I ACCTGC 1 cut(s) 1213
AccB1I GGYRCC 2 cut(s) 226, 2266
AccI GTMKAC 3 cut(s) 435, 672, 1815
AccII CGCG 6 cut(s) 181, 300, 1119, 1224, 1274, 1276
AccIII TCCGGA 1 cut(s) 615
AclWI GGATC 8 cut(s) 460, 590, 603, 788, 801, 1822, 1949, 2215
AcoI YGGCCR 2 cut(s) 171, 1229
AcsI RAATTY 2 cut(s) 356, 1965
AcyI GRCGYC 3 cut(s) 438, 2169, 2267
AfaI GTAC 3 cut(s) 662, 1442, 1592
AfiI CCNNNNNNNGG 8 cut(s) 136, 180, 498, 783, 1157, 1494, 1495, 1498
AgeI ACCGGT 1 cut(s) 663
AhdI GACNNNNNGTC 2 cut(s) 943, 1510
AjnI CCWGG 3 cut(s) 33, 1010, 1436
Alw21I GWGCWC 3 cut(s) 506, 764, 1109
Alw26I GTCTC 5 cut(s) 642, 953, 1559, 1672, 2011
AlwI GGATC 8 cut(s) 460, 590, 603, 788, 801, 1822, 1949, 2215
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 3 cut(s) 457, 872, 1931
Aor13HI TCCGGA 1 cut(s) 615
AoxI GGCC 4 cut(s) 171, 997, 1229, 1485
ApeKI GCWGC 9 cut(s) 586, 787, 988, 1019, 1121, 1333, 1533, 1630, 1728
ApoI RAATTY 2 cut(s) 356, 1965
ArsI GACNNNNNNTTYG 2 cut(s) 1793, 1825
AseI ATTAAT 1 cut(s) 1032
AsiGI ACCGGT 1 cut(s) 663
AspA2I CCTAGG 1 cut(s) 1217
AspLEI GCGC 7 cut(s) 302, 835, 1008, 1121, 1224, 1276, 2269
AspS9I GGNCC 6 cut(s) 238, 375, 1313, 1486, 2037, 2113
AsuHPI GGTGA 7 cut(s) 225, 303, 537, 1106, 1194, 1222, 1951
AsuII TTCGAA 3 cut(s) 354, 978, 1791
AvaI CYCGRG 3 cut(s) 457, 872, 1931
AvaII GGWCC 5 cut(s) 238, 375, 1313, 2037, 2113
AvrII CCTAGG 1 cut(s) 1217
BaeGI GKGCMC 1 cut(s) 1784
BaeI ACNNNNGTAYC 2 cut(s) 132, 165
BamHI GGATCC 2 cut(s) 595, 793
BanI GGYRCC 2 cut(s) 226, 2266
BanII GRGCYC 4 cut(s) 506, 764, 776, 1109
BauI CACGAG 3 cut(s) 370, 379, 2008
BbsI GAAGAC 6 cut(s) 11, 1411, 1761, 1806, 1863, 2278
Bbv12I GWGCWC 3 cut(s) 506, 764, 1109
BbvI GCAGC 9 cut(s) 598, 799, 1000, 1006, 1133, 1345, 1545, 1617, 1740
BccI CCATC 7 cut(s) 40, 265, 292, 1500, 1964, 2133, 2162
BceAI ACGGC 8 cut(s) 240, 691, 928, 1075, 1207, 1978, 2156, 2204
BcgI CGANNNNNNTGC 2 cut(s) 1771, 1805
BciT130I CCWGG 3 cut(s) 35, 1012, 1438
BcoDI GTCTC 5 cut(s) 642, 953, 1559, 1672, 2011
BfaI CTAG 5 cut(s) 561, 1218, 1254, 1880, 1970
BfmI CTRYAG 2 cut(s) 68, 986
BfoI RGCGCY 1 cut(s) 2270
BfuAI ACCTGC 1 cut(s) 1213
BglI GCCNNNNNGGC 1 cut(s) 1191
BglII AGATCT 4 cut(s) 72, 271, 620, 2227
BlnI CCTAGG 1 cut(s) 1217
Bme1390I CCNGG 3 cut(s) 35, 1012, 1438
Bme18I GGWCC 5 cut(s) 238, 375, 1313, 2037, 2113
BmeRI GACNNNNNGTC 2 cut(s) 943, 1510
BmeT110I CYCGRG 3 cut(s) 457, 872, 1931
BmgT120I GGNCC 6 cut(s) 238, 375, 1313, 1486, 2037, 2113
BmiI GGNNCC 7 cut(s) 228, 318, 597, 775, 795, 1247, 2268
BmrFI CCNGG 3 cut(s) 35, 1012, 1438
BmsI GCATC 1 cut(s) 915
BpiI GAAGAC 6 cut(s) 11, 1411, 1761, 1806, 1863, 2278
BplI GAGNNNNNCTC 2 cut(s) 1357, 1389
BpmI CTGGAG 3 cut(s) 56, 376, 994
Bpu14I TTCGAA 3 cut(s) 354, 978, 1791
Bsa29I ATCGAT 2 cut(s) 1322, 1429
BsaAI YACGTR 1 cut(s) 1819
BsaBI GATNNNNATC 1 cut(s) 283
BsaHI GRCGYC 3 cut(s) 438, 2169, 2267
BsaI GGTCTC 2 cut(s) 642, 2011
BsaJI CCNNGG 9 cut(s) 135, 404, 441, 458, 782, 1217, 1436, 1994, 2073
BsaWI WCCGGW 7 cut(s) 235, 313, 615, 663, 756, 1375, 1852
BsaXI ACNNNNNCTCC 6 cut(s) 286, 316, 746, 776, 1285, 1315
Bsc4I CCNNNNNNNGG 8 cut(s) 136, 180, 498, 783, 1157, 1494, 1495, 1498
Bse118I RCCGGY 4 cut(s) 494, 663, 1227, 2120
Bse1I ACTGG 5 cut(s) 13, 347, 420, 1066, 1715
Bse3DI GCAATG 2 cut(s) 745, 1779
Bse8I GATNNNNATC 1 cut(s) 283
BseAI TCCGGA 1 cut(s) 615
BseBI CCWGG 3 cut(s) 35, 1012, 1438
BseCI ATCGAT 2 cut(s) 1322, 1429
BseDI CCNNGG 9 cut(s) 135, 404, 441, 458, 782, 1217, 1436, 1994, 2073
BseGI GGATG 3 cut(s) 322, 2106, 2144
BseJI GATNNNNATC 1 cut(s) 283
BseLI CCNNNNNNNGG 8 cut(s) 136, 180, 498, 783, 1157, 1494, 1495, 1498
BseMI GCAATG 2 cut(s) 745, 1779
BseMII CTCAG 3 cut(s) 830, 1638, 1776
BseNI ACTGG 5 cut(s) 13, 347, 420, 1066, 1715
BseRI GAGGAG 7 cut(s) 294, 324, 1276, 1306, 1323, 1378, 1618
BseSI GKGCMC 1 cut(s) 1784
BseX3I CGGCCG 1 cut(s) 1229
BseXI GCAGC 9 cut(s) 598, 799, 1000, 1006, 1133, 1345, 1545, 1617, 1740
BsgI GTGCAG 1 cut(s) 737
Bsh1236I CGCG 6 cut(s) 181, 300, 1119, 1224, 1274, 1276
Bsh1285I CGRYCG 9 cut(s) 82, 245, 499, 757, 1057, 1077, 1228, 1232, 1326
BshFI GGCC 4 cut(s) 173, 999, 1231, 1487
BshNI GGYRCC 2 cut(s) 226, 2266
BshTI ACCGGT 1 cut(s) 663
BshVI ATCGAT 2 cut(s) 1322, 1429
BsiEI CGRYCG 9 cut(s) 82, 245, 499, 757, 1057, 1077, 1228, 1232, 1326
BsiHKAI GWGCWC 3 cut(s) 506, 764, 1109
BsiHKCI CYCGRG 3 cut(s) 457, 872, 1931
BslFI GGGAC 2 cut(s) 585, 2078
BslI CCNNNNNNNGG 8 cut(s) 136, 180, 498, 783, 1157, 1494, 1495, 1498
BsmAI GTCTC 5 cut(s) 642, 953, 1559, 1672, 2011
BsmBI CGTCTC 1 cut(s) 953
BsmFI GGGAC 2 cut(s) 585, 2078
BsmI GAATGC 3 cut(s) 1243, 1381, 2058
BsnI GGCC 4 cut(s) 173, 999, 1231, 1487
Bso31I GGTCTC 2 cut(s) 642, 2011
BsoBI CYCGRG 3 cut(s) 457, 872, 1931
Bsp119I TTCGAA 3 cut(s) 354, 978, 1791
Bsp1286I GDGCHC 5 cut(s) 506, 764, 776, 1109, 1784
Bsp13I TCCGGA 1 cut(s) 615
BspANI GGCC 4 cut(s) 173, 999, 1231, 1487
BspCNI CTCAG 3 cut(s) 829, 1639, 1777
BspDI ATCGAT 2 cut(s) 1322, 1429
BspEI TCCGGA 1 cut(s) 615
BspFNI CGCG 6 cut(s) 181, 300, 1119, 1224, 1274, 1276
BspHI TCATGA 1 cut(s) 1719
BspLI GGNNCC 7 cut(s) 228, 318, 597, 775, 795, 1247, 2268
BspMAI CTGCAG 1 cut(s) 990
BspMI ACCTGC 1 cut(s) 1213
BspPI GGATC 8 cut(s) 460, 590, 603, 788, 801, 1822, 1949, 2215
BspT104I TTCGAA 3 cut(s) 354, 978, 1791
BspT107I GGYRCC 2 cut(s) 226, 2266
BspTNI GGTCTC 2 cut(s) 642, 2011
BsrDI GCAATG 2 cut(s) 745, 1779
BsrFI RCCGGY 4 cut(s) 494, 663, 1227, 2120
BsrI ACTGG 5 cut(s) 13, 347, 420, 1066, 1715
BssAI RCCGGY 4 cut(s) 494, 663, 1227, 2120
BssECI CCNNGG 9 cut(s) 135, 404, 441, 458, 782, 1217, 1436, 1994, 2073
BssNI GRCGYC 3 cut(s) 438, 2169, 2267
BssSI CACGAG 3 cut(s) 370, 379, 2008
BssT1I CCWWGG 2 cut(s) 441, 1217
Bst2BI CACGAG 3 cut(s) 370, 379, 2008
Bst2UI CCWGG 3 cut(s) 35, 1012, 1438
Bst4CI ACNGT 7 cut(s) 145, 231, 433, 582, 660, 1317, 1459
Bst6I CTCTTC 2 cut(s) 342, 1581
BstACI GRCGYC 3 cut(s) 438, 2169, 2267
BstBAI YACGTR 1 cut(s) 1819
BstBI TTCGAA 3 cut(s) 354, 978, 1791
BstC8I GCNNGC 3 cut(s) 61, 1836, 2122
BstDEI CTNAG 3 cut(s) 816, 1647, 1785
BstDSI CCRYGG 1 cut(s) 1994
BstENI CCTNNNNNAGG 1 cut(s) 781
BstF5I GGATG 3 cut(s) 322, 2106, 2144
BstFNI CGCG 6 cut(s) 181, 300, 1119, 1224, 1274, 1276
BstH2I RGCGCY 1 cut(s) 2270
BstHHI GCGC 7 cut(s) 302, 835, 1008, 1121, 1224, 1276, 2269
BstMAI GTCTC 5 cut(s) 642, 953, 1559, 1672, 2011
BstMCI CGRYCG 9 cut(s) 82, 245, 499, 757, 1057, 1077, 1228, 1232, 1326
BstMWI GCNNNNNNNGC 8 cut(s) 60, 703, 744, 1005, 1191, 1228, 1539, 2126
BstNI CCWGG 3 cut(s) 35, 1012, 1438
BstSCI CCNGG 3 cut(s) 33, 1010, 1436
BstSFI CTRYAG 2 cut(s) 68, 986
BstSLI GKGCMC 1 cut(s) 1784
BstSNI TACGTA 1 cut(s) 1819
BstUI CGCG 6 cut(s) 181, 300, 1119, 1224, 1274, 1276
BstV1I GCAGC 9 cut(s) 598, 799, 1000, 1006, 1133, 1345, 1545, 1617, 1740
BstV2I GAAGAC 6 cut(s) 11, 1411, 1761, 1806, 1863, 2278
BstX2I RGATCY 8 cut(s) 72, 271, 465, 595, 620, 793, 1827, 2227
BstXI CCANNNNNNTGG 2 cut(s) 199, 829
BstYI RGATCY 8 cut(s) 72, 271, 465, 595, 620, 793, 1827, 2227
BstZI CGGCCG 1 cut(s) 1229
Bsu15I ATCGAT 2 cut(s) 1322, 1429
BsuRI GGCC 4 cut(s) 173, 999, 1231, 1487
BsuTUI ATCGAT 2 cut(s) 1322, 1429
BtgI CCRYGG 1 cut(s) 1994
BtgZI GCGATG 1 cut(s) 270
BtsCI GGATG 3 cut(s) 322, 2106, 2144
BtsIMutI CAGTG 2 cut(s) 413, 1782
BveI ACCTGC 1 cut(s) 1213
Cac8I GCNNGC 3 cut(s) 61, 1836, 2122
CaiI CAGNNNCTG 1 cut(s) 34
CciI TCATGA 1 cut(s) 1719
CfoI GCGC 7 cut(s) 302, 835, 1008, 1121, 1224, 1276, 2269
Cfr10I RCCGGY 4 cut(s) 494, 663, 1227, 2120
Cfr13I GGNCC 6 cut(s) 238, 375, 1313, 1486, 2037, 2113
ClaI ATCGAT 2 cut(s) 1322, 1429
CpoI CGGWCCG 1 cut(s) 238
CseI GACGC 3 cut(s) 446, 2135, 2177
Csp6I GTAC 3 cut(s) 661, 1441, 1591
CspAI ACCGGT 1 cut(s) 663
CspI CGGWCCG 1 cut(s) 238
CviAII CATG 4 cut(s) 55, 175, 1522, 1720
CviQI GTAC 3 cut(s) 661, 1441, 1591
DdeI CTNAG 3 cut(s) 816, 1647, 1785
DinI GGCGCC 1 cut(s) 2268
DrdI GACNNNNNNGTC 1 cut(s) 229
DriI GACNNNNNGTC 2 cut(s) 943, 1510
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 2 cut(s) 171, 1229
EagI CGGCCG 1 cut(s) 1229
Eam1104I CTCTTC 2 cut(s) 342, 1581
Eam1105I GACNNNNNGTC 2 cut(s) 943, 1510
EarI CTCTTC 2 cut(s) 342, 1581
EciI GGCGGA 4 cut(s) 496, 875, 1514, 1598
Ecl136II GAGCTC 3 cut(s) 504, 762, 1107
EclXI CGGCCG 1 cut(s) 1229
Eco105I TACGTA 1 cut(s) 1819
Eco130I CCWWGG 2 cut(s) 441, 1217
Eco147I AGGCCT 1 cut(s) 999
Eco24I GRGCYC 4 cut(s) 506, 764, 776, 1109
Eco31I GGTCTC 2 cut(s) 642, 2011
Eco32I GATATC 1 cut(s) 189
Eco47I GGWCC 5 cut(s) 238, 375, 1313, 2037, 2113
Eco52I CGGCCG 1 cut(s) 1229
Eco53kI GAGCTC 3 cut(s) 504, 762, 1107
Eco88I CYCGRG 3 cut(s) 457, 872, 1931
EcoICRI GAGCTC 3 cut(s) 504, 762, 1107
EcoNI CCTNNNNNAGG 1 cut(s) 781
EcoO109I RGGNCCY 3 cut(s) 375, 1486, 2113
EcoRI GAATTC 1 cut(s) 356
EcoRII CCWGG 3 cut(s) 33, 1010, 1436
EcoRV GATATC 1 cut(s) 189
EcoT14I CCWWGG 2 cut(s) 441, 1217
EcoT38I GRGCYC 4 cut(s) 506, 764, 776, 1109
EgeI GGCGCC 1 cut(s) 2268
EheI GGCGCC 1 cut(s) 2268
ErhI CCWWGG 2 cut(s) 441, 1217
Esp3I CGTCTC 1 cut(s) 953
FaeI CATG 4 cut(s) 58, 178, 1525, 1723
FalI AAGNNNNNCTT 5 cut(s) 833, 865, 959, 991, 2270
FaqI GGGAC 2 cut(s) 585, 2078
FatI CATG 4 cut(s) 54, 174, 1521, 1719
FauI CCCGC 4 cut(s) 174, 1827, 2049, 2090
FauNDI CATATG 1 cut(s) 2093
FblI GTMKAC 3 cut(s) 435, 672, 1815
FokI GGATG 3 cut(s) 309, 2113, 2151
FriOI GRGCYC 4 cut(s) 506, 764, 776, 1109
FspBI CTAG 5 cut(s) 561, 1218, 1254, 1880, 1970
GlaI GCGC 7 cut(s) 301, 834, 1007, 1120, 1223, 1275, 2268
GsuI CTGGAG 3 cut(s) 56, 376, 994
HaeII RGCGCY 1 cut(s) 2270
HaeIII GGCC 4 cut(s) 173, 999, 1231, 1487
HgaI GACGC 3 cut(s) 446, 2135, 2177
HhaI GCGC 7 cut(s) 302, 835, 1008, 1121, 1224, 1276, 2269
Hin1I GRCGYC 3 cut(s) 438, 2169, 2267
Hin1II CATG 4 cut(s) 58, 178, 1525, 1723
Hin6I GCGC 7 cut(s) 300, 833, 1006, 1119, 1222, 1274, 2267
HinP1I GCGC 7 cut(s) 300, 833, 1006, 1119, 1222, 1274, 2267
HincII GTYRAC 3 cut(s) 436, 673, 1926
HindII GTYRAC 3 cut(s) 436, 673, 1926
HphI GGTGA 7 cut(s) 225, 303, 537, 1106, 1194, 1222, 1951
Hpy166II GTNNAC 8 cut(s) 436, 643, 673, 898, 1114, 1515, 1816, 1926
Hpy8I GTNNAC 8 cut(s) 436, 643, 673, 898, 1114, 1515, 1816, 1926
HpyAV CCTTC 9 cut(s) 359, 805, 961, 1333, 1573, 1592, 1719, 1932, 1993
HpyCH4III ACNGT 7 cut(s) 145, 231, 433, 582, 660, 1317, 1459
HpyCH4IV ACGT 2 cut(s) 962, 1818
HpyCH4V TGCA 9 cut(s) 22, 54, 586, 718, 988, 1241, 1381, 1654, 2274
HpyF10VI GCNNNNNNNGC 8 cut(s) 60, 703, 744, 1005, 1191, 1228, 1539, 2126
HpyF3I CTNAG 3 cut(s) 816, 1647, 1785
HpySE526I ACGT 2 cut(s) 962, 1818
Hsp92I GRCGYC 3 cut(s) 438, 2169, 2267
Hsp92II CATG 4 cut(s) 58, 178, 1525, 1723
HspAI GCGC 7 cut(s) 300, 833, 1006, 1119, 1222, 1274, 2267
KasI GGCGCC 1 cut(s) 2266
Kpn2I TCCGGA 1 cut(s) 615
KroI GCCGGC 1 cut(s) 2120
KroNI GCCGGC 1 cut(s) 2122
Lsp1109I GCAGC 9 cut(s) 598, 799, 1000, 1006, 1133, 1345, 1545, 1617, 1740
LweI GCATC 1 cut(s) 915
MaeI CTAG 5 cut(s) 561, 1218, 1254, 1880, 1970
MaeII ACGT 2 cut(s) 962, 1818
MaeIII GTNAC 4 cut(s) 231, 309, 527, 1554
MfeI CAATTG 1 cut(s) 1985
MflI RGATCY 8 cut(s) 72, 271, 465, 595, 620, 793, 1827, 2227
MhlI GDGCHC 5 cut(s) 506, 764, 776, 1109, 1784
MluCI AATT 7 cut(s) 197, 356, 682, 727, 1033, 1965, 1985
Mly113I GGCGCC 1 cut(s) 2267
MmeI TCCRAC 8 cut(s) 144, 287, 479, 573, 1283, 1593, 1887, 2190
MroI TCCGGA 1 cut(s) 615
MroNI GCCGGC 1 cut(s) 2120
MseI TTAA 2 cut(s) 609, 1032
MslI CAYNNNNRTG 3 cut(s) 1520, 1899, 2159
MspA1I CMGCKG 1 cut(s) 1336
MspR9I CCNGG 3 cut(s) 35, 1012, 1438
MunI CAATTG 1 cut(s) 1985
Mva1269I GAATGC 3 cut(s) 1243, 1381, 2058
MvaI CCWGG 3 cut(s) 35, 1012, 1438
MvnI CGCG 6 cut(s) 181, 300, 1119, 1224, 1274, 1276
MwoI GCNNNNNNNGC 8 cut(s) 60, 703, 744, 1005, 1191, 1228, 1539, 2126
NaeI GCCGGC 1 cut(s) 2122
NarI GGCGCC 1 cut(s) 2267
NdeI CATATG 1 cut(s) 2093
NgoMIV GCCGGC 1 cut(s) 2120
NlaIII CATG 4 cut(s) 58, 178, 1525, 1723
NlaIV GGNNCC 7 cut(s) 228, 318, 597, 775, 795, 1247, 2268
NmeAIII GCCGAG 4 cut(s) 116, 836, 1128, 1588
NmuCI GTSAC 4 cut(s) 231, 309, 527, 1554
NspV TTCGAA 3 cut(s) 354, 978, 1791
PaeR7I CTCGAG 1 cut(s) 1931
PagI TCATGA 1 cut(s) 1719
PaqCI CACCTGC 1 cut(s) 1213
PceI AGGCCT 1 cut(s) 999
PcsI WCGNNNNNNNCGW 4 cut(s) 149, 377, 519, 2163
PctI GAATGC 3 cut(s) 1243, 1381, 2058
PdiI GCCGGC 1 cut(s) 2122
PfeI GAWTC 8 cut(s) 632, 933, 1128, 1349, 1426, 1508, 1700, 1793
PfoI TCCNGGA 1 cut(s) 1010
PinAI ACCGGT 1 cut(s) 663
Ple19I CGATCG 5 cut(s) 82, 245, 1057, 1077, 1326
PluTI GGCGCC 1 cut(s) 2270
Ppu21I YACGTR 1 cut(s) 1819
PpuMI RGGWCCY 2 cut(s) 375, 2113
PshBI ATTAAT 1 cut(s) 1032
Psp124BI GAGCTC 3 cut(s) 506, 764, 1109
Psp5II RGGWCCY 2 cut(s) 375, 2113
Psp6I CCWGG 3 cut(s) 33, 1010, 1436
PspGI CCWGG 3 cut(s) 33, 1010, 1436
PspN4I GGNNCC 7 cut(s) 228, 318, 597, 775, 795, 1247, 2268
PspPI GGNCC 6 cut(s) 238, 375, 1313, 1486, 2037, 2113
PspPPI RGGWCCY 2 cut(s) 375, 2113
PstI CTGCAG 1 cut(s) 990
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 8 cut(s) 72, 271, 465, 595, 620, 793, 1827, 2227
PvuI CGATCG 5 cut(s) 82, 245, 1057, 1077, 1326
RsaI GTAC 3 cut(s) 662, 1442, 1592
RsaNI GTAC 3 cut(s) 661, 1441, 1591
RseI CAYNNNNRTG 3 cut(s) 1520, 1899, 2159
Rsr2I CGGWCCG 1 cut(s) 238
RsrII CGGWCCG 1 cut(s) 238
SacI GAGCTC 3 cut(s) 506, 764, 1109
SalI GTCGAC 2 cut(s) 434, 671
SaqAI TTAA 2 cut(s) 609, 1032
Sau96I GGNCC 6 cut(s) 238, 375, 1313, 1486, 2037, 2113
ScrFI CCNGG 3 cut(s) 35, 1012, 1438
SduI GDGCHC 5 cut(s) 506, 764, 776, 1109, 1784
SfaNI GCATC 1 cut(s) 915
SfcI CTRYAG 2 cut(s) 68, 986
SfoI GGCGCC 1 cut(s) 2268
Sfr274I CTCGAG 1 cut(s) 1931
SfuI TTCGAA 3 cut(s) 354, 978, 1791
SgrDI CGTCGACG 2 cut(s) 434, 671
SinI GGWCC 5 cut(s) 238, 375, 1313, 2037, 2113
SlaI CTCGAG 1 cut(s) 1931
SmiMI CAYNNNNRTG 3 cut(s) 1520, 1899, 2159
SmlI CTYRAG 1 cut(s) 1931
SmoI CTYRAG 1 cut(s) 1931
SnaBI TACGTA 1 cut(s) 1819
Sse9I AATT 7 cut(s) 197, 356, 682, 727, 1033, 1965, 1985
SseBI AGGCCT 1 cut(s) 999
SspDI GGCGCC 1 cut(s) 2266
SspMI CTAG 5 cut(s) 561, 1218, 1254, 1880, 1970
SstI GAGCTC 3 cut(s) 506, 764, 1109
StuI AGGCCT 1 cut(s) 999
StyD4I CCNGG 3 cut(s) 33, 1010, 1436
StyI CCWWGG 2 cut(s) 441, 1217
TaaI ACNGT 7 cut(s) 145, 231, 433, 582, 660, 1317, 1459
TaiI ACGT 2 cut(s) 965, 1821
TaqII GACCGA 2 cut(s) 255, 1989
TasI AATT 7 cut(s) 197, 356, 682, 727, 1033, 1965, 1985
TatI WGTACW 1 cut(s) 1590
TauI GCSGC 8 cut(s) 482, 706, 1147, 1150, 1274, 1287, 1993, 2237
TfiI GAWTC 8 cut(s) 632, 933, 1128, 1349, 1426, 1508, 1700, 1793
Tru1I TTAA 2 cut(s) 609, 1032
Tru9I TTAA 2 cut(s) 609, 1032
TscAI CASTG 2 cut(s) 420, 1789
TseFI GTSAC 4 cut(s) 231, 309, 527, 1554
TseI GCWGC 9 cut(s) 586, 787, 988, 1019, 1121, 1333, 1533, 1630, 1728
Tsp45I GTSAC 4 cut(s) 231, 309, 527, 1554
TspDTI ATGAA 3 cut(s) 17, 1100, 1736
TspGWI ACGGA 6 cut(s) 114, 297, 528, 883, 1258, 2151
TspRI CASTG 2 cut(s) 420, 1789
VpaK11BI GGWCC 5 cut(s) 238, 375, 1313, 2037, 2113
VspI ATTAAT 1 cut(s) 1032
XagI CCTNNNNNAGG 1 cut(s) 781
XapI RAATTY 2 cut(s) 356, 1965
XbaI TCTAGA 1 cut(s) 1969
XhoI CTCGAG 1 cut(s) 1931
XmaJI CCTAGG 1 cut(s) 1217
XmiI GTMKAC 3 cut(s) 435, 672, 1815
XspI CTAG 5 cut(s) 561, 1218, 1254, 1880, 1970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.