Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0016210

Physical Location & Seq

Genomic Coordinates Reverse (-)

27510622 .. 27513570

Gene Structure 2949 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2949 bp

ATGCATTACACTCATTATGCGCTACAATATAGTCCCGCGCAGCGCTTGCGTTTGCCATGCACGTACTCGGCTAATGTAATGGCTACTTCAACCCGACACTACTGCTCCTCCACTTCCGCCGCCGTGCACCGCCATTCCATGTTGAAGAAAGCTCTGCCAAACCCGATTCTCCACTTCTCCAAACACCCTCAAAACCCCATTAAATTACCTTCCTTGAAGGAGGTTTGCGACGGAGGAAGCCTCAGGGAAGCTTTTCACTCACTCGGCAGTTTGCTCACGCATCCAAATTCCCTTCAGCTTTCTCTGGATGCGGCTTACTCGCCGGTCCTCGAGCTCTGCGCAAACGAGAAGGCTCTCTCAGAAGGGCAGCAAATCCACGCCCAGTTGATCAAATGTTGTGATGTGTCTGATTCTGCATTTCTGAGTACCAAGCTTGTTTTTATGTACGGGAAATGTGGCTCTCTTTTGGACGCGGAGAAGGTGTTTGATAAAATGTCCCACAGAACGATTTTCAGTTGGAACGCGATGATTGCTGCTTGTGCTTCAAACGGGAAGCCTTGGAAAGCTCTGGAGCTGTATAGGGACATGAGGGTTTTGGAGGTACCTCTCGATTCTTGCACTTTTCCTTGTATTCTTAAAGCGTGCGTTGCGGTTAACAATCTCCACTCTGGGGCAGAAATTCATGGTCTGGCTATCAAATACGGGTATAGTAATGTAACACTCGTTGTCAACTCACTAGCTTCGATGTATGCAAACTGCAAATGTGTTGACGGGGCGAGGAAGTTATTTGATGGTGTGAAGGAAAAAGAGGATATTGTGTCGTGGAATTCAATTATTTCAGCGTATTCCGCAAATGGGAAATCAGCTGAAGCATTGGGGCTGTTTCGAGAAATGCTAAGCTCGGGACTTGCCCTGAACTCATATACTCTTGTCGCTGCTCTTCAAGCCTGCGAGGATTCTTTTTGTGGTAAACTTGGTATGGAGATCCATGCTGCTGTGTTGAAATCAGGCCACTTCTTTGACATTTATGTGGCGAATTCTTTGCTTGCTATGTACGTAAGATGTGGTAACATGCATGAGGCTGCAATAATCTTCAGGGACTTGGATGCCAAGGATGTTGTTTCGTGGAATACCATGCTCTCTGGTTTTTCCCAGAATGGTCTATATGACGAAACACTGCTGTTGTTCCGAGATATGCAGAGTACTGATACAAAACCTGACCAGGTTTCACTATTAAATATCCTTGCAGCATCCGGTCGATTAGGAGATTTGTTGTCTGGGTTGGAAGCTCATGCTTATGCGATTAAAAAGGGATTCGACTCTGATTTGCAGCTCGGAAATACACTGATAGATATGTATGCTAGGTGTGGTTGTGTGAACTTCATGGGCCGTGCTTTTGACAAGATGCCTACTAAGGACTTAATTTCTTGGACAACTGTTATTGCTGGCTATGCTCAGAACAATTGTCACTTAAGGGCCTTAGAATTGTGCCGGAAGGTGCAGATGCTCCGATTGGATGCTGATGCAATGATGGTAGAAAGCATTTTAGCAGTTTGTGGGGCATTGAAATGTGTTTCTTTGGTAAAAGAAGCTCATGGTTACGCCGTGAGAAGAGGTTTATTCAATCTGGTGTTACAAAATGCAGTTGTCAACCTGTATGGAGAGTGTGTATATGTGAAATATGCAGAGCAGATGTTCGAATTGATTGACTCTAAGGATATTGTGTCTTGGACGAGCATGATCTCTTGTTATGTTCATAATGGGTTTGCAAATGAGGCCCTTGAACTATGCCACTTCATGAAAGAAACAAATGTTGAACCTGATGCTATAGCACTAGTTAGTATACTATCGGCTGTTGCGAGTTTATCTGCTCTGAAGAAAGGGAAAGAGATTCATGGTTTTCTTATCCGGAAGGGCTTCATCTTAGAGGGATCTCTTGGAAGCTCTCTAGTCGATATGTATGCCCGCTCTGGAACTTTGGAGAATGCATATGAGGTATATAACTGTATTAAAAATAGAAGTCTAATTTTGTGGACTACCATGATTAATGCGTATGGAATGCATGGCCAAGGCAAGGCAGCCATTGATTTATTCGAGCGGATGGAAGGTGAAAGAATTGTTCCTGATCATATTACGTTTTTGGCTCTTCTATATGCCTGCAGTCATTCGGGCTTGATTGATGAGGGTAAAAGAATATTTGAAATTATGAGAGATGATTATCATTTGGAACCATGGCCTGAACATTCTGCCTGTATGGTTGATCTTCTTGGTCGCGCCAATCGGTTGGAAGAGGCATACCATTTTGCGAATAGCATGCATAGTGAACCTACTGCTGAGGTATGGTGTGCACTCCTTGGGGCTTGCCGGGTTCATTCTAACAAAGAATTCGGAGAAATTGCAGCAAAGAAAATCTTAGAGTTGGACCCGGAGAATCCTGGAAATTATGTGCTTGTATCTAATGTGTTTGCTGCTAGTGGAAGATGGAAAGATGTAGATGAAGTGAGGTTGAGAATGAAGGGGAGTGGATTGAAGAAAAATCCGGGATGTAGTTGGATTGAGGTTGGAAACAAGGTTCATACATTCACAGCGAGGGATAAATCTCATCCGCAGTCCGATGATATTTACCTCAAGTTAGCTCAAATCACAGAGAAATTGGAGAGAGAAGTAGATTATGTGGCTCAAACCAAATTTGTTTTGCACAATGTAGAGGAAGAAGAGAGAGTTAAAATGCTTTATGGACATAGTGAAAGGCTGGCAATTGCATACGGTCTGCTTAAAACTCCCGAAGGAACCCCTATCCGAATCACCAAGAACCTTCGGGTTTGTGGCGATTGCCATCATTTCATTAAACTAGTTTCAAAAGTTTTTAGGCGGGTGCTTGTTGTGAGGGATGCAAATAGATTTCATCACTTTGAAGATGGTATCTGTTCTTGTGGCGATTTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

983

Amino Acids

109.34

Weight (kDa)

6.7

Isoelectric Point (pI)

37.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	169 - 216	2.5e-08	PPR repeat family
PPR	PF01535	171 - 198	6e-07	PPR repeat
PPR_2	PF13041	271 - 317	4.4e-10	PPR repeat family
PPR	PF01535	273 - 303	3.1e-08	PPR repeat
PPR_2	PF13041	371 - 410	6.6e-08	PPR repeat family
PPR	PF01535	374 - 401	3.9e-06	PPR repeat
PPR_2	PF13041	572 - 618	2.7e-07	PPR repeat family
PPR	PF01535	575 - 602	4.2e-06	PPR repeat
PPR_2	PF13041	676 - 721	8.4e-09	PPR repeat family
PPR	PF01535	677 - 705	2.8e-06	PPR repeat
E_motif	PF20431	791 - 853	1.6e-28	E motif
Eplus_motif	PF20430	858 - 885	5.8e-09	E+ motif
DYW_deaminase	PF14432	891 - 982	3.3e-44	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016210)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63370 AT3G63370
fragaria_vesca	FvH4_2g22910
malus_domestica	MD11G1278400.v1.1
prunus_persica	Prupe.8G234400_v2.0.a1
pyrus_communis	pycom11g24590
rosa_chinensis	RchiOBHm_Chr6g0289871
rosa_laevigata	RLG00000012263
rosa_multiflora	Rmu_ssc0000314.1_g000006
rosa_rugosa	Rorug06G0207400
rosa_samantha	Rh6AG319000 Rh6BG326000 Rh6CG331900 Rh6DG317400
rosa_wichuraiana	Rw6G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	340
Acc65I	GGTACC	1 cut(s)	601
AccB1I	GGYRCC	1 cut(s)	601
AccBSI	CCGCTC	2 cut(s)	1968, 2098
AccI	GTMKAC	1 cut(s)	1843
AccII	CGCG	4 cut(s)	38, 473, 524, 2274
AccIII	TCCGGA	1 cut(s)	1908
AclWI	GGATC	2 cut(s)	979, 1939
AcoI	YGGCCR	1 cut(s)	2065
AcsI	RAATTY	6 cut(s)	286, 678, 826, 1036, 2384, 2687
AcuI	CTGAAG	4 cut(s)	278, 888, 1078, 1895
AfaI	GTAC	6 cut(s)	65, 427, 446, 603, 1055, 1204
AfeI	AGCGCT	1 cut(s)	44
AfiI	CCNNNNNNNGG	3 cut(s)	670, 855, 2074
AflII	CTTAAG	1 cut(s)	1471
AhlI	ACTAGT	2 cut(s)	1834, 2851
AjnI	CCWGG	2 cut(s)	1221, 2434
AloI	GAACNNNNNNTCC	2 cut(s)	2556, 2588
Alw21I	GWGCWC	3 cut(s)	129, 336, 2350
Alw44I	GTGCAC	2 cut(s)	125, 2346
AlwI	GGATC	2 cut(s)	979, 1939
Ama87I	CYCGRG	2 cut(s)	329, 901
Aor13HI	TCCGGA	1 cut(s)	1908
Aor51HI	AGCGCT	1 cut(s)	44
AoxI	GGCC	6 cut(s)	1009, 1387, 1476, 1776, 2065, 2234
ApaLI	GTGCAC	2 cut(s)	125, 2346
ApoI	RAATTY	6 cut(s)	286, 678, 826, 1036, 2384, 2687
AseI	ATTAAT	1 cut(s)	2046
Asp700I	GAANNNNTTC	2 cut(s)	252, 1916
Asp718I	GGTACC	1 cut(s)	601
AspLEI	GCGC	5 cut(s)	22, 40, 45, 341, 2276
AspS9I	GGNCC	5 cut(s)	325, 1387, 1476, 1777, 2422
AsuC2I	CCSGG	3 cut(s)	2366, 2426, 2541
AsuHPI	GGTGA	2 cut(s)	2120, 2797
AsuII	TTCGAA	1 cut(s)	1698
AvaI	CYCGRG	2 cut(s)	329, 901
AvaII	GGWCC	2 cut(s)	325, 2422
AxyI	CCTNAGG	1 cut(s)	242
BaeGI	GKGCMC	2 cut(s)	129, 2350
BalI	TGGCCA	1 cut(s)	2067
BanI	GGYRCC	1 cut(s)	601
BanII	GRGCYC	1 cut(s)	336
Bbv12I	GWGCWC	3 cut(s)	129, 336, 2350
BbvCI	CCTCAGC	1 cut(s)	2334
BccI	CCATC	6 cut(s)	785, 1525, 2095, 2475, 2844, 2912
BceAI	ACGGC	3 cut(s)	107, 1374, 1589
BcgI	CGANNNNNNTGC	8 cut(s)	84, 118, 1024, 1058, 1943, 1977, 2296, 2330
BciT130I	CCWGG	2 cut(s)	1223, 2436
BclI	TGATCA	2 cut(s)	387, 2125
BcnI	CCSGG	3 cut(s)	2366, 2426, 2541
BcuI	ACTAGT	2 cut(s)	1834, 2851
BfaI	CTAG	6 cut(s)	737, 1362, 1835, 1949, 2472, 2852
BfmI	CTRYAG	2 cut(s)	1827, 2158
BfoI	RGCGCY	1 cut(s)	46
BfrI	CTTAAG	1 cut(s)	1471
BlpI	GCTNAGC	1 cut(s)	896
BmcAI	AGTACT	1 cut(s)	1204
Bme1390I	CCNGG	5 cut(s)	1223, 2366, 2426, 2436, 2541
Bme18I	GGWCC	2 cut(s)	325, 2422
BmeT110I	CYCGRG	2 cut(s)	329, 901
BmgT120I	GGNCC	5 cut(s)	325, 1387, 1476, 1777, 2422
BmiI	GGNNCC	4 cut(s)	603, 2229, 2424, 2791
BmrFI	CCNGG	5 cut(s)	1223, 2366, 2426, 2436, 2541
BmrI	ACTGGG	1 cut(s)	376
BmuI	ACTGGG	1 cut(s)	376
BplI	GAGNNNNNCTC	2 cut(s)	225, 257
BpmI	CTGGAG	1 cut(s)	590
Bpu10I	CCTNAGC	1 cut(s)	2334
Bpu1102I	GCTNAGC	1 cut(s)	896
Bpu14I	TTCGAA	1 cut(s)	1698
BpuEI	CTTGAG	1 cut(s)	2612
BpuMI	CCSGG	3 cut(s)	2366, 2426, 2541
BsaAI	YACGTR	2 cut(s)	63, 1057
BsaBI	GATNNNNATC	2 cut(s)	2217, 2835
BsaJI	CCNNGG	5 cut(s)	557, 1110, 2068, 2231, 2353
BsaWI	WCCGGW	2 cut(s)	1253, 1908
BsaXI	ACNNNNNCTCC	8 cut(s)	89, 92, 119, 122, 467, 497, 1653, 1683
Bsc4I	CCNNNNNNNGG	3 cut(s)	670, 855, 2074
Bse118I	RCCGGY	1 cut(s)	322
Bse1I	ACTGG	1 cut(s)	382
Bse21I	CCTNAGG	1 cut(s)	242
Bse3DI	GCAATG	1 cut(s)	1533
Bse8I	GATNNNNATC	2 cut(s)	2217, 2835
BseAI	TCCGGA	1 cut(s)	1908
BseBI	CCWGG	2 cut(s)	1223, 2436
BseDI	CCNNGG	5 cut(s)	557, 1110, 2068, 2231, 2353
BseJI	GATNNNNATC	2 cut(s)	2217, 2835
BseLI	CCNNNNNNNGG	3 cut(s)	670, 855, 2074
BseMI	GCAATG	1 cut(s)	1533
BseMII	CTCAG	5 cut(s)	256, 372, 413, 1469, 2325
BseNI	ACTGG	1 cut(s)	382
BseRI	GAGGAG	1 cut(s)	97
BseSI	GKGCMC	2 cut(s)	129, 2350
BsgI	GTGCAG	1 cut(s)	1520
Bsh1236I	CGCG	4 cut(s)	38, 473, 524, 2274
Bsh1285I	CGRYCG	1 cut(s)	1258
BshFI	GGCC	6 cut(s)	1011, 1389, 1478, 1778, 2067, 2236
BshNI	GGYRCC	1 cut(s)	601
BsiEI	CGRYCG	1 cut(s)	1258
BsiHKAI	GWGCWC	3 cut(s)	129, 336, 2350
BsiHKCI	CYCGRG	2 cut(s)	329, 901
BsiSI	CCGG	7 cut(s)	323, 1254, 1492, 1909, 2365, 2426, 2540
BslFI	GGGAC	5 cut(s)	18, 481, 596, 918, 1112
BslI	CCNNNNNNNGG	3 cut(s)	670, 855, 2074
BsmFI	GGGAC	5 cut(s)	18, 481, 596, 918, 1112
BsmI	GAATGC	2 cut(s)	1990, 2064
BsnI	GGCC	6 cut(s)	1011, 1389, 1478, 1778, 2067, 2236
BsoBI	CYCGRG	2 cut(s)	329, 901
Bsp119I	TTCGAA	1 cut(s)	1698
Bsp1286I	GDGCHC	3 cut(s)	129, 336, 2350
Bsp13I	TCCGGA	1 cut(s)	1908
Bsp143I	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
Bsp1720I	GCTNAGC	1 cut(s)	896
Bsp19I	CCATGG	1 cut(s)	2231
BspANI	GGCC	6 cut(s)	1011, 1389, 1478, 1778, 2067, 2236
BspCNI	CTCAG	5 cut(s)	255, 371, 414, 1468, 2326
BspEI	TCCGGA	1 cut(s)	1908
BspFNI	CGCG	4 cut(s)	38, 473, 524, 2274
BspHI	TCATGA	1 cut(s)	1797
BspLI	GGNNCC	4 cut(s)	603, 2229, 2424, 2791
BspMAI	CTGCAG	1 cut(s)	2162
BspPI	GGATC	2 cut(s)	979, 1939
BspQI	GCTCTTC	2 cut(s)	945, 2151
BspT104I	TTCGAA	1 cut(s)	1698
BspT107I	GGYRCC	1 cut(s)	601
BspTI	CTTAAG	1 cut(s)	1471
BsrBI	CCGCTC	2 cut(s)	1968, 2098
BsrDI	GCAATG	1 cut(s)	1533
BsrFI	RCCGGY	1 cut(s)	322
BsrI	ACTGG	1 cut(s)	382
BssAI	RCCGGY	1 cut(s)	322
BssECI	CCNNGG	5 cut(s)	557, 1110, 2068, 2231, 2353
BssMI	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
BssNAI	GTATAC	1 cut(s)	1844
BssT1I	CCWWGG	5 cut(s)	557, 1110, 2068, 2231, 2353
Bst1107I	GTATAC	1 cut(s)	1844
Bst2UI	CCWGG	2 cut(s)	1223, 2436
Bst4CI	ACNGT	3 cut(s)	1438, 2006, 2768
Bst6I	CTCTTC	5 cut(s)	945, 1606, 2151, 2283, 2709
BstAFI	CTTAAG	1 cut(s)	1471
BstAPI	GCANNNNNTGC	1 cut(s)	46
BstBAI	YACGTR	2 cut(s)	63, 1057
BstBI	TTCGAA	1 cut(s)	1698
BstDSI	CCRYGG	1 cut(s)	2231
BstFNI	CGCG	4 cut(s)	38, 473, 524, 2274
BstH2I	RGCGCY	1 cut(s)	46
BstHHI	GCGC	5 cut(s)	22, 40, 45, 341, 2276
BstKTI	GATC	6 cut(s)	390, 987, 1743, 1934, 2128, 2263
BstMBI	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
BstMCI	CGRYCG	1 cut(s)	1258
BstMWI	GCNNNNNNNGC	8 cut(s)	46, 530, 539, 647, 848, 944, 1451, 1775
BstNI	CCWGG	2 cut(s)	1223, 2436
BstNSI	RCATGY	2 cut(s)	1075, 2317
BstSCI	CCNGG	5 cut(s)	1221, 2364, 2424, 2434, 2539
BstSFI	CTRYAG	2 cut(s)	1827, 2158
BstSLI	GKGCMC	2 cut(s)	129, 2350
BstSNI	TACGTA	1 cut(s)	1057
BstUI	CGCG	4 cut(s)	38, 473, 524, 2274
BstX2I	RGATCY	2 cut(s)	984, 1931
BstXI	CCANNNNNNTGG	1 cut(s)	2284
BstYI	RGATCY	2 cut(s)	984, 1931
BstZ17I	GTATAC	1 cut(s)	1844
Bsu36I	CCTNAGG	1 cut(s)	242
BsuRI	GGCC	6 cut(s)	1011, 1389, 1478, 1778, 2067, 2236
BtgI	CCRYGG	1 cut(s)	2231
BtgZI	GCGATG	1 cut(s)	539
BtsI	GCAGTG	1 cut(s)	1175
BtsIMutI	CAGTG	2 cut(s)	1175, 1343
CciI	TCATGA	1 cut(s)	1797
CfoI	GCGC	5 cut(s)	22, 40, 45, 341, 2276
Cfr10I	RCCGGY	1 cut(s)	322
Cfr13I	GGNCC	5 cut(s)	325, 1387, 1476, 1777, 2422
CseI	GACGC	1 cut(s)	479
CsiI	ACCWGGT	1 cut(s)	1221
Csp6I	GTAC	6 cut(s)	64, 426, 445, 602, 1054, 1203
CviQI	GTAC	6 cut(s)	64, 426, 445, 602, 1054, 1203
DpnI	GATC	6 cut(s)	389, 986, 1742, 1933, 2127, 2262
DpnII	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
EaeI	YGGCCR	1 cut(s)	2065
Eam1104I	CTCTTC	5 cut(s)	945, 1606, 2151, 2283, 2709
EarI	CTCTTC	5 cut(s)	945, 1606, 2151, 2283, 2709
EciI	GGCGGA	1 cut(s)	106
Ecl136II	GAGCTC	1 cut(s)	334
Eco105I	TACGTA	1 cut(s)	1057
Eco130I	CCWWGG	5 cut(s)	557, 1110, 2068, 2231, 2353
Eco24I	GRGCYC	1 cut(s)	336
Eco47I	GGWCC	2 cut(s)	325, 2422
Eco47III	AGCGCT	1 cut(s)	44
Eco53kI	GAGCTC	1 cut(s)	334
Eco57I	CTGAAG	4 cut(s)	278, 888, 1078, 1895
Eco81I	CCTNAGG	1 cut(s)	242
Eco88I	CYCGRG	2 cut(s)	329, 901
EcoICRI	GAGCTC	1 cut(s)	334
EcoO109I	RGGNCCY	2 cut(s)	1476, 1777
EcoRI	GAATTC	3 cut(s)	826, 1036, 2384
EcoRII	CCWGG	2 cut(s)	1221, 2434
EcoT14I	CCWWGG	5 cut(s)	557, 1110, 2068, 2231, 2353
EcoT22I	ATGCAT	5 cut(s)	6, 1077, 1990, 2064, 2319
EcoT38I	GRGCYC	1 cut(s)	336
ErhI	CCWWGG	5 cut(s)	557, 1110, 2068, 2231, 2353
FalI	AAGNNNNNCTT	2 cut(s)	2396, 2428
FaqI	GGGAC	5 cut(s)	18, 481, 596, 918, 1112
FauI	CCCGC	3 cut(s)	43, 1973, 2865
FauNDI	CATATG	1 cut(s)	1990
FbaI	TGATCA	2 cut(s)	387, 2125
FblI	GTMKAC	1 cut(s)	1843
FriOI	GRGCYC	1 cut(s)	336
FspBI	CTAG	6 cut(s)	737, 1362, 1835, 1949, 2472, 2852
FspI	TGCGCA	1 cut(s)	340
GlaI	GCGC	5 cut(s)	21, 39, 44, 340, 2275
GsuI	CTGGAG	1 cut(s)	590
HaeII	RGCGCY	1 cut(s)	46
HaeIII	GGCC	6 cut(s)	1011, 1389, 1478, 1778, 2067, 2236
HapII	CCGG	7 cut(s)	323, 1254, 1492, 1909, 2365, 2426, 2540
HgaI	GACGC	1 cut(s)	479
HhaI	GCGC	5 cut(s)	22, 40, 45, 341, 2276
Hin6I	GCGC	5 cut(s)	20, 38, 43, 339, 2274
HinP1I	GCGC	5 cut(s)	20, 38, 43, 339, 2274
HincII	GTYRAC	4 cut(s)	655, 730, 769, 1651
HindII	GTYRAC	4 cut(s)	655, 730, 769, 1651
HindIII	AAGCTT	2 cut(s)	249, 431
HpaI	GTTAAC	1 cut(s)	655
HpaII	CCGG	7 cut(s)	323, 1254, 1492, 1909, 2365, 2426, 2540
HphI	GGTGA	2 cut(s)	2120, 2797
Hpy99I	CGWCG	1 cut(s)	233
HpyCH4III	ACNGT	3 cut(s)	1438, 2006, 2768
HpyCH4IV	ACGT	3 cut(s)	62, 1056, 2135
HpyF10VI	GCNNNNNNNGC	8 cut(s)	46, 530, 539, 647, 848, 944, 1451, 1775
HpySE526I	ACGT	3 cut(s)	62, 1056, 2135
HspAI	GCGC	5 cut(s)	20, 38, 43, 339, 2274
Kpn2I	TCCGGA	1 cut(s)	1908
KpnI	GGTACC	1 cut(s)	605
Ksp22I	TGATCA	2 cut(s)	387, 2125
KspAI	GTTAAC	1 cut(s)	655
Kzo9I	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
LguI	GCTCTTC	2 cut(s)	945, 2151
LmnI	GCTCC	3 cut(s)	110, 571, 1512
MabI	ACCWGGT	1 cut(s)	1221
MaeI	CTAG	6 cut(s)	737, 1362, 1835, 1949, 2472, 2852
MaeII	ACGT	3 cut(s)	62, 1056, 2135
MaeIII	GTNAC	5 cut(s)	715, 1067, 1466, 1598, 1632
MalI	GATC	6 cut(s)	389, 986, 1742, 1933, 2127, 2262
MbiI	CCGCTC	2 cut(s)	1968, 2098
MboI	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
MfeI	CAATTG	2 cut(s)	1462, 2757
MflI	RGATCY	2 cut(s)	984, 1931
MhlI	GDGCHC	3 cut(s)	129, 336, 2350
MlsI	TGGCCA	1 cut(s)	2067
MluNI	TGGCCA	1 cut(s)	2067
MlyI	GAGTC	2 cut(s)	1313, 1703
MmeI	TCCRAC	6 cut(s)	497, 1263, 2265, 2400, 2531, 2542
Mox20I	TGGCCA	1 cut(s)	2067
Mph1103I	ATGCAT	5 cut(s)	6, 1077, 1990, 2064, 2319
MroI	TCCGGA	1 cut(s)	1908
MroXI	GAANNNNTTC	2 cut(s)	252, 1916
MscI	TGGCCA	1 cut(s)	2067
MslI	CAYNNNNRTG	3 cut(s)	1028, 1296, 1567
Msp20I	TGGCCA	1 cut(s)	2067
MspA1I	CMGCKG	1 cut(s)	866
MspCI	CTTAAG	1 cut(s)	1471
MspI	CCGG	7 cut(s)	323, 1254, 1492, 1909, 2365, 2426, 2540
MspR9I	CCNGG	5 cut(s)	1223, 2366, 2426, 2436, 2541
MteI	GCGCNGCGC	1 cut(s)	41
MunI	CAATTG	2 cut(s)	1462, 2757
Mva1269I	GAATGC	2 cut(s)	1990, 2064
MvaI	CCWGG	2 cut(s)	1223, 2436
MvnI	CGCG	4 cut(s)	38, 473, 524, 2274
MwoI	GCNNNNNNNGC	8 cut(s)	46, 530, 539, 647, 848, 944, 1451, 1775
NciI	CCSGG	3 cut(s)	2366, 2426, 2541
NcoI	CCATGG	1 cut(s)	2231
NdeI	CATATG	1 cut(s)	1990
NdeII	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
NlaIV	GGNNCC	4 cut(s)	603, 2229, 2424, 2791
NmeAIII	GCCGAG	2 cut(s)	47, 243
NmuCI	GTSAC	1 cut(s)	1466
NsbI	TGCGCA	1 cut(s)	340
NsiI	ATGCAT	5 cut(s)	6, 1077, 1990, 2064, 2319
NspI	RCATGY	2 cut(s)	1075, 2317
NspV	TTCGAA	1 cut(s)	1698
PaeI	GCATGC	1 cut(s)	2317
PaeR7I	CTCGAG	1 cut(s)	329
PagI	TCATGA	1 cut(s)	1797
PciSI	GCTCTTC	2 cut(s)	945, 2151
PcsI	WCGNNNNNNNCGW	1 cut(s)	1856
PctI	GAATGC	2 cut(s)	1990, 2064
PdmI	GAANNNNTTC	2 cut(s)	252, 1916
PfeI	GAWTC	8 cut(s)	166, 410, 611, 956, 1314, 1891, 2431, 2802
PfoI	TCCNGGA	2 cut(s)	2434, 2539
PleI	GAGTC	2 cut(s)	1313, 1703
PpsI	GAGTC	2 cut(s)	1313, 1703
Ppu21I	YACGTR	2 cut(s)	63, 1057
PshBI	ATTAAT	1 cut(s)	2046
Psp124BI	GAGCTC	1 cut(s)	336
Psp6I	CCWGG	2 cut(s)	1221, 2434
PspGI	CCWGG	2 cut(s)	1221, 2434
PspN4I	GGNNCC	4 cut(s)	603, 2229, 2424, 2791
PspPI	GGNCC	5 cut(s)	325, 1387, 1476, 1777, 2422
PspXI	VCTCGAGB	1 cut(s)	329
PstI	CTGCAG	1 cut(s)	2162
PsuI	RGATCY	2 cut(s)	984, 1931
PvuII	CAGCTG	1 cut(s)	866
RsaI	GTAC	6 cut(s)	65, 427, 446, 603, 1055, 1204
RsaNI	GTAC	6 cut(s)	64, 426, 445, 602, 1054, 1203
RseI	CAYNNNNRTG	3 cut(s)	1028, 1296, 1567
SacI	GAGCTC	1 cut(s)	336
SapI	GCTCTTC	2 cut(s)	945, 2151
Sau3AI	GATC	6 cut(s)	387, 984, 1740, 1931, 2125, 2260
Sau96I	GGNCC	5 cut(s)	325, 1387, 1476, 1777, 2422
ScaI	AGTACT	1 cut(s)	1204
SchI	GAGTC	2 cut(s)	1313, 1703
ScrFI	CCNGG	5 cut(s)	1223, 2366, 2426, 2436, 2541
SduI	GDGCHC	3 cut(s)	129, 336, 2350
SexAI	ACCWGGT	1 cut(s)	1221
SfcI	CTRYAG	2 cut(s)	1827, 2158
Sfr274I	CTCGAG	1 cut(s)	329
SfuI	TTCGAA	1 cut(s)	1698
SinI	GGWCC	2 cut(s)	325, 2422
SlaI	CTCGAG	1 cut(s)	329
SmiMI	CAYNNNNRTG	3 cut(s)	1028, 1296, 1567
SmlI	CTYRAG	3 cut(s)	329, 1471, 2627
SmoI	CTYRAG	3 cut(s)	329, 1471, 2627
SnaBI	TACGTA	1 cut(s)	1057
SpeI	ACTAGT	2 cut(s)	1834, 2851
SphI	GCATGC	1 cut(s)	2317
SspI	AATATT	1 cut(s)	2196
SspMI	CTAG	6 cut(s)	737, 1362, 1835, 1949, 2472, 2852
SstI	GAGCTC	1 cut(s)	336
StyD4I	CCNGG	5 cut(s)	1221, 2364, 2424, 2434, 2539
StyI	CCWWGG	5 cut(s)	557, 1110, 2068, 2231, 2353
TaaI	ACNGT	3 cut(s)	1438, 2006, 2768
TaiI	ACGT	3 cut(s)	65, 1059, 2138
TaqI	TCGA	9 cut(s)	330, 609, 743, 886, 1258, 1317, 1698, 1953, 2094
TatI	WGTACW	1 cut(s)	1202
TauI	GCSGC	2 cut(s)	122, 314
TfiI	GAWTC	8 cut(s)	166, 410, 611, 956, 1314, 1891, 2431, 2802
TscAI	CASTG	2 cut(s)	1182, 1350
TseFI	GTSAC	1 cut(s)	1466
Tsp45I	GTSAC	1 cut(s)	1466
TspGWI	ACGGA	1 cut(s)	246
TspRI	CASTG	2 cut(s)	1182, 1350
Vha464I	CTTAAG	1 cut(s)	1471
VneI	GTGCAC	2 cut(s)	125, 2346
VpaK11BI	GGWCC	2 cut(s)	325, 2422
VspI	ATTAAT	1 cut(s)	2046
XapI	RAATTY	6 cut(s)	286, 678, 826, 1036, 2384, 2687
XceI	RCATGY	2 cut(s)	1075, 2317
XhoI	CTCGAG	1 cut(s)	329
XmiI	GTMKAC	1 cut(s)	1843
XmnI	GAANNNNTTC	2 cut(s)	252, 1916
XspI	CTAG	6 cut(s)	737, 1362, 1835, 1949, 2472, 2852
ZrmI	AGTACT	1 cut(s)	1204
Zsp2I	ATGCAT	5 cut(s)	6, 1077, 1990, 2064, 2319

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.