pycom11g25890

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013011
Physical Location & Seq
Reverse (-)
28580723 .. 28582910
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGAGAAAGCGGATTAAGCCTCGAACCGAATTGAGCACATATGGTGTCATCCTCCTCCTCCGGCTGTTCGGCAAGATTCGGAAAAGGGGAGAATTTTTCAGCGGCCGGCGAAGCACGAAATGGACGGCCGAGAAACGACTAAAACGTCGTCGTCCAAGGCTATTTGCGACAAGTTCGATTGATGTATATGCTGCACAATGTGCAGAATGCAAGAAATGGAGAGTTATTCATTCAGAAGAAGAGTTTGAGGAAATCAGGAGCAAAATCAGTACGCAACCCTTTTATTGCAACAAGAAATCTGGTGTGACTTGTGATGATGTTGCCGATATTGAGTACAACGCTACTAGGACCTGGGTCATTGATAAGCCTGACCTTCCGAAGACCCCAGAAGGTTTCAAGAGACACTTGGTGGTTAGAAAAGATTATTCCAAACTGGATGCGTATTATATTACACCCACGGGGAAAAGATTGAGATCACAAAATCAGGTGGCAGCGTTTTTAAAAGAAAACCCCATGTTTGAAGGCATATCCGCTTCAGATTTTGACTTTGCAGCCCCAAAGGTAATGGAAGACACCGTTCCTGAGATTTTTGGTAAAAAGGGTTCTGATAGCAGCCGCAAAAAAATTAAAACGAAAAAGAATGAGGATCAAAGTGCTGATATCGCTGGTGAAGAAGATGTTGAGTAG

Protein Analysis

229

Amino Acids

26.39

Weight (kDa)

9.6

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CW PF07496 63 - 107 8.9e-08 CW-type Zinc Finger
MBD PF01429 120 - 190 6.6e-17 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63030
fragaria_vesca FvH4_2g24220
malus_domestica MD03G1271900.v1.1 MD11G1292500.v1.1
prunus_persica Prupe.8G248100_v2.0.a1
pyrus_communis pycom03g21650 pycom11g25890
rosa_chinensis RchiOBHm_Chr6g0292001
rosa_laevigata RLG00000012071
rosa_multiflora Rmu_sc0008666.1_g000005
rosa_roxburghii Rroxscaffold_7G00175500 Rroxscaffold_7G00178020
rosa_rugosa Rorug06G0222400
rosa_samantha Rh6AG336200 Rh6BG343600 Rh6CG349800 Rh6DG336600
rosa_wichuraiana Rw6G029270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 144
AciI CCGC 4 cut(s) 10, 102, 531, 616
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 2 cut(s) 103, 126
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 519
AdeI CACNNNGTG 2 cut(s) 200, 409
AfaI GTAC 2 cut(s) 271, 335
AgsI TTSAA 2 cut(s) 397, 521
AjnI CCWGG 1 cut(s) 350
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 394
AlwI GGATC 1 cut(s) 654
AoxI GGCC 2 cut(s) 103, 126
ApeKI GCWGC 4 cut(s) 191, 491, 551, 612
ApoI RAATTY 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 1 cut(s) 680
AvaII GGWCC 1 cut(s) 348
BbsI GAAGAC 2 cut(s) 386, 576
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 4 cut(s) 178, 503, 563, 624
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 394
BfaI CTAG 1 cut(s) 345
BisI GCNGC 6 cut(s) 103, 192, 492, 552, 613, 616
BlsI GCNGC 6 cut(s) 104, 193, 493, 553, 614, 617
Bme1390I CCNGG 1 cut(s) 352
Bme18I GGWCC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 348
BmrFI CCNGG 1 cut(s) 352
BmsI GCATC 1 cut(s) 427
BoxI GACNNNNGTC 1 cut(s) 353
BpiI GAAGAC 2 cut(s) 386, 576
BsaBI GATNNNNATC 1 cut(s) 472
BsaJI CCNNGG 3 cut(s) 155, 351, 456
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 472
BseBI CCWGG 1 cut(s) 352
BseDI CCNNGG 3 cut(s) 155, 351, 456
BseGI GGATG 2 cut(s) 48, 442
BseJI GATNNNNATC 1 cut(s) 472
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 438
BseRI GAGGAG 2 cut(s) 44, 47
BseX3I CGGCCG 2 cut(s) 103, 126
BseXI GCAGC 4 cut(s) 178, 503, 563, 624
BsgI GTGCAG 2 cut(s) 177, 222
Bsh1285I CGRYCG 2 cut(s) 106, 129
BshFI GGCC 2 cut(s) 105, 128
BsiEI CGRYCG 2 cut(s) 106, 129
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 2 cut(s) 61, 106
BsmAI GTCTC 1 cut(s) 394
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 2 cut(s) 105, 128
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 2 cut(s) 473, 646
BspACI CCGC 4 cut(s) 10, 102, 531, 616
BspANI GGCC 2 cut(s) 105, 128
BspCNI CTCAG 1 cut(s) 574
BspPI GGATC 1 cut(s) 654
BsrFI RCCGGY 1 cut(s) 105
BsrI ACTGG 1 cut(s) 438
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 3 cut(s) 155, 351, 456
BssMI GATC 2 cut(s) 473, 646
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 582
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 2 cut(s) 48, 442
BstKTI GATC 2 cut(s) 476, 649
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 2 cut(s) 473, 646
BstMCI CGRYCG 2 cut(s) 106, 129
BstMWI GCNNNNNNNGC 4 cut(s) 16, 111, 200, 662
BstNI CCWGG 1 cut(s) 352
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 350
BstV1I GCAGC 4 cut(s) 178, 503, 563, 624
BstV2I GAAGAC 2 cut(s) 386, 576
BstZI CGGCCG 2 cut(s) 103, 126
BsuRI GGCC 2 cut(s) 105, 128
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 2 cut(s) 48, 442
Cac8I GCNNGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 2 cut(s) 270, 334
CviAII CATG 1 cut(s) 514
CviJI RGCY 8 cut(s) 19, 64, 105, 128, 160, 367, 554, 615
CviKI_1 RGCY 8 cut(s) 19, 64, 105, 128, 160, 367, 554, 615
CviQI GTAC 2 cut(s) 270, 334
DdeI CTNAG 1 cut(s) 582
DpnI GATC 2 cut(s) 475, 648
DpnII GATC 2 cut(s) 473, 646
DraI TTTAAA 1 cut(s) 501
DraIII CACNNNGTG 2 cut(s) 200, 409
DrdI GACNNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 144
EaeI YGGCCR 2 cut(s) 103, 126
EagI CGGCCG 2 cut(s) 103, 126
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EclXI CGGCCG 2 cut(s) 103, 126
Eco130I CCWWGG 1 cut(s) 155
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 348
Eco52I CGGCCG 2 cut(s) 103, 126
Eco57I CTGAAG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 350
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 1 cut(s) 517
FaiI YATR 7 cut(s) 40, 42, 187, 189, 447, 515, 527
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FatI CATG 1 cut(s) 513
FauNDI CATATG 1 cut(s) 40
Fnu4HI GCNGC 6 cut(s) 103, 192, 492, 552, 613, 616
FokI GGATG 2 cut(s) 35, 449
Fsp4HI GCNGC 6 cut(s) 103, 192, 492, 552, 613, 616
FspBI CTAG 1 cut(s) 345
GluI GCNGC 6 cut(s) 103, 192, 492, 552, 613, 616
HaeIII GGCC 2 cut(s) 105, 128
HapII CCGG 2 cut(s) 61, 106
Hin1II CATG 1 cut(s) 517
HinfI GANTC 1 cut(s) 76
HpaII CCGG 2 cut(s) 61, 106
HphI GGTGA 1 cut(s) 680
Hpy188I TCNGA 5 cut(s) 81, 235, 378, 538, 607
Hpy188III TCNNGA 3 cut(s) 256, 397, 581
Hpy99I CGWCG 2 cut(s) 150, 153
HpyAV CCTTC 3 cut(s) 383, 383, 515
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 5 cut(s) 194, 203, 210, 288, 551
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 111, 200, 662
HpyF3I CTNAG 1 cut(s) 582
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 1 cut(s) 517
KroI GCCGGC 1 cut(s) 105
KroNI GCCGGC 1 cut(s) 107
Kzo9I GATC 2 cut(s) 473, 646
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 4 cut(s) 178, 503, 563, 624
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 345
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 475, 648
MboI GATC 2 cut(s) 473, 646
MboII GAAGA 6 cut(s) 248, 251, 391, 581, 683, 686
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 3 cut(s) 29, 92, 624
MnlI CCTC 6 cut(s) 30, 62, 65, 68, 241, 637
MroNI GCCGGC 1 cut(s) 105
MseI TTAA 3 cut(s) 15, 500, 627
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 2 cut(s) 61, 106
MspR9I CCNGG 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 212
MvaI CCWGG 1 cut(s) 352
MwoI GCNNNNNNNGC 4 cut(s) 16, 111, 200, 662
NaeI GCCGGC 1 cut(s) 107
NdeI CATATG 1 cut(s) 40
NdeII GATC 2 cut(s) 473, 646
NgoMIV GCCGGC 1 cut(s) 105
NlaIII CATG 1 cut(s) 517
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PctI GAATGC 1 cut(s) 212
PdiI GCCGGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 6 cut(s) 104, 193, 493, 553, 614, 617
PpuMI RGGWCCY 1 cut(s) 348
PshAI GACNNNNGTC 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspPI GGNCC 1 cut(s) 348
PspPPI RGGWCCY 1 cut(s) 348
PsrI GAACNNNNNNTAC 2 cut(s) 586, 618
RsaI GTAC 2 cut(s) 271, 335
RsaNI GTAC 2 cut(s) 270, 334
SaqAI TTAA 3 cut(s) 15, 500, 627
SatI GCNGC 6 cut(s) 103, 192, 492, 552, 613, 616
Sau3AI GATC 2 cut(s) 473, 646
Sau96I GGNCC 1 cut(s) 348
ScrFI CCNGG 1 cut(s) 352
SduI GDGCHC 1 cut(s) 38
SetI ASST 6 cut(s) 148, 353, 375, 394, 489, 564
SfaNI GCATC 1 cut(s) 427
SinI GGWCC 1 cut(s) 348
Sse9I AATT 3 cut(s) 29, 92, 624
SsiI CCGC 4 cut(s) 10, 102, 531, 616
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 350
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 577
TaiI ACGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 22, 176
TasI AATT 3 cut(s) 29, 92, 624
TatI WGTACW 1 cut(s) 333
TauI GCSGC 2 cut(s) 105, 618
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 3 cut(s) 15, 500, 627
Tru9I TTAA 3 cut(s) 15, 500, 627
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 191, 491, 551, 612
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 348
XapI RAATTY 1 cut(s) 92
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.