pycom12419g00180

Protein BIG GRAIN 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012419
OG0013871
Physical Location & Seq
Reverse (-)
341631 .. 342989
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12419g00180.1

Sequence Viewer

Length: 1359 bp
ATGGAGAGGTGGGATATCAAATCACTCCCGAAAAACAGAGAAGGCCGCTCCAACCGACACAGCAGAGACAACCCATCTTTCTCTTCCACCCTCCTCGACTCCATTTACCGTTCCATCGACGAACCCATTGTCGGCGGCGATGAAGTGGTTTCGATGAGAGAAGAACAGCGCCACCTCATTTTCTACAAAGAAACCATGAAGAAAAAACAGAGCACCGCCGCCGCCACCACTACCAGCGGCCGACTAGGCCATGGACACTACAAAGAAGACCAGGAGCGTGAATTAGCGAACCTCCGACGAGCCTGCTTGGTCGAGAAATGGATGGAGAAGAAGGCAGCTGAGAAGTCCGTACCTGTGCGACGAAATTCCATGGCGGATTTCCCGACGACAAAGTCTAGATACAGTCACCAGAACGAGTTCTTGACGAATTCTAGTTCCAGTTCTTCAGATTCCAGTGGTGGGTTTTCTTCGTCGGAGTCGGACTCCATGTACGGATCAAAATCGAGGTCCTCATCGTCATGCTACAGCATGCACAGGCCGAAGCCAATTCGAACCAGCCTTTCGTCAGAAAAGGTTCCATTTGACGATCACCAGAGAAATTACCATCACAATTCACAGAAGAAGCACGAGAATGGGTTCGTGAAGACGAAATCAAAAGCGCTGAAGATTTACAGCGATTTGAAGAAGGTGAAGCAGCCGATTTCGCCAGGTGGTCGGCTAGCCAGTTTTCTCAATTCTCTGTTCAATGCAGGGCACGTGAAGAAGTCCAAAATTGACGATTTGGGTGCGGAGAGAAAATCGTCACCAAATTCGGGACAAATGGGGTACTCAAACCCGAGTAGTAGTTGTTCGTCAGCCTCTTCGTTTTCAAGGTCTTGCTTGCGAAAAGCGCCTCTTTCGAGAAGCGAGTTGAGTGGCATCAGCAGCAATGGTGATGCCGCAAAGAGGTCGGTGAGGTTTTGTCCTGTAAGTGTGATTGTGGATGAGGATTGCCGCCCGTGCGGACAAAAGACTCTACTTGAAGCACAGTCGGGTTTAATGGCCGCAGCTAAAGCTGCAGCGGCCATTAAACCGCCAACAAATGAAGATGTTGGACTTGAGTGTTGTGTAATGGATGTTGATGAAGACGATGACCATGGCCGAAGAATCGAGGAAGTTGCGAGAGATTACTTGATGAAGAATTATCAGAAGAAGCATGATGAGGATGATGAGGATGATGATGCAGAGAGCTATGCAAGTTCTGATCTCTTTGAGTTGGATACTGCTGGGGTTGAAGAAAGGTACCGTGAAGAATTGCCTGTGTATGAGACTACCTTTTTCGACAGAAATCGAGCCATTGCTAATGCTTTGATCTTGTAA

Protein Analysis

453

Amino Acids

50.56

Weight (kDa)

7.97

Isoelectric Point (pI)

62.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54200 AT3G13980
fragaria_vesca FvH4_6g34500
malus_domestica MD09G1185600.v1.1 MD17G1170300.v1.1
prunus_persica Prupe.3G043400_v2.0.a1
pyrus_communis pycom09g10290 pycom12419g00180
rosa_chinensis RchiOBHm_Chr2g0144811
rosa_laevigata RLG00000020115
rosa_multiflora Rmu_sc0002354.1_g000024
rosa_roxburghii Rroxscaffold_2G00100950
rosa_rugosa Rorug02G0390000
rosa_samantha Rh2AG441700 Rh2BG452300 Rh2DG462100
rosa_wichuraiana Rw2G036120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
Acc65I GGTACC 1 cut(s) 1281
AccB1I GGYRCC 1 cut(s) 1281
AccBSI CCGCTC 1 cut(s) 48
AclWI GGATC 1 cut(s) 502
AcoI YGGCCR 4 cut(s) 238, 1041, 1062, 1138
AcsI RAATTY 3 cut(s) 364, 427, 808
AcuI CTGAAG 2 cut(s) 429, 683
AcvI CACGTG 1 cut(s) 757
AfaI GTAC 4 cut(s) 351, 491, 827, 1283
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 4 cut(s) 131, 459, 812, 945
AgsI TTSAA 5 cut(s) 682, 745, 870, 1022, 1274
AjnI CCWGG 2 cut(s) 270, 706
AluBI AGCT 4 cut(s) 338, 1049, 1055, 1230
AluI AGCT 4 cut(s) 338, 1049, 1055, 1230
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 2 cut(s) 60, 1301
AlwI GGATC 1 cut(s) 502
Ama87I CYCGRG 1 cut(s) 835
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 7 cut(s) 43, 238, 247, 536, 1041, 1062, 1138
ApeKI GCWGC 6 cut(s) 335, 694, 924, 1046, 1055, 1058
ApoI RAATTY 3 cut(s) 364, 427, 808
ArsI GACNNNNNNTTYG 4 cut(s) 114, 146, 491, 523
Asp700I GAANNNNTTC 3 cut(s) 416, 573, 635
Asp718I GGTACC 1 cut(s) 1281
AspLEI GCGC 3 cut(s) 171, 661, 892
AspS9I GGNCC 1 cut(s) 507
AsuHPI GGTGA 6 cut(s) 398, 581, 700, 795, 944, 964
AsuII TTCGAA 1 cut(s) 550
AsuNHI GCTAGC 1 cut(s) 718
AvaI CYCGRG 1 cut(s) 835
AvaII GGWCC 1 cut(s) 507
BaeGI GKGCMC 1 cut(s) 756
BanI GGYRCC 1 cut(s) 1281
BauI CACGAG 1 cut(s) 626
BbrPI CACGTG 1 cut(s) 757
BbsI GAAGAC 3 cut(s) 273, 650, 1131
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 6 cut(s) 347, 706, 936, 1042, 1058, 1070
BccI CCATC 4 cut(s) 82, 122, 316, 612
BcgI CGANNNNNNTGC 8 cut(s) 285, 319, 767, 801, 930, 964, 1139, 1173
BciT130I CCWGG 2 cut(s) 272, 708
BciVI GTATCC 1 cut(s) 1252
BcoDI GTCTC 2 cut(s) 60, 1301
BfaI CTAG 4 cut(s) 245, 396, 432, 719
BfmI CTRYAG 2 cut(s) 523, 1056
BfoI RGCGCY 3 cut(s) 172, 662, 893
BfuI GTATCC 1 cut(s) 1252
BglI GCCNNNNNGGC 1 cut(s) 246
Bme1390I CCNGG 2 cut(s) 272, 708
Bme18I GGWCC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 835
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 2 cut(s) 576, 1283
BmrFI CCNGG 2 cut(s) 272, 708
BmsI GCATC 3 cut(s) 925, 927, 1210
BmtI GCTAGC 1 cut(s) 722
BpiI GAAGAC 3 cut(s) 273, 650, 1131
BplI GAGNNNNNCTC 2 cut(s) 467, 499
Bpu14I TTCGAA 1 cut(s) 550
BpuEI CTTGAG 1 cut(s) 1118
BsaAI YACGTR 1 cut(s) 757
BsaBI GATNNNNATC 1 cut(s) 499
BsaJI CCNNGG 3 cut(s) 250, 369, 1135
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 459, 812, 945
Bse1I ACTGG 3 cut(s) 438, 453, 723
Bse3DI GCAATG 2 cut(s) 934, 1335
Bse8I GATNNNNATC 1 cut(s) 499
BseBI CCWGG 2 cut(s) 272, 708
BseDI CCNNGG 3 cut(s) 250, 369, 1135
BseGI GGATG 5 cut(s) 327, 988, 1120, 1210, 1219
BseJI GATNNNNATC 1 cut(s) 499
BseLI CCNNNNNNNGG 4 cut(s) 131, 459, 812, 945
BseMI GCAATG 2 cut(s) 934, 1335
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 3 cut(s) 438, 453, 723
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 756
BseX3I CGGCCG 1 cut(s) 238
BseXI GCAGC 6 cut(s) 347, 706, 936, 1042, 1058, 1070
BseYI CCCAGC 1 cut(s) 1265
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 7 cut(s) 45, 240, 249, 538, 1043, 1064, 1140
BshNI GGYRCC 1 cut(s) 1281
BsiEI CGRYCG 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 835
BslFI GGGAC 1 cut(s) 828
BslI CCNNNNNNNGG 4 cut(s) 131, 459, 812, 945
BsmAI GTCTC 2 cut(s) 60, 1301
BsmFI GGGAC 1 cut(s) 828
BsnI GGCC 7 cut(s) 45, 240, 249, 538, 1043, 1064, 1140
BsoBI CYCGRG 1 cut(s) 835
Bsp119I TTCGAA 1 cut(s) 550
Bsp1286I GDGCHC 2 cut(s) 215, 756
Bsp143I GATC 4 cut(s) 494, 586, 1243, 1350
Bsp19I CCATGG 3 cut(s) 250, 369, 1135
BspANI GGCC 7 cut(s) 45, 240, 249, 538, 1043, 1064, 1140
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 576, 1283
BspMAI CTGCAG 1 cut(s) 1060
BspOI GCTAGC 1 cut(s) 722
BspPI GGATC 1 cut(s) 502
BspT104I TTCGAA 1 cut(s) 550
BspT107I GGYRCC 1 cut(s) 1281
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 934, 1335
BsrI ACTGG 3 cut(s) 438, 453, 723
BssECI CCNNGG 3 cut(s) 250, 369, 1135
BssMI GATC 4 cut(s) 494, 586, 1243, 1350
BssSI CACGAG 1 cut(s) 626
BssT1I CCWWGG 3 cut(s) 250, 369, 1135
Bst2BI CACGAG 1 cut(s) 626
Bst2UI CCWGG 2 cut(s) 272, 708
Bst4CI ACNGT 4 cut(s) 110, 404, 1029, 1286
Bst6I CTCTTC 2 cut(s) 88, 865
BstBAI YACGTR 1 cut(s) 757
BstBI TTCGAA 1 cut(s) 550
BstC8I GCNNGC 4 cut(s) 304, 530, 720, 881
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 3 cut(s) 250, 369, 1135
BstF5I GGATG 5 cut(s) 327, 988, 1120, 1210, 1219
BstH2I RGCGCY 3 cut(s) 172, 662, 893
BstHHI GCGC 3 cut(s) 171, 661, 892
BstKTI GATC 4 cut(s) 497, 589, 1246, 1353
BstMAI GTCTC 2 cut(s) 60, 1301
BstMBI GATC 4 cut(s) 494, 586, 1243, 1350
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 8 cut(s) 246, 703, 889, 924, 999, 1052, 1055, 1061
BstNI CCWGG 2 cut(s) 272, 708
BstNSI RCATGY 1 cut(s) 532
BstSCI CCNGG 2 cut(s) 270, 706
BstSFI CTRYAG 2 cut(s) 523, 1056
BstSLI GKGCMC 1 cut(s) 756
BstV1I GCAGC 6 cut(s) 347, 706, 936, 1042, 1058, 1070
BstV2I GAAGAC 3 cut(s) 273, 650, 1131
BstZI CGGCCG 1 cut(s) 238
BsuI GTATCC 1 cut(s) 1252
BsuRI GGCC 7 cut(s) 45, 240, 249, 538, 1043, 1064, 1140
BtgI CCRYGG 3 cut(s) 250, 369, 1135
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 5 cut(s) 327, 988, 1120, 1210, 1219
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 4 cut(s) 304, 530, 720, 881
CfoI GCGC 3 cut(s) 171, 661, 892
Cfr13I GGNCC 1 cut(s) 507
Csp6I GTAC 4 cut(s) 350, 490, 826, 1282
CviAII CATG 8 cut(s) 196, 251, 370, 487, 519, 529, 1136, 1196
CviQI GTAC 4 cut(s) 350, 490, 826, 1282
DdeI CTNAG 1 cut(s) 339
DpnI GATC 4 cut(s) 496, 588, 1245, 1352
DpnII GATC 4 cut(s) 494, 586, 1243, 1350
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 4 cut(s) 238, 1041, 1062, 1138
EagI CGGCCG 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 88, 865
EarI CTCTTC 2 cut(s) 88, 865
EciI GGCGGA 1 cut(s) 389
EclXI CGGCCG 1 cut(s) 238
Eco130I CCWWGG 3 cut(s) 250, 369, 1135
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 507
Eco47III AGCGCT 1 cut(s) 660
Eco52I CGGCCG 1 cut(s) 238
Eco57I CTGAAG 2 cut(s) 429, 683
Eco72I CACGTG 1 cut(s) 757
Eco88I CYCGRG 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 507
EcoRI GAATTC 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 270, 706
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 3 cut(s) 250, 369, 1135
ErhI CCWWGG 3 cut(s) 250, 369, 1135
FaeI CATG 8 cut(s) 199, 254, 373, 490, 522, 532, 1139, 1199
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 1 cut(s) 828
FatI CATG 8 cut(s) 195, 250, 369, 486, 518, 528, 1135, 1195
FokI GGATG 5 cut(s) 334, 995, 1127, 1217, 1226
FspBI CTAG 4 cut(s) 245, 396, 432, 719
GlaI GCGC 3 cut(s) 170, 660, 891
GsaI CCCAGC 1 cut(s) 1269
HaeII RGCGCY 3 cut(s) 172, 662, 893
HaeIII GGCC 7 cut(s) 45, 240, 249, 538, 1043, 1064, 1140
HhaI GCGC 3 cut(s) 171, 661, 892
Hin1II CATG 8 cut(s) 199, 254, 373, 490, 522, 532, 1139, 1199
Hin6I GCGC 3 cut(s) 169, 659, 890
HinP1I GCGC 3 cut(s) 169, 659, 890
HinfI GANTC 6 cut(s) 98, 449, 476, 482, 1012, 1146
HphI GGTGA 6 cut(s) 398, 581, 700, 795, 944, 964
Hpy188I TCNGA 7 cut(s) 296, 448, 475, 481, 568, 1188, 1243
Hpy188III TCNNGA 8 cut(s) 28, 313, 382, 396, 421, 640, 813, 900
Hpy99I CGWCG 5 cut(s) 122, 300, 363, 388, 475
HpyAV CCTTC 3 cut(s) 35, 325, 679
HpyCH4III ACNGT 4 cut(s) 110, 404, 1029, 1286
HpyCH4IV ACGT 1 cut(s) 756
HpyCH4V TGCA 5 cut(s) 532, 749, 1058, 1223, 1235
HpyF10VI GCNNNNNNNGC 8 cut(s) 246, 703, 889, 924, 999, 1052, 1055, 1061
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 1 cut(s) 756
Hsp92II CATG 8 cut(s) 199, 254, 373, 490, 522, 532, 1139, 1199
HspAI GCGC 3 cut(s) 169, 659, 890
KpnI GGTACC 1 cut(s) 1285
Kzo9I GATC 4 cut(s) 494, 586, 1243, 1350
LmnI GCTCC 2 cut(s) 53, 274
Lsp1109I GCAGC 6 cut(s) 347, 706, 936, 1042, 1058, 1070
LweI GCATC 3 cut(s) 925, 927, 1210
MaeI CTAG 4 cut(s) 245, 396, 432, 719
MaeII ACGT 1 cut(s) 756
MaeIII GTNAC 2 cut(s) 404, 801
MalI GATC 4 cut(s) 496, 588, 1245, 1352
MbiI CCGCTC 1 cut(s) 48
MboI GATC 4 cut(s) 494, 586, 1243, 1350
MhlI GDGCHC 2 cut(s) 215, 756
MlyI GAGTC 4 cut(s) 92, 476, 485, 1006
MmeI TCCRAC 6 cut(s) 75, 319, 453, 459, 1072, 1236
MroXI GAANNNNTTC 3 cut(s) 416, 573, 635
MseI TTAA 2 cut(s) 1037, 1068
MslI CAYNNNNRTG 2 cut(s) 517, 630
MspA1I CMGCKG 3 cut(s) 237, 338, 1061
MspR9I CCNGG 2 cut(s) 272, 708
MvaI CCWGG 2 cut(s) 272, 708
MwoI GCNNNNNNNGC 8 cut(s) 246, 703, 889, 924, 999, 1052, 1055, 1061
NcoI CCATGG 3 cut(s) 250, 369, 1135
NdeII GATC 4 cut(s) 494, 586, 1243, 1350
NheI GCTAGC 1 cut(s) 718
NlaIII CATG 8 cut(s) 199, 254, 373, 490, 522, 532, 1139, 1199
NlaIV GGNNCC 2 cut(s) 576, 1283
NmuCI GTSAC 2 cut(s) 404, 801
NspI RCATGY 1 cut(s) 532
NspV TTCGAA 1 cut(s) 550
PaeI GCATGC 1 cut(s) 532
PdmI GAANNNNTTC 3 cut(s) 416, 573, 635
PfeI GAWTC 2 cut(s) 449, 1146
PflFI GACNNNGTC 1 cut(s) 391
PleI GAGTC 4 cut(s) 92, 476, 484, 1006
PmaCI CACGTG 1 cut(s) 757
PmlI CACGTG 1 cut(s) 757
PpsI GAGTC 4 cut(s) 92, 476, 484, 1006
Ppu21I YACGTR 1 cut(s) 757
PpuMI RGGWCCY 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 507
Psp6I CCWGG 2 cut(s) 270, 706
PspCI CACGTG 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 1265
PspGI CCWGG 2 cut(s) 270, 706
PspN4I GGNNCC 2 cut(s) 576, 1283
PspPI GGNCC 1 cut(s) 507
PspPPI RGGWCCY 1 cut(s) 507
PsrI GAACNNNNNNTAC 2 cut(s) 832, 864
PstI CTGCAG 1 cut(s) 1060
PsyI GACNNNGTC 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 4 cut(s) 351, 491, 827, 1283
RsaNI GTAC 4 cut(s) 350, 490, 826, 1282
RseI CAYNNNNRTG 2 cut(s) 517, 630
SaqAI TTAA 2 cut(s) 1037, 1068
Sau3AI GATC 4 cut(s) 494, 586, 1243, 1350
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 4 cut(s) 92, 476, 485, 1006
ScrFI CCNGG 2 cut(s) 272, 708
SduI GDGCHC 2 cut(s) 215, 756
SfaNI GCATC 3 cut(s) 925, 927, 1210
SfcI CTRYAG 2 cut(s) 523, 1056
SfiI GGCCNNNNNGGCC 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 550
SinI GGWCC 1 cut(s) 507
SmiMI CAYNNNNRTG 2 cut(s) 517, 630
SmlI CTYRAG 1 cut(s) 1097
SmoI CTYRAG 1 cut(s) 1097
SphI GCATGC 1 cut(s) 532
SspMI CTAG 4 cut(s) 245, 396, 432, 719
StyD4I CCNGG 2 cut(s) 270, 706
StyI CCWWGG 3 cut(s) 250, 369, 1135
TaaI ACNGT 4 cut(s) 110, 404, 1029, 1286
TaiI ACGT 1 cut(s) 759
TauI GCSGC 9 cut(s) 48, 138, 221, 224, 240, 941, 996, 1046, 1064
TfiI GAWTC 2 cut(s) 449, 1146
Tru1I TTAA 2 cut(s) 1037, 1068
Tru9I TTAA 2 cut(s) 1037, 1068
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 2 cut(s) 404, 801
TseI GCWGC 6 cut(s) 335, 694, 924, 1046, 1055, 1058
Tsp45I GTSAC 2 cut(s) 404, 801
TspDTI ATGAA 5 cut(s) 156, 212, 1098, 1137, 1190
TspGWI ACGGA 2 cut(s) 337, 507
TspRI CASTG 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 507
XapI RAATTY 3 cut(s) 364, 427, 808
XbaI TCTAGA 1 cut(s) 395
XceI RCATGY 1 cut(s) 532
XmnI GAANNNNTTC 3 cut(s) 416, 573, 635
XspI CTAG 4 cut(s) 245, 396, 432, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.